Volker von Baehr - Academia.edu (original) (raw)

Papers by Volker von Baehr

Research paper thumbnail of Aseptic-avascular osteonecrosis: local "silent inflammation" in the jawbone and RANTES/CCL5 overexpression

Clinical, cosmetic and investigational dentistry, 2017

Of the definitions listed in the International Statistical Classification of Diseases and Related... more Of the definitions listed in the International Statistical Classification of Diseases and Related Health Problems, tenth revision (ICD-10), two disease descriptions can be found together: "idiopathic aseptic bone necrosis" and "avascular bone necrosis." The relevant literature on both the conditions abbreviates both as "aseptic ischemic osteonecrosis in the jawbone" (AIOJ). To shed light on the clinical details of this condition, osteolytic jawbone samples of 24 patients with different systemic immunological diseases were examined using four steps: presurgical dental X-ray, postsurgical histology, polymerase chain reaction DNA analysis (PCR DNA) of bacteria, and RANTES/CCL5 (R/C) expression. These four steps showed that neither X-ray nor histology delivered unambiguous results with respect to inflammatory processes; furthermore, the PCR results did not show evidence of any microbial load within the jaw samples. However, there is a striking, coherent ove...

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Research paper thumbnail of Onkogenes Schlüsselsignal RANTES/CCL5 – „Cytokine Cross Talk“ des Tumors und „Silent Inflammation“ des Kieferknochens

Deutsche Zeitschrift für Onkologie

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Research paper thumbnail of Sorafenib-Induced Severe Acute Hepatitis in a Stable Liver Transplant Recipient

Transplantation, Jul 15, 2010

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Research paper thumbnail of Abstract-Le-vBaehr-PeriphNeuropathPain

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Research paper thumbnail of Impaired antigen presentation by human monocytes during endotoxin tolerance

Blood, 2000

Endotoxin tolerance (ET) has been described as a temporary alteration in the lipopolysaccharide (... more Endotoxin tolerance (ET) has been described as a temporary alteration in the lipopolysaccharide (LPS) response of monocytic cells after an initial LPS exposure with respect to the production of soluble immunomodulators. Apart from the LPS response, monocytic cells play an important role in initiation of the specific immune response as antigen-presenting cells. This study investigated the capacity of human blood monocytes to induce T-cell stimulation in ET. First, the expression of monocyte surface molecules, important for T-cell interaction, was analyzed by flow cytometry. In vitro priming of peripheral blood mononuclear cells with LPS clearly down-regulates major histocompatibility complex class II molecules and the costimulatory molecule CD86. Both changes were dependent on the endogenous interleukin (IL)-10 and less so on the transforming growth factor-beta. In contrast, other accessory molecules on monocytes were only marginally down-regulated (CD58), were not significantly chan...

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Research paper thumbnail of The lymphocyte transformation test for borrelia detects active lyme borreliosis and verifies effective antibiotic treatment

The open neurology journal, 2012

Borrelia-specific antibodies are not detectable until several weeks after infection and even if t... more Borrelia-specific antibodies are not detectable until several weeks after infection and even if they are present, they are no proof of an active infection. Since the sensitivity of culture and PCR for the diagnosis or exclusion of borreliosis is too low, a method is required that detects an active Borrelia infection as early as possible. For this purpose, a lymphocyte transformation test (LTT) using lysate antigens of Borrelia burgdorferi sensu stricto, Borrelia afzelii and Borrelia garinii and recombinant OspC was developed and validated through investigations of seronegative and seropositive healthy individuals as well as of seropositive patients with clinically manifested borreliosis. The sensitivity of the LTT in clinical borreliosis before antibiotic treatment was determined as 89,4% while the specificity was 98,7%. In 1480 patients with clinically suspected borreliosis, results from serology and LTT were comparable in 79.8% of cases. 18% were serologically positive and LTT-neg...

