Aburo Seun - Academia.edu (original) (raw)

Papers by Aburo Seun

BMC complementary medicine and therapies, Jun 19, 2023

Background Reports have implicated diabetes mellitus (DM) and Alzheimer's disease (AD) as some of... more Background Reports have implicated diabetes mellitus (DM) and Alzheimer's disease (AD) as some of the global persistent health challenges with no lasting solutions, despite of significant inputs of modern-day pharmaceutical firms. This study therefore, aimed to appraise the in vitro antioxidant potential, enzymes inhibitory activities, and as well carry out in silico study on bioactive compounds from polyphenolic-rich extract of Hibiscus cannabinus seed (PEHc). Methods In vitro antioxidant assays were performed on PEHc using standard methods while the identification of phytoconstituents was carried out with high performance liquid chromatography (HPLC). For the in silico molecular docking using Schrodinger's Grid-based ligand docking with energetics software, seven target proteins were retrieved from the database (https:// www. rcsb. org/). Results HPLC technique identified twelve chemical compounds in PEHc, while antioxidant quantification revealed higher total phenolic contents (243.5 ± 0.71 mg GAE/g) than total flavonoid contents (54.06 ± 0.09 mg QE/g) with a significant (p < 0.05) inhibition of ABTS (IC 50 = 218.30 ± 0.87 µg/ml) and 1, 1-diphenyl-2-picrylhydrazyl free radicals (IC 50 = 227.79 ± 0.74 µg/ml). In a similar manner, the extract demonstrated a significant (p < 0.05) inhibitory activity against α-amylase (IC 50 = 256.88 ± 6.15 µg/ml) and α-glucosidase (IC 50 = 183.19 ± 0.23 µg/ml) as well as acetylcholinesterase (IC 50 = 262.95 ± 1.47 µg/ml) and butyrylcholinesterase (IC 50 = 189.97 ± 0.82 µg/ml), respectively. Furthermore, In silico study showed that hibiscetin (a lead) revealed a very strong binding affinity energies for DPP-4, (PDB ID: 1RWQ) and α-amylase (PDB ID: 1SMD), gamma-tocopherol

IntechOpen eBooks, Jun 28, 2023

Respiratory failure, characterized as the unsuccessful maintenance of adequate gas exchange, is a... more Respiratory failure, characterized as the unsuccessful maintenance of adequate gas exchange, is associated with abnormalities of arterial blood gas tensions. The coronavirus disease-2019 (COVID-19) is majorly a respiratory disease capable of causing infection caused by the newly discovered coronavirus (SARS-CoV-2) with a consequential effect on respiratory failure. Simply put, respiratory failure is the major clinical demonstration of COVID-19 and the frontline cause of the associated mortality. Respiratory failure instigated by COVID-19 has some clinical features in affected patients. Disorders of the respiratory neuromuscular, airway, pulmonary vesicles, and lung parenchyma all manifest in COVID-19. These features are heterogeneous and categorized into progressive respiratory distress and unique "silent hypoxemia" as two phenotypes. Knowing the exact phenotype in patients with COVID-19 has been of important clinical significance in seeking the right treatment strategies for treating respiratory failure. The chapter will, therefore, provide more insights into the pathophysiology, clinical attributes, pathogenesis, and treatment approach of respiratory failure in COVID-19 conditions, as well as evaluate any similarities and differences that may exist.

Journal of molecular pathophysiology, 2017

Background/Aim: The effects of several household materials containing toxic chemicals have been a... more Background/Aim: The effects of several household materials containing toxic chemicals have been a focus point in recent studies concerning male infertility. In this study, we evaluated the testiculotoxic effect of isopropyl alcohol-based air freshener (IBA) on the testis of adult Wistar rats. Materials and Methods: About 20 adult Wistar rats were divided at random, to four groups, five in each group. Group A rats were exposed to natural air for 4 weeks. The study groups (B, C, and D) were exposed to different graded doses of IBA for 4 weeks. Group B rats were exposed to 2.5 ml, Group C to 5 ml, and Group D to 10 ml of IBA for 6 h/day via whole body inhalation. Results: The results of the study showed a statistically significant (P < 0.05) decrease in testis weight, testis relative body weight and testis volume in the IBA exposed groups as compared to the control group. Reduction in sperm concentration, sperm motility, normal sperm morphology and significant (P < 0.05) increase in abnormal sperm morphology was observed in the study groups when compared to that of the control group. Furthermore, in comparison to the control group, degeneration of testicular architecture was observed in the study groups. Conclusion: Exposure of IBA to adult Wistar rats causes testicular histology damage and deranged sperm parameters.

