TERİN ADALI - Academia.edu (original) (raw)
Papers by TERİN ADALI
Journal of Biotechnology, Nov 1, 2019
Journal of Biotechnology, Nov 1, 2019
Aspergillus nidulans, and AspsarO, Aspergillus oryzae, inhibited PL by 39% and 36%, respectively.... more Aspergillus nidulans, and AspsarO, Aspergillus oryzae, inhibited PL by 39% and 36%, respectively. Fractionation of the active extracts using chloroform followed by DiAION HP-20 column eluted with 50% and 100% methanol revealed that 100% methanol fractions of both isolates were the most active ones with IC50 of 5.85 g/ml and 7.48 g/ml, respectively compared to orlistat (3.72 g/ml). Isolating and purification of the bioactive compounds is underway by column chromatography.
The Natural products journal, Mar 1, 2013
Diagnostic and interventional radiology, May 13, 2023
PURPOSE Osteoporosis is the systematic degeneration of the human skeleton, with consequences rang... more PURPOSE Osteoporosis is the systematic degeneration of the human skeleton, with consequences ranging from a reduced quality of life to mortality. Therefore, the prediction of osteoporosis reduces risks and supports patients in taking precautions. Deep-learning and specific models achieve highly accurate results using different imaging modalities. The primary purpose of this research was to develop unimodal and multimodal deep-learning-based diagnostic models to predict bone mineral loss of the lumbar vertebrae using magnetic resonance (MR) and computed tomography (CT) imaging. METHODS Patients who received both lumbar dual-energy X-ray absorptiometry (DEXA) and MRI (n = 120) or CT (n = 100) examinations were included in this study. Unimodal and multimodal convolutional neural networks (CNNs) with dual blocks were proposed to predict osteoporosis using lumbar vertebrae MR and CT examinations in separate and combined datasets. Bone mineral density values obtained by DEXA were used as reference data. The proposed models were compared with a CNN model and six benchmark pre-trained deep-learning models. RESULTS The proposed unimodal model obtained 96.54%, 98.84%, and 96.76% balanced accuracy for MRI, CT, and combined datasets, respectively, while the multimodal model achieved 98.90% balanced accuracy in 5-fold cross-validation experiments. Furthermore, the models obtained 95.68%-97.91% accuracy with a hold-out validation dataset. In addition, comparative experiments demonstrated that the proposed models yielded superior results by providing more effective feature extraction in dual blocks to predict osteoporosis. CONCLUSION This study demonstrated that osteoporosis was accurately predicted by the proposed models using both MR and CT images, and a multimodal approach improved the prediction of osteoporosis. With further research involving prospective studies with a larger number of patients, there may be an opportunity to implement these technologies into clinical practice.
Bio-medical Materials and Engineering, Jan 11, 2023
BACKGROUND: Hydrogel is a three-dimensional structure that has the potential to absorb and retain... more BACKGROUND: Hydrogel is a three-dimensional structure that has the potential to absorb and retain water within the mesh of its porous network structure. Currently hydrogels made from natural biopolymers are preferred in the discipline of biomedical applications because of their blood compatibility, adhesion of platelets and protein binding, ease of administration and delivery of ingredients to the place of action. OBJECTIVE: The aim of this work was to prepare a hydrogel from natural biopolymers and evaluate its blood compatibility, swelling nature, prolonged degradation and morphological features in order to further recommend its clinical use. METHODS: To prepare hydrogels, different combinations of gelatin, dialyzed SF, curcumin and N, N methylene bisacrylamide (MBA) were evenly mixed on a magnetic stirrer. After an hour of the gelation process it was kept in a refrigerator at 4 °C. For the characterization and biocompatibility studies of hydrogel, the swelling test and biodegradation analysis, SEM, FTIR, in vitro coagulation tests, total serum albumin and cholesterol level analysis were applied. RESULTS: Injectable hydrogels were successfully made with significantly correlated combinations of polymers. The analysis of physiochemical biocompatibility studies and morphological characterization were done effectively. CONCLUSION: The results of the study indicate that hydrogels made from natural biopolymers are a potential source and suitable matrices with excellent biocompatible nature acting as a useful device in delivering drugs.
