Tristan Rouyer - Academia.edu (original) (raw)
Papers by Tristan Rouyer
Journal of fish biology, Jun 3, 2024
Analytica Chimica Acta, Mar 1, 2019
Various protocols are currently used to study marine lipids, but there is a growing interest in w... more Various protocols are currently used to study marine lipids, but there is a growing interest in working on dry samples that are easier to transport. However, reference protocols are still lacking for dry samples. In order to make recommendations on this use, lipid classes and fatty acids (FA) obtained from six analytical protocols using two different tissue states (dry vs wet) and three extraction methods (automat vs manual potter vs leaving the solvent to work on tissue) were compared. Three dry storage modes of tissue (freezer vs gas nitrogen vs dry room) during one and three months were also compared. These comparisons were made on seven marine species with different lipid profiles, including fishes, crustaceans and mollusks. Lipid classes and FA obtained from wet and dry tissues were similar, but they were affected by the extraction methods. Regardless of tissue state, "Leave to work" methods obtained the highest lipid quantities, followed by manual potter and automat methods (ca. 90% and 80% of "Leave to work" methods, respectively). Linear relationships allowed correction for lipid classes and FA concentrations obtained from different protocols. The repeatability of all protocols still needs to be improved, especially for fish species. Increasing the replicate number for each sample might be an indirect way to improve lipid quantification. Our results show that storing dry tissues in the freezer for more than one month was associated with a decrease in lipids, which is also observed for other storage methods. For qualitative studies of FA (expressed in %), a threemonth storage of dry tissue in freezer did not affect the relative composition of species/tissues with a lipid content below 20% of dry weight.
Avoiding overfishing and ensuring the sustainability of the resource base is of prime importance ... more Avoiding overfishing and ensuring the sustainability of the resource base is of prime importance for fishery management. Current policies predominately rely on aggregate estimates of stock biomass to set total harvest quotas. Since many com-mercially exploited fish stocks ...
With the growing interest in wireless medical implants, efficient and secure intra-body communica... more With the growing interest in wireless medical implants, efficient and secure intra-body communication becomes mandatory. A promising technique is the Galvanic Coupling (GC) communication. This research area is in constant evolution but despite this, currently there is no complete miniaturized transceiver architecture solution proposed in the literature. In this paper, we present the first fully-miniaturized GC transceiver architecture based on off-the-shelf components considering implantability constrains such as size and power consumption. The transmitter consists of a micro-controller, a Direct Digital Synthesizer (DDS) for analog sine wave generation and an amplifier. The receiver is made of a two-stage analog amplification chain. The first stage is a instrumentation amplifier and the second stage is made of a operational amplifier (Op Amp). Two versions of the receiver has been designed with two instrumentation amplifiers. The design has been tested using a phantom, in a on-body to on-body and an implanted to implanted configurations. The proposed architecture is a compromise between implantation constraints (components area of 0.75 cm² and maximum consumption 18 mA) and maximum transmission frequency (800 kHz). The intra-body communication has been validated for various distances (up to 60 cm), four different electrodes relative orientations and in different settings (on body and intra-body).. . .
![Research paper thumbnail of Monitoring small scale fisheries (canoe) trajectories in West Africa: interest for management and research perspective [résumé]](https://attachments.academia-assets.com/111815815/thumbnails/1.jpg)
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Journal of Fish Biology
The Atlantic bluefin tuna (ABFT) is a highly prized species of large pelagic fish. Studies of the... more The Atlantic bluefin tuna (ABFT) is a highly prized species of large pelagic fish. Studies of their environmental physiology may improve understanding and management of their populations, but this is difficult for mature adults because of their large size. Biologging of heart rate holds promise in investigating physiological responses to environmental conditions in free‐swimming fishes but it is very challenging to anesthetize large ABFT for invasive surgery to place a tag in the body cavity near to the heart. We describe a novel method for rapid deployment of a commercially available heart‐rate tag on ABFT, using an atraumatic trocar to implant it in the musculature associated with the cleithrum. We performed three sequential experiments to show that the tagging method (1) is consistently repeatable and reliable, (2) can be used successfully on commercial fishing boats and does not seem to affect fish survival, and (3) is effective for long‐term deployments. In experiment 3, a tag ...
