Zainur Hakim - Academia.edu (original) (raw)

Papers by Zainur Hakim

Research paper thumbnail of Potential of The Tyrosinase Enzyme Inhibition by Standardized Ethanol Extract And Ethyl Acetate Fraction of Bengkoang Peel (Pachyrhizus erosus L.)

JURNAL ILMU KEFARMASIAN INDONESIA

This study examined the tyrosinase inhibitory potential of bengkoang peel standardised ethanolic ... more This study examined the tyrosinase inhibitory potential of bengkoang peel standardised ethanolic extract and ethyl acetate fractions. 450 g of bengkoang peel powder (Pachyrhizus erosus L.) was macerated with 96% ethanol for 2 x 24 h, filtered, and concentrated till viscous. Extract standardisation uses specific and non-specific parameters. Microplate colorimetric tyrosinase inhibition assays were performed. The result of plant determination identified the bengkoang peel as Pachyrhizus erosus (L.) Urb. The organoleptic test revealed a brownish-yellow viscous extract, fresh smell, and bitter taste. Ethanol-soluble extract was 47.60% and water-soluble 22.42%. Bengkoang peel ethanolic extract includes alkaloids, flavonoids, saponins, tannins, and steroids. The non-specific parameters showed loss of drying (9.71%), water content (7.28%), total ash (9.91%), acid-insoluble ash (3.68%), no residual solvent content, lead content (-0.2324±0.1729), cadmium content (-0.0841±0.3418), TPC of 1 x ...

Research paper thumbnail of Developing transdermal patch from Momordica charantia L. fruit using polyvinyl alcohol and ethyl cellulose potentially as anti-inflammatory dosage form

International Journal of Science and Technology Research Archive, Dec 30, 2022

Research paper thumbnail of CEK SIMILARITY "THE EFFECT OF ROSELLE ( Hibiscus Sabdariffa L.) CALYX ETHANOLIC EXTRACT ON GST ACTIVITY AND DECREASING OF ALT AND AST LEVEL ON 7,12 DIMETHYL BENZ(A) ANTRASEN (DMBA) INDUCED RATS

Background : Oxidants or free radicals caused all abnormalities in this human body and to make li... more Background : Oxidants or free radicals caused all abnormalities in this human body and to make liver cells damaged. Various natural products have been observing for drugs or compounds as antioxidants as Roselle ( Hibiscus sabdariffa L ). Objective: The aim of this study was to determine ethanolic extract of roselle protect Method: Roselle was extracted by maceration 60 %ethanol with a ratio of dry calyx and solvents are (1:3) stirer for 24 hours , then is filtered and concentrated by Rotary evaporator at 60°C . Maserate was transferred into a porcelain cup and evaporated on water bath at 60°C . Condensed extract is then stored in a sealed container and be identified .Forty-five Male Sprague Dawley rats age of 4 weeks divided into 9 groups: group I are baseline, group II and III was DMBA control group at day 7 and 35 (Single dose 75 mg/kgbw) . Group IV , V and VI are roselle extract treatment groups with variations each dose group of 10 mg / kg , 50 mg/kg and 100 mg/kgbw on day 7 ind...

Research paper thumbnail of Hasil Peer Review Teman Sejawat "THE EFFECT OF ROSELLE ( Hibiscus Sabdariffa L.) CALYX ETHANOLIC EXTRACT ON GST ACTIVITY AND DECREASING OF ALT AND AST LEVEL ON 7,12 DIMETHYL BENZ(A) ANTRASEN (DMBA) INDUCED RATS

Background : Oxidants or free radicals caused all abnormalities in this human body and to make li... more Background : Oxidants or free radicals caused all abnormalities in this human body and to make liver cells damaged. Various natural products have been observing for drugs or compounds as antioxidants as Roselle ( Hibiscus sabdariffa L ). Objective: The aim of this study was to determine ethanolic extract of roselle protect Method: Roselle was extracted by maceration 60 %ethanol with a ratio of dry calyx and solvents are (1:3) stirer for 24 hours , then is filtered and concentrated by Rotary evaporator at 60°C . Maserate was transferred into a porcelain cup and evaporated on water bath at 60°C . Condensed extract is then stored in a sealed container and be identified .Forty-five Male Sprague Dawley rats age of 4 weeks divided into 9 groups: group I are baseline, group II and III was DMBA control group at day 7 and 35 (Single dose 75 mg/kgbw) . Group IV , V and VI are roselle extract treatment groups with variations each dose group of 10 mg / kg , 50 mg/kg and 100 mg/kgbw on day 7 ind...

