Darlina Yusuf - Academia.edu (original) (raw)

Papers by Darlina Yusuf

Asian Pacific Journal of Cancer Prevention

Objective: The purpose of this study was to assess the immune response and malondialdehyde levels... more Objective: The purpose of this study was to assess the immune response and malondialdehyde levels in irradiated rats supplemented with Curcuma xanthorriza Roxb extract as a candidate for mitigating radiation exposure. Methods: Twenty-four male Wistar rats were grouped into eight treatment groups, then Curcuma xanthorrhiza Roxb extract was administered orally and irradiated at 6 Gy. Measurement of rats IL-6 and INF-γ was performed using a sandwich ELISA Kit, while the MDA concentration was quantified according to the method of Wills (1971). The statistical test is determined by one way ANOVA test. P-value <0.05 was considered statistically significant. Result: The concentration of IL-6 in all groups showed no statistically significant difference (P=0.18). There was an increase in the concentration of IL-6 in the group of rats irradiated with 6 Gy for 7 days and 14 days. Meanwhile, the INF-γ concentration also showed no significant results in all treatment groups (P=0.28). The average of MDA concentration showed a significant difference in the liver and spleen of irradiated rats at 6 Gy for 14 days compared to the control (0.044 nmol/mg vs 0.008 nmol/ mg, P=0.03 and 0.032 nmol/mg vs 0.014 nmol/mg, P=0.05, respectively). Conclusion: The administration of Curcuma xanthorriza Xorb extract was able to reduce MDA concentrations in the liver and spleen although not statistically significant. In addition, exposure to ionizing radiation at a dose of 6 Gy significantly increased lipid peroxidation in the liver and spleen by 5.5 times and 2.3 times, respectively.

Asian Pacific Journal of Cancer Prevention

Objective: The aim of this study was to assess the effect of radiation exposure, human 8-oxoguani... more Objective: The aim of this study was to assess the effect of radiation exposure, human 8-oxoguanine DNA N-glycosylase-1 (hOGG1) exon 7 genetic polymorphism and confounding factors on DNA damage response. Methods: Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and alkaline Comet assay method were applied to determine the hOGG1 genetic polymorphisms and DNA damage response. A total of 80 participants were enrolled in this study, consisting of 40 radiation-exposed workers as a case group and 40 non-radiation workers as a control group. Result: The genotypes frequencies for controls were Ser/Ser (35%), Ser/Cys (32.5%), and Cys/ Cys (32.5%), with frequencies of alleles being 326Ser (0.52) and 326Cys (0.48), whereas the genotypes frequencies for radiation-exposed workers (cases group) were Ser/Ser (17.5%), Ser/Cys (57.5%), and Cys/Cys (25%), with frequencies of alleles being 326Ser (0.46) and 326Cys (0.54). The results indicated that DNA damage response were not significantly higher in the exposed workers than in controls (22.55 ± 6.02 versus 21.72 ± 7.14; P=0.58). The time of exposure has a significantly negative correlation with comet tail length value among radiation workers. In addition, it was found that the DNA damage response was strongly associated with age and time of exposure with a decrease of 0.6 percent (P-value: 0.008) and 0.58 percent (P-value: 0.009), respectively. Whereas gender, smoking habit, and equivalent dose were not correlated with DNA damage. Conclusion: The single-nucleotide polymorphism of hOGG1 exon 7 (rs1052133) demonstrated no association with the extent of DNA damage in radiation-exposed workers.

Jurnal Forum Nuklir, 2014

EFEKTIFITAS KLOROKUIN TERHADAP PERTUMBUHAN Plasmodium falciparum RADlASI SECARA IN VITRO. Dalam u... more EFEKTIFITAS KLOROKUIN TERHADAP PERTUMBUHAN Plasmodium falciparum RADlASI SECARA IN VITRO. Dalam uji coba bahan vaksin malaria untuk mencegah relawan dari penyakit malaria maka digunakan chemoprophylaxis yang dikombinasikan dengan bahan vaksin yang akan diteliti. Klorokuin merupakan antimalaria yang umum digunakan sebagai chemoprophylaxis. Tujuan penelitian ini adalah untuk mengetahui efektivitas klorokuin terhadap pertumbuhan P. falciparum 3D7 radiasi. Pengujian Efektivitas klorokuin dilakukan dengan mengencerkan larutan stok 100 µg/ml sebesar 10-4, 10-5, 10-6, 10-7, 10-8 dan 10-9 . Masing-masing pengenceran dimasukkan ke dalam 40 µI suspensi sel darah merah terinfeksi parasit P. falciparum radiasi maupun yang tidak diradiasi dan diinkubasi pada 37°C. Jumlah parasit yang bertahan hidup dihitung setiap hari selama 7 hari melalui apusan tipis. Penambahan klorokuin pada parasit yang diradiasi memberikan respon menekan pertumbuhan parasit terjadi penurunan parasitemia pada semua pengenc...