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Research paper thumbnail of Allergoid-specific T-cell reaction as a measure of the immunological response to specific immunotherapy (SIT) with a Th1-adjuvanted allergy vaccine

Journal of investigational allergology & clinical immunology, 2005

Specific immunotherapy (SIT) is believed to modulate CD4+ T-helper cells. In order to improve saf... more Specific immunotherapy (SIT) is believed to modulate CD4+ T-helper cells. In order to improve safety, SIT vaccines are often formulated with allergoids (chemically modified allergens). Interaction between T-cells and allergoids is necessary to influence cellular cytokine expression. There have been few reports on identification the early cellular effects of SIT. Patients allergic to grass and/or mugwort pollen (n= 21) were treated with a 4-shot allergy vaccine (Pollinex Quattro) containing appropriate allergoids (grass/rye and/or mugwort) adsorbed to L-tyrosine plus a Th1 adjuvant, monophosphoryl lipid A (MPL). Fourteen grass-allergic patients served as untreated controls. Using the peripheral blood mononuclear cells of these patients, an optimized lymphocyte transformation test (LTT) was employed to monitor the in vitro proliferative response of T-cells to an allergoid challenge (solubilised Pollinex Quattro) before the first and last injection and then 2 and 20 weeks after the fin...

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Research paper thumbnail of Untersuchungen zur diagnostischen Wertigkeit des Lymphozytentransformationstestes bei Patienten mit Borreliose / Evaluation of the diagnostic significance of the lymphocyte proliferation test in patients with Lyme borreliosis

LaboratoriumsMedizin, 2007

... Volker von Baehr1, Christa Liebenthal2, Brita Gaida1, Frank-Peter Schmidt1, Ru¨ diger von Bae... more ... Volker von Baehr1, Christa Liebenthal2, Brita Gaida1, Frank-Peter Schmidt1, Ru¨ diger von Baehr1,* und Hans-Dieter Volk2 1 Institut fu¨ r Medizinische Diagnostik, Abteilung Immunologie, Berlin, Deutschland 2 Humboldt-Universita¨ t-Berlin, Institut fu¨ r medizinische ...

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Research paper thumbnail of Sorafenib-Induced Severe Acute Hepatitis in a Stable Liver Transplant Recipient

Transplantation, 2010

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Research paper thumbnail of Deficient EBV-Specific B- and T-Cell Response in Patients with Chronic Fatigue Syndrome

PLoS ONE, 2014

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Research paper thumbnail of Comparison of Monocyte Functions after LPS- or IL-10-Induced Reorientation: Importance in Clinical Immunoparalysis

Pathobiology, 1999

Immunoparalysis is an acquired immunodeficiency which may occur in patients after major surgery, ... more Immunoparalysis is an acquired immunodeficiency which may occur in patients after major surgery, burns, polytrauma and sepsis. It is associated with a modified state of monocytes marked by their altered capacity to induce antigen-specific T cell stimulation and to release various cytokines. However, the pathogenesis of immunoparalysis may differ in various patient groups. It can develop in patients after systemic hyperinflammation induced by gastrointestinal translocation of endotoxin (lipopolysaccharide, LPS) or sepsis, as well as in patients without preceding systemic inflammation but primary anti-inflammation, for instance induced by sympathetic activation. To further elucidate the syndrome, we compared endotoxin tolerance as a model of immunoparalysis after systemic hyperinflammation versus interleukin-10 (IL-10) treatment as a model of primarily anti-inflammation-induced immunoparalysis. In vitro priming of peripheral blood mononuclear cells with either LPS or IL-10 for 24 h led to a strongly or moderately diminished LPS-induced tumor necrosis factor-alpha (TNF-alpha) production, compared to unprimed controls, respectively. Furthermore, LPS-induced reduction of TNF-alpha production capacity persisted over the following days whereas IL-10-primed monocytes rapidly recovered. Similarly, in contrast to persistently diminished MHC class II expression in LPS-treated monocytes, IL-10 only transiently downregulated these molecules. Consequently, in contrast to IL-10-primed monocytes, LPS-primed monocytes were greatly impaired in their capacity to induce antigen-specific T cell proliferation and IFN-gamma production. These data indicate that LPS priming provokes a more profound modulation of monocyte function than IL-10 priming, raising the question of possible variations in the clinical course of immunoparalysis, dependent on its pathogenesis.

