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Papers by joydeb chanda

Indian Journal of Pharmaceutical Education and Research, 2017

Capsaicin is a pungent capsacinoid which differs in capsicum fruit within the species and the cul... more Capsaicin is a pungent capsacinoid which differs in capsicum fruit within the species and the cultivars due to its topographical diversity. Here, a comparative estimation of capsaicin in seven varieties of capsicum has been performed by RP-HPLC method. The method was carried out in reverse phase C 18 column using acetonitrile and water (1% acetic acid) as mobile phase (65:35 v/v), at the flow rate of 1ml/min. The λmax was detected at 230 nm. The calibration curves were linear in the concentration range of 1-80 µg/ml. The comparative study revealed that the capsaicin content was highest (3.12% w/w) in Capsicum annuum Cayenne whereas Capsicum cardenasii contains the lowest (0.85% w/w). The % RSD of precision and recovery was found to be < 2%, which confirms high repeatability of the method. This method can be commercialized at industrial level for ensuring the highest quality of capsicum used as raw material.

Journal of Ayurveda and Integrative Medicine, 2016

Pharmacognosy Journal, 2016

Introduction: Stereospermum suaveolens Roxb., Viscum articulatum Burm., Annona squamosa, Capsicum... more Introduction: Stereospermum suaveolens Roxb., Viscum articulatum Burm., Annona squamosa, Capsicum annuum cayenne, Ananas comosus Merrill. are used for the management of microbial infection in Ayurveda. The present study was designed to standardize the extract of S. suaveolens bark (SSB), V. articulatum aerial part (VAAP), A. squamosa leaf (ASL), C. annuum fruit (CACF), A. comosus fruit (ACF) and performed antibacterial activity. Methods: The antibacterial activity of the five extracts were evaluated against certain bacteria such as B. subtilis, B. cereus, S. aureus (gram positive); E. coli, S. typhi, and P. aureugenosa (gram negative) by disc diffusion method, time course assay, pH sensitivity assay and minimum inhibitory concentration (MICs) through broth micro-dilution method. Results: The plants extracts VAAP, ASL, and CACF showed potent inhibitory activity against S. aureus with MIC 728, 742, and 698 µg ml-1 , respectively, while CACF showed inhibitory activity against B. subtilis with MIC 690 µg ml-1. The results further demonstrated that the inhibitory activity of CACF against E. coli with MIC 760 µg ml-1. P. aeruginosa was inhibited by ASL and CACF with MIC 1100 and 1120 µg ml-1 , respectively. The ASL showed notable MBC against the tested microorganism. Moreover, all extracts were completely inactivated bacterial strains (except B. cereus, S. typhi) within 2-10 h of exposure, determined by time course assay. Conclusion: The outcomes of our study elucidate that standardized extracts of A. comosus, A. squamosa, C. annuum, S. suaveolens, and V. articulatum may be used as natural antimicrobial agents.

Journal of Ethnopharmacology, 2016

Ayurveda entails a scientific tradition of harmonious living and its origin can be traced from an... more Ayurveda entails a scientific tradition of harmonious living and its origin can be traced from ancient knowledge in Rigveda and Atharvaveda. Ayurveda is a traditional healthcare system of Indian medicine since ancient times. Several Ayurvedic medicines have been exploiting for treatment and management of various diseases in human beings. The several drugs have been developed and practiced from Ayurveda since ancient time to modern practice as &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;tradition to trend&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;. The potential of Ayurvedic medicine needs to be explored further with modern scientific validation approaches for better therapeutic leads. The present study was aimed to explore the various aspects of Ayurveda and inspired drug discovery approaches for its promotion and development. We have reviewed all the literature related to the history and application of Ayurvedic herbs. Various aspects for the quality control, standardization, chemo-profiling, and metabolite fingerprinting for quality evaluation of Ayurvedic drugs. The development of Ayurvedic drugs is gaining momentum with the perspectives of safety, efficacy and quality for promotion and management of human health. Scientific documentation, process validation and several others significant parameters are key points, which can ensure the quality, safety and effectiveness of Ayurvedic drugs. The present review highlights on the major goal of Ayurveda and their significant role in healthcare system. Ayurveda deals with several classical formulations including arka, asavas, aristas, churna, taila, vati, gutika, bhasma etc. There are several lead molecules that have been developed from the Ayurvedic herbs, which have various significant therapeutic activities. Chemo-profiling of Ayurvedic drug is essential in order to assess the quality of products. It deals with bioactive compound quantification, spurious and allied drug determination, chromatographic fingerprinting, standardization, stability and quality consistency of Ayurvedic products. Scientific validation and the documentation of Ayurvedic drugs are very essential for its quality evaluation and global acceptance. Therapeutic efficacy of Ayurvedic herbs may be enhanced with high quality, which can be achieved by identity, purity, safety, drug content, physical and biological properties. Ayurvedic medicines need be explored with the modern scientific approaches for its validation. Therefore, an attempt has been made in the present review to highlight the crucial aspects that need to be considered for the promotion and development of Ayurvedic medicine.

TANG [HUMANITAS MEDICINE], 2014

Journal of Ayurveda and Integrative Medicine, 2015

Evidence-Based Validation of Herbal Medicine, 2015

Natural Product Research, 2014

Carissa spinarum is a well-known medicinal plant which has been reported for its anthelmintic, an... more Carissa spinarum is a well-known medicinal plant which has been reported for its anthelmintic, antipyretic, antiviral, antimicrobial and antitumour activities. In this study, a reverse-phase high-performance liquid chromatographic method was developed for the simultaneous estimation of betulinic acid (BA) and ursolic acid (UA) in the methanol extract of C. spinarum root. The method was further validated for linearity, limit of detection (LOD = 3.3σ/S), limit of quantification (LOQ = 10σ/S), precision, accuracy and ruggedness. The linear response was obtained using the equation, y = 511.5x+17603 (r(2) = 0.9920) and y = 2886x+6821 (r(2) = 0.9935) for BA and UA, respectively. The LOD and LOQ were found to be 0.268 ± 0.520 μg mL(-1), 0.878 ± 0.183 μg mL(-1) for BA (0.58% w/w) and 3.140 ± 0.36 μg mL(-1), 8.820 ± 0.85 μg mL(-1) for UA (1.09% w/w), respectively. The %RSD of precision and recovery of BA and UA was &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 2.0%. The proposed method was simple, accurate, specific, precise and reproducible.

European Journal of Integrative Medicine, 2014