Alat Pelindung Diri (APD) adalah kelengkapan yang digunakan untuk melindungi tubuh perawat saat b... more Alat Pelindung Diri (APD) adalah kelengkapan yang digunakan untuk melindungi tubuh perawat saat bekerja. Motivasi merupakan faktor pendorong bagi perawat untuk semangat bekerja. Penelitian bertujuan untuk mengetahui faktor-faktor yang mempengaruhi motivasi perawat dalam penggunaan Alat Pelindung Diri (APD) di Rumah Sakit Umum Daerah dr. Zainoel Abidin Banda Aceh. Jenis penelitian kuantitatif; survey analitik dengan desain cross sectional study. Populasi penelitian adalah 313 perawat, teknik pengambilan sampel menggunakan probability sampling, jumlah 73 perawat. Analisa data menggunakan uji Chi-Square. Hasil penelitian menunjukan ada hubungan faktor motivasi prestasi (p-value 0,026), tanggungjawab (p-value 0,008), pekerjaan (p-value 0,005), pengawasan (p-value 0,001), tempat kerja (p-value 0,000), keamanan kerja ( p-value 0,003), tidak ada hubungan motivasi gaji (p-valu 0,0613), kebijakan (p-value 0,0320), dengan penggunaan APD. Berdasarkan hasil penelitian diharapkan bagi perawat a...

Journal of environmental radioactivity, Jan 7, 2017

High expression of phospho histone γ-H2AX, a sensitive marker of double stranded DNA damage, is b... more High expression of phospho histone γ-H2AX, a sensitive marker of double stranded DNA damage, is believed to be an indication of defective DNA repair pathway or genomic instability that may cause mutations and ultimately cancer. DNA damage can be caused by ionizing radiation exposure. Beside in medical treatment/diagnosis or industry, ionizing radiation exposure can also be found in naturally in regions of high natural back ground radiation. In this study we collect the blood from 45 volunteers living in Mamuju, a region with highest natural radiation in Indonesia (dose of ∼7 mSv/year). Subjects were grouped as high natural background area (HNBA) (n = 37) and control area (n = 8). The expression γ-H2AX foci were evaluated by one of researcher fluorescence microscope examination. Our results show that the average foci numbers per cell were in the normal range. While not statistical different, the average of γ-H2AX foci in exposed area higher in the exposed compared to the control area...

Jurnal Sains dan Teknologi Nuklir Indonesia, 2018

EVALUASI HUBUNGAN DOSIS RADIASI TERHADAP KERUSAKAN DNA SEL LIMFOSIT DENGAN MENGGUNAKAN TES COME... more EVALUASI HUBUNGAN DOSIS RADIASI TERHADAP KERUSAKAN DNA SEL LIMFOSIT DENGAN MENGGUNAKAN TES COMET. Kerusakan asam nukleat (DNA) akibat radiasi atau faktor lain dapat mengarah ke efek yang lebih parah seperti sel kanker. Oleh karena itu diperlukan suatu metode deteksi untuk mengetahui besarnya kerusakan tersebut antara lain dengan metode uji komet yang sederhana. Tujuan penelitian ini adalah mengkaji efek radiasi pada sel limfosit akibat radiasi melalui teknik uji komet. Spesimen darah diiradiasi dosis gamma 2-8 Gy kemudian diisolasi limfositnya dengan histopaque. Isolat limfosit diproses uji komet pada slide dalam kondisi alkali dan hasil pencitraan dianalisis dengan software khusus. Hasil menunjukkan bahwa terdapat korelasi positif parameter komet dengan dosis radiasi, semakin besar dosis paparan semakin besar kerusakan DNA. Diantara parameter uji komet, tail DNA memberikan korelasi yang terbaik dengan dosis radiasi. Disimpulkan bahwa uji komet sangat baik digunakan untuk mengeta...