As biodiversity plummets due to anthropogenic disturbances, the conservation of oceanic species i... more As biodiversity plummets due to anthropogenic disturbances, the conservation of oceanic species is made harder by limited knowledge of their distributions and migrations. Indeed, tracking species distributions in the open ocean is particularly challenging due to scarce observations, and the complex and variable nature of the ocean system. In this study, we propose a new method that leverages deep learning, specifically convolutional neural networks (CNNs), to capture spatial features of environmental variables. This novelty eliminates the need to predefine these features before modelling and creates opportunities to discover unexpected correlations. Our aim is to present the results of the first trial of this method in the open oceans, discuss limitations, and provide feedback for future improvements or adjustments.In this case study, we considered 38 taxa which include pelagic fishes, elasmobranchs, marine mammals, as well as marine turtles and birds. We trained a model to make pro...
Fishes
Repeated fish handling may cause stress, which biases experiments and so affects the results. In ... more Repeated fish handling may cause stress, which biases experiments and so affects the results. In order to reduce this, the present study investigates the benefit of using bioimpedance analysis to estimate morphological parameters. Bioimpedance analysis is a non-lethal and integrative electrical measurement that can be used to estimate several kinds of physiological parameters and is used in medicine and ecological studies. In particular, bioimpedance can monitor the individual growth of fish, which is a prerequisite for most biological and ecological studies, as body size is one of the best predictors for numerous life history traits and ecological parameters. After a short review on the use of bioimpedance measurement in medicine and ecology, we illustrate the potential of bioimpedance spectroscopy, as opposed to single frequency measurement, for experimental studies on fish. Using a monolithic four-contact electrode and a cost-effective portable system, we conducted bioimpedance s...
Réponse à la saisine DPMA 13-5548 sur l'analyse des actions nécessaires pour la mise en oeuvre du... more Réponse à la saisine DPMA 13-5548 sur l'analyse des actions nécessaires pour la mise en oeuvre du suivi et de l'évaluation scientifique des plans de gestion Méditerranée.
Votre courrier du 3 juin dernier interroge l'lfremer sur l'évaluation de l'impact du« pêcher-relâ... more Votre courrier du 3 juin dernier interroge l'lfremer sur l'évaluation de l'impact du« pêcher-relâcher» dans le cadre de la pêche de loisir du thon rouge.
D'après www.ifremer.fr/SIH-indices-campagnes Méthode standardisée d'observation des Biohut. © Eco... more D'après www.ifremer.fr/SIH-indices-campagnes Méthode standardisée d'observation des Biohut. © Ecocean Observation de castagnoles (Chromis chromis) autour d'un Biohut.
Environmental Biology of Fishes
report the results of two tagging operations carried out on a commercial purse seiner during two ... more report the results of two tagging operations carried out on a commercial purse seiner during two consecutive years in the spawning ground around the Maltese islands in the Central Mediterranean Sea. During these operations, eight individuals were tagged and the results showed that the larger fish (> 200 cm) undertook large-scale migrations outside the Mediterranean, whereas smaller individuals did not. This study suggests that size might affect the migratory behavior of Atlantic Bluefin Tuna, and underlines the potential of large-scale tagging operations from spawning grounds to address scientific questions having significant management implications.
2017 International Mixed Signals Testing Workshop (IMSTW), 2017
On-chip sine-wave signal generation is widely covered by literature for Built-In-Self Test (BIST)... more On-chip sine-wave signal generation is widely covered by literature for Built-In-Self Test (BIST) or biosensor applications. The objective is to generate pure and robust sinewave signal with minimal hardware resources. An attractive solution consists in combining several digital signals to built this analog sine-wave. The objective of this paper is to give an analytical study of various potential solutions based on digitalbased approaches. Thanks to this study, we prove that technique consisting in setting the phase shifts and various amplitude values of the square-wave signals is the most efficient approach. Moreover, this study allows the selection of the best solution in terms of parameters of the square-wave signals to cancel loworder harmonics of the generated signal.
