jose valdes fernandez - Academia.edu (original) (raw)
Papers by jose valdes fernandez
Applied Microbiology and Biotechnology, 2006
cDNAs encoding two glycoside hydrolase family 1 β-glucosidases (BGL1A and BGL1B) were cloned from... more cDNAs encoding two glycoside hydrolase family 1 β-glucosidases (BGL1A and BGL1B) were cloned from the basidiomycete Phanerochaete chrysosporium, and the substrate specificities of the recombinant enzymes and the expression patterns of the two genes were investigated in relation to cellobiose metabolism by the fungus. The cDNA sequences contained open reading frames of 1,389 base pairs (bp) (bgl1A) and 1,623 bp (bgl1B),
Physical Chemistry Chemical Physics
Gas-phase spectroscopic studies of alcohol clusters offer accurate information on the influence o... more Gas-phase spectroscopic studies of alcohol clusters offer accurate information on the influence of non-covalent interactions on molecular recognition, and are of paramount importance to model supramolecular and biological chemical processes.
Thrombosis and Haemostasis, 1996
SummarycDNAs for protein C inhibitor (PCI), prepared from human liver RNA, contained two forms of... more SummarycDNAs for protein C inhibitor (PCI), prepared from human liver RNA, contained two forms of PCI, designated PCI*A and PCPB 1 . While PCI*A is identical to the published PCI sequence, PCPB differs in 4 of 1221 bp and two amino acids, A36V and K86E. Frequencies for the PCI*B allele, determined from genomic DNA, differed among ethnic groups. Frequency distribution and historical migration of modem man suggest that PCI*A arose from the PCI*B allele. Antigen levels in plasma homozygous for PCI*A or PCI*B equalled that of pooled normal plasma. K86E in PCI*B causes a charge alteration in helix D which is likely involved in heparin binding in antithrombin III but not likely involved in glycosaminoglycan binding in PCI. Kinetic studies showed that plasmas homozygous for PCI*A and PCPB are similar in their APC inhibiting properties and in their heparin sensitivity, consistent with the idea that helix D in PCI is not involved in heparin binding
Actas Del Vi Congreso De Conservacion De Bienes Culturales 1988, 1988
El Exilio Literario Asturiano De 1939 Actas Del Congreso Internacional Celebrado En La Universidad Oviedo 20 21 Y 22 De Octubre De 1999 2000 Isbn 84 922351 8 7 Pags 109 114, 2000
Angewandte Chemie (International ed. in English), Jan 10, 2014
Reported is the hydration of nanomicelles in the gas-phase using spectroscopic methods and quantu... more Reported is the hydration of nanomicelles in the gas-phase using spectroscopic methods and quantum chemical calculations. A fine-tuning of the experimental conditions allowed formation of a propofol trimer and tetramer with a water molecule and to determine the structure of the aggregates. Their electronic and IR spectra were obtained using mass-resolved laser spectroscopy, together with the number of conformational isomers for each stoichiometry. Interpretation of the spectra in the light of high-level calculations allowed determination of the cluster's structure and demonstration that the trimer of propofol with a water molecule forms cyclic hydrogen-bond networks but, on the other hand, the tetramer is big enough to encapsulate the water molecule inside its hydrophilic core. Furthermore, these hydrated nanomicelles present an unusually high binding energy, thus reflecting their high stability and their capability to trap water inside.
Transplantation Journal, 2004
The matching was performed on 1300 kidney transplants from a single center population of transpla... more The matching was performed on 1300 kidney transplants from a single center population of transplants between 1996 and 2001. When samples were taken singly from 0 to 6 mismatch, there was an increased graft survival for each match grade examined. Most commonly, differences were more pronounced with time, however, 0,1,2 mismatch groups had smaller numbers. Therefore, 0,1,2 mismatch were combined as Group I and Ͼ2 mismatch as Group II. We found approximately 2% increase in graft survival per year for Group I as compared to Group II. This 2% was additive over the first 5 years examined. Interestingly, we could show that Group I was associated with lower average trough level for both immunosuppressed groups (neoral and tacrolimus). This was empirical and not done with any prospective immunosuppressive protocol. Furthermore, the patient survival was higher in Group I (pϭ.001). Conclusion: Matching from a single large center has shown efficacy at comparing 0,1,2 mismatches to Ͼ2 mismatch. Patient survival and lower immunosuppression was indicative of shorter hospital stay, less rejection, and fewer complications.
