Wilfrido Arrúa - Academia.edu (original) (raw)

Papers by Wilfrido Arrúa

Research paper thumbnail of Efectos adversos asociados a las vacunas COVID-19 en funcionarios y estudiantes de la Facultad de Ciencias Químicas de la UNA, año 2021

Revista de la Sociedad científica del Paraguay, May 31, 2023

Artículo publicado en acceso abierto bajo Licencia Creative Commons. Efectos adversos asociados a... more Artículo publicado en acceso abierto bajo Licencia Creative Commons. Efectos adversos asociados a las vacunas COVID-19 en funcionarios y estudiantes de la Facultad de Ciencias Químicas de la UNA, año 2021 Adverse effects associated with COVID-19 vaccines in officials and students of the Faculty of Chemical Sciences of UNA, year 2021

Research paper thumbnail of In Vivo Antidepressant-Like Effect Assessment of Two Aloysia Species in Mice and LCMS Chemical Characterization of Ethanol Extract

Molecules

Medicinal plants belonging to the Verbenaceae family demonstrated antidepressant effects in precl... more Medicinal plants belonging to the Verbenaceae family demonstrated antidepressant effects in preclinical studies. Depression is one of the largest contributors to the global health burden of all countries. Plants from the Aloysia genus are traditionally used for affective disorders, and some of them have proven anxiolytic and antidepressant activity. The aim of this work was to evaluate the antidepressant effect of the ethanolic extract of Aloysia gratissima var. gratissima (Agg) and Aloysia virgata var. platyphylla (Avp) in mice. A tail suspension test (TST) and forced swimming test (FST) were conducted after three doses in a period of 24 h and after 7 days of treatment. Imipramine was used as an antidepressant drug. The main results demonstrated that Agg extract reduced the immobility time in mice treated orally for 7 consecutive days when compared to the control group (reduced by about 77%, imipramine 70%). Animals treated with three doses of Avp in a 24-h period had reduced immob...

Research paper thumbnail of The antihypertensive and diuretic effect of crude root extract and saponins from Solanum sisymbriifolium Lam., in L-NAME-induced hypertension in rats

Journal of Ethnopharmacology

Research paper thumbnail of Determinación de parámetros bioquímicos de animales del Bioterio de la Facultad de Ciencias Químicas de la Universidad Nacional de Asunción

Revista de la Sociedad científica del Paraguay, May 31, 2023

Artículo publicado en acceso abierto bajo Licencia Creative Commons.

Research paper thumbnail of Attenuation of pain and inflammation induced in mice treated orally with crude extract of Aloysia polystachya (Griseb.) Moldenke (Verbenaceae)

Journal of Applied Pharmaceutical Science

The objective of this work was to determine the analgesic and anti-inflammatory activity of Aloys... more The objective of this work was to determine the analgesic and anti-inflammatory activity of Aloysia polystachya (Griseb.) Moldenke (Verbenaceae) in experimental models of acute pain and inflammation in mice. Methods of pain induced by caudal pressure (Randall-Selitto), chemical stimulation (acetic acid or the writhing test), and thermal stimulation (hot plate) were used to study analgesic effects. Additionally, edema of the paw induced by injection of 1% carrageenan was used to evaluate the anti-edema activity of A. polystachya. Oral doses of 100 and 200 mg/kg of crude extract of A. polystachya (CEAp) significantly reduced the sensibility to painful stimuli induced by the application of pressure in the tail comparable with an analgesic effect (p < 0.05) in a non-dose dependent manner. Additionally, the number of abdominal contortions was significantly reduced in comparison with the control group and with similar strength to the group treated with Indomethacin 10.0 mg/kg. Similarly, in the model of pain induced by thermal stimulation, it was observed that the groups treated with CEAp presented statistically significant analgesic activity in comparison with the control group and with a similar intensity to the group treated with morphine 6.0 mg/kg. Finally, a statistically significant reduction of edema induced by 1% carrageenan was observed with oral administration of 100 mg/kg of CEAp in comparison to the positive control of edema in a manner similar to the group treated with Indomethacin 10 mg/kg. Based on these results, it was concluded that the CEAp possesses the capacity to increase pain threshold in three pre-clinical models of pain induced (mechanical pressure, chemically and thermally) in mice, compatible with an analgesic effect. Also, CEAp demonstrated antiedematous capacity in carrageenan-induced paw edema in mice, concordant with anti-inflammatory effect using the plethysmography method. These pharmacological effects are potentially due to the presence of verbascoside in CEAp. Additionally, these experimental results are correlated with the popular use of CEAp and present a variety of opportunities for pharmaceutical research such as the development of innovative phytopharmaceuticals.