Research Square (Research Square), Mar 22, 2022
Background Hydrogel is a structure with three-dimension that have the potential to absorb and ret... more Background Hydrogel is a structure with three-dimension that have the potential to absorb and retain water inside of it. Currently hydrogels made from natural biopolymers are preferred in the discipline of biomedicine due to their nature of blood compatibility, adhesion of platelets and protein binding, ease of administration and delivery of ingredients to the place of action. The purpose of this study is preparing blood compatible injectable hydrogels from naturally obtained polymers of biomaterials in a combination of different proportion, characterizing and analyzing its physiochemical blood compatibility and morphological structures. Methods Three different compositions of gelatin, dialyzed SF, curcumin and N, N methylene bisacrylamide (MBA) in different groups coded by IVA, IVB and IVC were used. The combinations were evenly mixed on a magnetic stirrer. After an hour of the gelation process it has been kept under refrigerator at 4°C. For characterization and biocompatibility studies of hydrogel swelling test and biodegradation analysis, SEM, FTIR, In-vitro coagulation tests, total serum albumin and cholesterol level analysis were applied, Results Injectable hydrogel successfully made from the combinations of SF/GE/CU/ in the presence of a cross linker MBA and the result from physiochemical biocompatibility and morphological characteristics study were con rmed that natural biopolymers used in this study are a candidate for biomedical applications. Conclusion The result con rmed that the composition coded by IVC was identi ed the most stable composition and suitable in its morphological structure with excellent blood compatible nature recommended for further biomedical applications.
Journal of Biotechnology, 2019
Aspergillus nidulans, and AspsarO, Aspergillus oryzae, inhibited PL by 39% and 36%, respectively.... more Aspergillus nidulans, and AspsarO, Aspergillus oryzae, inhibited PL by 39% and 36%, respectively. Fractionation of the active extracts using chloroform followed by DiAION HP-20 column eluted with 50% and 100% methanol revealed that 100% methanol fractions of both isolates were the most active ones with IC50 of 5.85 g/ml and 7.48 g/ml, respectively compared to orlistat (3.72 g/ml). Isolating and purification of the bioactive compounds is underway by column chromatography.
IEEE Transactions on Biomedical Engineering, 2010
Abstract This index covers all technical items-papers, correspondence, reviews, etc.-that appeare... more Abstract This index covers all technical items-papers, correspondence, reviews, etc.-that appeared in this periodical during the year, and items from previous years that were commented upon or corrected in this year. Departments and other items may also be covered if they have been judged to have archival value. The Author Index contains the primary entry for each item, listed under the first author's name. The primary entry includes the coauthors' names, the title of the paper or other item, and its location, specified by the ...
Journal of Biotechnology, 2017
Polymers
Glaucoma is extensively treated with topical eye drops containing drugs. However, the retention t... more Glaucoma is extensively treated with topical eye drops containing drugs. However, the retention time of the loaded drugs and the in vivo bioavailability of the drugs are highly influenced before reaching the targeted area sufficiently, due to physiological and anatomical barriers of the eye, such as rapid nasolacrimal drainage. Poor intraocular penetration and frequent administration may also cause ocular cytotoxicity. A novel approach to overcome these drawbacks is the use of injectable hydrogels administered intravitreously for sustained drug delivery to the target site. These injectable hydrogels are used as nanocarriers to intimately interact with specific diseased ocular tissues to increase the therapeutic efficacy and drug bioavailability of the anti-glaucomic drugs. The human eye is very delicate, and is sensitive to contact with any foreign body material. However, natural biopolymers are non-reactive, biocompatible, biodegradable, and lack immunogenic and inflammatory respon...
Cyprus Journal of Medical Sciences, 2021
BACKGROUND/AIMS Silkworms and spiders produce silk fibroin (SF). SF protein has unique characteri... more BACKGROUND/AIMS Silkworms and spiders produce silk fibroin (SF). SF protein has unique characteristics, which includes mechanical properties, biodegradation, biocompatibility, and the ability to support the differentiation of stem cells along the osteogenic lineage. These characteristics makes SF a favorable biomaterial for cartilage tissue engineering. The aim of this study was to design a SF biofilm and then to test the biocompatibility and cytotoxicity of the designed SF biofilm. MATERIAL and METHODS Characterization was executed by scanning electron microscopy and Fourier transform infrared spectrophotometer analysis spectrophotometry. Normal human articular chondrocytes were seeded on biofilm and cultured up to 2 weeks (5% CO 2 , 95% air and 37 C) under the standard culture conditions. Phase contrast microscopy and cell proliferation assay (3-(4, 5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay) was applied for evaluation of cell attachment and cell growth. RESULTS The viability of cells was linearly correlated with optical density, and chondrocyte viability in the SF film was found to be significantly higher. CONCLUSION These results indicated that SF film supports cell proliferation without side effects and the SF film is a potential material as a cartilage tissue engineering matrix.
