ines karmous - Academia.edu (original) (raw)
Papers by ines karmous
Journal of Plant Growth Regulation, Jan 24, 2022
Bulletin of Environmental Contamination and Toxicology, Jan 22, 2022
The application of Cu and CuO nanofertilizers in horticulture has been a promising strategy to pr... more The application of Cu and CuO nanofertilizers in horticulture has been a promising strategy to promote plants’ growth. In our study, increasing concentrations (10, 25, 50, 100, 250, 500, 1000, 2000 mg/L) were assessed for their inhibitory or stimulatory effects on barley ( Hordeum vulgare L.) seedlings. Our results showed an enhancement of seed germination parameters (FGP, t50, cumulative germination rate, AUC), and seedling growth parameters (roots and shoots’ lengths, fresh biomasses and dry biomasses) by the low concentrations of Cu NPs and CuO NPs, while concentrations above 500 mg/L displayed inhibiting effects. CuO NPs treatment showed a significant similitude with CuSO 4 , which confirms that CuO NPs act mainly via released Cu ions. However, Cu NPs exhibited a different behavior since the nanosized particles contribute together with Cu ions in barley response to Cu NPs. This provides an overall picture of the way these nanoparticles may behave in plant systems.
Research Square (Research Square), Jun 29, 2022
Possible impacts of nanofertilizer-mediated agriculture were assessed by studying the effects of ... more Possible impacts of nanofertilizer-mediated agriculture were assessed by studying the effects of copper and copper oxide nanoparticles (CuNP and CuO NP) on barley (Hordeum vulgare L.) seedlings. Liquid chromatography coupled to mass spectrometry and gas chromatography coupled to mass spectrometry revealed qualitative and quantitative changes in many phytochemicals. CuNP and CuO NP caused depletion of several phenolic acids and avonoids, which may reduce the antioxidant potential of foods derived from barley. An additional effect was noted as a de ciency in some bioactive compounds such as the volatile organic compounds. In addition, an imbalance in essential minerals may have impacts on biochemical and physiological functions in the human body. Alteration of nutritional quality, added to the accumulation of toxic cupric ions and hazards associated with possible induction of oxidative stress raise concerns about use of Cu and CuO based nanofertilizers in agriculture without more rigorous risk-assessment.
Environmental Science and Pollution Research, Jul 6, 2022
Plants
Carob (Ceratonia siliqua L.) is a tree crop cultivated extensively in the eastern Mediterranean r... more Carob (Ceratonia siliqua L.) is a tree crop cultivated extensively in the eastern Mediterranean regions but that has become naturalized in other regions as well. The present study focused on the green synthesis of zinc oxide nanoparticles (ZnONPs) from Carob and their evaluation for antimicrobial activity in bacteria and fungi. The synthesized ZnONPs showed strong antibacterial activity against Staphylococcus aureus ATCC 25 923 (92%). The NPs inhibited the growth of pathogenic yeast strains, including Candida albicans ATCC90028, Candida krusei ATCC6258, and Candida neoformans ATCC14116, by 90%, 91%, and 82%, respectively, compared to the control. Fungal inhibition zones with the ZnONPs were 88.67% and 90%, respectively, larger for Aspergillus flavus 15UA005 and Aspergillus fumigatus ATCC204305, compared to control fungal growth. This study provides novel information relevant for plant-based development of new and potentially antimicrobial ZnONPs based on extracts. In particular, the...
Chemistry & Biodiversity, 2021
The process of soil salinization and the preponderance of saline water sources all over the world... more The process of soil salinization and the preponderance of saline water sources all over the world represent one of the most harmful abiotic stress to plant growth. This pointed to the importance of obtaining plants which are tolerant or resistant to salt, considering that projection of climate change for the coming years indicate an increase in temperature and rain scarcity. In the current study, the effect of NaCl was investigated on germinating seeds of Lathyrus sativus L., Vicia sativa L., Vigna radiata L. R.Wilczek and Vigna unguiculata L. Walp., by combining physiological, biochemical, biostatistical and ultrastructural analyses. Our results revealed that germination was not influenced by high NaCl concentrations, while seedling growth was affected even at low NaCl concentrations, probably due to an alteration in water uptake and in organic matter biosynthesis. Nevertheless, the synthesis of antioxidant enzymes, phenolic acids and flavonoids was registered in all species, which...
