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Papers by hanaa abdallah

BMC pediatrics, Feb 28, 2024

Background Neonates with intrauterine growth retardation (IUGR) may present with fatal complicati... more Background Neonates with intrauterine growth retardation (IUGR) may present with fatal complications and permanent serious consequences. Vitamin status may influence fetal development. In this study we assessed vitamin A, E and D concentrations in umbilical cord blood in newborns with IUGR. Methods Maternal data were obtained. Neonatal assessment included; age of gestation calculated from last menstrual period, Ultrasound (U/S), new Ballard, Apgar scores and anthropometric measurements including; Head circumference, length and weight. WHO growth percentile curves were used. Vitamin A, E and D in cord blood samples were measured by high performance liquid chromatography (HPLC) and ELISA consecutively. Results A total of 86 full term newborns were enrolled in this study, 42 (48.8%) with IUGR with gestational age (33.59 ± 1.20) week by U/S and 44 (51.2%) appropriate for gestational age neonates with gestational age (38.70 ± 1.50). Ballard and Apgar scores (p < 0.05) and Z scores for weight, length and head circumference (p < 0.001) at birth were significantly lower in neonates with Intrauterine growth retardation (IUGR) than appropriate for gestational age (AGA) neonates. The levels of Vitamin A, E and D were significantly lower in the IUGR group than the AGA (p < 0.05) for all. Significant positive correlations of weight with vitamin A, and E cord blood levels were found (p < 0.05), while length was significantly positively correlated only with vitamin A (p < 0.05). Head circumference showed significant positive correlations with the three vitamins (p < 0.05) for all. Conclusion Neonates with IUGR had significantly lower levels of Vitamin A, E and D than AGA neonates. Significant positive correlations of weight with vitamin A, and E cord blood levels was detected, while neonatal length was associated only with vitamin A level. The present study highlights the significance of nutritional policies for inhibiting deficiency of these vitamins during pregnancy and childhood.

Egyptian Journal of Chemistry, Feb 5, 2024

Open Access Macedonian Journal of Medical Sciences, Apr 6, 2018

BACKGROUND: Myeloperoxidase (MPO) is an enzyme involved in the pathogenesis of several diseases. ... more BACKGROUND: Myeloperoxidase (MPO) is an enzyme involved in the pathogenesis of several diseases. AIM: The current study aimed to investigate serum MPO levels in obese Egyptian women and assess its relation with insulin resistance (IR) and other biochemical risk parameters. METHODS: The study included 80 obese women and 50 age-and-sex-matched healthy controls. Insulin resistance (IR) was evaluated by the Homeostasis Model Assessment-Insulin Resistance (HOMA-IR). Serum MPO, fasting glucose, insulin and blood lipids and anthropometry were measured. Obese cases were divided into three groups based on MPO tertiles. ROC analysis was performed to obtain the optimal cutoff values of MPO to predicate IR in obese women. RESULTS: The mean serum MPO was significantly higher in obese cases than controls. Cases in the highest MPO tertile had higher HOMA-IR, blood lipids and pressure levels compared with those in the lower tertile. The cutoff point of MPO was > 87.8 (ng/mL) and area under curves was 0.82 (p < 0.01) for diagnosis of IR. MPO levels were higher in obese Egyptian women than healthy controls. CONCLUSION: Elevation of MPO was associated with abnormal metabolic parameters. MPO might be used as an earlier biomarker for IR and metabolic disturbance in obese women.