Journal of Food Biochemistry, Jun 5, 2023

Rising concerns with the use of synthetic antidiabetic drugs have promoted a shift towards the us... more Rising concerns with the use of synthetic antidiabetic drugs have promoted a shift towards the use of natural products. Tis study therefore investigated the antidiabetic activity of peptide hydrolysate of Telfairia occidentalis (T. occidentalis) seed protein (PHTOSP) in streptozotocin-induced diabetic rats. Tirty-six (36) experimental animals were randomly distributed into six groups (A-F) of six rats each (n = 6). Group A served as normal control while groups B, C, D, E, and F were treated with streptozotocin (STZ, 50 mg/kg body weight, b.w, i.p) dissolved in cold citrate bufer (0.1 M, pH 4.5) to induce type 2 diabetes. Groups C, D, and E were administered 50, 100, and 150 mg/kg b.w PHTOSP, respectively, while groups B and F were diabetic untreated and 5 mg/kg b.w glibenclamide-treated controls, respectively, in the experiment that lasted for 21 days. Subsequently, the analysis of biochemical parameters demonstrated a signifcant (p < 0.05) increase in serum insulin, hepatic glycogen, hexokinase, and glucose-6-phosphate dehydrogenase activities, accompanied by a signifcant (p < 0.05) reduction in fasting serum glucose, glucose-6-phosphatase, and fructose-1,6-bisphosphatase, along with an enhancement in relative body weight. Similarly, PHTOSP demonstrated a signifcant (p < 0.05) improvement on high-density (HDL) and low-density (LDL) lipoproteins, triglyceride (TG), total cholesterol (TC), and atherogenic index (AI) signifcantly (p < 0.05). In addition, histoarchitectural analysis revealed a reversal of congestion and proliferation of infammatory cells in the pancreatic tissue following treatment with PHTOSP. Terefore, PHTOSP might possess potential antidiabetic properties such that it improves glycolytic pathway and promotes cell survival that are helpful in the management of diabetes mellitus (DM).

Journal of bionanoscience, Feb 1, 2018

BMC Complementary and Alternative Medicine, 2019

Background: Ocimum gratissimum L. is a medicinal plant widely grown in tropical and subtropical r... more Background: Ocimum gratissimum L. is a medicinal plant widely grown in tropical and subtropical regions with the leaf decoction usually taken in folk medicine to enhance erectile performance in men although the probable mechanism of actions remains undetermined. This study examined the inhibitory potentials of Ocimum gratissimum leaves on some key enzymes associated with erectile dysfunction in penile and testicular tissues of the rat. Methods: Inhibitory effect of aqueous extract (1:10 w/v) of O. gratissimum leaves on the activities of phosphodiesterase-5 (PDE-5), arginase, angiotensin I-converting enzyme (ACE), and acetylcholinesterase (AChE) in penile and testicular tissues were assessed. Also, the extract was investigated for ferric reducing antioxidant property(FRAP) and 1,1-diphenyl-2-picrylhydrazil (DPPH) radical scavenging abilities. Results: The extract showed higher PDE-5 (IC 50 = 43.19 μg/mL), ACE (IC 50 = 44.23 μg/mL), AChE (IC 50 = 55.51 μg/mL) and arginase (IC 50 = 46.12 μg/mL) inhibitory activity in the penile tissue than PDE-5 (IC 50 = 44.67 μg/mL), ACE (IC 50 = 53.99 μg/ mL), AChE (IC 50 = 60.03 μg/mL) and arginase (IC 50 = 49.12 μg/mL) inhibitory activity in the testicular tissue homogenate. Furthermore, the extract scavenged free radicals and in a dose-dependent manner. Conclusion: The enzyme activities displayed might be associated with the bioactive compounds present in the extract which could possibly explain its use in the management of erectile dysfunction (ED).

