Wiesława Klimek-Piotrowska - Academia.edu (original) (raw)

Papers by Wiesława Klimek-Piotrowska

Annals of Anatomy - Anatomischer Anzeiger, 2017

The mitral isthmus is a part of the postero-inferior area of the lateral left atrial wall located... more The mitral isthmus is a part of the postero-inferior area of the lateral left atrial wall located between the mitral annulus and the left inferior pulmonary vein ostium. Linear ablation lesions are created within the mitral isthmus for the invasive treatment of left atrial arrhythmias. However, the anatomy of this region is not fully understood. The aim of this study has been to provide a detailed morphometric description of the mitral isthmus region and to propose another possible isthmus within the investigated heart area that may serve as a potential new ablation target. Two hundred autopsied, non-atrial fibrillation hearts (23.5% deriving from females) whose donors were a mean of 47.6±17.6years old were investigated. We macroscopically assessed the anatomy of the postero-inferior area of the lateral left atrial wall. The mean mitral isthmus length was 28.8±7.0mm and was significantly longer than the left atrial appendage (LAA) isthmus (14.2±4.8mm) (p=.00). The distance between the LAA orifice and the left inferior pulmonary vein ostium (18.4±4.8mm) was longer than the LAA isthmus (p=.00) and shorter than the mitral isthmus (p=.00). The LAA isthmus was longer in hearts with a common left pulmonary vein (p=.037). In 65.5% of all cases the area between the right and left mitral isthmus lines was completely smooth. In the remaining hearts, crevices and diverticula (18.0%), intertrabecular recesses (7.0%), trabecular bridges (3.5%), or co-existence of these structures (6%) could be observed. The LAA isthmus line was smooth in 95.5% of all cases, with only small crevices in the remaining 4.5%. In conclusion, regardless of the anatomical variants of the left-sided pulmonary veins, the mitral isthmus area is quite uniform in size. The LAA isthmus is considerably shorter than the mitral isthmus. The mitral isthmus line has many unwanted structures that may entrap the catheter, which is not the case for the LAA isthmus. We proposed the LAA isthmus line for potential clinical use.

EP Europace, 2017

The aim of this study was to assess the spatial relationship of blood vessels and the thickness o... more The aim of this study was to assess the spatial relationship of blood vessels and the thickness of the atrial wall within the mitral isthmus line. A total of 200 randomly selected autopsied adult human hearts (Caucasian) were examined. The mitral isthmus line was cut longitudinally and the thickness of the left atrial wall was measured. The blood vessels within the isthmus were identified and their relationship with the endocardial surface (ES), mitral annulus (MA), and the left inferior pulmonary vein (LIPV) ostium was assessed. The mean myocardial thickness in the upper, middle, and lower 1/3 of the mitral isthmus section were 1.9 ± 1.0, 3.0 ± 1.5, and 2.7 ± 1.3 mm, respectively. The great cardiac vein (GCV) was present within the isthmus in 98.0%, the left circumflex artery (LCx) in 57.0%, and the Marshall vein in 35.0% of all hearts. The GCV was located 4.5 ± 2.2 mm from the ES, 7.3 ± 5.3 mm from the MA, and 24.3 ± 7.3 mm from the LIPV. The LCx was situated 3.8 ± 2.3 mm from the ES, 7.9 ± 5.1 mm from the MA, and 25.3 ± 8.0 mm from the LIPV. We were able to detect eight different patterns of GCV and LCx mutual arrangement within the mitral isthmus line. The myocardium is the thinnest in the upper 1/3 sector, and the blood vessels are mainly located in the middle and lower 1/3. In 49.1%, the LCx is situated at a distance of less than 3 mm from the ES. In 55.3%, the LCx is located between the GCV and ES of the left atrium.

The New England journal of medicine, Jan 15, 2016

PLOS ONE, 2016

The aim of this study was to provide useful information about the cavotricuspid isthmus (CTI) and... more The aim of this study was to provide useful information about the cavotricuspid isthmus (CTI) and surrounding areas morphology, which may help to plan CTI radio-frequency ablation. We examined 140 autopsied human hearts from Caucasian individuals of both sexes (29.3% females) with a mean age of 49.1±17.2 years. We macroscopically investigated the lower part of the right atrium, the CTI, the inferior vena cava ostium and the terminal crest. The paraseptal isthmus (18.5±4.0 mm) was significantly shorter than the central isthmus (p<0.0001), and the central isthmus (24.0±4.2 mm) was significantly shorter than the inferolateral isthmus (29.3±4.9 mm) (p<0.0001). Heart weight was positively correlated with all isthmus diameters. Three different sectors of CTI were distinguished: anterior, middle and posterior. The middle sector of the CTI presented a different morphology: trabeculae (N = 87; 62.1%), intertrabecular recesses (N = 35; 25.0%) and trabecular bridges (N = 18; 12.9%). A single sub-Eustachian recess was present in 48.6% of hearts (N = 68), and a double recess was present in 2.9% of hearts (N = 4) with mean depth = 5.6±1.8mm and diameter = 7.1±3.4mm. The morphology of the distal terminal crest was varied; 10 patterns of the distal terminal crest ramifications were noted. There were no statistically significant differences in any of the investigated CTI parameters between groups with different types of terminal crest ramifications. The presence of intertrabecular recesses (25.0%), trabecular bridges (12.9%) and sub-Eustachian recesses (48.6%) within the CTI can make ablation more difficult. We have presented the macroscopic patterns of final ramifications of the terminal crest within the quadrilateral CTI area.