Environmental Geochemistry and Health, 2021
The present article aims to assess the phytotoxic effects of copper and zinc oxide nanoparticles ... more The present article aims to assess the phytotoxic effects of copper and zinc oxide nanoparticles (Cu NPs, ZnO NPs) on mung bean (Vigna radiata L.) and their possible risk on food quality and safety. We also study the molecular mechanisms underlying the toxicity of nanosized Cu and ZnO. Seeds of mung bean were germinated under increasing concentrations of Cu NPs and ZnO NPs (10, 100, 1000, 2000 mg/L). We analyzed levels of free amino acids, total soluble sugars, minerals, polyphenols and antioxidant capacity. Our results showed that depending on the concentrations used of Cu NPs and ZnO NPs, the physiology of seed germination and embryo growth were modified. Both free metal ions and nanoparticles themselves may impact plant cellular and physiological processes. At 10 mg/L, an improvement of the nutritive properties, in terms of content in free amino acids, total soluble sugars, essential minerals, antioxidant polyphenols and flavonoids, was shown. However, higher concentrations (100–2000 mg/L) caused an alteration in the nutritional balance, which was revealed by the decrease in contents and quality of phenolic compounds, macronutrients (Na, Mg, Ca) and micronutrients (Cu, Fe, Mn, Zn, K). The overall effects of Cu and ZnO nanoparticles seem to interfere with the bioavailability of mineral and organic nutrients and alter the beneficial properties of the antioxidant phytochemicals, mineral compounds, phenolic acids and flavonoids. This may result in a potential hazard to human food and health, at some critical doses of nanofertilizers. This study may contribute in the guidelines to the safe use of nanofertilizers or nanosafety, for more health benefit and less potential risks.
Biological Trace Element Research, 2019
The aim of this work is to review the current knowledge on the efficiency of plant-based synthesi... more The aim of this work is to review the current knowledge on the efficiency of plant-based synthesized nanoparticles in medical field, particularly in the prevention, diagnosis, and therapy of cancer. For this, we examine the advantages of nanotechnological tools. Besides, a particular attention was given to understand the mechanism by which plant-based bioengineered nanoparticles can interact with components of cancerous cells. Green biosynthesized nanoparticles seem to be novel tool for prognostic biomarkers for cancer diagnosis and drug delivery in tumor cells. They can act either by leading to the damage of tumor cells, or by the protection of healthy cells, via mechanisms involving the specific properties of nanoparticles themselves and the antioxidative and antitumor properties found in plants. However, special attention should be given to the choice of plant species, extracts, and the toxic dose of some phytocompounds during the biosynthesis process. An increase in metal or trace element release from metal and metal oxide biosynthesized nanoparticles can lead to greater oxidative stress, which is associated with higher risk of cancer. Hence, plant-based nanosystems should be more developed to increase their specific targeting of the cancerous cells, in order to preserve the healthy ones.
Journal of Plant Interactions, Dec 4, 2017
The ameliorating effects of four exogenous effectors were investigated in germinating pea seeds e... more The ameliorating effects of four exogenous effectors were investigated in germinating pea seeds exposed to copper excess. The results showed that the application of IAA, GA 3 , Ca or citric acid alleviated Cu-induced inhibition of growth and simultaneously reduced the oxidative stress injury, particularly contents of hydrogen peroxide, malondialdehyde and carbonyl groups. The improving effects can probably be mediated by the decreases in lipoperoxidation and protein oxidation as evidenced by changes in antioxidant enzyme activities. In addition, the efficiency of this recovery was compared within two types of treatments. Obtained results demonstrated that the stress abruption by the addition of effectors after three days of Cu application (treatment of type II) seems to be more effective than the simultaneous application of 'Cu + effectors' at the beginning of germination (treatment of type I). Data could provide some clues to physiological and biochemical mechanisms of the response of germinating seeds to the addition of chemicals under heavy metal stress.