Bu calismanin amaci; Ortaogretim kurumlarini yaratici orgut kulturu acisindan degerlendirmek, bu ... more Bu calismanin amaci; Ortaogretim kurumlarini yaratici orgut kulturu acisindan degerlendirmek, bu orgutlerin kulturunu olusturan yoneticilerin yeterlilik ve yeteneksizliklerini belirlemek, yonetici ve ogretmenlerin goruslerinden yararlanarak cozum onerileri gelistirmektir. Arastirmanin evreni, 2009-2010 ogrenim yilinda, Milli Egitim Genclik ve Spor Bakanligi Lefkosa sinirlari icerisinde bulunan 4 ortaogretim okulunda gorev yapan yonetici (mudur ve mudur yardimcilari) ve ogretmenlerden olusmaktadir. Arastirmanin orneklemi ise 26 yonetici ve 191 ogretmen olmak uzere toplam 217 katilimcidir. Veri toplama araci olarak kullanilan anket iki bolumden olusmaktadir. Birinci bolumde ‘cinsiyet, kidem, ogrenim durumu, gorev’ degiskenlerini iceren kisisel bilgi formu, ikinci bolumde ise, 24 maddeden olusan ‘Yaratici Orgut Kulturunu Olusturan Yonetici Tutumlari’ olcegi bulunmaktadir. Anket, orneklemdeki 30 ortaogretim okulu yoneticisine ve 270 ogretmene uygulanmistir. Elde edilen veriler ...
Molecules, 2020
Breast cancer ranks as the fifth leading cause of death worldwide. Chemotherapy is commonly used ... more Breast cancer ranks as the fifth leading cause of death worldwide. Chemotherapy is commonly used directly or as neo-adjuvant therapy for the management of breast cancer with its attendant adverse effects, underscoring the need to develop biocompatible bioactive compounds for pharmacological applications. The aim of this study is to encapsulate carboplatin (CP) with silk fibroin protein (SF) by using an ionic gelation method as a drug carrier system and assess the apoptotic effect on MCF-7 breast cancer cells during in vitro studies. The characterization of silk fibroin encapsulated carboplatin (SFCP) microparticles was analyzed by FTIR spectrophotometer, SEM, Mastersizer, and biodegradation methods. The encapsulation efficiency and release profile of SFCP microparticles were analyzed by an indirect UV–Vis spectrophotometric method. An apoptotic screening of MCF-7 cells was carried out with 10–200 µg/mL CP loaded SFCP, which were cultured for 24, 48, and 72 h. Data were analyzed usin...
Current Drug Targets, 2020
Alzheimer’s disease (AD) is the most common neurodegenerative disease, and is part of a massive a... more Alzheimer’s disease (AD) is the most common neurodegenerative disease, and is part of a massive and growing health care burden that is destroying the cognitive function of more than 50 million individuals worldwide. Today, therapeutic options are limited to approaches with mild symptomatic benefits. The failure in developing effective drugs is attributed to, but not limited to the highly heterogeneous nature of AD with multiple underlying hypotheses and multifactorial pathology. In addition, targeted drug delivery to the central nervous system (CNS), for the diagnosis and therapy of neurological diseases like AD, is restricted by the challenges posed by blood-brain interfaces surrounding the CNS, limiting the bioavailability of therapeutics. Research done over the last decade has focused on developing new strategies to overcome these limitations and successfully deliver drugs to the CNS. Nanoparticles, that are capable of encapsulating drugs with sustained drug release profiles and ...
Journal of Biotechnology, 2019
Long cycle performance at a high charge-discharge current of 2 A/g for the amorphous MnO 2 (a-MnO... more Long cycle performance at a high charge-discharge current of 2 A/g for the amorphous MnO 2 (a-MnO 2) and single walled carbon nanotubes (SWNTs) composites have been studied for the first time. The a-MnO 2 and SWNT composites have been successfully synthesized via a novel room temperature route starting with KMnO 4 , ethanol and commercial SWNTs. Homogeneity of the synthesized composites was established by electron microscopic studies. Electrochemical properties of the synthesized composites were elucidated by cyclic voltammetry, galvanostatic charge-discharge and electrochemical impedance spectroscopy. All the composites with different SWNT loads showed excellent cycling capability, even at the high current of 2 A/g, with the MnO 2 :20 wt% SWNT composite showing the best combination of coloumbic efficiency of 75% and specific capacitance of 110 F/g after 750 cycles. However, the composite with 5 wt% SWNTs showed the highest specific capacitance during initial cycles.
