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Papers by Soodabeh Rostami

African Journal of Microbiology Research, 2013

Meat products have been implicated in outbreaks of E.coli O157:H7 in most of the world. This bact... more Meat products have been implicated in outbreaks of E.coli O157:H7 in most of the world. This bacterium is associated with diseases such as hemorrhagic colitis, hemolytic uremic syndrome and thrombotic thrombocytopenic purpura. In this study, we used two Conventional methods and Multiplex PCR for the isolation and identification of E. coli O157:H7 from ground beef hamburger samples. In total, 200 fresh ground beef hamburger samples were obtained from different meat factories across Khuzestan Province from March to September 2010. Tryptone-Soya-broth (TSB) supplemented with novobiocine (20 mg/l) was used as enrichment medium and Tellurite cefixime-sorbitol MacConkey agar (TC-SMAC) was used for the detection of Non-Sorbitol Fermenting bacteria. Biochemical tests were performed on the Non-Sorbitol fermenting (NSF) colonies. Colonies confirmed as E. coli were selected as templates for Multiplex PCR method and serotyping. Out of the 200 ground beef hamburger samples, 8 samples (4%) had positive results for Non-Sorbitol fermenting colonies (NSF). Out of the 8 samples, three (38%) were confirmed as E. coli by biochemical tests. Of the 3 samples, two samples were E. coli O157:H7 based on multiplex PCR and serotyping. The results indicate that hamburgers could be a reservoir of E.coli O157:H7 in Khuzestan Province. Since this strain is a food-born pathogen, inspection of meat products for this bacterium is recommended.

bioRxiv (Cold Spring Harbor Laboratory), May 11, 2021

Bacterial cancer therapy has gained lots of attention in the past decade and is now considering a... more Bacterial cancer therapy has gained lots of attention in the past decade and is now considering a reliable option for the future. However, some concerns have limited its application into clinic settings like insufficient colonization of tumors and infectious origin of the currently used bacteria like Clostridium and Salmonella species, especially in cancer patients which exhibit different levels of immunocompromising. In the present study, Veillonella parvula (V. parvula) as a strictly anaerobic bacterium which has rarely identified as a pathogen in human, was administrated into 4T1 breast tumor-bearing mice. At first, 4T1 breast tumor-bearing BALB/c mice were injected with 10 7 bacteria intravenously, intraperitoneally, orally, or intratumorally. The best administration route according to tumor colonization and safety was selected. Then, the therapeutic effect of V. parvula administration was investigated according to the 4T1 breast tumor's growth, metastasis, and tumor-bearing mice survival. Besides, histopathological evaluations were done to estimate microscopic changes at the inner of the tumor. V. parvula exhibited significant tumor-targeting and colonization efficacy, 24 h after intravenous administration and formed clustered colonies at the central region of the tumors. Although a negligible number of the bacteria were localized at normal organs, these organs became clear from the bacteria after 72 h, and no side effects or death were observed at the animals after intravenous administration of V. parvula. Although mean tumor volumes in the V. parvula treated group was lower than the control (~ 25.4%), their difference wasn't statistically significant (P > 0.05). Despite significant tumor colonization (5500000:1 in comparison with normal organs after 72 h), V. parvula didn't cause a significant therapeutic effect on the metastasis or survival time of tumor-bearing mice. Taking together, V. parvula is a completely

Infection Control & Hospital Epidemiology, 2001

Objective: To determine the impact of methicillin-resis-tant Staphylococcus aureus (MRSA) coloniz... more Objective: To determine the impact of methicillin-resis-tant Staphylococcus aureus (MRSA) colonization on the occurrence of S aureus infections (methicillin-resistant and methicillin-suscep-tible), the use of glycopeptides, and outcome among intensive care unit (ICU) patients. Design: Prospective observational cohort survey. Setting: A medical-surgical ICU with 10 single-bed rooms in a 460-bed, tertiary-care, university-affiliated hospital. Patients: A total of 1,044 ICU patients were followed for the detection of MRSA colonization from July 1, 1995, to July, 1 1998. Methods: MRSA colonization was detected using nasal samples in all patients plus wound samples in surgical patients within 48 hours of admission or within the first 48 hours of ICU stay and weekly thereafter. MRSA infections were defined using Centers for Disease Control and Prevention standard definitions, except for ventilator-associated pneumonia and catheter-related infections, which were defined by quantitative dis...