BMC pediatrics, Feb 24, 2024

Background Using Zonulin and Copeptin as potential obesity markers in children, hasn't yet been f... more Background Using Zonulin and Copeptin as potential obesity markers in children, hasn't yet been focused. Aim To evaluate the association between serum levels of both Zonulin and Copeptin with the obesity markers, and to assess their role as metabolic disturbance predictors in obese children. Methods A case-control study comprised 111 Egyptian children (45 males and 66 females); aged 6-10 years to avoid the effect of puberty (prepubertal). They were classified according to their body mass index (BMI) percentiles into: 72 obese (BMI ≥ 95th), and 39 control ones (BMI > 15th-<85th), based on the Egyptian Growth Charts for children and adolescents. Anthropometric parameters and blood pressure were measured, and body composition analysis, lipid profile, Zonulin, and Copeptin levels were assessed. Results The obese group showed a significantly higher value of Copeptin and a lower value of Zonulin than the control one Also, the obese group showed significant negative correlations between Zonulin and both anthropometric obesity markers and body composition, whereas Copeptin showed significant positive ones. Moreover, significant positive correlations were found between Copeptin and both body weight and fat distribution. Insignificant correlations were observed between both serum Zonulin and Copeptin levels and blood pressure and lipid profile. Conclusion Zonulin and Copeptin cannot be used as metabolic disturbance predictors, among Egyptian children, as they were insignificantly correlated with lipid profile or blood pressure.

Diabetology & Metabolic Syndrome, Mar 21, 2023

Background Type 1 diabetes mellitus (T1DM) patients are at an increased risk for non-alcoholic fa... more Background Type 1 diabetes mellitus (T1DM) patients are at an increased risk for non-alcoholic fatty liver disease (NAFLD). This study aimed to evaluate the clinical criteria associated with the diagnosis of Non-Alcoholic Fatty Liver Disease (NAFLD) among T1DM Egyptian children and adolescents. Methods 74 T1DM patients aged 8-18 year were enrolled in this cross sectional study. Assessments of Clinical status, anthropometric measures, lipid profile, glycated haemoglobin (HbA1c) and liver enzymes were done. Abdominal Ultrasound evaluation of hepatic steatosis was done. Accordingly, patients were divided into two groups (NAFLD and normal liver group) and compared together. Assessment of liver fibrosis using acoustic radiation force impulse elastography (ARFI) was done. Statistical analysis included; independent t-test, Chi square and Fisher's Exact, Pearson and Spearman tests and Logistic regression models for factors associated with fatty liver were used when appropriate. Results In this study; 74 patients were enrolled; 37 males (50%) and 37 females with mean age 14.3 ± 3.0 year. The mean insulin dose was 1.1 ± 0.4 U/kg and mean disease duration was 6.3 ± 3.0 year. NAFLD was detected in 46 cases while 28 cases had normal liver as diagnosed by abdominal ultrasound. Cases with NAFLD had statistically significant higher BMI-Z scores, waist/hip, waist/height and sum of skin fold thicknesses compared to those with normal liver (P < 0.05). The mean value of HbA1c % was significantly higher in NAFLD group (P = 0.003). Total cholesterol, triglycerides and LDL serum levels were significantly elevated (p < 0.05), while the HDL level was significantly lower in NAFLD cases (p = 0.001). Although, serum levels of liver enzymes; ALT and AST were significantly higher among cases with NAFLD than in normal liver group (p < 0.05), their means were within normal. Using the ARFI elastography; NAFLD cases exhibited significant fibrosis (F2, 3 and 4). BMI, patient age and female gender were among risk factors for NAFLD. Conclusions NAFLD represents a serious consequence in type 1 diabetic children and adolescents that deserves attention especially with poor glycemic control. NAFLD has the potential to evolve to fibrosis. This study demonstrated a very high prevalence of NAFLD in T1D children and adolescents using US which was (62.2%) with the

Background Type 1 diabetes mellitus patients are at an increased risk for non-alcoholic fatty liv... more Background Type 1 diabetes mellitus patients are at an increased risk for non-alcoholic fatty liver disease (NAFLD). This study aimed to evaluate the clinical criteria associated with the diagnosis of Non Alcoholic Fatty Liver Disease (NAFLD) among Egyptian children and adolescents with type 1 Diabetes. Methods 74 T1DM patients aged 8–18 y were enrolled in this cross sectional study. Clinical examination and anthropometric assessment were done. Assessments of lipid profile, glycated haemoglobin (HbA1c) and liver enzymes were done. Abdominal Ultrasound evaluation of hepatic steatosis was done and accordingly, patients were divided into two groups (NAFLD & normal liver group) and compared together. Assessment of liver fibrosis using acoustic radiation force impulse elastography (ARFI) was done. Results in this study; 37 male (50%) & 37 females with mean age 14.3 ± 3.0 y. The mean insulin dose was 1.1 ± 0.4 U/kg & mean disease duration was 6.3 ± 3.0 y. NAFLD was detected in 46 cases wh...

