imam hasan - Academia.edu (original) (raw)
Papers by imam hasan
Anatomia, Histologia, Embryologia
The liver consists of parenchymal hepatocytes and non‐parenchymal cells. Non‐parenchymal cells, K... more The liver consists of parenchymal hepatocytes and non‐parenchymal cells. Non‐parenchymal cells, Kupffer cells, hepatic stellate cells and cholangiocytes have crucial roles in liver homeostasis and liver pathology. To establish baseline data, this study investigated immunohistochemically the distribution of non‐parenchymal cells in perivenular areas (PV), periportal areas (PP) and Glisson's sheath (GS) of adult rat liver. Liver tissues were collected from the left lateral lobe of rats. CD163‐positive macrophages were seen along the sinusoid of PV and PP areas, indicating Kupffer cells. Double immunofluorescence showed, Kupffer cells partly co‐expressed CD68 and MHC class II antigens in the liver. The numbers of Kupffer cells were significantly high in PP areas as compared with PV or GS areas. CD68‐positive exudative macrophages were highly localized in PP and GS areas and a comparatively low PV area. MHC class II‐positive dendritic cells (activated macrophages) were localized mainly in GS. Granzyme B‐positive NK cells were mainly localized in the Glisson's sheath. CD3‐positive T cells and CD20‐positive B cells were distributed along the sinusoids of the PP and PV areas of hepatic lobules. Vimentin and glial fibrillary acidic protein (GFAP)‐positive hepatic stellate cells were localized along sinusoids in the hepatic lobules of the liver. Cholangiocytes reacting to cytokeratin 19 were seen on interlobular bile ducts in Glisson's sheath of the liver. This study shows that heterogeneous macrophage populations, liver‐resident lymphocytes and hepatic stellate cells localized in PP and PV areas or GS areas of the liver with cells specific patterns.
Journal of Agriculture, Food and Environment, 2020
Effects of pesticide exposure on public health are monitored in developed countries, which is rar... more Effects of pesticide exposure on public health are monitored in developed countries, which is rare in the least developed countries like Bangladesh. Here, a surveillance study was conducted to investigate the farmer's knowledge and perception toward pesticide use and the impact of occupational exposure as well as an environmental hazard in six agro-based districts (Gazipur, Khulna, Manikganj, Naogaon, Mymensingh and Tangail) in Bangladesh. A questionnaire was designed for a face to face interview of the farmers (at least 100 farmers in each district), and the data was collected. The farmer during cultivations used pesticides as single, double or several times in a single crop cycle. Among different types of pesticides, insecticides were highly used (38%), whereas Imidacloprid, and Lamda cyhalothrin insecticide was top used by the farmers. Weedicides or herbicide, fungicides and rodenticides were used 11%, 48%, and 0.5%, respectively. The knowledge and perception of the farmers towards pesticide usage are minimum, whereas the environmental pollution aspect is deficient. The knowledge of using personal protective equipment is the least. Eighty five percent of farmers use pesticides by spraying in the field. Thirty nine percent of farmers suffered from sneezing, burning sensation on face, conjunctivitis, dizziness and headache during pesticide application, and 3% suffered from skin rash of long time exposed with pesticide. The exposure of pesticides is widespread in the professional life of a farmer in Bangladesh. So, it is essential to ensure different training and awareness building program for the farmers to cope the adverse situation.
The Iraqi Journal of Veterinary Medicine, 2021
Turkey bird is one of the popular poultry species which is reared primarily for meat production a... more Turkey bird is one of the popular poultry species which is reared primarily for meat production and considered as one of the major sources of animal protein. With such importance of this species, this study was designed to investigate the gross and histomorphology of the ovary and oviduct with especial emphasis on sperm-host glands of the turkey hen involving ten mature female turkeys (Meleagris gallopavo). The present study highlighted the distribution pattern of sperm-host glands (SHGs) in the oviduct of turkey hen that has a potential role in producing a fertile egg in poultry industries. The oviduct of turkey consists of the infundibulum, magnum, isthmus, uterus, and vagina which are sole distributors for making nutrition enriched egg. The tissue samples were collected from the ovary, different segments of the oviduct and especially uterovaginal junction (UVJ) and infundiomagnal junction of the oviduct. The ovaries and the oviducts were dissected and fixed in Bouins solution and...