The two different biomarkers were used in this study, comets to see the effects of exposure and c... more The two different biomarkers were used in this study, comets to see the effects of exposure and chromosomal aberration to evaluate initial and residual lesions (not repaired or wrongly repaired) on lymphocytes of medical radiation workers. The purpose of this study is to provide preliminary data on the genotoxic implications resulting from chronic low-dose ionizing radiation exposure. A Primary DNA damage assessment was carried out using a comet alkaline test. The data obtained is then compared with the results of the chromosome aberration (CA) test. Genetic damage evaluation was carried out on 54 subjects (30 exposed and 24 controls). The results obtained in primary DNA damage, which was exposed indicating there was a significant increase in the comet length value of the p group <0.05,but the TM value was lower than in the control group although it was not significant. There was an increase in the frequency of CAs in workers compared to controls. The dicentric chromosome value o...

Gamma radiation can be used to attenuate the parasite. Weak parasite provides an opportunity for ... more Gamma radiation can be used to attenuate the parasite. Weak parasite provides an opportunity for the host to develop an adaptive immune response which is capable to reduce the risk of disease postinfection. The immune system against malaria parasites involves humoral and cellular components. Antibodies are one amongs the humoral components of immune system. The purpose of this study was to determine the effect of repeated immunization and challenge test on the antibody response and the growth of parasites in vivo. Conducted over 62 days period, this study used a randomized design with 60 Swiss Webster mices which were divided into three groups and. The radiation dose was set at 0 (positive control), 150, and 175 Gy. Immunization dose was set at 1 x 10 irradiated parasites per ml. Repeated immunization was performed sequentially at 1 and 2 weeks after the first immunization. Challenge test carried out at one month after the first immunization. Parasitic growth was observed every two ...

The aim of this study was to look at DNA damage studied in cancer patients during radiotherapy us... more The aim of this study was to look at DNA damage studied in cancer patients during radiotherapy using the comet test. Subjects were from patients diagnosed with different solid tumors who received radiotherapy and control groups adjusted for age, sex. Blood samples were collected from patients with several types of cancer after receiving cumulative radiation doses of 10, 12 and 14 Gy. The level of DNA damage was evaluated using the alkaline comet test on peripheral blood lymphocytes. Samples consisting of 3 ml of whole blood were collected by venipuncture. Isolation of lymphocytes was done using gradient centrifugation method. Evaluation on the capability of this compound in suppressing DNA damage was done by using alkaline Comet assay and The frequency of DNA cells was evaluated by calculating a total of 50 cells per slide. data analysis was done using CaspLab program. The comet test results showed that the average tail length, tail DNA, and tail moments of radiotherapy patients wer...

Atom Indonesia, 2019

The residents living in high background radiation area ha ve risk to be exposed by ionizing radia... more The residents living in high background radiation area ha ve risk to be exposed by ionizing radiation that also potential ly cause their DNA damage. The aim of this study was to determine the expression of γ - H2AX , 53BP1 foci and micronuclei in the resident s who live in high background radiation area of Salumati village , Mamuju, West Sulawesi , Indonesia. Twenty one blood samples which consist of 11 from the study area and 10 from control were assessed for their expression of γ - H2AX and 53BPI foci by using specific antibodies and observed under fluorescence microscope whereas m icronuclei was detected after being cultured and giemsa stain ed according to standard procedure s . Results showed that both γ-H2AX and 53BP1 foci from high background area was lower than that of control area (0.37± 0.24 vs 0.19± 0.11 (p=0.03) for γ-H2AX and 0.61± 0.30 vs 0.31± 0.12 for 53BP1 (p=0.01)). The m ean of m icronuclei frequency in exposed area was 0.02 (0,01-0,03) while in control area was 0...

Jurnal Bioteknologi & Biosains Indonesia (JBBI)

Analyses of DNA Damage in the Patient’s Lymphocyte Cells Post-Radiotherapy Radiotherapy given in ... more Analyses of DNA Damage in the Patient’s Lymphocyte Cells Post-Radiotherapy Radiotherapy given in high doses to kill cancer cells can also induce DNA damage in surrounding normal cells. The radiation dose is divided into smaller doses called fractionation to decrease the effect of radiation on normal tissue. For this reason, it is necessary to monitor the peripheral blood lymphocytes to evaluate the patient's DNA damage. The alkaline comet test is a simple and sensitive technique for detecting DNA instability. This study involved 11 patients who underwent radiotherapy up to 20 Gy, and 11 healthy subjects as controls. This study aims to see how much DNA damage is caused by a 20 Gy fractionated radiation dose in patients with various cancers. The results showed that the mean frequency of damaged cells in patients was 80.54 ± 12.52% with a mean comet tail length of 49.98 ± 12.93 µm. There was a significant difference in both the frequency of damaged cells and the mean value of the c...

