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Papers by YAYA RUKAYADI / FOOD

Biodiversitas, May 27, 2023

Psidium guajava L. is well known as guava and has been used traditionally as a medicinal plant fo... more Psidium guajava L. is well known as guava and has been used traditionally as a medicinal plant for a variety of ailments all over the world, especially in infusions and decoctions for oral and topical use. Every part of this plant has been used for treating stomachache and diarrhea in many countries. Other reported uses include dermatitis, sores, epilepsy, and wounds. Medicinal plants have an important role in complementary and alternative medicine because they produce various natural compounds with high therapeutic properties. Many researchers report that the major chemical composition of this plant includes tannins, phenols, flavonoids, saponins, carbohydrates, alkaloids, sterols, terpenoids, and phenolic compounds. The aim of this study is to identify the volatile and non-volatile compounds in ethanolic P. guajava leaves extract. This study aimed to identify phytochemical compounds in P. guajava leaves extract. The main volatile compounds identified using GC-MS were pyrogallol, α-copaene, caryophyllene, aromadendrene, α-humulene, alloaromadendrene, γ-muurolene, β-selinene, α-selinene, αmuurolene, β-bisabolene, β-bisabolene, (-) globulol, caryophyllene oxide, α-muurolol, and epiglobulol. Based on LC-MS analysis, a number of non-volatile compounds such as flavonoids; apigenin 7-Ο-β-D-glucuronopyranoside, quercetin, luteolin 7-O-glucuronide, naringenin 4′-O-glucopyranoside, and myrecitin along with phenolic derivatives such as quinic acid, catechin, and epigallocatechin (4β,8)-gallocatechin were tentatively identified.

Chemistry & Biodiversity, 2011

The cytotoxicity of the methanol extract of Origanum syriacum against human cerivical adenocarcin... more The cytotoxicity of the methanol extract of Origanum syriacum against human cerivical adenocarcinoma, Hela cells, was evaluated by the MTT assay. From the crude extract, a new flavone glycoside, acacetin-7-O-[2-O-α-L-rhamnopyranosyl-6-O-β-D-glucopyranosyl]-β-D-glucopyranoside (1), together with the ten known flavonoids luteolin (2), apigenin (3), luteolin-6-C-glucoside (4), luteolin-3-methylether-6-C-glucoside (5), luteolin-7,4-dimethyether-6-C-glucoside (6), apigenin-7methylether-6-C-glucoside (7), apigenin-7-O-glucoside (8), diosmetin-7-O-glucoside (9), acacetin-7-O-glucoside (10) and acacetin-7-O-rutinoside (11), was isolated from the methanol extract of the aerial parts of Origanum syriacum. The structure elucidation of the isolated compounds was performed by spectroscopic and chromatographic methods.

International Journal of Molecular Sciences, 2016

The use of nanoparticle delivery systems to enhance intracellular penetration of antibiotics and ... more The use of nanoparticle delivery systems to enhance intracellular penetration of antibiotics and their retention time is becoming popular. The challenge, however, is that the interaction of nanoparticles with biological systems at the cellular level must be established prior to biomedical applications. Ciprofloxacin-cockle shells-derived calcium carbonate (aragonite) nanoparticles (C-CSCCAN) were developed and characterized. Antibacterial activity was determined using a modified disc diffusion protocol on Salmonella Typhimurium (S. Typhimurium). Biocompatibilittes with macrophage were evaluated using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 5-Bromo-2 1 -deoxyuridine (BrdU) assays. Transcriptional regulation of interleukin 1 beta (IL-1β) was determined using reverse transcriptase-polymerase chain reaction (RT-PCR). C-CSCCAN were spherical in shape, with particle sizes ranging from 11.93 to 22.12 nm. Encapsulation efficiency (EE) and loading content (LC) were 99.5% and 5.9%, respectively, with negative ζ potential. X-ray diffraction patterns revealed strong crystallizations and purity in the formulations. The mean diameter of inhibition zone was 18.6 ˘0.5 mm, which was better than ciprofloxacin alone (11.7 ˘0.9 mm). Study of biocompatability established the cytocompatability of the delivery system without upregulation of IL-1β. The results indicated that ciprofloxacin-nanoparticles enhanced the antibacterial efficacy of the antibiotic, and could act as a suitable delivery system against intracellular infections.