PubMed, Feb 5, 2019

Background: In recent times, microbial-biofilm contamination has attracted considerable attention... more Background: In recent times, microbial-biofilm contamination has attracted considerable attention to the food industry. Pathogenic microorganisms can attach to food surfaces, grow on them, and form biofilm that cause an increase in the food safety risk. The mechanisms of biofilm formation have become an important issue in the food-processing industry, therefore, the aim of this study is to determine the biofilm formation and profiles of genes involved in biofilm production of staphylococci isolated from various foodstuff products. Material and methods: This cross-sectional study was conducted at some grocery stores and confectionaries from September 2015 to October 2016 in different areas of Isfahan, Iran. Staphylococcus spp were isolated from different foodstuff samples including sweet pastries, cakes and similar baked goods, dairy products such as cheese and yogurt, meat products such as sausages, and hamburgers. Standard microbiological methods were used for identification of Staphylococcus spp isolates. Antibiotic susceptibility pattern was determined by the disc diffusion method and icaA/icaD genes have been investigated as PCR target because of their role in the expression of intercellular adhesions involved in biofilm formation by S. aureus. Results: From a total of 194 different foodstuffs samples, 84 Staphylococcus spp were isolated. Out of the 84 Staphylococcus isolates, 95.2% (80/84) were positive to the ability of biofilm formation. Overall, 35.7% (30/84) and 26.2% (22/84) of Staphylococcus spp isolates were positive for icaA and icaD genes, respectively. Conclusion: The results of the present study indicate that the remarkable rate of biofilm formation with the emergence of antibiotic resistance still remains a significant risk for the food safety, especially in foodstuff samples.

Immunopathologia Persa, Feb 9, 2023

Introduction: Managing influenza (flu) due to its rapid transmission is a considerable challenge ... more Introduction: Managing influenza (flu) due to its rapid transmission is a considerable challenge for the health system. Considering the variety of clinical symptoms in influenza and recognizing its symptoms in different conditions of patients can be effective in its management. Objectives: In the present cross-sectional study, we evaluate the relationship between clinical and para-clinical findings and the treatment measures observed at the time of hospitalization of influenza patients and their conditions at the time of discharge from the hospital. Patients and Methods: Our investigation was conducted from March 2019 to March 2021 in Alzahra hospital of Isfahan, Iran. The research population included influenza patients admitted to the infectious ward. Results: A total of 122 hospitalized influenza patients (n=122) were included in this research. The number of patients with influenza A and B was 44 and 78, respectively. There was a significant relationship between the type of influenza and the patient's condition upon discharge (P = 0.001). Influenza vaccination (P < 0.001), diabetes (P = 0.038), and cardiovascular disease (P = 0.004) were significantly associated with the patient's condition at discharge. According to our investigation, among the drugs used, oseltamivir significantly reduced mortality in patients receiving it (P < <0.001). There was a statistically significant difference between the variables of all chest radiology and the patient's condition at the time of discharge (P < 0.001). Furthermore, there was a statistically significant difference between the length of hospital stay (P = 0.001), the number of white blood cells (P = 0.001), the number of platelets (P = 0.006), and the amount of C-reactive protein (CRP) (P < 0.001) with the patient's condition upon discharge. Conclusion: Among the comorbidities studied, diabetes and cardiovascular disease were significantly associated with mortality in patients with influenza. Vaccination significantly reduces mortality from influenza in high-risk patients. The antiviral drug oseltamivir is recommended as a useful drug for patients with the influenza. However, a multi-center study with larger sample size is necessary for a more conclusive result.

