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Papers by humaira binte asad

Research paper thumbnail of Vitamin D deficiency and the vitamin D receptor (VDR) gene polymorphism rs2228570 (FokI) are associated with an increased susceptibility to hypertension among the Bangladeshi population

PloS one, Mar 14, 2024

Vitamin D receptor (VDR) gene is implicated in hypertension vulnerability due to its role in regu... more Vitamin D receptor (VDR) gene is implicated in hypertension vulnerability due to its role in regulating the renin-angiotensin system (RAS) and blood pressure. In this case-control study, a carefully selected cohort of 111 hypertensive individuals and 100 healthy controls underwent serum analysis using HPLC to measure 25-hydroxy vitamin D levels. Polymorphic variations in the VDR gene were detected and characterized using the PCR-RFLP method. At first, lower 25-hydroxy vitamin D levels were observed in hypertensive individuals compared to controls (p<0.001). The genotype frequency of the VDR gene TaqI showed no significant difference between cases and controls (p>0.05). Similarly, no significant difference was found in the VDR gene BsmI genotype frequency between hypertensive patients and controls (p>0.05). However, a statistically significant distinction was observed in the VDR gene FokI genotype frequency between cases and controls (p<0.01). The odds ratios for FokI genotypes (CC, CT, TT, and CT+TT) were 1.0, 0.590, 1.566, and 0.963, respectively. Furthermore, serum 25-hydroxy vitamin D levels were significantly higher in control subjects compared to hypertensive patients across all genotypes of VDR (p<0.001). Hypertensive patients, excluding those with the FokI VDR gene CC genotype, exhibited significantly higher systolic blood pressure levels compared to the control group (p<0.05). Similarly, hypertensive subjects displayed elevated diastolic blood pressure levels compared to the control group (p<0.001). Overall, the results suggest the presence of a potential inverse correlation between serum 25-hydroxy vitamin D levels and hypertension. The association analysis conducted indicated that there is no significant association between TaqI

Research paper thumbnail of Biochemical Parameters in Common Viral Hepatitis

Journal of medicine, 1970

Research paper thumbnail of Vitamin D deficiency and the vitamin D receptor (VDR) gene polymorphism rs2228570 (FokI) are associated with an increased susceptibility to hypertension among the Bangladeshi population

PLoS ONE 19(3): e0297138., 2024

Vitamin D receptor (VDR) gene is implicated in hypertension vulnerability due to its role in regu... more Vitamin D receptor (VDR) gene is implicated in hypertension vulnerability due to its role in regulating the renin-angiotensin system (RAS) and blood pressure. In this case-control study, a carefully selected cohort of 111 hypertensive individuals and 100 healthy controls underwent serum analysis using HPLC to measure 25-hydroxy vitamin D levels. Polymorphic variations in the VDR gene were detected and characterized using the PCR-RFLP method. At first, lower 25-hydroxy vitamin D levels were observed in hypertensive individuals compared to controls (p<0.001). The genotype frequency of the VDR gene TaqI showed no significant difference between cases and controls (p>0.05). Similarly, no significant difference was found in the VDR gene BsmI genotype frequency between hypertensive patients and controls (p>0.05). However, a statistically significant distinction was observed in the VDR gene FokI genotype frequency between cases and controls (p<0.01). The odds ratios for FokI genotypes (CC, CT, TT, and CT+TT) were 1.0, 0.590, 1.566, and 0.963, respectively. Furthermore, serum 25-hydroxy vitamin D levels were significantly higher in control subjects compared to hypertensive patients across all genotypes of VDR (p<0.001). Hypertensive patients, excluding those with the FokI VDR gene CC genotype, exhibited significantly higher systolic blood pressure levels compared to the control group (p<0.05). Similarly, hypertensive subjects displayed elevated diastolic blood pressure levels compared to the control group (p<0.001). Overall, the results suggest the presence of a potential inverse correlation between serum 25-hydroxy vitamin D levels and hypertension. The association analysis conducted indicated that there is no significant association between TaqI

Research paper thumbnail of Copper, Zinc levels of newly diagnosed type 2 Diabetic patients are better marker in RBC than in Plasma