Fatimah Alqdeeri, 2019

Piper cubeba L. is the berry of a shrub that is indigenous to Java, Southern Borneo, Sumatra, and... more Piper cubeba L. is the berry of a shrub that is indigenous to Java, Southern Borneo, Sumatra, and other islands in the Indian Ocean. The plant is usually used in folk traditional medicine and is an important ingredient in cooking. The purpose of this study was to isolate and purify the bioactive compounds from P. cubeba L. fractions. In addition, the isolated compounds were tested for their antibacterial and antispore activities against vegetative cells and spores of Bacillus cereus ATCC33019, B. subtilis ATCC6633, B. pumilus ATCC14884, and B. megaterium ATCC14581. The phytochemical investigation of the DCM fraction yielded two known compounds: β-asarone (1), and asaronaldehyde (2) were successfully isolated and identified from the methanol extract and its fractions of P. cubeba L. Results showed that exposing the vegetative cells of Bacillus sp. to isolated compounds resulted in an inhibition zone with a large diameter ranging between 7.21 to 9.61 mm. The range of the minimum inhibitory concentration (MIC) was between 63.0 to 125.0 µg/mL and had minimum bactericidal concentration (MBC) at 250.0 to 500.0 µg/mL against Bacillus sp. Isolated compounds at a concentration of 0.05% inactivated more than 3-Log 10 (90.99%) of the spores of Bacillus sp. after an incubation period of four hours, and all the spores were killed at a concentration of 0.1%. The structures were recognizably elucidated based on 1D and 2D-NMR analyses (1H, 13C, COSY, HSQC, and HMBC) and mass spectrometry data. Compounds 1, and 2 were isolated for the first time from this plant. In conclusion, the two compounds show a promising potential of antibacterial and sporicidal activities against Bacillus sp. and thus can be developed as an anti-Bacillus agent.

Biodiversitas, May 27, 2023

Psidium guajava L. is well known as guava and has been used traditionally as a medicinal plant fo... more Psidium guajava L. is well known as guava and has been used traditionally as a medicinal plant for a variety of ailments all over the world, especially in infusions and decoctions for oral and topical use. Every part of this plant has been used for treating stomachache and diarrhea in many countries. Other reported uses include dermatitis, sores, epilepsy, and wounds. Medicinal plants have an important role in complementary and alternative medicine because they produce various natural compounds with high therapeutic properties. Many researchers report that the major chemical composition of this plant includes tannins, phenols, flavonoids, saponins, carbohydrates, alkaloids, sterols, terpenoids, and phenolic compounds. The aim of this study is to identify the volatile and non-volatile compounds in ethanolic P. guajava leaves extract. This study aimed to identify phytochemical compounds in P. guajava leaves extract. The main volatile compounds identified using GC-MS were pyrogallol, α-copaene, caryophyllene, aromadendrene, α-humulene, alloaromadendrene, γ-muurolene, β-selinene, α-selinene, αmuurolene, β-bisabolene, β-bisabolene, (-) globulol, caryophyllene oxide, α-muurolol, and epiglobulol. Based on LC-MS analysis, a number of non-volatile compounds such as flavonoids; apigenin 7-Ο-β-D-glucuronopyranoside, quercetin, luteolin 7-O-glucuronide, naringenin 4′-O-glucopyranoside, and myrecitin along with phenolic derivatives such as quinic acid, catechin, and epigallocatechin (4β,8)-gallocatechin were tentatively identified.

