Ute Siesenop - Academia.edu (original) (raw)
Papers by Ute Siesenop
Mycoses, May 1, 1995
Over a period of 28 days, 10 Trichophyton mentagrophytes strains were examined for their ability ... more Over a period of 28 days, 10 Trichophyton mentagrophytes strains were examined for their ability to secrete keratinolytic enzymes. Production of enzymes was stimulated by various keratins used as substrates. Duration and intensity of keratinase secretion were strongly influenced by the keratinous substrate. Duration and intensity of the enzyme production also differed among the 10 dermatophyte strains. Five different enzymes were isolated with molecular weights ranging from 28 kDa to 65 kDa. The different enzymes might be produced by different varieties of the species Trichophyton mentagrophytes.
Virology Journal
Bovine adenovirus 7 (BAdV-7) is an unclassified member of the genus Atadenovirus with a worldwide... more Bovine adenovirus 7 (BAdV-7) is an unclassified member of the genus Atadenovirus with a worldwide distribution and has been reported to induce clinical disease of varying severity in infected cattle, ranging from asymptomatic infections to severe enteric or respiratory disease. In this study, we used next-generation sequencing to obtain the first complete genome sequence of a European strain of BadV-7, from pooled spleen and liver tissue obtained from a deceased newborn Limousin calf. Histopathological analysis and electron microscopy showing systemic lesions in multiple organs with intranuclear amphophilic inclusions observed in endothelial cells in multiple peripheral tissues. Virus isolation was readily achieved from tissue homogenate using bovine esophagus cells (KOP-R), a strategy which should facilitate future in vitro or in vivo BAdV-7 studies. Phylogenetic analysis of available genome sequences of BAdV-7 showed that the newly identified strain groups most closely with a rece...
Tagung der DVG-Fachgruppe Krankheiten kleiner Wiederkäuer: Greifswald - Insel Riems, 04. bis 06. September 2019, 2019
Kleintierpraxis, May 1, 2008
Gegenstand und Ziel: Evaluierung der hygienischen Beschaffenheit von Futtermitteln fur Kleinsauge... more Gegenstand und Ziel: Evaluierung der hygienischen Beschaffenheit von Futtermitteln fur Kleinsauger wie Kaninchen, Meerschweinchen oder Chinchilla in der Heimtierhaltung. Material und Methoden: Beim Untersuchungsgut handelte es sich um 356 Futtermittel, die zwischen Januar 2000 und April 2011 mit einem „verwertbaren“ Vorbericht an das Institut eingesandt worden waren. Die Proben unterlagen einer sensorischen Prufung sowie einer mikrobiologischen Untersuchung. Bei den kulturell nachgewiesenen Bakterien und Pilzen erfolgte eine Differenzierung zwischen produkttypischen und verderbanzeigenden Keimen. Zur Beurteilung der mikrobiologischen Befunde dienten aktuelle Orientierungswerte des Arbeitskreises „Futtermittelmikrobiologie der Fachgruppe VI der VDLUFA“ fur Heu, Stroh, Einzel- und Mischfuttermittel. Ergebnisse: In der sensorischen Prufung lieferten insbesondere die Parameter “Griff” (Anhaltspunkt fur den Feuchtegehalt) und “Geruch” (schimmelige oder hefige Nuancen) wichtige Hinweise. ...
62. Jahrestagung der Fachgruppe Pathologie der Deutschen Veterinärmedizinischen Gesellschaft, 2019
Berliner und Münchener tierärztliche Wochenschrift, 1998
In 1993 and 1994 a highly increased occurrence of equine dermatophilosis was observed, and a stud... more In 1993 and 1994 a highly increased occurrence of equine dermatophilosis was observed, and a study was initiated to determine phenotypic heterogeneity among 120 clinical isolates using biochemistry, antibiotic resistance profiles, membrane protein profiles and Western blotting. The biochemical examinations contained 1% equine serum in medium. Moreover, the API ZYM-test from bioMérieux was used. The biochemical reactions were suited to identify Dermatophilus congolensis but did not allow a differentiation among the various isolates. Antibiotic resistance in one or more isolates was observed against polymyxin B, enrofloxacin, oxacillin, neomycin and trimethoprim-sulfamethoxazol. All isolates were sensitive penicillin G, ampicillin, streptomycin, gentamicin, lincomycin, erythromycin, tetracycline, oxytetracycline, bacitracin and ceftiofur. The evaluation of silver-stained and immuno-stained membrane protein profiles showed minor differences among several isolates. In total, all isolate...