Research paper thumbnail of Effect of Prosopis ruscifolia on incretin and insulin secretion in alloxan-induced hyperglycemic rats

Vitae

Background: Diabetes mellitus treatment is based on oral hypoglycemic agents or insulin. Medicina... more Background: Diabetes mellitus treatment is based on oral hypoglycemic agents or insulin. Medicinal plants constitute an option, and the leaves of Prosopis ruscifolia (Pr) were shown to be effective in reducing glycemia in hyperglycemic animals. Objective: In this paper, we report the effect of P. rusciofolia (Pr) on insulin and incretin secretion in alloxan-induced hyperglycemic rats. Methodology: The effective dose was selected, and four groups (n=10) of Wistar rats were used. Two groups with normal glycemia received water or Pr (75 mg/Kg, per os, p.o.), and two groups with hyperglycemia induced by alloxan (intraperitoneal, ip), received water or Pr (75 mg/Kg, p.o.) for 2 weeks. Oral glucose tolerance test, and incretin and insulin levels were measured at the end of the experimental period. Results: The results showed that extract promotes better tolerance to oral glucose overload, in addition to a statistically significant (p<0.001) increase in blood levels of incretin and insu...

Research paper thumbnail of Diuretic and Hypertensive-Attenuating Effect of Crude Root Extract and Saponins from Solanum Sisymbriifolium Lam., in L-Name-Induced Hypertension in Rats

Research paper thumbnail of Pharmacological evaluation of Prosopis ruscifolia extract on lipid profile in hyperglycemic and hyperlipidemic mice

Journal of Applied Biology & Biotechnology

Diabetes is a serious chronic pathology, with long-term effects including damage to blood vessels... more Diabetes is a serious chronic pathology, with long-term effects including damage to blood vessels or diabetic dyslipidemia. Diabetic dyslipidemia is characterized by increasing concentrations of low-density triglycerides and lipoproteins and a decrease in high-density lipoproteins HDL-cholesterol (HDL-c). This study aimed to evaluate the effect of Prosopis ruscifolia on lipid profile in albino Swiss mice with hyperglycemia and hyperlipidemia. Hyperglycemia was induced by alloxan and the animals were orally treated with Pr (50, 100, and 200 mg/kg) for 45 days. Hyperlipidemia was induced with tyloxapol and the animals were treated with Pr (50, 100, and 200 mg/kg). In hyperglycemic animals treated with 100 mg/kg, there was a decrease in the concentration of cholesterol, a decrease in the concentration of triglycerides, and an increase in HDL-c at the end of treatment compared to untreated hyperglycemic animals. In mice with hyperlipidemia treated with 50 and 100 mg/kg of Pr, serum cholesterol and triglyceride concentration were reduced. HDL-c increased in animals treated with Pr 50, 100, and 200 mg/kg compared to untreated animals. It was observed that the administration of P. ruscifolia in hyperglycemic and hyperlipidemic animals had a favorable effect on the lipid profile.

Research paper thumbnail of Efectos adversos asociados a las vacunas COVID-19 en funcionarios y estudiantes de la Facultad de Ciencias Químicas de la UNA, año 2021

Revista de la Sociedad científica del Paraguay, May 31, 2023

Artículo publicado en acceso abierto bajo Licencia Creative Commons. Efectos adversos asociados a... more Artículo publicado en acceso abierto bajo Licencia Creative Commons. Efectos adversos asociados a las vacunas COVID-19 en funcionarios y estudiantes de la Facultad de Ciencias Químicas de la UNA, año 2021 Adverse effects associated with COVID-19 vaccines in officials and students of the Faculty of Chemical Sciences of UNA, year 2021

Research paper thumbnail of In Vivo Antidepressant-Like Effect Assessment of Two Aloysia Species in Mice and LCMS Chemical Characterization of Ethanol Extract

Molecules

Medicinal plants belonging to the Verbenaceae family demonstrated antidepressant effects in precl... more Medicinal plants belonging to the Verbenaceae family demonstrated antidepressant effects in preclinical studies. Depression is one of the largest contributors to the global health burden of all countries. Plants from the Aloysia genus are traditionally used for affective disorders, and some of them have proven anxiolytic and antidepressant activity. The aim of this work was to evaluate the antidepressant effect of the ethanolic extract of Aloysia gratissima var. gratissima (Agg) and Aloysia virgata var. platyphylla (Avp) in mice. A tail suspension test (TST) and forced swimming test (FST) were conducted after three doses in a period of 24 h and after 7 days of treatment. Imipramine was used as an antidepressant drug. The main results demonstrated that Agg extract reduced the immobility time in mice treated orally for 7 consecutive days when compared to the control group (reduced by about 77%, imipramine 70%). Animals treated with three doses of Avp in a 24-h period had reduced immob...

Research paper thumbnail of The antihypertensive and diuretic effect of crude root extract and saponins from Solanum sisymbriifolium Lam., in L-NAME-induced hypertension in rats

Journal of Ethnopharmacology

Research paper thumbnail of Determinación de parámetros bioquímicos de animales del Bioterio de la Facultad de Ciencias Químicas de la Universidad Nacional de Asunción

Revista de la Sociedad científica del Paraguay, May 31, 2023

Artículo publicado en acceso abierto bajo Licencia Creative Commons.