International Journal of Biological Macromolecules, 2018
Poly(N-hydroxy ethyl acrylamide) was grafted onto chitosan in aqueous acidic medium using potassi... more Poly(N-hydroxy ethyl acrylamide) was grafted onto chitosan in aqueous acidic medium using potassium per sulphate initiator to obtain polymer surfaces with blood compatibility for potential biomedical applications. Thermally cross-linked products were obtained upon drying at 60°C overnight. Chemically cross-linked films were prepared using methylene bis acrylamide (MBA) cross-linker. The products were characterized by FTIR, XRD and SEM analyses. Protein adsorption onto the film surfaces gave, on the average, 30% bovine serum albumin (BSA) and 10% human serum albumin (HSA) removal from aqueous solution, in vitro. Blood compatibility was evaluated with respect to activated prothrombin time (PT), activated partial thromboplastin time (APTT), and platelet adhesion. PT and APTT values remained within normal ranges after blood-polymer contact, with chitosan-graft-polyHEAA films, in vitro. Chitosan-graft-MBA or chitosan-graft-(polyHEAA;MBA), and blank chitosan films produced higher PT and APTT values under similar experimental conditions with chitosan-graft-polyHEAA films, exhibiting blood anticoagulant activity. SEM pictures taken before and after contact with blood sample did not reveal any significant blood component adhesion on the chitosan-graft-(polyHEAA;MBA) film surface contrary to the observation made on the blank chitosan film.
International Journal of Biological Macromolecules, 2018
The chondrocyte cell proliferation of a chitosan/silk fibroin/egg shell membrane hydrogels. Bioma... more The chondrocyte cell proliferation of a chitosan/silk fibroin/egg shell membrane hydrogels. Biomac (2018),
Critical reviews in eukaryotic gene expression, 2018
Breast cancer has continued to be a cause of increasing morbidity and mortality in women, being t... more Breast cancer has continued to be a cause of increasing morbidity and mortality in women, being the most common cause of cancer-related deaths among them. Its management using chemotherapy is continually plagued with problems of systemic toxicity, lack of compliance by patients, and inadequate targeting of cancer cells. The future of breast cancer chemotherapy will likely involve the use of biocompatible high cell-targeting capacity drug-delivery vehicles like silk fibroin to ameliorate these problems. The utilization of silk fibroin nanoparticles to deliver cytotoxic drugs provides specificity, optimal entrapment, improved therapeutic index, and maximal breast cancer cell toxicity with minimal or no collateral damage to surrounding normal cells. The silk fibroin obtained from the cocoon of the Bombyx morii worm is processed and degummed to remove the sericin component; it is then made into nanoparticles utilizing the desolvation, ionic gelation, or electrospray method. It is then l...
Critical reviews in eukaryotic gene expression, 2018
Scaffolds are important tools in tissue engineering and play a unique role in tissue regeneration... more Scaffolds are important tools in tissue engineering and play a unique role in tissue regeneration and repair of damaged tissues. A variety of natural, synthetic, and composite scaffold types can be used in cartilage tissue engineering. The limited capability of cartilage to repair itself has always been a problem during recovery from damage. The success of cartilage regeneration is dependent on a couple of factors, but the basic properties of scaffolds are biocompatibility, degradability under physiological conditions, and structural support for cell attachment. In this review, we summarize the use of different scaffold types in cartilage regeneration.
Journal of Applied Pharmaceutical Science, 2014
Understanding the mode involved in the binding of certain molecules to DNA is of prime importance... more Understanding the mode involved in the binding of certain molecules to DNA is of prime importance, and PEG offers wide-ranging applications in biological, medical and pharmaceutical contexts. FTIR spectroscopy has been used to characterize how the formed biocomplexes bind or dissociate to/from each other between PEG400-ctDNA under different conditions. Characterization and investigation of the effect of incubation time on PEG400-ctDNA biocomplexes formation were studied through spectroscopic technique FTIR. The influence of time duration and incubation on intermolecular interaction was analysed at three different selected times (Zero, 1hr, and 48 hrs.) at 1:1 PEG400-ctDNA monomer to nucleotide ratio. The experiment was carried out at room temperature 22 ºC, with prior vortex stirrer of biocomplex for 10 min to improve homogeneity of sample. The results showed that the binding reaction of PEG400-ctDNA proceeds rapidly through DNA base pairs and phosphate DNA backbone, and complexation was reached after a maximum 1hr after mixing PEG400 and ctDNA at 1:1 ratio. FTIR spectroscopy results suggest that PEG400 binds with ctDNA by weak to moderate biocomplexes formation, with both hydrophilic and hydrophobic contact through DNA base pairs, with minor binding preference towards phosphate backbone of DNA helix. The mode of interaction most likely referred to an interaction through outside groove binding or electrostatic binding modes. FTIR highlighted the significant effect of incubation time on the stable biocomplexes of non-ionic PEG400 and ctDNA. Moreover, FTIR spectroscopy technique was rapid, showed good stability, and is a valuable tool for studying the biological properties of biocomplexes of PEG400 and ctDNA.
