Tri Hadi - Academia.edu (original) (raw)
Papers by Tri Hadi
Microplastics are categorized as less than 5 mm in length-sized plastics. Lombok is located in In... more Microplastics are categorized as less than 5 mm in length-sized plastics. Lombok is located in Indonesia, part of the coral triangle region, and one of the out flow locations of the Indonesian Through Flow (ITF). There is likelihood that microplastics would flow across the ITF and would be accumulated in the coral reef ecosystem, especially in biota and sediment. To improve the knowledge of microplastics pollution in Indonesia, we analyzed sediment samples from 10 stations at coral reef habitats in Sekotong, Lombok-Indonesia. The microplastics concentration in Sekotong varied from 35 to 77 particles per-kg, with average 48.3±13.98 (SD) particles per-kg, found in all ten sampling location. The highest concentration was found in the southwest of Gili Gede Island (77 particles per-kg). All of the microplastics collected were foam (41.20%), fragment (32.51%), granule (22.77%) and fiber (3.52%). Microplastic with size more than 1000 μm found at most, followed by size range of 500-1000 µm, 200-500 µm and particle size less than 200 µm in length. Polystyrene was the most abundant type of plastic polymer identified, followed by polyethylene and polypropylene. This type of polymers indicates that the primary source of microplastics in the Sekotong's coral reef sediment was from the usage of styrofoam, food and beverage packages, also fishing devices. It is strongly suggested that the management of plastic waste to be improved and it is essential to develop an environmentally friendly substance to replace plastics in near future.
Uji pendahuluan bioaktivitas dan identifikasi gugus fungsi metabolit sekunder ekstrak dalam metan... more Uji pendahuluan bioaktivitas dan identifikasi gugus fungsi metabolit sekunder ekstrak dalam metanol, n-heksan and metilen klorida dari spons Agelas nakamurai Hoshino, asal pulau Kapoposang, kepulauan Spermonde telah dilakukan. Juga test bioaktivitas dilakukan menggunakan Artemia salina (LC50) untuk mendapatkan konsentrasi letalnya. Hasilnya LC50 adalah 187.932 ppm yang menunjukkan potensi ekstrak sebagai obat anti kanker. Identifikasi komponen dengan reagensia spesifik seperti Lieberman- Burchard, Mayer, Wagner, Dragendorff dan Salkowski ditambah hasil analisis FTIR menunjukkan kandungan komponen ekstrak seperti alkaloid, steroid and terpenoid. Karakteristik pita serapan alkaloid adalah for alkaloid adalah stretching NH at 3600 to 3400 cm-1 dan stretching pendukung C-N at 1250 to 1930 cm-1. Komponen spesifik untuk Agelas nakamurai adalah substitusi bromo dalam alkaloid yang ditandai oleh munculnya pita serapan C-Br medium pada 760 to 745 cm-1.
Biodiversity, 2018
The dynamics of coral reef benthic and fish communities and their relationship within the Batam a... more The dynamics of coral reef benthic and fish communities and their relationship within the Batam and Natuna Islands were investigated in 2004, 2007, 2010 and 2013. Hard coral covers declined at both locations (Batam by 18.69% and Natuna by 16.50%) in 2013, after which a mass bleaching event occurred in 2010. This dramatically affected fish abundance, given that there was a decline of 39.18 individuals per transect in Batam but strangely there was an increase by 47.36 individuals per transect for Natuna. This increase might be related to the isolated location of Natuna, which prevented fishes from migration, especially juveniles. In the same year, the benthic compositions in both locations changed from Acroporidae dominated reefs to become less complex reefs. Following this, the fish composition became less diverse for Batam but in reverse for Natuna. The changes in benthic communities correlate significantly with fish functional group diversity for Batam and the abundance for Natuna. These differences might be related to geographical conditions and human impacts which are different between the locations, such as being surrounded by group of islands or open sea, and fishing activities. The communities are dynamic spatially and temporally as a result of natural conditions of the environment and human activities.
