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Papers by Tshibangu Damien

South Asian Research Journal of Natural Products, Mar 19, 2018

Aims: To provide knowledge on phytochemistry and bioactivity of Annona reticulata. Study Design: ... more Aims: To provide knowledge on phytochemistry and bioactivity of Annona reticulata. Study Design: Multidisciplinary advanced bibliographic surveys, utilization of ChemBioDraw software package and dissemination of the resulted knowledge.

Annual Research & Review in Biology

Aim: The objective of this study was to determine the phytochemical profile of Senna alata LINN u... more Aim: The objective of this study was to determine the phytochemical profile of Senna alata LINN using chemical screening in solution and thin-layer chromatography, and to assess the antioxidant and anthelmintic activities of the plant’s aqueous extracts. Methodology: All the analyses performed in this study were, respectively, done as described by the standard protocols. These were: the microscopic examination of the plant powders performed using a light microscope, the search for secondary metabolites carried out by chemical screening in solution and by thin-layer chromatography, the determination of the secondary metabolites, and the antioxidant activity carried out by UV-visible spectroscopy and the anthelmintic activity performed by dilution in decreasing order of concentration. Results: Micrographic analysis of the powder of Senna alata revealed the histological elements rich in unicellular covering hairs with a punctate surface and in fragments of palisade parenchyma, with elo...

International Journal of Pathogen Research, 2022

Aims: The objective of this study was to determine the phytochemical profile of Centella asiatica... more Aims: The objective of this study was to determine the phytochemical profile of Centella asiatica, Citrus grandis and Zanthoxylum gilletii leaves, in solution and the thin layer chromatography chemical screening and to evaluate the antibacterial photodynamic activity of the species extracts. Materials and Methods: The search of the secondary metabolites in solution and by thin layer chromatography, the alkaloids and polyphenols extraction were carried out using standard protocols. The antibacterial photodynamic activity was performed using microdilution method in solution and solid culture media. Results: Centella asiatica and Citrus grandis showed the same phytochemical profile, with the presence of alkaloids, terpenoids, steroids and polyphenols such as: flavonoids, free quinones, anthocyanins, leuco-anthocyanins and tannins. Zanthoxylum gilletii extract showed similar profile with the absence of anthocyanins and leucoanthocyanins. The polyphenolic extracts of both Centella asiati...

International Journal of Pharmacy and Chemistry, 2019

It has been reported that more than 80% of the African population uses traditional medicine to pr... more It has been reported that more than 80% of the African population uses traditional medicine to primary healthcare. The aim of the study was to determine the histological elements, the phytochemical composition and evaluate the bioactivities of Ocimum canum in order to promote this plant used in Congolese traditional medicine. The earthworms were collected from the ponds of the secondary forest of Monastère Prieure Notre Dame de l'Assomption in Kinshasa city and the blood used in this study was provided by the Centre de Médecine Mixte et d'Anémie SS in Yolo-Sud quarter, Kinshasa, Democratic Republic of the Congo. The histological elements were determined by microscopic examination while different metabolites were determined using thin layer chromatography. Microscopic examination revealed the presence of crystalliferous fibres, fragment of parenchyma, grandular and non-glandular pluricellular trichomes, spheroidal pollen grains, diacytic stomata, spiral vessels. With regard to chromatographic analysis, phytochemicals identified were anthocyanins, coumarins, flavonoids, terpenoids and iridoids. The extracts from O. canum showed good antihelminthic, antisickling and antioxidant activities related to their phytochemicalsAll these findings constitute a scientific evidence validating the use of this medicinal plant for the management of various ailments in the Democratic Republic of the Congo.