Research paper thumbnail of THE EFFECT OF ROSELLE ( Hibiscus Sabdariffa L.) CALYX ETHANOLIC EXTRACT ON GST ACTIVITY AND DECREASING OF ALT AND AST LEVEL ON 7,12 DIMETHYL BENZ(A) ANTRASEN (DMBA) INDUCED RATS

Background : Oxidants or free radicals caused all abnormalities in this human body and to make li... more Background : Oxidants or free radicals caused all abnormalities in this human body and to make liver cells damaged. Various natural products have been observing for drugs or compounds as antioxidants as Roselle ( Hibiscus sabdariffa L ). Objective: The aim of this study was to determine ethanolic extract of roselle protect Method: Roselle was extracted by maceration 60 %ethanol with a ratio of dry calyx and solvents are (1:3) stirer for 24 hours , then is filtered and concentrated by Rotary evaporator at 60°C . Maserate was transferred into a porcelain cup and evaporated on water bath at 60°C . Condensed extract is then stored in a sealed container and be identified .Forty-five Male Sprague Dawley rats age of 4 weeks divided into 9 groups: group I are baseline, group II and III was DMBA control group at day 7 and 35 (Single dose 75 mg/kgbw) . Group IV , V and VI are roselle extract treatment groups with variations each dose group of 10 mg / kg , 50 mg/kg and 100 mg/kgbw on day 7 ind...

Research paper thumbnail of UJI INTERAKSI METFORMIN DAN GLIBENKLAMID DENGAN JAMU YANG MENGANDUNG Smallanthus sonchifolius DAN KOMBINASI Andrographis paniculata (Burm. F), Boesenbergia pandurata Roxb, Phyllanthus niruri L, Syzigii polyanthi Wight PADA PENURUNAN KADAR GLUKOSA DARAH TI

JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X

Penggunaan jamu sebagai Complementary and Alternative Medicine untuk mengatasi Diabetes Mellitus ... more Penggunaan jamu sebagai Complementary and Alternative Medicine untuk mengatasi Diabetes Mellitus banyak digunakan di Indonesia. Potensi munculnya interaksi obat-jamu dengan metformin dan glibenklamid dapat mempengaruhi efektivitas menurunkan kadar glukosa darah. Penelitian ini bertujuan untuk mengkaji interaksi farmakodinamika (aktivitas farmakologi) dari metformin dan glibenklamid yang dikombinasikan dengan jamu A (Smallanthus sonchifolius) dan jamu B (Andrographis paniculata (Burm. F), Boesenbergia pandurata Roxb, Phyllanthus niruri L, Syzigii polyanthi) dalam menurunkan kadar glukosa darah tikus yang diinduksi aloksan. Metode KLT-Densitometer digunakan untuk skrining fitokimia dengan membandingkan profil kromatogram (nilai Rf) dari jamu dan ekstrak etanol tanaman. Sebanyak 30 ekor tikus wistar jantan dibagi menjadi 10 kelompok perlakuan dengan aloksan 175 mg/Kg BB. Pengamatan kadar glukosa darah diukur pada hari ke-0,-7,-14, dan -21 setelah tikus dipuasakan 12 jam. Hasil profil...

Research paper thumbnail of Anti-Inflammatory and Analgesic Activity of Musa balbisiana Peels In Vivo

Borneo Journal of Pharmacy

Musa balbisiana Peels (MBP) contains high levels of flavonoids, alkaloids, tannins, saponins, and... more Musa balbisiana Peels (MBP) contains high levels of flavonoids, alkaloids, tannins, saponins, and triterpenoids. Flavonoids function to slow down the inflammatory process by inhibiting the arachidonic acid, forming prostaglandins, and releasing histamine. This study aimed to examine the anti-inflammatory and analgesic effects of MBP decoction. This study used the Winter method for anti-inflammatory assay by induction of carrageenan on the soles of rat's feet and Sigmund's method for analgesic assay with intraperitoneal induction of acetic acid in mice. Group I as a negative control, group II as a positive control with diclofenac sodium, group III as a low dose (200 mg/kg BW of MBP), group IV as a medium dose (400 mg/kg BW of MBP), and group V as a high dose (800 mg/kg BW of MBP decoction). The percentage of inhibition in the anti-inflammatory test in rats for groups II, III, IV, and V was 34.43%, 17.68%, 25.53%, and 25.4%, and the percentage of effectiveness for the anti-inf...

Research paper thumbnail of Efektivitas Intervensi Murotal dan Musik Instrumental pada Pasien Hipertensi

Prosiding Artikel Seminar Nasional Farmasi, 2019

Research paper thumbnail of Pelatihan Pencegahan Dan Pengobatan Penyakit Degeneratif Dengan Jamu Saintifik Pada Kader Aisyiah Desa Pamijen

PROSIDING SEMINAR NASIONAL LPPM UMP, Feb 10, 2021

Research paper thumbnail of Effect of rosella (Hibiscus sabdariffa L) extract on glutathione-S-transferase activity in rats

Tropical Journal of Pharmaceutical Research, 2017

Purpose: To determine the effect of rosella (Hibiscus sabdariffa L) extract on glutathione-S-tra... more Purpose: To determine the effect of rosella (Hibiscus sabdariffa L) extract on glutathione-S-trasferase (GST) activity and its hepatoprotective effect.Methods: A total of 25 rats were divided randomly into 5 groups (5 rats per group). Group I served as the baseline, group II was the negative control group, while groups III, IV and V were treated with rosella extract at doses of 10, 50 and 100 mg /kg /day, respectively for 35 days. On day 36, the animals were given a single dose of dimethyl benz(a)anthracene (DMBA) orally. After one week, blood was taken from the sinus orbitalis for measurement of serum glutamate pyruvate transaminase (SGPT) and serum glutamate oxaloacetate transaminase (SGOT) activities by a kinetic method with DIasys® kit. The activity of GST was measured in liver homogenate using 1-choloro-2,4-dinitrobenzene (CDNB) method, while the expression of GST gene was determined by reverse transcriptase polymerase chain reaction (RT-PCR).Results: Treatment with rosella ...

