Sheila Sait - Academia.edu (original) (raw)

Papers by Sheila Sait

Research paper thumbnail of Mouse and Human Homologues of the Yeast Origin of Replication Recognition Complex Subunit ORC2 and Chromosomal Localization of the Cognate Human Gene ORC2L

Research paper thumbnail of Recurrent involvement of 11q13 in acute nonlymphocytic leukemia

Cancer genetics and cytogenetics, Jun 1, 1987

Research paper thumbnail of The kinase insert domain receptor gene (KDR) has been relocated to chromosome 4q11-->q12

Cytogenetic and Genome Research, 1995

Research paper thumbnail of Delay to formalin fixation effect on breast biomarkers

Modern Pathology, Nov 1, 2009

Research paper thumbnail of Langerhans cell sarcoma arising from chronic lymphocytic lymphoma/small lymphocytic leukemia: lineage analysis and braf v600e mutation study

North American Journal of Medical Sciences, 2013

Research paper thumbnail of Uniparentalism in sporadic colorectal cancer is independent of imprint status, and coordinate for chromosomes 14 and 18

Cancer genetics and cytogenetics, Mar 1, 2009

Research paper thumbnail of Physical and transcript mapping in the vicinity of chromosome breakpoints associated with Beckwith-Wiedemann Syndrome and a rhabdoid tumor

American Journal of Human Genetics, Sep 1, 1994

ABSTRACT

Research paper thumbnail of Langerhans Cell Sarcoma Arising from Small Lymphocytic Lymphoma: Lineage Analysis and BRAF V600E Mutation study

American Journal of Clinical Pathology, Sep 1, 2013

Research paper thumbnail of Cytogenetic study of maturing granulocytes in bone marrow of patients with acute myelogenous leukemia

PubMed, Apr 1, 1990

To determine the cytogenetic origin of maturing granulocytes in the bone marrow of patients with ... more To determine the cytogenetic origin of maturing granulocytes in the bone marrow of patients with acute myelogenous leukemia, bone marrow cells were studied using a modified cytogenetic technique, which does not disrupt the cell membrane, in conjunction with periodic acid-Schiff (PAS) staining. In four cases successfully studied, myeloblasts were PAS-negative and granulocytes were PAS-positive. In three cases successfully studied following 0-2 days of culture, metaphase spreads with abnormal karyotypes characteristic of the patients' leukemic clones were seen in five of five, six of nine, and four of four PAS-positive cells successfully studied. These patients' bone marrows were AN, AA, and AA, respectively, by standard cytogenetic study. Therefore, the cytogenetic status of PAS-positive cells did not necessarily correlate with presence or absence of normal metaphases determined by standard cytogenetic study. Bone marrow cells which underwent full and partial granulocytic maturation in suspension culture were studied following 2 weeks of culture. Abnormal karyotypes were seen in five of five and two of two metaphases in PAS-positive cells successfully studied in two patients. Therefore, we have demonstrated that when acute myelogenous leukemia cells undergo myeloid maturation in culture, the mature cells may be definitely proven to derive from leukemic progenitors rather than from normal stem cells.

Research paper thumbnail of A uterine leiomyoma showing both t(12;14) and del(7) abnormalities

Cancer genetics and cytogenetics, Feb 1, 1989

The involvement of chromosomes 12 and 14 in uterine leiomyomas has been well established. However... more The involvement of chromosomes 12 and 14 in uterine leiomyomas has been well established. However, in a recen! report of only a del(7)(q22.1q31.32) or (q l l.2q22.3) in two cases of typical uterine leiomyoma, Boghosian et al. hypothesized that this could represent a cytagenetic subgroup of uterine leiomyomas. We report a case cff uterine leiomyoma with both the t(12;14) and del(7) in all the cells examined and discuss the implications af this in terms of critical chromosomal rearrangements underlying the route to benign cellular proliferation.