Journal of Plant Physiology, Jul 1, 2018
This work aims to give more insight into mechanisms of action of cadmium (Cd) on germinating pea ... more This work aims to give more insight into mechanisms of action of cadmium (Cd) on germinating pea seeds (Pisum sativum L. var. douce province), specifically the different ways by which Cd cations may interfere with the principal factors involved during germination process, notably storage proteins mobilization, amino acids freeing and proteolytic activities. Obtained results revealed that the process of hydrolysis of main storage proteins showed a significant disruption, which resulted in the decrease of the release of free amino acids, thus imposing a lack in nitrogen supply of essential nutrients to growing embryo under Cd stress. This hypothesis was evidenced by Cd-induced changes occurring in main purified protein fractions; Albumins, Legumins and Vicilins, during their breakdown. Besides, at enzymatic level, the activities of main proteases responsible for this hydrolysis were altered. Indeed, assays using synthetic substrates and specific protease inhibitors followed by protease activity measurements demonstrated that Cd inhibited drastically the total azocaseinolytic activity (ACA) and activities of different proteolytic classes: cysteine-, aspartic-, serine-and metallo-endopeptidases (EP), leucine-and proline-aminopeptidases (LAP and PAP, respectively), and glycine-carboxypeptidases (Gly-CP). The data here presented may suggest that the vulnerability of the embryonic axes towards Cd toxicity could be explained as a result of eventual disruption of metabolic pathways that affect mobilization of reserves and availability of nutrients. In vitro studies suggest that Cd cations may act either directly on the catalytic sites of the proteolytic enzymes, which may cause their deactivation, or indirectly via the generation of oxidative stress and overproduction of free radicals that can interact with enzymes, by altering their activity and structure.
Research & Reviews: Journal of Biology, 2016
The present study showed the evidence of the generation of an oxidative stress in the growing emb... more The present study showed the evidence of the generation of an oxidative stress in the growing embryos of germinating pea (Pisum sativum L. cv. douce province) seeds, after exposure to 200 μM cadmium chloride (CdCl2). Hence, we focused on understanding Cd induced oxidative stress, its consequences and the biological significance of some enzymatic and non-enzymatic antioxidants as potential selection criteria for improving tolerance to oxidative stress have been discussed. The effects of Cd on some indicators of oxidative stress were analyzed in order to study relationship between metal toxicity, oxidative stress and detoxification responses. Obtained results revealed a protection of embryonic axes growth and metabolism against the heavy metal up to day 6 of germination, beyond then severe metabolic disturbances have been observed leading to oxidative injury to proteins and marked disruption of the structural integrity of membranes and cellular homeostasis. This might be associated to...
The present work aims to provide insight on the role of phytohormone application in developing ef... more The present work aims to provide insight on the role of phytohormone application in developing efficient practical defense strategies to improve plants tolerance under heavy metal contamination. For this purpose, pea (Pisum sativum L.) seeds were germinated in an aqueous solution of 200 μM CuCl2 up to the 3 rd day and then continued to germinate in the presence of distilled water (stress cessation) or were subjected to following combinations: Cu + 1 μM IAA and Cu + 1 μM GA3 for 3 additional days. The results showed that copper excess induced oxidative stress in germinating seeds, which resulted in changes of the redox state of glutathione and cysteine, and proteomics revealed Cu-induced modifications of thiols (SH) and carbonyls (CO) (indicators of protein oxidation). However, application of IAA or GA3 in the germination medium after 3 days of Cu exposure alleviated toxicity on seedlings, despite the persistence of Cu up to 6 day. This improving effect seems to be mediated by a cell...
Journal of plant physiology, Jan 9, 2017
The present work aims to provide insight on the role of phytohormone application in developing ef... more The present work aims to provide insight on the role of phytohormone application in developing efficient practical defense strategies to improve plants tolerance under heavy metal contamination. For this purpose, pea (Pisum sativum L.) seeds were germinated in an aqueous solution of 200μM CuCl2 up to the 3rd day and then continued to germinate in the presence of distilled water (stress cessation) or were subjected to following combinations: Cu+1μM IAA and Cu+1μM GA3 for 3 additional days. The results showed that copper excess induced oxidative stress in germinating seeds, which resulted in changes of the redox state of glutathione and cysteine, and proteomics revealed Cu-induced modifications of thiols (SH) and carbonyls (CO) (indicators of protein oxidation). However, application of IAA or GA3 in the germination medium after 3days of Cu exposure alleviated toxicity on seedlings, despite the persistence of Cu up to 6th day. This improving effect seems to be mediated by a cell Cu acc...