International Journal of Diabetes & Metabolic Disorders, 2019

One of the greatest medical problems threatening the world today is Diabetes Mellitus. Aims of th... more One of the greatest medical problems threatening the world today is Diabetes Mellitus. Aims of this study were to compare plasma, RBC Cu and Zn between healthy and newly diagnosed type 2 diabetic patients, also association of hyperglycaemia, dyslipidaemia with plasma and RBC Cu, Zn. Study was carried out in departments of Biochemistry jointly with Endocrine Medicine of Bangabandhu Sheikh Mujib Medical University. Thirty three newly diagnosed type 2 DM, thirty one age and sex matched healthy controls were included. Both Cu and Zn were measured by atomic absorption spectrophotometer. The median value of plasma Cu in healthy controls was 942.00 ppb (ranging 846-1393.50 ppb), in newly diagnosed type 2 diabetic patients, 2739.00 ppb (ranging 1400- 5743.50ppb). Significantly higher level of plasma Cu was observed in cases (p

Research paper thumbnail of Spot morning urine protein creatinine ratio and 24 hour urinary total protein excretion rate

Mymensingh Medical Journal, 2007

Proteinuria helps to establish the diagnosis of most renal diseases and also to predict the outco... more Proteinuria helps to establish the diagnosis of most renal diseases and also to predict the outcome of such diseases. Proteinuria is biochemically represented by measuring the protein concentration in timed collection of 24 hour urine. But, 24-hour timed urine collection is time consuming, cumbersome and often unreliable due to collection errors and also results in undue delay on diagnostic process. An alternate approach avoiding arduous and inaccurate timed urine collection can be the measurement of protein creatinine ratio in spot morning urine. This study was aimed to evaluate whether the spot morning urine protein creatinine ratio can be a reliable alternative to 24-hour urinary total protein (UTP) estimation. The study was carried out in the department of Biochemistry, Bangabandhu Sheikh Mujib Medical University, Dhaka on 50 (fifty) non-diabetic Chronic Renal Disease (CRD) patients with an age ranging from 18 -70 years. The study subjects were grouped into mild, moderate and severe CRD on the basis of GFR. Urinary protein and creatinine concentrations were measured in spot morning urine samples and their ratios were calculated. Urinary protein measured in 24-hour timed collected urine samples gave the 24-hour UTP excretion rate. In our study, spot morning urine protein creatinine ratio significantly correlated with 24-hour UTP excretion rate in all CRD patients. Severe CRD patients gave significant positive correlation (p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05), whereas mild and moderate CRD patients gave very highly significant positive correlation (p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.001). Therefore, it may be suggested that protein creatinine ratio in spot morning urine can be accepted as a reliable and alternative to 24-hour UTP excretion rate in non-diabetic chronic renal disease patients. This simple and inexpensive procedure will thus simplify the way of establishing the severity of renal disease along with its prognosis.

Research paper thumbnail of Spot morning urine protein creatinine ratio and 24 hour urinary total protein excretion rate.

Mymensingh Medical Journal : MMJ, 01 Jul 2006, 15(2):146-149, 2006

Proteinuria helps to establish the diagnosis of most renal diseases and also to predict the outco... more Proteinuria helps to establish the diagnosis of most renal diseases and also to predict the outcome of such diseases. Proteinuria is biochemically represented by measuring the protein concentration in timed collection of 24 hour urine. But, 24-hour timed urine collection is time consuming, cumbersome and often unreliable due to collection errors and also results in undue delay on diagnostic process. An alternate approach avoiding arduous and inaccurate timed urine collection can be the measurement of protein creatinine ratio in spot morning urine. This study was aimed to evaluate whether the spot morning urine protein creatinine ratio can be a reliable alternative to 24-hour urinary total protein (UTP) estimation. The study was carried out in the department of Biochemistry, Bangabandhu Sheikh Mujib Medical University, Dhaka on 50 (fifty) non-diabetic Chronic Renal Disease (CRD) patients with an age ranging from 18 -70 years. The study subjects were grouped into mild, moderate and severe CRD on the basis of GFR. Urinary protein and creatinine concentrations were measured in spot morning urine samples and their ratios were calculated. Urinary protein measured in 24-hour timed collected urine samples gave the 24-hour UTP excretion rate. In our study, spot morning urine protein creatinine ratio significantly correlated with 24-hour UTP excretion rate in all CRD patients. Severe CRD patients gave significant positive correlation (p<0.05), whereas mild and moderate CRD patients gave very highly significant positive correlation (p<0.001). Therefore, it may be suggested that protein creatinine ratio in spot morning urine can be accepted as a reliable and alternative to 24-hour UTP excretion rate in non-diabetic chronic renal disease patients. This simple and inexpensive procedure will thus simplify the way of establishing the severity of renal disease along with its prognosis.