Journal of Biotechnology, Nov 1, 2019
Journal of Biotechnology, Nov 1, 2019
Aspergillus nidulans, and AspsarO, Aspergillus oryzae, inhibited PL by 39% and 36%, respectively.... more Aspergillus nidulans, and AspsarO, Aspergillus oryzae, inhibited PL by 39% and 36%, respectively. Fractionation of the active extracts using chloroform followed by DiAION HP-20 column eluted with 50% and 100% methanol revealed that 100% methanol fractions of both isolates were the most active ones with IC50 of 5.85 g/ml and 7.48 g/ml, respectively compared to orlistat (3.72 g/ml). Isolating and purification of the bioactive compounds is underway by column chromatography.
The Natural products journal, Mar 1, 2013
Diagnostic and interventional radiology, May 13, 2023
PURPOSE Osteoporosis is the systematic degeneration of the human skeleton, with consequences rang... more PURPOSE Osteoporosis is the systematic degeneration of the human skeleton, with consequences ranging from a reduced quality of life to mortality. Therefore, the prediction of osteoporosis reduces risks and supports patients in taking precautions. Deep-learning and specific models achieve highly accurate results using different imaging modalities. The primary purpose of this research was to develop unimodal and multimodal deep-learning-based diagnostic models to predict bone mineral loss of the lumbar vertebrae using magnetic resonance (MR) and computed tomography (CT) imaging. METHODS Patients who received both lumbar dual-energy X-ray absorptiometry (DEXA) and MRI (n = 120) or CT (n = 100) examinations were included in this study. Unimodal and multimodal convolutional neural networks (CNNs) with dual blocks were proposed to predict osteoporosis using lumbar vertebrae MR and CT examinations in separate and combined datasets. Bone mineral density values obtained by DEXA were used as reference data. The proposed models were compared with a CNN model and six benchmark pre-trained deep-learning models. RESULTS The proposed unimodal model obtained 96.54%, 98.84%, and 96.76% balanced accuracy for MRI, CT, and combined datasets, respectively, while the multimodal model achieved 98.90% balanced accuracy in 5-fold cross-validation experiments. Furthermore, the models obtained 95.68%-97.91% accuracy with a hold-out validation dataset. In addition, comparative experiments demonstrated that the proposed models yielded superior results by providing more effective feature extraction in dual blocks to predict osteoporosis. CONCLUSION This study demonstrated that osteoporosis was accurately predicted by the proposed models using both MR and CT images, and a multimodal approach improved the prediction of osteoporosis. With further research involving prospective studies with a larger number of patients, there may be an opportunity to implement these technologies into clinical practice.
Bio-medical Materials and Engineering, Jan 11, 2023
BACKGROUND: Hydrogel is a three-dimensional structure that has the potential to absorb and retain... more BACKGROUND: Hydrogel is a three-dimensional structure that has the potential to absorb and retain water within the mesh of its porous network structure. Currently hydrogels made from natural biopolymers are preferred in the discipline of biomedical applications because of their blood compatibility, adhesion of platelets and protein binding, ease of administration and delivery of ingredients to the place of action. OBJECTIVE: The aim of this work was to prepare a hydrogel from natural biopolymers and evaluate its blood compatibility, swelling nature, prolonged degradation and morphological features in order to further recommend its clinical use. METHODS: To prepare hydrogels, different combinations of gelatin, dialyzed SF, curcumin and N, N methylene bisacrylamide (MBA) were evenly mixed on a magnetic stirrer. After an hour of the gelation process it was kept in a refrigerator at 4 °C. For the characterization and biocompatibility studies of hydrogel, the swelling test and biodegradation analysis, SEM, FTIR, in vitro coagulation tests, total serum albumin and cholesterol level analysis were applied. RESULTS: Injectable hydrogels were successfully made with significantly correlated combinations of polymers. The analysis of physiochemical biocompatibility studies and morphological characterization were done effectively. CONCLUSION: The results of the study indicate that hydrogels made from natural biopolymers are a potential source and suitable matrices with excellent biocompatible nature acting as a useful device in delivering drugs.
