mandana jafari - Academia.edu (original) (raw)
Papers by mandana jafari
Current Clinical Pharmacology, 2019
Background: Serotonin 5-HT3 receptor antagonists such as ondansetron have been investigated to at... more Background: Serotonin 5-HT3 receptor antagonists such as ondansetron have been investigated to attenuate opioid withdrawal signs in studies. Objective: Therefore, we designed a randomized double-blinded placebo-controlled trial to evaluate this effect on opioid-addicted patients who were admitted to the orthopedic department for surgery due to bone fractures. Methods: Male adults who were addicted to opioids, aged 18 to 79 years were enrolled (n=96) and randomized into intravenous doses (4 & 8 mg) of ondansetron (n=32) and placebo (n=32). The vital signs, withdrawal symptoms and the frequency requirement of fentanyl were recorded during anesthesia, and opioid (pethidine) analgesic was received during the recovery period. Outcome parameters were analyzed for reduction of withdrawal symptoms in addicted adults. Results: We indicated that ondansetron demonstrated significant differences with few vital outcomes including systolic blood pressure (SBP) 20 (SBP3) and 50 min (SBP4) after in...
Materials Research Bulletin, 2018
Cytotoxic and antioxidant activity of the biogenic bismuth nanoparticles produced by Delftia sp.
Biocatalysis and Agricultural Biotechnology, 2018
, Cytotoxic and antibacterial activities of biologically synthesized gold nanoparticles assisted ... more , Cytotoxic and antibacterial activities of biologically synthesized gold nanoparticles assisted by Micrococcus yunnanensis strain J2, Biocatalysis and Agricultural Biotechnology,
IET Nanobiotechnology, 2018
In this study, we evaluated the cytotoxicity underlying mechanisms of biogenic tellurium (Te) nan... more In this study, we evaluated the cytotoxicity underlying mechanisms of biogenic tellurium (Te) nanorods (NRs) produced by the Pseudomonas pseudoalcaligenes strain Te on the PC12 cell line. The half-maximal inhibitory concentration (IC50) value was estimated at 5.05±0.07 ng/ml for biogenic Te NRs and 2.44±0.38 ng/ml for K2TeO3, respectively. The viability of PC12 was inhibited concentration-dependent at doses of 1, 2.5, 5, 10, and 20 ng/ml. Te NRs principally induced late apoptosis or necrosis at IC50 concentration, without effect on caspase-3 activities. Furthermore, Te NRs reduced glutathione (GSH) and enhanced malondialdehyde (MDA) levels, and also reduced superoxide dismutase (SOD) and catalase (CAT) activities. These findings revealed that biogenic Te NRs were less toxic than K2TeO3. Additionally, they induced cytotoxity toward the PC12 cell line through the activation of late apoptosis independent of the caspase pathway, and may also enhance oxidative stress (OS) in the nervous system.
IET nanobiotechnology, 2018
This study was purposed to examine the cytotoxicity and functions of biologically synthesised bis... more This study was purposed to examine the cytotoxicity and functions of biologically synthesised bismuth nanoparticles (Bi NPs) produced by sp. SFG on human colon adenocarcinoma cell line of HT-29. The structural properties of Bi NPs were investigated using transmission electron microscopy, energy dispersive X-ray, and X-ray diffraction techniques. The cytotoxic effects of Bi NPs were analysed using flow cytometry cell apoptosis while western blot analyses were applied to analyse the cleaved caspase-3 expression. Oxidative stress (OS) damage was determined using the measurement of the glutathione (GSH) and malondialdehyde (MDA) levels and antioxidant activity of superoxide dismutase (SOD) and catalase (CAT) levels. The half maximal inhibitory concentration (IC) value of Bi NPs was measured to be 28.7 ± 1.4 µg/ml on HT-29 cell line. The viability of HT-29 represented a concentration-dependent pattern (5-80 µg/ml). The mode of Bi NPs induced apoptosis was found to be mainly related to la...
