chris elson - Academia.edu (original) (raw)

Papers by chris elson

Research paper thumbnail of The role of oil and agalactosyl IgG in the induction of arthritis in rodent models

European journal of …, Jan 1, 1991

The proportion of agalactosyl IgG [Gal(O)] is raised in human rheumatoid arthritis and tuberculos... more The proportion of agalactosyl IgG [Gal(O)] is raised in human rheumatoid arthritis and tuberculosis. We report here that injection of pristane into the peritoneal cavities of mice on days0 and 50, which is known to induce plasmacytomas and arthritis, also induced a rise in the ...

Research paper thumbnail of Antigenicity of rat erythrocyte glycophorins

Immunology Letters, 1988

The relationship between rat red blood cell (RBC) glycophorins and the antigens recognised by ant... more The relationship between rat red blood cell (RBC) glycophorins and the antigens recognised by anti-rat RBC antibodies was examined. Initially, murine monoclonal antibodies specific for surface epitopes on whole rat RBCs were tested for their reactivity with RBC membranes on Western blots and two were found which reacted with blotted antigens. These antibodies recognised two bands corresponding to the major PAS-stainable bands of rat RBC membranes (i.e., the glycophorins) and a number of minor bands, thus demonstrating that the bands are antigenically related. This band-pattern was remarkably similar to that obtained with mouse anti-rat RBC serum. Digestion with neuraminidase altered the electrophoretic mobility of most of the bands, providing additional evidence that they are sialoglycoproteins, although sialic acid was shown not to contribute to their antigenicity. The glycophorin nature of the major antigens was verified by reelectrophoresis and blotting of bands excised from SDS gels, which showed that they were interconvertible monomeric and dimeric forms of the same polypeptide chain. It is suggested that rat RBC glycophorins are a related family of sialoglycoproteins with the high molecular weight members being formed by dimerization of five lower molecular weight polypeptide chains in various combinations.

Research paper thumbnail of Handbook of Animal Models for the Rheumatoid Diseases

Annals of The Rheumatic Diseases, 1989

Research paper thumbnail of Interleukin 1 alpha and beta production by cells isolated from membranes around aseptically loose total joint replacements

Annals of The Rheumatic Diseases, 1992

Aseptic loosening of joint prostheses is accompanied by local osteolysis. To determine whether lo... more Aseptic loosening of joint prostheses is accompanied by local osteolysis. To determine whether local production of interleukin 1 might contribute to such lysis, the number of interleukin 1 secreting cells in the pseudosynovial membrane surrounding prostheses was measured. Interleukin la and 13 secreting cells were identified by ELISPOT, a sensitive cytokine secreting assay. The proportion of interleukin 113 secreting cells in pseudosynovial membrane was comparable with the proportion occurring in the synovium of patients with rheumatoid arthritis and higher than that in normal subjects and patients with osteoarthritis. The proportion of interleukin la producing celis was higher in pseudosynovial membrane than in diseased synovium. Overall, higher numbers of interleukin 113 than interleukin la secreting cells were detected. A correlation was observed between the number of cells in pseudosynovial membrane producing interleukin 13 and those producing interleukin la. When divided into area of origin, tissue samples from the femoral area contained a higher proportion of interleukin 1B producing cells than tissue in the acetabular or capsular regions, though due to variance within each group this difference did not reach significance.

Research paper thumbnail of The role of oil and agalactosyl IgG in the induction of arthritis in rodent models

European Journal of Immunology, 1991

The proportion of agalactosyl IgG [Gal(O)] is raised in human rheumatoid arthritis and tuberculos... more The proportion of agalactosyl IgG [Gal(O)] is raised in human rheumatoid arthritis and tuberculosis. We report here that injection of pristane into the peritoneal cavities of mice on days 0 and 50, which is known to induce plasmacytomas and arthritis, also induced a rise in the proportion of Gal(O), correlating with a simultaneous rise in the level of IgG antibody binding to the 65-kDa heat-shock protein of Mycobacterium bovis (hsp65). Arthritis developed in a proportion of those CBA/Igb mice with the highest percentage of Gal(O). Pretreatment with 50 μg of recombinant mycobacterial hsp65 intraperitoneal (i.p.) on day —10, or with 500 rad irradiation on day - 2 before the first of the two injections of pristane reduced the incidence of arthritis from 24% in control animals, to 5.3% and 0.4%, respectively. The reduced incidence of disease correlated with smaller rises in the % Gal(O) at 50–75 days, although levels at 150–200 days were not affected.The arthritogenic effect of oil was not confined to the pristane model, since a single i.p. injection of oil 21 days before immunizing DBA/1 mice with type II collagen reduced the mean day of onset of this arthritis, [which we have previously shown to correlate with raised % Gal(O)], from 38 to 15 days (p < 0.001). One interpretation is that an autoimmunogenic stimulus, given when % Gal (O) is raised, is more likely to evoke disease.Since oil granulomata are known to secrete interleukin 6, which has B cell-regulatory properties and is secreted by rheumatoid synovial cells, we tested sera from interleukin 6-transgenic mice, and found a strikingly raised percentage of Gal(O). We suggest, therefore, that the role of oil in the induction of arthritis is the dysregulation of cytokine release of which a raised percentage of Gal(O) may be a direct or indirect consequence, associated with an increased susceptibility to autoimmunogenic stimuli.