Transplant International, 2005
Physiologia Plantarum, 1996
Phosphorus-deficient Gracilaria tenuistipitata Zhang et Xia was cultured for 15 days at two diffe... more Phosphorus-deficient Gracilaria tenuistipitata Zhang et Xia was cultured for 15 days at two different inorganic phosphate (P i ) concentrations: 3 μM (low P i treatment) or 30 μM phosphate (high P, treatment). The amount of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), phycobiliproteins, Chl a and total soluble proteins were higher in the high P i than in the low P, treatment. The total N content of the low P i plants was lower than in plants grown at high P i concentrations whereas the amount of total C was highest in low P i plants. The increase of Rubisco content in the high P i treatment (3-fold) was parallel to the enhancement of the maximum photosynthetic rate which increased 5-fold. This correspondence was also found in the low P i treatment in which Rubisco content and maximum photosynthesis did not change significantly from the initial values. The photosynthetic efficiency was also higher at high than at low P i . The high P i plants also showed higher dark respiration and growth rates. The data presented here suggest that marine macroalgae submitted to P i deficiency exhibit a decrease in growth caused not only by P i implication on energy transfer in photosynthesis and respiration, but also by the diminution of the amount of photosynthetic pigments and Rubisco.
Pathophysiology of Haemostasis and Thrombosis, 2004
Prediction of genetic risk factors for venous thrombosis might best be left for only wise soothsa... more Prediction of genetic risk factors for venous thrombosis might best be left for only wise soothsayers. Nonetheless, based on the principle that hypercoagulability, either systemic or vascular-bed-specific, predisposes to thrombosis, we venture some speculations. Hyperactivity of platelets could be caused by elevated numbers of surface glycoproteins or defective signal transduction pathways. The reported efficacy of aspirin for prevention of venous thrombosis is consistent with the prediction that certain platelet defects may increase the risk of venous thrombosis. Hyperlipidaemia is associated with hypercoagulability, and lipoproteins exhibit procoagulant (e.g. triglyceride-rich particles and oxidized low-density lipoprotein) or anticoagulant (e.g. high-density-lipoprotein’s cofactor activity for activated protein C/protein S) activities. This leads to the prediction that defects in lipids and/or lipoproteins may increase the risk for venous thrombosis. Interestingly, statins were r...
Leukemia Research, 2007
We report a case of a 64-year-old white female patient, who presented with symptomatic anemia (Hg... more We report a case of a 64-year-old white female patient, who presented with symptomatic anemia (Hgb: 6.8g/dl), thrombocytopenia (platelets: 94,000/mcl) and leukocytosis (WBC: 156,000/mcl). Peripheral blood smear revealed markedly increased white blood cell count with predominance of atypical lymphoid cells of intermediate size, moderately dense chromatin, and prominent large single nucleoli. Bone marrow aspirate smear showed predominance (78%) of atypical lymphoid cells morphologically identical to those seen in the peripheral blood. The bone marrow core biopsy was hypercellular and packed with prominent infiltrate of prolymphocytes. Immunophenotypic analysis revealed a population of monoclonal cells (75% of all -erythroid cells) characterized by CD45+, CD19+, CD20+, CD5+, HLA-DR+, CD10-, CD23+/-, CD38+ and FMC7-. The abnormal cells were restricted to kappa light chain immunoglobulin with low intensity. Cytogenetic study showed an abnormal clone of eight cells with the following karyotype: 45,X,-X,add(8)(p11.2),t(8;14)(q24;q32),add(20)ql3[8]/46,XX[12]. The relative rarity of B-PLL and the heterogeneity of clinical and laboratory parameters make it difficult to define the natural history and prognosis in all cases. The optimal treatment for B-PLL is still unknown and to date there are no reports of chromosomal abnormalities as a prognostic factor. The patient was treated with six cycles of cyclophosphamide, doxorubicin, vincristine and prednisone (CHOP). Complete remission was achieved according to the criteria defined by National Cancer Institute Working Group for CLL.
Journal of The American Society for Mass Spectrometry, 2014
Human tumor xenografts in immunodeficient mice are a very popular model to study the development ... more Human tumor xenografts in immunodeficient mice are a very popular model to study the development of cancer and to test new drug candidates. Among the parameters analyzed are the variations in the lipid composition, as they are good indicators of changes in the cellular metabolism. Here, we present a study on the distribution of lipids in xenografts of NCI-H1975 human lung cancer cells, using MALDI imaging mass spectrometry and UHPLC-ESI-QTOF. The identification of lipids directly from the tissue by MALDI was aided by the comparison with identification using ESI ionization in lipid extracts from the same xenografts. Lipids belonging to PCs, PIs, SMs, DAG, TAG, PS, PA, and PG classes were identified and their distribution over the xenograft was determined. Three areas were identified in the xenograft, corresponding to cells in different metabolic stages and to a layer of adipose tissue that covers the xenograft.