Journal of Medical Microbiology, Mar 1, 2019

Purpose. Community‐associated methicillin‐resistant Staphylococcus aureus (CA‐MRSA) has become a ... more Purpose. Community‐associated methicillin‐resistant Staphylococcus aureus (CA‐MRSA) has become a considerable public health concern in both developed and developing countries due to the rapid spread of this bacterium around the world, also the epidemiology of MRSA has changed, as the isolation of MRSA strains is not limited to health‐care settings or patients with predisposing risk factors. Therefore, the objective of this study is to determine the genetic diversity and antibiotic resistance profile of CA‐MRSA nasal carriage in Iranian children. Methodology. A cross‐sectional study was conducted from April 2013 to March 2014. A total of 25 CA‐MRSA were isolated from the anterior nares of 410 preschool children with no risk factors. All MRSA isolates were characterized by detection of the Panton‐Valentine leukocidin (pvl) and &ggr;‐hemolysin genes, staphylococcal cassette chromosome mec (SCCmec) typing and multi‐locus sequence typing (MLST). Results. In 25 CA‐MRSA isolates, Pvl and &ggr;‐hemolysin genes were detected in one (4%) and 18 (72%) isolates; respectively. Overall, 92% (23/25) of isolates belonged to SCCmec type IV and 8% (2/25) of them had SCCmec type V profile. Using MLST, the 25 isolates were grouped into six clonal complexes (CC) and eight sequence types (ST) (CC5/ST6, CC22/ST22 and ST217, CC30/ST30 and ST1107, CC78/ST859, CC398/ST291 and CC97/ST405). The ST859/SCCmec IV (11/25, 44%) was the predominant clone among the isolates. ST859‐MRSA‐IV‐pvl‐negative (resistant to tetracycline) have successfully adapted to the Iranian preschool children population. Conclusion. Our results suggest that the genomic diversity was observed among the CA‐MRSA. In addition, the current study demonstrates that pvl is not a reliable marker for CA‐MRSA in our region.

Jundishapur Journal of Microbiology, Jan 17, 2015

Background: Neisseria lactamica as one of the main commensal in oropharynx during the childhood i... more Background: Neisseria lactamica as one of the main commensal in oropharynx during the childhood is related to the induction of a natural immunity against meningococcal meningitis. Also Moraxella catarrhalis in oropharynx of children is a predisposing factor for otitis media infection. Objectives: The current study aimed to investigate the frequency of the N. lactamica, other nonpathogenic Neisseria spp. and M. catarrhalis in the oropharynx of young healthy children in Ahvaz, Iran by the two phenotypic tests and Polymerase Chain Reaction (PCR). Materials and Methods: A total of 192 oropharyngeal swab samples of the young healthy children were studied during four months. Swabs were plated onto enriched selective media and non-selective media. Gram-negative and oxidase-positive diplococci were identified by several conventional biochemical tests. The PCR and sequencing were used to confirm the accuracy of laboratory diagnosis to identify N. lactamica and M. catarrhalis. Results: Among 192 young healthy children with the mean age of 5.93 ± 2.5903 years, authors identified: N. lactamica (21.9%) in the age group of one to nine years; N. mucosa (6.3%); N. sicca (7.8%); N. cinerea (1.6%); N. subflava (biovar subflava) (4.2%); N. subflava (biovar perflava) (28.1%); N. subflava (biovar flava) (7.3%) and M. catarrhalis (42.7%). Conclusions: The young healthy children screening by colonization of N. lactamica and other nonpathogenic Neisseria spp. in oropharynx was the first report in Ahvaz, Iran. The study results demonstrated the high frequency of colonization of M. catarrhalis in the studied young healthy children other than Neisseria spp.

Current Pharmaceutical Design, Aug 1, 2022

: Coronavirus disease 2019 (COVID-19) is the result of infection with severe acute respiratory sy... more : Coronavirus disease 2019 (COVID-19) is the result of infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Understanding molecular pathogenesis is an essential factor for the allocation of effective preventive measures and the development of targeted therapeutics against COVID-19. The genome of SARS-CoV-2 encodes structural and nonstructural proteins, which can be targets for compounds with potential therapeutic ability. On the other hand, the virus life cycle has stages susceptible to targeting by drug compounds. Many natural antiviral compounds have been studied and evaluated at the cellular and molecular levels with antiviral potential. Meanwhile, many studies over the past few months have shown that plant polysaccharides have a good ability to target proteins and stages of the virus life cycle. In this regard, in this review study, the virus specifications and infectious process and structural and functional components of SARSCoV- 2 will be reviewed, and then the latest studies on the effect of plant compounds with more focus on polysaccharides on viral targets and their inhibitory potential on the infectious process of COVID-19 will be discussed.