DTW. Deutsche tierärztliche Wochenschrift, 1997
In 2 laying hen flocks (housed in aviary systems) depressed performance, increased mortality and ... more In 2 laying hen flocks (housed in aviary systems) depressed performance, increased mortality and cannibalism were observed. Specific infectious diseases were excluded. The hygienic quality of the diet, however, based on self produced feed ingredients, was impaired. High concentrations of bacteria, moulds, and yeasts were found in the complete diet and also in the ingredients. These problems were eliminated by providing the birds with good quality feed ingredients. In a diagnostic feeding trial with a change in housing conditions hens showed a good performance. The poor feed quality, as demonstrated in this report, is regarded to be one of several factors, which have contributed to the problems observed in these flocks. Other negative environmental factors such as housing have to be eliminated.
Tierärztliche Praxis, 1997
A dermatomycosis of swine caused by Trichophyton verrucosum is reported. Trichophyton verrucosum ... more A dermatomycosis of swine caused by Trichophyton verrucosum is reported. Trichophyton verrucosum could be identified in skin scrapings of pigs and contacting calves by fluorescence microscopy and culture. The mode of transmission and the rare isolation of Trichophyton verrucosum from pigs are discussed.
Mycoses, 1995
Over a period of 28 days, 10 Trichophyton mentagrophytes strains were examined for their ability ... more Over a period of 28 days, 10 Trichophyton mentagrophytes strains were examined for their ability to secrete keratinolytic enzymes. Production of enzymes was stimulated by various keratins used as substrates. Duration and intensity of keratinase secretion were strongly influenced by the keratinous substrate. Duration and intensity of the enzyme production also differed among the 10 dermatophyte strains. Five different enzymes were isolated with molecular weights ranging from 28 kDa to 65 kDa. The different enzymes might be produced by different varieties of the species Trichophyton mentagrophytes.
Mycoses, 1999
A total of 220 isolates of the genus Malassezia obtained from affected skin of animals and humans... more A total of 220 isolates of the genus Malassezia obtained from affected skin of animals and humans were included in this study. The isolates were examined for their belonging to the known species Malassezia pachydermatis, Malassezia sympodialis, Malassezia furfur, Malassezia obtusa, Malassezia restricta, Malassezia globosa and Malassezia slooffiae by means of phenotypic and biochemical characteristics as well as their ability to assimilate polyethylen(20)‐sorbitan‐esters (Tween 20, 40, 60, 80). The electrophoretic karyotypes of all isolates were studied by pulsed‐field gel electrophoresis. Of the 220 Malassezia isolates examined, 217 were identified as M. pachydermatis and three were classified as M. sympodialis. Moreover, the proof of limited lipid‐dependence of M. pachydermatis indicated that the commonly used criterion ‘lipid‐independence’ is not sufficient to differentiate this species from the other known Malassezia species.
PLOS ONE, 2022
Mycoplasma hyorhinis is an emerging swine pathogen bacterium causing polyserositis and polyarthri... more Mycoplasma hyorhinis is an emerging swine pathogen bacterium causing polyserositis and polyarthritis in weaners and finishers. The pathogen is distributed worldwide , generating significant economic losses. No commercially available vaccine is available in Europe. Therefore, besides improving the housing conditions for prevention, antimicrobial therapy of the diseased animals is the only option to control the infection. Our aim was to determine the minimal inhibitory concentrations (MIC) of ten antimicrobials potentially used against M. hyorhinis infection. The antibiotic susceptibility of 76 M. hyorhinis isolates from Belgium, Germany, Hungary, Italy and Poland collected between 2019 and 2021 was determined by broth micro-dilution method and mismatch amplification mutation assay (MAMA). Low concentrations of tiamulin (MIC 90 0.312 μg/ml), doxycycline (MIC 90 0.078 μg/ml), oxytetracycline (MIC 90 0.25 μg/ml), florfenicol (MIC 90 2 μg/ml) and moderate concentrations of enrofloxacin (MIC 90 1.25 μg/ml) inhibited the growth of the isolates. For the tested macrolides and lincomycin, a bimodal MIC pattern was observed (MIC 90 >64 μg/ml for lincomycin, tulathromycin, tylosin and tilmicosin and 5 μg/ml for tylvalosin). The results of the MAMA assay were in line with the conventional method with three exceptions. Based on our statistical analyses, significant differences in MIC values of tiamulin and doxycycline were observed between certain countries. Our results show various levels of antimicrobial susceptibility among M. hyorhinis isolates to the tested antibiotics. The data underline the importance of susceptibility monitoring on pan-European level and provides essential information for proper antibiotic choice in therapy.