Journal of Anatomy, 2016

Recent extensive progress in invasive cardiac procedures has triggered a wave of dozens of heart ... more Recent extensive progress in invasive cardiac procedures has triggered a wave of dozens of heart morphometric anatomical studies that are carried out largely using autopsied samples fixed in formaldehyde solution prior to observations and measurements. In reality, very little is known about changes in heart tissue dimensions during fixation. The aim of this study was therefore to investigate how fixation affects the dimensions of cardiac tissue, and if different types and concentrations of reagents affect this phenomenon. A total of 40 pig heart samples were investigated, and seven different measuring sites were permanently marked in every heart prior to fixation. Four study groups (n = 10 each) were assembled that differed only in concentration and the type of fixative: (i) 2% formaldehyde solution; (ii) 4% formaldehyde solution (formalin); (iii) 10% formaldehyde solution; (iv) alcoholic formalin. The samples were measured before and after fixation at the following time points: 24 h, 72 h and 168 h. It was found that different fixatives significantly affected different parameters. Almost all of the heart dimensions that were measured stabilized after 24 h; later changes were statistically insignificant in the point-to-point comparison. Change in the length of the interatrial septum surface was not altered significantly in any of the fixatives after 24 h of preservation. It was found that 10% formaldehyde increased the thickness of muscular tissue only after 24 h; this thickening was reduced after 72 h and was insignificant at 168 h. Other heart parameters in this group do not present significant changes over the entire fixation time duration. In conclusion, the 10% formaldehyde phosphate-buffered solution appeared to be the best fixative among the fixatives that were studied for cardiac morphometric purposes; this solution caused the smallest changes in tissue dimensions. Measurements should be obtained at least after 1 week of preservation when most parameters exhibit the smallest changes compared with the non-preserved samples.

Annals of Anatomy - Anatomischer Anzeiger, 2016

Clinical anatomy of the interatrial septum is treacherous, difficult and its unfamiliarity can ca... more Clinical anatomy of the interatrial septum is treacherous, difficult and its unfamiliarity can cause many serious complications. This work aims to create an anatomical map of the true interatrial septum. An appreciation of the anatomical situation is essential for safe and efficacious transseptal access from the right atrium to the left heart chambers. Examination of 135 autopsied human hearts (Caucasian) of both sexes (28% females) aged from 19 to 94 years old (47.±18.2) with BMI=27.1±6.0kg/m2 was conducted. Focus was specifically targeted on the assessment of the fossa ovalis, patent foramen ovale (PFO), and right-sided septal pouch (RSP) morphology. Mean values of cranio-caudal and antero-posterior fossa ovalis diameters were 12.1±3.6mm and 14.1±3.6mm respectively. The fossa ovalis was situated an average of 10.1±4.4mm above the inferior vena cava ostium, 20.7±5.2mm from the right atrioventricular ring, and 12.6±5.2mm under the right atrium roof. Four types of fossa ovalis anatomy have been observed (smooth-56.3%, PFO-24.4%, RSP-11.9%, net-like formation-7.4%). The PFO mean channel length was 10.5±5.2mm. The tunnel-like PFO (channel length ≥12mm) was observed in 8.9% of specimens. The RSP was observed in 11.9% of specimens (with mean depth=6.3±3.8mm) and was directed apex upward in all observed specimens (may imitate the PFO channel). The fossa ovalis/interatrial septum surface area ratio was 18.3±9.0%. (1) An anatomical map of the interatrial septum from the right atrial side was presented. (2) The RSP may imitate the PFO channel. (3) The "true" interatrial septum represents only about 20% of the whole interatrial septum area. (4) There is wide variation in the location and geometry of the fossa ovalis. (5) We could distinguish 4 different types of the fossa ovalis area.