Journal of fish biology, Jun 3, 2024
Analytica Chimica Acta, Mar 1, 2019
Various protocols are currently used to study marine lipids, but there is a growing interest in w... more Various protocols are currently used to study marine lipids, but there is a growing interest in working on dry samples that are easier to transport. However, reference protocols are still lacking for dry samples. In order to make recommendations on this use, lipid classes and fatty acids (FA) obtained from six analytical protocols using two different tissue states (dry vs wet) and three extraction methods (automat vs manual potter vs leaving the solvent to work on tissue) were compared. Three dry storage modes of tissue (freezer vs gas nitrogen vs dry room) during one and three months were also compared. These comparisons were made on seven marine species with different lipid profiles, including fishes, crustaceans and mollusks. Lipid classes and FA obtained from wet and dry tissues were similar, but they were affected by the extraction methods. Regardless of tissue state, "Leave to work" methods obtained the highest lipid quantities, followed by manual potter and automat methods (ca. 90% and 80% of "Leave to work" methods, respectively). Linear relationships allowed correction for lipid classes and FA concentrations obtained from different protocols. The repeatability of all protocols still needs to be improved, especially for fish species. Increasing the replicate number for each sample might be an indirect way to improve lipid quantification. Our results show that storing dry tissues in the freezer for more than one month was associated with a decrease in lipids, which is also observed for other storage methods. For qualitative studies of FA (expressed in %), a threemonth storage of dry tissue in freezer did not affect the relative composition of species/tissues with a lipid content below 20% of dry weight.
Avoiding overfishing and ensuring the sustainability of the resource base is of prime importance ... more Avoiding overfishing and ensuring the sustainability of the resource base is of prime importance for fishery management. Current policies predominately rely on aggregate estimates of stock biomass to set total harvest quotas. Since many com-mercially exploited fish stocks ...
With the growing interest in wireless medical implants, efficient and secure intra-body communica... more With the growing interest in wireless medical implants, efficient and secure intra-body communication becomes mandatory. A promising technique is the Galvanic Coupling (GC) communication. This research area is in constant evolution but despite this, currently there is no complete miniaturized transceiver architecture solution proposed in the literature. In this paper, we present the first fully-miniaturized GC transceiver architecture based on off-the-shelf components considering implantability constrains such as size and power consumption. The transmitter consists of a micro-controller, a Direct Digital Synthesizer (DDS) for analog sine wave generation and an amplifier. The receiver is made of a two-stage analog amplification chain. The first stage is a instrumentation amplifier and the second stage is made of a operational amplifier (Op Amp). Two versions of the receiver has been designed with two instrumentation amplifiers. The design has been tested using a phantom, in a on-body to on-body and an implanted to implanted configurations. The proposed architecture is a compromise between implantation constraints (components area of 0.75 cm² and maximum consumption 18 mA) and maximum transmission frequency (800 kHz). The intra-body communication has been validated for various distances (up to 60 cm), four different electrodes relative orientations and in different settings (on body and intra-body).. . .
Journal of Fish Biology
The Atlantic bluefin tuna (ABFT) is a highly prized species of large pelagic fish. Studies of the... more The Atlantic bluefin tuna (ABFT) is a highly prized species of large pelagic fish. Studies of their environmental physiology may improve understanding and management of their populations, but this is difficult for mature adults because of their large size. Biologging of heart rate holds promise in investigating physiological responses to environmental conditions in free‐swimming fishes but it is very challenging to anesthetize large ABFT for invasive surgery to place a tag in the body cavity near to the heart. We describe a novel method for rapid deployment of a commercially available heart‐rate tag on ABFT, using an atraumatic trocar to implant it in the musculature associated with the cleithrum. We performed three sequential experiments to show that the tagging method (1) is consistently repeatable and reliable, (2) can be used successfully on commercial fishing boats and does not seem to affect fish survival, and (3) is effective for long‐term deployments. In experiment 3, a tag ...