Pesticide Biochemistry and Physiology
Plant Disease
Nanoscale materials are promising tools for managing plant diseases and are becoming important co... more Nanoscale materials are promising tools for managing plant diseases and are becoming important components in the current agritech revolution. However, adopting modern methodologies requires a broad understanding of their effectiveness in solving target problems and their effects on the environment and food chain. Furthermore, it is paramount that such technologies are mechanistically and economically feasible for growers to adopt in order to be sustainable. This Feature Article summarizes the latest findings on the role of nanoscale materials in managing agricultural plant pathogens. Herein, we discussed the benefits and limitations of using nanoscale materials in plant disease management and their potential impacts on the environment and global food security.
Polish Journal of Environmental Studies
Zinc based nanofertilizers may be useful tools in improving crop culture, especially in Zinc defi... more Zinc based nanofertilizers may be useful tools in improving crop culture, especially in Zinc deficient soil. The present study aims to investigate the role of nanosized zinc oxide particles (ZnO NPs, diameter<100 nm) in modulating seed germination, embryo nutrition and growth of grass pea (Lathyrus sativus L.). Our data revealed ameliorating or inhibiting effects depending of the concentration of ZnO NPs administrated. At metabolic level, the growing embryonic axes seem to cope with induced oxidative stress, by enhancing hydrogen peroxide scavenging capacity. We revealed interesting regulatory mechanisms evolved within the embryonic cells to limit the oxidative damages induced by ZnO NPs and Zinc sulfate when applied at low concentrations (0.01 mg mL-1, 0.1 mg mL-1). Nonetheless, at high concentrations (1 mg mL-1, 10 mg mL-1), ZnO NPs led to drastic perturbations in the metabolism, which resulted in the inhibition of root and seedling growth. Our work may bring novel insight into...
Journal of Plant Growth Regulation, Jan 24, 2022
Bulletin of Environmental Contamination and Toxicology, Jan 22, 2022
The application of Cu and CuO nanofertilizers in horticulture has been a promising strategy to pr... more The application of Cu and CuO nanofertilizers in horticulture has been a promising strategy to promote plants’ growth. In our study, increasing concentrations (10, 25, 50, 100, 250, 500, 1000, 2000 mg/L) were assessed for their inhibitory or stimulatory effects on barley ( Hordeum vulgare L.) seedlings. Our results showed an enhancement of seed germination parameters (FGP, t50, cumulative germination rate, AUC), and seedling growth parameters (roots and shoots’ lengths, fresh biomasses and dry biomasses) by the low concentrations of Cu NPs and CuO NPs, while concentrations above 500 mg/L displayed inhibiting effects. CuO NPs treatment showed a significant similitude with CuSO 4 , which confirms that CuO NPs act mainly via released Cu ions. However, Cu NPs exhibited a different behavior since the nanosized particles contribute together with Cu ions in barley response to Cu NPs. This provides an overall picture of the way these nanoparticles may behave in plant systems.
Research Square (Research Square), Jun 29, 2022
Possible impacts of nanofertilizer-mediated agriculture were assessed by studying the effects of ... more Possible impacts of nanofertilizer-mediated agriculture were assessed by studying the effects of copper and copper oxide nanoparticles (CuNP and CuO NP) on barley (Hordeum vulgare L.) seedlings. Liquid chromatography coupled to mass spectrometry and gas chromatography coupled to mass spectrometry revealed qualitative and quantitative changes in many phytochemicals. CuNP and CuO NP caused depletion of several phenolic acids and avonoids, which may reduce the antioxidant potential of foods derived from barley. An additional effect was noted as a de ciency in some bioactive compounds such as the volatile organic compounds. In addition, an imbalance in essential minerals may have impacts on biochemical and physiological functions in the human body. Alteration of nutritional quality, added to the accumulation of toxic cupric ions and hazards associated with possible induction of oxidative stress raise concerns about use of Cu and CuO based nanofertilizers in agriculture without more rigorous risk-assessment.
