Yisehak Tsegaye - Academia.edu (original) (raw)
Papers by Yisehak Tsegaye
Research Square (Research Square), Oct 16, 2023
Diversifying the use of climate-smart crops such as Sweet sorghum has the potential to solve inte... more Diversifying the use of climate-smart crops such as Sweet sorghum has the potential to solve integrated food, bioenergy, feed, and land management problems. The study purposed to quantify the sugar content of Nech Tinkish (v1) and Hawaye (v2) indigenous sweet sorghum varieties and investigate the interaction effect of fermentation parameters to determine their capacity for ethanol production. Sweet sorghum varieties were analyzed to determine their difference in oBrix content by extracting their juices. The juice was clari ed using milk lime. Its total soluble sugars, total carbohydrates, and reducing sugars were determined using a digital Refractometer, phenol sulfuric acid, and 3, 5-Dinitro salicylic acid, respectively. A completely Randomized Factorial was employed to evaluate ethanol production capacity, and the ethanol content was estimated using a potassium dichromate solution. The oBrix results revealed that v2 had a higher sugar concentration than v1. Additionally, the estimated carbohydrate content of the juice ranged from 37.402 g/L to 157.641 g/L. The estimated reducing sugar also varied from 4.644 g/L to 33.412 g/L. Therefore, the estimated reducing sugar showed the hydrolysis of Sweet sorghum juice by invertase and sulfuric acid produced more fermentable sugars. Fermentation at 30 o C with pH 4.5 incubated for four days yields the highest ethanol, and v2 yields higher (15.31%) ethanol, compared to v1 produced 15.31%. This study showed a basis for the existence of two sugar-rich climate smart sweet sorghum varieties with an extraordinary amount of sugar used as a source of biofuel and food simultaneously in a single plot of land.
Research Square (Research Square), Jun 22, 2023
International Microbiology
![Research paper thumbnail of Production of Bioethanol from Sweet Sorghum [Sorghum Bicolor L.] Juice Using Yeast Isolated from Fermented Sweet Sorghum Juice](https://attachments.academia-assets.com/117744967/thumbnails/1.jpg)
](Background Nonrenewable energies have been used as the major sources of energy in the world so fa... more Background Nonrenewable energies have been used as the major sources of energy in the world so far. However, they are not sustainable and environmentally friendly and there is an urgent need to search for alternative renewable sustainable energy sources. Sweet sorghum is a sugar rich plant that can be used as an important alternative source of bioenergy that is renewable with a low contribution to global warming without computing food security. This study aimed to investigate the potential of sweet sorghum juice for bioethanol production using yeast isolated from fermented sweet sorghum juice. Methods Sweet sorghum juice oBrix was determined using a digital refractometer.Yeasts isolated from fermented sweet sorghum juice were characterized using different biochemical tests. Potent yeast was used in the fermentation of sweet sorghum juice which was carried out at different fermentation parameters, mainly pH, temperature, and incubation period, using a completely randomized factorial ...