Research Square (Research Square), Mar 22, 2022
Background Hydrogel is a structure with three-dimension that have the potential to absorb and ret... more Background Hydrogel is a structure with three-dimension that have the potential to absorb and retain water inside of it. Currently hydrogels made from natural biopolymers are preferred in the discipline of biomedicine due to their nature of blood compatibility, adhesion of platelets and protein binding, ease of administration and delivery of ingredients to the place of action. The purpose of this study is preparing blood compatible injectable hydrogels from naturally obtained polymers of biomaterials in a combination of different proportion, characterizing and analyzing its physiochemical blood compatibility and morphological structures. Methods Three different compositions of gelatin, dialyzed SF, curcumin and N, N methylene bisacrylamide (MBA) in different groups coded by IVA, IVB and IVC were used. The combinations were evenly mixed on a magnetic stirrer. After an hour of the gelation process it has been kept under refrigerator at 4°C. For characterization and biocompatibility studies of hydrogel swelling test and biodegradation analysis, SEM, FTIR, In-vitro coagulation tests, total serum albumin and cholesterol level analysis were applied, Results Injectable hydrogel successfully made from the combinations of SF/GE/CU/ in the presence of a cross linker MBA and the result from physiochemical biocompatibility and morphological characteristics study were con rmed that natural biopolymers used in this study are a candidate for biomedical applications. Conclusion The result con rmed that the composition coded by IVC was identi ed the most stable composition and suitable in its morphological structure with excellent blood compatible nature recommended for further biomedical applications.
Journal of Biotechnology, 2019
Aspergillus nidulans, and AspsarO, Aspergillus oryzae, inhibited PL by 39% and 36%, respectively.... more Aspergillus nidulans, and AspsarO, Aspergillus oryzae, inhibited PL by 39% and 36%, respectively. Fractionation of the active extracts using chloroform followed by DiAION HP-20 column eluted with 50% and 100% methanol revealed that 100% methanol fractions of both isolates were the most active ones with IC50 of 5.85 g/ml and 7.48 g/ml, respectively compared to orlistat (3.72 g/ml). Isolating and purification of the bioactive compounds is underway by column chromatography.
IEEE Transactions on Biomedical Engineering, 2010
Abstract This index covers all technical items-papers, correspondence, reviews, etc.-that appeare... more Abstract This index covers all technical items-papers, correspondence, reviews, etc.-that appeared in this periodical during the year, and items from previous years that were commented upon or corrected in this year. Departments and other items may also be covered if they have been judged to have archival value. The Author Index contains the primary entry for each item, listed under the first author's name. The primary entry includes the coauthors' names, the title of the paper or other item, and its location, specified by the ...
Journal of Biotechnology, 2017
Polymers
Glaucoma is extensively treated with topical eye drops containing drugs. However, the retention t... more Glaucoma is extensively treated with topical eye drops containing drugs. However, the retention time of the loaded drugs and the in vivo bioavailability of the drugs are highly influenced before reaching the targeted area sufficiently, due to physiological and anatomical barriers of the eye, such as rapid nasolacrimal drainage. Poor intraocular penetration and frequent administration may also cause ocular cytotoxicity. A novel approach to overcome these drawbacks is the use of injectable hydrogels administered intravitreously for sustained drug delivery to the target site. These injectable hydrogels are used as nanocarriers to intimately interact with specific diseased ocular tissues to increase the therapeutic efficacy and drug bioavailability of the anti-glaucomic drugs. The human eye is very delicate, and is sensitive to contact with any foreign body material. However, natural biopolymers are non-reactive, biocompatible, biodegradable, and lack immunogenic and inflammatory respon...
Cyprus Journal of Medical Sciences, 2021
BACKGROUND/AIMS Silkworms and spiders produce silk fibroin (SF). SF protein has unique characteri... more BACKGROUND/AIMS Silkworms and spiders produce silk fibroin (SF). SF protein has unique characteristics, which includes mechanical properties, biodegradation, biocompatibility, and the ability to support the differentiation of stem cells along the osteogenic lineage. These characteristics makes SF a favorable biomaterial for cartilage tissue engineering. The aim of this study was to design a SF biofilm and then to test the biocompatibility and cytotoxicity of the designed SF biofilm. MATERIAL and METHODS Characterization was executed by scanning electron microscopy and Fourier transform infrared spectrophotometer analysis spectrophotometry. Normal human articular chondrocytes were seeded on biofilm and cultured up to 2 weeks (5% CO 2 , 95% air and 37 C) under the standard culture conditions. Phase contrast microscopy and cell proliferation assay (3-(4, 5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay) was applied for evaluation of cell attachment and cell growth. RESULTS The viability of cells was linearly correlated with optical density, and chondrocyte viability in the SF film was found to be significantly higher. CONCLUSION These results indicated that SF film supports cell proliferation without side effects and the SF film is a potential material as a cartilage tissue engineering matrix.