Research paper thumbnail of Fluorescent in Situ Hybridization

Bangladesh J of Medical Biochemistry, 2015

exception: in XNA nucleotides. The deoxyribose and ribose sugar groups of DNA and RNA have been r... more exception: in XNA nucleotides. The deoxyribose and ribose sugar groups of DNA and RNA have been replaced. Some of these replacement molecules contain four carbon atoms instead of the standard five. Others contain as many as seven carbons. The name of the backbone is then reflected in the name of the nucleic acid. Such synthetic 'XNA' created so far include HNA (1,5-anhydrohexitol nucleic acid), CeNA (cyclohexenyl nucleic acid), TNA (threose nucleic acid), ANA (arabino nucleic acid), FANA (2' Fluro-arabino nucleic acid), LNA (locked nucleic acid), GNA (glycol nucleic acid) and PNA (peptide nucleic acid) 3. These substitutions make XNAs functionally and structurally analogous to DNA and RNA.

Research paper thumbnail of Endocrinology Congress 2016: Association of fasting insulin with plasma, RBC micronutrients copper and zinc in newly diagnosed type 2 diabetic patients

Journal of Clinical Developmental Biology, 2017

Research paper thumbnail of Copper, Zinc levels of newly diagnosed type 2 Diabetic patients are better marker in RBC than in Plasma

www.opastonline.com, 2019

Research paper thumbnail of Biochemical Parameters in Common Viral Hepatitis

Journal of Medicine, 2010

Viral hepatitis is the inflammation of the liver caused by hepatitis viruses. The most common cau... more Viral hepatitis is the inflammation of the liver caused by hepatitis viruses. The most common causes of viral hepatitis are the five unrelated hepatotropic viruses Hepatitis A, Hepatitis B, Hepatitis C, Hepatitis D, and Hepatitis E. The aim of this study is to assess the biochemical parameters in viral hepatitis which varies with respect to the different types of viral hepatitis. Sex of the patient affected by Hepatitis A was almost similar in male and female, being 9 (45%) and 11 (55%) in respectively. But in contrast, more than eighty per cent (85%) Hepatitis-E affected population was male. Similar scenario was found in Hepatitis B And C infection (Male- 75%,67%, Female 25,35%). Mean value with standard deviation (±SD) of serum bilirubin level was highest in Hepatitis E (251 ± 125.19 ìmol/l). Value of serum ALT in hepatitis E was found to be 1794 U/l (highest), hepatitis B 1362 U/l hepatitis C are 135.45 U/L,. Serum aspartate aminotransferase (S.AST) is also raised in all types of...

Research paper thumbnail of A Partial Sketch of 2018 from Laboratory Services Department at National Institute of ENT

Biochemistry & Analytical Biochemistry, 2021

Laboratories play a crucial role in patient diagnosis, follow up, disease surveillance, control a... more Laboratories play a crucial role in patient diagnosis, follow up, disease surveillance, control and provide accurate health data for proper national health planning and resource mobilization. The objectives of the study were to assess quantity and distribution of patients achieving laboratory services at a tertiary level hospital like National Institute of Ear Nose Throat. Because the patient distribution and quantification of particular tests guide us to plan and distribute our resources for the next fiscal year. Performance of the lab services ensure us the confidence of clients over quality services. A descriptive study was performed from 1st January to 31st December 2018 at Laboratory Services Department of NIENT, Dhaka, Bangladesh. All those patients attended the department to attain any particular service existing at that particular time were included in the study. Total patients received services were 9805 (of which males 4785,females 5003) and male:female ratio, 0.96. Only 8...

