Shivaprakash Hiremath - Academia.edu (original) (raw)

Papers by Shivaprakash Hiremath

Shodhganga, Dec 31, 1995

This is to certify that the thesis entitled " REPRODUCTIVE PATTERN OF THE SEA CUCUMBER Holothuria... more This is to certify that the thesis entitled " REPRODUCTIVE PATTERN OF THE SEA CUCUMBER Holothuria atra Jaeger (ECHINODERMATA : HOLOTHUROIDEA) OF Shri.S.V.HIREMATH for the award of the Degree of Doctor of Philosophy in Zoology is based on the results of the experiments carried out by him in our Departments under our supervision. The thesis or a part thereof has not been previously presented for any Diploma or Degree.

Asian Journal of Medical Sciences, Aug 31, 2016

Background: Oral Submucous Fibrosis is one of the oral potentially malignant disorders presenting... more Background: Oral Submucous Fibrosis is one of the oral potentially malignant disorders presenting with progressive restriction in mouth opening. The condition having a high malignant transformation rate necessitates identifi cation of biomarkers to be employed for early detection of malignant change. This will infl uence the prognosis in addition to adding better quality of life to patients. Aims and Objectives: To relate the association of VEGF-460C/T polymorphism in patients with OSMF and to compare the same among healthy subjects. Materials and Methods: Thirty patients with Oral submucous fi brosis and 20 controls free from habits and any form of lesions were included in the study. The polymorphism of VEGF gene was detected by polymerase chain reaction-based restriction analysis. Results: Sixty-four percent of the population in the study was in the age group of 21-40 yrs suggesting the prominence of disease in younger individuals with male predominance. With reference to polymorphism, 6.67% of the subjects from OSMF group showed CT polymorphism and 16.67% showed TT polymorphism. There were no statistically signifi cant differences in the polymorphism between the study group and controls. However the frequency of T allele in the patient group 12 (20%) was greater than that in the control group 1 (2.5%), which was a signifi cant fi nding. There was no association between the habits, frequency of habits, duration of quid placement, site of quid placement and style of chewing with the nature of polymorphism. Conclusion: VEGF 460C/T has the potential to be used as a prognostic marker in predicting the malignant transformation of OSMF.

International Journal of Case Reports and Images, 2012

Introduction: OralFacialDigital (OFD) syndrome is the collective name of a group of rare inherite... more Introduction: OralFacialDigital (OFD) syndrome is the collective name of a group of rare inherited syndromes characterised by malformations of the mouth, face, hands and feet. Currently, nine OFD syndromes have been identified and among them OFD I and OFD II are comparatively common. These two can be distinguished from each other with the help of skeletal Xrays and by the observation of various specific symptoms and also have different patterns of inheritance. Case Report: A case report of a female child with features of OFD II also called as Mohr syndrome is presented in this study. Comparison with all the previously determined features of Mohr syndrome was done with this case and investigations were carried. Conclusion: The report showed the confirmation of Mohr Syndrome in this child along with some uncommon features like congenital heart disease.

This is to certify that the thesis entitled " REPRODUCTIVE PATTERN OF THE SEA CUCUMBER Holothuria... more This is to certify that the thesis entitled " REPRODUCTIVE PATTERN OF THE SEA CUCUMBER Holothuria atra Jaeger (ECHINODERMATA : HOLOTHUROIDEA) OF Shri.S.V.HIREMATH for the award of the Degree of Doctor of Philosophy in Zoology is based on the results of the experiments carried out by him in our Departments under our supervision. The thesis or a part thereof has not been previously presented for any Diploma or Degree.

Cervical Cancer (CC) accounts as one of the major gynecological malignancy among women, Human Pap... more Cervical Cancer (CC) accounts as one of the major gynecological malignancy among women, Human Papilloma Virus (HPV) being a prime etiological factor for causing cervical cancer. Hence, it is necessary to detect the prevalence of HPV in this population. This study was conducted to detect the prevalence of HPV 16 and 18 in North Karnataka population by the latest molecular tool Polymerase Chain Method. Between 2010-2012 women within the age group between 29-86 years visiting the hospital with chief gynecological complaints were considered for the study. A total of one hundred and thirty four samples were collected and out of these one hundred and twenty two samples were subjected to a molecular technique polymerase chain reaction using consensus primer at E6 region for HPV types 16 and 18.One hundred and one samples were positive for HPV 16 (82.78%) and ninety two were HPV 18 (75.40 %) and eighty (65.57%) were with 16/18.Chi Square test was performed and the findings showed significan...