Research paper thumbnail of Penyuluhan Dan Pembuatan Jamu Saintifik Sebagai Salah Satu Alternatif Pengobatan Penyakit Asam Urat Warga Muhammadiyah Desa Pliken

Pengetahuan masyarakat akan penggunaan jamu sebagai alternatif dan pendamping pengobatan sangat r... more Pengetahuan masyarakat akan penggunaan jamu sebagai alternatif dan pendamping pengobatan sangat rendah dan belum terlalu populer di masyarakat. Kementerian Kesehatan melalui Badan Penelitian dan Pengembangan Kesehatan menjalankan program saintifikasi jamu (SJ) berdasarkan Peraturan Kementerian Kesehatan RI No.003/PerMenKes/I/2010 telah membuktikan khasiat jamu dengan metode penelitian berbasis pelayanan. Sehingga potensi pengobatan dengan jamu ini bisa disosialisasikan kepada masyarakat untuk menekan potensi peningkatan penderita penyakit asam urat pada warga muhammadiyah dan sekitar wilayah desa Pliken, Kembaran, Banyumas. Metode yang digunakan yaitu penyuluhan terkait dengan potensi penyakit, patofisiologi penyakit, pengobatan konvnsional terkini, deteksi dini, dan cara pencegahannya baik dengan perbaikan pola makan dan hidup sehat serta mengkonsumsi jamu sebagai alternatif pengobatan asam urat. Praktek pembuatan jamu saintifik dengan memperkenalkan bahan baku herbal dan cara peng...

Research paper thumbnail of Annona muricata L . ) leaves and determination of its antioxidant activity )

The ethanolic extract of soursop leaves (Annona muricata L.) is reported as an antioxidant agent ... more The ethanolic extract of soursop leaves (Annona muricata L.) is reported as an antioxidant agent with an IC50 value of 14.48 μg/mL. This research aimed to examine physical characteristics and investigate the antioxidant activity of the ethanolic extract of soursop leaves in a scrub cream. Soursop leaves powder was extracted with 70% ethanol and formulated as scrub cream at the concentration of 1.4%, 2.8%, and 4.2%, then evaluated for physical characteristics. The antioxidant activity was evaluated using DPPH (1.1-diphenyl-2 picrylhydrazyl) method. The result showed that the scrub cream at all concentrations tested fulfilled all physical characteristics required for good topical preparations. These included organoleptic, homogeneity, viscosity, pH, spreadability, and adhesion. The IC50 value of the scrub cream at the concentration of 1.4%, 2.8 %, and 4.2% were 30.72, 26.82, and 24.03 μg/mL, respectively. The IC50 values indicated that the scrub creams exhibit radical scavenging activ...

Research paper thumbnail of Formulasi, Evaluasi Sifat Fisik, dan Uji Efektivitas Tabir Surya Losion Ekstrak Buah Jamblang (Syzygium cumini (L.) Skeels)

PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia), 2020

Angka kejadian kanker kulit yang dijumpai di Indonesia cukup tinggi sekitar 5,9-7,8%. Salah satu ... more Angka kejadian kanker kulit yang dijumpai di Indonesia cukup tinggi sekitar 5,9-7,8%. Salah satu penyebabnya adalah paparan sinar UV berlebihan pada kulit. Dengan demikian diperlukan adanya suatu produk yang dapat melindungi kulit dari bahaya sinar UV. Pada penelitian terdahulu buah jamblang dilaporkan memiliki nilai faktor perlindungan terhadap matahari (sun protection factor/SPF) yaitu 2,278±0,25% yang berpotensi untuk dibuat sediaan tabir surya. Penelitian ini bertujuan untuk membuat formula losion ekstrak buah jamblang (Syzygium cumini (L.) Skeels) yang kemudian diuji karakteristik fisik dan efektivitas tabir surya dari produk tersebut. Buah jamblang kering dihaluskan dan diayak pada 40/60 mesh sehingga didapatkan serbuk simplisia dan dihitung rendemennya. Sebanyak 100 g serbuk diekstraksi dengan metode remaserasi dalam pelarut etanol 70% selama 3x24 jam dan ditambahkan secukupnya HCl 37% hingga pH 1. Maserat kemudian disaring dan dipekatkan dengan rotary evaporator sampai diper...

