Eman Sharaf - Academia.edu (original) (raw)

Papers by Eman Sharaf

Journal of International Environmental Application and Science, 2012

Three haloalkaliphilic bacteria were isolated from alkaline (pH 8.84) and hyper saline soil (TSS ... more Three haloalkaliphilic bacteria were isolated from alkaline (pH 8.84) and hyper saline soil (TSS 19 mS/cm) collected from Al- Malileh at the north of Al- Madinah Al-Munawrah. These were Bacillus circulans1, B. sphaericus and B. brevis. Qualitative screening and quantitative estimation for protease production by the isolates revealed that B. circulans1 was the most potent producer followed by B. sphaericus and B. brevis. The maximum protease production was observed after inoculating the culture broth (pH 8.5) with B.circulans1 inoculums of 48 h age and incubated under shaking at45°C for 48 h. Presence of 0.5% sucrose, 1% NH 4 NO 3 and 10%NaCl in the growth medium achieved maximum proteas production.

Egyptian journal of microbiology, 1991

Research Journal of Microbiology, Dec 1, 2011

Egyptian Journal of Botany, 2010

Acta microbiologica Polonica, 1999

Infectio, 2016

Shiga toxin-producing Escherichia coli (STEC) strains have emerged as important foodborne pathoge... more Shiga toxin-producing Escherichia coli (STEC) strains have emerged as important foodborne pathogens of global public health concern, causing life-threatening diseases. Sheep and their products have been documented as important reservoirs for STECs, especially E. coli O157. The aim of this study was to investigate STECs from diarrheal human and sheep in Al-Madinah Al-Munawarah, Saudi Arabia. Fecal samples were collected between June and August, 2015 from diarrheal humans (n = 134) and sheep (n = 87). Presumptive E. coli humanand sheep-isolated strains were identified for their serotypes, the associated virulence genes (Shiga toxin [stx 1 , stx 2 ], haemolysin [ehxA] and intimin [eae]) by polymerase chain reaction and their susceptibility to antibiotics. Pulsed-field gel electrophoresis (PFGE) was used to demonstrate the genetic relatedness between Serotype O157:H7 human-and sheep-isolated strains. Forty eight (48/221; 21.7%) STECs were recovered from both human and sheep, their serotypes were as follows:

African Journal of Microbiology Research, Sep 6, 2013

Saudi journal of biological sciences, 2011

Sodium dodecyl sulfate-polyacrlyamide gel electrophoresis (SDS-PAGE) was used to assess the purit... more Sodium dodecyl sulfate-polyacrlyamide gel electrophoresis (SDS-PAGE) was used to assess the purity and molecular weight of the previously purified alkaline keratinase enzyme of Scopulariopsis brevicaulis. The enzyme was homogenous, as seen by a single band of protein, and had an apparent molecular weight of 28.5 kDa. Amino acid profile of the purified keratinase revealed that it was composed of 14 different amino acids with high proportions of glutamic acid (20.86%), alanine (14.52%), glycine (14.21%), leucine (8.59%) and serine (7.81%). The enzyme contained moderate amounts of valine (6.01%), threonine (5.58%) and phenyl alanine (5.22%). The purified enzyme of S. brevicaulis exerted a potent keratinolytic activity and was capable to hydrolyze different keratinaceous materials with highest activity on chicken feathers followed by human nails and human hair.

Polish journal of microbiology, 2004

The phytohormone IAA (indol-3-acetic acid) was tested in vitro on growth of tomato wilt pathogen ... more The phytohormone IAA (indol-3-acetic acid) was tested in vitro on growth of tomato wilt pathogen Fusarium oxysporum lycopersici. The hormone reduced spore germination, mycelial dry weight and protein content. Such reduction was matched with the elevation in the hormone concentration. The in vivo application of IAA to soil of the uninoculated plants (controls) improved growth and yielded longer shoot and root, particularly at low concentrations. Moreover, the hormone could prevent completely any chance for disease incidence by soil pathogens. Presence of IAA in soil of inoculated plants not only reduced the infection rate but also increased plant growth, causing that they appeared healthy and normal. Disease suppression in tomato plants, exerted by application of IAA, was achieved through either increasing plant growth, exerting a direct harmful effect on the target pathogen and/or inducing resistance in host tissue. The induced resistance was correlated with induction of certain sec...