Applied Cancer Research, 2017

Background: OSF is a potentially malignant condition affecting the oral cavity and oropharynx. MM... more Background: OSF is a potentially malignant condition affecting the oral cavity and oropharynx. MMP-3 also known as Stromelysin-I is a key member of the MMP family which is responsible for degradation of collagen type II,IV,V,IX and X, proteoglycans, gelatins, fibronectin, laminin and elastin. It plays an important role in activation of pro MMP-1 into the active form of MMP-1 in malignant tissues. MMP-3 expression is low in normal tissues but it is altered during tumour formation, where remodeling of ECM is required. Purpose of the study: To assess the association of single-nucleotide polymorphisms, Adenosine (Insertion/ Deletion) in-1171 5A > 6A in the MMP-3 promoter regions of patients with oral submucous fibrosis and in healthy individuals (controls). Methods: Thirty cases of OSF were categorized according to Khanna et al classification into four groups and Twenty age and sex matched controls were included in the study. Blood samples were collected in EDTA coated vacutainers and PCR restriction analysis was done. A statistical analysis was done using Chi-square test and Fisher's exact test to assess the frequency and association of the alleles in the case-control group. Results: The result showed a statistical significance difference between the duration of habit and disease severity with polymorphisms. The result also showed a higher frequency of the 5A allele in the study group as compared to the controls. Conclusion: A long-term follow up of these patients is mandatory to see the prognosis and their susceptibility to malignancy. The positive outcome of an association of the disease with polymorphisms would result in the development of potential diagnostic and therapeutic possibilities in potentially malignant and malignant lesions.

International Journal of Case Reports and Images, 2012

Introduction: OralFacialDigital (OFD) syndrome is the collective name of a group of rare inherite... more Introduction: OralFacialDigital (OFD) syndrome is the collective name of a group of rare inherited syndromes characterised by malformations of the mouth, face, hands and feet. Currently, nine OFD syndromes have been identified and among them OFD I and OFD II are comparatively common. These two can be distinguished from each other with the help of skeletal Xrays and by the observation of various specific symptoms and also have different patterns of inheritance. Case Report: A case report of a female child with features of OFD II also called as Mohr syndrome is presented in this study. Comparison with all the previously determined features of Mohr syndrome was done with this case and investigations were carried. Conclusion: The report showed the confirmation of Mohr Syndrome in this child along with some uncommon features like congenital heart disease.

Asian Journal of Medical Sciences, 2011

Objective: Familial Hypercholesterolemia (FH) is a metabolic disorder inherited as an autosomal d... more Objective: Familial Hypercholesterolemia (FH) is a metabolic disorder inherited as an autosomal dominant trait characterized by an increased plasma low-density lipoprotein (LDL) level. The disease is caused by several different mutations in the LDL receptor (LDLR) gene. Several mutations have been reported in this gene in patients from several ethnic groups. Early identification of individuals carrying the defective gene could be useful in reducing the risk of atherosclerosis and myocardial infraction by the available therapeutic methods. The techniques available for determining the number of the functional LDLR molecules are difficult to carry out and expensive. Our study presents mutation analysis of the LDLR gene in 24 Indian families with FH. Material & Methods: Peripheral blood samples were obtained form individuals after taking informed consent on the condition that each of these individuals had at least one first-degree relative affected with FH. Genomic DNA was isolated, exo...

Journal of Applied Biology & Biotechnology, 2018

The young shoots of Bambusa bambos are used ayurvedic medicines in India. Young shoots content va... more The young shoots of Bambusa bambos are used ayurvedic medicines in India. Young shoots content various chemical components such as cholin, betain, urease, cyanogenetic glucosides, oxalic acid, and benzoic acid. It has antiulcer, antifertility, anti-inflammatory, and antioxidant functions. In the present study, the hepatoprotective activity of methanolic shoot extract of B. bambos was tested against carbon tetrachloride (CCl 4)-induced hepatotoxicity in rats. Female Wistar rats were divided into five groups; Group I served as normal control. Groups II-VI were administered CCl4 mixed with olive oil 1:1 intraperitoneally (1 mL/kg body weight), after every 72 h for 16 days. Group II was CCl 4 negative control. Groups IV and V received methanolic shoot extract mixed with olive oil, 200 mg and 400 mg/ kg body weight, respectively. Group III received silymarin 50 mg/kg body weight for 16 days orally once daily. Methanolic extract attenuated the increase in aspartate amino transaminase (AST) and alanine amino transaminase (ALT) as well as alkaline phosphatase (ALP) and total bilirubin that occur during liver injury after CCl 4 injection. Outcome of the present study suggests that treatment with methanolic shoot extract of B. bambos-induced reduction in ALT, AST, ALP, and total bilirubin in rats indicating hepatoprotective potential of the extract.