Research paper thumbnail of The role of oil and agalactosyl IgG in the induction of arthritis in rodent models

European journal of …, Jan 1, 1991

The proportion of agalactosyl IgG [Gal(O)] is raised in human rheumatoid arthritis and tuberculos... more The proportion of agalactosyl IgG [Gal(O)] is raised in human rheumatoid arthritis and tuberculosis. We report here that injection of pristane into the peritoneal cavities of mice on days0 and 50, which is known to induce plasmacytomas and arthritis, also induced a rise in the ...

Research paper thumbnail of Antigenicity of rat erythrocyte glycophorins

Immunology Letters, 1988

The relationship between rat red blood cell (RBC) glycophorins and the antigens recognised by ant... more The relationship between rat red blood cell (RBC) glycophorins and the antigens recognised by anti-rat RBC antibodies was examined. Initially, murine monoclonal antibodies specific for surface epitopes on whole rat RBCs were tested for their reactivity with RBC membranes on Western blots and two were found which reacted with blotted antigens. These antibodies recognised two bands corresponding to the major PAS-stainable bands of rat RBC membranes (i.e., the glycophorins) and a number of minor bands, thus demonstrating that the bands are antigenically related. This band-pattern was remarkably similar to that obtained with mouse anti-rat RBC serum. Digestion with neuraminidase altered the electrophoretic mobility of most of the bands, providing additional evidence that they are sialoglycoproteins, although sialic acid was shown not to contribute to their antigenicity. The glycophorin nature of the major antigens was verified by reelectrophoresis and blotting of bands excised from SDS gels, which showed that they were interconvertible monomeric and dimeric forms of the same polypeptide chain. It is suggested that rat RBC glycophorins are a related family of sialoglycoproteins with the high molecular weight members being formed by dimerization of five lower molecular weight polypeptide chains in various combinations.

Research paper thumbnail of Handbook of Animal Models for the Rheumatoid Diseases

Annals of The Rheumatic Diseases, 1989

Research paper thumbnail of Interleukin 1 alpha and beta production by cells isolated from membranes around aseptically loose total joint replacements

Annals of The Rheumatic Diseases, 1992

Aseptic loosening of joint prostheses is accompanied by local osteolysis. To determine whether lo... more Aseptic loosening of joint prostheses is accompanied by local osteolysis. To determine whether local production of interleukin 1 might contribute to such lysis, the number of interleukin 1 secreting cells in the pseudosynovial membrane surrounding prostheses was measured. Interleukin la and 13 secreting cells were identified by ELISPOT, a sensitive cytokine secreting assay. The proportion of interleukin 113 secreting cells in pseudosynovial membrane was comparable with the proportion occurring in the synovium of patients with rheumatoid arthritis and higher than that in normal subjects and patients with osteoarthritis. The proportion of interleukin la producing celis was higher in pseudosynovial membrane than in diseased synovium. Overall, higher numbers of interleukin 113 than interleukin la secreting cells were detected. A correlation was observed between the number of cells in pseudosynovial membrane producing interleukin 13 and those producing interleukin la. When divided into area of origin, tissue samples from the femoral area contained a higher proportion of interleukin 1B producing cells than tissue in the acetabular or capsular regions, though due to variance within each group this difference did not reach significance.

Research paper thumbnail of The role of oil and agalactosyl IgG in the induction of arthritis in rodent models

European Journal of Immunology, 1991

The proportion of agalactosyl IgG [Gal(O)] is raised in human rheumatoid arthritis and tuberculos... more The proportion of agalactosyl IgG [Gal(O)] is raised in human rheumatoid arthritis and tuberculosis. We report here that injection of pristane into the peritoneal cavities of mice on days 0 and 50, which is known to induce plasmacytomas and arthritis, also induced a rise in the proportion of Gal(O), correlating with a simultaneous rise in the level of IgG antibody binding to the 65-kDa heat-shock protein of Mycobacterium bovis (hsp65). Arthritis developed in a proportion of those CBA/Igb mice with the highest percentage of Gal(O). Pretreatment with 50 μg of recombinant mycobacterial hsp65 intraperitoneal (i.p.) on day —10, or with 500 rad irradiation on day - 2 before the first of the two injections of pristane reduced the incidence of arthritis from 24% in control animals, to 5.3% and 0.4%, respectively. The reduced incidence of disease correlated with smaller rises in the % Gal(O) at 50–75 days, although levels at 150–200 days were not affected.The arthritogenic effect of oil was not confined to the pristane model, since a single i.p. injection of oil 21 days before immunizing DBA/1 mice with type II collagen reduced the mean day of onset of this arthritis, [which we have previously shown to correlate with raised % Gal(O)], from 38 to 15 days (p < 0.001). One interpretation is that an autoimmunogenic stimulus, given when % Gal (O) is raised, is more likely to evoke disease.Since oil granulomata are known to secrete interleukin 6, which has B cell-regulatory properties and is secreted by rheumatoid synovial cells, we tested sera from interleukin 6-transgenic mice, and found a strikingly raised percentage of Gal(O). We suggest, therefore, that the role of oil in the induction of arthritis is the dysregulation of cytokine release of which a raised percentage of Gal(O) may be a direct or indirect consequence, associated with an increased susceptibility to autoimmunogenic stimuli.