Mycoses (Berlin), 1994
We determined the intensity of growth, the ability to form spores and virulence in guinea pigs of... more We determined the intensity of growth, the ability to form spores and virulence in guinea pigs of the vaccine strain of the Czech live vaccine Bioveta. Comparative investigations were carried out with the vaccine strain of the Russian live vaccine LTF 130 and Trichophyton verrucosum field strains. Growth of the vaccine strains was faster than growth of the field strains on solid media and in broth. Later the vaccine strains formed many microconidia. In experimentally infected guinea pigs the virulence of the vaccine strain Bioveta was highly dependent on the infectious dose. At 10(1) CFU ml-1 (according to the number of CFUs in the Russian live vaccine) the vaccine strain Bioveta was nearly avirulent. Resuspended according to the manufacturer's instructions (at least 10(10) CFU ml-1) virulence of the vaccine strain Bioveta could be observed.
Veterinary microbiology, 2017
Listeriosis is a zoonotic infection with the gram positive, facultative intracellular bacterium L... more Listeriosis is a zoonotic infection with the gram positive, facultative intracellular bacterium Listeria (L.) monocytogenes. Infections mainly occur in ruminants, but also in other species, including humans. Case fatality rate usually is high. The incidence of listeriosis in captive non-human primates is very low. We report the first spontaneous, fatal, and likely food-born outbreak of listeriosis in a population of captive grey mouse lemurs (Microcebus murinus). Conspicuously, none of the closely related Goodman's mouse lemurs (Microcebus lehilahytsara) in the same facility were affected.
Mycoses, May 1, 1995
Over a period of 28 days, 10 Trichophyton mentagrophytes strains were examined for their ability ... more Over a period of 28 days, 10 Trichophyton mentagrophytes strains were examined for their ability to secrete keratinolytic enzymes. Production of enzymes was stimulated by various keratins used as substrates. Duration and intensity of keratinase secretion were strongly influenced by the keratinous substrate. Duration and intensity of the enzyme production also differed among the 10 dermatophyte strains. Five different enzymes were isolated with molecular weights ranging from 28 kDa to 65 kDa. The different enzymes might be produced by different varieties of the species Trichophyton mentagrophytes.
Virology Journal
Bovine adenovirus 7 (BAdV-7) is an unclassified member of the genus Atadenovirus with a worldwide... more Bovine adenovirus 7 (BAdV-7) is an unclassified member of the genus Atadenovirus with a worldwide distribution and has been reported to induce clinical disease of varying severity in infected cattle, ranging from asymptomatic infections to severe enteric or respiratory disease. In this study, we used next-generation sequencing to obtain the first complete genome sequence of a European strain of BadV-7, from pooled spleen and liver tissue obtained from a deceased newborn Limousin calf. Histopathological analysis and electron microscopy showing systemic lesions in multiple organs with intranuclear amphophilic inclusions observed in endothelial cells in multiple peripheral tissues. Virus isolation was readily achieved from tissue homogenate using bovine esophagus cells (KOP-R), a strategy which should facilitate future in vitro or in vivo BAdV-7 studies. Phylogenetic analysis of available genome sequences of BAdV-7 showed that the newly identified strain groups most closely with a rece...