Asian Pacific Journal of Tropical Medicine, 2021

Objective: To determine the antibiotic resistance patterns of the Acinetobacter (A.) baumannii co... more Objective: To determine the antibiotic resistance patterns of the Acinetobacter (A.) baumannii complex isolates that cause the confirmed infection. Methods: The present descriptive study was performed from March 2016 to March 2018 in three referral hospitals in Isfahan, Iran. All A. baumannii complex strains isolated from different clinical samples were identified by conventional phenotypic methods and antibiotic susceptibility pattern was detected. After the clinical investigation, contaminated samples were excluded and the source (hospital/community) and site of the infection were determined. Data on antibiotic susceptibility testing were extracted from WHONET software and analysis was done with SPSS. Results: From 254 patients who had confirmed A. baumannii complex infection, 158 (62.20%) cases were male, 27 (10.63%) were less than 20 years old, 172 (67.72%) had healthcare-associated infections and 96 (37.79%) were admitted in intensive care units. The most frequent infection was bloodstream infections (111, 43.70%). Our results showed that most of the isolates were resistant to most of the antibiotics (more than 75.00%) and a lower rate of non-susceptibility was observed against minocycline (20, 44.44%) and colistin (0%). The rate of multidrug-resistant isolates was 88.97%. There was no significant difference between resistance of A. baumannii complex isolates according to age. However, the resistance to amikacin and minocycline and the rate of multidrug resistance (MDR) were significantly different between males and females. In patients with healthcare associated infection (HAI), MDR isolates were significantly different regarding admission in ICU ward. Resistance to levofloxacin and ciprofloxacin were lower in isolates from patients with bloodstream infections in comparison to other diagnoses. Conclusions: In our study, a high level of antibiotic resistance was detected in both community-acquired and healthcare-associated A. baumannii complex infections. Appropriate antibiotic prescription in a clinical setting is an essential need for the control and prevention of A. baumannii resistant infections.

PubMed, Feb 5, 2019

Introduction: We aimed to determine the distribution of Phenol-soluble modulin-mec (psm-mec) gene... more Introduction: We aimed to determine the distribution of Phenol-soluble modulin-mec (psm-mec) gene and its relationship with biofilm formation in clinical methicillin-resistant S. aureus (MRSA). Methods: In a descriptive study, a total of 94 cefoxitin-resistant S. aureus isolates were collected from patients and tested for antibiotic susceptibility testing, multiplex polymerase chain reaction (MPCR) for detection of mecA and pvl genes, PCR for detection of psm-mec gene and SCCmec typing of psm-mec and pvl-positive isolates. Furthermore, isolates were tested by microtiter plate method for biofilm formation assay. Results: Multiplex PCR for detection of mecA and pvl genes was performed for all cefoxitin-resistant isolates. The mecA gene was found in 92 (97.9%) isolates but none of the isolates carried the pvl gene. Sixty-five (69.1%) isolates harbored psm-mec genes and 95.4% of these isolates belong to SCCmec type III. Statistical analysis showed a significant difference between the presence or absence of psm-mec gene and biofilm production (P<0.001). Conclusion: In this study, more than half of the MRSA strains harbored psm-mec gene and almost one-fifth of them produced strong biofilm. Since the strains with strong biofilm formation have more antibiotic resistance and cause the long-lasting infection, for the suitable treatment of hospitalized patients with this kind of MRSA strains, we should be paid more attention to these strains.

Jundishapur Journal of Microbiology, Nov 14, 2015

Background: Antigenic similarities between Neisseria lactamica as a commensal species and N. meni... more Background: Antigenic similarities between Neisseria lactamica as a commensal species and N. meningitidis serogroup B (NmB) as an important cause of meningitis infection have been considered for the development of an effective vaccine based on their common proteins to prevent life-threatening bacterial meningitis. Objectives: The main aims of this study were to determine whole proteome profiles of N. lactamica strains and to compare them with whole proteome profile of a reference strain of NmB for identification of some of common proteins between the two species. Materials and Methods: We compared the whole proteomic profiles of N. lactamica strains and a reference strain of NmB. Lysates from bacterial strains were resolved by two-dimensional gel electrophoresis (2-DE), followed by Coomassie Brilliant blue staining. Some of the protein spots were excised from the gel and subjected to matrix-assisted laser desorption/ionization-tandem time-of-flight mass spectrometry (MALDI-TOF/TOF MS) analysis. Results: The analysis of Coomassie-stained gels using ImageMaster 2D Platinum software identified approximately 800 reproducible protein spots in the range of pI 4.5-9.5 and M r of 8-100 kDa for each 2-DE gel of the studied bacterial strains. By comparing proteome maps of 2-DE gels, more than 200 common protein spots were recognized between the two species. Forty-eight common protein spots between the studied bacterial strains were identified by MALDI-TOF/TOF-MS. The results indicated that among the protein spots identified by MOLDI-TOF/TOF mass spectrometry, the groups of proteins included cell surface, energy metabolism, amino acid transport and metabolism, coenzyme metabolism, defense, multifunctional cellular processes, DNA, RNA and protein synthesis, ribosomal structure, regulatory functions, replication, transcription, translation, unknown and hypothetical proteins with unknown function. We found that N. lactamica strains have a proteome profile somewhat similar to each other and slightly different with NmB. Conclusions: These results show the usefulness of proteome analysis in successful identification of the common proteins between N. lactamica strains and NmB. This proteomics analysis is the starting point in the path of knowledge development about whole proteome profiles of N. lactamica strains.