Research paper thumbnail of Vitamin D deficiency and the vitamin D receptor (VDR) gene polymorphism rs2228570 (FokI) are associated with an increased susceptibility to hypertension among the Bangladeshi population

PloS one, Mar 14, 2024

Vitamin D receptor (VDR) gene is implicated in hypertension vulnerability due to its role in regu... more Vitamin D receptor (VDR) gene is implicated in hypertension vulnerability due to its role in regulating the renin-angiotensin system (RAS) and blood pressure. In this case-control study, a carefully selected cohort of 111 hypertensive individuals and 100 healthy controls underwent serum analysis using HPLC to measure 25-hydroxy vitamin D levels. Polymorphic variations in the VDR gene were detected and characterized using the PCR-RFLP method. At first, lower 25-hydroxy vitamin D levels were observed in hypertensive individuals compared to controls (p<0.001). The genotype frequency of the VDR gene TaqI showed no significant difference between cases and controls (p>0.05). Similarly, no significant difference was found in the VDR gene BsmI genotype frequency between hypertensive patients and controls (p>0.05). However, a statistically significant distinction was observed in the VDR gene FokI genotype frequency between cases and controls (p<0.01). The odds ratios for FokI genotypes (CC, CT, TT, and CT+TT) were 1.0, 0.590, 1.566, and 0.963, respectively. Furthermore, serum 25-hydroxy vitamin D levels were significantly higher in control subjects compared to hypertensive patients across all genotypes of VDR (p<0.001). Hypertensive patients, excluding those with the FokI VDR gene CC genotype, exhibited significantly higher systolic blood pressure levels compared to the control group (p<0.05). Similarly, hypertensive subjects displayed elevated diastolic blood pressure levels compared to the control group (p<0.001). Overall, the results suggest the presence of a potential inverse correlation between serum 25-hydroxy vitamin D levels and hypertension. The association analysis conducted indicated that there is no significant association between TaqI

Research paper thumbnail of Biochemical Parameters in Common Viral Hepatitis

Journal of medicine, 1970

Research paper thumbnail of Vitamin D deficiency and the vitamin D receptor (VDR) gene polymorphism rs2228570 (FokI) are associated with an increased susceptibility to hypertension among the Bangladeshi population

PLoS ONE 19(3): e0297138., 2024

Vitamin D receptor (VDR) gene is implicated in hypertension vulnerability due to its role in regu... more Vitamin D receptor (VDR) gene is implicated in hypertension vulnerability due to its role in regulating the renin-angiotensin system (RAS) and blood pressure. In this case-control study, a carefully selected cohort of 111 hypertensive individuals and 100 healthy controls underwent serum analysis using HPLC to measure 25-hydroxy vitamin D levels. Polymorphic variations in the VDR gene were detected and characterized using the PCR-RFLP method. At first, lower 25-hydroxy vitamin D levels were observed in hypertensive individuals compared to controls (p<0.001). The genotype frequency of the VDR gene TaqI showed no significant difference between cases and controls (p>0.05). Similarly, no significant difference was found in the VDR gene BsmI genotype frequency between hypertensive patients and controls (p>0.05). However, a statistically significant distinction was observed in the VDR gene FokI genotype frequency between cases and controls (p<0.01). The odds ratios for FokI genotypes (CC, CT, TT, and CT+TT) were 1.0, 0.590, 1.566, and 0.963, respectively. Furthermore, serum 25-hydroxy vitamin D levels were significantly higher in control subjects compared to hypertensive patients across all genotypes of VDR (p<0.001). Hypertensive patients, excluding those with the FokI VDR gene CC genotype, exhibited significantly higher systolic blood pressure levels compared to the control group (p<0.05). Similarly, hypertensive subjects displayed elevated diastolic blood pressure levels compared to the control group (p<0.001). Overall, the results suggest the presence of a potential inverse correlation between serum 25-hydroxy vitamin D levels and hypertension. The association analysis conducted indicated that there is no significant association between TaqI

Research paper thumbnail of Copper, Zinc levels of newly diagnosed type 2 Diabetic patients are better marker in RBC than in Plasma