Journal of Phycology, 1997
Flores-Moya, A., Fernandez, JA and Xavier Niell, F.(1997), GROWTH PATTERN, REPRODUCTION AND SELF-... more Flores-Moya, A., Fernandez, JA and Xavier Niell, F.(1997), GROWTH PATTERN, REPRODUCTION AND SELF-THINNING IN SEAWEEDS: A RE-EVALUATION IN REPLY TO SCROSATI. Journal of Phycology, 33: 1080–1081. doi: 10.1111/j. 0022-3646.1997. 01080 ...
Journal of Neurochemistry, 1993
Protein S, an anticoagulant factor in the protein C antithrombotic pathway, was found to be synth... more Protein S, an anticoagulant factor in the protein C antithrombotic pathway, was found to be synthesized and released by six tumor cell lines of neural origin by western blotting and ELISA. The rate of synthesis ranged from three-to 1 1-fold higher than that of a microvascular endothelial cell line and 36-144% that of a hepatoma cell line. The secreted protein S displayed specific anticoagulant activity similar to that of purified plasma protein S, implying that it was fully y-carboxylated. Ten primary brain tumor tissues also expressed protein S antigen, as shown by western blot analysis. Expression of anticoagulantly active protein S by neural cells raises important questions concerning possible physiologic roles for this multidomain protein beyond its function in control of thrombosis.
The Journal of Laryngology & Otology, 1996
Giant lymph node hyperplasia (Castleman's disease) is usually reported as a solitary mediasti... more Giant lymph node hyperplasia (Castleman's disease) is usually reported as a solitary mediastinal tumour, although involvement of other anatomical sites and a multicentric form have been reported. We describe a rare case of Castleman's disease due to its localisation (the left infratemporal fossa) and histology (plasma-cell variant). A brief review of the main clinico-histological characteristics of Castleman's disease is also presented.
Applied Microbiology and Biotechnology, 2006
cDNAs encoding two glycoside hydrolase family 1 β-glucosidases (BGL1A and BGL1B) were cloned from... more cDNAs encoding two glycoside hydrolase family 1 β-glucosidases (BGL1A and BGL1B) were cloned from the basidiomycete Phanerochaete chrysosporium, and the substrate specificities of the recombinant enzymes and the expression patterns of the two genes were investigated in relation to cellobiose metabolism by the fungus. The cDNA sequences contained open reading frames of 1,389 base pairs (bp) (bgl1A) and 1,623 bp (bgl1B),
Physical Chemistry Chemical Physics
Gas-phase spectroscopic studies of alcohol clusters offer accurate information on the influence o... more Gas-phase spectroscopic studies of alcohol clusters offer accurate information on the influence of non-covalent interactions on molecular recognition, and are of paramount importance to model supramolecular and biological chemical processes.
Thrombosis and Haemostasis, 1996
SummarycDNAs for protein C inhibitor (PCI), prepared from human liver RNA, contained two forms of... more SummarycDNAs for protein C inhibitor (PCI), prepared from human liver RNA, contained two forms of PCI, designated PCI*A and PCPB 1 . While PCI*A is identical to the published PCI sequence, PCPB differs in 4 of 1221 bp and two amino acids, A36V and K86E. Frequencies for the PCI*B allele, determined from genomic DNA, differed among ethnic groups. Frequency distribution and historical migration of modem man suggest that PCI*A arose from the PCI*B allele. Antigen levels in plasma homozygous for PCI*A or PCI*B equalled that of pooled normal plasma. K86E in PCI*B causes a charge alteration in helix D which is likely involved in heparin binding in antithrombin III but not likely involved in glycosaminoglycan binding in PCI. Kinetic studies showed that plasmas homozygous for PCI*A and PCPB are similar in their APC inhibiting properties and in their heparin sensitivity, consistent with the idea that helix D in PCI is not involved in heparin binding
Actas Del Vi Congreso De Conservacion De Bienes Culturales 1988, 1988
El Exilio Literario Asturiano De 1939 Actas Del Congreso Internacional Celebrado En La Universidad Oviedo 20 21 Y 22 De Octubre De 1999 2000 Isbn 84 922351 8 7 Pags 109 114, 2000
Angewandte Chemie (International ed. in English), Jan 10, 2014
Reported is the hydration of nanomicelles in the gas-phase using spectroscopic methods and quantu... more Reported is the hydration of nanomicelles in the gas-phase using spectroscopic methods and quantum chemical calculations. A fine-tuning of the experimental conditions allowed formation of a propofol trimer and tetramer with a water molecule and to determine the structure of the aggregates. Their electronic and IR spectra were obtained using mass-resolved laser spectroscopy, together with the number of conformational isomers for each stoichiometry. Interpretation of the spectra in the light of high-level calculations allowed determination of the cluster's structure and demonstration that the trimer of propofol with a water molecule forms cyclic hydrogen-bond networks but, on the other hand, the tetramer is big enough to encapsulate the water molecule inside its hydrophilic core. Furthermore, these hydrated nanomicelles present an unusually high binding energy, thus reflecting their high stability and their capability to trap water inside.