<p>Incidence proportion of human rabies per 100,000 population in the study districts from ... more <p>Incidence proportion of human rabies per 100,000 population in the study districts from 2012–2016.</p
<p>District wise retrospective data of rabies among different species of animals (2012–2016... more <p>District wise retrospective data of rabies among different species of animals (2012–2016).</p
<p>Correlation matrix on rabies cases in humans and animals.</p
<p>Summary of the human population of the study districts.</p
<p>Overall incidence proportion of major zoonotic diseases per 100,000 populations in the s... more <p>Overall incidence proportion of major zoonotic diseases per 100,000 populations in the study districts from 2012–2016.</p
Ethiopian Journal of Health Development, 2018
Background: According to the recommendation made by World Health organization, vaccinating 70% of... more Background: According to the recommendation made by World Health organization, vaccinating 70% of the dog population helps to control rabies and prevent rabies virus in human population. However, the exponential increase in the population of free roaming dogs is a serious challenge to this strategy in Eastern African countries including Ethiopia and Kenya. Understanding the dynamics of free roaming dog populations is, thus, a step to be taken prior to designing effective rabies prevention and control strategy in these countries. Objectives: The present study was designed to determine the number of free roaming dogs in selected settings in Ethiopia and Kenya, and describe the level of community knowledge, attitude and practice (KAP) on rabies incidence/human exposures. The study also described the socio-cultural value of dog keeping in the areas considered in the study. Methodology: Counting free roaming dogs were a major means of collecting data in both Ethiopia and Kenya. Dog count was made using the markup capture approach. Other than counting, questionnaire was used to obtain data for the study. Three-hundred and ninety-eight copies of questionnaires were administered to the study participants in Ethiopia, while the number of respondents to the questionnaire in Kenya was 351. In addition, a five-year retrospective data on dog/animal bite cases were collected from selected health facilities of the study sites. Results: A total of 2991 and 386 free roaming dogs were counted in Ethiopia and Kenya, respectively. A five-year retrospective data showed cases of 1524 (in Mekelle) and 429 (Assela) individuals who were bitten/infected by rabies-suspected animals. Evidence obtained from the health facilities in Mekelle and Assela showed the bitten/infected individuals took PEP within the specified period. In Kenya, a total of 3441 and 4997 animal bite cases were reported from 2010-2014 in Kisumu and Siaya, respectively. The number of animal bite cases may signify the economic burden incurred (cost of PEP and other related costs), public health impact and social value of the disease. The questionnaire data also indicated the existing dog management practices, awareness of the community about rabies and its zoonotic importance, the first line of action taken at home for individuals bitten by rabies suspected animal, awareness of the community on dog vaccination, importance of free roaming dogs and their management. Conclusion: The significant proportion of free roaming dogs and number of animal bite cases calls for an integrated action between human and veterinary professionals to control the number of free roaming dog population, initiate awareness creation programs in the community and increase the vaccination of owned dogs there by to control and prevent rabies.
Study on mange mite of camel in Raya-Azebo district, northern Ethiopia
PLOS ONE, 2021
Neglected tropical zoonotic diseases (NTZDs) continue to have a major effect on the health of hum... more Neglected tropical zoonotic diseases (NTZDs) continue to have a major effect on the health of humans and animals. In this study, a one health approach was used to prioritize and rank neglected tropical zoonotic diseases at the regional and zonal levels in Tigray National Regional State, Ethiopia. For prioritization of NTZDs a cross-sectional study through a structured questionnaire was administered to 313 health experts from human and animal health sectors. In addition, focus group discussions (FGD) were held with purposively selected key informants. Descriptive, and Multivariable analysis was applied to report the results and a ranked list of diseases was developed at the zonal and regional level. In the region, 8 of the 12 World Health Organization listed NTZDs were considered major diseases including anthrax, brucellosis, bovine tuberculosis, taeniasis, leishmaniasis, rabies, schistosomiasis, and soil-transmitted helminths. Considering the zoonotic and socioeconomic importance of...
Carbon Management, 2021
Abstract Though livestock are one of the major contributors to climate change through emission of... more Abstract Though livestock are one of the major contributors to climate change through emission of greenhouse gases (GHG), the intensity of emission and the mitigation potential from this sector is not clearly known in the study regions. Therefore, the present study was designed to estimate the emission of GHGs from the livestock sector over the time period (2003/04–2017/18) and evaluate the mitigation potential using the global livestock environment assessment model (GLEAM). Both primary and secondary data sources were used to obtain data on livestock population, herd parameters, feeding practices, and manure management. The study revealed an increasing trend of GHG from 2003/04 to 2017/18, where the annual emission increased with an average 3.8 Mt carbon dioxide equivalent (CO2-eq) for all species and 3.3 Mt CO2-eq for cattle alone at national level. Though there is some variation between regions, methane (CH4) was the major gas emitted from ruminant production followed by nitrous oxide (N2O), however the emission of N2O was higher (> 70%) in poultry production. The emission intensity from chicken egg and meat, 1.8–3.3 and 2.2–7.2 kg CO2-eq/kg of product, respectively, was much lower than the emission intensity of meat from small ruminants (16.4–51.5 kg CO2-eq/kg of product) and cattle (34.2–148.2 kg CO2-eq/kg of product). Implementing the integrated mitigation approach has much pronounced reduction effect on GHG emissions as well as increasing the productivity of the livestock sector. Unless mitigation measures are taken, the sector’s emission will increase over time. Thus, federal and regional governments, and partner organizations working in the sector need to give more attention and implement the initiatives identified in the Climate Resilient Green Economy (CRGE) strategy and Food and Agriculture Organization of the United Nations (FAO) recommendations to mitigate GHG emissions from the livestock sector.