PubMed, Mar 1, 2014

Background: Detection of latent tuberculosis infection (LTBI) in transplant candidates is very im... more Background: Detection of latent tuberculosis infection (LTBI) in transplant candidates is very important. The tuberculin skin test (TST) and interferon-gamma release assays (IGRAs) are standard immunologic tools for LTBI detection. The aim of this study was to compare the TST results and T-SPOT(®).TB test (a type of IGRAs) in kidney transplant candidates for the screening of LTBI and follow the patients with positive test for an activation of tuberculosis (TB) after transplantation and using anti-TB prophylaxis. Materials and methods: This study was a prospective study and carried out in 44 renal transplant candidates from March 2010 to February 2011 in the teaching hospitals of Isfahan University of Medical Sciences, Iran. TST and T-SPOT(®).TB test were performed and their results evaluated. Patients with a positive skin test and/or T-SPOT(®).TB test were started on anti-TB prophylaxis and followed after transplantation for an activation of their LTBI for 1 year. Results: Overall, 8 (18.2%) patients were positive for TST and 6 (13.6%) patients for T-SPOT(®).TB test. The agreement between TST and T-SPOT(®).TB test was moderate (κ = 0.49, 95% confidence interval 0.145-0.839). The overall agreement between TST and T-SPOT(®).TB test was 86%. No relation was found between the underlying diseases and TST or T-SPOT(®).TB test positivity. Although isoniazid prophylaxis was used for patients with positive TST and/or T-SPOT(®).TB test, one patient had reactivation of TB. Conclusion: In kidney transplant candidates both TST and T-SPOT(®).TB test were comparable for the diagnosis of LTBI with reasonable agreement between the tests. However, further studies are needed to determine the ability of T-SPOT(®).TB test to detect LTBI and to evaluate the need for prophylaxis in these patients.

Background: Ocular adenoviral infections occur throughout the world in both sporadic and epidemic... more Background: Ocular adenoviral infections occur throughout the world in both sporadic and epidemic forms. In the present study we determined the frequency of adenoviral conjunctivitis in two referral university hospitals in Tehran by cell culture and PCR method. Materials and methods: Specimens were scraped from the lower palpebral conjunctiva of 115 patients with conjunctivitis who had referred to Labafinejad and Imam Hossein hospitals during a 6-month period and transferred to two different media, one for cell culture and the other for PCR. Then specimens of cell culture were inoculated to Hep-2 cells and sub cultured in micro plates. Cultures were evaluated for CPE. Viral DNA was extracted from specimens and PCR technique was applied by special primers. Results: Of 115 samples, 18(15.7%) were adenovirus positive during PCR analysis, of which 14 revealed to be cell culture positive as well. Most of the sufferers from adenoviral conjunctivitis were female (61%) and aged 41-50 years (50%). In patients with adenoviral conjunctivitis, conjunctival hyperemia, pain and eyelids edema were the most common findings. Conclusion: Results have demonstrated that adenoviruses are common causative agents for viral conjunctivitis. PCR revealed to be more sensitive and accurate than cell culture for detecting adenoviral conjunctivitis.