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Research paper thumbnail of Transfer of Autologous Gene-modified T Cells in HIV-infected Patients with Advanced Immunodeficiency and Drug-resistant Virus

Molecular Therapy, 2007

Drug toxicity and viral resistance limit the long-term efficacy of antiviral drug treatment for h... more Drug toxicity and viral resistance limit the long-term efficacy of antiviral drug treatment for human immunodeficiency virus (HIV) infection. Thus, alternative therapies need to be explored. We tested the infusion of T lymphocytes transduced with a retroviral vector (M87o) that expresses an HIV entry-inhibitory peptide (maC46). Gene-modified autologous T cells were infused into ten HIV-infected patients with advanced disease and multidrug-resistant virus during anti-retroviral combination therapy. T-cell infusions were tolerated well, with no severe side effects. A significant increase of CD4 counts was observed after infusion. At the end of the 1-year follow-up, the CD4 counts of all patients were still around or above baseline. Gene-modified cells could be detected in peripheral blood, lymph nodes, and bone marrow throughout the 1-year follow-up, and marking levels correlated with the cell dose. No significant changes of viral load were observed during the first 4 months. Four of the seven patients who changed their antiviral drug regimen thereafter responded with a significant decline in plasma viral load. In conclusion, the transfer of gene-modified cells was safe, led to sustained levels of gene marking, and may improve immune competence in HIV-infected patients with advanced disease and multidrug-resistant virus.

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Research paper thumbnail of Heterozygous Arg753Gln polymorphism of human TLR-2 impairs immune activation by Borrelia burgdorferi and protects from late stage Lyme disease

The Journal of Immunology, 2006

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Research paper thumbnail of Improving the in vitro antigen specific T cell proliferation assay: the use of interferon-alpha to elicit antigen specific stimulation and decrease bystander proliferation

Journal of Immunological Methods, 2001

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Research paper thumbnail of Impaired immune function in patients with alcoholic liver cirrhosis(ALC) due to active cytomegalovirus (CMV) infection

Journal of Hepatology, 2000

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Research paper thumbnail of Correlation between energy expenditure, nutrient intake, malnutrition and activation of the inflammatory system in patients with hepatic cirrhosis

Journal of Hepatology, 2001

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Research paper thumbnail of Activation of immune functions in patients with liver cirrhosis (LC) by selective gut decontamination (SGD)

Journal of Hepatology, 2001

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Research paper thumbnail of Immunologic Effector Mechanisms of a Standardized Mistletoe Extract on the Function of Human Monocytes and Lymphocytes in vitro, ex vivo, and in vivo

Journal of Clinical Immunology, 2006

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Research paper thumbnail of Compound deletion of the rhoGAP C1 and V2 vasopressin receptor genes in a patient with nephrogenic diabetes insipidus

Human Mutation, 1999

The function of small GTPases is fine-tuned by a complex network of regulatory proteins such as G... more The function of small GTPases is fine-tuned by a complex network of regulatory proteins such as GTPase-activating proteins. The C1 gene at Xq28 encodes a protein assumed to function as a Rho GTPase-activating protein (rhoGAP). Characterization of the molecular defect causing X-linked nephrogenic diabetes insipidus (NDI) in a patient revealed a submicroscopic deletion of a 21.5-kb genomic fragment encompassing the entire arginine-vasopressin V2 receptor gene (AVPR2) and most of the C1 gene locus. In the absence of detailed information about the physiological relevance and specific functions of rhoGAP C1, a thorough clinical and laboratory investigation of the patient was performed. Besides clearly defined NDI symptoms caused by deletion of the AVPR2 gene, no major morphological abnormalities as determined by physical examination, radiography, ultrasound, and computed tomographic scan were detected. Extensive analysis of blood chemical, enzyme, and hormone values over a period of 16 years showed no deviations from normal ranges. On the basis of our observations, the rhoGAP C1 protein is not essential for normal development in the human. Because of a predominant expression pattern of the C1 gene in hematopoietic cells, we focused on immunologic and hematologic laboratory parameters of the affected boy and the mother who was found to be heterozygous. Differential white cell counts, including lymphocyte typing, determination of lymphokines, cytokines, and immunoglobulins, as well as numerous leukocyte function tests, showed no pathological findings. Therefore, we postulate that the loss of rhoGAP C1 function is most likely compensated by other members of the GAP family.