Biodiversitas Journal of Biological Diversity, 2018
Hadi TA, Hafizt M, Hadiyanto, Budiyanto A, Siringoringo RM. 2018. Shallow water sponges along the... more Hadi TA, Hafizt M, Hadiyanto, Budiyanto A, Siringoringo RM. 2018. Shallow water sponges along the south coast of Java, Indonesia. Biodiversitas 19: 485-493. Sponges are the most diverse benthic filter feeders, occupying many different types of marine habitat. The south coast of Java is one such marine habitat, very exposed to the open sea. This study investigated the sponge diversity as well as their morphological characters across the south coast of Java. The observations were carried out from 2011 to 2016 in four different locations, including Pamang Peuk, Gunungkidul, Prigi Bay and Bayuwangi. The study found 96 sponge species, from 15 orders, and described them in terms of nine morphological characters. The most common species included Spheciospongia inconstans, Stylissa massa, Callyspongia sp. and Cinachyrella australiensis, while the most common growth forms were massive and encrusting, accounting respectively for 34.4% and 28.1% of the total number of species. There was a sign...
ILMU KELAUTAN: Indonesian Journal of Marine Sciences, 2012
Perairan Kendari merupakan bagian dari wilayah segitiga karang dunia atau lebih dikenal dengan ka... more Perairan Kendari merupakan bagian dari wilayah segitiga karang dunia atau lebih dikenal dengan kawasan Coral Triangle Initiative yang memiliki keragaman karang yang sangat tinggi. Daerah pesisir dan pulau-pulau kecil dilokasi ini mempunyai potensi yang sangat baik bagi kehidupan manusia, namun pada beberapa lokasi terdapat beberapa aktivitas penambangan di darat, yang mana sedikit banyak pasti berdampak bagi kehidupan ekosistem di daerah pantai. Pengamatan terhadap komunitas karang dilakukan di 5 stasiun dengan mengunakan metode transek garis (Line transect). Tujuan pengamatan untuk mengetahui Kondisi terumbukarang, biodiversitas karang. Dari hasil pengamatan menunjukkan bahwa secara umum kondisi karang masih dikategorikan baik dengan rerata persentase tutupan karang 60,25 % (SE 7,08). Persentase tutupan karang hidup mulai dari 42,90-82,60 %. Persentase karang tertinggi berada di Teluk Wawobatu (ST. 1) Sedangkan yang paling rendah yaitu di patch reef Tukalanggara, Gosong Timur (ST. ...
HAYATI Journal of Biosciences, 2017
There has been emerging evidence that the bacteria associated with marine sponges are the key pro... more There has been emerging evidence that the bacteria associated with marine sponges are the key producers of many complex bioactive compounds. The as-yet uncultured candidate bacterial genus "Candidatus Entotheonella" of the marine sponge Theonella swinhoei from Japan have recently been recognized as the source of numerous pharmacologically relevant polyketides and modified peptides, as previously reported by the Piel group (Wilson et al. 2014). This work reported the presence of "Candidatus Entotheonella sp." in the highly complex microbiome of an Indonesian marine sponge from Kapoposang Island, South Sulawesi. We further identified the Kapoposang sponge specimen used in this work as Rhabdastrella sp. based on the integrated morphological, histological, and cytochrome oxidase subunit I (COI) gene analyses. To detect the polyketide biosynthetic machinery called type I polyketide synthase (PKS) in this Indonesian Rhabdastrella sp., we amplified and cloned the ketosynthase-encoding DNA regions of approximately 700 bp from the uncultured sponge's microbiome. Further sequencing and analysis of several randomly chosen clones indicated that all of them are mostly likely involved in the biosynthesis of methyl-branched fatty acids. However, employing a PKS-targeting primer designed in this work led to the isolation of four positive clones. BlastX search and subsequent phylogenetic analysis showed that one of the positive clones, designed as RGK32, displayed high homology with ketosynthase domains of many type I PKS systems and may belong to the subclass cis-AT PKS group.
Journal of Natural Products, 2012
Twenty-one new psammaplysin derivatives (4−24) exhibiting a variety of side chains, as well as si... more Twenty-one new psammaplysin derivatives (4−24) exhibiting a variety of side chains, as well as six previously known psammaplysins, were identified from the Indonesian marine sponge Aplysinella strongylata. The double bond on the side chain of the fatty acid-containing psammaplysins was located by GC-MS analysis of the fatty acid methyl esters and their pyrrolidide derivatives. HPLC and Mosher ester studies confirmed that the isolated metabolites possessing a 19-OH substituent were mixtures of diastereomers. Selected compounds (4, 5, 7, 8, 12, 18, and 22) were screened for in vitro activity against chloroquine-sensitive (3D7) P. falciparum malaria parasites. Of the new psammaplysins, 19-hydroxypsammaplysin E (4) showed the best antimalarial activity, with an IC 50 value of 6.4 μM.