Research paper thumbnail of Concomitant Venetoclax and Imatinib for Comanaging Chronic Lymphocytic Leukemia and Chronic Myeloid Leukemia: A Case Report

Journal of The National Comprehensive Cancer Network, Feb 1, 2023

Research paper thumbnail of Knock-Down of Signal Transducer and Activator of Transcription 3 Enhances Acute Myeloid Leukemia-Derived Dendritic Cell Function

Blood, Nov 20, 2009

Abstract 1972 Poster Board I-995 The only successful immunologic approach in acute myeloid leukem... more Abstract 1972 Poster Board I-995 The only successful immunologic approach in acute myeloid leukemia (AML) is allogeneic transplantation. However, this treatment modality is restricted by age and donor availability. It is also associated with significant toxicity. Therefore, autologous approaches are being sought. Among those, harnessing the…

Research paper thumbnail of Three possible cytogenetic subgroups of leiomyosarcoma

Cancer genetics and cytogenetics, Nov 1, 1989

Research paper thumbnail of Letter to the editor: Translocation (7;21)(p14;q11) in Ph-positive chronic myelogenous leukemia

Cancer genetics and cytogenetics, Jul 1, 1988

Research paper thumbnail of A t(1;11) in acute nonlymphocytic leukemia FAB type M4

Cancer genetics and cytogenetics, 1987

Abnormalities involving the long arm of chromosome #11 have been shown to be involved in a high p... more Abnormalities involving the long arm of chromosome #11 have been shown to be involved in a high proportion of acute nonlymphocytic leukemias, specifically FAB types M4 and M5. In particular, band 11q23 seems to be preferentially affected. Reported herein is a case of acute nonlymphocytic leukemia type M4 showing a t(1;11)(q21;q23).

Research paper thumbnail of Consistent chromosome changes in leiomyosarcoma

Cancer genetics and cytogenetics, Oct 1, 1988

Karyotypic analysis of a leiomyosarcoma of the retroperitoneum revealed some structural and numer... more Karyotypic analysis of a leiomyosarcoma of the retroperitoneum revealed some structural and numerical changes. Review of the literature showed that some of these changes, namely involvement of 1p13 and 11p13 and monosomy 9, 18 and 22 seemed to occur frequently. These changes could be characteristic of leiomyosarcoma and define a cytogenetic subgroup within this tumor entity.

Research paper thumbnail of Isochromosome 1q in a myelodysplastic syndrome after treatment for acute promyelocytic leukemia

Cancer genetics and cytogenetics, Jun 1, 2006

A growing body of literature reports therapy-related myelodysplastic syndrome (t-MDS) and acute m... more A growing body of literature reports therapy-related myelodysplastic syndrome (t-MDS) and acute myeloid leukemia (t-AML) in patients treated successfully for acute promyelocytic leukemia (APL). We report a t-MDS with an isochromosome 1q as a sole abnormality, 47,XY,+1,i(1)(q10), in a 46-year-old man with APL 14 years after he was treated with cytosine arabinosine and daunorubicin. The literature on t-MDS/t-AML after APL therapy and on isochromosome 1q is reviewed.

Research paper thumbnail of Chromosome changes in metastatic human melanoma

Cancer genetics and cytogenetics, Feb 1, 1988

Cytogenetic studies were performed on human malignant melanoma cells from eight metastatic lesion... more Cytogenetic studies were performed on human malignant melanoma cells from eight metastatic lesions. Five tumors displayed near-triploid and three near-diploid chromosome numbers. Chromosomes #1, #6, #7, followed by #2 and #9, were found to be most frequently involved in structural aberrations. Aberrations involving chromosome #1, with deletions or translocations of 1p, involving region 1p12-1p22 in seven of eight breakpoints of the p arm were observed. Seven of nine breakpoints of 6q were located at region 6q15-6q21. Most of the breakpoints on chromosome #7 occurred near the centromeric region. All tumors had additional chromosome material involving 1q, chromosome #7 (7q in two tumors), and in five tumors an increased dose of chromosome #6 (6p in one tumor). The nonrandom breakpoints of these and other chromosomes involved diverse bands, including loci of oncogenes and fragile sites. The observation of nonrandom chromosomal changes in advanced malignant melanoma suggests that genes important in the progression of melanoma are located on chromosomes #1, #6, and #7.

