Seun Adebajo - Academia.edu (original) (raw)

Papers by Seun Adebajo

Journal of Natural Sciences Engineering and Technology, 2021

Statement of the Problem: Tuberculosis remains a serious public-health threat in developing count... more Statement of the Problem: Tuberculosis remains a serious public-health threat in developing countries though it has been eradicated in some advanced countries. This disease constitutes a significant threat to global health, being the second highest cause of morbidity and mortality resulting from infectious agents. Prompt diagnosis of active TB facilitates timely therapeutic intervention and minimizes community transmission. Aim: This study aimed at determining a ‘Point of Care’ diagnostic tool for pulmonary tuberculosis (PTB) by comparing the efficiency of four different PTB diagnostic tools for different age groups. Methodology: Zeihl Nelson (ZN) staining, culture, Gene xpert (GX) and Lipoarabinomanan (LAM) assay were employed in this study The culture method was used for confirmation. Sputum and urine samples were collected from each of 100 patients symptomatically diagnosed of PTB. Findings: Fifty-seven percent of the population was male while 43% were female. Mycobacterium tuber...

Nigerian Journal of Horticultural Science, 2020

Ife Journal of Science, 2018

Biosurfactants are amphiphilic compounds produced by a variety of microorganisms as extracellular... more Biosurfactants are amphiphilic compounds produced by a variety of microorganisms as extracellular compounds. Biosurfactants are preferable over the chemical surfactant because of their effectiveness, degradability and environmentally friendly nature. This study focuses on the isolation, identification and screening of biosurfactants-producing bacteria. Soil samples were collected from different automobile shops in Abeokuta, Ogun State. Bacteria were isolated and screened for their ability to produce biosurfactant using different methods in a stepwise process such as haemolytic test, drop collapse test, bacterial adhesion to hydrocarbon test and the confirmatory test which was the emulsification test that was assayed every 4 hours. Forty-one bacteria isolates showed beta-haemolysis, 20 exhibited alpha haemolysis and 47 exhibited gamma haemolysis. All positive isolates from the beta haemolytic test were screened for drop collapse test and result showed that 14 isolates were positive to the drop-collapse test. The isolates adhered to hydrocarbon at different rates. Proteus mirabilis had the lowest hydrophobicity value of 41% and the highest was Pseudomonas aeruginosa with a hydrophobicity value of 72%. The result showed that Pseudomonas aeruginosa had the maximum emulsification capacity of 65% at 24 hours while the least emulsification capability for biosurfactant production was exhibited by Proteus mirabilis (43%). This result showed that P. aeruginosa is a better organism for biosurfactant production and are good choice of organisms in many industries because of their emulsion capability.

Human activities have degenerated nearly 40% of the universe soil through excessive chemical inpu... more Human activities have degenerated nearly 40% of the universe soil through excessive chemical inputs, tilling and industrial activities. Biochar can increase soil fecundity, agricultural productivity and protects soil-borne diseases. This study aimed to assess the efficacy of rice husk biochar on the growth and yield of tomato plants. Biochar was produced through feedstock (rice-husk) pyrolysis and used as soil amendments at different concentrations (20 t/ha and 40 t/ha) and an unamend soil served as control. Physicochemical properties of the soil, rice husk, biochar and biochar soil before and after planting were determined using standard methods. Rhizospheric microflora of the plants were isolated and identified based on morphological and biochemical characterizations. Agronomic parameters such as plant height, stem girth, leaf area, number of leaves, flowers, fruits and weight of fruits were determined. Results revealed that rice husk biochar (40 t/ha) had the highest physical and...