Indian Journal of Radiology and Imaging, 2019

Objectives: To highlight the typical magnetic resonance imaging (MRI) findings in hyperglycemia-i... more Objectives: To highlight the typical magnetic resonance imaging (MRI) findings in hyperglycemia-induced seizures and compare the results with similar previous studies with a brief mention of pathophysiological mechanisms. Materials and Methods: This retrospective study included medical and imaging records of six consecutive patients with hyperglycemia-induced seizures. The data analysis included a clinical presentation and biochemical parameters at admission. The MRI sequences were evaluated for region involved, presence of subcortical T2 hypo-intensity, cortical hyper-intensity, and restricted diffusion. Similar previous studies from the National Library of Medicine (NLM) were analyzed and compared with our study. Results: Twenty-four patients were included from four studies in previous literature for comparison. In our study, on imaging, posterior cerebral region was predominantly involved, with parietal involvement in 83.3%, followed by occipital, frontal, and temporal involvemen...

The Indian journal of radiology & imaging

The purpose of our study was to assess the prevalence of variations in the extent of sphenoid pne... more The purpose of our study was to assess the prevalence of variations in the extent of sphenoid pneumatization in the Indian population and compare with existing literature. This retrospective study included 500 patients who underwent CT of the paranasal sinuses. The multiplanar reformations of paranasal sinus were assessed for the type of pneumatization of the sphenoid sinus and type of clival, lateral recess, lesser wing, and anterior recess extensions. The conchal, presellar, incomplete sellar, and complete sellar types comprised 0%, 1.2%, 22.2%, and 76.6% of patients. The extensions of pneumatization subtypes in the study population were clival in 76.6% subjects; lateral recess, lesser wing, and anterior recess in 59.7%, 20.4%, and 20.4% of sinuses, respectively. The pure forms were seen in 25.4% and combined forms in 61% of sinuses. The presellar type (1.2%) was less common and sellar type (98.8%) being common in our population compared to the Caucasian and East Asian population....

Polish Journal of Radiology, 2017

Background: Mid-esophageal region is an uncommon location of esophageal diverticula, a condition ... more Background: Mid-esophageal region is an uncommon location of esophageal diverticula, a condition usually diagnosed in elderly individuals.

Journal of neuroradiology. Journal de neuroradiologie, Jan 8, 2017

To assess the significance of association and possible correlation between hemorrhage, cytotoxic ... more To assess the significance of association and possible correlation between hemorrhage, cytotoxic edema, blood pressure and imaging severity in posterior reversible encephalopathy syndrome (PRES). This retrospective study included the medical and imaging records of 35 consecutive patients with PRES. The clinical data analysis included the highest recorded blood pressure (BP) on the day of the ictus, MRI including spin-echo echo planar diffusion-weighted imaging (DWI), susceptibility weighted angiography (SWAN) and conventional sequences. The presence of hemorrhage and cytotoxic edema was evaluated for the significance of correlation and association with each other and with blood pressure and imaging severity. On MR imaging, hemorrhage was found in 25.7%, and cytotoxic edema in 20% of patients. There was no statistically significant association of hemorrhage (P=0.403) and cytotoxic edema (P=0.162) with BP in contrast to significant association of hemorrhage (P<0.001) and cytotoxic ...

Oral Lichen planus (OLP) is a disease of adulthood, and children are rarely affected. It may invo... more Oral Lichen planus (OLP) is a disease of adulthood, and children are rarely affected. It may involve various mucosal surfaces either independently or concurrently (oral, skin, and oral and skin lesions). Prevalence of skin LP in general population is 0.9% -1.2% and prevalence of oral LP is reported between 0.1% and 2.2% (1). The relative risk for oral lichen planus was highest (13.7) among those who smoked and chewed tobacco . The etiopathogenesis appears to be complex, with interactions between genetic, environmental, lifestyle factors, and interestingly with new associations such as with liver disease have emerged. Viral infections have recently been linked with OLP. Herpes Simplex virus-1 (HSV-1), Cytomegalovirus (CMV), Human Herpes virus-6 (HHV-6), EpsteinBarr virus (EBV), Human Papilloma virus (HPV) and Hepatitis C virus (HCV) are virus types that have been studied in the etiopathogenesis of OLP(2). EBV is associated with infectious mononucleosis, oral hairy leukoplakia (nonmal...