International Journal of Diabetes & Metabolic Disorders, 2019

One of the greatest medical problems threatening the world today is Diabetes Mellitus. Aims of th... more One of the greatest medical problems threatening the world today is Diabetes Mellitus. Aims of this study were to compare plasma, RBC Cu and Zn between healthy and newly diagnosed type 2 diabetic patients, also association of hyperglycaemia, dyslipidaemia with plasma and RBC Cu, Zn. Study was carried out in departments of Biochemistry jointly with Endocrine Medicine of Bangabandhu Sheikh Mujib Medical University. Thirty three newly diagnosed type 2 DM, thirty one age and sex matched healthy controls were included. Both Cu and Zn were measured by atomic absorption spectrophotometer. The median value of plasma Cu in healthy controls was 942.00 ppb (ranging 846-1393.50 ppb), in newly diagnosed type 2 diabetic patients, 2739.00 ppb (ranging 1400- 5743.50ppb). Significantly higher level of plasma Cu was observed in cases (p

Research paper thumbnail of Spot morning urine protein creatinine ratio and 24 hour urinary total protein excretion rate

Mymensingh Medical Journal, 2007

Proteinuria helps to establish the diagnosis of most renal diseases and also to predict the outco... more Proteinuria helps to establish the diagnosis of most renal diseases and also to predict the outcome of such diseases. Proteinuria is biochemically represented by measuring the protein concentration in timed collection of 24 hour urine. But, 24-hour timed urine collection is time consuming, cumbersome and often unreliable due to collection errors and also results in undue delay on diagnostic process. An alternate approach avoiding arduous and inaccurate timed urine collection can be the measurement of protein creatinine ratio in spot morning urine. This study was aimed to evaluate whether the spot morning urine protein creatinine ratio can be a reliable alternative to 24-hour urinary total protein (UTP) estimation. The study was carried out in the department of Biochemistry, Bangabandhu Sheikh Mujib Medical University, Dhaka on 50 (fifty) non-diabetic Chronic Renal Disease (CRD) patients with an age ranging from 18 -70 years. The study subjects were grouped into mild, moderate and severe CRD on the basis of GFR. Urinary protein and creatinine concentrations were measured in spot morning urine samples and their ratios were calculated. Urinary protein measured in 24-hour timed collected urine samples gave the 24-hour UTP excretion rate. In our study, spot morning urine protein creatinine ratio significantly correlated with 24-hour UTP excretion rate in all CRD patients. Severe CRD patients gave significant positive correlation (p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05), whereas mild and moderate CRD patients gave very highly significant positive correlation (p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.001). Therefore, it may be suggested that protein creatinine ratio in spot morning urine can be accepted as a reliable and alternative to 24-hour UTP excretion rate in non-diabetic chronic renal disease patients. This simple and inexpensive procedure will thus simplify the way of establishing the severity of renal disease along with its prognosis.

Research paper thumbnail of Spot morning urine protein creatinine ratio and 24 hour urinary total protein excretion rate.

Mymensingh Medical Journal : MMJ, 01 Jul 2006, 15(2):146-149, 2006

Proteinuria helps to establish the diagnosis of most renal diseases and also to predict the outco... more Proteinuria helps to establish the diagnosis of most renal diseases and also to predict the outcome of such diseases. Proteinuria is biochemically represented by measuring the protein concentration in timed collection of 24 hour urine. But, 24-hour timed urine collection is time consuming, cumbersome and often unreliable due to collection errors and also results in undue delay on diagnostic process. An alternate approach avoiding arduous and inaccurate timed urine collection can be the measurement of protein creatinine ratio in spot morning urine. This study was aimed to evaluate whether the spot morning urine protein creatinine ratio can be a reliable alternative to 24-hour urinary total protein (UTP) estimation. The study was carried out in the department of Biochemistry, Bangabandhu Sheikh Mujib Medical University, Dhaka on 50 (fifty) non-diabetic Chronic Renal Disease (CRD) patients with an age ranging from 18 -70 years. The study subjects were grouped into mild, moderate and severe CRD on the basis of GFR. Urinary protein and creatinine concentrations were measured in spot morning urine samples and their ratios were calculated. Urinary protein measured in 24-hour timed collected urine samples gave the 24-hour UTP excretion rate. In our study, spot morning urine protein creatinine ratio significantly correlated with 24-hour UTP excretion rate in all CRD patients. Severe CRD patients gave significant positive correlation (p<0.05), whereas mild and moderate CRD patients gave very highly significant positive correlation (p<0.001). Therefore, it may be suggested that protein creatinine ratio in spot morning urine can be accepted as a reliable and alternative to 24-hour UTP excretion rate in non-diabetic chronic renal disease patients. This simple and inexpensive procedure will thus simplify the way of establishing the severity of renal disease along with its prognosis.