Research paper thumbnail of Chromosomes in adipose tissue tumors. An evaluation of 75 cases

Cancer genetics and cytogenetics, Sep 1, 1989

Research paper thumbnail of Involvement of 6p in benign lipomas

Cancer genetics and cytogenetics, Feb 1, 1989

Research paper thumbnail of Mouse and Human Homologues of the Yeast Origin of Replication Recognition Complex Subunit ORC2 and Chromosomal Localization of the Cognate Human Gene ORC2L

Research paper thumbnail of Recurrent involvement of 11q13 in acute nonlymphocytic leukemia

Cancer genetics and cytogenetics, Jun 1, 1987

Research paper thumbnail of The kinase insert domain receptor gene (KDR) has been relocated to chromosome 4q11-->q12

Cytogenetic and Genome Research, 1995

Research paper thumbnail of Delay to formalin fixation effect on breast biomarkers

Modern Pathology, Nov 1, 2009

Research paper thumbnail of Langerhans cell sarcoma arising from chronic lymphocytic lymphoma/small lymphocytic leukemia: lineage analysis and braf v600e mutation study

North American Journal of Medical Sciences, 2013

Research paper thumbnail of Uniparentalism in sporadic colorectal cancer is independent of imprint status, and coordinate for chromosomes 14 and 18

Cancer genetics and cytogenetics, Mar 1, 2009

Research paper thumbnail of Physical and transcript mapping in the vicinity of chromosome breakpoints associated with Beckwith-Wiedemann Syndrome and a rhabdoid tumor

American Journal of Human Genetics, Sep 1, 1994

ABSTRACT

Research paper thumbnail of Langerhans Cell Sarcoma Arising from Small Lymphocytic Lymphoma: Lineage Analysis and BRAF V600E Mutation study

American Journal of Clinical Pathology, Sep 1, 2013

Research paper thumbnail of Cytogenetic study of maturing granulocytes in bone marrow of patients with acute myelogenous leukemia

PubMed, Apr 1, 1990

To determine the cytogenetic origin of maturing granulocytes in the bone marrow of patients with ... more To determine the cytogenetic origin of maturing granulocytes in the bone marrow of patients with acute myelogenous leukemia, bone marrow cells were studied using a modified cytogenetic technique, which does not disrupt the cell membrane, in conjunction with periodic acid-Schiff (PAS) staining. In four cases successfully studied, myeloblasts were PAS-negative and granulocytes were PAS-positive. In three cases successfully studied following 0-2 days of culture, metaphase spreads with abnormal karyotypes characteristic of the patients' leukemic clones were seen in five of five, six of nine, and four of four PAS-positive cells successfully studied. These patients' bone marrows were AN, AA, and AA, respectively, by standard cytogenetic study. Therefore, the cytogenetic status of PAS-positive cells did not necessarily correlate with presence or absence of normal metaphases determined by standard cytogenetic study. Bone marrow cells which underwent full and partial granulocytic maturation in suspension culture were studied following 2 weeks of culture. Abnormal karyotypes were seen in five of five and two of two metaphases in PAS-positive cells successfully studied in two patients. Therefore, we have demonstrated that when acute myelogenous leukemia cells undergo myeloid maturation in culture, the mature cells may be definitely proven to derive from leukemic progenitors rather than from normal stem cells.

Research paper thumbnail of A uterine leiomyoma showing both t(12;14) and del(7) abnormalities

Cancer genetics and cytogenetics, Feb 1, 1989

The involvement of chromosomes 12 and 14 in uterine leiomyomas has been well established. However... more The involvement of chromosomes 12 and 14 in uterine leiomyomas has been well established. However, in a recen! report of only a del(7)(q22.1q31.32) or (q l l.2q22.3) in two cases of typical uterine leiomyoma, Boghosian et al. hypothesized that this could represent a cytagenetic subgroup of uterine leiomyomas. We report a case cff uterine leiomyoma with both the t(12;14) and del(7) in all the cells examined and discuss the implications af this in terms of critical chromosomal rearrangements underlying the route to benign cellular proliferation.