Zoonoses and Public Health, 2021
Neglected tropical zoonotic diseases (NTZDs) continue to affect the health and livelihoods of hum... more Neglected tropical zoonotic diseases (NTZDs) continue to affect the health and livelihoods of humans particularly the poor and marginalized populations in developing countries. Mapping the distribution and burden of these diseases will support making an informed decision. A retrospective study was conducted to map the spatial distribution and analyse trend of NTZDs in Tigray region, Northern Ethiopia. A health management information system (HMIS) data between 2012/13 and 2016/17 was obtained from Tigray National Regional State Health Bureau. The Quantum‐GIS software was used to map the spatial distribution and burden of selected NTZDs at zonal level. Only four of the NTZDs namely rabies, tuberculosis (TB), schistosomiasis and visceral leishmaniasis (VL) from the records of HMIS data were considered. A high incidence rate (IR) at least for one of the NTZDs was reported in males above 15 years old. In the 5‐year period, of the 60,099 reported NTZD cases, 30.3% (18,220), 26.6% (16,005)...
Nanomedicine: Nanotechnology, Biology and Medicine, 2019
Journal of immunoassay & immunochemistry, 2016
The PCR amplified HA1 fragment of H5N1 (H5HA1) avian influenza virus (AIV) haemagglutinin gene wa... more The PCR amplified HA1 fragment of H5N1 (H5HA1) avian influenza virus (AIV) haemagglutinin gene was cloned into pET28a (+) expression vector and expressed in Rosetta Blue (DE3) pLysS cells. The recombinant H5HA1 (rH5HA1) protein purified by passive gel elution after SDS-PAGE of the inclusion bodies reacted specifically with H5N1 serum in Western blot analysis. A subtype specific indirect enzyme linked immunosorbent assay (iELISA) using the rH5HA1 protein as the coating antigen was developed for detecting antibodies to H5 subtype of AIV. The assay had 89.04% sensitivity and 95.95% specificity when compared with haemagglutination inhibition test. The Kappa value of 0.842 indicated a perfect agreement between the tests. The iELISA developed can be used for serosurveillance of avian influenza in chickens.
Onderstepoort J Vet Res, 2015
A cross-sectional study was conducted from November 2011 to April 2012 on a total of 384 pigs fro... more A cross-sectional study was conducted from November 2011 to April 2012 on a total of 384 pigs from two privately owned intensive farms in Bishoftu, Ethiopia. The objectives of the study were to identify and determine the prevalence of common parasites of pigs. For the determination of gastrointestinal (GIT) parasites, faecal samples were collected from the study animals and subjected to standard parasitological examination techniques. Physical examination was conducted for the presence of skin parasitic lesions and skin scrapings were collected to determine prevalence of ectoparasites. The overall prevalence of GIT parasites in the pigs was 25% (96/384). Examination of faecal samples revealed the ova or oocysts of four different gastrointestinal parasites, namely Coccidia (12%), Strongyles (5.2%), Ascaris suum (4.9%) and Trichuris suis (2.9%). Mixed infection by at least two parasite species was observed in 3.65% (14/384) of the pigs. The only ectoparasite species identified was Sar...