Research paper thumbnail of Formulasi dan Uji Sifat Fisik Sediaan Lulur Krim dari Ekstrak Etanol Daun Sirsak (Annona muricata L.) serta Penentuan Aktivitas Antioksidannya

Jurnal Sains Farmasi & Klinis, 2020

Ekstrak etanol daun sirsak (Annona muricata L.) dilaporkan memiliki aktivitas antioksidan dengan ... more Ekstrak etanol daun sirsak (Annona muricata L.) dilaporkan memiliki aktivitas antioksidan dengan nilai konsentrasi hambat 50 (IC50) sebesar 14,48 µg/mL. Penelitian ini bertujuan untuk mengetahui sifat fisik dan aktivitas antioksidan dari lulur krim ekstrak etanol daun sirsak. Serbuk kering daun sirsak diekstraksi dengan etanol 70% kemudian dibuat formula lulur krim dengan konsentrasi 1,4%, 2,8%, dan 4,2% lalu dilakukan uji sifat fisiknya. Aktivitas antioksidan ditentukan dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). Hasil penelitian menunjukkan bahwa lulur krim ekstrak daun sirsak pada semua konsentrasi memenuhi semua persyaratan uji sifat fisik sediaan topikal yang baik. Hal ini meliputi organoleptis, homogenitas, viskositas, pH, daya sebar dan daya lekat. Nilai IC50 lulur krim ekstrak etanol daun sirsak dengan konsentrasi 1,4%; 2,8%; dan 4,2% adalah 30,72; 26,82; dan 24,03 µg/mL. Hal ini menunjukkan bahwa sediaan lulur krim ekstrak daun sirsak memiliki aktivitas antioksidan ...

Research paper thumbnail of Phytochemical analysis, FTIR fingerprint and bioactivity of crude extract and fractions of Mesua ferrea L

Research Journal of Life Science, 2019

Mesua ferrea L. (Ceylon ironwood, Calophyllaceae) is a popular medicinal plant with a long histor... more Mesua ferrea L. (Ceylon ironwood, Calophyllaceae) is a popular medicinal plant with a long history of use in South-and Southeast Asian folk medicines. In this study, the qualitative phytochemical analysis, FTIR fingerprint, as well as antimicrobial and antioxidant activities of the crude extract of M. ferrea leaves and its fractions were reported. The dried leaves of M. ferrea were extracted with ethanol by the re-maceration method. The crude extract was further partitioned in ethanol-water and ethyl acetate to obtain ethanol and ethyl acetate fractions, respectively. The identification of compounds in the extract and fractions was conducted according to the standard phytochemical screening method. Fourier-transform infrared (FTIR) spectroscopy was utilized to record the metabolites fingerprint of the extract and its fractions. The antimicrobial activity was evaluated with the disk diffusion method against Escherichia coli, Bacillus subtilis, Candida albicans, and Saccharomyces cerevisiae. The antioxidant activity assay was determined with 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. The crude extract of M. ferrea leaves and its fractions contained saponins, phenolic compounds, flavonoids, and terpenoids. The FTIR data supported the presence of terpenoids and glycosides in those samples. The extract and fractions demonstrated considerable antimicrobial activity against all tested bacteria and fungi, with MIC values were 3.9-31.3 μg/ml. The ethyl acetate fraction of M. ferrea leaves showed the best antioxidant activity with an IC50 value of 49.19 μg/ml. M. ferrea is used as deodorant, diaphoretic and stimulant, as well as a brain tonic, appetizer, antiemetic, anthelminthic, aphrodisiac, diuretic and antidote (Suresh, Chandra, Meeta, & Deep, 2014). It is the identity flora of Banyumas, and traditionally is used for the treatment of leukemia, mental illness, liver disorder, dengue fever, and heart disease by people in Baturraden, Banyumas (Suparman, Diniatik, Kusumaningrum, & Yulianto, 2012). In Pidie, Nanggroe Aceh Darussalam, M. ferrea is used as one of the ingredients of post-natal concoction for the mothers (Viena, Yunita, Irhamni, Saudah, & Ernilasari, 2018). The extracts, fractions, and isolates of M. ferrea have been evaluated for various KEYWORDS Bioactivities Extract Fractions Mesua ferrea L. FTIR fingerprint

Research paper thumbnail of Aktivitas Immunodulator Ekstrak Etanol Umbi Bawang Merah (Allium cepa L.) terhadap Respon Imun Non Spesifik pada Mencit Jantan Galur BALB/C dengan Metode Carbon Clearance

Biosfera, 2017

The content of flavonoids on onion useful to maintain endurance. This research aims to know the a... more The content of flavonoids on onion useful to maintain endurance. This research aims to know the activity of exstract immunodulator of onion bulb ethanol (Allium cepa L) on phagocytic activity in male mice balb/c with varian dose and campored immunodulator activity with positive control. This study is an experimental reseach with Completely Randomized Design (CRD). Preparation of extract ethanol onion roots done by maceration using 96% ethanol for 9 hours. The test performed on samples which consist of 25 mice with carbon clearance method, devided into 5 groups, which are : K – (without treatment), k+ (with imboos 13,3 ml/Kg Bw), P1 (dose 12%), P2 (dose 24%), and P3 (dose 48%). The test performed for 5 days, and on the 7th day injected carbon (pelicans) as much 0,1 ml/ 10 gr Weight intravenousty, their blood taken before carbon injection (minute 0) and minute 4, 8, 12, and 16. After their transmittance measure using spectrophotometer at 600,5 nm wavelength. This research shows that e...