European journal of medicinal chemistry, Jan 25, 2018
A novel series of coumarin-lipoic acid conjugates were synthesized via cycloaddition click reacti... more A novel series of coumarin-lipoic acid conjugates were synthesized via cycloaddition click reaction to find out new multi-target-directed ligands (MTDLs) for treatment of Alzheimer's disease (AD). All of synthesized compounds were screened for neuroprotective and anti-cholinesterase activities. Based on primary screening, two compounds (5 and 11) were subjected to further biological evaluations. In particular, compound 11 which was the most potent AChE inhibitor showed good inhibitory effect on Aβ-aggregation and intracellular ROS (reactive oxygen species) formation, as well as the ability of selective bio-metal chelation and neuroprotection against HO- and Aβ-induced cytotoxicity. In the light of these results, the applied hybridization approach introduced new promising lead compound with desired multifunctional properties, being useful in the treatment of Alzheimer's disease.
![Research paper thumbnail of New racemic annulated pyrazolo[1,2-b]phthalazines as tacrine-like AChE inhibitors with potential use in Alzheimer's disease](https://attachments.academia-assets.com/102425609/thumbnails/1.jpg)
](European Journal of Medicinal Chemistry, 2017
A series of tacrine-like compounds 7a-u were designed and synthesized as potent AChE inhibitors w... more A series of tacrine-like compounds 7a-u were designed and synthesized as potent AChE inhibitors with high selectivity, low toxicity on hepatocytes, neuroprotective and and βamyloid aggregation inhibitory activity.
Journal of Trace Elements in Medicine and Biology, 2017
A lactobacillus strain (L. brevis LSe tolerates 401.3 mg L-1 of SeO3 2-) was isolated. The pe... more A lactobacillus strain (L. brevis LSe tolerates 401.3 mg L-1 of SeO3 2-) was isolated. The performed probiotic tests revealed acceptable probiotic characteristics. DPPH assay represented moderate antioxidant activity of the Se-enriched L. brevis.
Journal of Trace Elements in Medicine and Biology, 2017
The present study was designed for microwave assisted synthesis of zinc nanoparticles (Zn NPs) us... more The present study was designed for microwave assisted synthesis of zinc nanoparticles (Zn NPs) using Lavandula vera leaf extract in the presence of ZnSO 4 (1 mM). The biogenic Zn NPs were then characterized using scanning electron microscopy (SEM), energy dispersive X-ray (EDX), X-ray diffraction spectroscopy (XRD), UV-visible spectroscopy, and Fourier transform infrared spectroscopy (FTIR) techniques. Thereafter, the cytotoxic effect of ZnSO 4 and Zn NPs on different cell lines was investigated by MTT-based cytotoxicity assay and their antioxidant properties were assessed using DPPH scavenging activity and reducing power assay. The SEM micrograph showed that the Zn NPs had spherical shape with the size range of 30-80 nm. For A549, MCF-7, HT-29, and Caco-2 cell lines treated with Zn NPs, the concentration necessary causing 50% cell death (IC 50) was found to be 22.3 ± 1.1 g mL −1 , 86 ± 3.7 g mL −1 , 10.9 ± 0.5 g mL −1 , and 56.2 ± 2.8 g mL −1 , respectively. In the case of ZnSO 4 , the same results (IC 50) were observed at concentration of 81.6 ± 1.3 g mL −1 (A549), 121.0 ± 2.4 g mL −1 (MCF-7), 43.0 ± 1.4 g mL −1 (HT-29), and 85.7 ± 2.3 g mL −1 (Caco-2). The obtained results of antioxidant activity showed that the IC 50 values of butylated hydroxyanisole (BHA) and Zn NPs were 44 g mL −1 and 65.3 g mL −1 , respectively, while ZnSO 4 at concentration of 200 g mL −1 exhibited only 10.9% DPPH radical scavenging effect. Moreover, the reducing power of Zn NPs and BHA were significantly higher than ZnSO 4 (p < 0.05). To sum up, application of L. vera leaf extract combined with microwave heating energy led to simple and fast formation of Zn nanostructures exhibited higher antioxidant and cytotoxic activity compared to soluble Zn +2 ions. However, identification of the related mechanisms merit further studies.