Saudi Journal of Biological Sciences, 2021
The radiofrequency electromagnetic radiation emitted by smart phones on biological systems has wi... more The radiofrequency electromagnetic radiation emitted by smart phones on biological systems has wide media coverage and public concern in recent years. The aim of this study was to explore the effects of fourth-generation cell phone radiation exposure on hematological (Total leukocyte count, Total erythrocyte count, and hemoglobin %), biochemical (Serum creatinine) parameters, and histopathological changes in the kidney and testis of Swiss albino mice. A total of 30 male Swiss albino mice weighing 45-65 g was randomly divided into three groups (n = 10). The first group A was the control group, the second group B, was exposed to 40 minutes of mobile phone radiation daily, the third group C was exposed to 60 minutes of radiation daily from two 2400 Megahertz fourth-generation connected mobile phones for 60 days, respectively. The electromagnetic radiation frequency radiometer measured the frequency of electromagnetic radiation emitted from cell phones. The specific absorption rate was calculated as 0.087 W/kg. The control group was kept under similar conditions, but the electromagnetic field was not given for the same period. All the mice were sacrificed at the end of the experiment. The blood samples were collected for hematobiochemical study, and then kidney and testis tissues were collected for histopathological study. Results of the study showed that the body weight and total erythrocyte count values were significantly (p < 0.05) decreased while total leukocyte count, hemoglobin %, and serum creatinine values were significantly (p < 0.05) increased in both the radiation exposure groups relative to the control group. Histopathological observation showed the kidney of 60 minutes exposed mice interstitial inflammation that causes marked mononuclear cellular infiltration compared to the 40 minutes and control mice. Compared to control mice, histopathological examinations of testicular tissue from the exposed mice, showed irregular in shapes and non-uniform sizes and fewer spermatogenic cells layer that leads to the larger lumen in the seminiferous tubules. It is concluded that fourth-generation cell phone radiation exposure may affect blood hemostasis and inflammation of mice's kidney and testis tissue. Based on these studies, it is important to increase public consciousness of potential adverse effects of mobile phone radiofrequency electromagnetic radiation exposure.
March-2021, 2021
Background and Aim: Nowadays, toxic chemical contaminants in food are a major food safety problem... more Background and Aim: Nowadays, toxic chemical contaminants in food are a major food safety problem in Bangladesh. Among toxic food contaminants, formalin is used to preserve fruit, vegetables, and fish, where urea is used for the whitening of rice and puffed rice. The purpose of this study was to determine the biochemical and histopathological effects on the liver of young and adult pigeons after exposure to formalin and urea contaminated feed. Materials and Methods: A total of 15 young and 15 adult pigeons were divided into control group, formaldehyde exposed group (2.5 mL formalin/kg feed), and urea exposed (1 g/kg feed) group. Each group consisted of five pigeons. After the experimentation procedures, the blood samples were collected for biochemical study, and the liver tissue was collected for histomorphological study. The statistical analysis was performed using the Student's t-test, and p<0.05 was considered as statistically significant. Results: The aspartate transamina...
Journal of Advanced Biotechnology and Experimental Therapeutics, 2020
Formaldehyde (FA), a ubiquitous environmental pollutant, has long been suspected to possess repro... more Formaldehyde (FA), a ubiquitous environmental pollutant, has long been suspected to possess reproductive toxicity. Here, we investigated the histopathological alteration of male gonads following exposure to FA-contaminated feed (40% aqueous solution of FA; 2.5 ml formalin/kg feed) in pigeons for 7 days. The mean body weights were not changed significantly in FA-contaminated feed exposed pigeons compared with control pigeons. The hemoglobin concentration was significantly decreased and serum enzyme aspartate transaminase (AST) was significantly increased in FA-exposed pigeons in comparison with control pigeons. Histologically, the structural components of the testes are the seminiferous tubules and interstitial tissues, which are surrounded by a connective tissue capsule. In control pigeons, the size and shape of seminiferous tubules were normal with a regular arrangement of spermatogenic cells. In FA-exposed pigeons, the testicular capsule was thickened and degeneration of spermatogenic cells in the seminiferous tubules was observed. The number of spermatogenic cells was significantly decreased in the seminiferous tubules of FA-exposed pigeons in comparison with control pigeons, indicating that the low exposure of FA affects the spermatogenic cells' populations in male birds. The present results suggested that FA might cause infertility in birds.