Environmental Science and Pollution Research, Jul 6, 2022
Plants
Carob (Ceratonia siliqua L.) is a tree crop cultivated extensively in the eastern Mediterranean r... more Carob (Ceratonia siliqua L.) is a tree crop cultivated extensively in the eastern Mediterranean regions but that has become naturalized in other regions as well. The present study focused on the green synthesis of zinc oxide nanoparticles (ZnONPs) from Carob and their evaluation for antimicrobial activity in bacteria and fungi. The synthesized ZnONPs showed strong antibacterial activity against Staphylococcus aureus ATCC 25 923 (92%). The NPs inhibited the growth of pathogenic yeast strains, including Candida albicans ATCC90028, Candida krusei ATCC6258, and Candida neoformans ATCC14116, by 90%, 91%, and 82%, respectively, compared to the control. Fungal inhibition zones with the ZnONPs were 88.67% and 90%, respectively, larger for Aspergillus flavus 15UA005 and Aspergillus fumigatus ATCC204305, compared to control fungal growth. This study provides novel information relevant for plant-based development of new and potentially antimicrobial ZnONPs based on extracts. In particular, the...
Chemistry & Biodiversity, 2021
The process of soil salinization and the preponderance of saline water sources all over the world... more The process of soil salinization and the preponderance of saline water sources all over the world represent one of the most harmful abiotic stress to plant growth. This pointed to the importance of obtaining plants which are tolerant or resistant to salt, considering that projection of climate change for the coming years indicate an increase in temperature and rain scarcity. In the current study, the effect of NaCl was investigated on germinating seeds of Lathyrus sativus L., Vicia sativa L., Vigna radiata L. R.Wilczek and Vigna unguiculata L. Walp., by combining physiological, biochemical, biostatistical and ultrastructural analyses. Our results revealed that germination was not influenced by high NaCl concentrations, while seedling growth was affected even at low NaCl concentrations, probably due to an alteration in water uptake and in organic matter biosynthesis. Nevertheless, the synthesis of antioxidant enzymes, phenolic acids and flavonoids was registered in all species, which...
Environmental Geochemistry and Health, 2021
The present article aims to assess the phytotoxic effects of copper and zinc oxide nanoparticles ... more The present article aims to assess the phytotoxic effects of copper and zinc oxide nanoparticles (Cu NPs, ZnO NPs) on mung bean (Vigna radiata L.) and their possible risk on food quality and safety. We also study the molecular mechanisms underlying the toxicity of nanosized Cu and ZnO. Seeds of mung bean were germinated under increasing concentrations of Cu NPs and ZnO NPs (10, 100, 1000, 2000 mg/L). We analyzed levels of free amino acids, total soluble sugars, minerals, polyphenols and antioxidant capacity. Our results showed that depending on the concentrations used of Cu NPs and ZnO NPs, the physiology of seed germination and embryo growth were modified. Both free metal ions and nanoparticles themselves may impact plant cellular and physiological processes. At 10 mg/L, an improvement of the nutritive properties, in terms of content in free amino acids, total soluble sugars, essential minerals, antioxidant polyphenols and flavonoids, was shown. However, higher concentrations (100–2000 mg/L) caused an alteration in the nutritional balance, which was revealed by the decrease in contents and quality of phenolic compounds, macronutrients (Na, Mg, Ca) and micronutrients (Cu, Fe, Mn, Zn, K). The overall effects of Cu and ZnO nanoparticles seem to interfere with the bioavailability of mineral and organic nutrients and alter the beneficial properties of the antioxidant phytochemicals, mineral compounds, phenolic acids and flavonoids. This may result in a potential hazard to human food and health, at some critical doses of nanofertilizers. This study may contribute in the guidelines to the safe use of nanofertilizers or nanosafety, for more health benefit and less potential risks.