Bu calismanin amaci; Ortaogretim kurumlarini yaratici orgut kulturu acisindan degerlendirmek, bu ... more Bu calismanin amaci; Ortaogretim kurumlarini yaratici orgut kulturu acisindan degerlendirmek, bu orgutlerin kulturunu olusturan yoneticilerin yeterlilik ve yeteneksizliklerini belirlemek, yonetici ve ogretmenlerin goruslerinden yararlanarak cozum onerileri gelistirmektir. Arastirmanin evreni, 2009-2010 ogrenim yilinda, Milli Egitim Genclik ve Spor Bakanligi Lefkosa sinirlari icerisinde bulunan 4 ortaogretim okulunda gorev yapan yonetici (mudur ve mudur yardimcilari) ve ogretmenlerden olusmaktadir. Arastirmanin orneklemi ise 26 yonetici ve 191 ogretmen olmak uzere toplam 217 katilimcidir. Veri toplama araci olarak kullanilan anket iki bolumden olusmaktadir. Birinci bolumde ‘cinsiyet, kidem, ogrenim durumu, gorev’ degiskenlerini iceren kisisel bilgi formu, ikinci bolumde ise, 24 maddeden olusan ‘Yaratici Orgut Kulturunu Olusturan Yonetici Tutumlari’ olcegi bulunmaktadir. Anket, orneklemdeki 30 ortaogretim okulu yoneticisine ve 270 ogretmene uygulanmistir. Elde edilen veriler ...
Molecules, 2020
Breast cancer ranks as the fifth leading cause of death worldwide. Chemotherapy is commonly used ... more Breast cancer ranks as the fifth leading cause of death worldwide. Chemotherapy is commonly used directly or as neo-adjuvant therapy for the management of breast cancer with its attendant adverse effects, underscoring the need to develop biocompatible bioactive compounds for pharmacological applications. The aim of this study is to encapsulate carboplatin (CP) with silk fibroin protein (SF) by using an ionic gelation method as a drug carrier system and assess the apoptotic effect on MCF-7 breast cancer cells during in vitro studies. The characterization of silk fibroin encapsulated carboplatin (SFCP) microparticles was analyzed by FTIR spectrophotometer, SEM, Mastersizer, and biodegradation methods. The encapsulation efficiency and release profile of SFCP microparticles were analyzed by an indirect UV–Vis spectrophotometric method. An apoptotic screening of MCF-7 cells was carried out with 10–200 µg/mL CP loaded SFCP, which were cultured for 24, 48, and 72 h. Data were analyzed usin...
Current Drug Targets, 2020
Alzheimer’s disease (AD) is the most common neurodegenerative disease, and is part of a massive a... more Alzheimer’s disease (AD) is the most common neurodegenerative disease, and is part of a massive and growing health care burden that is destroying the cognitive function of more than 50 million individuals worldwide. Today, therapeutic options are limited to approaches with mild symptomatic benefits. The failure in developing effective drugs is attributed to, but not limited to the highly heterogeneous nature of AD with multiple underlying hypotheses and multifactorial pathology. In addition, targeted drug delivery to the central nervous system (CNS), for the diagnosis and therapy of neurological diseases like AD, is restricted by the challenges posed by blood-brain interfaces surrounding the CNS, limiting the bioavailability of therapeutics. Research done over the last decade has focused on developing new strategies to overcome these limitations and successfully deliver drugs to the CNS. Nanoparticles, that are capable of encapsulating drugs with sustained drug release profiles and ...
Journal of Biotechnology, 2019
Long cycle performance at a high charge-discharge current of 2 A/g for the amorphous MnO 2 (a-MnO... more Long cycle performance at a high charge-discharge current of 2 A/g for the amorphous MnO 2 (a-MnO 2) and single walled carbon nanotubes (SWNTs) composites have been studied for the first time. The a-MnO 2 and SWNT composites have been successfully synthesized via a novel room temperature route starting with KMnO 4 , ethanol and commercial SWNTs. Homogeneity of the synthesized composites was established by electron microscopic studies. Electrochemical properties of the synthesized composites were elucidated by cyclic voltammetry, galvanostatic charge-discharge and electrochemical impedance spectroscopy. All the composites with different SWNT loads showed excellent cycling capability, even at the high current of 2 A/g, with the MnO 2 :20 wt% SWNT composite showing the best combination of coloumbic efficiency of 75% and specific capacitance of 110 F/g after 750 cycles. However, the composite with 5 wt% SWNTs showed the highest specific capacitance during initial cycles.