Asian Pacific Journal of Cancer Prevention

Objective: The purpose of this study was to assess the immune response and malondialdehyde levels... more Objective: The purpose of this study was to assess the immune response and malondialdehyde levels in irradiated rats supplemented with Curcuma xanthorriza Roxb extract as a candidate for mitigating radiation exposure. Methods: Twenty-four male Wistar rats were grouped into eight treatment groups, then Curcuma xanthorrhiza Roxb extract was administered orally and irradiated at 6 Gy. Measurement of rats IL-6 and INF-γ was performed using a sandwich ELISA Kit, while the MDA concentration was quantified according to the method of Wills (1971). The statistical test is determined by one way ANOVA test. P-value <0.05 was considered statistically significant. Result: The concentration of IL-6 in all groups showed no statistically significant difference (P=0.18). There was an increase in the concentration of IL-6 in the group of rats irradiated with 6 Gy for 7 days and 14 days. Meanwhile, the INF-γ concentration also showed no significant results in all treatment groups (P=0.28). The average of MDA concentration showed a significant difference in the liver and spleen of irradiated rats at 6 Gy for 14 days compared to the control (0.044 nmol/mg vs 0.008 nmol/ mg, P=0.03 and 0.032 nmol/mg vs 0.014 nmol/mg, P=0.05, respectively). Conclusion: The administration of Curcuma xanthorriza Xorb extract was able to reduce MDA concentrations in the liver and spleen although not statistically significant. In addition, exposure to ionizing radiation at a dose of 6 Gy significantly increased lipid peroxidation in the liver and spleen by 5.5 times and 2.3 times, respectively.

Asian Pacific Journal of Cancer Prevention

Objective: The aim of this study was to assess the effect of radiation exposure, human 8-oxoguani... more Objective: The aim of this study was to assess the effect of radiation exposure, human 8-oxoguanine DNA N-glycosylase-1 (hOGG1) exon 7 genetic polymorphism and confounding factors on DNA damage response. Methods: Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and alkaline Comet assay method were applied to determine the hOGG1 genetic polymorphisms and DNA damage response. A total of 80 participants were enrolled in this study, consisting of 40 radiation-exposed workers as a case group and 40 non-radiation workers as a control group. Result: The genotypes frequencies for controls were Ser/Ser (35%), Ser/Cys (32.5%), and Cys/ Cys (32.5%), with frequencies of alleles being 326Ser (0.52) and 326Cys (0.48), whereas the genotypes frequencies for radiation-exposed workers (cases group) were Ser/Ser (17.5%), Ser/Cys (57.5%), and Cys/Cys (25%), with frequencies of alleles being 326Ser (0.46) and 326Cys (0.54). The results indicated that DNA damage response were not significantly higher in the exposed workers than in controls (22.55 ± 6.02 versus 21.72 ± 7.14; P=0.58). The time of exposure has a significantly negative correlation with comet tail length value among radiation workers. In addition, it was found that the DNA damage response was strongly associated with age and time of exposure with a decrease of 0.6 percent (P-value: 0.008) and 0.58 percent (P-value: 0.009), respectively. Whereas gender, smoking habit, and equivalent dose were not correlated with DNA damage. Conclusion: The single-nucleotide polymorphism of hOGG1 exon 7 (rs1052133) demonstrated no association with the extent of DNA damage in radiation-exposed workers.

Jurnal Forum Nuklir, 2014

EFEKTIFITAS KLOROKUIN TERHADAP PERTUMBUHAN Plasmodium falciparum RADlASI SECARA IN VITRO. Dalam u... more EFEKTIFITAS KLOROKUIN TERHADAP PERTUMBUHAN Plasmodium falciparum RADlASI SECARA IN VITRO. Dalam uji coba bahan vaksin malaria untuk mencegah relawan dari penyakit malaria maka digunakan chemoprophylaxis yang dikombinasikan dengan bahan vaksin yang akan diteliti. Klorokuin merupakan antimalaria yang umum digunakan sebagai chemoprophylaxis. Tujuan penelitian ini adalah untuk mengetahui efektivitas klorokuin terhadap pertumbuhan P. falciparum 3D7 radiasi. Pengujian Efektivitas klorokuin dilakukan dengan mengencerkan larutan stok 100 µg/ml sebesar 10-4, 10-5, 10-6, 10-7, 10-8 dan 10-9 . Masing-masing pengenceran dimasukkan ke dalam 40 µI suspensi sel darah merah terinfeksi parasit P. falciparum radiasi maupun yang tidak diradiasi dan diinkubasi pada 37°C. Jumlah parasit yang bertahan hidup dihitung setiap hari selama 7 hari melalui apusan tipis. Penambahan klorokuin pada parasit yang diradiasi memberikan respon menekan pertumbuhan parasit terjadi penurunan parasitemia pada semua pengenc...

