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Papers by xiaoqi jiao

Research paper thumbnail of Research Progress of Multi-signal Fluorescent Probes for Simultaneous Detection of Biothiols

Chinese Journal of Organic Chemistry, 2023

There is a close relationship between different biothiols. The changes of one biothiol level in a... more There is a close relationship between different biothiols. The changes of one biothiol level in a specific cell metabolism process may be related to the variation of another biothiol, and many diseases are the result of the combination of two or more biothiols. The simultaneous detection of divers biological thiols by fluorescence method is of particular importance for biological research and disease diagnosis. In recent years, breakthrough results have been achieved in this field. The research progress of multi-signal fluorescent probes in the field of simultaneous detection of biothiols is reviewed. According to the objects detected by the probes, the fluorescent probes are systemically classified and discussed, and the corresponding design concept, optical properties, detection mechanism, recognition performance and biological applications are summarized. At the same time, the future development and application in this field are proposed.

Research paper thumbnail of High Energy Protons on Mitoplast Membrane Interface Upon Succinate Oxidation and Its Significance in Energy Coupling Function

Acta Zoologica Sinica, 1996

The△ψ of rat liver mitoplast was measured by using Rhodamine 123 fluorescence quenching method an... more The△ψ of rat liver mitoplast was measured by using Rhodamine 123 fluorescence quenching method and the changes of the medium PH was measured with high sensitive PH electrode. There is a time difference between the △ψ of the mitoplast and the changes of medium PH induced by succinate pulse. This time difference increases with the increase of succinate concentration and decreases with the increase of the temperature. mitoplast protein concentration as well as the K+ concentration. This means that the protons ejected by redox enzyme of respiratory chain are retained at first on the interface of mitoplast membrane upon the energization. Besides, the H+/2e ratio of complex (Ⅱ +Ⅲ ) of rat liver mitoplast was obeserved to increase upon the increases of incubation temperature, mitoplast protein concentration or K+ concentration by using ferricynide pulse method. It also supports the idea mentioned above. Furthermore, the RCR was obeserved to increase upon the decrease of temperature. this set of experiment are considered to support the idea that the membrane surface protons may have some importance in the energy coupling process of mitoplast.

Research paper thumbnail of Noninvasive measurement of protein concentration

Magnetic Resonance in Medicine, Feb 1, 1996

Research paper thumbnail of Convolutional Neural Network for Universal Sentence Embeddings

This paper proposes a simple CNN model for creating general-purpose sentence embeddings that can ... more This paper proposes a simple CNN model for creating general-purpose sentence embeddings that can transfer easily across domains and can also act as effective initialization for downstream tasks. Recently, averaging the embeddings of words in a sentence has proven to be a surprisingly successful and efficient way of obtaining sentence embeddings. However, these models represent a sentence, only in terms of features of words or uni-grams in it. In contrast, our model (CSE) utilizes both features of words and n-grams to encode sentences, which is actually a generalization of these bag-of-words models. The extensive experiments demonstrate that CSE performs better than average models in transfer learning setting and exceeds the state of the art in supervised learning setting by initializing the parameters with the pre-trained sentence embeddings.

Research paper thumbnail of Improved Thermodynamic Model for Predicting RNA 3 × × × × 3 Internal Loops and NMR Structure of an Unusually Stable Loop with Three Consecutive Sheared GA Pairs

Research paper thumbnail of Parameters for an expanded nearest-neighbor model for formation of RNA duplexes with Watson-Crick pairs

Research paper thumbnail of Factors Affecting Thermodynamic Stabilities of RNA 3 × 3 Internal Loops

Research paper thumbnail of Thermodynamic Parameters for an Expanded Nearest-Neighbor Model for Formation of RNA Duplexes with Watson−Crick Base Pairs