Research paper thumbnail of Potential of The Tyrosinase Enzyme Inhibition by Standardized Ethanol Extract And Ethyl Acetate Fraction of Bengkoang Peel (Pachyrhizus erosus L.)

JURNAL ILMU KEFARMASIAN INDONESIA

This study examined the tyrosinase inhibitory potential of bengkoang peel standardised ethanolic ... more This study examined the tyrosinase inhibitory potential of bengkoang peel standardised ethanolic extract and ethyl acetate fractions. 450 g of bengkoang peel powder (Pachyrhizus erosus L.) was macerated with 96% ethanol for 2 x 24 h, filtered, and concentrated till viscous. Extract standardisation uses specific and non-specific parameters. Microplate colorimetric tyrosinase inhibition assays were performed. The result of plant determination identified the bengkoang peel as Pachyrhizus erosus (L.) Urb. The organoleptic test revealed a brownish-yellow viscous extract, fresh smell, and bitter taste. Ethanol-soluble extract was 47.60% and water-soluble 22.42%. Bengkoang peel ethanolic extract includes alkaloids, flavonoids, saponins, tannins, and steroids. The non-specific parameters showed loss of drying (9.71%), water content (7.28%), total ash (9.91%), acid-insoluble ash (3.68%), no residual solvent content, lead content (-0.2324±0.1729), cadmium content (-0.0841±0.3418), TPC of 1 x ...

Research paper thumbnail of Developing transdermal patch from Momordica charantia L. fruit using polyvinyl alcohol and ethyl cellulose potentially as anti-inflammatory dosage form

International Journal of Science and Technology Research Archive, Dec 30, 2022

Research paper thumbnail of CEK SIMILARITY "THE EFFECT OF ROSELLE ( Hibiscus Sabdariffa L.) CALYX ETHANOLIC EXTRACT ON GST ACTIVITY AND DECREASING OF ALT AND AST LEVEL ON 7,12 DIMETHYL BENZ(A) ANTRASEN (DMBA) INDUCED RATS

Background : Oxidants or free radicals caused all abnormalities in this human body and to make li... more Background : Oxidants or free radicals caused all abnormalities in this human body and to make liver cells damaged. Various natural products have been observing for drugs or compounds as antioxidants as Roselle ( Hibiscus sabdariffa L ). Objective: The aim of this study was to determine ethanolic extract of roselle protect Method: Roselle was extracted by maceration 60 %ethanol with a ratio of dry calyx and solvents are (1:3) stirer for 24 hours , then is filtered and concentrated by Rotary evaporator at 60°C . Maserate was transferred into a porcelain cup and evaporated on water bath at 60°C . Condensed extract is then stored in a sealed container and be identified .Forty-five Male Sprague Dawley rats age of 4 weeks divided into 9 groups: group I are baseline, group II and III was DMBA control group at day 7 and 35 (Single dose 75 mg/kgbw) . Group IV , V and VI are roselle extract treatment groups with variations each dose group of 10 mg / kg , 50 mg/kg and 100 mg/kgbw on day 7 ind...

Research paper thumbnail of Hasil Peer Review Teman Sejawat "THE EFFECT OF ROSELLE ( Hibiscus Sabdariffa L.) CALYX ETHANOLIC EXTRACT ON GST ACTIVITY AND DECREASING OF ALT AND AST LEVEL ON 7,12 DIMETHYL BENZ(A) ANTRASEN (DMBA) INDUCED RATS

Background : Oxidants or free radicals caused all abnormalities in this human body and to make li... more Background : Oxidants or free radicals caused all abnormalities in this human body and to make liver cells damaged. Various natural products have been observing for drugs or compounds as antioxidants as Roselle ( Hibiscus sabdariffa L ). Objective: The aim of this study was to determine ethanolic extract of roselle protect Method: Roselle was extracted by maceration 60 %ethanol with a ratio of dry calyx and solvents are (1:3) stirer for 24 hours , then is filtered and concentrated by Rotary evaporator at 60°C . Maserate was transferred into a porcelain cup and evaporated on water bath at 60°C . Condensed extract is then stored in a sealed container and be identified .Forty-five Male Sprague Dawley rats age of 4 weeks divided into 9 groups: group I are baseline, group II and III was DMBA control group at day 7 and 35 (Single dose 75 mg/kgbw) . Group IV , V and VI are roselle extract treatment groups with variations each dose group of 10 mg / kg , 50 mg/kg and 100 mg/kgbw on day 7 ind...