Asian Journal of Medical and Biological Research, 2019
The objective of this study was to prepare the skeletons of duck to enhance veterinary anatomy te... more The objective of this study was to prepare the skeletons of duck to enhance veterinary anatomy teaching. Five aged ducks were used in this experiment, which was collected from the Bangladesh Agricultural University poultry farm. The ducks were skinned and different segments of the body were dissected with the help of a scalpel. Then the bones were wrapped using merkin clothes, heated in 5% solution of soda water for 1 hour, and kept in that solution for 7 days for decomposing the muscles, tendon and ligaments. For proper cleaning, the bones were dipped into 10% bleaching water for 2 hours. After complete sundry, the bones were articulated using super glue for rising into a skeleton. The vertebral column was aligned with 25-gauge steel wire was inserted into the vertebral foramen. Finally, the skeletons were fixed on a wooden stand. Therefore, the skeletons were preserved and used for demonstration of osteology, and avian anatomy. This duck skeleton will be used to study avian morpho...
Bangladesh Journal of Veterinary Medicine, 2017
This study was designed to determine the shiga toxin producing genes and to investigate antibioti... more This study was designed to determine the shiga toxin producing genes and to investigate antibiotic sensitivity or resistant patterns of the <em>Escherichia coli</em> isolated from diarrheic children at Mymensingh Medical College Hospital, Bangladesh. A total of 83 stool samples were collected and screened for the detection of <em>E. coli…
British Journal of Medicine and Medical Research, 2017
Aims: This study was conducted to determine virulent genes in Escherichia coli prevalent in duck ... more Aims: This study was conducted to determine virulent genes in Escherichia coli prevalent in duck population by multiplex Polymerase Chain Reaction. Methodology: A total of 60 cloacal swab samples were collected from two duck farms of Bangladesh Agricultural University and Shamvuganj. Initially the samples were screened for the detection of E. coli on the basis of cultural, staining and biochemical properties, followed by molecular detection of E. coli using genus specific primers to amplify 16s RNA. Results: According to the results, out of 60 samples, 26 (43.33%) were confirmed to be E. coli positive. Among the E. coli positive samples, 12 (46.15%) samples were found positive for Stx-1 and 11 for Stx-2. Among 26, 11 (42.31%) samples possess both Stx-1 and Stx-2 genes, whereas Original Research Article
Asian Pacific journal of tropical biomedicine, 2014
To analyze in vivo neuro-pharmacological effects of Alpinia nigra as anxiety is a particular form... more To analyze in vivo neuro-pharmacological effects of Alpinia nigra as anxiety is a particular form of behavioral inhibition that occurs in response to novel environmental events. In present study, the extract of Alpinia nigra was evaluated for its central nervous system depressant effect using mice behavioral models, such as hole cross, open field and thiopental sodium induced sleeping time tests for its sedative properties and an elevated plus-maze test for its anxiolytic potential, respectively. In anxiolytic study, the extract displayed increased percentage of entry into open arm at the dose of 400 and 200 mg/kg. The extract produced a significant (P<0.01) increase in sleeping duration and reduction of onset of sleep compared to sodium thiopental at both doses (200 and 400 mg/kg). The extract (200 and 400 mg/kg) also showed a dose-dependent suppression of motor activity and exploratory activity of the mice in both open field and hole cross test. This study demonstrates that the...