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Research paper thumbnail of Clinical manifestation of mannose-binding lectin deficiency in adults independent of concomitant immunodeficiency

Human Immunology, 2009

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Research paper thumbnail of Aseptic-avascular osteonecrosis: local "silent inflammation" in the jawbone and RANTES/CCL5 overexpression

Clinical, cosmetic and investigational dentistry, 2017

Of the definitions listed in the International Statistical Classification of Diseases and Related... more Of the definitions listed in the International Statistical Classification of Diseases and Related Health Problems, tenth revision (ICD-10), two disease descriptions can be found together: "idiopathic aseptic bone necrosis" and "avascular bone necrosis." The relevant literature on both the conditions abbreviates both as "aseptic ischemic osteonecrosis in the jawbone" (AIOJ). To shed light on the clinical details of this condition, osteolytic jawbone samples of 24 patients with different systemic immunological diseases were examined using four steps: presurgical dental X-ray, postsurgical histology, polymerase chain reaction DNA analysis (PCR DNA) of bacteria, and RANTES/CCL5 (R/C) expression. These four steps showed that neither X-ray nor histology delivered unambiguous results with respect to inflammatory processes; furthermore, the PCR results did not show evidence of any microbial load within the jaw samples. However, there is a striking, coherent ove...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Onkogenes Schlüsselsignal RANTES/CCL5 – „Cytokine Cross Talk“ des Tumors und „Silent Inflammation“ des Kieferknochens

Deutsche Zeitschrift für Onkologie

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Research paper thumbnail of Sorafenib-Induced Severe Acute Hepatitis in a Stable Liver Transplant Recipient

Transplantation, Jul 15, 2010

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Research paper thumbnail of Abstract-Le-vBaehr-PeriphNeuropathPain

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Impaired antigen presentation by human monocytes during endotoxin tolerance

Blood, 2000

Endotoxin tolerance (ET) has been described as a temporary alteration in the lipopolysaccharide (... more Endotoxin tolerance (ET) has been described as a temporary alteration in the lipopolysaccharide (LPS) response of monocytic cells after an initial LPS exposure with respect to the production of soluble immunomodulators. Apart from the LPS response, monocytic cells play an important role in initiation of the specific immune response as antigen-presenting cells. This study investigated the capacity of human blood monocytes to induce T-cell stimulation in ET. First, the expression of monocyte surface molecules, important for T-cell interaction, was analyzed by flow cytometry. In vitro priming of peripheral blood mononuclear cells with LPS clearly down-regulates major histocompatibility complex class II molecules and the costimulatory molecule CD86. Both changes were dependent on the endogenous interleukin (IL)-10 and less so on the transforming growth factor-beta. In contrast, other accessory molecules on monocytes were only marginally down-regulated (CD58), were not significantly chan...

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Research paper thumbnail of The lymphocyte transformation test for borrelia detects active lyme borreliosis and verifies effective antibiotic treatment

The open neurology journal, 2012

Borrelia-specific antibodies are not detectable until several weeks after infection and even if t... more Borrelia-specific antibodies are not detectable until several weeks after infection and even if they are present, they are no proof of an active infection. Since the sensitivity of culture and PCR for the diagnosis or exclusion of borreliosis is too low, a method is required that detects an active Borrelia infection as early as possible. For this purpose, a lymphocyte transformation test (LTT) using lysate antigens of Borrelia burgdorferi sensu stricto, Borrelia afzelii and Borrelia garinii and recombinant OspC was developed and validated through investigations of seronegative and seropositive healthy individuals as well as of seropositive patients with clinically manifested borreliosis. The sensitivity of the LTT in clinical borreliosis before antibiotic treatment was determined as 89,4% while the specificity was 98,7%. In 1480 patients with clinically suspected borreliosis, results from serology and LTT were comparable in 79.8% of cases. 18% were serologically positive and LTT-neg...