Journal of Parasitology and Vector Biology, 2014
Veterinary research forum : an international quarterly journal, 2014
A cross-sectional study was carried out to determine the prevalence and species of camel mange mi... more A cross-sectional study was carried out to determine the prevalence and species of camel mange mite infestation in Raya-Azebo district, Northern part of Ethiopia. Accordingly, Three hundred and eighty-four camels were examined and mange mite infestation was detected on 64 of camels. Only Sarcoptes scabiei var. cameli was identified as the only mite species in all skin scraping samples collected from the suspected mange mite lesions. There was significant difference in the prevalence of mange mite infestation between male and female camels (p < 0.05) but no significance difference was observed among the age groups and body condition score of camels (p…
Research Square (Research Square), Oct 16, 2023
Diversifying the use of climate-smart crops such as Sweet sorghum has the potential to solve inte... more Diversifying the use of climate-smart crops such as Sweet sorghum has the potential to solve integrated food, bioenergy, feed, and land management problems. The study purposed to quantify the sugar content of Nech Tinkish (v1) and Hawaye (v2) indigenous sweet sorghum varieties and investigate the interaction effect of fermentation parameters to determine their capacity for ethanol production. Sweet sorghum varieties were analyzed to determine their difference in oBrix content by extracting their juices. The juice was clari ed using milk lime. Its total soluble sugars, total carbohydrates, and reducing sugars were determined using a digital Refractometer, phenol sulfuric acid, and 3, 5-Dinitro salicylic acid, respectively. A completely Randomized Factorial was employed to evaluate ethanol production capacity, and the ethanol content was estimated using a potassium dichromate solution. The oBrix results revealed that v2 had a higher sugar concentration than v1. Additionally, the estimated carbohydrate content of the juice ranged from 37.402 g/L to 157.641 g/L. The estimated reducing sugar also varied from 4.644 g/L to 33.412 g/L. Therefore, the estimated reducing sugar showed the hydrolysis of Sweet sorghum juice by invertase and sulfuric acid produced more fermentable sugars. Fermentation at 30 o C with pH 4.5 incubated for four days yields the highest ethanol, and v2 yields higher (15.31%) ethanol, compared to v1 produced 15.31%. This study showed a basis for the existence of two sugar-rich climate smart sweet sorghum varieties with an extraordinary amount of sugar used as a source of biofuel and food simultaneously in a single plot of land.
Research Square (Research Square), Jun 22, 2023
International Microbiology
Background Nonrenewable energies have been used as the major sources of energy in the world so fa... more Background Nonrenewable energies have been used as the major sources of energy in the world so far. However, they are not sustainable and environmentally friendly and there is an urgent need to search for alternative renewable sustainable energy sources. Sweet sorghum is a sugar rich plant that can be used as an important alternative source of bioenergy that is renewable with a low contribution to global warming without computing food security. This study aimed to investigate the potential of sweet sorghum juice for bioethanol production using yeast isolated from fermented sweet sorghum juice. Methods Sweet sorghum juice oBrix was determined using a digital refractometer.Yeasts isolated from fermented sweet sorghum juice were characterized using different biochemical tests. Potent yeast was used in the fermentation of sweet sorghum juice which was carried out at different fermentation parameters, mainly pH, temperature, and incubation period, using a completely randomized factorial ...
<p>Incidence proportion of human rabies per 100,000 population in the study districts from ... more <p>Incidence proportion of human rabies per 100,000 population in the study districts from 2012–2016.</p
<p>District wise retrospective data of rabies among different species of animals (2012–2016... more <p>District wise retrospective data of rabies among different species of animals (2012–2016).</p
<p>Correlation matrix on rabies cases in humans and animals.</p
<p>Summary of the human population of the study districts.</p
<p>Overall incidence proportion of major zoonotic diseases per 100,000 populations in the s... more <p>Overall incidence proportion of major zoonotic diseases per 100,000 populations in the study districts from 2012–2016.</p
Ethiopian Journal of Health Development, 2018