International Journal of Biological Macromolecules, 2018
Poly(N-hydroxy ethyl acrylamide) was grafted onto chitosan in aqueous acidic medium using potassi... more Poly(N-hydroxy ethyl acrylamide) was grafted onto chitosan in aqueous acidic medium using potassium per sulphate initiator to obtain polymer surfaces with blood compatibility for potential biomedical applications. Thermally cross-linked products were obtained upon drying at 60°C overnight. Chemically cross-linked films were prepared using methylene bis acrylamide (MBA) cross-linker. The products were characterized by FTIR, XRD and SEM analyses. Protein adsorption onto the film surfaces gave, on the average, 30% bovine serum albumin (BSA) and 10% human serum albumin (HSA) removal from aqueous solution, in vitro. Blood compatibility was evaluated with respect to activated prothrombin time (PT), activated partial thromboplastin time (APTT), and platelet adhesion. PT and APTT values remained within normal ranges after blood-polymer contact, with chitosan-graft-polyHEAA films, in vitro. Chitosan-graft-MBA or chitosan-graft-(polyHEAA;MBA), and blank chitosan films produced higher PT and APTT values under similar experimental conditions with chitosan-graft-polyHEAA films, exhibiting blood anticoagulant activity. SEM pictures taken before and after contact with blood sample did not reveal any significant blood component adhesion on the chitosan-graft-(polyHEAA;MBA) film surface contrary to the observation made on the blank chitosan film.
International Journal of Biological Macromolecules, 2018
The chondrocyte cell proliferation of a chitosan/silk fibroin/egg shell membrane hydrogels. Bioma... more The chondrocyte cell proliferation of a chitosan/silk fibroin/egg shell membrane hydrogels. Biomac (2018),
Critical reviews in eukaryotic gene expression, 2018
Breast cancer has continued to be a cause of increasing morbidity and mortality in women, being t... more Breast cancer has continued to be a cause of increasing morbidity and mortality in women, being the most common cause of cancer-related deaths among them. Its management using chemotherapy is continually plagued with problems of systemic toxicity, lack of compliance by patients, and inadequate targeting of cancer cells. The future of breast cancer chemotherapy will likely involve the use of biocompatible high cell-targeting capacity drug-delivery vehicles like silk fibroin to ameliorate these problems. The utilization of silk fibroin nanoparticles to deliver cytotoxic drugs provides specificity, optimal entrapment, improved therapeutic index, and maximal breast cancer cell toxicity with minimal or no collateral damage to surrounding normal cells. The silk fibroin obtained from the cocoon of the Bombyx morii worm is processed and degummed to remove the sericin component; it is then made into nanoparticles utilizing the desolvation, ionic gelation, or electrospray method. It is then l...
Critical reviews in eukaryotic gene expression, 2018
Scaffolds are important tools in tissue engineering and play a unique role in tissue regeneration... more Scaffolds are important tools in tissue engineering and play a unique role in tissue regeneration and repair of damaged tissues. A variety of natural, synthetic, and composite scaffold types can be used in cartilage tissue engineering. The limited capability of cartilage to repair itself has always been a problem during recovery from damage. The success of cartilage regeneration is dependent on a couple of factors, but the basic properties of scaffolds are biocompatibility, degradability under physiological conditions, and structural support for cell attachment. In this review, we summarize the use of different scaffold types in cartilage regeneration.
Journal of Applied Pharmaceutical Science, 2014
Understanding the mode involved in the binding of certain molecules to DNA is of prime importance... more Understanding the mode involved in the binding of certain molecules to DNA is of prime importance, and PEG offers wide-ranging applications in biological, medical and pharmaceutical contexts. FTIR spectroscopy has been used to characterize how the formed biocomplexes bind or dissociate to/from each other between PEG400-ctDNA under different conditions. Characterization and investigation of the effect of incubation time on PEG400-ctDNA biocomplexes formation were studied through spectroscopic technique FTIR. The influence of time duration and incubation on intermolecular interaction was analysed at three different selected times (Zero, 1hr, and 48 hrs.) at 1:1 PEG400-ctDNA monomer to nucleotide ratio. The experiment was carried out at room temperature 22 ºC, with prior vortex stirrer of biocomplex for 10 min to improve homogeneity of sample. The results showed that the binding reaction of PEG400-ctDNA proceeds rapidly through DNA base pairs and phosphate DNA backbone, and complexation was reached after a maximum 1hr after mixing PEG400 and ctDNA at 1:1 ratio. FTIR spectroscopy results suggest that PEG400 binds with ctDNA by weak to moderate biocomplexes formation, with both hydrophilic and hydrophobic contact through DNA base pairs, with minor binding preference towards phosphate backbone of DNA helix. The mode of interaction most likely referred to an interaction through outside groove binding or electrostatic binding modes. FTIR highlighted the significant effect of incubation time on the stable biocomplexes of non-ionic PEG400 and ctDNA. Moreover, FTIR spectroscopy technique was rapid, showed good stability, and is a valuable tool for studying the biological properties of biocomplexes of PEG400 and ctDNA.