Biological Trace Element Research, 2019
The aim of this work is to review the current knowledge on the efficiency of plant-based synthesi... more The aim of this work is to review the current knowledge on the efficiency of plant-based synthesized nanoparticles in medical field, particularly in the prevention, diagnosis, and therapy of cancer. For this, we examine the advantages of nanotechnological tools. Besides, a particular attention was given to understand the mechanism by which plant-based bioengineered nanoparticles can interact with components of cancerous cells. Green biosynthesized nanoparticles seem to be novel tool for prognostic biomarkers for cancer diagnosis and drug delivery in tumor cells. They can act either by leading to the damage of tumor cells, or by the protection of healthy cells, via mechanisms involving the specific properties of nanoparticles themselves and the antioxidative and antitumor properties found in plants. However, special attention should be given to the choice of plant species, extracts, and the toxic dose of some phytocompounds during the biosynthesis process. An increase in metal or trace element release from metal and metal oxide biosynthesized nanoparticles can lead to greater oxidative stress, which is associated with higher risk of cancer. Hence, plant-based nanosystems should be more developed to increase their specific targeting of the cancerous cells, in order to preserve the healthy ones.
Journal of Plant Interactions, Dec 4, 2017
The ameliorating effects of four exogenous effectors were investigated in germinating pea seeds e... more The ameliorating effects of four exogenous effectors were investigated in germinating pea seeds exposed to copper excess. The results showed that the application of IAA, GA 3 , Ca or citric acid alleviated Cu-induced inhibition of growth and simultaneously reduced the oxidative stress injury, particularly contents of hydrogen peroxide, malondialdehyde and carbonyl groups. The improving effects can probably be mediated by the decreases in lipoperoxidation and protein oxidation as evidenced by changes in antioxidant enzyme activities. In addition, the efficiency of this recovery was compared within two types of treatments. Obtained results demonstrated that the stress abruption by the addition of effectors after three days of Cu application (treatment of type II) seems to be more effective than the simultaneous application of 'Cu + effectors' at the beginning of germination (treatment of type I). Data could provide some clues to physiological and biochemical mechanisms of the response of germinating seeds to the addition of chemicals under heavy metal stress.
Journal of Plant Physiology, Jul 1, 2018
This work aims to give more insight into mechanisms of action of cadmium (Cd) on germinating pea ... more This work aims to give more insight into mechanisms of action of cadmium (Cd) on germinating pea seeds (Pisum sativum L. var. douce province), specifically the different ways by which Cd cations may interfere with the principal factors involved during germination process, notably storage proteins mobilization, amino acids freeing and proteolytic activities. Obtained results revealed that the process of hydrolysis of main storage proteins showed a significant disruption, which resulted in the decrease of the release of free amino acids, thus imposing a lack in nitrogen supply of essential nutrients to growing embryo under Cd stress. This hypothesis was evidenced by Cd-induced changes occurring in main purified protein fractions; Albumins, Legumins and Vicilins, during their breakdown. Besides, at enzymatic level, the activities of main proteases responsible for this hydrolysis were altered. Indeed, assays using synthetic substrates and specific protease inhibitors followed by protease activity measurements demonstrated that Cd inhibited drastically the total azocaseinolytic activity (ACA) and activities of different proteolytic classes: cysteine-, aspartic-, serine-and metallo-endopeptidases (EP), leucine-and proline-aminopeptidases (LAP and PAP, respectively), and glycine-carboxypeptidases (Gly-CP). The data here presented may suggest that the vulnerability of the embryonic axes towards Cd toxicity could be explained as a result of eventual disruption of metabolic pathways that affect mobilization of reserves and availability of nutrients. In vitro studies suggest that Cd cations may act either directly on the catalytic sites of the proteolytic enzymes, which may cause their deactivation, or indirectly via the generation of oxidative stress and overproduction of free radicals that can interact with enzymes, by altering their activity and structure.
Research & Reviews: Journal of Biology, 2016
The present study showed the evidence of the generation of an oxidative stress in the growing emb... more The present study showed the evidence of the generation of an oxidative stress in the growing embryos of germinating pea (Pisum sativum L. cv. douce province) seeds, after exposure to 200 μM cadmium chloride (CdCl2). Hence, we focused on understanding Cd induced oxidative stress, its consequences and the biological significance of some enzymatic and non-enzymatic antioxidants as potential selection criteria for improving tolerance to oxidative stress have been discussed. The effects of Cd on some indicators of oxidative stress were analyzed in order to study relationship between metal toxicity, oxidative stress and detoxification responses. Obtained results revealed a protection of embryonic axes growth and metabolism against the heavy metal up to day 6 of germination, beyond then severe metabolic disturbances have been observed leading to oxidative injury to proteins and marked disruption of the structural integrity of membranes and cellular homeostasis. This might be associated to...