Microplastics are categorized as less than 5 mm in length-sized plastics. Lombok is located in In... more Microplastics are categorized as less than 5 mm in length-sized plastics. Lombok is located in Indonesia, part of the coral triangle region, and one of the out flow locations of the Indonesian Through Flow (ITF). There is likelihood that microplastics would flow across the ITF and would be accumulated in the coral reef ecosystem, especially in biota and sediment. To improve the knowledge of microplastics pollution in Indonesia, we analyzed sediment samples from 10 stations at coral reef habitats in Sekotong, Lombok-Indonesia. The microplastics concentration in Sekotong varied from 35 to 77 particles per-kg, with average 48.3±13.98 (SD) particles per-kg, found in all ten sampling location. The highest concentration was found in the southwest of Gili Gede Island (77 particles per-kg). All of the microplastics collected were foam (41.20%), fragment (32.51%), granule (22.77%) and fiber (3.52%). Microplastic with size more than 1000 μm found at most, followed by size range of 500-1000 µm, 200-500 µm and particle size less than 200 µm in length. Polystyrene was the most abundant type of plastic polymer identified, followed by polyethylene and polypropylene. This type of polymers indicates that the primary source of microplastics in the Sekotong's coral reef sediment was from the usage of styrofoam, food and beverage packages, also fishing devices. It is strongly suggested that the management of plastic waste to be improved and it is essential to develop an environmentally friendly substance to replace plastics in near future.
Uji pendahuluan bioaktivitas dan identifikasi gugus fungsi metabolit sekunder ekstrak dalam metan... more Uji pendahuluan bioaktivitas dan identifikasi gugus fungsi metabolit sekunder ekstrak dalam metanol, n-heksan and metilen klorida dari spons Agelas nakamurai Hoshino, asal pulau Kapoposang, kepulauan Spermonde telah dilakukan. Juga test bioaktivitas dilakukan menggunakan Artemia salina (LC50) untuk mendapatkan konsentrasi letalnya. Hasilnya LC50 adalah 187.932 ppm yang menunjukkan potensi ekstrak sebagai obat anti kanker. Identifikasi komponen dengan reagensia spesifik seperti Lieberman- Burchard, Mayer, Wagner, Dragendorff dan Salkowski ditambah hasil analisis FTIR menunjukkan kandungan komponen ekstrak seperti alkaloid, steroid and terpenoid. Karakteristik pita serapan alkaloid adalah for alkaloid adalah stretching NH at 3600 to 3400 cm-1 dan stretching pendukung C-N at 1250 to 1930 cm-1. Komponen spesifik untuk Agelas nakamurai adalah substitusi bromo dalam alkaloid yang ditandai oleh munculnya pita serapan C-Br medium pada 760 to 745 cm-1.
Biodiversity, 2018
The dynamics of coral reef benthic and fish communities and their relationship within the Batam a... more The dynamics of coral reef benthic and fish communities and their relationship within the Batam and Natuna Islands were investigated in 2004, 2007, 2010 and 2013. Hard coral covers declined at both locations (Batam by 18.69% and Natuna by 16.50%) in 2013, after which a mass bleaching event occurred in 2010. This dramatically affected fish abundance, given that there was a decline of 39.18 individuals per transect in Batam but strangely there was an increase by 47.36 individuals per transect for Natuna. This increase might be related to the isolated location of Natuna, which prevented fishes from migration, especially juveniles. In the same year, the benthic compositions in both locations changed from Acroporidae dominated reefs to become less complex reefs. Following this, the fish composition became less diverse for Batam but in reverse for Natuna. The changes in benthic communities correlate significantly with fish functional group diversity for Batam and the abundance for Natuna. These differences might be related to geographical conditions and human impacts which are different between the locations, such as being surrounded by group of islands or open sea, and fishing activities. The communities are dynamic spatially and temporally as a result of natural conditions of the environment and human activities.