International Journal of Diabetes and Endocrinology, 2019

Non-communicable diseases represent new challenges for the mankind in the fight for health improv... more Non-communicable diseases represent new challenges for the mankind in the fight for health improvement. Among these diseases, diabetes is a major contributor. Diabetes is a serious chronic disease that occurs when the pancreas does not produce enough insulin or when the body is unable to use the insulin produced effectively. This survey was performed in Kwango, Kongo-Central and Kinshasa provinces respectively between October 2016 and September 2017. A questionnaire was administered to the target population in order to collect relevant data. Plant identification was carried out at the herbarium of University of Kinshasa. The findings revealed that the inventoried medicinal flora is made up of 68 species which are divided into 34 families of 58 genera; the leaf is the most commonly used part in the treatment of diabetes while maceration is the most commonly used method of preparation. The use of medicinal plants is reported in almost all age groups from 20-89 years of age but with a predominance among people aged 40-49 years. Trees and phanerophyte species predominate in the flora studied; the majority of users of these medicinal plants have a primary level of education. In-depth phytochemical and pharmacological studies need to be carried out on these plants with a view to their scientific validation in the diabetes management.

Open Journal of Blood Diseases, 2013

Diclipteracolorata C. B. Clarke, Euphorbia hirta L. and Sorghum bicolor (L.) Moench are reported ... more Diclipteracolorata C. B. Clarke, Euphorbia hirta L. and Sorghum bicolor (L.) Moench are reported among plant species used in Congolese traditional medicine to treat Sickle Cell Disease. These medicinal plants have been investigated for their inhibitory effect on the induced sickling process of red blood cells using Emmel's test. Aqueous plant extracts showed good antisickling activity as revealed by the observed normal biconcave form of sickle red blood cells in anoxic conditions. The calculated radius of treated red blood cells by Euphorbia hirta L., Diclipteracolorata C. B. Clarke and Sorghum biocolor (L.) Moench extracts are 3.31 ± 0.55; 3.34 ± 0.53; 3.17 ± 0.52, respectively. Euphorbia hirta L. is the most active with a normalization rate, more than 70%. The chemical screening performed on these plants showed the presence of anthocyanins which were then extracted. The test carried out with anthocyanin extracts showed that these phenolic compounds have a good antisickling activity and, additionally, possess an anti-hemolytic effect on SS blood. This suggests that the evidenced biological activity of these plants would be due to anthocyanins. The results hence obtained justify the use of these plants in Congolese traditional medicine.

International Journal of Life Science Research Archive

The leaves and seeds of Aframomum alboviolaceum and Aframomum angustifolium are specifically used... more The leaves and seeds of Aframomum alboviolaceum and Aframomum angustifolium are specifically used by traditional healers in the Democratic Republic of Congo (DRC) for the treatment of several pathologies. The aim of present study was to determine the microscopic characteristics and phytochemical composition of these species of the genus Aframomum. The microscopic study of these plants revealed the presence of punctate vessels, fiber cluster with calcium oxalate crystals, isolated unicellular hair, fibers, starch grains, spiral vessel fragments, fiber fragments, and indistinct parenchyma fragments. Phytochemical screening revealed several chemical groups such as phenolic acids, flavonoids, coumarins, alkaloids, terpenes, iridoids, saponins etc. The determination of total polyphenols, flavonoids and tannins gave contents ranging from 14.95 ± 0.45 and 63.98 ± 2.04 mgGAE/g, 0.16 ± 0.01 and 10.68 ± 0.32 mgQE/g and between 1.28 ± 0.03 and 28.51 ± 0.56 mg CAE/g respectively. In general, th...

Aromatic compounds are inhibitors of methane biosynthesis in anaerobic treatment of solid wastes ... more Aromatic compounds are inhibitors of methane biosynthesis in anaerobic treatment of solid wastes and industrial efflu-ents. Anaerobic treatment of solid wastes and industrial effluents may be limited by the methanogenic bacteria inhibi-tion exerted by these types of compounds, the production of biogas is not possible and the organic matter contained in the effluent is not reduced. These effluents poured in the nature can be the basis of the pollution. The objective of this study is to evaluate the effect of monosubstituted aromatic compounds functional groups on the methanogenic inhibi-tion. The toxicity to acetoclastic methanogenic bacteria has performed in serum flasks, utilizing digested pig manure as inoculums, by measuring methane production. The nature of aromatic functional groups was observed to have a pro-found effect on the toxicity of the monosubstituted aromatics. Among the monosubstituted aromatic, the chlorobenzene was the most toxic with 50 % of inhibition occurring a...