$Research paper thumbnail of Inhibitory effect of Bryophyllum pinnatum (Lam.) Oken leaf extract and their fractions on α-amylase, α-glucosidase and cholinesterase enzyme$

Pharmacognosy Journal, 2018

Bryophyllum pinnatum (Lam.) Oken leaves are employed as food and as traditional medicines. This s... more Bryophyllum pinnatum (Lam.) Oken leaves are employed as food and as traditional medicines. This study investigates the antioxidant activity (reducing power, DPPH, ABTS, FRAP, H 2 O 2 scavenging ability and metal ion chelating), carbohydrate digesting enzymes activity and inhibitory activity of cholinergic enzyme of aqueous extract and fractions (n-hexane, ethyl acetate, n-butanol, residual aqueous fraction) of B. pinnatum leaves were investigated. Results showed that aqueous extract of B. pinnatum exhibited DPPH radical scavenging abilities, iron chelation, hydrogen peroxide scavenging abilities and reducing power (Fe 3+-Fe 2+). B. pinnatum aqueous extract also had considerably α-amylase and α-glucosidase inhibitory activities with IC 50 values 149.20 ± 14.44 µg/mL and 126.15 ± 9.76 µg/mL respectively. Our findings indicated that ethyl acetate fraction contained a considerably higher (p < 0.05) amount of total phenolic, flavonoids, total antioxidant, FRAP, metal ion, ABTS and DPPH radical scavenging activity than other solvent fractions. Furthermore, the ethyl acetate fraction elicited a significantly higher (p < 0.05) inhibitory effects on α-glucosidase (IC 50 = 70.90 ± 1.23 μg/ml), α-amylase (IC 50 = 62.45 ± 1.22 μg/ml), acetylcholinesterase (AChE) (IC 50 = 66.75 µg/mL) and butyrylcholinesterase (BChE) (IC 50 = 62.97 µg/mL) activities than other fractions. Hence, B. pinnatum leaves were rich in biologically active components; thus, could be employed to formulate new plant-based pharmaceutical and nutraceutical drugs to improve human health.

$Research paper thumbnail of Identification of bioactive constituents of chloroform fraction from <i>Annona muricata</i> leaf, its antioxidant activity and inhibitory potential against carbohydrate-hydrolyzing α-amylase and α-glucosidase activities linked to type II diabetes mellitus: <i>In vitro</i> study$

Journal of HerbMed Pharmacology, Dec 31, 2022

Chloroform fraction from Annona muricata leaf revealed some important phytochemical constituents ... more Chloroform fraction from Annona muricata leaf revealed some important phytochemical constituents and demonstrated significant antidiabetic potentials that might be useful as an alternative therapy in the management of diabetes mellitus. Please cite this paper as: Olasehinde OR, Afolabi OB. Identification of bioactive constituents of chloroform fraction from Annona muricata leaf, its antioxidant activity and inhibitory potential against carbohydrate-hydrolyzing α-amylase and α-glucosidase activities linked to type II diabetes mellitus: In vitro study.

Toxicology Reports, 2021

Over time, diabetes patients usually need combination therapy involving two or more agents, inclu... more Over time, diabetes patients usually need combination therapy involving two or more agents, including phytonutrients to attain therapeutic targets. The purpose of this research is to elucidate the combined effect of metformin and gallic acid (GA) on glucose metabolism, inflammation as well as oxidative and endoplasmic reticulum (ER) stresses in fructose-fed diabetic rats. Thirty-five rats of Wistar strain were arbitrarily distributed into five groups, each containing seven animals as follows: normal control, diabetic control, groups administered 100 mg/kg bw metformin only, 50 mg/kg bw gallic acid only and a combination of both. Experimental animals were made diabetic by single injection of 40 mg/kg streptozotocin (intraperitoneally) subsequent to 14 days administration of 10 % fructose prior. Treatment of rats continued for 21 days following diabetes confirmation. Glucose and insulin levels as well as lipid profile were evaluated in the serum, while activities of catalase and superoxide dismutase were estimated in both liver and pancreas. In addition, levels of malondialdehyde, interleukin-6 and tumor necrosis factor-alpha, as well as expression of activating transcription factor-4 were evaluated in liver and pancreas of diabetic rats. Activities of glucose-6-phosphatase and glucokinase were also determined in liver of diabetic animals. Metformin only, GA only and combination of metformin and GA significantly improved antioxidant status and glucose homeostasis while inflammation and endoplasmic reticulum stress were significantly ameliorated in diabetic rats. Metformin/GA combination appeared to improve glucose metabolism by increasing insulin level and ameliorating the dysregulated activities of glucose metabolizing enzymes and ER stress better than either metformin only or GA only. It could be concluded that coadministration of metformin/GA produced a combined effect in ameliorating diabetes in Wistar rats and could be considered in treatment of diabetes.