PeerJ, 2016

Background. It is well known that the pulmonary veins (PVs), especially their myocardial sleeves ... more Background. It is well known that the pulmonary veins (PVs), especially their myocardial sleeves play a critical role in the initiation and maintenance of atrial fibrillation. Understanding the PV anatomy is crucial for the safety and efficacy of all procedures performed on PVs. The aim of this study was to present normal distal PV anatomy and to create a juxtaposition of all PV ostium variants. Methods. A total of 130 randomly selected autopsied adult human hearts (Caucasian) were examined. The number of PVs ostia was evaluated and their diameter was measured. The ostium-to-last-tributary distance and macroscopic presence of myocardial sleeves were also evaluated. Results. Five hundred forty-one PV ostia were identified. Four classical PV ostia patterns (two left and two right PVs) were observed in 70.8% of all cases. The most common variant was the classical pattern with additional middle right PV (19.2%), followed by the common ostium for the left superior and the inferior PVs (4.44%). Mean diameters of PV ostia (for the classical pattern) were: left superior = 13.8 ± 2.9 mm; left inferior = 13.3 ± 3.4 mm; right superior = 14.3 ± 2.9 mm; right inferior = 13.7 ± 3.3 mm. When present, the additional middle right PV ostium had the smallest PV ostium diameter in the heart (8.2 ± 4.1 mm). The mean ostium-to-last-tributary (closest to the atrium) distances were: left superior = 15.1 ± 4.6 mm; left inferior = 13.5 ± 4.0 mm; right superior = 11.8 ± 4.0 mm; right inferior = 11.0 ± 3.7 mm. There were no statistically significant differences between sexes in ostia diameters and ostium-to-last-tributary distances. Conclusion. Only 71% of the cases have four standard pulmonary veins. The middle right pulmonary vein is present in almost 20% of patients. Presented data can provide useful information for the clinicians during interventional procedures or radiologic examinations of PVs.

Anadolu Kardiyoloji Dergisi/The Anatolian Journal of Cardiology, 2015

According to our observations of 300 cadaver hearts, we can conclude that valves covering more th... more According to our observations of 300 cadaver hearts, we can conclude that valves covering more than 100% of the CSO are present in 2.5% of individuals and can make conventional CS cannulation difficult or even impossible .

Journal of neurosurgical sciences, 2016

Journal of neurosurgical sciences, Feb 1, 2017

Folia medica Cracoviensia, 2015

Based on the review of the current literature and our own experiences we reviewed the literature ... more Based on the review of the current literature and our own experiences we reviewed the literature paying most attention to human venous coronary system with special respect to coronary sinus - the largest vein of the heart. Many details still remain obscure, although most of modern procedures require deep anatomical knowledge on cardiac veins. Many of developmental remarks are still not clear, regarded to development of the vessels of the heart in association with origin of heart conductive system.

Folia medica Cracoviensia, 2015

The study was carried out on 80 human hearts obtained during autopsies. The vascular beds were fi... more The study was carried out on 80 human hearts obtained during autopsies. The vascular beds were filled with synthetic resin and next corroded. In all 80 hearts we have been found both: great, middle and small cardiac veins. In two out of 80 hearts we did not find oblique vein of the left atrium. Posterior vein of the left ventricle was double in four hearts. Right marginal vein was found in 5 hearts, left marginal vein was seen in 9 hearts. We also compared the dimensions of all of the above mentioned tributaries of the coronary sinus.

International Journal of Cardiology, 2016

The atrial septal pouch (SP) is a new anatomical entity within the interatrial septum. The left-s... more The atrial septal pouch (SP) is a new anatomical entity within the interatrial septum. The left-sided SP may be the source of thrombus and contribute to ischemic stroke. The aim of this study was to provide a detailed morphometric description of the SP. Two hundred autopsied hearts (23% deriving from females) with a mean age of 46.7±19.1years were investigated. We assessed the morphology of the interatrial septum. We obtained measurements and casts of the SPs, and we conducted histological staining of the left-sided SPs. Patent foramen ovale was observed in 25% of hearts. We found a left SP in 41.5%, right in 5.5% and a double SP in 5.5% of hearts. We found the patent foramen ovale (PFO) more often in younger hearts, and the SP and smooth septum were more prevalently found in older hearts (p=.0023). The mean volume of the left-sided SP was 0.31±0.11ml, which represented 13.6±9.4% (range: 3.1-44.9%) of the left atrial appendage volume. The SP shape resembled a cone or a cylinder with some smaller diverticula originating from the main body. The SP free wall was composed of two layers of endocardium, transverse muscle fibers and connective tissue. A left-sided SP was present in 47% of individuals. The SP arises as a result of PFO channel closure. The anatomy of left-sided SP may promote blood stasis and thrombus formation. The universal formula for SP volume was calculated.

Europace, 2016

The first aim of this study was to determine the size of the Koch&amp... more The first aim of this study was to determine the size of the Koch's triangle. The second one was to investigate relation between its dimensions and other individual-specific and heart-specific parameters as well as to create universal formula to estimate triangle dimensions based on these parameters. This study is a prospective one, presenting 120 randomly selected autopsied hearts dissected from adult humans (Caucasian) of both sexes (31.7% females), with mean age of 49.3 ± 17.4 years. The length of triangle sides and angles were measured and the triangle area was calculated as well. Sixteen additional heart parameters were measured in order to analyse potential relationship between the dimensions of Koch's triangle and other dimensions of the heart, using linear regression analysis. The mean (±SD) length of the anterior edge was approximated to 18.0 ± 3.8 mm, the posterior edge to 20.3 ± 4.3 mm, and the basal edge to 18.5 ± 4.0 mm. The average values of the apex angle, the Eustachian angle, and the septal leaflet angle were 58.0 ± 14.4°, 53.8 ± 10.6°, and 67.6 ± 14.4°, respectively. The mean value of the Koch's triangle area was 151.5 ± 55.8 mm(2). The 95th percentile of triangle's height (the distance from the apex to the coronary sinus) was 21.8 mm. Mean values and proportions of triangle's sides and angles were presented. Koch's triangle showed considerable individual variations in size. The dimensions of the triangle were strongly independent from individual-specific and heart-specific morphometric parameters; however, the maximum triangle's height can be estimated as 22 mm.