The current investigation was focused on the assessment of antiproliferative effects of the Solan... more The current investigation was focused on the assessment of antiproliferative effects of the Solanum macranthum aqueous fruits extract against the human breast cancer cell line MDA-MB-231 in vitro. Phytochemical analysis of the S. macranthum aqueous fruits extract was performed and the phytoconstituents found positive for alkaloids, phenols, and saponins. Quantitative analysis of the total alkaloid and phenol was determined using gravimetric analysis and folins-phenols reagent method, respectively. The total alkaloid present was estimated to be 7.58% and total phenol of 158.774 mg/Gallic Acid Equivalent. The cytotoxicity of S. macranthum aqueous fruits extract was screened using 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay and camptothecin was used as standard reference drug. At the concentration of 500 μg/ml, the aqueous extract potentially inhibited the growth of the MDA-MB-231 cell line in vitro reaching 19%, and the IC50 value was calculated to be 525...

Journal of Oral and Maxillofacial Pathology : JOMFP, 2019

Introduction: The tumor-suppressor p53 protein is inactivated by the human papillomavirus (HPV) E... more Introduction: The tumor-suppressor p53 protein is inactivated by the human papillomavirus (HPV) E6 oncoprotein, causing polymorphism of the p53 at codon 72 of exon either proline (Pro) or arginine (Arg). Specific allele predisposition has been reported in the literature. The association between the p53 allele and HPV types has been reported. We analyzed the association between p53 polymorphism at codon 72 and HPV 16 and 18 genotypes in control, oral submucous fibrosis (OSF) and oral squamous cell carcinoma (OSCC). Materials and Methods: Of the total 90 cases, biopsy tissues of all groups (30 cases of OSF, OSCC and control each) were collected to extract DNA. Polymerase chain reaction was used to detect HPV 16 and 18 and alleles of codon 72 in p53 were evaluated in all the samples. Results: In control, OSF and OSCC samples showed the presence HPV 63.3%, 33.3% and 60%, respectively. In OSF, HPV 16 and 18 was detected in four and four cases, respectively, whereas in OSCC, HPV 16 and 18...

Clinical Phytoscience, 2020

Background The current study undertaken to evaluate antioxidant property of Solanum macranthum fr... more Background The current study undertaken to evaluate antioxidant property of Solanum macranthum fruit methanol extract and its in-vitro cytotoxic effects on breast cancer MDA-MB-231 and mice embryo fibroblast (MEF-L929) cell line. Methods The total alkaloids, phenols and flavonoids content in the methanol extract of S.macranthum fruit were estimated. The antioxidant activity was evaluated by FRAP assay, H2O2 scavenging assay and DPPH assay. Anti-inflammatory protein denaturation inhibition assay was carried out using bovine serum albumin. Anticancer property of the extract was evaluated against breast cancer MDA-MB-231 by MTT assay and comparative study was carried out on normal cells using MEF-L929 cell line. Results Phytochemical estimation revealed that total alkaloid content was measurable high (13.6 g/100 g of extract), where as the total phenolics and flavonoids content was 115.5 mg/GAE and 142.6 mg/QE. Further, the antioxidant effect of methanol extract by H2O2 assay showed co...

Asian Journal of Medical Sciences, 2016

Background: Oral Submucous Fibrosis is one of the oral potentially malignant disorders presenting... more Background: Oral Submucous Fibrosis is one of the oral potentially malignant disorders presenting with progressive restriction in mouth opening. The condition having a high malignant transformation rate necessitates identifi cation of biomarkers to be employed for early detection of malignant change. This will infl uence the prognosis in addition to adding better quality of life to patients. Aims and Objectives: To relate the association of VEGF-460C/T polymorphism in patients with OSMF and to compare the same among healthy subjects. Materials and Methods: Thirty patients with Oral submucous fi brosis and 20 controls free from habits and any form of lesions were included in the study. The polymorphism of VEGF gene was detected by polymerase chain reaction-based restriction analysis. Results: Sixty-four percent of the population in the study was in the age group of 21-40 yrs suggesting the prominence of disease in younger individuals with male predominance. With reference to polymorphism, 6.67% of the subjects from OSMF group showed CT polymorphism and 16.67% showed TT polymorphism. There were no statistically signifi cant differences in the polymorphism between the study group and controls. However the frequency of T allele in the patient group 12 (20%) was greater than that in the control group 1 (2.5%), which was a signifi cant fi nding. There was no association between the habits, frequency of habits, duration of quid placement, site of quid placement and style of chewing with the nature of polymorphism. Conclusion: VEGF 460C/T has the potential to be used as a prognostic marker in predicting the malignant transformation of OSMF.