Background Free radicals' excessive production contributes to increasing the burden of oxidative ... more Background Free radicals' excessive production contributes to increasing the burden of oxidative stress in critically ill patients. This could be involved in the pathogenesis of tissue damage and systemic dysfunction. In this study we aimed to assess the oxidative stress status through evaluation of oxidants and antioxidants levels in critically ill pediatric patients. Methods This study included 25 pediatric ICU critically ill patients; and 25 healthy age and sex matched controls. Patients were subjected to detailed medical history and clinical examination. The degree of critical illness was assessed according to qSOFA score. Laboratory investigations included complete blood count, blood culture, serum malondialdehyde (MDA) as an index of lipid peroxidation, serum total antioxidant capacity (TAC) and paraoxonase-1 serum level as an index of antioxidants level. The comparisons were done using Independent t-test, Mann-Whitney test and One Way ANOVA. The correlations were done by Spearman correlation coe cients. Receiver operating characteristic curve (ROC) was used to detect the predictive values and area under the curve (AUC) of the studied markers Results statistically signi cant elevation in the level of serum MDA and TAC were detected in patients than controls (p < 0.001) for each, and decrease in serum paraoxonase-1 in cases than the controls (p < 0.001). TAC was signi cantly increased in patients with septic shock (p < 0.05). Positive signi cant correlation was found between MDA and AST (p < 0.05), TAC and AST (p < 0.01) and TAC and INR (p < 0.05). Serum MDA predicts oxidative damage with sensitivity of 80%, speci city of 68%, serum paroxonase-1 with sensitivity of 80%, speci city of 68% and TAC sensitivity of 96%, speci city of 68% Conclusion Serum malondialdehyde and paraoxonase-1 can be used as a potential biomarkers for oxidative damage of critical illness in children with good sensitivity but low speci city, while TAC can also be used as a predictor for severity of illness in children. Therefore, change of the oxidative stress and anti-oxidant status could be a possible goal for therapy in critical illness. Background: Critical illness can be accompanied by dysfunction of major organs and systems. Sepsis could either be the cause of critical illness or complicates its course (Makris et al., 2018) [1]. Sepsis is a common clinical

Egyptian Journal of Medical Human Genetics, 2022

Background Down syndrome (DS) is characterized by variable degrees of intellectual disability (ID... more Background Down syndrome (DS) is characterized by variable degrees of intellectual disability (ID). The coronavirus disease-2019 (COVID-19) lockdown prevented children with DS from reaching their rehabilitation facilities. This could have led to deterioration of their abilities and mental health hazards. The aim of this cohort study was to investigate frequency of COVID-19, the influence of COVID-19 pandemic on health, and some abilities of children with DS, and to explore factors that could have governed receiving home-based training during the lockdown. A survey of 150 individuals with Down syndrome was answered by their caregivers. Additionally, 135 participants were subjected to assessment of cognitive, language, and motor abilities using Portage program. They were divided into 2 groups: group I who received online therapy sessions during the lockdown and group II who did not receive sessions. Logistic regression was used to determine the factors which influenced getting home-ba...