Tagung der DVG-Fachgruppe Krankheiten kleiner Wiederkäuer: Greifswald - Insel Riems, 04. bis 06. September 2019, 2019
Kleintierpraxis, May 1, 2008
Gegenstand und Ziel: Evaluierung der hygienischen Beschaffenheit von Futtermitteln fur Kleinsauge... more Gegenstand und Ziel: Evaluierung der hygienischen Beschaffenheit von Futtermitteln fur Kleinsauger wie Kaninchen, Meerschweinchen oder Chinchilla in der Heimtierhaltung. Material und Methoden: Beim Untersuchungsgut handelte es sich um 356 Futtermittel, die zwischen Januar 2000 und April 2011 mit einem „verwertbaren“ Vorbericht an das Institut eingesandt worden waren. Die Proben unterlagen einer sensorischen Prufung sowie einer mikrobiologischen Untersuchung. Bei den kulturell nachgewiesenen Bakterien und Pilzen erfolgte eine Differenzierung zwischen produkttypischen und verderbanzeigenden Keimen. Zur Beurteilung der mikrobiologischen Befunde dienten aktuelle Orientierungswerte des Arbeitskreises „Futtermittelmikrobiologie der Fachgruppe VI der VDLUFA“ fur Heu, Stroh, Einzel- und Mischfuttermittel. Ergebnisse: In der sensorischen Prufung lieferten insbesondere die Parameter “Griff” (Anhaltspunkt fur den Feuchtegehalt) und “Geruch” (schimmelige oder hefige Nuancen) wichtige Hinweise. ...
62. Jahrestagung der Fachgruppe Pathologie der Deutschen Veterinärmedizinischen Gesellschaft, 2019
Berliner und Münchener tierärztliche Wochenschrift, 1998
In 1993 and 1994 a highly increased occurrence of equine dermatophilosis was observed, and a stud... more In 1993 and 1994 a highly increased occurrence of equine dermatophilosis was observed, and a study was initiated to determine phenotypic heterogeneity among 120 clinical isolates using biochemistry, antibiotic resistance profiles, membrane protein profiles and Western blotting. The biochemical examinations contained 1% equine serum in medium. Moreover, the API ZYM-test from bioMérieux was used. The biochemical reactions were suited to identify Dermatophilus congolensis but did not allow a differentiation among the various isolates. Antibiotic resistance in one or more isolates was observed against polymyxin B, enrofloxacin, oxacillin, neomycin and trimethoprim-sulfamethoxazol. All isolates were sensitive penicillin G, ampicillin, streptomycin, gentamicin, lincomycin, erythromycin, tetracycline, oxytetracycline, bacitracin and ceftiofur. The evaluation of silver-stained and immuno-stained membrane protein profiles showed minor differences among several isolates. In total, all isolate...
DTW. Deutsche tierärztliche Wochenschrift, 1997
In 2 laying hen flocks (housed in aviary systems) depressed performance, increased mortality and ... more In 2 laying hen flocks (housed in aviary systems) depressed performance, increased mortality and cannibalism were observed. Specific infectious diseases were excluded. The hygienic quality of the diet, however, based on self produced feed ingredients, was impaired. High concentrations of bacteria, moulds, and yeasts were found in the complete diet and also in the ingredients. These problems were eliminated by providing the birds with good quality feed ingredients. In a diagnostic feeding trial with a change in housing conditions hens showed a good performance. The poor feed quality, as demonstrated in this report, is regarded to be one of several factors, which have contributed to the problems observed in these flocks. Other negative environmental factors such as housing have to be eliminated.
Tierärztliche Praxis, 1997
A dermatomycosis of swine caused by Trichophyton verrucosum is reported. Trichophyton verrucosum ... more A dermatomycosis of swine caused by Trichophyton verrucosum is reported. Trichophyton verrucosum could be identified in skin scrapings of pigs and contacting calves by fluorescence microscopy and culture. The mode of transmission and the rare isolation of Trichophyton verrucosum from pigs are discussed.
Mycoses, 1995
Over a period of 28 days, 10 Trichophyton mentagrophytes strains were examined for their ability ... more Over a period of 28 days, 10 Trichophyton mentagrophytes strains were examined for their ability to secrete keratinolytic enzymes. Production of enzymes was stimulated by various keratins used as substrates. Duration and intensity of keratinase secretion were strongly influenced by the keratinous substrate. Duration and intensity of the enzyme production also differed among the 10 dermatophyte strains. Five different enzymes were isolated with molecular weights ranging from 28 kDa to 65 kDa. The different enzymes might be produced by different varieties of the species Trichophyton mentagrophytes.