Biochemistry, 1998

Improved thermodynamic parameters for prediction of RNA duplex formation are derived from optical... more Improved thermodynamic parameters for prediction of RNA duplex formation are derived from optical melting studies of 90 oligoribonucleotide duplexes containing only Watson-Crick base pairs. To test end or base composition effects, new sets of duplexes are included that have identical nearest neighbors, but different base compositions and therefore different ends. Duplexes with terminal GC pairs are more stable than duplexes with the same nearest neighbors but terminal AU pairs. Penalizing terminal AU base pairs by 0.45 kcal/mol relative to terminal GC base pairs significantly improves predictions of ∆G°3 7 from a nearest-neighbor model. A physical model is suggested in which the differential treatment of AU and GC ends accounts for the dependence of the total number of Watson-Crick hydrogen bonds on the base composition of a duplex. On average, the new parameters predict ∆G°3 7 , ∆H°, ∆S°, and T M within 3.2%, 6.0%, 6.8%, and 1.3°C, respectively. These predictions are within the limit of the model, based on experimental results for duplexes predicted to have identical thermodynamic parameters.

Research paper thumbnail of Noninvasive measurement of protein concentration

Magnetic Resonance in Medicine, 1996

At selected magnetic field strengths, protein and water proton spin‐lattice relaxation rates are ... more At selected magnetic field strengths, protein and water proton spin‐lattice relaxation rates are sensitive to the concentration of rotationally immobilized peptide nitrogen because of field dependent heteronuclear cross relaxation coupling between protein proton and nitrogen‐14 spins that is carried to the water by proton homonuclear cross‐relaxation. Measurement of the water proton spin‐lattice relaxation time, or a signal amplitude proportional to it, may provide a noninvasive measure of peptide bond concentration, which provides a direct measure of immobilized protein content in most tissues. The approach using protein gels in two magnetic field strengths is demonstrated. At 66.7 mT, the proton Zeeman energy matches one of the peptide nitrogen transitions dominated by the unaveraged nuclear electric quadrupole interaction; cross‐relaxation between the protons and nitrogen‐14 is efficient At 77.5 mT, the proton Zeeman energy is not matched with the nitrogen energy and proton‐nitro...

Research paper thumbnail of Research Progress of Multi-signal Fluorescent Probes for Simultaneous Detection of Biothiols

Chinese Journal of Organic Chemistry, 2023

There is a close relationship between different biothiols. The changes of one biothiol level in a... more There is a close relationship between different biothiols. The changes of one biothiol level in a specific cell metabolism process may be related to the variation of another biothiol, and many diseases are the result of the combination of two or more biothiols. The simultaneous detection of divers biological thiols by fluorescence method is of particular importance for biological research and disease diagnosis. In recent years, breakthrough results have been achieved in this field. The research progress of multi-signal fluorescent probes in the field of simultaneous detection of biothiols is reviewed. According to the objects detected by the probes, the fluorescent probes are systemically classified and discussed, and the corresponding design concept, optical properties, detection mechanism, recognition performance and biological applications are summarized. At the same time, the future development and application in this field are proposed.

Research paper thumbnail of High Energy Protons on Mitoplast Membrane Interface Upon Succinate Oxidation and Its Significance in Energy Coupling Function

Acta Zoologica Sinica, 1996

The△ψ of rat liver mitoplast was measured by using Rhodamine 123 fluorescence quenching method an... more The△ψ of rat liver mitoplast was measured by using Rhodamine 123 fluorescence quenching method and the changes of the medium PH was measured with high sensitive PH electrode. There is a time difference between the △ψ of the mitoplast and the changes of medium PH induced by succinate pulse. This time difference increases with the increase of succinate concentration and decreases with the increase of the temperature. mitoplast protein concentration as well as the K+ concentration. This means that the protons ejected by redox enzyme of respiratory chain are retained at first on the interface of mitoplast membrane upon the energization. Besides, the H+/2e ratio of complex (Ⅱ +Ⅲ ) of rat liver mitoplast was obeserved to increase upon the increases of incubation temperature, mitoplast protein concentration or K+ concentration by using ferricynide pulse method. It also supports the idea mentioned above. Furthermore, the RCR was obeserved to increase upon the decrease of temperature. this set of experiment are considered to support the idea that the membrane surface protons may have some importance in the energy coupling process of mitoplast.