Background: According to the recommendation made by World Health organization, vaccinating 70% of... more Background: According to the recommendation made by World Health organization, vaccinating 70% of the dog population helps to control rabies and prevent rabies virus in human population. However, the exponential increase in the population of free roaming dogs is a serious challenge to this strategy in Eastern African countries including Ethiopia and Kenya. Understanding the dynamics of free roaming dog populations is, thus, a step to be taken prior to designing effective rabies prevention and control strategy in these countries. Objectives: The present study was designed to determine the number of free roaming dogs in selected settings in Ethiopia and Kenya, and describe the level of community knowledge, attitude and practice (KAP) on rabies incidence/human exposures. The study also described the socio-cultural value of dog keeping in the areas considered in the study. Methodology: Counting free roaming dogs were a major means of collecting data in both Ethiopia and Kenya. Dog count was made using the markup capture approach. Other than counting, questionnaire was used to obtain data for the study. Three-hundred and ninety-eight copies of questionnaires were administered to the study participants in Ethiopia, while the number of respondents to the questionnaire in Kenya was 351. In addition, a five-year retrospective data on dog/animal bite cases were collected from selected health facilities of the study sites. Results: A total of 2991 and 386 free roaming dogs were counted in Ethiopia and Kenya, respectively. A five-year retrospective data showed cases of 1524 (in Mekelle) and 429 (Assela) individuals who were bitten/infected by rabies-suspected animals. Evidence obtained from the health facilities in Mekelle and Assela showed the bitten/infected individuals took PEP within the specified period. In Kenya, a total of 3441 and 4997 animal bite cases were reported from 2010-2014 in Kisumu and Siaya, respectively. The number of animal bite cases may signify the economic burden incurred (cost of PEP and other related costs), public health impact and social value of the disease. The questionnaire data also indicated the existing dog management practices, awareness of the community about rabies and its zoonotic importance, the first line of action taken at home for individuals bitten by rabies suspected animal, awareness of the community on dog vaccination, importance of free roaming dogs and their management. Conclusion: The significant proportion of free roaming dogs and number of animal bite cases calls for an integrated action between human and veterinary professionals to control the number of free roaming dog population, initiate awareness creation programs in the community and increase the vaccination of owned dogs there by to control and prevent rabies.
Study on mange mite of camel in Raya-Azebo district, northern Ethiopia
PLOS ONE, 2021
Neglected tropical zoonotic diseases (NTZDs) continue to have a major effect on the health of hum... more Neglected tropical zoonotic diseases (NTZDs) continue to have a major effect on the health of humans and animals. In this study, a one health approach was used to prioritize and rank neglected tropical zoonotic diseases at the regional and zonal levels in Tigray National Regional State, Ethiopia. For prioritization of NTZDs a cross-sectional study through a structured questionnaire was administered to 313 health experts from human and animal health sectors. In addition, focus group discussions (FGD) were held with purposively selected key informants. Descriptive, and Multivariable analysis was applied to report the results and a ranked list of diseases was developed at the zonal and regional level. In the region, 8 of the 12 World Health Organization listed NTZDs were considered major diseases including anthrax, brucellosis, bovine tuberculosis, taeniasis, leishmaniasis, rabies, schistosomiasis, and soil-transmitted helminths. Considering the zoonotic and socioeconomic importance of...
Carbon Management, 2021
Abstract Though livestock are one of the major contributors to climate change through emission of... more Abstract Though livestock are one of the major contributors to climate change through emission of greenhouse gases (GHG), the intensity of emission and the mitigation potential from this sector is not clearly known in the study regions. Therefore, the present study was designed to estimate the emission of GHGs from the livestock sector over the time period (2003/04–2017/18) and evaluate the mitigation potential using the global livestock environment assessment model (GLEAM). Both primary and secondary data sources were used to obtain data on livestock population, herd parameters, feeding practices, and manure management. The study revealed an increasing trend of GHG from 2003/04 to 2017/18, where the annual emission increased with an average 3.8 Mt carbon dioxide equivalent (CO2-eq) for all species and 3.3 Mt CO2-eq for cattle alone at national level. Though there is some variation between regions, methane (CH4) was the major gas emitted from ruminant production followed by nitrous oxide (N2O), however the emission of N2O was higher (> 70%) in poultry production. The emission intensity from chicken egg and meat, 1.8–3.3 and 2.2–7.2 kg CO2-eq/kg of product, respectively, was much lower than the emission intensity of meat from small ruminants (16.4–51.5 kg CO2-eq/kg of product) and cattle (34.2–148.2 kg CO2-eq/kg of product). Implementing the integrated mitigation approach has much pronounced reduction effect on GHG emissions as well as increasing the productivity of the livestock sector. Unless mitigation measures are taken, the sector’s emission will increase over time. Thus, federal and regional governments, and partner organizations working in the sector need to give more attention and implement the initiatives identified in the Climate Resilient Green Economy (CRGE) strategy and Food and Agriculture Organization of the United Nations (FAO) recommendations to mitigate GHG emissions from the livestock sector.