Research paper thumbnail of Fluorescent in Situ Hybridization

Bangladesh J of Medical Biochemistry, 2015

exception: in XNA nucleotides. The deoxyribose and ribose sugar groups of DNA and RNA have been r... more exception: in XNA nucleotides. The deoxyribose and ribose sugar groups of DNA and RNA have been replaced. Some of these replacement molecules contain four carbon atoms instead of the standard five. Others contain as many as seven carbons. The name of the backbone is then reflected in the name of the nucleic acid. Such synthetic 'XNA' created so far include HNA (1,5-anhydrohexitol nucleic acid), CeNA (cyclohexenyl nucleic acid), TNA (threose nucleic acid), ANA (arabino nucleic acid), FANA (2' Fluro-arabino nucleic acid), LNA (locked nucleic acid), GNA (glycol nucleic acid) and PNA (peptide nucleic acid) 3. These substitutions make XNAs functionally and structurally analogous to DNA and RNA.

Research paper thumbnail of Endocrinology Congress 2016: Association of fasting insulin with plasma, RBC micronutrients copper and zinc in newly diagnosed type 2 diabetic patients

Journal of Clinical Developmental Biology, 2017

Research paper thumbnail of Copper, Zinc levels of newly diagnosed type 2 Diabetic patients are better marker in RBC than in Plasma

www.opastonline.com, 2019

Research paper thumbnail of Biochemical Parameters in Common Viral Hepatitis

Journal of Medicine, 2010

Viral hepatitis is the inflammation of the liver caused by hepatitis viruses. The most common cau... more Viral hepatitis is the inflammation of the liver caused by hepatitis viruses. The most common causes of viral hepatitis are the five unrelated hepatotropic viruses Hepatitis A, Hepatitis B, Hepatitis C, Hepatitis D, and Hepatitis E. The aim of this study is to assess the biochemical parameters in viral hepatitis which varies with respect to the different types of viral hepatitis. Sex of the patient affected by Hepatitis A was almost similar in male and female, being 9 (45%) and 11 (55%) in respectively. But in contrast, more than eighty per cent (85%) Hepatitis-E affected population was male. Similar scenario was found in Hepatitis B And C infection (Male- 75%,67%, Female 25,35%). Mean value with standard deviation (±SD) of serum bilirubin level was highest in Hepatitis E (251 ± 125.19 ìmol/l). Value of serum ALT in hepatitis E was found to be 1794 U/l (highest), hepatitis B 1362 U/l hepatitis C are 135.45 U/L,. Serum aspartate aminotransferase (S.AST) is also raised in all types of...

Research paper thumbnail of A Partial Sketch of 2018 from Laboratory Services Department at National Institute of ENT

Biochemistry & Analytical Biochemistry, 2021

Laboratories play a crucial role in patient diagnosis, follow up, disease surveillance, control a... more Laboratories play a crucial role in patient diagnosis, follow up, disease surveillance, control and provide accurate health data for proper national health planning and resource mobilization. The objectives of the study were to assess quantity and distribution of patients achieving laboratory services at a tertiary level hospital like National Institute of Ear Nose Throat. Because the patient distribution and quantification of particular tests guide us to plan and distribute our resources for the next fiscal year. Performance of the lab services ensure us the confidence of clients over quality services. A descriptive study was performed from 1st January to 31st December 2018 at Laboratory Services Department of NIENT, Dhaka, Bangladesh. All those patients attended the department to attain any particular service existing at that particular time were included in the study. Total patients received services were 9805 (of which males 4785,females 5003) and male:female ratio, 0.96. Only 8...