Shodhganga, Dec 31, 1995

This is to certify that the thesis entitled " REPRODUCTIVE PATTERN OF THE SEA CUCUMBER Holothuria... more This is to certify that the thesis entitled " REPRODUCTIVE PATTERN OF THE SEA CUCUMBER Holothuria atra Jaeger (ECHINODERMATA : HOLOTHUROIDEA) OF Shri.S.V.HIREMATH for the award of the Degree of Doctor of Philosophy in Zoology is based on the results of the experiments carried out by him in our Departments under our supervision. The thesis or a part thereof has not been previously presented for any Diploma or Degree.

Asian Journal of Medical Sciences, Aug 31, 2016

Background: Oral Submucous Fibrosis is one of the oral potentially malignant disorders presenting... more Background: Oral Submucous Fibrosis is one of the oral potentially malignant disorders presenting with progressive restriction in mouth opening. The condition having a high malignant transformation rate necessitates identifi cation of biomarkers to be employed for early detection of malignant change. This will infl uence the prognosis in addition to adding better quality of life to patients. Aims and Objectives: To relate the association of VEGF-460C/T polymorphism in patients with OSMF and to compare the same among healthy subjects. Materials and Methods: Thirty patients with Oral submucous fi brosis and 20 controls free from habits and any form of lesions were included in the study. The polymorphism of VEGF gene was detected by polymerase chain reaction-based restriction analysis. Results: Sixty-four percent of the population in the study was in the age group of 21-40 yrs suggesting the prominence of disease in younger individuals with male predominance. With reference to polymorphism, 6.67% of the subjects from OSMF group showed CT polymorphism and 16.67% showed TT polymorphism. There were no statistically signifi cant differences in the polymorphism between the study group and controls. However the frequency of T allele in the patient group 12 (20%) was greater than that in the control group 1 (2.5%), which was a signifi cant fi nding. There was no association between the habits, frequency of habits, duration of quid placement, site of quid placement and style of chewing with the nature of polymorphism. Conclusion: VEGF 460C/T has the potential to be used as a prognostic marker in predicting the malignant transformation of OSMF.

International Journal of Case Reports and Images, 2012

Introduction: OralFacialDigital (OFD) syndrome is the collective name of a group of rare inherite... more Introduction: OralFacialDigital (OFD) syndrome is the collective name of a group of rare inherited syndromes characterised by malformations of the mouth, face, hands and feet. Currently, nine OFD syndromes have been identified and among them OFD I and OFD II are comparatively common. These two can be distinguished from each other with the help of skeletal Xrays and by the observation of various specific symptoms and also have different patterns of inheritance. Case Report: A case report of a female child with features of OFD II also called as Mohr syndrome is presented in this study. Comparison with all the previously determined features of Mohr syndrome was done with this case and investigations were carried. Conclusion: The report showed the confirmation of Mohr Syndrome in this child along with some uncommon features like congenital heart disease.

This is to certify that the thesis entitled " REPRODUCTIVE PATTERN OF THE SEA CUCUMBER Holothuria... more This is to certify that the thesis entitled " REPRODUCTIVE PATTERN OF THE SEA CUCUMBER Holothuria atra Jaeger (ECHINODERMATA : HOLOTHUROIDEA) OF Shri.S.V.HIREMATH for the award of the Degree of Doctor of Philosophy in Zoology is based on the results of the experiments carried out by him in our Departments under our supervision. The thesis or a part thereof has not been previously presented for any Diploma or Degree.

Cervical Cancer (CC) accounts as one of the major gynecological malignancy among women, Human Pap... more Cervical Cancer (CC) accounts as one of the major gynecological malignancy among women, Human Papilloma Virus (HPV) being a prime etiological factor for causing cervical cancer. Hence, it is necessary to detect the prevalence of HPV in this population. This study was conducted to detect the prevalence of HPV 16 and 18 in North Karnataka population by the latest molecular tool Polymerase Chain Method. Between 2010-2012 women within the age group between 29-86 years visiting the hospital with chief gynecological complaints were considered for the study. A total of one hundred and thirty four samples were collected and out of these one hundred and twenty two samples were subjected to a molecular technique polymerase chain reaction using consensus primer at E6 region for HPV types 16 and 18.One hundred and one samples were positive for HPV 16 (82.78%) and ninety two were HPV 18 (75.40 %) and eighty (65.57%) were with 16/18.Chi Square test was performed and the findings showed significan...