Colloids and Surfaces B: Biointerfaces

Scientific Reports, 2022

Tomato is beneficial to human health because it contains valuable vitamins such as vitamins A, C ... more Tomato is beneficial to human health because it contains valuable vitamins such as vitamins A, C and several minerals. However, to meet up with the demands of the ever increasing population, there is need to improve tomato production. This research, thus, investigated the impact of rice-husk biochar on the agronomic performances of tomato plant and microbial biomass of carbon, nitrogen and phosphorus in different tomato growth stages. The rice husk biochar pyrolyzed at 350 °C was amended with soil at four different application rates: 0, 2.5, 5.0 and 7.5 t/ha. Physicochemical property of soil was conducted using Mid Infrared Reflectance Spectroscopy method. Impact of biochar on Microbial Biomass Carbon, Microbial Biomass Nitrogen and Microbial Biomass Phosphorous was conducted using fumigation extraction method and monitored at three functional stages. Biochar application appreciably increase the soil physicochemical properties such as pH, Ca, Na, H+, S, P, B, C, Zn and cation exchan...

Bulletin of the National Research Centre, 2021

Background Environmental deterioration arising from the misuse of pesticides and chemical fertili... more Background Environmental deterioration arising from the misuse of pesticides and chemical fertilizers in agriculture has resulted in the pursuit of eco-friendly means of growing crop. Evidence has shown that biofertilizers and biocontrol can boost soil fertility and suppress soil pathogens without compromising the safety of the environment. Hence, the study investigated the use of termitarium soil as a viable source for biofertilizer and biocontrol. Results Twenty-seven soil samples were collected from nine different mound soil (household, farm and water bodies in a sterile sample bag). Aliquots of serially diluted samples were plated on nutrient agar, plate count agar, eosin methylene blue agar and MacConkey agar plates. Isolates were identified using standard microbiological techniques. Identified isolates were screened for plant growth-promoting properties using phosphate solubilization test, potassium solubilization test and indole acetic acid production test. Activities of the ...

Journal of Natural Sciences Engineering and Technology, 2020

Biosurfactants are one of the microbial bioproducts that are naturally synthesized and are applic... more Biosurfactants are one of the microbial bioproducts that are naturally synthesized and are applicable for many industrial purposes. In this study, antibacterial, stability and antibiotic susceptibility of biosurfactant was evaluated. Biosurfactants produced from different substrates (groundnut cake, cassava flour waste, pome, cooking oil, engine oil, cassava waste water, molasses, cassava peel, potato) by Pseudomonas taenensis were evaluated for antibacterial activity using agar well diffusion method. Antibiotics susceptibility of Pseudomonas taenensis was carried out using different antibiotics (augmentin, ofloxacin, tetracyclin and ciprofloxacin, cotrimoxazole, pefloxacin, amoxylin, ceftriazone, nitrofuranton and gentamycin). The stability of the biosurfactant was evaluated by adjusting the biosurfactant to: pH (2, 4, 6, 8, 10 and 12) using 1M NaOH and 1M HCl, temperature (4, 30, 37, 55, 75 and 100 °C) and NaCl (0, 5, 10, 15, 20 and 25 %). Results showed that only biosurfactant pr...

Applied Environmental Research, 2019

Microbial-derived surface-active compounds (biosurfactants) have attracted attention due to their... more Microbial-derived surface-active compounds (biosurfactants) have attracted attention due to their low toxicity, cost-effectiveness, biodegradable nature and environment compatibility. Due to paucity of knowledge in the production of biosurfactant by microorganisms from other sources such as biochar-amended soil, the present study investigates the potential of rhizospheric bacteria isolated from biochar amended soil of okra plant in the production of biosurfactants using different recovery techniques. Rhizospheric bacteria were screened for biosurfactant production using Haemolytic, Oil spreading, Drop collapse, Methylene blue method, Bacterial adhesion to hydrocarbon and Emulsification activity. The biosurfactant was extracted using different extraction solvents (acid precipitation, ethyl acetate, acetone, dichloromethane and chloroform/methanol). Degradation of hydrocarbon (diesel) was determined spectrophotometrically. A total of twenty-three rhizospheric bacteria were isolated fr...