PeerJ, 2016

Background. The coronary venous system is an increasingly frequent target of minimally invasive c... more Background. The coronary venous system is an increasingly frequent target of minimally invasive cardiac procedures. The purpose of this paper is to assess the anatomical barriers in the right atrium to coronary sinus cannulation. Methods. We examined the anatomy of the right atrium, coronary sinus ostium, inferior and superior vena cava ostia in 110 randomly selected autopsied human hearts of both sexes (27% females; mean age 49.2 ± 17.5 years). Results. The Eustachian valve was present in 79 cases (71.8%) with mean height =4.9 ± 2.6 mm. The valve was perforated in 11 cases (13.9%). It is typically too small to hinder the coronary sinus catheterization, but in some cases (about 2%) a significantly protruding valve may be an obstacle. Chiari's network (4.6%) is not a barrier to catheter entry into the right atrium but may significantly impede further catheter manipulations inside the heart venous system. A typical Thebesian valve leaves enough space for the passage of the standard catheter to the coronary sinus. Discussion. Detailed anatomy of various anatomical structures within the right atrium that could play a potential role in coronary sinus cannulation is discussed.

Introduction: So far, very little data on pediatric obesity has been published in Poland, althoug... more Introduction: So far, very little data on pediatric obesity has been published in Poland, although the problem seems to be growing. Objective: To investigate present prevalence of overweight and obesity among schoolchildren using CDC (Center of Disease Control and Prevention) criteria in Krakow and to compare how it has changed between the years 2001 and 2013. Our aim was to establish the magnitude of this rising problem within the last decade.

Anatomical Science International, 2015

The circle of Willis (CW) is an anastomotic system of arteries located at the base of the brain. ... more The circle of Willis (CW) is an anastomotic system of arteries located at the base of the brain. The aim of the study was to evaluate the anatomic configuration of the CW in the Polish population and to compare results with previously conducted research. Brains were obtained from 100 recently deceased human adults, and the diameters of cerebral vessels were measured using a slide caliper. Cerebral vessels were observed, paying attention to their origin, diameter, typical configuration and variations. Twenty-seven percent of cases presented the typical literature pattern. The remaining 73 % of all cases were atypical; in 16 % the CW was incomplete and in 57 % complete. Atypical findings involved the posterior communicating artery (PcomA), 62 %; anterior communicating artery (AcomA), 22 %; anterior cerebral artery (ACA), 14 %; posterior cerebral artery (PCA), 8 %. The most common variations were bilateral hypoplastic PcomAs (27 % of cases) and unilateral hypoplastic PcomAs (19 % of cases). Only 9 of the 22 types of CW variations classified previously in the literature were observed, and 26 variations (36 cases) in our study were labeled as 'other' type. Mean diameter values for typical CW patterns were internal carotid artery = 3.6 mm, ACA = 2.3 mm, AcomA = 1.9 mm, PCA = 2.2 mm and PcomA = 1.4 mm. Circle of Willis variations have a large impact on clinical practice. This study shows many rare variations that should be taken into consideration to avoid any unexpected complications during surgical procedures involving cerebral vessels.

International Journal of Cardiology, 2015

The coronary sinus ostium (CSO) is covered by the Thebesian valve (ThebV), which has a variable s... more The coronary sinus ostium (CSO) is covered by the Thebesian valve (ThebV), which has a variable shape when assessed subjectively. The ThebV is an anatomical barrier during CS cannulation, which may be complicated due to the valves' size. The types of valves are: cord, remnant, semilunar, fold, and mesh/fenestrated. The ThebV can be visible using multisliced computed tomography (MSCT), however, this method cannot show the ThebV's morphological shape, only its size. 301 randomly selected autopsied human hearts were examined. The shape of the valve was subjectively assessed, whereas the ThebV height (H) and the CSO diameter (D) were measured. The H/D-Ratio was computed as the ThebV height divided by the CSO diameter, afterwards k-means cluster analysis was performed to estimate H/D-Ratio's range of values between valves. MSCT scans from 114 patients that underwent CSO cannulation were objectively evaluated based on similar measured parameters in accordance with received H/D-Ratio values. Boundaries of ratio evaluations between remnant and semilunar, and semilunar and fold types were 0.35 and 0.65 respectively. In MSCT scans, the ThebV was recorded in 61 cases (remnant=5.3%, semilunar=24.6%, fold=16.7%, cord=0.0%, mesh/fenestrated=7.9%). Except for the remnant and cord types, the other types appear similarly as in the cadaveric and MSCT studies. There were no differences between ThebV height and the CSO diameter in cadavers and MSCT studies. The H/D-Ratio can be useful in assessing ThebV shape as visualized in MSCT. We give threshold values for the H/D-Ratio which easily allow the ThebV shape to be determined.