Research paper thumbnail of THE EFFECT OF ROSELLE ( Hibiscus Sabdariffa L.) CALYX ETHANOLIC EXTRACT ON GST ACTIVITY AND DECREASING OF ALT AND AST LEVEL ON 7,12 DIMETHYL BENZ(A) ANTRASEN (DMBA) INDUCED RATS

Background : Oxidants or free radicals caused all abnormalities in this human body and to make li... more Background : Oxidants or free radicals caused all abnormalities in this human body and to make liver cells damaged. Various natural products have been observing for drugs or compounds as antioxidants as Roselle ( Hibiscus sabdariffa L ). Objective: The aim of this study was to determine ethanolic extract of roselle protect Method: Roselle was extracted by maceration 60 %ethanol with a ratio of dry calyx and solvents are (1:3) stirer for 24 hours , then is filtered and concentrated by Rotary evaporator at 60°C . Maserate was transferred into a porcelain cup and evaporated on water bath at 60°C . Condensed extract is then stored in a sealed container and be identified .Forty-five Male Sprague Dawley rats age of 4 weeks divided into 9 groups: group I are baseline, group II and III was DMBA control group at day 7 and 35 (Single dose 75 mg/kgbw) . Group IV , V and VI are roselle extract treatment groups with variations each dose group of 10 mg / kg , 50 mg/kg and 100 mg/kgbw on day 7 ind...

Research paper thumbnail of UJI INTERAKSI METFORMIN DAN GLIBENKLAMID DENGAN JAMU YANG MENGANDUNG Smallanthus sonchifolius DAN KOMBINASI Andrographis paniculata (Burm. F), Boesenbergia pandurata Roxb, Phyllanthus niruri L, Syzigii polyanthi Wight PADA PENURUNAN KADAR GLUKOSA DARAH TI

JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X

Penggunaan jamu sebagai Complementary and Alternative Medicine untuk mengatasi Diabetes Mellitus ... more Penggunaan jamu sebagai Complementary and Alternative Medicine untuk mengatasi Diabetes Mellitus banyak digunakan di Indonesia. Potensi munculnya interaksi obat-jamu dengan metformin dan glibenklamid dapat mempengaruhi efektivitas menurunkan kadar glukosa darah. Penelitian ini bertujuan untuk mengkaji interaksi farmakodinamika (aktivitas farmakologi) dari metformin dan glibenklamid yang dikombinasikan dengan jamu A (Smallanthus sonchifolius) dan jamu B (Andrographis paniculata (Burm. F), Boesenbergia pandurata Roxb, Phyllanthus niruri L, Syzigii polyanthi) dalam menurunkan kadar glukosa darah tikus yang diinduksi aloksan. Metode KLT-Densitometer digunakan untuk skrining fitokimia dengan membandingkan profil kromatogram (nilai Rf) dari jamu dan ekstrak etanol tanaman. Sebanyak 30 ekor tikus wistar jantan dibagi menjadi 10 kelompok perlakuan dengan aloksan 175 mg/Kg BB. Pengamatan kadar glukosa darah diukur pada hari ke-0,-7,-14, dan -21 setelah tikus dipuasakan 12 jam. Hasil profil...

Research paper thumbnail of Anti-Inflammatory and Analgesic Activity of Musa balbisiana Peels In Vivo

Borneo Journal of Pharmacy

Musa balbisiana Peels (MBP) contains high levels of flavonoids, alkaloids, tannins, saponins, and... more Musa balbisiana Peels (MBP) contains high levels of flavonoids, alkaloids, tannins, saponins, and triterpenoids. Flavonoids function to slow down the inflammatory process by inhibiting the arachidonic acid, forming prostaglandins, and releasing histamine. This study aimed to examine the anti-inflammatory and analgesic effects of MBP decoction. This study used the Winter method for anti-inflammatory assay by induction of carrageenan on the soles of rat's feet and Sigmund's method for analgesic assay with intraperitoneal induction of acetic acid in mice. Group I as a negative control, group II as a positive control with diclofenac sodium, group III as a low dose (200 mg/kg BW of MBP), group IV as a medium dose (400 mg/kg BW of MBP), and group V as a high dose (800 mg/kg BW of MBP decoction). The percentage of inhibition in the anti-inflammatory test in rats for groups II, III, IV, and V was 34.43%, 17.68%, 25.53%, and 25.4%, and the percentage of effectiveness for the anti-inf...

Research paper thumbnail of Efektivitas Intervensi Murotal dan Musik Instrumental pada Pasien Hipertensi

Prosiding Artikel Seminar Nasional Farmasi, 2019

Research paper thumbnail of Pelatihan Pencegahan Dan Pengobatan Penyakit Degeneratif Dengan Jamu Saintifik Pada Kader Aisyiah Desa Pamijen

PROSIDING SEMINAR NASIONAL LPPM UMP, Feb 10, 2021

Research paper thumbnail of Effect of rosella (Hibiscus sabdariffa L) extract on glutathione-S-transferase activity in rats

Tropical Journal of Pharmaceutical Research, 2017

Purpose: To determine the effect of rosella (Hibiscus sabdariffa L) extract on glutathione-S-tra... more Purpose: To determine the effect of rosella (Hibiscus sabdariffa L) extract on glutathione-S-trasferase (GST) activity and its hepatoprotective effect.Methods: A total of 25 rats were divided randomly into 5 groups (5 rats per group). Group I served as the baseline, group II was the negative control group, while groups III, IV and V were treated with rosella extract at doses of 10, 50 and 100 mg /kg /day, respectively for 35 days. On day 36, the animals were given a single dose of dimethyl benz(a)anthracene (DMBA) orally. After one week, blood was taken from the sinus orbitalis for measurement of serum glutamate pyruvate transaminase (SGPT) and serum glutamate oxaloacetate transaminase (SGOT) activities by a kinetic method with DIasys® kit. The activity of GST was measured in liver homogenate using 1-choloro-2,4-dinitrobenzene (CDNB) method, while the expression of GST gene was determined by reverse transcriptase polymerase chain reaction (RT-PCR).Results: Treatment with rosella ...