Applied Cancer Research, 2017

Background: OSF is a potentially malignant condition affecting the oral cavity and oropharynx. MM... more Background: OSF is a potentially malignant condition affecting the oral cavity and oropharynx. MMP-3 also known as Stromelysin-I is a key member of the MMP family which is responsible for degradation of collagen type II,IV,V,IX and X, proteoglycans, gelatins, fibronectin, laminin and elastin. It plays an important role in activation of pro MMP-1 into the active form of MMP-1 in malignant tissues. MMP-3 expression is low in normal tissues but it is altered during tumour formation, where remodeling of ECM is required. Purpose of the study: To assess the association of single-nucleotide polymorphisms, Adenosine (Insertion/ Deletion) in-1171 5A > 6A in the MMP-3 promoter regions of patients with oral submucous fibrosis and in healthy individuals (controls). Methods: Thirty cases of OSF were categorized according to Khanna et al classification into four groups and Twenty age and sex matched controls were included in the study. Blood samples were collected in EDTA coated vacutainers and PCR restriction analysis was done. A statistical analysis was done using Chi-square test and Fisher's exact test to assess the frequency and association of the alleles in the case-control group. Results: The result showed a statistical significance difference between the duration of habit and disease severity with polymorphisms. The result also showed a higher frequency of the 5A allele in the study group as compared to the controls. Conclusion: A long-term follow up of these patients is mandatory to see the prognosis and their susceptibility to malignancy. The positive outcome of an association of the disease with polymorphisms would result in the development of potential diagnostic and therapeutic possibilities in potentially malignant and malignant lesions.

International Journal of Case Reports and Images, 2012

Introduction: OralFacialDigital (OFD) syndrome is the collective name of a group of rare inherite... more Introduction: OralFacialDigital (OFD) syndrome is the collective name of a group of rare inherited syndromes characterised by malformations of the mouth, face, hands and feet. Currently, nine OFD syndromes have been identified and among them OFD I and OFD II are comparatively common. These two can be distinguished from each other with the help of skeletal Xrays and by the observation of various specific symptoms and also have different patterns of inheritance. Case Report: A case report of a female child with features of OFD II also called as Mohr syndrome is presented in this study. Comparison with all the previously determined features of Mohr syndrome was done with this case and investigations were carried. Conclusion: The report showed the confirmation of Mohr Syndrome in this child along with some uncommon features like congenital heart disease.

Asian Journal of Medical Sciences, 2011

Objective: Familial Hypercholesterolemia (FH) is a metabolic disorder inherited as an autosomal d... more Objective: Familial Hypercholesterolemia (FH) is a metabolic disorder inherited as an autosomal dominant trait characterized by an increased plasma low-density lipoprotein (LDL) level. The disease is caused by several different mutations in the LDL receptor (LDLR) gene. Several mutations have been reported in this gene in patients from several ethnic groups. Early identification of individuals carrying the defective gene could be useful in reducing the risk of atherosclerosis and myocardial infraction by the available therapeutic methods. The techniques available for determining the number of the functional LDLR molecules are difficult to carry out and expensive. Our study presents mutation analysis of the LDLR gene in 24 Indian families with FH. Material & Methods: Peripheral blood samples were obtained form individuals after taking informed consent on the condition that each of these individuals had at least one first-degree relative affected with FH. Genomic DNA was isolated, exo...

Journal of Applied Biology & Biotechnology, 2018

The young shoots of Bambusa bambos are used ayurvedic medicines in India. Young shoots content va... more The young shoots of Bambusa bambos are used ayurvedic medicines in India. Young shoots content various chemical components such as cholin, betain, urease, cyanogenetic glucosides, oxalic acid, and benzoic acid. It has antiulcer, antifertility, anti-inflammatory, and antioxidant functions. In the present study, the hepatoprotective activity of methanolic shoot extract of B. bambos was tested against carbon tetrachloride (CCl 4)-induced hepatotoxicity in rats. Female Wistar rats were divided into five groups; Group I served as normal control. Groups II-VI were administered CCl4 mixed with olive oil 1:1 intraperitoneally (1 mL/kg body weight), after every 72 h for 16 days. Group II was CCl 4 negative control. Groups IV and V received methanolic shoot extract mixed with olive oil, 200 mg and 400 mg/ kg body weight, respectively. Group III received silymarin 50 mg/kg body weight for 16 days orally once daily. Methanolic extract attenuated the increase in aspartate amino transaminase (AST) and alanine amino transaminase (ALT) as well as alkaline phosphatase (ALP) and total bilirubin that occur during liver injury after CCl 4 injection. Outcome of the present study suggests that treatment with methanolic shoot extract of B. bambos-induced reduction in ALT, AST, ALP, and total bilirubin in rats indicating hepatoprotective potential of the extract.