Annals of Anatomy - Anatomischer Anzeiger, 2017

The mitral isthmus is a part of the postero-inferior area of the lateral left atrial wall located... more The mitral isthmus is a part of the postero-inferior area of the lateral left atrial wall located between the mitral annulus and the left inferior pulmonary vein ostium. Linear ablation lesions are created within the mitral isthmus for the invasive treatment of left atrial arrhythmias. However, the anatomy of this region is not fully understood. The aim of this study has been to provide a detailed morphometric description of the mitral isthmus region and to propose another possible isthmus within the investigated heart area that may serve as a potential new ablation target. Two hundred autopsied, non-atrial fibrillation hearts (23.5% deriving from females) whose donors were a mean of 47.6±17.6years old were investigated. We macroscopically assessed the anatomy of the postero-inferior area of the lateral left atrial wall. The mean mitral isthmus length was 28.8±7.0mm and was significantly longer than the left atrial appendage (LAA) isthmus (14.2±4.8mm) (p=.00). The distance between the LAA orifice and the left inferior pulmonary vein ostium (18.4±4.8mm) was longer than the LAA isthmus (p=.00) and shorter than the mitral isthmus (p=.00). The LAA isthmus was longer in hearts with a common left pulmonary vein (p=.037). In 65.5% of all cases the area between the right and left mitral isthmus lines was completely smooth. In the remaining hearts, crevices and diverticula (18.0%), intertrabecular recesses (7.0%), trabecular bridges (3.5%), or co-existence of these structures (6%) could be observed. The LAA isthmus line was smooth in 95.5% of all cases, with only small crevices in the remaining 4.5%. In conclusion, regardless of the anatomical variants of the left-sided pulmonary veins, the mitral isthmus area is quite uniform in size. The LAA isthmus is considerably shorter than the mitral isthmus. The mitral isthmus line has many unwanted structures that may entrap the catheter, which is not the case for the LAA isthmus. We proposed the LAA isthmus line for potential clinical use.

EP Europace, 2017

The aim of this study was to assess the spatial relationship of blood vessels and the thickness o... more The aim of this study was to assess the spatial relationship of blood vessels and the thickness of the atrial wall within the mitral isthmus line. A total of 200 randomly selected autopsied adult human hearts (Caucasian) were examined. The mitral isthmus line was cut longitudinally and the thickness of the left atrial wall was measured. The blood vessels within the isthmus were identified and their relationship with the endocardial surface (ES), mitral annulus (MA), and the left inferior pulmonary vein (LIPV) ostium was assessed. The mean myocardial thickness in the upper, middle, and lower 1/3 of the mitral isthmus section were 1.9 ± 1.0, 3.0 ± 1.5, and 2.7 ± 1.3 mm, respectively. The great cardiac vein (GCV) was present within the isthmus in 98.0%, the left circumflex artery (LCx) in 57.0%, and the Marshall vein in 35.0% of all hearts. The GCV was located 4.5 ± 2.2 mm from the ES, 7.3 ± 5.3 mm from the MA, and 24.3 ± 7.3 mm from the LIPV. The LCx was situated 3.8 ± 2.3 mm from the ES, 7.9 ± 5.1 mm from the MA, and 25.3 ± 8.0 mm from the LIPV. We were able to detect eight different patterns of GCV and LCx mutual arrangement within the mitral isthmus line. The myocardium is the thinnest in the upper 1/3 sector, and the blood vessels are mainly located in the middle and lower 1/3. In 49.1%, the LCx is situated at a distance of less than 3 mm from the ES. In 55.3%, the LCx is located between the GCV and ES of the left atrium.

The New England journal of medicine, Jan 15, 2016

PLOS ONE, 2016

The aim of this study was to provide useful information about the cavotricuspid isthmus (CTI) and... more The aim of this study was to provide useful information about the cavotricuspid isthmus (CTI) and surrounding areas morphology, which may help to plan CTI radio-frequency ablation. We examined 140 autopsied human hearts from Caucasian individuals of both sexes (29.3% females) with a mean age of 49.1±17.2 years. We macroscopically investigated the lower part of the right atrium, the CTI, the inferior vena cava ostium and the terminal crest. The paraseptal isthmus (18.5±4.0 mm) was significantly shorter than the central isthmus (p<0.0001), and the central isthmus (24.0±4.2 mm) was significantly shorter than the inferolateral isthmus (29.3±4.9 mm) (p<0.0001). Heart weight was positively correlated with all isthmus diameters. Three different sectors of CTI were distinguished: anterior, middle and posterior. The middle sector of the CTI presented a different morphology: trabeculae (N = 87; 62.1%), intertrabecular recesses (N = 35; 25.0%) and trabecular bridges (N = 18; 12.9%). A single sub-Eustachian recess was present in 48.6% of hearts (N = 68), and a double recess was present in 2.9% of hearts (N = 4) with mean depth = 5.6±1.8mm and diameter = 7.1±3.4mm. The morphology of the distal terminal crest was varied; 10 patterns of the distal terminal crest ramifications were noted. There were no statistically significant differences in any of the investigated CTI parameters between groups with different types of terminal crest ramifications. The presence of intertrabecular recesses (25.0%), trabecular bridges (12.9%) and sub-Eustachian recesses (48.6%) within the CTI can make ablation more difficult. We have presented the macroscopic patterns of final ramifications of the terminal crest within the quadrilateral CTI area.

