Takiko Tateishi - Academia.edu (original) (raw)
Uploads
Papers by Takiko Tateishi
International journal of analytical bio-science, Jun 1, 2018
Ethanolamine (Etn) contributes to the generation of phosphatidyl ethanolamine (PE), a membrane ph... more Ethanolamine (Etn) contributes to the generation of phosphatidyl ethanolamine (PE), a membrane phosphatide. A study reported that urine Etn was increased in newborns with Zellweger syndrome, a congenital metabolic disease. And more, recently, a metabolomics study, using mass spectrometry, reported that the saliva Etn of pancreatic cancer patients was significantly increased compared to that of normal. However, these have not yet become clear clinically. Baba et al. reported that the reference interval of Etn in healthy volunteer’s serum is 11.84 ± 4.15 μmol/L. However, the clinical significance of Etn in adult blood was not clarified. Etn is measured conventionally by high performance liquid chromatography (HPLC) and capillary electrophoresis-mass spectrometry. These methods, however, have drawbacks including complicated preprocessing operations, and wasting of measuring time and introduction and maintenance costs. Therefore, the aim of this study was to develop a simple, rapid enzy...
Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, 2019
BackgroundThe glycerol-3-phosphate (GPO)-peroxidase (POD) chromogenic method is one of the most w... more BackgroundThe glycerol-3-phosphate (GPO)-peroxidase (POD) chromogenic method is one of the most widely used methods to assay triglycerides. However, it is well known that peroxidase is affected by reducing agents, and recently, it has been reported that some materials affect its activity. Moreover, there is a high possibility of non-specific reaction, as the method uses many enzymes. Against this background, we developed a simpler assay method for triglycerides without using peroxidase.MethodsTriglycerides were hydrolysed to glycerol and fatty acids by lipoprotein lipase followed by the oxidation of glycerol to dihydroxyacetone with simultaneous production of NADH by glycerol dehydrogenase. To overcome incomplete conversion of glycerol to dihydroxyacetone by glycerol dehydrogenase at equilibrium, we added 2-(2-methoxy-4-nitrophenyl)-3–(4-nitrophenyl)-5–(2,4-disulfophenyl)-2 H-tetrazolium monosodium salt (WST-8) to the reaction mixture to remove NADH, allowing the reaction to complet...
Clinica chimica acta; international journal of clinical chemistry, Jan 16, 2015
K(+) has important physiological functions. K(+) concentrations in serum are generally determined... more K(+) has important physiological functions. K(+) concentrations in serum are generally determined using ion-selective electrodes (ISE), though measurement using reagents in aqueous medium is also useful. K(+) concentrations were measured using recombinant inosine 5'-monophosphate dehydrogenase (IMPDH), which was activated only by K(+) and NH4(+). Exogenous and endogenous NH4(+) were eliminated using glutamine synthase. Regression analysis of the enzymatic assay (y) vs. the ISE method (x) gave the following relation: y = 1.03x + 0.09 (n = 54, Sy,x = 0.06 mmol/l). The linear range was up to 12 mmol/l when 1 U/ml IMPDH was used. Advantages of the proposed assay method are: (i) the measured range is wider than that of existing enzymatic methods; (ii) the conditions for K(+) determination can be maintained constant, regardless of the amount of NH4(+) in the analyte and reagents; and (iii) the elimination system is simpler because the recombinant IMPDH is stimulated by only K(+) and N...
Annals of clinical biochemistry, Jan 2, 2015
Tamm-Horsfall protein (THP, also known as Uromodulin) is the most abundant urinary protein in hea... more Tamm-Horsfall protein (THP, also known as Uromodulin) is the most abundant urinary protein in healthy individuals. Since initially characterized by Tamm and Horsfall, amount of urinary excretion and structural mutations of THP are associated with kidney diseases. However, currently available assays for THP, which are mainly enzyme-linked immunosorbent assay (ELISA)-based, suffer from poor reproducibility and might give false negative results. We developed a novel, quantitative assay for THP using reversed-phase high-performance liquid chromatography (HPLC). A precipitation pretreatment avoided urine matrix interference and excessive sample dilution. HPLC optimization based on polarity allowed excellent separation of THP from other major urine components. Our method exhibited high precision (based on the relative standard deviations of intraday (⩽2.77%) and interday (⩽5.35%) repetitions). The THP recovery rate was 100.0-104.2%. The mean THP level in 25 healthy individuals was 31.6 ± ...