PubMed, Mar 1, 2011

Background: Mycobacterium tuberculosis is a major cause of mortality and morbidity worldwide. Inf... more Background: Mycobacterium tuberculosis is a major cause of mortality and morbidity worldwide. Infection with this bacterium is known to induce the development of autoantibodies of which a few are also known to be diagnostic markers for some other diseases. Antineutrophil Cytoplasmic Antibodies (ANCA's) are among those autoantibodies used in clinical setting for diagnosing systemic vasculitic syndromes. Multiple studies investigated ANCA positivity in diseases other than small vessel vasculitis. Objective: This study was performed to determine the prevalence of ANCA in pulmonary tuberculosis (TB) which may lead to the false diagnosis of Wegener's granulomatosis (WG) or vice versa. Methods: In a case-control study, 32 consecutive smear positive pulmonary TB patients and 32 normal individuals were studied. All cases and controls were screened for ANCA by indirect immunofluorescent assay (IIF), and MPO and PR3 were also tested by ELISA. Results: A prenuclear pattern (P-ANCA) was detected in 25% of the cases and 6.25% of the controls and a cytoplasmic pattern (C-ANCA) was deserved in 3.1% of both the cases and the controls by IIF assay. ANCA specificities tested by ELISA in cases revealed that 75% of the cases had anti-MPO and 12.5% had anti-PR3, while in the in controls, 3.12% had anti-MPO and none had anti-PR3. The positive ANCA significantly correlated with TB (p<0.01). Conclusion: ANCA's may be observed in both TB and systemic vasculitic syndromes such as WG. Tuberculosis and WG share some clinical features. Therefore, in countries with a high prevalence of TB, one has to distinguish between these two diseases especially when no sign of extrapulmonary involvement is observed.

PubMed, Dec 1, 2013

Background and objectives: Carbapenem resistant A. baumannii is an emerging cause of nosocomial i... more Background and objectives: Carbapenem resistant A. baumannii is an emerging cause of nosocomial infections. The aims of this study were identification of the most prevalent of carbapenem resistant genes, molecular typing and antimicrobial evaluation of A.baumannii in intensive care units. Materials and methods: Two hundred and six A. baumannii were isolated from tracheal tube discharge of hospitalized patients at different intensive care units in Ahvaz, Iran. Antimicrobial susceptibility test was done on all isolates. Multiplex and singleplex PCR were performed for detection of bla OXA-23-like, bla OXA-24-like, bla OXA-51-like, bla OXA-58-like, bla VIM, bla IMP, bla SPM and bla NDM genes. Genetic relationship of all isolates was determined by REP-PCR method. Results: Out of 206 examined isolates, 198 (96.1%) isolates were resistant to imipenem and meropenem. However 3.9% isolates were sensitive to these antibiotics. The bla OXA-23-like and bla OXA-24-like genes were detected in 85% and 8.7% of strains, respectively. No bla OXA-58- like, bla IMP, bla VIM, bla SPM and bla NDM were detected. REP-PCR results showed that isolates were belonged to five genotypes: Genotype A was the most prevalent (P- value < 0.001): it was observed in 75 of 206 strains (36.4%). Genotype B, and C were found in 28.6% and 27.7%, respectively. The rate of other genotypes was as follows: D (2.4%), E (1%). Conclusion: Based on the obtained results, the rate of carbapenem resistance was high among of A. baumannii which was isolated from intensive care units patients and oxacillinase genes were the most prevalent carbapenem resistant genes. These results revealed that three clones, A, B and C of A.baumannii are common in our hospitals.

Journal of Drug Targeting, Oct 3, 2018

Abstract In the present study, alive attenuated Salmonella typhi Ty21a was introduced as a vehicl... more Abstract In the present study, alive attenuated Salmonella typhi Ty21a was introduced as a vehicle for smart delivery of gold nanoparticles to the tumours’ hypoxic regions. At the first step, the uptakes of gold nanoparticles with seven different decorations by S. typhi Ty21a was investigated using flow cytometry and inductively coupled plasma optical emission spectroscopy. The analyses demonstrated that folic acid functionalised gold nanoparticles (FA-GNPs) are the best candidates for producing the Golden Bacteria (GB). Subsequently, the GB and FA-GNPs efficacies for tumour targeting were investigated after intravenous injection to CT-26 tumour-bearing mice. The GB exhibited more GNPs delivery to the tumour in comparison with FA-GNPs. Moreover, GB injection causes more delivery of GNPs to the tumours’ central regions in comparison with tumours’ periphery. This trend is completely in reverse for FA-GNPs injected group. The ratios of peripheral to central regions’ gold concentration of the tumours were 1.95 ± 0.13 and 0.61 ± 0.10 for FA-GNPs and GB groups, respectively. This observation demonstrates higher accumulation of gold nanoparticles in the centre of the tumour due to their active delivery by the S. typhi Ty21a to the deeps of tumours.