Journal of Anatomy, 2016

Recent extensive progress in invasive cardiac procedures has triggered a wave of dozens of heart ... more Recent extensive progress in invasive cardiac procedures has triggered a wave of dozens of heart morphometric anatomical studies that are carried out largely using autopsied samples fixed in formaldehyde solution prior to observations and measurements. In reality, very little is known about changes in heart tissue dimensions during fixation. The aim of this study was therefore to investigate how fixation affects the dimensions of cardiac tissue, and if different types and concentrations of reagents affect this phenomenon. A total of 40 pig heart samples were investigated, and seven different measuring sites were permanently marked in every heart prior to fixation. Four study groups (n = 10 each) were assembled that differed only in concentration and the type of fixative: (i) 2% formaldehyde solution; (ii) 4% formaldehyde solution (formalin); (iii) 10% formaldehyde solution; (iv) alcoholic formalin. The samples were measured before and after fixation at the following time points: 24 h, 72 h and 168 h. It was found that different fixatives significantly affected different parameters. Almost all of the heart dimensions that were measured stabilized after 24 h; later changes were statistically insignificant in the point-to-point comparison. Change in the length of the interatrial septum surface was not altered significantly in any of the fixatives after 24 h of preservation. It was found that 10% formaldehyde increased the thickness of muscular tissue only after 24 h; this thickening was reduced after 72 h and was insignificant at 168 h. Other heart parameters in this group do not present significant changes over the entire fixation time duration. In conclusion, the 10% formaldehyde phosphate-buffered solution appeared to be the best fixative among the fixatives that were studied for cardiac morphometric purposes; this solution caused the smallest changes in tissue dimensions. Measurements should be obtained at least after 1 week of preservation when most parameters exhibit the smallest changes compared with the non-preserved samples.

Annals of Anatomy - Anatomischer Anzeiger, 2016

Clinical anatomy of the interatrial septum is treacherous, difficult and its unfamiliarity can ca... more Clinical anatomy of the interatrial septum is treacherous, difficult and its unfamiliarity can cause many serious complications. This work aims to create an anatomical map of the true interatrial septum. An appreciation of the anatomical situation is essential for safe and efficacious transseptal access from the right atrium to the left heart chambers. Examination of 135 autopsied human hearts (Caucasian) of both sexes (28% females) aged from 19 to 94 years old (47.±18.2) with BMI=27.1±6.0kg/m2 was conducted. Focus was specifically targeted on the assessment of the fossa ovalis, patent foramen ovale (PFO), and right-sided septal pouch (RSP) morphology. Mean values of cranio-caudal and antero-posterior fossa ovalis diameters were 12.1±3.6mm and 14.1±3.6mm respectively. The fossa ovalis was situated an average of 10.1±4.4mm above the inferior vena cava ostium, 20.7±5.2mm from the right atrioventricular ring, and 12.6±5.2mm under the right atrium roof. Four types of fossa ovalis anatomy have been observed (smooth-56.3%, PFO-24.4%, RSP-11.9%, net-like formation-7.4%). The PFO mean channel length was 10.5±5.2mm. The tunnel-like PFO (channel length ≥12mm) was observed in 8.9% of specimens. The RSP was observed in 11.9% of specimens (with mean depth=6.3±3.8mm) and was directed apex upward in all observed specimens (may imitate the PFO channel). The fossa ovalis/interatrial septum surface area ratio was 18.3±9.0%. (1) An anatomical map of the interatrial septum from the right atrial side was presented. (2) The RSP may imitate the PFO channel. (3) The "true" interatrial septum represents only about 20% of the whole interatrial septum area. (4) There is wide variation in the location and geometry of the fossa ovalis. (5) We could distinguish 4 different types of the fossa ovalis area.