Saudi Journal of Biological Sciences, 2021
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Anatomia, Histologia, Embryologia
The liver consists of parenchymal hepatocytes and non‐parenchymal cells. Non‐parenchymal cells, K... more The liver consists of parenchymal hepatocytes and non‐parenchymal cells. Non‐parenchymal cells, Kupffer cells, hepatic stellate cells and cholangiocytes have crucial roles in liver homeostasis and liver pathology. To establish baseline data, this study investigated immunohistochemically the distribution of non‐parenchymal cells in perivenular areas (PV), periportal areas (PP) and Glisson's sheath (GS) of adult rat liver. Liver tissues were collected from the left lateral lobe of rats. CD163‐positive macrophages were seen along the sinusoid of PV and PP areas, indicating Kupffer cells. Double immunofluorescence showed, Kupffer cells partly co‐expressed CD68 and MHC class II antigens in the liver. The numbers of Kupffer cells were significantly high in PP areas as compared with PV or GS areas. CD68‐positive exudative macrophages were highly localized in PP and GS areas and a comparatively low PV area. MHC class II‐positive dendritic cells (activated macrophages) were localized mainly in GS. Granzyme B‐positive NK cells were mainly localized in the Glisson's sheath. CD3‐positive T cells and CD20‐positive B cells were distributed along the sinusoids of the PP and PV areas of hepatic lobules. Vimentin and glial fibrillary acidic protein (GFAP)‐positive hepatic stellate cells were localized along sinusoids in the hepatic lobules of the liver. Cholangiocytes reacting to cytokeratin 19 were seen on interlobular bile ducts in Glisson's sheath of the liver. This study shows that heterogeneous macrophage populations, liver‐resident lymphocytes and hepatic stellate cells localized in PP and PV areas or GS areas of the liver with cells specific patterns.
Journal of Agriculture, Food and Environment, 2020
Effects of pesticide exposure on public health are monitored in developed countries, which is rar... more Effects of pesticide exposure on public health are monitored in developed countries, which is rare in the least developed countries like Bangladesh. Here, a surveillance study was conducted to investigate the farmer's knowledge and perception toward pesticide use and the impact of occupational exposure as well as an environmental hazard in six agro-based districts (Gazipur, Khulna, Manikganj, Naogaon, Mymensingh and Tangail) in Bangladesh. A questionnaire was designed for a face to face interview of the farmers (at least 100 farmers in each district), and the data was collected. The farmer during cultivations used pesticides as single, double or several times in a single crop cycle. Among different types of pesticides, insecticides were highly used (38%), whereas Imidacloprid, and Lamda cyhalothrin insecticide was top used by the farmers. Weedicides or herbicide, fungicides and rodenticides were used 11%, 48%, and 0.5%, respectively. The knowledge and perception of the farmers towards pesticide usage are minimum, whereas the environmental pollution aspect is deficient. The knowledge of using personal protective equipment is the least. Eighty five percent of farmers use pesticides by spraying in the field. Thirty nine percent of farmers suffered from sneezing, burning sensation on face, conjunctivitis, dizziness and headache during pesticide application, and 3% suffered from skin rash of long time exposed with pesticide. The exposure of pesticides is widespread in the professional life of a farmer in Bangladesh. So, it is essential to ensure different training and awareness building program for the farmers to cope the adverse situation.
The Iraqi Journal of Veterinary Medicine, 2021
Turkey bird is one of the popular poultry species which is reared primarily for meat production a... more Turkey bird is one of the popular poultry species which is reared primarily for meat production and considered as one of the major sources of animal protein. With such importance of this species, this study was designed to investigate the gross and histomorphology of the ovary and oviduct with especial emphasis on sperm-host glands of the turkey hen involving ten mature female turkeys (Meleagris gallopavo). The present study highlighted the distribution pattern of sperm-host glands (SHGs) in the oviduct of turkey hen that has a potential role in producing a fertile egg in poultry industries. The oviduct of turkey consists of the infundibulum, magnum, isthmus, uterus, and vagina which are sole distributors for making nutrition enriched egg. The tissue samples were collected from the ovary, different segments of the oviduct and especially uterovaginal junction (UVJ) and infundiomagnal junction of the oviduct. The ovaries and the oviducts were dissected and fixed in Bouins solution and...