Rinsho byori. The Japanese journal of clinical pathology, 2005
Recently nutrition support team (NST) has been established for the purpose of prevention of compl... more Recently nutrition support team (NST) has been established for the purpose of prevention of complications which are caused by nutrition disorders and reduction of the medical expenses. Although physical examinations and blood biochemical data had been used as the indexes evaluating nutritional of patients, they were not suitable for the evaluation for the short-term in-patient. On the contrary, serum albumin (ALB) has been wildly used as a nutritional marker. However, it is impossible to evaluate nutrition state for the short-term in-patient and acute phase disease patient accurately, because the plasma half-life is 21 days and it takes long time to detect the change in nutritional state by its value. Rapid turnover proteins (RTP), whose plasma half-life is shorter, has paid attention to evaluate nutritional state for the short-term in-patients and acute phase disease patients. Although, prognostic inflammatory and nutritional index (PINI) was considered as a useful maker for evalua...
The Journal of rheumatology, 2006
To determine the possibility that T cells represent a potential target for therapy in AA amyloido... more To determine the possibility that T cells represent a potential target for therapy in AA amyloidosis. AA amyloidosis was induced in C3H/HeN mice by concomitant administration of AgNO3 and amyloid-enhancing factor (AEF). Mice injected with AgNO3 and AEF received intraperitoneal injections of FK506 (2-200 microg/day). The degree of splenic amyloid deposition was determined by Congo red staining. Serum amyloid A (SAA), interleukin 1beta (IL-1beta), IL-6, and tumor necrosis factor-a concentrations were measured by ELISA. AA amyloidosis was also induced in ICR mice by injection of Freund's complete adjuvant (FCA) and Mycobacterium butyricum without AEF. ICR mice injected with FCA and M. butyricum also received intraperitoneal injections of FK506 (200 microg/day) to eliminate the possibility that FK506 action might depend upon AEF activity in the amyloid formation. Amyloid deposition was also induced with and without AEF in severe combined immunodeficient (SCID) mice and nude mice to ...
International journal of analytical bio-science, Jun 1, 2018
Ethanolamine (Etn) contributes to the generation of phosphatidyl ethanolamine (PE), a membrane ph... more Ethanolamine (Etn) contributes to the generation of phosphatidyl ethanolamine (PE), a membrane phosphatide. A study reported that urine Etn was increased in newborns with Zellweger syndrome, a congenital metabolic disease. And more, recently, a metabolomics study, using mass spectrometry, reported that the saliva Etn of pancreatic cancer patients was significantly increased compared to that of normal. However, these have not yet become clear clinically. Baba et al. reported that the reference interval of Etn in healthy volunteer’s serum is 11.84 ± 4.15 μmol/L. However, the clinical significance of Etn in adult blood was not clarified. Etn is measured conventionally by high performance liquid chromatography (HPLC) and capillary electrophoresis-mass spectrometry. These methods, however, have drawbacks including complicated preprocessing operations, and wasting of measuring time and introduction and maintenance costs. Therefore, the aim of this study was to develop a simple, rapid enzy...
Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, 2019
BackgroundThe glycerol-3-phosphate (GPO)-peroxidase (POD) chromogenic method is one of the most w... more BackgroundThe glycerol-3-phosphate (GPO)-peroxidase (POD) chromogenic method is one of the most widely used methods to assay triglycerides. However, it is well known that peroxidase is affected by reducing agents, and recently, it has been reported that some materials affect its activity. Moreover, there is a high possibility of non-specific reaction, as the method uses many enzymes. Against this background, we developed a simpler assay method for triglycerides without using peroxidase.MethodsTriglycerides were hydrolysed to glycerol and fatty acids by lipoprotein lipase followed by the oxidation of glycerol to dihydroxyacetone with simultaneous production of NADH by glycerol dehydrogenase. To overcome incomplete conversion of glycerol to dihydroxyacetone by glycerol dehydrogenase at equilibrium, we added 2-(2-methoxy-4-nitrophenyl)-3–(4-nitrophenyl)-5–(2,4-disulfophenyl)-2 H-tetrazolium monosodium salt (WST-8) to the reaction mixture to remove NADH, allowing the reaction to complet...