Egyptian Journal of Basic and Applied Sciences, 2020

Biochar is a stable solid material made from biomass via pyrolysis and can endure in soil for tho... more Biochar is a stable solid material made from biomass via pyrolysis and can endure in soil for thousands of years. The present study aimed to evaluate the potential of rhizospheric microorganisms in rice-husk and poultry waste biochar-amended soil for biosurfactant production. Rhizospheric microorganisms in biochar-amended soil were isolated and screened for biosurfactant production using different screening methods. Potential rhizospheric bacteria was used to produce biosurfactant using different recovery techniques and its hydrocarbon degrading efficiency was determined. A total of 41 rhizospheric bacteria were recovered from biochar-amended soil. Pseudomonas and Bacillus spp were the dominant isolates. Pseudomonas putida showed best biosurfactant potential isolates from the different screening results and was used to produce biosurfactant. Chloroform-methanol showed good recovery solvent for biosurfactant extraction. Chloroform/methanol (2:1) gave the highest quantity (3.15 ± 0.26...

Tomato is beneficial to human health because it contains valuable vitamins such as vitamins A, C ... more Tomato is beneficial to human health because it contains valuable vitamins such as vitamins A, C and several minerals. However, to meet up with the demands of the ever increasing population, there is need to improve tomato production. This research, investigated the impact of biochar derived from rice husk on agronomic performances of tomato plant. The rice husk biochar pyrolysed at 350 ºC was amended with soil at four different application rates: 0, 2.5, 5.0 and 7.5 t/ha. Physicochemical property of soil was conducted using Mid Infrared Reflectance Spectroscopy method. Impact of biochar on Microbial Biomass Carbon, Microbial Biomass Nitrogen and Microbial Biomass Phosphorous was conducted using fumigation extraction method and monitored at three functional stages. Biochar application appreciably increase the soil physicochemical properties such as pH, Ca, Na, H+, S, P, B, Zn and cation exchangeable capacity. Biochar amended soil significantly enhanced tomato height, fruit yields an...

Microbes and Infectious Diseases, 2020

Background: Carbapenem-resistant Enterobacteriaceae (CRE) pose a great threat to the public healt... more Background: Carbapenem-resistant Enterobacteriaceae (CRE) pose a great threat to the public health sector as a result of its high communicability, high morbidity and mortality rates. This study investigated the occurrence of carbapenem resistant Enterobacteriaceae and the prevalence of Klebsiella pneumoniae carbapenemase (KPC) producing Klebsiella pneumoniae as a mechanism of resistance. Method: One hundred and ten Enterobacteriaceae isolates were cultured from different samples of patients in Intensive Care Units of two hospitals. Using Clinical and Laboratory Standards Institute (CLSI) Guidelines of 2017. Carbapenemase production was determined phenotypically using Brilliance CRE agar, Carbapenem inactivation method and confirmed with Modified Hodge test (MHT). Modified Hodge test positive isolates were screened for KPC-producing Klebsiella pneumoniae using boronic acid-based inhibition test. Result: Phenotypic results revealed an occurrence of 10.9% (12/110) isolates for carbapenem resistant Enterobacteriaceae while KPC-producing Klebsiella pneumoniae prevalence was 3.6% contributing 33.3% to the burden of carbapenem production. The occurrence of carbapenem-resistant Enterobacteriaceae in this study was relatively high and KPC-Klebsiella pneumoniae had a significant contribution to the burden of CRE. Conclusion: Hence, a sinewy antibiotic stewardship is needed in this regard.