Meta Gene, 2018

Abstract Familial Mediterranean Fever (FMF) is an autosomal recessive disease characterized by re... more Abstract Familial Mediterranean Fever (FMF) is an autosomal recessive disease characterized by recurrent fever and inflammatory attacks. The aim of our study is to evaluate the growth parameters in Egyptian children with FMF and to investigate vitamin D status (serum 25-(OH) D) and some oxidative stress biomarkers during the attack free period in relation to the disease severity. Cases were classified into mild and moderate according to disease severity. Fifty Egyptian children with FMF (25 males and 25 females) and 35 age and sex-matched healthy controls were enrolled in this study. Serum paraoxonase1, malondialdehyde and Serum 25-(OH) D were estimated. Z-scores of weight, height and body mass index were calculated. Of the 50 patients with FMF, 20 (40%) had mild degree of severity and 30 (60%) with moderate degree of severity. The homozygous genotypes mutations of M694I and M694I + M680I were the most frequent (52% and 26%, respectively). Normal growth pattern was detected in both sexes. SerumPON1 and 25-(OH) D were significantly lower in FMF patients than the control group. On the other hand, during the attack free periods serum level of MDA was significantly higher in the moderate group compared to the mild group while PON1 was significantly lower in moderate group. The study suggests persistence of oxidative stress in FMF children during the attack free period. Vitamin D was significantly low in FMF patients. The study recommends the fortification with vitamin D and antioxidant parameters in the FMF patients.

Children with chromosomal disorders are at greater risk for cardiovascular comorbidities. Dyslipi... more Children with chromosomal disorders are at greater risk for cardiovascular comorbidities. Dyslipidemia and homocysteinemia have been identified to play a role in the pathogenesis of premature atherosclerosis. The current study aimed to explore the association of serum homocysteine level in children with numerical chromosomal disorders (Klinefelter syndrome, Down syndrome, and Turner syndrome) and explore its relation to dyslipidemia among such children. This case control study included 60 children with numerical chromosomal disorders (18 Klinefelter syndrome 22 Down syndrome and 20 Turner syndrome) diagnosed by cytogenetic analysis. In addition, 37 healthy normal weight children were included as control group. Anthropometric assessment was done for all groups including weight, height and body mass index was calculated. Serum level of homocysteine, cholesterol, triglycerides, high density lipoprotein and low-density lipoprotein were measured for all included children. Serum homocyste...

This cross-sectional study was conducted on 350, unrelated young women, aged between 20 and 30 ye... more This cross-sectional study was conducted on 350, unrelated young women, aged between 20 and 30 years. They are assembled according to WHO classification into three groups; normal weight (n=100), overweight (n=120) and obese (n=130). Interleukin-6 (IL-6) and C-reactive protein (CRP) were measured using standard methods and ELISA (R&D Systems. Insulin resistance was detected by the Homeostasis Model Assessment of Insulin Resistance (HOMA-IR). Anthropometric measures of adiposity showed significant positive correlations with inflammatory biomarkers (IL-6 and CRP) and inverse correlations with serum adiponectin levels (p<0.05). Obese and overweight women had significant lower serum adiponectin concentrations than normal weight women. In addition, significant negative correlation between serum adiponectin levels and inflammatory biomarkers was observed. Multivariate logistic regression analysis shows that increased WHR, WC and elevated blood pressure level are predictor’s risk factors...

Egyptian Pediatric Association Gazette, 2021

Familial Mediterranean fever (FMF) is autosomal recessive chronic disease represents by recurring... more Familial Mediterranean fever (FMF) is autosomal recessive chronic disease represents by recurring attacks of polyserositis, fever, and joint pain. Vitamin D deficiency in FMF children has been recently mentioned in literature and linked to delayed physical growth. Osteoporosis in FMF patients can be linked to low levels of vitamin D, too. Osteoprotegerin (OPG) might be used as an indicator for osteoporosis. Therefore, this work aimed to investigate the impact of vitamin D administration on clinical status, BMI, and bone mineral density represented by alterations in the OPG serum levels in a group of Egyptian children with FMF. This was a prospective longitudinal study carried out on 33 children, aged 4–16 years, with FMF cases. Patients were on colchicine 0.5–2 mg/day and received vitamin D3 oral drops 2800 IU/ml; each drop contains 100 IU in a dose of 600 IU/day for 6 months. The effect of vitamin D administration was evaluated clinically, anthropometrically and by assessment of se...

