Tapani Suortti - Academia.edu (original) (raw)

Papers by Tapani Suortti

Research paper thumbnail of Modification of Phospholipids with Lipases and Phospholipases

Biocatalysis, 1994

ABSTRACT Different commercial lipases and phosphoiipases were studied in the hydrolysis and trans... more ABSTRACT Different commercial lipases and phosphoiipases were studied in the hydrolysis and transesterification of synthetic phosphatidylcholine and soybean lecithin. Wide variations in the lipase and phospholipase activities and in the protein contents of the preparations were observed. The substrate specificity varied between different enzymes. A high degree of hydrolysis of synthetic and soybean phospholipids was achieved with both types of enzymes. Enzymes immobilized on Celite were used in the transesterification of dimyristoyl phosphatidylcholine and oleic acid. The conversions were carried out both without solvent and in the presence of toluene. The amount of modified phosphatidylcholine was measured using HPLC. The highest amount of modified phosphatidylcholine was obtained in solvent-free transesterification. The best results were obtained with Aspergillus niyer lipase.

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Research paper thumbnail of Enzymatic modification of wheat bran arabinoxylans at reduced water content

ABSTRACT 8th European Young Cereal Scientists and Technologists Workshop. Viterbo, Italy, 3 - 5 A... more ABSTRACT 8th European Young Cereal Scientists and Technologists Workshop. Viterbo, Italy, 3 - 5 Aug. 2009

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Research paper thumbnail of Modification of barley starch by α-amylase and pullulanase

Carbohydrate Polymers, 1993

Abstract The hydrolysis of the amylopectin of gelatinized waxy barley starch by Bacillus lichenif... more Abstract The hydrolysis of the amylopectin of gelatinized waxy barley starch by Bacillus licheniformis α-amylase occurred in a non-random way, showing accumulation of smaller-size hydrolysis products. The hydrolysis of barley starch by Bacillus acidopullulyticus pullulanase also began with a rapid decrease in the molecular size of amylopectin, indicating that the enzyme was capable of hydrolyzing the α-1,6-linkages in the middle of the amylopectin molecule.

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Research paper thumbnail of In vitro fermentation of polysaccharides in rye, wheat and oat bran

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Research paper thumbnail of Partial hydrolysis of gelatinized barley and waxy barley starches by alpha-amylase

Food Hydrocolloids, Jul 1, 1996

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Research paper thumbnail of Purification and Characterization ofBacillus acidopullulyticus Pullulanase for Enzymatic Starch Modification

Starch-starke, 1991

The pullulanase (EC 3.2.1.41) of Bacillus acidopullulyticus was purified using anion exchange chr... more The pullulanase (EC 3.2.1.41) of Bacillus acidopullulyticus was purified using anion exchange chromatography and preparative isoelectric focusing. The purified preparation migrated as a single protein band upon SDS gel electrophoresis and its molecular weight was estimated to be 102,000. Two main activity bands having pl-values 5.0 and 5.2 were detected by analytical isoelectric focusing. The enzyme was purified 4-fold with the yield 38% by this two-step purification procedure. The purified pullulanase showed maximal activity at 50°C and pH 5 and was slightly activated by Ca2+. It was stable at 50°C but totally lost its activity at 60oC in one hour. This purified pullulanase efficiently hydrolyzed the α-1,6-glucosidic bonds of pullulan and gelatinized starch. Reinigung und Charakterisierung von Pullulanase aus Bacillus acidopullulyticus fur die enzymatische Starkemodifizierung. Das Enzym Pullulanase aus Bacillus acidopullulyticus wurde durch Anionenaustausch-Chromatographie und praparative isoelektrische Fokussierung gereinigt. Die Praparation des gereinigten Proteins zeigte eine einzige Bande im SDS-Gel; als Molekulargewicht wurde 102000 berechnet. Analytische isoelektrische Fokussierung ergab zwei aktive Hauptbanden mit pl-Werten von 5.0 und 5.2. Das Enzym wurde durch die beschriebene Reinigung in zwei Schritten 4-fach angereichert; die Ausbeute betrug 38%. Die gereinigte Pullulanase zeigte maximale Aktivitat be 50oC und pH 5. Eine leichte Erhohung der Aktivitat durch Ca2Plus; wurde beobachtet. Das Enzym war stabil bei 50oC, verlor jedoch vollig seine Aktivitat nach einstundiger Inkubation bei 60oC. Die gereinigte Pullulanase hydrolysierte sehr effizient α-1,6-glucosidische Bindungen in Pullulan und in verkleisterter Starke.