Access Microbiology, 2020

Applied Environmental Research, 2017

merging multiple opportunities for industrial production and environmental applications have focu... more merging multiple opportunities for industrial production and environmental applications have focused increasing research attention on biosurfactants in recent years. Hydrocarbon-polluted soils have proved a major source of biosurfactant-producing bacteria. In this study an alternative method of producing biosurfactants was established, based on pharmaceutical effluents. Pharmaceutical effluents are a rich source of complex organic compounds with potential as a substrate for micro-bial biosurfactant production. A successful biosurfactant screening assay is achieved when a com-bination of different methods are employed. Biosurfactant producing bacteria were isolated from pharmaceutical effluent and identified by biochemical methods. The best biosurfactant producer was identified by a molecular method. The biosurfactant screening techniques employed were drop collapse assay, haemolytic assay, oil spreading assay, bacteria adhesion to hydrocarbon assay and emulsification assay. Analysis...

Arabian Journal of Chemistry

The application and relevance of marine-derived fungi in the mycoremediation of environment pollu... more The application and relevance of marine-derived fungi in the mycoremediation of environment polluted with polycyclic aromatic hydrocarbons (PAHs) is promising whilst limiting environmental hazards. The present study investigated the fluoranthene degradation efficiency of marine-derived fungal co-culture, Aspergillus aculeatus (AA) and Mucor irregularis (MI) in batch processes (Plackett-Burman experiments) enhanced with the addition of surfactants and solidstate substrates. Further optimization studies done through fractional factorial design revealed that

Nigerian Journal of Biotechnology, 2020

Cucumber (Cucumis sativus L.) production is generally low in Nigeria due to continuous soil nutri... more Cucumber (Cucumis sativus L.) production is generally low in Nigeria due to continuous soil nutrient limitation and diseases. However, the persistence in the use of agrochemicals for cucumber production in Nigeria is associated with high cost and deleterious effects on man, animal and the environment. This study was conducted to investigate the potentials of indigenous Bacillus thuringiensis (Bt), a spore-forming bacterium known for its insecticidal properties in controlling Fusarium wilt of cucumber. Bacillus thuringiensis strains were isolated from soil samples collected from different farm sites in Abeokuta, Nigeria, and identified phenotypically and molecularly. The in-vitro antagonistic activity of B. thuringiensis strains on F. oxysporum f.sp. cucumerinum was evaluated by dual culture method, followed by pot experiment in the screen house. 16S rRNA gene sequencing was performed on the antagonistic B. thuringiensis to confirm Bt species. The results of the in-vitro antagonistic...

Archives of Phytopathology and Plant Protection

Abstract Incorporation of compost amendments into the soil is a promising strategy for the contro... more Abstract Incorporation of compost amendments into the soil is a promising strategy for the control of some soil-borne plant diseases. The aim of this study was to evaluate the effect of compost amendments from Tridax procumbens L. and cow dung on the microbial activity and agronomic traits of tomato plants infested with Ralstonia solanacearum. Tridax procumbens L. and cow dung were composted for 20 days using five different proportions. Physicochemical properties and microbial load of the composts were determined using the standard methods. Isolates were identified based on morphological, colonial and biochemical characterisations. Different compost concentrations were added to sterile soil with and without Ralstonia solanacearum after which tomato nurseries were transplanted. Agronomic parameters such as plant height, number of leaves, stem girth and leave area were determined after two and four weeks of transplanting. Cow dung only (CD) had the highest Total Bacterial Count (TBC) and Total Fungal Count (TFC) of 265.67 x 104 CFU/g and 92.3 x 103 CFU/g respectively while T. procumbens only (TP) had the least TBC (46.3 x 104 CFU/g) and TFC (13.33 x 103 CFU/g) . Bacillus species, Pseudomonas species, Aspergillus species and Penicillium sp. were predominant throughout the duration of composting. Highest pH (7.3) was recorded by Cow dung + T. procumbens (C:T)(1:1) while TP had the least pH (6.2). Highest temperature (41.9 °C) was recorded by C:T(1:2) while TP recorded the least 29.8 °C. After four weeks, the compost amendments improved the plant height, number of leaves, stem girth, and leaf area in the un-infested and diseased tomato plants. This study showed that compost from T. procumbens and cow dung can be used to improve the growth of tomato and also suppress bacterial wilt of tomato.