Alat Pelindung Diri (APD) adalah kelengkapan yang digunakan untuk melindungi tubuh perawat saat b... more Alat Pelindung Diri (APD) adalah kelengkapan yang digunakan untuk melindungi tubuh perawat saat bekerja. Motivasi merupakan faktor pendorong bagi perawat untuk semangat bekerja. Penelitian bertujuan untuk mengetahui faktor-faktor yang mempengaruhi motivasi perawat dalam penggunaan Alat Pelindung Diri (APD) di Rumah Sakit Umum Daerah dr. Zainoel Abidin Banda Aceh. Jenis penelitian kuantitatif; survey analitik dengan desain cross sectional study. Populasi penelitian adalah 313 perawat, teknik pengambilan sampel menggunakan probability sampling, jumlah 73 perawat. Analisa data menggunakan uji Chi-Square. Hasil penelitian menunjukan ada hubungan faktor motivasi prestasi (p-value 0,026), tanggungjawab (p-value 0,008), pekerjaan (p-value 0,005), pengawasan (p-value 0,001), tempat kerja (p-value 0,000), keamanan kerja ( p-value 0,003), tidak ada hubungan motivasi gaji (p-valu 0,0613), kebijakan (p-value 0,0320), dengan penggunaan APD. Berdasarkan hasil penelitian diharapkan bagi perawat a...

Journal of environmental radioactivity, Jan 7, 2017

High expression of phospho histone γ-H2AX, a sensitive marker of double stranded DNA damage, is b... more High expression of phospho histone γ-H2AX, a sensitive marker of double stranded DNA damage, is believed to be an indication of defective DNA repair pathway or genomic instability that may cause mutations and ultimately cancer. DNA damage can be caused by ionizing radiation exposure. Beside in medical treatment/diagnosis or industry, ionizing radiation exposure can also be found in naturally in regions of high natural back ground radiation. In this study we collect the blood from 45 volunteers living in Mamuju, a region with highest natural radiation in Indonesia (dose of ∼7 mSv/year). Subjects were grouped as high natural background area (HNBA) (n = 37) and control area (n = 8). The expression γ-H2AX foci were evaluated by one of researcher fluorescence microscope examination. Our results show that the average foci numbers per cell were in the normal range. While not statistical different, the average of γ-H2AX foci in exposed area higher in the exposed compared to the control area...

Jurnal Sains dan Teknologi Nuklir Indonesia, 2018

EVALUASI HUBUNGAN DOSIS RADIASI TERHADAP KERUSAKAN DNA SEL LIMFOSIT DENGAN MENGGUNAKAN TES COME... more EVALUASI HUBUNGAN DOSIS RADIASI TERHADAP KERUSAKAN DNA SEL LIMFOSIT DENGAN MENGGUNAKAN TES COMET. Kerusakan asam nukleat (DNA) akibat radiasi atau faktor lain dapat mengarah ke efek yang lebih parah seperti sel kanker. Oleh karena itu diperlukan suatu metode deteksi untuk mengetahui besarnya kerusakan tersebut antara lain dengan metode uji komet yang sederhana. Tujuan penelitian ini adalah mengkaji efek radiasi pada sel limfosit akibat radiasi melalui teknik uji komet. Spesimen darah diiradiasi dosis gamma 2-8 Gy kemudian diisolasi limfositnya dengan histopaque. Isolat limfosit diproses uji komet pada slide dalam kondisi alkali dan hasil pencitraan dianalisis dengan software khusus. Hasil menunjukkan bahwa terdapat korelasi positif parameter komet dengan dosis radiasi, semakin besar dosis paparan semakin besar kerusakan DNA. Diantara parameter uji komet, tail DNA memberikan korelasi yang terbaik dengan dosis radiasi. Disimpulkan bahwa uji komet sangat baik digunakan untuk mengeta...