Research paper thumbnail of Noninvasive measurement of protein concentration

Magnetic Resonance in Medicine, Feb 1, 1996

Research paper thumbnail of Convolutional Neural Network for Universal Sentence Embeddings

This paper proposes a simple CNN model for creating general-purpose sentence embeddings that can ... more This paper proposes a simple CNN model for creating general-purpose sentence embeddings that can transfer easily across domains and can also act as effective initialization for downstream tasks. Recently, averaging the embeddings of words in a sentence has proven to be a surprisingly successful and efficient way of obtaining sentence embeddings. However, these models represent a sentence, only in terms of features of words or uni-grams in it. In contrast, our model (CSE) utilizes both features of words and n-grams to encode sentences, which is actually a generalization of these bag-of-words models. The extensive experiments demonstrate that CSE performs better than average models in transfer learning setting and exceeds the state of the art in supervised learning setting by initializing the parameters with the pre-trained sentence embeddings.

Research paper thumbnail of Improved Thermodynamic Model for Predicting RNA 3 × × × × 3 Internal Loops and NMR Structure of an Unusually Stable Loop with Three Consecutive Sheared GA Pairs

Research paper thumbnail of Parameters for an expanded nearest-neighbor model for formation of RNA duplexes with Watson-Crick pairs

Research paper thumbnail of Factors Affecting Thermodynamic Stabilities of RNA 3 × 3 Internal Loops

Research paper thumbnail of Thermodynamic Parameters for an Expanded Nearest-Neighbor Model for Formation of RNA Duplexes with Watson−Crick Base Pairs

Biochemistry, 1998

Improved thermodynamic parameters for prediction of RNA duplex formation are derived from optical... more Improved thermodynamic parameters for prediction of RNA duplex formation are derived from optical melting studies of 90 oligoribonucleotide duplexes containing only Watson-Crick base pairs. To test end or base composition effects, new sets of duplexes are included that have identical nearest neighbors, but different base compositions and therefore different ends. Duplexes with terminal GC pairs are more stable than duplexes with the same nearest neighbors but terminal AU pairs. Penalizing terminal AU base pairs by 0.45 kcal/mol relative to terminal GC base pairs significantly improves predictions of ∆G°3 7 from a nearest-neighbor model. A physical model is suggested in which the differential treatment of AU and GC ends accounts for the dependence of the total number of Watson-Crick hydrogen bonds on the base composition of a duplex. On average, the new parameters predict ∆G°3 7 , ∆H°, ∆S°, and T M within 3.2%, 6.0%, 6.8%, and 1.3°C, respectively. These predictions are within the limit of the model, based on experimental results for duplexes predicted to have identical thermodynamic parameters.

Research paper thumbnail of Noninvasive measurement of protein concentration

Magnetic Resonance in Medicine, 1996

At selected magnetic field strengths, protein and water proton spin‐lattice relaxation rates are ... more At selected magnetic field strengths, protein and water proton spin‐lattice relaxation rates are sensitive to the concentration of rotationally immobilized peptide nitrogen because of field dependent heteronuclear cross relaxation coupling between protein proton and nitrogen‐14 spins that is carried to the water by proton homonuclear cross‐relaxation. Measurement of the water proton spin‐lattice relaxation time, or a signal amplitude proportional to it, may provide a noninvasive measure of peptide bond concentration, which provides a direct measure of immobilized protein content in most tissues. The approach using protein gels in two magnetic field strengths is demonstrated. At 66.7 mT, the proton Zeeman energy matches one of the peptide nitrogen transitions dominated by the unaveraged nuclear electric quadrupole interaction; cross‐relaxation between the protons and nitrogen‐14 is efficient At 77.5 mT, the proton Zeeman energy is not matched with the nitrogen energy and proton‐nitro...