Clinica chimica acta; international journal of clinical chemistry, Jan 16, 2015
K(+) has important physiological functions. K(+) concentrations in serum are generally determined... more K(+) has important physiological functions. K(+) concentrations in serum are generally determined using ion-selective electrodes (ISE), though measurement using reagents in aqueous medium is also useful. K(+) concentrations were measured using recombinant inosine 5'-monophosphate dehydrogenase (IMPDH), which was activated only by K(+) and NH4(+). Exogenous and endogenous NH4(+) were eliminated using glutamine synthase. Regression analysis of the enzymatic assay (y) vs. the ISE method (x) gave the following relation: y = 1.03x + 0.09 (n = 54, Sy,x = 0.06 mmol/l). The linear range was up to 12 mmol/l when 1 U/ml IMPDH was used. Advantages of the proposed assay method are: (i) the measured range is wider than that of existing enzymatic methods; (ii) the conditions for K(+) determination can be maintained constant, regardless of the amount of NH4(+) in the analyte and reagents; and (iii) the elimination system is simpler because the recombinant IMPDH is stimulated by only K(+) and N...
Annals of clinical biochemistry, Jan 2, 2015
Tamm-Horsfall protein (THP, also known as Uromodulin) is the most abundant urinary protein in hea... more Tamm-Horsfall protein (THP, also known as Uromodulin) is the most abundant urinary protein in healthy individuals. Since initially characterized by Tamm and Horsfall, amount of urinary excretion and structural mutations of THP are associated with kidney diseases. However, currently available assays for THP, which are mainly enzyme-linked immunosorbent assay (ELISA)-based, suffer from poor reproducibility and might give false negative results. We developed a novel, quantitative assay for THP using reversed-phase high-performance liquid chromatography (HPLC). A precipitation pretreatment avoided urine matrix interference and excessive sample dilution. HPLC optimization based on polarity allowed excellent separation of THP from other major urine components. Our method exhibited high precision (based on the relative standard deviations of intraday (⩽2.77%) and interday (⩽5.35%) repetitions). The THP recovery rate was 100.0-104.2%. The mean THP level in 25 healthy individuals was 31.6 ± ...
Rinsho byori. The Japanese journal of clinical pathology, 2005
Recently nutrition support team (NST) has been established for the purpose of prevention of compl... more Recently nutrition support team (NST) has been established for the purpose of prevention of complications which are caused by nutrition disorders and reduction of the medical expenses. Although physical examinations and blood biochemical data had been used as the indexes evaluating nutritional of patients, they were not suitable for the evaluation for the short-term in-patient. On the contrary, serum albumin (ALB) has been wildly used as a nutritional marker. However, it is impossible to evaluate nutrition state for the short-term in-patient and acute phase disease patient accurately, because the plasma half-life is 21 days and it takes long time to detect the change in nutritional state by its value. Rapid turnover proteins (RTP), whose plasma half-life is shorter, has paid attention to evaluate nutritional state for the short-term in-patients and acute phase disease patients. Although, prognostic inflammatory and nutritional index (PINI) was considered as a useful maker for evalua...
The Journal of rheumatology, 2006
To determine the possibility that T cells represent a potential target for therapy in AA amyloido... more To determine the possibility that T cells represent a potential target for therapy in AA amyloidosis. AA amyloidosis was induced in C3H/HeN mice by concomitant administration of AgNO3 and amyloid-enhancing factor (AEF). Mice injected with AgNO3 and AEF received intraperitoneal injections of FK506 (2-200 microg/day). The degree of splenic amyloid deposition was determined by Congo red staining. Serum amyloid A (SAA), interleukin 1beta (IL-1beta), IL-6, and tumor necrosis factor-a concentrations were measured by ELISA. AA amyloidosis was also induced in ICR mice by injection of Freund's complete adjuvant (FCA) and Mycobacterium butyricum without AEF. ICR mice injected with FCA and M. butyricum also received intraperitoneal injections of FK506 (200 microg/day) to eliminate the possibility that FK506 action might depend upon AEF activity in the amyloid formation. Amyloid deposition was also induced with and without AEF in severe combined immunodeficient (SCID) mice and nude mice to ...