Research paper thumbnail of Concomitant Venetoclax and Imatinib for Comanaging Chronic Lymphocytic Leukemia and Chronic Myeloid Leukemia: A Case Report

Journal of The National Comprehensive Cancer Network, Feb 1, 2023

Research paper thumbnail of Knock-Down of Signal Transducer and Activator of Transcription 3 Enhances Acute Myeloid Leukemia-Derived Dendritic Cell Function

Blood, Nov 20, 2009

Abstract 1972 Poster Board I-995 The only successful immunologic approach in acute myeloid leukem... more Abstract 1972 Poster Board I-995 The only successful immunologic approach in acute myeloid leukemia (AML) is allogeneic transplantation. However, this treatment modality is restricted by age and donor availability. It is also associated with significant toxicity. Therefore, autologous approaches are being sought. Among those, harnessing the…

Research paper thumbnail of Three possible cytogenetic subgroups of leiomyosarcoma

Cancer genetics and cytogenetics, Nov 1, 1989

Research paper thumbnail of Letter to the editor: Translocation (7;21)(p14;q11) in Ph-positive chronic myelogenous leukemia

Cancer genetics and cytogenetics, Jul 1, 1988

Research paper thumbnail of A t(1;11) in acute nonlymphocytic leukemia FAB type M4

Cancer genetics and cytogenetics, 1987

Abnormalities involving the long arm of chromosome #11 have been shown to be involved in a high p... more Abnormalities involving the long arm of chromosome #11 have been shown to be involved in a high proportion of acute nonlymphocytic leukemias, specifically FAB types M4 and M5. In particular, band 11q23 seems to be preferentially affected. Reported herein is a case of acute nonlymphocytic leukemia type M4 showing a t(1;11)(q21;q23).

Research paper thumbnail of Consistent chromosome changes in leiomyosarcoma

Cancer genetics and cytogenetics, Oct 1, 1988

Karyotypic analysis of a leiomyosarcoma of the retroperitoneum revealed some structural and numer... more Karyotypic analysis of a leiomyosarcoma of the retroperitoneum revealed some structural and numerical changes. Review of the literature showed that some of these changes, namely involvement of 1p13 and 11p13 and monosomy 9, 18 and 22 seemed to occur frequently. These changes could be characteristic of leiomyosarcoma and define a cytogenetic subgroup within this tumor entity.

Research paper thumbnail of Isochromosome 1q in a myelodysplastic syndrome after treatment for acute promyelocytic leukemia

Cancer genetics and cytogenetics, Jun 1, 2006

A growing body of literature reports therapy-related myelodysplastic syndrome (t-MDS) and acute m... more A growing body of literature reports therapy-related myelodysplastic syndrome (t-MDS) and acute myeloid leukemia (t-AML) in patients treated successfully for acute promyelocytic leukemia (APL). We report a t-MDS with an isochromosome 1q as a sole abnormality, 47,XY,+1,i(1)(q10), in a 46-year-old man with APL 14 years after he was treated with cytosine arabinosine and daunorubicin. The literature on t-MDS/t-AML after APL therapy and on isochromosome 1q is reviewed.

Research paper thumbnail of Chromosome changes in metastatic human melanoma

Cancer genetics and cytogenetics, Feb 1, 1988

Cytogenetic studies were performed on human malignant melanoma cells from eight metastatic lesion... more Cytogenetic studies were performed on human malignant melanoma cells from eight metastatic lesions. Five tumors displayed near-triploid and three near-diploid chromosome numbers. Chromosomes #1, #6, #7, followed by #2 and #9, were found to be most frequently involved in structural aberrations. Aberrations involving chromosome #1, with deletions or translocations of 1p, involving region 1p12-1p22 in seven of eight breakpoints of the p arm were observed. Seven of nine breakpoints of 6q were located at region 6q15-6q21. Most of the breakpoints on chromosome #7 occurred near the centromeric region. All tumors had additional chromosome material involving 1q, chromosome #7 (7q in two tumors), and in five tumors an increased dose of chromosome #6 (6p in one tumor). The nonrandom breakpoints of these and other chromosomes involved diverse bands, including loci of oncogenes and fragile sites. The observation of nonrandom chromosomal changes in advanced malignant melanoma suggests that genes important in the progression of melanoma are located on chromosomes #1, #6, and #7.

Research paper thumbnail of Chromosomes in adipose tissue tumors. An evaluation of 75 cases

Cancer genetics and cytogenetics, Sep 1, 1989

Research paper thumbnail of Involvement of 6p in benign lipomas

Cancer genetics and cytogenetics, Feb 1, 1989