Drepanoalpha ® is a poly-herbal formula (PHF) used in Democratic Republic of the Congo for the ma... more Drepanoalpha ® is a poly-herbal formula (PHF) used in Democratic Republic of the Congo for the management of Sickle Cell Disease (SCD), a genetic disease due to the presence of hemoglobin S in the blood which could in hypoxia conduct to the formation of tactoids, the leading cause of erythrocyte sickling that plays a key role in the pathophysiology of SCD like vasoocclusion (due to the loss of membrane elasticity) and hemolytic anemia. The aim of the present study was to evaluate the storage time effect on the bioactivity and the secondary metabolites (anthocyanis and flavonoids) and minerals (Fe, Mn, Zn and Cu) profiles of this PHF. Results revealed that flavonoids content of different batches is of the same order of magnitude: the percentage of ratio flavonoids/total polyphenols was 5.3% (batch A); 5.0% (batch B); 4.7% (batch C). However, the anthocyanins content of PHF varies according to the storage time. The percentage of ratio anthocyanis/total polyphenols was 3, 2% for batch ...

American Journal of Essential Oils and Natural Products, 2017

A hydrodistilled oil from the aerial part of Eupatorium africanum Oliv. & Hiern collected from D.... more A hydrodistilled oil from the aerial part of Eupatorium africanum Oliv. & Hiern collected from D.R. Congo was analyzed by GC and GC/MS. Thirty three compounds representing 97.34% of the oil were identified. The major components were -eudesmol (49.1%), 10-epi--eudesmol (11.30%), -humulene (8.9%), hedycaryol (8.9%), and elemol (5.2%). The antibacterial activity of the essential oil and its dominant constituent was evaluated against four bacteria strains (Staphyloccocus aureus ATCC 25952, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 9027, Proteus vulgaris ATCC 4635) using the micro-dilution method. The results indicate that the two samples are active against all tested microorganisms. The oil has a moderate activity against Staphylococcus aureus and Pseudomonas aeruginosa with a minimum inhibitory concentration (MIC) value of 125 μg/mL. The isolated major constituent (-eudesmol) exhibits the highest activity against Staphylococcus aureus and Pseudomonas aeruginosa with ...

Department of Chemistry, Faculty of Sciences, University of Kinshasa, P.O Box 190, Kinshasa XI, D... more Department of Chemistry, Faculty of Sciences, University of Kinshasa, P.O Box 190, Kinshasa XI, Democratic Republic of the Congo. Department of Biology, Faculty of Sciences, University of Kinshasa, P.O Box 190, Kinshasa XI, Democratic Republic of the Congo. Department of Basic Sciences, Faculty of Medicine, University of Gbado-Lite, P.O Box 111, Gbado-Lite, Democratic Republic of the Congo. Faculty of Pharmaceutical Sciences, University of Kinshasa, Democratic Republic of Congo.

International Journal of Herbal Medicine, 2016

Hypoglycemic and antidiabetic activities and acute toxicity of extracts from leaves of Solanum ni... more Hypoglycemic and antidiabetic activities and acute toxicity of extracts from leaves of Solanum nigrum L., an edible plant used in the Eastern part of the Democratic Republic of the Congo against diabetes, were evaluated on guinea-pigs. The hypoglycemic activity was evaluated by glucose tolerance test by loading animal with glucose and measuring blood concentration at various times. For inducing experimental diabetes mellitus, Alloxan monohydrate was used in normal saline by subcutaneous administration and glibenclamide was used as standard drug. Acute toxicity was evaluated by recording mortality rate, changes on blood biomarkers and damage caused to vital organs. Obtained results showed a significant decrease (P˂0.05) in blood glucose levels in guinea-pigs treated with the aqueous extract of S. nigrum both in hypoglycemic and antidiabetic tests. The decrease in the group treated with S. nigrum aqueous extract was sometimes more pronounced than that of the positive control group, ju...