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH

EXCLI journal, 2017

Obesity is one of the diagnostic criteria of metabolic syndrome (MS). It is correlated with insul... more Obesity is one of the diagnostic criteria of metabolic syndrome (MS). It is correlated with insulin resistance (IR) and high vascular risk as well. Advanced glycation end products (AGEs) and their receptor (RAGE) play an important role in abnormal metabolic components in obese women. This study aimed to explore the serum levels of sRAGE in Egyptian obese women and compare with healthy non-obese controls and investigate the relationship between serum sRAGE, metabolic parameters, and obesity complications. The soluble form of receptor for advanced glycation end products (sRAGE), anthropometry, metabolic and biochemical biomarkers were measured in 100 obese women and 100 age-matched healthy control non-obese women. The homeostasis model assessment estimate of insulin resistance (HOMA-IR) has been determined from serum insulin and glucose values. Serum sRAGE levels were significantly lower in obese cases than controls and inversely correlated with obesity and metabolic parameters. Resul...

Open Access Macedonian Journal of Medical Sciences, 2016

AIM: To assess the associations between the body adiposity indices and risk of metabolic syndrome... more AIM: To assess the associations between the body adiposity indices and risk of metabolic syndrome (MS) and its components in Egyptian women and to evaluate their predictive power.MATERIALS AND METHODS: This was a cross-sectional analysis performed on 180 Egyptian women aged between 25-35 years. They were 90 women with MS diagnosed by International Diabetes Federation (IDF) and 90 healthy age matched controls. Body adiposity index (BAI), body mass index (BMI), waist to hip ratio (WHR) and waist to height ratio (WHtR) were calculated and serum samples were analyzed for metabolic parameters. Receiver operating characteristic curves (ROC) was used to determine the discriminatory capacity of BAI, WHR WHtR and BMI for MS.RESULTS: Area under the curve (AUC) was highest for BIA, followed by WHR, WHtR and then BMI. All adiposity indices were significantly correlated with metabolic components and BAI had the highest correlation coefficients compared to other indices.CONCLUSION: BAI is a pract...

Egyptian Pediatric Association Gazette, 2014

Introduction: Oxidative stress is the imbalance between pro-oxidants and antioxidants resulting i... more Introduction: Oxidative stress is the imbalance between pro-oxidants and antioxidants resulting in irreversible cell damage. Objective: To assess the oxidative stress status in a sample of Egyptian malnourished stunted children and investigate the relations between oxidative stress markers and anthropometric measurements. Patients and methods: This cross sectional descriptive analytical study was carried out on 50 malnourished stunted children (28 males and 22 females), aged from 6-9 years and 50 healthy age and sex-matched controls. Blood oxidative stress biomarkers including catalase (CAT), superoxide dismutase (SOD) malondialdehyde (MDA), plasma glutathione (GSH), total plasma proteins, total anti-oxidant capacity (TAC), copper (Cu), zinc (Zn), and vitamin C were measured in patients and controls. Socioeconomic status was assessed for patients. Body weight and height were measured and body mass index (BMI) was calculated. Patients were classified according to their height for age Z-scores (HAZ) into moderate and severe stunted. Results: Nutritionally stunted children showed significantly lower levels of the blood oxidative stress biomarkers including, CAT, SOD, plasma GSH, total plasma proteins, Cu, Zn and vitamin C and significantly higher levels of MDA compared with controls (p < 0.001). There was significant difference in plasma levels of Vitamin C and Zn between patients with different social levels. No significant relationships were found between the degree of stunting and oxidative markers. Conclusions: Nutritionally stunted children had an increased oxidative stress and decreased antioxidant defense system compared with healthy controls. Oxidative stress, malnutrition and low social level might play an important role in the pathogenesis of stunting.

International Journal of Cardiology, 2011