Transplantation Journal, 2004
The matching was performed on 1300 kidney transplants from a single center population of transpla... more The matching was performed on 1300 kidney transplants from a single center population of transplants between 1996 and 2001. When samples were taken singly from 0 to 6 mismatch, there was an increased graft survival for each match grade examined. Most commonly, differences were more pronounced with time, however, 0,1,2 mismatch groups had smaller numbers. Therefore, 0,1,2 mismatch were combined as Group I and Ͼ2 mismatch as Group II. We found approximately 2% increase in graft survival per year for Group I as compared to Group II. This 2% was additive over the first 5 years examined. Interestingly, we could show that Group I was associated with lower average trough level for both immunosuppressed groups (neoral and tacrolimus). This was empirical and not done with any prospective immunosuppressive protocol. Furthermore, the patient survival was higher in Group I (pϭ.001). Conclusion: Matching from a single large center has shown efficacy at comparing 0,1,2 mismatches to Ͼ2 mismatch. Patient survival and lower immunosuppression was indicative of shorter hospital stay, less rejection, and fewer complications.
Transplant International, 2005
Physiologia Plantarum, 1996
Phosphorus-deficient Gracilaria tenuistipitata Zhang et Xia was cultured for 15 days at two diffe... more Phosphorus-deficient Gracilaria tenuistipitata Zhang et Xia was cultured for 15 days at two different inorganic phosphate (P i ) concentrations: 3 μM (low P i treatment) or 30 μM phosphate (high P, treatment). The amount of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), phycobiliproteins, Chl a and total soluble proteins were higher in the high P i than in the low P, treatment. The total N content of the low P i plants was lower than in plants grown at high P i concentrations whereas the amount of total C was highest in low P i plants. The increase of Rubisco content in the high P i treatment (3-fold) was parallel to the enhancement of the maximum photosynthetic rate which increased 5-fold. This correspondence was also found in the low P i treatment in which Rubisco content and maximum photosynthesis did not change significantly from the initial values. The photosynthetic efficiency was also higher at high than at low P i . The high P i plants also showed higher dark respiration and growth rates. The data presented here suggest that marine macroalgae submitted to P i deficiency exhibit a decrease in growth caused not only by P i implication on energy transfer in photosynthesis and respiration, but also by the diminution of the amount of photosynthetic pigments and Rubisco.
Pathophysiology of Haemostasis and Thrombosis, 2004
Prediction of genetic risk factors for venous thrombosis might best be left for only wise soothsa... more Prediction of genetic risk factors for venous thrombosis might best be left for only wise soothsayers. Nonetheless, based on the principle that hypercoagulability, either systemic or vascular-bed-specific, predisposes to thrombosis, we venture some speculations. Hyperactivity of platelets could be caused by elevated numbers of surface glycoproteins or defective signal transduction pathways. The reported efficacy of aspirin for prevention of venous thrombosis is consistent with the prediction that certain platelet defects may increase the risk of venous thrombosis. Hyperlipidaemia is associated with hypercoagulability, and lipoproteins exhibit procoagulant (e.g. triglyceride-rich particles and oxidized low-density lipoprotein) or anticoagulant (e.g. high-density-lipoprotein’s cofactor activity for activated protein C/protein S) activities. This leads to the prediction that defects in lipids and/or lipoproteins may increase the risk for venous thrombosis. Interestingly, statins were r...