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Research paper thumbnail of Accessibility of Barley Starch Granules to α-Amylase during Different Phases of Gelatinization

Journal of Cereal Science, Mar 1, 1993

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Research paper thumbnail of alfa-Amylolysis of barley starch

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Research paper thumbnail of High molecular weight starch hydrolysis products by alpha-amylase and pullulanase

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Research paper thumbnail of Enzymes acting on fibre surfaces: Analytical challenges

ABSTRACT COST Action E41: Analytical tools with applications for wood and pulping chemistry. Rome... more ABSTRACT COST Action E41: Analytical tools with applications for wood and pulping chemistry. Rome, Italy, 7-8 May 2007

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Research paper thumbnail of Re-evaluation of RI detector for the lipid and phospholipid analysis

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Research paper thumbnail of Enzymatic modification of lecithins

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Research paper thumbnail of Properties and flocculation efficiency of cationized biopolymers and their applicability in papermaking and in conditioning of pulp and paper sludge

Bioresources, Jun 8, 2011

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Research paper thumbnail of High molecular weight starch hydrolysis products by alpha-amylase and pullulanase

44th Starch Convention, 1993

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Research paper thumbnail of Common features and interesting differences in transcriptional responses to secretion stress in the fungi and -0

<b>Copyright information:</b>Taken from "Common features and interesting differe... more <b>Copyright information:</b>Taken from "Common features and interesting differences in transcriptional responses to secretion stress in the fungi and "BMC Genomics 2006;7():32-32.Published online 22 Feb 2006PMCID:PMC1397821.Copyright © 2006 Arvas et al; licensee BioMed Central Ltd.rom 174 different stress conditions were collected from published articles. The expression level values of individual genes are shown in or and the median of the gene group in and the standard deviation of the whole experiment in . "oreUPR", genes showing significant up regulation in "" experiment set against both the "" and "experiment sets. "Travers UPR", genes defined as UPR genes and genes defined as UPR model genes in by [2]. "UPR Dependent Up", genes showing significant up regulation only in "" set against "" set. "UPR Dependent Down", genes showing significant down regulation only in "" against "" set. "UPR Independent Up", genes showing significant up regulation only in "" against "" set. "UPR Independent Down", genes showing significant down regulation only in "" against "" set. The stress conditions were divided in sets as follows: IRE1 and HAC1 deletion strains treated with DTT or tunicamycin, used as UPR dependent reference set in this study [2, 3]; reference set, used as UPR independent reference in this study [17, 18]; cultures treated with DTT or tunicamycin or recombinant strains producing a secreted heterologous protein, used as UPR conditions in this study [2, 3, 17-19, 69]; DTT treatment, 4 h time series [17]; Cdtreatment, 2 h time series and met4 deletion [48]; strains deleted in cell wall genes [49]; . methyl methanesulfonate, 3-aminotriazole and amino acid depletion treatments and GCN4 constitutive and GCN4 deletion strains [35]; 1 h time series after heat shock from 25°C to 37°C, [17]; 1, 5 h time series after temperature shift from 37°C to 25°C, [17]; 1, 5 h time series after mild heat shock of 29°C to 33°C [17]; . mild heat shock with different osmolarities, like previous but with sorbitol treatments [17]; diff [...]

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Research paper thumbnail of Re-evaluation of RI detector for the lipid and phospholipid analysis

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Research paper thumbnail of Improving oat b-glucan content by biotechnological methods

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Research paper thumbnail of The Use of Xylanolytic Enzymes in Processing of Cereals

Developments in Food Engineering, 1994

Treatment of whole meal rye flour slurries with pure endoxylanase caused fragmentation of the cel... more Treatment of whole meal rye flour slurries with pure endoxylanase caused fragmentation of the cell walls and increased extraction of the pentosans. Due to the heterogenous structure of rye cell walls also s-glucanase and protease were needed for their complete degradation. About 30 % of the rye flour pentosans remained insoluble even after treatment with a high dosage of Trichoderma reesei culture filtrate rich with xylanolytic enzymes and s-glucanases. Pure xylanases effectively reduced the viscosity of polymeric rye flour water-soluble polysaccharides.