Materials Science and Engineering: C, 2015
European Journal of Medicinal Chemistry, 2014
A series of N-benzyl-1-(5-aryl-1,3,4-oxadiazol-2-yl)-1-(1H-pyrrol-2-yl)methanamines were synthesi... more A series of N-benzyl-1-(5-aryl-1,3,4-oxadiazol-2-yl)-1-(1H-pyrrol-2-yl)methanamines were synthesized via one-pot reaction of appropriate benzylamine, pyrrole-2-carbaldehyde, (N-isocyanimino)triphenylphosphorane, and a carboxylic acid. The anti-tumor potential of title compounds was tested against several cancer cell lines by using MTT assay. Some tested compounds including 5e, 5p and 5q exhibited comparable or better cytotoxic activity against A549, HT29 or HT1080 cells in comparison to the reference drug doxorubicin. Also, the cytotoxic activity of compounds 5d and 5n against MCF-7 was better than that of doxorubicin. Compound 5n with IC 50 value of 4.3 mM was 4-fold more potent than doxorubicin. The structureeactivity relationship study revealed that the introduction of halogen atoms on both 5-phenyl ring and N-benzyl part improved the cytotoxic activity against all tested cell lines.
Biotechnology and Applied Biochemistry, 2014
In the present study, Fe 3 O 4 magnetic nanoparticles were synthesized by the coprecipitation of ... more In the present study, Fe 3 O 4 magnetic nanoparticles were synthesized by the coprecipitation of Fe 2+ and Fe 3+ ions and used as a nanocarrier for the production of piroctoneolamine-loaded Fe 3 O 4 nanoparticles (Fe 3 O 4 @PO NPs). The nanocrystalline structure of the prepared iron oxide species was confirmed by the X-ray diffraction spectroscopy method. Particle size distribution analysis showed that the size of Fe 3 O 4 @PO NPs was in the range of 5-55 nm. The magnetization curve of Fe 3 O 4 @PO NPs (with saturation magnetization of 28.2 emu/g) confirmed its ferromagnetic property. Loading of PO on the surface of Fe 3 O 4 NPs qualitatively verified by Fourier transform infrared spectrum obtained from Fe 3 O 4 @PO NPs. Cytotoxicity studies on the human fibrosarcoma cell line (HT-1080) revealed higher inhibitory effect of Fe 3 O 4 @PO NPs (50% cell death [IC 50 ] of 8.1 µg/mL) as compared with Fe 3 O 4 NPs (IC 50 of 117.1 µg/mL) and PO (IC 50 of 71.2 µg/mL) alone. In the case of human normal fibroblast (Hs68), the viability percentage was found to be 75% in the presence of Fe 3 O 4 @PO NPs (120 µg/mL). Gelatin zymography showed 17.2% and 34.6% inhibition of matrix metalloproteinase-2 (MMP-2) in the presence of Fe 3 O 4 @PO and PO, respectively, at the same concentration of 40 µg/mL, whereas Fe 3 O 4 NPs did not inhibit MMP-2 at any concentration.
Current Clinical Pharmacology, 2019
Background: Serotonin 5-HT3 receptor antagonists such as ondansetron have been investigated to at... more Background: Serotonin 5-HT3 receptor antagonists such as ondansetron have been investigated to attenuate opioid withdrawal signs in studies. Objective: Therefore, we designed a randomized double-blinded placebo-controlled trial to evaluate this effect on opioid-addicted patients who were admitted to the orthopedic department for surgery due to bone fractures. Methods: Male adults who were addicted to opioids, aged 18 to 79 years were enrolled (n=96) and randomized into intravenous doses (4 & 8 mg) of ondansetron (n=32) and placebo (n=32). The vital signs, withdrawal symptoms and the frequency requirement of fentanyl were recorded during anesthesia, and opioid (pethidine) analgesic was received during the recovery period. Outcome parameters were analyzed for reduction of withdrawal symptoms in addicted adults. Results: We indicated that ondansetron demonstrated significant differences with few vital outcomes including systolic blood pressure (SBP) 20 (SBP3) and 50 min (SBP4) after in...