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Research paper thumbnail of Allergoid-specific T-cell reaction as a measure of the immunological response to specific immunotherapy (SIT) with a Th1-adjuvanted allergy vaccine

Journal of investigational allergology & clinical immunology, 2005

Specific immunotherapy (SIT) is believed to modulate CD4+ T-helper cells. In order to improve saf... more Specific immunotherapy (SIT) is believed to modulate CD4+ T-helper cells. In order to improve safety, SIT vaccines are often formulated with allergoids (chemically modified allergens). Interaction between T-cells and allergoids is necessary to influence cellular cytokine expression. There have been few reports on identification the early cellular effects of SIT. Patients allergic to grass and/or mugwort pollen (n= 21) were treated with a 4-shot allergy vaccine (Pollinex Quattro) containing appropriate allergoids (grass/rye and/or mugwort) adsorbed to L-tyrosine plus a Th1 adjuvant, monophosphoryl lipid A (MPL). Fourteen grass-allergic patients served as untreated controls. Using the peripheral blood mononuclear cells of these patients, an optimized lymphocyte transformation test (LTT) was employed to monitor the in vitro proliferative response of T-cells to an allergoid challenge (solubilised Pollinex Quattro) before the first and last injection and then 2 and 20 weeks after the fin...

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Research paper thumbnail of Untersuchungen zur diagnostischen Wertigkeit des Lymphozytentransformationstestes bei Patienten mit Borreliose / Evaluation of the diagnostic significance of the lymphocyte proliferation test in patients with Lyme borreliosis

LaboratoriumsMedizin, 2007

... Volker von Baehr1, Christa Liebenthal2, Brita Gaida1, Frank-Peter Schmidt1, Ru¨ diger von Bae... more ... Volker von Baehr1, Christa Liebenthal2, Brita Gaida1, Frank-Peter Schmidt1, Ru¨ diger von Baehr1,* und Hans-Dieter Volk2 1 Institut fu¨ r Medizinische Diagnostik, Abteilung Immunologie, Berlin, Deutschland 2 Humboldt-Universita¨ t-Berlin, Institut fu¨ r medizinische ...

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Research paper thumbnail of Sorafenib-Induced Severe Acute Hepatitis in a Stable Liver Transplant Recipient

Transplantation, 2010

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Deficient EBV-Specific B- and T-Cell Response in Patients with Chronic Fatigue Syndrome

PLoS ONE, 2014

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Comparison of Monocyte Functions after LPS- or IL-10-Induced Reorientation: Importance in Clinical Immunoparalysis

Pathobiology, 1999

Immunoparalysis is an acquired immunodeficiency which may occur in patients after major surgery, ... more Immunoparalysis is an acquired immunodeficiency which may occur in patients after major surgery, burns, polytrauma and sepsis. It is associated with a modified state of monocytes marked by their altered capacity to induce antigen-specific T cell stimulation and to release various cytokines. However, the pathogenesis of immunoparalysis may differ in various patient groups. It can develop in patients after systemic hyperinflammation induced by gastrointestinal translocation of endotoxin (lipopolysaccharide, LPS) or sepsis, as well as in patients without preceding systemic inflammation but primary anti-inflammation, for instance induced by sympathetic activation. To further elucidate the syndrome, we compared endotoxin tolerance as a model of immunoparalysis after systemic hyperinflammation versus interleukin-10 (IL-10) treatment as a model of primarily anti-inflammation-induced immunoparalysis. In vitro priming of peripheral blood mononuclear cells with either LPS or IL-10 for 24 h led to a strongly or moderately diminished LPS-induced tumor necrosis factor-alpha (TNF-alpha) production, compared to unprimed controls, respectively. Furthermore, LPS-induced reduction of TNF-alpha production capacity persisted over the following days whereas IL-10-primed monocytes rapidly recovered. Similarly, in contrast to persistently diminished MHC class II expression in LPS-treated monocytes, IL-10 only transiently downregulated these molecules. Consequently, in contrast to IL-10-primed monocytes, LPS-primed monocytes were greatly impaired in their capacity to induce antigen-specific T cell proliferation and IFN-gamma production. These data indicate that LPS priming provokes a more profound modulation of monocyte function than IL-10 priming, raising the question of possible variations in the clinical course of immunoparalysis, dependent on its pathogenesis.