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Research paper thumbnail of The effects of a xylanase and α β-glucanase from Trichoderma reesei on the non-starch polysaccharides of whole meal rye slurry

Journal of Cereal Science, 1995

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Research paper thumbnail of The isolation of lactic acid bacteria from human colonic biopsies after enrichment on lactose derivatives and rye arabinoxylo-oligosaccharides

Food Microbiology, 2000

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Research paper thumbnail of Modification of Phospholipids with Lipases and Phospholipases

Biocatalysis, 1994

ABSTRACT Different commercial lipases and phosphoiipases were studied in the hydrolysis and trans... more ABSTRACT Different commercial lipases and phosphoiipases were studied in the hydrolysis and transesterification of synthetic phosphatidylcholine and soybean lecithin. Wide variations in the lipase and phospholipase activities and in the protein contents of the preparations were observed. The substrate specificity varied between different enzymes. A high degree of hydrolysis of synthetic and soybean phospholipids was achieved with both types of enzymes. Enzymes immobilized on Celite were used in the transesterification of dimyristoyl phosphatidylcholine and oleic acid. The conversions were carried out both without solvent and in the presence of toluene. The amount of modified phosphatidylcholine was measured using HPLC. The highest amount of modified phosphatidylcholine was obtained in solvent-free transesterification. The best results were obtained with Aspergillus niyer lipase.

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Research paper thumbnail of Enzymatic modification of wheat bran arabinoxylans at reduced water content

ABSTRACT 8th European Young Cereal Scientists and Technologists Workshop. Viterbo, Italy, 3 - 5 A... more ABSTRACT 8th European Young Cereal Scientists and Technologists Workshop. Viterbo, Italy, 3 - 5 Aug. 2009

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Research paper thumbnail of Modification of barley starch by α-amylase and pullulanase

Carbohydrate Polymers, 1993

Abstract The hydrolysis of the amylopectin of gelatinized waxy barley starch by Bacillus lichenif... more Abstract The hydrolysis of the amylopectin of gelatinized waxy barley starch by Bacillus licheniformis α-amylase occurred in a non-random way, showing accumulation of smaller-size hydrolysis products. The hydrolysis of barley starch by Bacillus acidopullulyticus pullulanase also began with a rapid decrease in the molecular size of amylopectin, indicating that the enzyme was capable of hydrolyzing the α-1,6-linkages in the middle of the amylopectin molecule.

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Research paper thumbnail of In vitro fermentation of polysaccharides in rye, wheat and oat bran

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Partial hydrolysis of gelatinized barley and waxy barley starches by alpha-amylase

Food Hydrocolloids, Jul 1, 1996

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Research paper thumbnail of Purification and Characterization ofBacillus acidopullulyticus Pullulanase for Enzymatic Starch Modification

Starch-starke, 1991

The pullulanase (EC 3.2.1.41) of Bacillus acidopullulyticus was purified using anion exchange chr... more The pullulanase (EC 3.2.1.41) of Bacillus acidopullulyticus was purified using anion exchange chromatography and preparative isoelectric focusing. The purified preparation migrated as a single protein band upon SDS gel electrophoresis and its molecular weight was estimated to be 102,000. Two main activity bands having pl-values 5.0 and 5.2 were detected by analytical isoelectric focusing. The enzyme was purified 4-fold with the yield 38% by this two-step purification procedure. The purified pullulanase showed maximal activity at 50°C and pH 5 and was slightly activated by Ca2+. It was stable at 50°C but totally lost its activity at 60oC in one hour. This purified pullulanase efficiently hydrolyzed the α-1,6-glucosidic bonds of pullulan and gelatinized starch. Reinigung und Charakterisierung von Pullulanase aus Bacillus acidopullulyticus fur die enzymatische Starkemodifizierung. Das Enzym Pullulanase aus Bacillus acidopullulyticus wurde durch Anionenaustausch-Chromatographie und praparative isoelektrische Fokussierung gereinigt. Die Praparation des gereinigten Proteins zeigte eine einzige Bande im SDS-Gel; als Molekulargewicht wurde 102000 berechnet. Analytische isoelektrische Fokussierung ergab zwei aktive Hauptbanden mit pl-Werten von 5.0 und 5.2. Das Enzym wurde durch die beschriebene Reinigung in zwei Schritten 4-fach angereichert; die Ausbeute betrug 38%. Die gereinigte Pullulanase zeigte maximale Aktivitat be 50oC und pH 5. Eine leichte Erhohung der Aktivitat durch Ca2Plus; wurde beobachtet. Das Enzym war stabil bei 50oC, verlor jedoch vollig seine Aktivitat nach einstundiger Inkubation bei 60oC. Die gereinigte Pullulanase hydrolysierte sehr effizient α-1,6-glucosidische Bindungen in Pullulan und in verkleisterter Starke.

