The use of cinnamon aqueous and ethanolic extracts in controlling some growth criteria of the opportunistic mold Aspergillus fumigatus I2 (original) (raw)
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Aspergillus flavus growth response to cinnamon extract and sodium benzoate mixtures
Food Control, 2007
The effects of selected combinations of cinnamon extract (CE, 0, 50, 100, 200, 400 or 800 ppm) and sodium benzoate (NaB, 0, 12.5, 25, 50, 100, 200, 400 or 800 ppm) on the growth response of Aspergillus flavus inoculated on potato-dextrose agar (PDA) adjusted to 0.98 a w and pH 3.5 or 4.5 were evaluated for 30 days. Minimal inhibitory concentrations (MIC) were determined, transformed into fractional inhibitory concentrations (FIC) and a FIC index was computed. Cinnamon extract MIC was 200 ppm and not affected by pH, whereas for NaB a pH reduction from 4.5 to 3.5 reduced the MIC from 800 to 400 ppm. At pH 3.5 additive mixtures included 200 ppm of NaB, whereas at pH 4.5 these mixtures exhibited a synergic effect (FIC index = 0.75). Mixtures of CE and NaB are promising antifungal agents.
2020
The current study carried out to isolate and identify fungal species producing mycotoxins from dried figs and the possibility of reducing their presence in fruits and reducing their toxic effects using Cinnamon extract. The isolation results showed contamination of local and imported dried figs with stored fungi, especially A. niger and A. flavus. It was found that the frequency of appearance rate of A. niger on the local (Iraqi) and imported (Iranian, Syrian, Turkish) dried figs was 60.72.72.16.66 and 75%, while the appearance of occurrences rate was 100, 100, 20 and 60%, respectively. The results of chemical analysis of TLC showed that 19 out of the 26 tested isolates of A. niger were produced Ochratoxin A with a production rate of 73.07%. The results also showed that different concentrations of Cinnamon extract had high efficacy in inhibition of A. niger where the inhibition of fungal growth was increased with increasing Cinnamon extract concentration, with the highest inhibition...
In Vivo Study on the Hindrance Activity of Cinnamon Extract Against Aspergillus niger in Mice
Aim: The antimycotic effect and immune stimulating capacity of natural extract of Cinnamomum zeylanicum plant was evaluated against Aspergillus niger strain. Methods and results: the herbal extract was prepared from cinnamon park to be examined against Aspergillus niger (ATCC16404)species fungal cell suspension. Mice were injected with both fungal cell suspensions and Cinnamomum zeylanicum extract in a certain regime. Histopathological examination was applied on lung, liver and brain tissues extracted from the experimental animals and histopathological findings revealed the strong fungicidal effect of the herbal extract in the mice's tissues. Phagocytic activity, interleukins 2 & 6 (IL 2& IL6) and tumor necrosis factor(TNF) were measured in mice's blood samples as immunity stimulating efficacy parameters and also results revealed the potent immune stimulating efficacy of the extract and its safetyin vivo. Conclusion: According to the results of the study, Cinnamomum zeylanicum extract has a strong fungicidal activity against A. niger(ATCC16404)in addition to potent immune stimulating action confirmed experimentally. Results are supporting the efficacy of C. zeylanicum extract as prophylactic agent as well as fungicide. Significance and impact of the study: our study spotted a light on the advantages of the examined herbal extract as antimycotic substance, the promising results revealed encourages the use of C. zeylanicum extract as pharmaceutical preparation for treating and prevention of mycosis in immunocompromised patients instead of using some chemical antimycotic preparations available for commercial use to avoid the disadvantages of these chemically prepared medications.
African Journal of Agricultural Research, 2018
Among the fungi that cause damage and/or are spread by seeds, Aspergillus sp. and Sclerotinia sclerotiorum stand out, which have a worldwide distribution and a wide range of hosts. A viable and safer option than chemicals would be to use natural compounds for plant disease management. The objective of this study was to evaluate cinnamon (Cinnamomum cassia) and citronella (Cymbopogon winterianus) essential oils in the in vitro control of fungi Aspergilus sp. and S. sclerotiorum. The experimental design was completely randomized in a 2x4 + 2 factorial scheme [essential oils x concentrations + (fungicide + standard control)]. Cinnamon and citronella essential oils were used in doses of 0.2, 0.4, 0.8 and 1.6 mL L-1 (+Tween 80 to 1%) and the Captana (480 g L-1) and thiophanatemethyl + chlorothalonil (200.0 g kg-1 + 500.0 g kg-1) fungicides, at doses of 3 g L-1 and 2 g L-1 , for the fungi Aspergilus sp. and S. sclerotiorum, respectively. The products were diluted in potato dextrose agar (PDA) medium in Petri dishes, and mycelium discs with 5 mm diameter were placed and incubated in a Biochemical Oxygen Demand (BOD) incubator at 25 ± 1°C and photoperiod of 12 h. There was significant interaction between treatments. The dose of 1.6 mL L-1 of both oils showed greater inhibition of the mycelial growth of fungi Aspergilus sp. and S. sclerotiorum, and the greater inhibition of sporulation of the fungus Aspergilus sp. It is concluded that cinnamon and citronella essential oils control the fungi Aspergilus sp. and S. sclerotiorum.