PubMed, Dec 1, 2014

Background and objectives: Resistance to macrolide can be mediated by erm and msrA genes in Staph... more Background and objectives: Resistance to macrolide can be mediated by erm and msrA genes in Staphylococcus aureus. There are the evidences that show erm genes may be causative agent of inducible or constitutive resistance. The aim of this study was to investigate the incidence of inducible clindamycin resistance and determine the most frequency of erm and msrA genes among S. aureus isolates. Materials and methods: In this study a total of 124 non duplicated clinical isolates of S. aureus were tested with disk diffusion method. All isolates were tested by PCR for mecA, ermA, ermB, ermC and msrA genes. Results: According to PCR results, 48.4% had mecA gene and 51.6% were mecA negative. By phenotypic D-test method, 32.3% revealed inducible resistance and recorded as D and D(+). Sensitive and constitutive phenotypes were found in 54.8% and 12.9% of isolates respectively. Inducible clindamycin resistance was more prevalent in MRSA (29%) than MSSA isolates (2.4%). Among studied erm genes, the most frequency genes were ermA and ermC with 41.1% and 17.7% respectively. Three isolates of them had D phenotype, while the PCR results of erm genes were negative. All isolates were negative for ermB or msrA genes. Conclusion: Since S. aureus isolates with inducible resistance may mutate and change to constitutive resistance, to prevent treatment failure, we suggest that inducible resistance test be performed on erythromycin resistant/clindamycin sensitive isolates.

Advanced Biomedical Research, 2023

Journal of water process engineering, Dec 1, 2019

Recalcitrant compounds and high salinity in industrial wastewaters are two major inhibitory param... more Recalcitrant compounds and high salinity in industrial wastewaters are two major inhibitory parameter against the bacterial metabolism leading to necessity for the application of halotolerant microorganisms in biological treatment technologies. Hence, this study focused on the biological treatment of saline bisphenol A (BPA)containing wastewater at different total dissolved solids (TDS) (5, 10 and 15 g/L) with BPA concentration of 50 mg/L. Three sequencing batch reactors (SBR) were operated which applied different experimental conditions during 9 months. The pure adsorption behavior of BPA onto sludge was described via Freundlich isotherm model in batch experiments. The operation of sequencing reactors with 5 days hydraulic retention time (HRT) indicated that optimal removal rates exceeded 96.3%, 88% and 57% for BPA, COD, TOC, respectively, which was attained at high salinity. The organisms responsible for BPA removal appeared to be more sensitive to different operating conditions than changes in salinity; the BPA removal efficiency decreased from 96.3% to 69.8%, when HRT decreased from 5 days to 0.25 days. Results indicated that biodegradation was the predominant process for BPA removal. The biokinetic parameters in saline substrates were in the range of Y = 0.54-0.61 (mg VSS/mg BPA), k d = 0.006-0.013 (1/d), K s = 8.94-13.6 (mg BPA/L) and μ m = 0.3-0.4 (1/d). Identification of mixed consortium at high salinity was included the species Pseudomonas aeruginosa, three different Serratia marcescens, Bordetella muralis, and Bacillus subtilis by using 16S rRNA-analysis.

African Journal of Microbiology Research, 2013

bioRxiv (Cold Spring Harbor Laboratory), May 11, 2021

Infection Control & Hospital Epidemiology, 2001

PubMed, Feb 5, 2019

Immunopathologia Persa, Feb 9, 2023

Journal of Medical Microbiology, Mar 1, 2019

Jundishapur Journal of Microbiology, Jan 17, 2015

Current Pharmaceutical Design, Aug 1, 2022

Asian Pacific Journal of Tropical Medicine, 2021

PubMed, Feb 5, 2019

Jundishapur Journal of Microbiology, Nov 14, 2015

PubMed, Mar 1, 2014

PubMed, Mar 1, 2011

PubMed, Dec 1, 2013

Journal of Drug Targeting, Oct 3, 2018

PubMed, Dec 1, 2014

Advanced Biomedical Research, 2023

Journal of water process engineering, Dec 1, 2019