The two different biomarkers were used in this study, comets to see the effects of exposure and c... more The two different biomarkers were used in this study, comets to see the effects of exposure and chromosomal aberration to evaluate initial and residual lesions (not repaired or wrongly repaired) on lymphocytes of medical radiation workers. The purpose of this study is to provide preliminary data on the genotoxic implications resulting from chronic low-dose ionizing radiation exposure. A Primary DNA damage assessment was carried out using a comet alkaline test. The data obtained is then compared with the results of the chromosome aberration (CA) test. Genetic damage evaluation was carried out on 54 subjects (30 exposed and 24 controls). The results obtained in primary DNA damage, which was exposed indicating there was a significant increase in the comet length value of the p group <0.05,but the TM value was lower than in the control group although it was not significant. There was an increase in the frequency of CAs in workers compared to controls. The dicentric chromosome value o...

Gamma radiation can be used to attenuate the parasite. Weak parasite provides an opportunity for ... more Gamma radiation can be used to attenuate the parasite. Weak parasite provides an opportunity for the host to develop an adaptive immune response which is capable to reduce the risk of disease postinfection. The immune system against malaria parasites involves humoral and cellular components. Antibodies are one amongs the humoral components of immune system. The purpose of this study was to determine the effect of repeated immunization and challenge test on the antibody response and the growth of parasites in vivo. Conducted over 62 days period, this study used a randomized design with 60 Swiss Webster mices which were divided into three groups and. The radiation dose was set at 0 (positive control), 150, and 175 Gy. Immunization dose was set at 1 x 10 irradiated parasites per ml. Repeated immunization was performed sequentially at 1 and 2 weeks after the first immunization. Challenge test carried out at one month after the first immunization. Parasitic growth was observed every two ...

The aim of this study was to look at DNA damage studied in cancer patients during radiotherapy us... more The aim of this study was to look at DNA damage studied in cancer patients during radiotherapy using the comet test. Subjects were from patients diagnosed with different solid tumors who received radiotherapy and control groups adjusted for age, sex. Blood samples were collected from patients with several types of cancer after receiving cumulative radiation doses of 10, 12 and 14 Gy. The level of DNA damage was evaluated using the alkaline comet test on peripheral blood lymphocytes. Samples consisting of 3 ml of whole blood were collected by venipuncture. Isolation of lymphocytes was done using gradient centrifugation method. Evaluation on the capability of this compound in suppressing DNA damage was done by using alkaline Comet assay and The frequency of DNA cells was evaluated by calculating a total of 50 cells per slide. data analysis was done using CaspLab program. The comet test results showed that the average tail length, tail DNA, and tail moments of radiotherapy patients wer...

Atom Indonesia, 2019

The residents living in high background radiation area ha ve risk to be exposed by ionizing radia... more The residents living in high background radiation area ha ve risk to be exposed by ionizing radiation that also potential ly cause their DNA damage. The aim of this study was to determine the expression of γ - H2AX , 53BP1 foci and micronuclei in the resident s who live in high background radiation area of Salumati village , Mamuju, West Sulawesi , Indonesia. Twenty one blood samples which consist of 11 from the study area and 10 from control were assessed for their expression of γ - H2AX and 53BPI foci by using specific antibodies and observed under fluorescence microscope whereas m icronuclei was detected after being cultured and giemsa stain ed according to standard procedure s . Results showed that both γ-H2AX and 53BP1 foci from high background area was lower than that of control area (0.37± 0.24 vs 0.19± 0.11 (p=0.03) for γ-H2AX and 0.61± 0.30 vs 0.31± 0.12 for 53BP1 (p=0.01)). The m ean of m icronuclei frequency in exposed area was 0.02 (0,01-0,03) while in control area was 0...

Jurnal Bioteknologi & Biosains Indonesia (JBBI)

Analyses of DNA Damage in the Patient’s Lymphocyte Cells Post-Radiotherapy Radiotherapy given in ... more Analyses of DNA Damage in the Patient’s Lymphocyte Cells Post-Radiotherapy Radiotherapy given in high doses to kill cancer cells can also induce DNA damage in surrounding normal cells. The radiation dose is divided into smaller doses called fractionation to decrease the effect of radiation on normal tissue. For this reason, it is necessary to monitor the peripheral blood lymphocytes to evaluate the patient's DNA damage. The alkaline comet test is a simple and sensitive technique for detecting DNA instability. This study involved 11 patients who underwent radiotherapy up to 20 Gy, and 11 healthy subjects as controls. This study aims to see how much DNA damage is caused by a 20 Gy fractionated radiation dose in patients with various cancers. The results showed that the mean frequency of damaged cells in patients was 80.54 ± 12.52% with a mean comet tail length of 49.98 ± 12.93 µm. There was a significant difference in both the frequency of damaged cells and the mean value of the c...