As biodiversity plummets due to anthropogenic disturbances, the conservation of oceanic species i... more As biodiversity plummets due to anthropogenic disturbances, the conservation of oceanic species is made harder by limited knowledge of their distributions and migrations. Indeed, tracking species distributions in the open ocean is particularly challenging due to scarce observations, and the complex and variable nature of the ocean system. In this study, we propose a new method that leverages deep learning, specifically convolutional neural networks (CNNs), to capture spatial features of environmental variables. This novelty eliminates the need to predefine these features before modelling and creates opportunities to discover unexpected correlations. Our aim is to present the results of the first trial of this method in the open oceans, discuss limitations, and provide feedback for future improvements or adjustments.In this case study, we considered 38 taxa which include pelagic fishes, elasmobranchs, marine mammals, as well as marine turtles and birds. We trained a model to make pro...
Fishes
Repeated fish handling may cause stress, which biases experiments and so affects the results. In ... more Repeated fish handling may cause stress, which biases experiments and so affects the results. In order to reduce this, the present study investigates the benefit of using bioimpedance analysis to estimate morphological parameters. Bioimpedance analysis is a non-lethal and integrative electrical measurement that can be used to estimate several kinds of physiological parameters and is used in medicine and ecological studies. In particular, bioimpedance can monitor the individual growth of fish, which is a prerequisite for most biological and ecological studies, as body size is one of the best predictors for numerous life history traits and ecological parameters. After a short review on the use of bioimpedance measurement in medicine and ecology, we illustrate the potential of bioimpedance spectroscopy, as opposed to single frequency measurement, for experimental studies on fish. Using a monolithic four-contact electrode and a cost-effective portable system, we conducted bioimpedance s...
Réponse à la saisine DPMA 13-5548 sur l'analyse des actions nécessaires pour la mise en oeuvre du... more Réponse à la saisine DPMA 13-5548 sur l'analyse des actions nécessaires pour la mise en oeuvre du suivi et de l'évaluation scientifique des plans de gestion Méditerranée.
Votre courrier du 3 juin dernier interroge l'lfremer sur l'évaluation de l'impact du« pêcher-relâ... more Votre courrier du 3 juin dernier interroge l'lfremer sur l'évaluation de l'impact du« pêcher-relâcher» dans le cadre de la pêche de loisir du thon rouge.
D'après www.ifremer.fr/SIH-indices-campagnes Méthode standardisée d'observation des Biohut. © Eco... more D'après www.ifremer.fr/SIH-indices-campagnes Méthode standardisée d'observation des Biohut. © Ecocean Observation de castagnoles (Chromis chromis) autour d'un Biohut.
Environmental Biology of Fishes
report the results of two tagging operations carried out on a commercial purse seiner during two ... more report the results of two tagging operations carried out on a commercial purse seiner during two consecutive years in the spawning ground around the Maltese islands in the Central Mediterranean Sea. During these operations, eight individuals were tagged and the results showed that the larger fish (> 200 cm) undertook large-scale migrations outside the Mediterranean, whereas smaller individuals did not. This study suggests that size might affect the migratory behavior of Atlantic Bluefin Tuna, and underlines the potential of large-scale tagging operations from spawning grounds to address scientific questions having significant management implications.
2017 International Mixed Signals Testing Workshop (IMSTW), 2017
On-chip sine-wave signal generation is widely covered by literature for Built-In-Self Test (BIST)... more On-chip sine-wave signal generation is widely covered by literature for Built-In-Self Test (BIST) or biosensor applications. The objective is to generate pure and robust sinewave signal with minimal hardware resources. An attractive solution consists in combining several digital signals to built this analog sine-wave. The objective of this paper is to give an analytical study of various potential solutions based on digitalbased approaches. Thanks to this study, we prove that technique consisting in setting the phase shifts and various amplitude values of the square-wave signals is the most efficient approach. Moreover, this study allows the selection of the best solution in terms of parameters of the square-wave signals to cancel loworder harmonics of the generated signal.