Saudi Journal of Biological Sciences, 2021
The radiofrequency electromagnetic radiation emitted by smart phones on biological systems has wi... more The radiofrequency electromagnetic radiation emitted by smart phones on biological systems has wide media coverage and public concern in recent years. The aim of this study was to explore the effects of fourth-generation cell phone radiation exposure on hematological (Total leukocyte count, Total erythrocyte count, and hemoglobin %), biochemical (Serum creatinine) parameters, and histopathological changes in the kidney and testis of Swiss albino mice. A total of 30 male Swiss albino mice weighing 45-65 g was randomly divided into three groups (n = 10). The first group A was the control group, the second group B, was exposed to 40 minutes of mobile phone radiation daily, the third group C was exposed to 60 minutes of radiation daily from two 2400 Megahertz fourth-generation connected mobile phones for 60 days, respectively. The electromagnetic radiation frequency radiometer measured the frequency of electromagnetic radiation emitted from cell phones. The specific absorption rate was calculated as 0.087 W/kg. The control group was kept under similar conditions, but the electromagnetic field was not given for the same period. All the mice were sacrificed at the end of the experiment. The blood samples were collected for hematobiochemical study, and then kidney and testis tissues were collected for histopathological study. Results of the study showed that the body weight and total erythrocyte count values were significantly (p < 0.05) decreased while total leukocyte count, hemoglobin %, and serum creatinine values were significantly (p < 0.05) increased in both the radiation exposure groups relative to the control group. Histopathological observation showed the kidney of 60 minutes exposed mice interstitial inflammation that causes marked mononuclear cellular infiltration compared to the 40 minutes and control mice. Compared to control mice, histopathological examinations of testicular tissue from the exposed mice, showed irregular in shapes and non-uniform sizes and fewer spermatogenic cells layer that leads to the larger lumen in the seminiferous tubules. It is concluded that fourth-generation cell phone radiation exposure may affect blood hemostasis and inflammation of mice's kidney and testis tissue. Based on these studies, it is important to increase public consciousness of potential adverse effects of mobile phone radiofrequency electromagnetic radiation exposure.
March-2021, 2021
Background and Aim: Nowadays, toxic chemical contaminants in food are a major food safety problem... more Background and Aim: Nowadays, toxic chemical contaminants in food are a major food safety problem in Bangladesh. Among toxic food contaminants, formalin is used to preserve fruit, vegetables, and fish, where urea is used for the whitening of rice and puffed rice. The purpose of this study was to determine the biochemical and histopathological effects on the liver of young and adult pigeons after exposure to formalin and urea contaminated feed. Materials and Methods: A total of 15 young and 15 adult pigeons were divided into control group, formaldehyde exposed group (2.5 mL formalin/kg feed), and urea exposed (1 g/kg feed) group. Each group consisted of five pigeons. After the experimentation procedures, the blood samples were collected for biochemical study, and the liver tissue was collected for histomorphological study. The statistical analysis was performed using the Student's t-test, and p<0.05 was considered as statistically significant. Results: The aspartate transamina...
Journal of Advanced Biotechnology and Experimental Therapeutics, 2020
Formaldehyde (FA), a ubiquitous environmental pollutant, has long been suspected to possess repro... more Formaldehyde (FA), a ubiquitous environmental pollutant, has long been suspected to possess reproductive toxicity. Here, we investigated the histopathological alteration of male gonads following exposure to FA-contaminated feed (40% aqueous solution of FA; 2.5 ml formalin/kg feed) in pigeons for 7 days. The mean body weights were not changed significantly in FA-contaminated feed exposed pigeons compared with control pigeons. The hemoglobin concentration was significantly decreased and serum enzyme aspartate transaminase (AST) was significantly increased in FA-exposed pigeons in comparison with control pigeons. Histologically, the structural components of the testes are the seminiferous tubules and interstitial tissues, which are surrounded by a connective tissue capsule. In control pigeons, the size and shape of seminiferous tubules were normal with a regular arrangement of spermatogenic cells. In FA-exposed pigeons, the testicular capsule was thickened and degeneration of spermatogenic cells in the seminiferous tubules was observed. The number of spermatogenic cells was significantly decreased in the seminiferous tubules of FA-exposed pigeons in comparison with control pigeons, indicating that the low exposure of FA affects the spermatogenic cells' populations in male birds. The present results suggested that FA might cause infertility in birds.