Journal of Natural Sciences Engineering and Technology, 2021

Statement of the Problem: Tuberculosis remains a serious public-health threat in developing count... more Statement of the Problem: Tuberculosis remains a serious public-health threat in developing countries though it has been eradicated in some advanced countries. This disease constitutes a significant threat to global health, being the second highest cause of morbidity and mortality resulting from infectious agents. Prompt diagnosis of active TB facilitates timely therapeutic intervention and minimizes community transmission. Aim: This study aimed at determining a ‘Point of Care’ diagnostic tool for pulmonary tuberculosis (PTB) by comparing the efficiency of four different PTB diagnostic tools for different age groups. Methodology: Zeihl Nelson (ZN) staining, culture, Gene xpert (GX) and Lipoarabinomanan (LAM) assay were employed in this study The culture method was used for confirmation. Sputum and urine samples were collected from each of 100 patients symptomatically diagnosed of PTB. Findings: Fifty-seven percent of the population was male while 43% were female. Mycobacterium tuber...

Nigerian Journal of Horticultural Science, 2020

Ife Journal of Science, 2018

Biosurfactants are amphiphilic compounds produced by a variety of microorganisms as extracellular... more Biosurfactants are amphiphilic compounds produced by a variety of microorganisms as extracellular compounds. Biosurfactants are preferable over the chemical surfactant because of their effectiveness, degradability and environmentally friendly nature. This study focuses on the isolation, identification and screening of biosurfactants-producing bacteria. Soil samples were collected from different automobile shops in Abeokuta, Ogun State. Bacteria were isolated and screened for their ability to produce biosurfactant using different methods in a stepwise process such as haemolytic test, drop collapse test, bacterial adhesion to hydrocarbon test and the confirmatory test which was the emulsification test that was assayed every 4 hours. Forty-one bacteria isolates showed beta-haemolysis, 20 exhibited alpha haemolysis and 47 exhibited gamma haemolysis. All positive isolates from the beta haemolytic test were screened for drop collapse test and result showed that 14 isolates were positive to the drop-collapse test. The isolates adhered to hydrocarbon at different rates. Proteus mirabilis had the lowest hydrophobicity value of 41% and the highest was Pseudomonas aeruginosa with a hydrophobicity value of 72%. The result showed that Pseudomonas aeruginosa had the maximum emulsification capacity of 65% at 24 hours while the least emulsification capability for biosurfactant production was exhibited by Proteus mirabilis (43%). This result showed that P. aeruginosa is a better organism for biosurfactant production and are good choice of organisms in many industries because of their emulsion capability.

Human activities have degenerated nearly 40% of the universe soil through excessive chemical inpu... more Human activities have degenerated nearly 40% of the universe soil through excessive chemical inputs, tilling and industrial activities. Biochar can increase soil fecundity, agricultural productivity and protects soil-borne diseases. This study aimed to assess the efficacy of rice husk biochar on the growth and yield of tomato plants. Biochar was produced through feedstock (rice-husk) pyrolysis and used as soil amendments at different concentrations (20 t/ha and 40 t/ha) and an unamend soil served as control. Physicochemical properties of the soil, rice husk, biochar and biochar soil before and after planting were determined using standard methods. Rhizospheric microflora of the plants were isolated and identified based on morphological and biochemical characterizations. Agronomic parameters such as plant height, stem girth, leaf area, number of leaves, flowers, fruits and weight of fruits were determined. Results revealed that rice husk biochar (40 t/ha) had the highest physical and...