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Research paper thumbnail of Accessibility of Barley Starch Granules to α-Amylase during Different Phases of Gelatinization

Journal of Cereal Science, Mar 1, 1993

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Research paper thumbnail of alfa-Amylolysis of barley starch

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Research paper thumbnail of High molecular weight starch hydrolysis products by alpha-amylase and pullulanase

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Enzymes acting on fibre surfaces: Analytical challenges

ABSTRACT COST Action E41: Analytical tools with applications for wood and pulping chemistry. Rome... more ABSTRACT COST Action E41: Analytical tools with applications for wood and pulping chemistry. Rome, Italy, 7-8 May 2007

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Research paper thumbnail of Re-evaluation of RI detector for the lipid and phospholipid analysis

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Research paper thumbnail of Enzymatic modification of lecithins

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Research paper thumbnail of Properties and flocculation efficiency of cationized biopolymers and their applicability in papermaking and in conditioning of pulp and paper sludge

Bioresources, Jun 8, 2011

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Research paper thumbnail of High molecular weight starch hydrolysis products by alpha-amylase and pullulanase

44th Starch Convention, 1993

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Research paper thumbnail of Common features and interesting differences in transcriptional responses to secretion stress in the fungi and -0

<b>Copyright information:</b>Taken from "Common features and interesting differe... more <b>Copyright information:</b>Taken from "Common features and interesting differences in transcriptional responses to secretion stress in the fungi and "BMC Genomics 2006;7():32-32.Published online 22 Feb 2006PMCID:PMC1397821.Copyright © 2006 Arvas et al; licensee BioMed Central Ltd.rom 174 different stress conditions were collected from published articles. The expression level values of individual genes are shown in or and the median of the gene group in and the standard deviation of the whole experiment in . "oreUPR", genes showing significant up regulation in "" experiment set against both the "" and "experiment sets. "Travers UPR", genes defined as UPR genes and genes defined as UPR model genes in by [2]. "UPR Dependent Up", genes showing significant up regulation only in "" set against "" set. "UPR Dependent Down", genes showing significant down regulation only in "" against "" set. "UPR Independent Up", genes showing significant up regulation only in "" against "" set. "UPR Independent Down", genes showing significant down regulation only in "" against "" set. The stress conditions were divided in sets as follows: IRE1 and HAC1 deletion strains treated with DTT or tunicamycin, used as UPR dependent reference set in this study [2, 3]; reference set, used as UPR independent reference in this study [17, 18]; cultures treated with DTT or tunicamycin or recombinant strains producing a secreted heterologous protein, used as UPR conditions in this study [2, 3, 17-19, 69]; DTT treatment, 4 h time series [17]; Cdtreatment, 2 h time series and met4 deletion [48]; strains deleted in cell wall genes [49]; . methyl methanesulfonate, 3-aminotriazole and amino acid depletion treatments and GCN4 constitutive and GCN4 deletion strains [35]; 1 h time series after heat shock from 25°C to 37°C, [17]; 1, 5 h time series after temperature shift from 37°C to 25°C, [17]; 1, 5 h time series after mild heat shock of 29°C to 33°C [17]; . mild heat shock with different osmolarities, like previous but with sorbitol treatments [17]; diff [...]

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Research paper thumbnail of Re-evaluation of RI detector for the lipid and phospholipid analysis

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Research paper thumbnail of Improving oat b-glucan content by biotechnological methods

Bookmarks Related papers MentionsView impact

Research paper thumbnail of The Use of Xylanolytic Enzymes in Processing of Cereals

Developments in Food Engineering, 1994

Treatment of whole meal rye flour slurries with pure endoxylanase caused fragmentation of the cel... more Treatment of whole meal rye flour slurries with pure endoxylanase caused fragmentation of the cell walls and increased extraction of the pentosans. Due to the heterogenous structure of rye cell walls also s-glucanase and protease were needed for their complete degradation. About 30 % of the rye flour pentosans remained insoluble even after treatment with a high dosage of Trichoderma reesei culture filtrate rich with xylanolytic enzymes and s-glucanases. Pure xylanases effectively reduced the viscosity of polymeric rye flour water-soluble polysaccharides.

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Research paper thumbnail of The effects of a xylanase and α β-glucanase from Trichoderma reesei on the non-starch polysaccharides of whole meal rye slurry

Journal of Cereal Science, 1995

Bookmarks Related papers MentionsView impact

Research paper thumbnail of The isolation of lactic acid bacteria from human colonic biopsies after enrichment on lactose derivatives and rye arabinoxylo-oligosaccharides

Food Microbiology, 2000

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