a Department of Biology, Faculty of Science, Taibah University, Al Madinah Al Munawarah, Saudi Ar... more a Department of Biology, Faculty of Science, Taibah University, Al Madinah Al Munawarah, Saudi Arabia b Department of Botany and Microbiology,Faculty of Science, Cairo University,Giza,Egypt. c Faculty of Veterinary Medicine, Department of Bacteriology, Immunology and Mycology, Suez Canal University, Egypt * Autor para correspondencia. Correo electrónico: emanfsharaf@yahoo.com (E.F. Sharaf). Recibido: 08/02/2016; Aceptado: 20/05/2016

Fungal infection is one of the greatest problems facing storage of foods. Early in 1969, Christen... more Fungal infection is one of the greatest problems facing storage of foods. Early in 1969, Christensen and Kaufman approved that seeds are subject for fungal contamination during storage which may destroy or at least devaluate them. Droby (2006) and Zhu (2006) estimated that about 20 up to 25% of fruits are damaged due to post-harvest handling and storage. Fungal infections of fruits may occur during growing, harvesting, handling, transport and storage (Bamidele et al., 2012). Furthermore, some of these fungi produce serious carcinogenic mycotoxins (Smith and Ross, 1991). One of these toxins is aflatoxins which are produced by certain species of Aspergillus like A. flavus and A. parasiticus (Palmgren and Ciegler, 1983; Samson and Frisvad, 1990; Trucksess et al., 2002). Accordingly, destruction of International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 5 Number 11 (2016) pp. 691-702 Journal homepage: http://www.ijcmas.com

The aim of the present study is to assess the effect of aqueous and ethanol cinnamon extract on s... more The aim of the present study is to assess the effect of aqueous and ethanol cinnamon extract on some growth criteria of the opportunistic mold Aspergillus fumigatus I 2. Ethanol extract or hot water extract of cinnamon were prepared and mixed with Aspergillus fumigatus I 2 spore suspension and calculations were performed for count and percentage of the germinated spores. Various concentrations of ethanol cinnamon extract were performed to test their effect on fungal linear growth on Czapek Dox's agar medium and dry weight, protein and polysaccharide content in Czapek Dox's liquid medium. The results showed that Cinnamon ethanol extract was effective as it completely inhibited the germination of spores for 48 hours while the aqueous one has a fungistatic effect which reduced with an increase in the incubation period. There was significant effect of cinnamon ethanol extracts (different concentration) on both A. fumigatus I 2 linear growth and dry weight that reduced, notably when concentration increases. The presence of the cinnamon extract in A. fumigatus I 2 growth medium is found to decrease the mycelium polysaccharide and protein contents. GC-Mass spectrometry also showed the presence of cinnamaldehyde in the cinnamon ethanol and aqueous extracts alongside benzoic acid, benzaldehyde, and volatile oil. The test revealed an increasing concentration of the active ingredients with the ethanol extract highlighting its substantial antifungal activities.

Indian Journal of Pharmaceutical Sciences

International Journal of Current Microbiology and Applied Sciences, 2016

African Journal of Microbiology Research, 2013

Journal of Biological Sciences

Bioremediation Journal, 2015

The accuracy of the Content should not be relied upon and should be independently verified with p... more The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden.

Polish journal of microbiology / Polskie Towarzystwo Mikrobiologów = The Polish Society of Microbiologists, 2005

Eight fungal species characterized by chitinolytic activity were isolated from Egyptian black san... more Eight fungal species characterized by chitinolytic activity were isolated from Egyptian black sand collected from Rosetta coast. Genus Aspergillus and Alternaria alternata exhibited the highest density (> 40% of the total count, each) on the isolation plates containing different treatments of native shrimp shell chitin. Genus Aspergillus was represented by A. flavus, A. niger, A. foetidus and A. tungius, with the former species being the most dominant. The other species were Cladosporium herbarum, Fusarium equisitum (5.71% of the total count, each) and Dendryphiella vinosa (3.21% of the total count). The isolated species were screened for chitinase production on agar plates containing 0.2% colloidal chitin. The chitinolytic activity of each individual was not always correlated with its density on the isolation plates. Alternaria alternata was the most promising species for chitinase excretion. The use of colloidal chitin (1.5%) as a sole carbon source was superior for the enzyme ...

Polish journal of microbiology / Polskie Towarzystwo Mikrobiologów = The Polish Society of Microbiologists, 2004