Colloids and Surfaces B: Biointerfaces

Scientific Reports, 2022

Tomato is beneficial to human health because it contains valuable vitamins such as vitamins A, C ... more Tomato is beneficial to human health because it contains valuable vitamins such as vitamins A, C and several minerals. However, to meet up with the demands of the ever increasing population, there is need to improve tomato production. This research, thus, investigated the impact of rice-husk biochar on the agronomic performances of tomato plant and microbial biomass of carbon, nitrogen and phosphorus in different tomato growth stages. The rice husk biochar pyrolyzed at 350 °C was amended with soil at four different application rates: 0, 2.5, 5.0 and 7.5 t/ha. Physicochemical property of soil was conducted using Mid Infrared Reflectance Spectroscopy method. Impact of biochar on Microbial Biomass Carbon, Microbial Biomass Nitrogen and Microbial Biomass Phosphorous was conducted using fumigation extraction method and monitored at three functional stages. Biochar application appreciably increase the soil physicochemical properties such as pH, Ca, Na, H+, S, P, B, C, Zn and cation exchan...

Bulletin of the National Research Centre, 2021

Background Environmental deterioration arising from the misuse of pesticides and chemical fertili... more Background Environmental deterioration arising from the misuse of pesticides and chemical fertilizers in agriculture has resulted in the pursuit of eco-friendly means of growing crop. Evidence has shown that biofertilizers and biocontrol can boost soil fertility and suppress soil pathogens without compromising the safety of the environment. Hence, the study investigated the use of termitarium soil as a viable source for biofertilizer and biocontrol. Results Twenty-seven soil samples were collected from nine different mound soil (household, farm and water bodies in a sterile sample bag). Aliquots of serially diluted samples were plated on nutrient agar, plate count agar, eosin methylene blue agar and MacConkey agar plates. Isolates were identified using standard microbiological techniques. Identified isolates were screened for plant growth-promoting properties using phosphate solubilization test, potassium solubilization test and indole acetic acid production test. Activities of the ...

Journal of Natural Sciences Engineering and Technology, 2020

Biosurfactants are one of the microbial bioproducts that are naturally synthesized and are applic... more Biosurfactants are one of the microbial bioproducts that are naturally synthesized and are applicable for many industrial purposes. In this study, antibacterial, stability and antibiotic susceptibility of biosurfactant was evaluated. Biosurfactants produced from different substrates (groundnut cake, cassava flour waste, pome, cooking oil, engine oil, cassava waste water, molasses, cassava peel, potato) by Pseudomonas taenensis were evaluated for antibacterial activity using agar well diffusion method. Antibiotics susceptibility of Pseudomonas taenensis was carried out using different antibiotics (augmentin, ofloxacin, tetracyclin and ciprofloxacin, cotrimoxazole, pefloxacin, amoxylin, ceftriazone, nitrofuranton and gentamycin). The stability of the biosurfactant was evaluated by adjusting the biosurfactant to: pH (2, 4, 6, 8, 10 and 12) using 1M NaOH and 1M HCl, temperature (4, 30, 37, 55, 75 and 100 °C) and NaCl (0, 5, 10, 15, 20 and 25 %). Results showed that only biosurfactant pr...

Applied Environmental Research, 2019

Microbial-derived surface-active compounds (biosurfactants) have attracted attention due to their... more Microbial-derived surface-active compounds (biosurfactants) have attracted attention due to their low toxicity, cost-effectiveness, biodegradable nature and environment compatibility. Due to paucity of knowledge in the production of biosurfactant by microorganisms from other sources such as biochar-amended soil, the present study investigates the potential of rhizospheric bacteria isolated from biochar amended soil of okra plant in the production of biosurfactants using different recovery techniques. Rhizospheric bacteria were screened for biosurfactant production using Haemolytic, Oil spreading, Drop collapse, Methylene blue method, Bacterial adhesion to hydrocarbon and Emulsification activity. The biosurfactant was extracted using different extraction solvents (acid precipitation, ethyl acetate, acetone, dichloromethane and chloroform/methanol). Degradation of hydrocarbon (diesel) was determined spectrophotometrically. A total of twenty-three rhizospheric bacteria were isolated fr...