International Journal of Innovation and Applied Studies, 2016

The use of great apes Pharmacopoeia or zoo-pharmacognosy is a very promising strategy for the tre... more The use of great apes Pharmacopoeia or zoo-pharmacognosy is a very promising strategy for the treatment of human diseases due to the phylogenetic proximity of humans and non-human primates (NHP) as Bonobos. In total, more than 40 direct fields' observations of bonobos have been performed for five months. A high number of direct observations were recorded in August followed by September while there was less observations in June. The average size and composition of the group for August is estimated at 19 individuals with 8 males, 9 females and 2 young whereas the size recorded in September was of 12 individuals including 5 males, 6 females and 1 young. Physicochemical and blood-biochemical analysis results performed on bonobos urine within their natural habitat shown the presence of bilirubin in some urine specimens (a formation of an azo derivative with a diazonium salt). The presence of a lot of leukocytes in urine is an indication of Plasmodium infection since we are in Malaria...

Journal of Pharmacognosy and Phytochemistry, 2016

The aim of the present study was to evaluate the Chemical composition and bioactivity of Canarium... more The aim of the present study was to evaluate the Chemical composition and bioactivity of Canarium schweinfurthii stem bark extracts against Sickle cell disease and associated bacteria. The antisickling and antibacterial activities were carried out using Emmel and micro-dilution methods respectively. The results revealed that the stem bark of Canarium schweinfurthii contains various secondary metabolites such as the anthocyanins, flavonoids, tannins, quinones, saponins, alkaloids, steroids, terpenoids and leuco-anthocyanins. The n-hexane (non-polar solvent) extract displayed poor yield than the extracts obtained in the polar solvents which have a high yield. Flavonoids were most concentrated in the methanol solvent whereas the anthocyanins were concentrated in acidified methanol while the tannins were concentrated in n-hexane (condensed tannins). The extraction yield of organic/triterpenoic acids (betulinic acid rich extract) was 0.75%. All tested extracts displayed antisickling acti...

All over Africa, traditional healers use medicinal plants to prepare medicines to treat a wide ra... more All over Africa, traditional healers use medicinal plants to prepare medicines to treat a wide range of illnesses. One of these illnesses is sickle cell anaemia or drepanocytosis or sicklemia. This disease is particularly common among sub-Saharan Africans with a clear predominance in equatorial Africa. However, it also exists in North Africa, Greece, Turkey, Saudi Arabia and India. An estimated 50 million people are affected worldwide. A literature review on sickle cell anaemia revealed that a number of plants have anti-drepanocytosic activity. The availability and frequency of ethnobotanic use of plants were taken into account when selecting the plants investigated in this study. Cajanus cajan, Callistemon viminalis, Melaleuca bracteata var. Revolution Gold and Syzygium guineense (from DRC and South Africa) were selected for study. The selected plants were subjected to modern phytochemical analysis. A total of 8 compounds were isolated from the plants’ extracts and their structures...

Tropical Plant Research, 2016

An ethno-botanical survey was conducted among traditional healers and medicinal plant vendors in ... more An ethno-botanical survey was conducted among traditional healers and medicinal plant vendors in Kinshasa city (DR Congo) in order to identify plant species used in traditional medicine to treat common diseases, with the aim of documenting, preserving, and sustaining this valuable traditional knowledge. Surveys were conducted from February to April 2014 among 50 medicinal plant vendors, in five markets (Limete, Makala, Matete, Mont-Ngafula, and Ngaba). The education level of the majority of informants was secondary school. The age of the informants ranged between 20 and 68 years. Cited plant species were collected and identified at the herbarium of the Faculty of Science, University of Kinshasa. Their ecological status was also determined. The 50 informants used 32 plant species (belonging to 22 families and 30 genera) in traditional medicine in Kinshasa. Their herbal remedies were administered as aqueous decoctions against 38 different diseases. It was found that ligneous, savanna,...

ABSTRACT Twenty-two extracts from seven Congolese medicinal plants were phytochemically screened ... more ABSTRACT Twenty-two extracts from seven Congolese medicinal plants were phytochemically screened and evaluated for antisickling activities. Ethanol extract of the leaves of Jatropha curcas L., the aqueous extracts of the leaves of Cajanus cajan L. Melsp, Alchornea cordifolia Mull Arg and the aqueous extract of the roots of Hymenocardia acida Tul. exhibited attractive activities. Anthocyanins crude extract of the leaves of Jatropha curcas L. showed a significant in vitro normalization of sickle cell erythrocytes at 87%. However, this percentage of normalization decreases when the extract is submitted to the UV radiation and the heat.