Journal of Diabetes & Metabolic Disorders, 2020

FOREWORD 'Sorry, no Free Reading' 1 'Reading is important because if you read you understand and ... more FOREWORD 'Sorry, no Free Reading' 1 'Reading is important because if you read you understand and voant to read again.' (Mabel Mbavu, aged 13, Glen Norah High School.

Journal of Herbmed Pharmacology, 2021

Introduction: Diabetes mellitus (DM) has been recognized as the seventh leading cause of global m... more Introduction: Diabetes mellitus (DM) has been recognized as the seventh leading cause of global mortality; however, researchers seek alternative means to manage the menace. The current study sought to investigate antioxidant potentials, α-amylase, and α-glucosidase inhibitory activities of ethanolic extract of Moringa oleifera flower in vitro. Methods: Antioxidant properties of the extract were appraised by assessing its inhibition against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (OH•), and hydrogen peroxide (H2O2) free radicals, as well as ferric reducing antioxidant power (FRAP), the antidiabetic activity was evaluated by α-amylase and α-glucosidase inhibition.Results: In this study, ethanolic extract of M. oleifera flower demonstrated a significant (P < 0.05) inhibition against DPPH free radical (43.57–83.56%) in a concentration-dependent manner, while FRAP (101.76 ± 1.63 mg/100 g), OH• scavenging ability (71.62 ± 0.95 mg/100 g), and H2O2 free radical scavenging capacity...

International Journal of Pharmacy and Chemistry, 2021

The binding property of five (5) commonly used binders in metronidazole granule and tablet formul... more The binding property of five (5) commonly used binders in metronidazole granule and tablet formulations was studied. The binders studied included gelatin, polyvinylpyrrolidone (PVP), carboxymethylcellulose (CMC), maize starch and guar gum. The binders were incorporated as 5%w/w of tablet weight in each case. The granules were formulated by the wet granulation technique while the tablets were prepared by compression. The properties of granules evaluated included flow rate, angle of repose, bulk and tapped densities, Hausner's ratio, Carr's index and moisture content. Tablet properties studied included weight uniformity, hardness, friability, thickness and diameter, disintegration time and dissolution rate. Results indicated that granules containing guar gum or PVP possessed flow properties superior to those of other binders, while those containing gelatin possessed the least flow property. In terms of packing characteristics, granules containing CMC were better than others. The mechanical strength of tablets containing gelatin, PVP or CMC was superior to those made with either guar gum or maize starch. In terms of release of metronidazole, tablets containing PVP, gelatin or guar gum were more efficient in releasing the drug, while those made with CMC was the least. On the basis of these, good metronidazole tablets could be formulated using PVP, gelatin or guar gum. On the other hand, maize starch or CMC may not be recommended as a binder for metronidazole tablets because of high friability or poor release respectively.

International Journal of Veterinary Science

Obesity-induced by a high-fat diet in animal studies recapitulates the human form of obesity. Obe... more Obesity-induced by a high-fat diet in animal studies recapitulates the human form of obesity. Obesity is a serious public health concern, a risk factor for many diseases, including cardiovascular disorders. Our study evaluated the impact of orlistat on the left ventricular myocardium in high fat diet-induced obese adult Wistar rats. Our study included fifteen adult male Wistar rats weighing between (130-150g). The rats following environmental acclimatization were assigned into three groups A-C (n=5 each), the control (standard rat diet), high-fat diet only, high-fat diet, and orlistat as A, B, and C, respectively. The rats were placed on diets for eight weeks, which performed the sacrifice and histological procedures. The heart and left ventricles were excised and fixed in 10% buffered formalin solution for histological studies. Body Mass Index (BMI) of the high-fat diet-only group markedly increased (P<0.05) relative to the control and the orlistat-treated groups. Heart weight a...