Indian Journal of Radiology and Imaging, 2019

Objectives: To highlight the typical magnetic resonance imaging (MRI) findings in hyperglycemia-i... more Objectives: To highlight the typical magnetic resonance imaging (MRI) findings in hyperglycemia-induced seizures and compare the results with similar previous studies with a brief mention of pathophysiological mechanisms. Materials and Methods: This retrospective study included medical and imaging records of six consecutive patients with hyperglycemia-induced seizures. The data analysis included a clinical presentation and biochemical parameters at admission. The MRI sequences were evaluated for region involved, presence of subcortical T2 hypo-intensity, cortical hyper-intensity, and restricted diffusion. Similar previous studies from the National Library of Medicine (NLM) were analyzed and compared with our study. Results: Twenty-four patients were included from four studies in previous literature for comparison. In our study, on imaging, posterior cerebral region was predominantly involved, with parietal involvement in 83.3%, followed by occipital, frontal, and temporal involvemen...

The Indian journal of radiology & imaging

The purpose of our study was to assess the prevalence of variations in the extent of sphenoid pne... more The purpose of our study was to assess the prevalence of variations in the extent of sphenoid pneumatization in the Indian population and compare with existing literature. This retrospective study included 500 patients who underwent CT of the paranasal sinuses. The multiplanar reformations of paranasal sinus were assessed for the type of pneumatization of the sphenoid sinus and type of clival, lateral recess, lesser wing, and anterior recess extensions. The conchal, presellar, incomplete sellar, and complete sellar types comprised 0%, 1.2%, 22.2%, and 76.6% of patients. The extensions of pneumatization subtypes in the study population were clival in 76.6% subjects; lateral recess, lesser wing, and anterior recess in 59.7%, 20.4%, and 20.4% of sinuses, respectively. The pure forms were seen in 25.4% and combined forms in 61% of sinuses. The presellar type (1.2%) was less common and sellar type (98.8%) being common in our population compared to the Caucasian and East Asian population....

Polish Journal of Radiology, 2017

Background: Mid-esophageal region is an uncommon location of esophageal diverticula, a condition ... more Background: Mid-esophageal region is an uncommon location of esophageal diverticula, a condition usually diagnosed in elderly individuals.

Journal of neuroradiology. Journal de neuroradiologie, Jan 8, 2017

To assess the significance of association and possible correlation between hemorrhage, cytotoxic ... more To assess the significance of association and possible correlation between hemorrhage, cytotoxic edema, blood pressure and imaging severity in posterior reversible encephalopathy syndrome (PRES). This retrospective study included the medical and imaging records of 35 consecutive patients with PRES. The clinical data analysis included the highest recorded blood pressure (BP) on the day of the ictus, MRI including spin-echo echo planar diffusion-weighted imaging (DWI), susceptibility weighted angiography (SWAN) and conventional sequences. The presence of hemorrhage and cytotoxic edema was evaluated for the significance of correlation and association with each other and with blood pressure and imaging severity. On MR imaging, hemorrhage was found in 25.7%, and cytotoxic edema in 20% of patients. There was no statistically significant association of hemorrhage (P=0.403) and cytotoxic edema (P=0.162) with BP in contrast to significant association of hemorrhage (P<0.001) and cytotoxic ...

Oral Lichen planus (OLP) is a disease of adulthood, and children are rarely affected. It may invo... more Oral Lichen planus (OLP) is a disease of adulthood, and children are rarely affected. It may involve various mucosal surfaces either independently or concurrently (oral, skin, and oral and skin lesions). Prevalence of skin LP in general population is 0.9% -1.2% and prevalence of oral LP is reported between 0.1% and 2.2% (1). The relative risk for oral lichen planus was highest (13.7) among those who smoked and chewed tobacco . The etiopathogenesis appears to be complex, with interactions between genetic, environmental, lifestyle factors, and interestingly with new associations such as with liver disease have emerged. Viral infections have recently been linked with OLP. Herpes Simplex virus-1 (HSV-1), Cytomegalovirus (CMV), Human Herpes virus-6 (HHV-6), EpsteinBarr virus (EBV), Human Papilloma virus (HPV) and Hepatitis C virus (HCV) are virus types that have been studied in the etiopathogenesis of OLP(2). EBV is associated with infectious mononucleosis, oral hairy leukoplakia (nonmal...