Asian Journal of Medical and Biological Research, 2019
The objective of this study was to prepare the skeletons of duck to enhance veterinary anatomy te... more The objective of this study was to prepare the skeletons of duck to enhance veterinary anatomy teaching. Five aged ducks were used in this experiment, which was collected from the Bangladesh Agricultural University poultry farm. The ducks were skinned and different segments of the body were dissected with the help of a scalpel. Then the bones were wrapped using merkin clothes, heated in 5% solution of soda water for 1 hour, and kept in that solution for 7 days for decomposing the muscles, tendon and ligaments. For proper cleaning, the bones were dipped into 10% bleaching water for 2 hours. After complete sundry, the bones were articulated using super glue for rising into a skeleton. The vertebral column was aligned with 25-gauge steel wire was inserted into the vertebral foramen. Finally, the skeletons were fixed on a wooden stand. Therefore, the skeletons were preserved and used for demonstration of osteology, and avian anatomy. This duck skeleton will be used to study avian morpho...
Bangladesh Journal of Veterinary Medicine, 2017
This study was designed to determine the shiga toxin producing genes and to investigate antibioti... more This study was designed to determine the shiga toxin producing genes and to investigate antibiotic sensitivity or resistant patterns of the <em>Escherichia coli</em> isolated from diarrheic children at Mymensingh Medical College Hospital, Bangladesh. A total of 83 stool samples were collected and screened for the detection of <em>E. coli…
British Journal of Medicine and Medical Research, 2017
Aims: This study was conducted to determine virulent genes in Escherichia coli prevalent in duck ... more Aims: This study was conducted to determine virulent genes in Escherichia coli prevalent in duck population by multiplex Polymerase Chain Reaction. Methodology: A total of 60 cloacal swab samples were collected from two duck farms of Bangladesh Agricultural University and Shamvuganj. Initially the samples were screened for the detection of E. coli on the basis of cultural, staining and biochemical properties, followed by molecular detection of E. coli using genus specific primers to amplify 16s RNA. Results: According to the results, out of 60 samples, 26 (43.33%) were confirmed to be E. coli positive. Among the E. coli positive samples, 12 (46.15%) samples were found positive for Stx-1 and 11 for Stx-2. Among 26, 11 (42.31%) samples possess both Stx-1 and Stx-2 genes, whereas Original Research Article
Asian Pacific journal of tropical biomedicine, 2014
To analyze in vivo neuro-pharmacological effects of Alpinia nigra as anxiety is a particular form... more To analyze in vivo neuro-pharmacological effects of Alpinia nigra as anxiety is a particular form of behavioral inhibition that occurs in response to novel environmental events. In present study, the extract of Alpinia nigra was evaluated for its central nervous system depressant effect using mice behavioral models, such as hole cross, open field and thiopental sodium induced sleeping time tests for its sedative properties and an elevated plus-maze test for its anxiolytic potential, respectively. In anxiolytic study, the extract displayed increased percentage of entry into open arm at the dose of 400 and 200 mg/kg. The extract produced a significant (P<0.01) increase in sleeping duration and reduction of onset of sleep compared to sodium thiopental at both doses (200 and 400 mg/kg). The extract (200 and 400 mg/kg) also showed a dose-dependent suppression of motor activity and exploratory activity of the mice in both open field and hole cross test. This study demonstrates that the...
Saudi Journal of Biological Sciences, 2021
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.