Zoonoses and Public Health, 2021
Neglected tropical zoonotic diseases (NTZDs) continue to affect the health and livelihoods of hum... more Neglected tropical zoonotic diseases (NTZDs) continue to affect the health and livelihoods of humans particularly the poor and marginalized populations in developing countries. Mapping the distribution and burden of these diseases will support making an informed decision. A retrospective study was conducted to map the spatial distribution and analyse trend of NTZDs in Tigray region, Northern Ethiopia. A health management information system (HMIS) data between 2012/13 and 2016/17 was obtained from Tigray National Regional State Health Bureau. The Quantum‐GIS software was used to map the spatial distribution and burden of selected NTZDs at zonal level. Only four of the NTZDs namely rabies, tuberculosis (TB), schistosomiasis and visceral leishmaniasis (VL) from the records of HMIS data were considered. A high incidence rate (IR) at least for one of the NTZDs was reported in males above 15 years old. In the 5‐year period, of the 60,099 reported NTZD cases, 30.3% (18,220), 26.6% (16,005)...
Nanomedicine: Nanotechnology, Biology and Medicine, 2019
Journal of immunoassay & immunochemistry, 2016
The PCR amplified HA1 fragment of H5N1 (H5HA1) avian influenza virus (AIV) haemagglutinin gene wa... more The PCR amplified HA1 fragment of H5N1 (H5HA1) avian influenza virus (AIV) haemagglutinin gene was cloned into pET28a (+) expression vector and expressed in Rosetta Blue (DE3) pLysS cells. The recombinant H5HA1 (rH5HA1) protein purified by passive gel elution after SDS-PAGE of the inclusion bodies reacted specifically with H5N1 serum in Western blot analysis. A subtype specific indirect enzyme linked immunosorbent assay (iELISA) using the rH5HA1 protein as the coating antigen was developed for detecting antibodies to H5 subtype of AIV. The assay had 89.04% sensitivity and 95.95% specificity when compared with haemagglutination inhibition test. The Kappa value of 0.842 indicated a perfect agreement between the tests. The iELISA developed can be used for serosurveillance of avian influenza in chickens.
Onderstepoort J Vet Res, 2015
A cross-sectional study was conducted from November 2011 to April 2012 on a total of 384 pigs fro... more A cross-sectional study was conducted from November 2011 to April 2012 on a total of 384 pigs from two privately owned intensive farms in Bishoftu, Ethiopia. The objectives of the study were to identify and determine the prevalence of common parasites of pigs. For the determination of gastrointestinal (GIT) parasites, faecal samples were collected from the study animals and subjected to standard parasitological examination techniques. Physical examination was conducted for the presence of skin parasitic lesions and skin scrapings were collected to determine prevalence of ectoparasites. The overall prevalence of GIT parasites in the pigs was 25% (96/384). Examination of faecal samples revealed the ova or oocysts of four different gastrointestinal parasites, namely Coccidia (12%), Strongyles (5.2%), Ascaris suum (4.9%) and Trichuris suis (2.9%). Mixed infection by at least two parasite species was observed in 3.65% (14/384) of the pigs. The only ectoparasite species identified was Sar...
Journal of Parasitology and Vector Biology, 2014
Veterinary research forum : an international quarterly journal, 2014
A cross-sectional study was carried out to determine the prevalence and species of camel mange mi... more A cross-sectional study was carried out to determine the prevalence and species of camel mange mite infestation in Raya-Azebo district, Northern part of Ethiopia. Accordingly, Three hundred and eighty-four camels were examined and mange mite infestation was detected on 64 of camels. Only Sarcoptes scabiei var. cameli was identified as the only mite species in all skin scraping samples collected from the suspected mange mite lesions. There was significant difference in the prevalence of mange mite infestation between male and female camels (p < 0.05) but no significance difference was observed among the age groups and body condition score of camels (p…