PeerJ, 2016

Background. It is well known that the pulmonary veins (PVs), especially their myocardial sleeves ... more Background. It is well known that the pulmonary veins (PVs), especially their myocardial sleeves play a critical role in the initiation and maintenance of atrial fibrillation. Understanding the PV anatomy is crucial for the safety and efficacy of all procedures performed on PVs. The aim of this study was to present normal distal PV anatomy and to create a juxtaposition of all PV ostium variants. Methods. A total of 130 randomly selected autopsied adult human hearts (Caucasian) were examined. The number of PVs ostia was evaluated and their diameter was measured. The ostium-to-last-tributary distance and macroscopic presence of myocardial sleeves were also evaluated. Results. Five hundred forty-one PV ostia were identified. Four classical PV ostia patterns (two left and two right PVs) were observed in 70.8% of all cases. The most common variant was the classical pattern with additional middle right PV (19.2%), followed by the common ostium for the left superior and the inferior PVs (4.44%). Mean diameters of PV ostia (for the classical pattern) were: left superior = 13.8 ± 2.9 mm; left inferior = 13.3 ± 3.4 mm; right superior = 14.3 ± 2.9 mm; right inferior = 13.7 ± 3.3 mm. When present, the additional middle right PV ostium had the smallest PV ostium diameter in the heart (8.2 ± 4.1 mm). The mean ostium-to-last-tributary (closest to the atrium) distances were: left superior = 15.1 ± 4.6 mm; left inferior = 13.5 ± 4.0 mm; right superior = 11.8 ± 4.0 mm; right inferior = 11.0 ± 3.7 mm. There were no statistically significant differences between sexes in ostia diameters and ostium-to-last-tributary distances. Conclusion. Only 71% of the cases have four standard pulmonary veins. The middle right pulmonary vein is present in almost 20% of patients. Presented data can provide useful information for the clinicians during interventional procedures or radiologic examinations of PVs.

Anadolu Kardiyoloji Dergisi/The Anatolian Journal of Cardiology, 2015

According to our observations of 300 cadaver hearts, we can conclude that valves covering more th... more According to our observations of 300 cadaver hearts, we can conclude that valves covering more than 100% of the CSO are present in 2.5% of individuals and can make conventional CS cannulation difficult or even impossible .

Journal of neurosurgical sciences, 2016

Journal of neurosurgical sciences, Feb 1, 2017

Folia medica Cracoviensia, 2015

Based on the review of the current literature and our own experiences we reviewed the literature ... more Based on the review of the current literature and our own experiences we reviewed the literature paying most attention to human venous coronary system with special respect to coronary sinus - the largest vein of the heart. Many details still remain obscure, although most of modern procedures require deep anatomical knowledge on cardiac veins. Many of developmental remarks are still not clear, regarded to development of the vessels of the heart in association with origin of heart conductive system.

Folia medica Cracoviensia, 2015

The study was carried out on 80 human hearts obtained during autopsies. The vascular beds were fi... more The study was carried out on 80 human hearts obtained during autopsies. The vascular beds were filled with synthetic resin and next corroded. In all 80 hearts we have been found both: great, middle and small cardiac veins. In two out of 80 hearts we did not find oblique vein of the left atrium. Posterior vein of the left ventricle was double in four hearts. Right marginal vein was found in 5 hearts, left marginal vein was seen in 9 hearts. We also compared the dimensions of all of the above mentioned tributaries of the coronary sinus.

International Journal of Cardiology, 2016

The atrial septal pouch (SP) is a new anatomical entity within the interatrial septum. The left-s... more The atrial septal pouch (SP) is a new anatomical entity within the interatrial septum. The left-sided SP may be the source of thrombus and contribute to ischemic stroke. The aim of this study was to provide a detailed morphometric description of the SP. Two hundred autopsied hearts (23% deriving from females) with a mean age of 46.7±19.1years were investigated. We assessed the morphology of the interatrial septum. We obtained measurements and casts of the SPs, and we conducted histological staining of the left-sided SPs. Patent foramen ovale was observed in 25% of hearts. We found a left SP in 41.5%, right in 5.5% and a double SP in 5.5% of hearts. We found the patent foramen ovale (PFO) more often in younger hearts, and the SP and smooth septum were more prevalently found in older hearts (p=.0023). The mean volume of the left-sided SP was 0.31±0.11ml, which represented 13.6±9.4% (range: 3.1-44.9%) of the left atrial appendage volume. The SP shape resembled a cone or a cylinder with some smaller diverticula originating from the main body. The SP free wall was composed of two layers of endocardium, transverse muscle fibers and connective tissue. A left-sided SP was present in 47% of individuals. The SP arises as a result of PFO channel closure. The anatomy of left-sided SP may promote blood stasis and thrombus formation. The universal formula for SP volume was calculated.

Europace, 2016

The first aim of this study was to determine the size of the Koch&amp... more The first aim of this study was to determine the size of the Koch's triangle. The second one was to investigate relation between its dimensions and other individual-specific and heart-specific parameters as well as to create universal formula to estimate triangle dimensions based on these parameters. This study is a prospective one, presenting 120 randomly selected autopsied hearts dissected from adult humans (Caucasian) of both sexes (31.7% females), with mean age of 49.3 ± 17.4 years. The length of triangle sides and angles were measured and the triangle area was calculated as well. Sixteen additional heart parameters were measured in order to analyse potential relationship between the dimensions of Koch's triangle and other dimensions of the heart, using linear regression analysis. The mean (±SD) length of the anterior edge was approximated to 18.0 ± 3.8 mm, the posterior edge to 20.3 ± 4.3 mm, and the basal edge to 18.5 ± 4.0 mm. The average values of the apex angle, the Eustachian angle, and the septal leaflet angle were 58.0 ± 14.4°, 53.8 ± 10.6°, and 67.6 ± 14.4°, respectively. The mean value of the Koch's triangle area was 151.5 ± 55.8 mm(2). The 95th percentile of triangle's height (the distance from the apex to the coronary sinus) was 21.8 mm. Mean values and proportions of triangle's sides and angles were presented. Koch's triangle showed considerable individual variations in size. The dimensions of the triangle were strongly independent from individual-specific and heart-specific morphometric parameters; however, the maximum triangle's height can be estimated as 22 mm.