Biodiversitas Journal of Biological Diversity, 2018
Hadi TA, Hafizt M, Hadiyanto, Budiyanto A, Siringoringo RM. 2018. Shallow water sponges along the... more Hadi TA, Hafizt M, Hadiyanto, Budiyanto A, Siringoringo RM. 2018. Shallow water sponges along the south coast of Java, Indonesia. Biodiversitas 19: 485-493. Sponges are the most diverse benthic filter feeders, occupying many different types of marine habitat. The south coast of Java is one such marine habitat, very exposed to the open sea. This study investigated the sponge diversity as well as their morphological characters across the south coast of Java. The observations were carried out from 2011 to 2016 in four different locations, including Pamang Peuk, Gunungkidul, Prigi Bay and Bayuwangi. The study found 96 sponge species, from 15 orders, and described them in terms of nine morphological characters. The most common species included Spheciospongia inconstans, Stylissa massa, Callyspongia sp. and Cinachyrella australiensis, while the most common growth forms were massive and encrusting, accounting respectively for 34.4% and 28.1% of the total number of species. There was a sign...
ILMU KELAUTAN: Indonesian Journal of Marine Sciences, 2012
Perairan Kendari merupakan bagian dari wilayah segitiga karang dunia atau lebih dikenal dengan ka... more Perairan Kendari merupakan bagian dari wilayah segitiga karang dunia atau lebih dikenal dengan kawasan Coral Triangle Initiative yang memiliki keragaman karang yang sangat tinggi. Daerah pesisir dan pulau-pulau kecil dilokasi ini mempunyai potensi yang sangat baik bagi kehidupan manusia, namun pada beberapa lokasi terdapat beberapa aktivitas penambangan di darat, yang mana sedikit banyak pasti berdampak bagi kehidupan ekosistem di daerah pantai. Pengamatan terhadap komunitas karang dilakukan di 5 stasiun dengan mengunakan metode transek garis (Line transect). Tujuan pengamatan untuk mengetahui Kondisi terumbukarang, biodiversitas karang. Dari hasil pengamatan menunjukkan bahwa secara umum kondisi karang masih dikategorikan baik dengan rerata persentase tutupan karang 60,25 % (SE 7,08). Persentase tutupan karang hidup mulai dari 42,90-82,60 %. Persentase karang tertinggi berada di Teluk Wawobatu (ST. 1) Sedangkan yang paling rendah yaitu di patch reef Tukalanggara, Gosong Timur (ST. ...
HAYATI Journal of Biosciences, 2017
There has been emerging evidence that the bacteria associated with marine sponges are the key pro... more There has been emerging evidence that the bacteria associated with marine sponges are the key producers of many complex bioactive compounds. The as-yet uncultured candidate bacterial genus "Candidatus Entotheonella" of the marine sponge Theonella swinhoei from Japan have recently been recognized as the source of numerous pharmacologically relevant polyketides and modified peptides, as previously reported by the Piel group (Wilson et al. 2014). This work reported the presence of "Candidatus Entotheonella sp." in the highly complex microbiome of an Indonesian marine sponge from Kapoposang Island, South Sulawesi. We further identified the Kapoposang sponge specimen used in this work as Rhabdastrella sp. based on the integrated morphological, histological, and cytochrome oxidase subunit I (COI) gene analyses. To detect the polyketide biosynthetic machinery called type I polyketide synthase (PKS) in this Indonesian Rhabdastrella sp., we amplified and cloned the ketosynthase-encoding DNA regions of approximately 700 bp from the uncultured sponge's microbiome. Further sequencing and analysis of several randomly chosen clones indicated that all of them are mostly likely involved in the biosynthesis of methyl-branched fatty acids. However, employing a PKS-targeting primer designed in this work led to the isolation of four positive clones. BlastX search and subsequent phylogenetic analysis showed that one of the positive clones, designed as RGK32, displayed high homology with ketosynthase domains of many type I PKS systems and may belong to the subclass cis-AT PKS group.
Journal of Natural Products, 2012
Twenty-one new psammaplysin derivatives (4−24) exhibiting a variety of side chains, as well as si... more Twenty-one new psammaplysin derivatives (4−24) exhibiting a variety of side chains, as well as six previously known psammaplysins, were identified from the Indonesian marine sponge Aplysinella strongylata. The double bond on the side chain of the fatty acid-containing psammaplysins was located by GC-MS analysis of the fatty acid methyl esters and their pyrrolidide derivatives. HPLC and Mosher ester studies confirmed that the isolated metabolites possessing a 19-OH substituent were mixtures of diastereomers. Selected compounds (4, 5, 7, 8, 12, 18, and 22) were screened for in vitro activity against chloroquine-sensitive (3D7) P. falciparum malaria parasites. Of the new psammaplysins, 19-hydroxypsammaplysin E (4) showed the best antimalarial activity, with an IC 50 value of 6.4 μM.