Research paper thumbnail of Attenuation of pain and inflammation induced in mice treated orally with crude extract of Aloysia polystachya (Griseb.) Moldenke (Verbenaceae)

Journal of Applied Pharmaceutical Science

The objective of this work was to determine the analgesic and anti-inflammatory activity of Aloys... more The objective of this work was to determine the analgesic and anti-inflammatory activity of Aloysia polystachya (Griseb.) Moldenke (Verbenaceae) in experimental models of acute pain and inflammation in mice. Methods of pain induced by caudal pressure (Randall-Selitto), chemical stimulation (acetic acid or the writhing test), and thermal stimulation (hot plate) were used to study analgesic effects. Additionally, edema of the paw induced by injection of 1% carrageenan was used to evaluate the anti-edema activity of A. polystachya. Oral doses of 100 and 200 mg/kg of crude extract of A. polystachya (CEAp) significantly reduced the sensibility to painful stimuli induced by the application of pressure in the tail comparable with an analgesic effect (p < 0.05) in a non-dose dependent manner. Additionally, the number of abdominal contortions was significantly reduced in comparison with the control group and with similar strength to the group treated with Indomethacin 10.0 mg/kg. Similarly, in the model of pain induced by thermal stimulation, it was observed that the groups treated with CEAp presented statistically significant analgesic activity in comparison with the control group and with a similar intensity to the group treated with morphine 6.0 mg/kg. Finally, a statistically significant reduction of edema induced by 1% carrageenan was observed with oral administration of 100 mg/kg of CEAp in comparison to the positive control of edema in a manner similar to the group treated with Indomethacin 10 mg/kg. Based on these results, it was concluded that the CEAp possesses the capacity to increase pain threshold in three pre-clinical models of pain induced (mechanical pressure, chemically and thermally) in mice, compatible with an analgesic effect. Also, CEAp demonstrated antiedematous capacity in carrageenan-induced paw edema in mice, concordant with anti-inflammatory effect using the plethysmography method. These pharmacological effects are potentially due to the presence of verbascoside in CEAp. Additionally, these experimental results are correlated with the popular use of CEAp and present a variety of opportunities for pharmaceutical research such as the development of innovative phytopharmaceuticals.

Research paper thumbnail of Effect of Prosopis ruscifolia on incretin and insulin secretion in alloxan-induced hyperglycemic rats

Vitae

Background: Diabetes mellitus treatment is based on oral hypoglycemic agents or insulin. Medicina... more Background: Diabetes mellitus treatment is based on oral hypoglycemic agents or insulin. Medicinal plants constitute an option, and the leaves of Prosopis ruscifolia (Pr) were shown to be effective in reducing glycemia in hyperglycemic animals. Objective: In this paper, we report the effect of P. rusciofolia (Pr) on insulin and incretin secretion in alloxan-induced hyperglycemic rats. Methodology: The effective dose was selected, and four groups (n=10) of Wistar rats were used. Two groups with normal glycemia received water or Pr (75 mg/Kg, per os, p.o.), and two groups with hyperglycemia induced by alloxan (intraperitoneal, ip), received water or Pr (75 mg/Kg, p.o.) for 2 weeks. Oral glucose tolerance test, and incretin and insulin levels were measured at the end of the experimental period. Results: The results showed that extract promotes better tolerance to oral glucose overload, in addition to a statistically significant (p<0.001) increase in blood levels of incretin and insu...

Research paper thumbnail of Diuretic and Hypertensive-Attenuating Effect of Crude Root Extract and Saponins from Solanum Sisymbriifolium Lam., in L-Name-Induced Hypertension in Rats

Research paper thumbnail of Pharmacological evaluation of Prosopis ruscifolia extract on lipid profile in hyperglycemic and hyperlipidemic mice

Journal of Applied Biology & Biotechnology

Diabetes is a serious chronic pathology, with long-term effects including damage to blood vessels... more Diabetes is a serious chronic pathology, with long-term effects including damage to blood vessels or diabetic dyslipidemia. Diabetic dyslipidemia is characterized by increasing concentrations of low-density triglycerides and lipoproteins and a decrease in high-density lipoproteins HDL-cholesterol (HDL-c). This study aimed to evaluate the effect of Prosopis ruscifolia on lipid profile in albino Swiss mice with hyperglycemia and hyperlipidemia. Hyperglycemia was induced by alloxan and the animals were orally treated with Pr (50, 100, and 200 mg/kg) for 45 days. Hyperlipidemia was induced with tyloxapol and the animals were treated with Pr (50, 100, and 200 mg/kg). In hyperglycemic animals treated with 100 mg/kg, there was a decrease in the concentration of cholesterol, a decrease in the concentration of triglycerides, and an increase in HDL-c at the end of treatment compared to untreated hyperglycemic animals. In mice with hyperlipidemia treated with 50 and 100 mg/kg of Pr, serum cholesterol and triglyceride concentration were reduced. HDL-c increased in animals treated with Pr 50, 100, and 200 mg/kg compared to untreated animals. It was observed that the administration of P. ruscifolia in hyperglycemic and hyperlipidemic animals had a favorable effect on the lipid profile.