Chemistry & Biodiversity, 2011

The cytotoxicity of the methanol extract of Origanum syriacum against human cerivical adenocarcin... more The cytotoxicity of the methanol extract of Origanum syriacum against human cerivical adenocarcinoma, Hela cells, was evaluated by the MTT assay. From the crude extract, a new flavone glycoside, acacetin-7-O-[2-O-α-L-rhamnopyranosyl-6-O-β-D-glucopyranosyl]-β-D-glucopyranoside (1), together with the ten known flavonoids luteolin (2), apigenin (3), luteolin-6-C-glucoside (4), luteolin-3-methylether-6-C-glucoside (5), luteolin-7,4-dimethyether-6-C-glucoside (6), apigenin-7methylether-6-C-glucoside (7), apigenin-7-O-glucoside (8), diosmetin-7-O-glucoside (9), acacetin-7-O-glucoside (10) and acacetin-7-O-rutinoside (11), was isolated from the methanol extract of the aerial parts of Origanum syriacum. The structure elucidation of the isolated compounds was performed by spectroscopic and chromatographic methods.

International Journal of Molecular Sciences, 2016

The use of nanoparticle delivery systems to enhance intracellular penetration of antibiotics and ... more The use of nanoparticle delivery systems to enhance intracellular penetration of antibiotics and their retention time is becoming popular. The challenge, however, is that the interaction of nanoparticles with biological systems at the cellular level must be established prior to biomedical applications. Ciprofloxacin-cockle shells-derived calcium carbonate (aragonite) nanoparticles (C-CSCCAN) were developed and characterized. Antibacterial activity was determined using a modified disc diffusion protocol on Salmonella Typhimurium (S. Typhimurium). Biocompatibilittes with macrophage were evaluated using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 5-Bromo-2 1 -deoxyuridine (BrdU) assays. Transcriptional regulation of interleukin 1 beta (IL-1β) was determined using reverse transcriptase-polymerase chain reaction (RT-PCR). C-CSCCAN were spherical in shape, with particle sizes ranging from 11.93 to 22.12 nm. Encapsulation efficiency (EE) and loading content (LC) were 99.5% and 5.9%, respectively, with negative ζ potential. X-ray diffraction patterns revealed strong crystallizations and purity in the formulations. The mean diameter of inhibition zone was 18.6 ˘0.5 mm, which was better than ciprofloxacin alone (11.7 ˘0.9 mm). Study of biocompatability established the cytocompatability of the delivery system without upregulation of IL-1β. The results indicated that ciprofloxacin-nanoparticles enhanced the antibacterial efficacy of the antibiotic, and could act as a suitable delivery system against intracellular infections.

Fatimah Alqdeeri, 2019

Piper cubeba L. is the berry of a shrub that is indigenous to Java, Southern Borneo, Sumatra, and... more Piper cubeba L. is the berry of a shrub that is indigenous to Java, Southern Borneo, Sumatra, and other islands in the Indian Ocean. The plant is usually used in folk traditional medicine and is an important ingredient in cooking. The purpose of this study was to isolate and purify the bioactive compounds from P. cubeba L. fractions. In addition, the isolated compounds were tested for their antibacterial and antispore activities against vegetative cells and spores of Bacillus cereus ATCC33019, B. subtilis ATCC6633, B. pumilus ATCC14884, and B. megaterium ATCC14581. The phytochemical investigation of the DCM fraction yielded two known compounds: β-asarone (1), and asaronaldehyde (2) were successfully isolated and identified from the methanol extract and its fractions of P. cubeba L. Results showed that exposing the vegetative cells of Bacillus sp. to isolated compounds resulted in an inhibition zone with a large diameter ranging between 7.21 to 9.61 mm. The range of the minimum inhibitory concentration (MIC) was between 63.0 to 125.0 µg/mL and had minimum bactericidal concentration (MBC) at 250.0 to 500.0 µg/mL against Bacillus sp. Isolated compounds at a concentration of 0.05% inactivated more than 3-Log 10 (90.99%) of the spores of Bacillus sp. after an incubation period of four hours, and all the spores were killed at a concentration of 0.1%. The structures were recognizably elucidated based on 1D and 2D-NMR analyses (1H, 13C, COSY, HSQC, and HMBC) and mass spectrometry data. Compounds 1, and 2 were isolated for the first time from this plant. In conclusion, the two compounds show a promising potential of antibacterial and sporicidal activities against Bacillus sp. and thus can be developed as an anti-Bacillus agent.