Leukemia Research, 2007
We report a case of a 64-year-old white female patient, who presented with symptomatic anemia (Hg... more We report a case of a 64-year-old white female patient, who presented with symptomatic anemia (Hgb: 6.8g/dl), thrombocytopenia (platelets: 94,000/mcl) and leukocytosis (WBC: 156,000/mcl). Peripheral blood smear revealed markedly increased white blood cell count with predominance of atypical lymphoid cells of intermediate size, moderately dense chromatin, and prominent large single nucleoli. Bone marrow aspirate smear showed predominance (78%) of atypical lymphoid cells morphologically identical to those seen in the peripheral blood. The bone marrow core biopsy was hypercellular and packed with prominent infiltrate of prolymphocytes. Immunophenotypic analysis revealed a population of monoclonal cells (75% of all -erythroid cells) characterized by CD45+, CD19+, CD20+, CD5+, HLA-DR+, CD10-, CD23+/-, CD38+ and FMC7-. The abnormal cells were restricted to kappa light chain immunoglobulin with low intensity. Cytogenetic study showed an abnormal clone of eight cells with the following karyotype: 45,X,-X,add(8)(p11.2),t(8;14)(q24;q32),add(20)ql3[8]/46,XX[12]. The relative rarity of B-PLL and the heterogeneity of clinical and laboratory parameters make it difficult to define the natural history and prognosis in all cases. The optimal treatment for B-PLL is still unknown and to date there are no reports of chromosomal abnormalities as a prognostic factor. The patient was treated with six cycles of cyclophosphamide, doxorubicin, vincristine and prednisone (CHOP). Complete remission was achieved according to the criteria defined by National Cancer Institute Working Group for CLL.
Journal of The American Society for Mass Spectrometry, 2014
Human tumor xenografts in immunodeficient mice are a very popular model to study the development ... more Human tumor xenografts in immunodeficient mice are a very popular model to study the development of cancer and to test new drug candidates. Among the parameters analyzed are the variations in the lipid composition, as they are good indicators of changes in the cellular metabolism. Here, we present a study on the distribution of lipids in xenografts of NCI-H1975 human lung cancer cells, using MALDI imaging mass spectrometry and UHPLC-ESI-QTOF. The identification of lipids directly from the tissue by MALDI was aided by the comparison with identification using ESI ionization in lipid extracts from the same xenografts. Lipids belonging to PCs, PIs, SMs, DAG, TAG, PS, PA, and PG classes were identified and their distribution over the xenograft was determined. Three areas were identified in the xenograft, corresponding to cells in different metabolic stages and to a layer of adipose tissue that covers the xenograft.
Journal of Phycology, 1997
Flores-Moya, A., Fernandez, JA and Xavier Niell, F.(1997), GROWTH PATTERN, REPRODUCTION AND SELF-... more Flores-Moya, A., Fernandez, JA and Xavier Niell, F.(1997), GROWTH PATTERN, REPRODUCTION AND SELF-THINNING IN SEAWEEDS: A RE-EVALUATION IN REPLY TO SCROSATI. Journal of Phycology, 33: 1080–1081. doi: 10.1111/j. 0022-3646.1997. 01080 ...
Journal of Neurochemistry, 1993
Protein S, an anticoagulant factor in the protein C antithrombotic pathway, was found to be synth... more Protein S, an anticoagulant factor in the protein C antithrombotic pathway, was found to be synthesized and released by six tumor cell lines of neural origin by western blotting and ELISA. The rate of synthesis ranged from three-to 1 1-fold higher than that of a microvascular endothelial cell line and 36-144% that of a hepatoma cell line. The secreted protein S displayed specific anticoagulant activity similar to that of purified plasma protein S, implying that it was fully y-carboxylated. Ten primary brain tumor tissues also expressed protein S antigen, as shown by western blot analysis. Expression of anticoagulantly active protein S by neural cells raises important questions concerning possible physiologic roles for this multidomain protein beyond its function in control of thrombosis.
The Journal of Laryngology & Otology, 1996
Giant lymph node hyperplasia (Castleman's disease) is usually reported as a solitary mediasti... more Giant lymph node hyperplasia (Castleman's disease) is usually reported as a solitary mediastinal tumour, although involvement of other anatomical sites and a multicentric form have been reported. We describe a rare case of Castleman's disease due to its localisation (the left infratemporal fossa) and histology (plasma-cell variant). A brief review of the main clinico-histological characteristics of Castleman's disease is also presented.