Egyptian Journal of Basic and Applied Sciences, 2020

Biochar is a stable solid material made from biomass via pyrolysis and can endure in soil for tho... more Biochar is a stable solid material made from biomass via pyrolysis and can endure in soil for thousands of years. The present study aimed to evaluate the potential of rhizospheric microorganisms in rice-husk and poultry waste biochar-amended soil for biosurfactant production. Rhizospheric microorganisms in biochar-amended soil were isolated and screened for biosurfactant production using different screening methods. Potential rhizospheric bacteria was used to produce biosurfactant using different recovery techniques and its hydrocarbon degrading efficiency was determined. A total of 41 rhizospheric bacteria were recovered from biochar-amended soil. Pseudomonas and Bacillus spp were the dominant isolates. Pseudomonas putida showed best biosurfactant potential isolates from the different screening results and was used to produce biosurfactant. Chloroform-methanol showed good recovery solvent for biosurfactant extraction. Chloroform/methanol (2:1) gave the highest quantity (3.15 ± 0.26...

Tomato is beneficial to human health because it contains valuable vitamins such as vitamins A, C ... more Tomato is beneficial to human health because it contains valuable vitamins such as vitamins A, C and several minerals. However, to meet up with the demands of the ever increasing population, there is need to improve tomato production. This research, investigated the impact of biochar derived from rice husk on agronomic performances of tomato plant. The rice husk biochar pyrolysed at 350 ºC was amended with soil at four different application rates: 0, 2.5, 5.0 and 7.5 t/ha. Physicochemical property of soil was conducted using Mid Infrared Reflectance Spectroscopy method. Impact of biochar on Microbial Biomass Carbon, Microbial Biomass Nitrogen and Microbial Biomass Phosphorous was conducted using fumigation extraction method and monitored at three functional stages. Biochar application appreciably increase the soil physicochemical properties such as pH, Ca, Na, H+, S, P, B, Zn and cation exchangeable capacity. Biochar amended soil significantly enhanced tomato height, fruit yields an...

Microbes and Infectious Diseases, 2020

Background: Carbapenem-resistant Enterobacteriaceae (CRE) pose a great threat to the public healt... more Background: Carbapenem-resistant Enterobacteriaceae (CRE) pose a great threat to the public health sector as a result of its high communicability, high morbidity and mortality rates. This study investigated the occurrence of carbapenem resistant Enterobacteriaceae and the prevalence of Klebsiella pneumoniae carbapenemase (KPC) producing Klebsiella pneumoniae as a mechanism of resistance. Method: One hundred and ten Enterobacteriaceae isolates were cultured from different samples of patients in Intensive Care Units of two hospitals. Using Clinical and Laboratory Standards Institute (CLSI) Guidelines of 2017. Carbapenemase production was determined phenotypically using Brilliance CRE agar, Carbapenem inactivation method and confirmed with Modified Hodge test (MHT). Modified Hodge test positive isolates were screened for KPC-producing Klebsiella pneumoniae using boronic acid-based inhibition test. Result: Phenotypic results revealed an occurrence of 10.9% (12/110) isolates for carbapenem resistant Enterobacteriaceae while KPC-producing Klebsiella pneumoniae prevalence was 3.6% contributing 33.3% to the burden of carbapenem production. The occurrence of carbapenem-resistant Enterobacteriaceae in this study was relatively high and KPC-Klebsiella pneumoniae had a significant contribution to the burden of CRE. Conclusion: Hence, a sinewy antibiotic stewardship is needed in this regard.