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Research paper thumbnail of Transfer of Autologous Gene-modified T Cells in HIV-infected Patients with Advanced Immunodeficiency and Drug-resistant Virus

Molecular Therapy, 2007

Drug toxicity and viral resistance limit the long-term efficacy of antiviral drug treatment for h... more Drug toxicity and viral resistance limit the long-term efficacy of antiviral drug treatment for human immunodeficiency virus (HIV) infection. Thus, alternative therapies need to be explored. We tested the infusion of T lymphocytes transduced with a retroviral vector (M87o) that expresses an HIV entry-inhibitory peptide (maC46). Gene-modified autologous T cells were infused into ten HIV-infected patients with advanced disease and multidrug-resistant virus during anti-retroviral combination therapy. T-cell infusions were tolerated well, with no severe side effects. A significant increase of CD4 counts was observed after infusion. At the end of the 1-year follow-up, the CD4 counts of all patients were still around or above baseline. Gene-modified cells could be detected in peripheral blood, lymph nodes, and bone marrow throughout the 1-year follow-up, and marking levels correlated with the cell dose. No significant changes of viral load were observed during the first 4 months. Four of the seven patients who changed their antiviral drug regimen thereafter responded with a significant decline in plasma viral load. In conclusion, the transfer of gene-modified cells was safe, led to sustained levels of gene marking, and may improve immune competence in HIV-infected patients with advanced disease and multidrug-resistant virus.

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Research paper thumbnail of Heterozygous Arg753Gln polymorphism of human TLR-2 impairs immune activation by Borrelia burgdorferi and protects from late stage Lyme disease

The Journal of Immunology, 2006

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Improving the in vitro antigen specific T cell proliferation assay: the use of interferon-alpha to elicit antigen specific stimulation and decrease bystander proliferation

Journal of Immunological Methods, 2001

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Impaired immune function in patients with alcoholic liver cirrhosis(ALC) due to active cytomegalovirus (CMV) infection

Journal of Hepatology, 2000

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Research paper thumbnail of Correlation between energy expenditure, nutrient intake, malnutrition and activation of the inflammatory system in patients with hepatic cirrhosis

Journal of Hepatology, 2001

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Activation of immune functions in patients with liver cirrhosis (LC) by selective gut decontamination (SGD)

Journal of Hepatology, 2001

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Immunologic Effector Mechanisms of a Standardized Mistletoe Extract on the Function of Human Monocytes and Lymphocytes in vitro, ex vivo, and in vivo

Journal of Clinical Immunology, 2006

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Compound deletion of the rhoGAP C1 and V2 vasopressin receptor genes in a patient with nephrogenic diabetes insipidus

Human Mutation, 1999

The function of small GTPases is fine-tuned by a complex network of regulatory proteins such as G... more The function of small GTPases is fine-tuned by a complex network of regulatory proteins such as GTPase-activating proteins. The C1 gene at Xq28 encodes a protein assumed to function as a Rho GTPase-activating protein (rhoGAP). Characterization of the molecular defect causing X-linked nephrogenic diabetes insipidus (NDI) in a patient revealed a submicroscopic deletion of a 21.5-kb genomic fragment encompassing the entire arginine-vasopressin V2 receptor gene (AVPR2) and most of the C1 gene locus. In the absence of detailed information about the physiological relevance and specific functions of rhoGAP C1, a thorough clinical and laboratory investigation of the patient was performed. Besides clearly defined NDI symptoms caused by deletion of the AVPR2 gene, no major morphological abnormalities as determined by physical examination, radiography, ultrasound, and computed tomographic scan were detected. Extensive analysis of blood chemical, enzyme, and hormone values over a period of 16 years showed no deviations from normal ranges. On the basis of our observations, the rhoGAP C1 protein is not essential for normal development in the human. Because of a predominant expression pattern of the C1 gene in hematopoietic cells, we focused on immunologic and hematologic laboratory parameters of the affected boy and the mother who was found to be heterozygous. Differential white cell counts, including lymphocyte typing, determination of lymphokines, cytokines, and immunoglobulins, as well as numerous leukocyte function tests, showed no pathological findings. Therefore, we postulate that the loss of rhoGAP C1 function is most likely compensated by other members of the GAP family.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Clinical manifestation of mannose-binding lectin deficiency in adults independent of concomitant immunodeficiency

Human Immunology, 2009

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