Research paper thumbnail of Penyuluhan Dan Pembuatan Jamu Saintifik Sebagai Salah Satu Alternatif Pengobatan Penyakit Asam Urat Warga Muhammadiyah Desa Pliken

Pengetahuan masyarakat akan penggunaan jamu sebagai alternatif dan pendamping pengobatan sangat r... more Pengetahuan masyarakat akan penggunaan jamu sebagai alternatif dan pendamping pengobatan sangat rendah dan belum terlalu populer di masyarakat. Kementerian Kesehatan melalui Badan Penelitian dan Pengembangan Kesehatan menjalankan program saintifikasi jamu (SJ) berdasarkan Peraturan Kementerian Kesehatan RI No.003/PerMenKes/I/2010 telah membuktikan khasiat jamu dengan metode penelitian berbasis pelayanan. Sehingga potensi pengobatan dengan jamu ini bisa disosialisasikan kepada masyarakat untuk menekan potensi peningkatan penderita penyakit asam urat pada warga muhammadiyah dan sekitar wilayah desa Pliken, Kembaran, Banyumas. Metode yang digunakan yaitu penyuluhan terkait dengan potensi penyakit, patofisiologi penyakit, pengobatan konvnsional terkini, deteksi dini, dan cara pencegahannya baik dengan perbaikan pola makan dan hidup sehat serta mengkonsumsi jamu sebagai alternatif pengobatan asam urat. Praktek pembuatan jamu saintifik dengan memperkenalkan bahan baku herbal dan cara peng...

Research paper thumbnail of Annona muricata L . ) leaves and determination of its antioxidant activity )

The ethanolic extract of soursop leaves (Annona muricata L.) is reported as an antioxidant agent ... more The ethanolic extract of soursop leaves (Annona muricata L.) is reported as an antioxidant agent with an IC50 value of 14.48 μg/mL. This research aimed to examine physical characteristics and investigate the antioxidant activity of the ethanolic extract of soursop leaves in a scrub cream. Soursop leaves powder was extracted with 70% ethanol and formulated as scrub cream at the concentration of 1.4%, 2.8%, and 4.2%, then evaluated for physical characteristics. The antioxidant activity was evaluated using DPPH (1.1-diphenyl-2 picrylhydrazyl) method. The result showed that the scrub cream at all concentrations tested fulfilled all physical characteristics required for good topical preparations. These included organoleptic, homogeneity, viscosity, pH, spreadability, and adhesion. The IC50 value of the scrub cream at the concentration of 1.4%, 2.8 %, and 4.2% were 30.72, 26.82, and 24.03 μg/mL, respectively. The IC50 values indicated that the scrub creams exhibit radical scavenging activ...

Research paper thumbnail of Formulasi, Evaluasi Sifat Fisik, dan Uji Efektivitas Tabir Surya Losion Ekstrak Buah Jamblang (Syzygium cumini (L.) Skeels)

PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia), 2020

Angka kejadian kanker kulit yang dijumpai di Indonesia cukup tinggi sekitar 5,9-7,8%. Salah satu ... more Angka kejadian kanker kulit yang dijumpai di Indonesia cukup tinggi sekitar 5,9-7,8%. Salah satu penyebabnya adalah paparan sinar UV berlebihan pada kulit. Dengan demikian diperlukan adanya suatu produk yang dapat melindungi kulit dari bahaya sinar UV. Pada penelitian terdahulu buah jamblang dilaporkan memiliki nilai faktor perlindungan terhadap matahari (sun protection factor/SPF) yaitu 2,278±0,25% yang berpotensi untuk dibuat sediaan tabir surya. Penelitian ini bertujuan untuk membuat formula losion ekstrak buah jamblang (Syzygium cumini (L.) Skeels) yang kemudian diuji karakteristik fisik dan efektivitas tabir surya dari produk tersebut. Buah jamblang kering dihaluskan dan diayak pada 40/60 mesh sehingga didapatkan serbuk simplisia dan dihitung rendemennya. Sebanyak 100 g serbuk diekstraksi dengan metode remaserasi dalam pelarut etanol 70% selama 3x24 jam dan ditambahkan secukupnya HCl 37% hingga pH 1. Maserat kemudian disaring dan dipekatkan dengan rotary evaporator sampai diper...