Access Microbiology, 2020

Applied Environmental Research, 2017

merging multiple opportunities for industrial production and environmental applications have focu... more merging multiple opportunities for industrial production and environmental applications have focused increasing research attention on biosurfactants in recent years. Hydrocarbon-polluted soils have proved a major source of biosurfactant-producing bacteria. In this study an alternative method of producing biosurfactants was established, based on pharmaceutical effluents. Pharmaceutical effluents are a rich source of complex organic compounds with potential as a substrate for micro-bial biosurfactant production. A successful biosurfactant screening assay is achieved when a com-bination of different methods are employed. Biosurfactant producing bacteria were isolated from pharmaceutical effluent and identified by biochemical methods. The best biosurfactant producer was identified by a molecular method. The biosurfactant screening techniques employed were drop collapse assay, haemolytic assay, oil spreading assay, bacteria adhesion to hydrocarbon assay and emulsification assay. Analysis...

Arabian Journal of Chemistry

The application and relevance of marine-derived fungi in the mycoremediation of environment pollu... more The application and relevance of marine-derived fungi in the mycoremediation of environment polluted with polycyclic aromatic hydrocarbons (PAHs) is promising whilst limiting environmental hazards. The present study investigated the fluoranthene degradation efficiency of marine-derived fungal co-culture, Aspergillus aculeatus (AA) and Mucor irregularis (MI) in batch processes (Plackett-Burman experiments) enhanced with the addition of surfactants and solidstate substrates. Further optimization studies done through fractional factorial design revealed that

Nigerian Journal of Biotechnology, 2020

Cucumber (Cucumis sativus L.) production is generally low in Nigeria due to continuous soil nutri... more Cucumber (Cucumis sativus L.) production is generally low in Nigeria due to continuous soil nutrient limitation and diseases. However, the persistence in the use of agrochemicals for cucumber production in Nigeria is associated with high cost and deleterious effects on man, animal and the environment. This study was conducted to investigate the potentials of indigenous Bacillus thuringiensis (Bt), a spore-forming bacterium known for its insecticidal properties in controlling Fusarium wilt of cucumber. Bacillus thuringiensis strains were isolated from soil samples collected from different farm sites in Abeokuta, Nigeria, and identified phenotypically and molecularly. The in-vitro antagonistic activity of B. thuringiensis strains on F. oxysporum f.sp. cucumerinum was evaluated by dual culture method, followed by pot experiment in the screen house. 16S rRNA gene sequencing was performed on the antagonistic B. thuringiensis to confirm Bt species. The results of the in-vitro antagonistic...

Archives of Phytopathology and Plant Protection

Abstract Incorporation of compost amendments into the soil is a promising strategy for the contro... more Abstract Incorporation of compost amendments into the soil is a promising strategy for the control of some soil-borne plant diseases. The aim of this study was to evaluate the effect of compost amendments from Tridax procumbens L. and cow dung on the microbial activity and agronomic traits of tomato plants infested with Ralstonia solanacearum. Tridax procumbens L. and cow dung were composted for 20 days using five different proportions. Physicochemical properties and microbial load of the composts were determined using the standard methods. Isolates were identified based on morphological, colonial and biochemical characterisations. Different compost concentrations were added to sterile soil with and without Ralstonia solanacearum after which tomato nurseries were transplanted. Agronomic parameters such as plant height, number of leaves, stem girth and leave area were determined after two and four weeks of transplanting. Cow dung only (CD) had the highest Total Bacterial Count (TBC) and Total Fungal Count (TFC) of 265.67 x 104 CFU/g and 92.3 x 103 CFU/g respectively while T. procumbens only (TP) had the least TBC (46.3 x 104 CFU/g) and TFC (13.33 x 103 CFU/g) . Bacillus species, Pseudomonas species, Aspergillus species and Penicillium sp. were predominant throughout the duration of composting. Highest pH (7.3) was recorded by Cow dung + T. procumbens (C:T)(1:1) while TP had the least pH (6.2). Highest temperature (41.9 °C) was recorded by C:T(1:2) while TP recorded the least 29.8 °C. After four weeks, the compost amendments improved the plant height, number of leaves, stem girth, and leaf area in the un-infested and diseased tomato plants. This study showed that compost from T. procumbens and cow dung can be used to improve the growth of tomato and also suppress bacterial wilt of tomato.