Mycoses, 1999
A total of 220 isolates of the genus Malassezia obtained from affected skin of animals and humans... more A total of 220 isolates of the genus Malassezia obtained from affected skin of animals and humans were included in this study. The isolates were examined for their belonging to the known species Malassezia pachydermatis, Malassezia sympodialis, Malassezia furfur, Malassezia obtusa, Malassezia restricta, Malassezia globosa and Malassezia slooffiae by means of phenotypic and biochemical characteristics as well as their ability to assimilate polyethylen(20)‐sorbitan‐esters (Tween 20, 40, 60, 80). The electrophoretic karyotypes of all isolates were studied by pulsed‐field gel electrophoresis. Of the 220 Malassezia isolates examined, 217 were identified as M. pachydermatis and three were classified as M. sympodialis. Moreover, the proof of limited lipid‐dependence of M. pachydermatis indicated that the commonly used criterion ‘lipid‐independence’ is not sufficient to differentiate this species from the other known Malassezia species.
PLOS ONE, 2022
Mycoplasma hyorhinis is an emerging swine pathogen bacterium causing polyserositis and polyarthri... more Mycoplasma hyorhinis is an emerging swine pathogen bacterium causing polyserositis and polyarthritis in weaners and finishers. The pathogen is distributed worldwide , generating significant economic losses. No commercially available vaccine is available in Europe. Therefore, besides improving the housing conditions for prevention, antimicrobial therapy of the diseased animals is the only option to control the infection. Our aim was to determine the minimal inhibitory concentrations (MIC) of ten antimicrobials potentially used against M. hyorhinis infection. The antibiotic susceptibility of 76 M. hyorhinis isolates from Belgium, Germany, Hungary, Italy and Poland collected between 2019 and 2021 was determined by broth micro-dilution method and mismatch amplification mutation assay (MAMA). Low concentrations of tiamulin (MIC 90 0.312 μg/ml), doxycycline (MIC 90 0.078 μg/ml), oxytetracycline (MIC 90 0.25 μg/ml), florfenicol (MIC 90 2 μg/ml) and moderate concentrations of enrofloxacin (MIC 90 1.25 μg/ml) inhibited the growth of the isolates. For the tested macrolides and lincomycin, a bimodal MIC pattern was observed (MIC 90 >64 μg/ml for lincomycin, tulathromycin, tylosin and tilmicosin and 5 μg/ml for tylvalosin). The results of the MAMA assay were in line with the conventional method with three exceptions. Based on our statistical analyses, significant differences in MIC values of tiamulin and doxycycline were observed between certain countries. Our results show various levels of antimicrobial susceptibility among M. hyorhinis isolates to the tested antibiotics. The data underline the importance of susceptibility monitoring on pan-European level and provides essential information for proper antibiotic choice in therapy.
Mycoses (Berlin), 1994
We determined the intensity of growth, the ability to form spores and virulence in guinea pigs of... more We determined the intensity of growth, the ability to form spores and virulence in guinea pigs of the vaccine strain of the Czech live vaccine Bioveta. Comparative investigations were carried out with the vaccine strain of the Russian live vaccine LTF 130 and Trichophyton verrucosum field strains. Growth of the vaccine strains was faster than growth of the field strains on solid media and in broth. Later the vaccine strains formed many microconidia. In experimentally infected guinea pigs the virulence of the vaccine strain Bioveta was highly dependent on the infectious dose. At 10(1) CFU ml-1 (according to the number of CFUs in the Russian live vaccine) the vaccine strain Bioveta was nearly avirulent. Resuspended according to the manufacturer's instructions (at least 10(10) CFU ml-1) virulence of the vaccine strain Bioveta could be observed.
Veterinary microbiology, 2017
Listeriosis is a zoonotic infection with the gram positive, facultative intracellular bacterium L... more Listeriosis is a zoonotic infection with the gram positive, facultative intracellular bacterium Listeria (L.) monocytogenes. Infections mainly occur in ruminants, but also in other species, including humans. Case fatality rate usually is high. The incidence of listeriosis in captive non-human primates is very low. We report the first spontaneous, fatal, and likely food-born outbreak of listeriosis in a population of captive grey mouse lemurs (Microcebus murinus). Conspicuously, none of the closely related Goodman's mouse lemurs (Microcebus lehilahytsara) in the same facility were affected.