INHIBITORY EFFECT OF ESSENTIAL OILS ON THE GROWTH OF Aspergillus flavus
2007
The effects of 16 essential oils from aromatic plants were tested for their inhibitory effect on Aspergillus flavus IMI 242684 on PDA. The results showed that the essential oil of white wood (Melaleuca cajeputi) gave the highest inhibition followed by the essential oils of cinnamon (Cinnamomum cassia) and lavender (Lavandula officinalis), respectively. Furthermore, the inhibitory effects of these three essential oils at different concentrations were examined. It was found that the essential oil of white wood at 1.5625% (v/v) and of cinnamon and lavender at 50% (v/v) were the optimum concentrations for fungal growth inhibition. The essential oil of white wood at 25% (v/v) completely inhibited the growth of A. flavus IMI 242684 on PDA for 28 days.
Journal of Pure and Applied Microbiology, 2021
Aspergillus flavus is a frequent contaminant of maize grain. We isolated this fungus, determined the colony morphology and species (by internal transcribed spacer sequencing) and measured the aflatoxin content. The selected A. flavus fungi were placed into two groups, toxigenic and atoxigenic; both appeared similar morphologically, except that the atoxigenic group lacked sclerotia. An essential oil fumigation test with clove and cinnamon oils as antifungal products was performed on fungal conidial discs and fungal colonies in Petri plates. Cinnamon oil at 2.5 to 5.0 μL/plate markedly inhibited the mycelial growth from conidial discs of both strains, whereas clove oil showed less activity. The oils had different effects on fungal mycelia. The higher clove fumigation doses of 10.0 to 20.0 μL/plate controlled fungal growth, while cinnamon oil caused less inhibition. Compared with atoxigenic groups, toxigenic A. flavus responded stably. Within abnormal A. flavus hyphae, the essential oi...
Influence of essential oils on the growth of aspergillus flavus
Potravinarstvo Slovak Journal of Food Sciences, 2017
This paper was focused on the determination of the inhibitory effect of selected essential oils on growth of ten isolates of Aspergillus flavus and their potential ability to produce mycotoxins in vitro by TLC method. The isolates were obtained from moldy bread of domestic origin. We followed the impact of five essential oils at 100% concentration - lemon, eucalyptus, oregano, sage and thyme. The effect of the essential oils we tested the gaseous diffusion method. We isolates grown on CYA (Czapek yeast extract agar), in the dark at 25 ±1 °C, 14 days. The diameter of colonies grown we continuously measured on the 3rd, 7th, 11th, and 14th day of cultivation. The results of the paper suggest that oregano and thyme essential oil had 100% inhibited the growth of all tested isolates of Aspergillus flavus. Lemon, eucalyptus and sage essential oil had not significant inhibitory effects on tested isolates Aspergillus flavus, but affected the growth of colonies throughout the cultivation. In ...
In vitro antifungal effect of cinnamon extract on candida species
Caspian journal of dental research, 2018
Introduction: Cinnamon zeylanicum is used for various medical purposes. The aim of this study was to compare the antifungal efficacy of cinnamon and amphotericin B against Candida species in vitro. Material & Methods: Candida albicans (C. albicans) and Candida krusei (C. Krusei) were obtained from the Iranian Industrial and Scientific Research Center. The minimum inhibitory concentration (MIC) of cinnamon extract was determined and compared with that of amphotericin B. Results: The results of this study showed that Amphotericin B, ethanolic and aqueous extract of cinnamon inhibited the growth of C. albicans and C. Krusei with different MICs. Conclusion: Ethanolic extract of cinnamon has inhibitory effects on Candida species comparable to that of amphotericin B. So, it can be used as a herbal alternative.
Molecules
The effect of essential oils (obtained using hydrodistillation) and plant extracts (ethanolic, aqueous, and hexanic extractions) of 10 different plants cultivated in Brazil were tested using the diffusion agar method, with the objective of evaluating the inhibitory effect of the oils and extracts on the mycelial growth of Aspergillus westerdijkiae NRRL 3174 and A. carbonarius RC 2054 (UNRC). Of the 40 essential oils and plant extracts analyzed, oregano essential oil and plant extract, rosemary essential oil, and the clove ethanolic extract were the best choice to obtain the growth parameters (radial growth rates (mm day−1) and lag phase (h)) due the good results presented and the volume of oil/extract obtained. Comparing all the essential oils and plant extracts that were tested for growth parameters, the best results were obtained for the clove ethanolic extract for both strains assayed. These results demonstrated an outstanding potential use of some of these products in prevention...