Research paper thumbnail of Formulasi dan Uji Sifat Fisik Sediaan Lulur Krim dari Ekstrak Etanol Daun Sirsak (Annona muricata L.) serta Penentuan Aktivitas Antioksidannya

Jurnal Sains Farmasi & Klinis, 2020

Ekstrak etanol daun sirsak (Annona muricata L.) dilaporkan memiliki aktivitas antioksidan dengan ... more Ekstrak etanol daun sirsak (Annona muricata L.) dilaporkan memiliki aktivitas antioksidan dengan nilai konsentrasi hambat 50 (IC50) sebesar 14,48 µg/mL. Penelitian ini bertujuan untuk mengetahui sifat fisik dan aktivitas antioksidan dari lulur krim ekstrak etanol daun sirsak. Serbuk kering daun sirsak diekstraksi dengan etanol 70% kemudian dibuat formula lulur krim dengan konsentrasi 1,4%, 2,8%, dan 4,2% lalu dilakukan uji sifat fisiknya. Aktivitas antioksidan ditentukan dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). Hasil penelitian menunjukkan bahwa lulur krim ekstrak daun sirsak pada semua konsentrasi memenuhi semua persyaratan uji sifat fisik sediaan topikal yang baik. Hal ini meliputi organoleptis, homogenitas, viskositas, pH, daya sebar dan daya lekat. Nilai IC50 lulur krim ekstrak etanol daun sirsak dengan konsentrasi 1,4%; 2,8%; dan 4,2% adalah 30,72; 26,82; dan 24,03 µg/mL. Hal ini menunjukkan bahwa sediaan lulur krim ekstrak daun sirsak memiliki aktivitas antioksidan ...

Research paper thumbnail of Phytochemical analysis, FTIR fingerprint and bioactivity of crude extract and fractions of Mesua ferrea L

Research Journal of Life Science, 2019

Mesua ferrea L. (Ceylon ironwood, Calophyllaceae) is a popular medicinal plant with a long histor... more Mesua ferrea L. (Ceylon ironwood, Calophyllaceae) is a popular medicinal plant with a long history of use in South-and Southeast Asian folk medicines. In this study, the qualitative phytochemical analysis, FTIR fingerprint, as well as antimicrobial and antioxidant activities of the crude extract of M. ferrea leaves and its fractions were reported. The dried leaves of M. ferrea were extracted with ethanol by the re-maceration method. The crude extract was further partitioned in ethanol-water and ethyl acetate to obtain ethanol and ethyl acetate fractions, respectively. The identification of compounds in the extract and fractions was conducted according to the standard phytochemical screening method. Fourier-transform infrared (FTIR) spectroscopy was utilized to record the metabolites fingerprint of the extract and its fractions. The antimicrobial activity was evaluated with the disk diffusion method against Escherichia coli, Bacillus subtilis, Candida albicans, and Saccharomyces cerevisiae. The antioxidant activity assay was determined with 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. The crude extract of M. ferrea leaves and its fractions contained saponins, phenolic compounds, flavonoids, and terpenoids. The FTIR data supported the presence of terpenoids and glycosides in those samples. The extract and fractions demonstrated considerable antimicrobial activity against all tested bacteria and fungi, with MIC values were 3.9-31.3 μg/ml. The ethyl acetate fraction of M. ferrea leaves showed the best antioxidant activity with an IC50 value of 49.19 μg/ml. M. ferrea is used as deodorant, diaphoretic and stimulant, as well as a brain tonic, appetizer, antiemetic, anthelminthic, aphrodisiac, diuretic and antidote (Suresh, Chandra, Meeta, & Deep, 2014). It is the identity flora of Banyumas, and traditionally is used for the treatment of leukemia, mental illness, liver disorder, dengue fever, and heart disease by people in Baturraden, Banyumas (Suparman, Diniatik, Kusumaningrum, & Yulianto, 2012). In Pidie, Nanggroe Aceh Darussalam, M. ferrea is used as one of the ingredients of post-natal concoction for the mothers (Viena, Yunita, Irhamni, Saudah, & Ernilasari, 2018). The extracts, fractions, and isolates of M. ferrea have been evaluated for various KEYWORDS Bioactivities Extract Fractions Mesua ferrea L. FTIR fingerprint

Research paper thumbnail of Aktivitas Immunodulator Ekstrak Etanol Umbi Bawang Merah (Allium cepa L.) terhadap Respon Imun Non Spesifik pada Mencit Jantan Galur BALB/C dengan Metode Carbon Clearance

Biosfera, 2017

The content of flavonoids on onion useful to maintain endurance. This research aims to know the a... more The content of flavonoids on onion useful to maintain endurance. This research aims to know the activity of exstract immunodulator of onion bulb ethanol (Allium cepa L) on phagocytic activity in male mice balb/c with varian dose and campored immunodulator activity with positive control. This study is an experimental reseach with Completely Randomized Design (CRD). Preparation of extract ethanol onion roots done by maceration using 96% ethanol for 9 hours. The test performed on samples which consist of 25 mice with carbon clearance method, devided into 5 groups, which are : K – (without treatment), k+ (with imboos 13,3 ml/Kg Bw), P1 (dose 12%), P2 (dose 24%), and P3 (dose 48%). The test performed for 5 days, and on the 7th day injected carbon (pelicans) as much 0,1 ml/ 10 gr Weight intravenousty, their blood taken before carbon injection (minute 0) and minute 4, 8, 12, and 16. After their transmittance measure using spectrophotometer at 600,5 nm wavelength. This research shows that e...