The current investigation was focused on the assessment of antiproliferative effects of the Solan... more The current investigation was focused on the assessment of antiproliferative effects of the Solanum macranthum aqueous fruits extract against the human breast cancer cell line MDA-MB-231 in vitro. Phytochemical analysis of the S. macranthum aqueous fruits extract was performed and the phytoconstituents found positive for alkaloids, phenols, and saponins. Quantitative analysis of the total alkaloid and phenol was determined using gravimetric analysis and folins-phenols reagent method, respectively. The total alkaloid present was estimated to be 7.58% and total phenol of 158.774 mg/Gallic Acid Equivalent. The cytotoxicity of S. macranthum aqueous fruits extract was screened using 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay and camptothecin was used as standard reference drug. At the concentration of 500 μg/ml, the aqueous extract potentially inhibited the growth of the MDA-MB-231 cell line in vitro reaching 19%, and the IC50 value was calculated to be 525...

Journal of Oral and Maxillofacial Pathology : JOMFP, 2019

Introduction: The tumor-suppressor p53 protein is inactivated by the human papillomavirus (HPV) E... more Introduction: The tumor-suppressor p53 protein is inactivated by the human papillomavirus (HPV) E6 oncoprotein, causing polymorphism of the p53 at codon 72 of exon either proline (Pro) or arginine (Arg). Specific allele predisposition has been reported in the literature. The association between the p53 allele and HPV types has been reported. We analyzed the association between p53 polymorphism at codon 72 and HPV 16 and 18 genotypes in control, oral submucous fibrosis (OSF) and oral squamous cell carcinoma (OSCC). Materials and Methods: Of the total 90 cases, biopsy tissues of all groups (30 cases of OSF, OSCC and control each) were collected to extract DNA. Polymerase chain reaction was used to detect HPV 16 and 18 and alleles of codon 72 in p53 were evaluated in all the samples. Results: In control, OSF and OSCC samples showed the presence HPV 63.3%, 33.3% and 60%, respectively. In OSF, HPV 16 and 18 was detected in four and four cases, respectively, whereas in OSCC, HPV 16 and 18...

Clinical Phytoscience, 2020

Background The current study undertaken to evaluate antioxidant property of Solanum macranthum fr... more Background The current study undertaken to evaluate antioxidant property of Solanum macranthum fruit methanol extract and its in-vitro cytotoxic effects on breast cancer MDA-MB-231 and mice embryo fibroblast (MEF-L929) cell line. Methods The total alkaloids, phenols and flavonoids content in the methanol extract of S.macranthum fruit were estimated. The antioxidant activity was evaluated by FRAP assay, H2O2 scavenging assay and DPPH assay. Anti-inflammatory protein denaturation inhibition assay was carried out using bovine serum albumin. Anticancer property of the extract was evaluated against breast cancer MDA-MB-231 by MTT assay and comparative study was carried out on normal cells using MEF-L929 cell line. Results Phytochemical estimation revealed that total alkaloid content was measurable high (13.6 g/100 g of extract), where as the total phenolics and flavonoids content was 115.5 mg/GAE and 142.6 mg/QE. Further, the antioxidant effect of methanol extract by H2O2 assay showed co...

Asian Journal of Medical Sciences, 2016

Background: Oral Submucous Fibrosis is one of the oral potentially malignant disorders presenting... more Background: Oral Submucous Fibrosis is one of the oral potentially malignant disorders presenting with progressive restriction in mouth opening. The condition having a high malignant transformation rate necessitates identifi cation of biomarkers to be employed for early detection of malignant change. This will infl uence the prognosis in addition to adding better quality of life to patients. Aims and Objectives: To relate the association of VEGF-460C/T polymorphism in patients with OSMF and to compare the same among healthy subjects. Materials and Methods: Thirty patients with Oral submucous fi brosis and 20 controls free from habits and any form of lesions were included in the study. The polymorphism of VEGF gene was detected by polymerase chain reaction-based restriction analysis. Results: Sixty-four percent of the population in the study was in the age group of 21-40 yrs suggesting the prominence of disease in younger individuals with male predominance. With reference to polymorphism, 6.67% of the subjects from OSMF group showed CT polymorphism and 16.67% showed TT polymorphism. There were no statistically signifi cant differences in the polymorphism between the study group and controls. However the frequency of T allele in the patient group 12 (20%) was greater than that in the control group 1 (2.5%), which was a signifi cant fi nding. There was no association between the habits, frequency of habits, duration of quid placement, site of quid placement and style of chewing with the nature of polymorphism. Conclusion: VEGF 460C/T has the potential to be used as a prognostic marker in predicting the malignant transformation of OSMF.