The present work aims to provide insight on the role of phytohormone application in developing ef... more The present work aims to provide insight on the role of phytohormone application in developing efficient practical defense strategies to improve plants tolerance under heavy metal contamination. For this purpose, pea (Pisum sativum L.) seeds were germinated in an aqueous solution of 200 μM CuCl2 up to the 3 rd day and then continued to germinate in the presence of distilled water (stress cessation) or were subjected to following combinations: Cu + 1 μM IAA and Cu + 1 μM GA3 for 3 additional days. The results showed that copper excess induced oxidative stress in germinating seeds, which resulted in changes of the redox state of glutathione and cysteine, and proteomics revealed Cu-induced modifications of thiols (SH) and carbonyls (CO) (indicators of protein oxidation). However, application of IAA or GA3 in the germination medium after 3 days of Cu exposure alleviated toxicity on seedlings, despite the persistence of Cu up to 6 day. This improving effect seems to be mediated by a cell...
Journal of plant physiology, Jan 9, 2017
The present work aims to provide insight on the role of phytohormone application in developing ef... more The present work aims to provide insight on the role of phytohormone application in developing efficient practical defense strategies to improve plants tolerance under heavy metal contamination. For this purpose, pea (Pisum sativum L.) seeds were germinated in an aqueous solution of 200μM CuCl2 up to the 3rd day and then continued to germinate in the presence of distilled water (stress cessation) or were subjected to following combinations: Cu+1μM IAA and Cu+1μM GA3 for 3 additional days. The results showed that copper excess induced oxidative stress in germinating seeds, which resulted in changes of the redox state of glutathione and cysteine, and proteomics revealed Cu-induced modifications of thiols (SH) and carbonyls (CO) (indicators of protein oxidation). However, application of IAA or GA3 in the germination medium after 3days of Cu exposure alleviated toxicity on seedlings, despite the persistence of Cu up to 6th day. This improving effect seems to be mediated by a cell Cu acc...
Pesticide Biochemistry and Physiology
Plant Disease
Nanoscale materials are promising tools for managing plant diseases and are becoming important co... more Nanoscale materials are promising tools for managing plant diseases and are becoming important components in the current agritech revolution. However, adopting modern methodologies requires a broad understanding of their effectiveness in solving target problems and their effects on the environment and food chain. Furthermore, it is paramount that such technologies are mechanistically and economically feasible for growers to adopt in order to be sustainable. This Feature Article summarizes the latest findings on the role of nanoscale materials in managing agricultural plant pathogens. Herein, we discussed the benefits and limitations of using nanoscale materials in plant disease management and their potential impacts on the environment and global food security.
Polish Journal of Environmental Studies
Zinc based nanofertilizers may be useful tools in improving crop culture, especially in Zinc defi... more Zinc based nanofertilizers may be useful tools in improving crop culture, especially in Zinc deficient soil. The present study aims to investigate the role of nanosized zinc oxide particles (ZnO NPs, diameter<100 nm) in modulating seed germination, embryo nutrition and growth of grass pea (Lathyrus sativus L.). Our data revealed ameliorating or inhibiting effects depending of the concentration of ZnO NPs administrated. At metabolic level, the growing embryonic axes seem to cope with induced oxidative stress, by enhancing hydrogen peroxide scavenging capacity. We revealed interesting regulatory mechanisms evolved within the embryonic cells to limit the oxidative damages induced by ZnO NPs and Zinc sulfate when applied at low concentrations (0.01 mg mL-1, 0.1 mg mL-1). Nonetheless, at high concentrations (1 mg mL-1, 10 mg mL-1), ZnO NPs led to drastic perturbations in the metabolism, which resulted in the inhibition of root and seedling growth. Our work may bring novel insight into...