PeerJ, 2016

Background. The coronary venous system is an increasingly frequent target of minimally invasive c... more Background. The coronary venous system is an increasingly frequent target of minimally invasive cardiac procedures. The purpose of this paper is to assess the anatomical barriers in the right atrium to coronary sinus cannulation. Methods. We examined the anatomy of the right atrium, coronary sinus ostium, inferior and superior vena cava ostia in 110 randomly selected autopsied human hearts of both sexes (27% females; mean age 49.2 ± 17.5 years). Results. The Eustachian valve was present in 79 cases (71.8%) with mean height =4.9 ± 2.6 mm. The valve was perforated in 11 cases (13.9%). It is typically too small to hinder the coronary sinus catheterization, but in some cases (about 2%) a significantly protruding valve may be an obstacle. Chiari's network (4.6%) is not a barrier to catheter entry into the right atrium but may significantly impede further catheter manipulations inside the heart venous system. A typical Thebesian valve leaves enough space for the passage of the standard catheter to the coronary sinus. Discussion. Detailed anatomy of various anatomical structures within the right atrium that could play a potential role in coronary sinus cannulation is discussed.

Introduction: So far, very little data on pediatric obesity has been published in Poland, althoug... more Introduction: So far, very little data on pediatric obesity has been published in Poland, although the problem seems to be growing. Objective: To investigate present prevalence of overweight and obesity among schoolchildren using CDC (Center of Disease Control and Prevention) criteria in Krakow and to compare how it has changed between the years 2001 and 2013. Our aim was to establish the magnitude of this rising problem within the last decade.

Anatomical Science International, 2015

The circle of Willis (CW) is an anastomotic system of arteries located at the base of the brain. ... more The circle of Willis (CW) is an anastomotic system of arteries located at the base of the brain. The aim of the study was to evaluate the anatomic configuration of the CW in the Polish population and to compare results with previously conducted research. Brains were obtained from 100 recently deceased human adults, and the diameters of cerebral vessels were measured using a slide caliper. Cerebral vessels were observed, paying attention to their origin, diameter, typical configuration and variations. Twenty-seven percent of cases presented the typical literature pattern. The remaining 73 % of all cases were atypical; in 16 % the CW was incomplete and in 57 % complete. Atypical findings involved the posterior communicating artery (PcomA), 62 %; anterior communicating artery (AcomA), 22 %; anterior cerebral artery (ACA), 14 %; posterior cerebral artery (PCA), 8 %. The most common variations were bilateral hypoplastic PcomAs (27 % of cases) and unilateral hypoplastic PcomAs (19 % of cases). Only 9 of the 22 types of CW variations classified previously in the literature were observed, and 26 variations (36 cases) in our study were labeled as 'other' type. Mean diameter values for typical CW patterns were internal carotid artery = 3.6 mm, ACA = 2.3 mm, AcomA = 1.9 mm, PCA = 2.2 mm and PcomA = 1.4 mm. Circle of Willis variations have a large impact on clinical practice. This study shows many rare variations that should be taken into consideration to avoid any unexpected complications during surgical procedures involving cerebral vessels.

International Journal of Cardiology, 2015

The coronary sinus ostium (CSO) is covered by the Thebesian valve (ThebV), which has a variable s... more The coronary sinus ostium (CSO) is covered by the Thebesian valve (ThebV), which has a variable shape when assessed subjectively. The ThebV is an anatomical barrier during CS cannulation, which may be complicated due to the valves' size. The types of valves are: cord, remnant, semilunar, fold, and mesh/fenestrated. The ThebV can be visible using multisliced computed tomography (MSCT), however, this method cannot show the ThebV's morphological shape, only its size. 301 randomly selected autopsied human hearts were examined. The shape of the valve was subjectively assessed, whereas the ThebV height (H) and the CSO diameter (D) were measured. The H/D-Ratio was computed as the ThebV height divided by the CSO diameter, afterwards k-means cluster analysis was performed to estimate H/D-Ratio's range of values between valves. MSCT scans from 114 patients that underwent CSO cannulation were objectively evaluated based on similar measured parameters in accordance with received H/D-Ratio values. Boundaries of ratio evaluations between remnant and semilunar, and semilunar and fold types were 0.35 and 0.65 respectively. In MSCT scans, the ThebV was recorded in 61 cases (remnant=5.3%, semilunar=24.6%, fold=16.7%, cord=0.0%, mesh/fenestrated=7.9%). Except for the remnant and cord types, the other types appear similarly as in the cadaveric and MSCT studies. There were no differences between ThebV height and the CSO diameter in cadavers and MSCT studies. The H/D-Ratio can be useful in assessing ThebV shape as visualized in MSCT. We give threshold values for the H/D-Ratio which easily allow the ThebV shape to be determined.