Materials Research Bulletin, 2018
Cytotoxic and antioxidant activity of the biogenic bismuth nanoparticles produced by Delftia sp.
Biocatalysis and Agricultural Biotechnology, 2018
, Cytotoxic and antibacterial activities of biologically synthesized gold nanoparticles assisted ... more , Cytotoxic and antibacterial activities of biologically synthesized gold nanoparticles assisted by Micrococcus yunnanensis strain J2, Biocatalysis and Agricultural Biotechnology,
IET Nanobiotechnology, 2018
In this study, we evaluated the cytotoxicity underlying mechanisms of biogenic tellurium (Te) nan... more In this study, we evaluated the cytotoxicity underlying mechanisms of biogenic tellurium (Te) nanorods (NRs) produced by the Pseudomonas pseudoalcaligenes strain Te on the PC12 cell line. The half-maximal inhibitory concentration (IC50) value was estimated at 5.05±0.07 ng/ml for biogenic Te NRs and 2.44±0.38 ng/ml for K2TeO3, respectively. The viability of PC12 was inhibited concentration-dependent at doses of 1, 2.5, 5, 10, and 20 ng/ml. Te NRs principally induced late apoptosis or necrosis at IC50 concentration, without effect on caspase-3 activities. Furthermore, Te NRs reduced glutathione (GSH) and enhanced malondialdehyde (MDA) levels, and also reduced superoxide dismutase (SOD) and catalase (CAT) activities. These findings revealed that biogenic Te NRs were less toxic than K2TeO3. Additionally, they induced cytotoxity toward the PC12 cell line through the activation of late apoptosis independent of the caspase pathway, and may also enhance oxidative stress (OS) in the nervous system.
IET nanobiotechnology, 2018
This study was purposed to examine the cytotoxicity and functions of biologically synthesised bis... more This study was purposed to examine the cytotoxicity and functions of biologically synthesised bismuth nanoparticles (Bi NPs) produced by sp. SFG on human colon adenocarcinoma cell line of HT-29. The structural properties of Bi NPs were investigated using transmission electron microscopy, energy dispersive X-ray, and X-ray diffraction techniques. The cytotoxic effects of Bi NPs were analysed using flow cytometry cell apoptosis while western blot analyses were applied to analyse the cleaved caspase-3 expression. Oxidative stress (OS) damage was determined using the measurement of the glutathione (GSH) and malondialdehyde (MDA) levels and antioxidant activity of superoxide dismutase (SOD) and catalase (CAT) levels. The half maximal inhibitory concentration (IC) value of Bi NPs was measured to be 28.7 ± 1.4 µg/ml on HT-29 cell line. The viability of HT-29 represented a concentration-dependent pattern (5-80 µg/ml). The mode of Bi NPs induced apoptosis was found to be mainly related to la...
European journal of medicinal chemistry, Jan 25, 2018
A novel series of coumarin-lipoic acid conjugates were synthesized via cycloaddition click reacti... more A novel series of coumarin-lipoic acid conjugates were synthesized via cycloaddition click reaction to find out new multi-target-directed ligands (MTDLs) for treatment of Alzheimer's disease (AD). All of synthesized compounds were screened for neuroprotective and anti-cholinesterase activities. Based on primary screening, two compounds (5 and 11) were subjected to further biological evaluations. In particular, compound 11 which was the most potent AChE inhibitor showed good inhibitory effect on Aβ-aggregation and intracellular ROS (reactive oxygen species) formation, as well as the ability of selective bio-metal chelation and neuroprotection against HO- and Aβ-induced cytotoxicity. In the light of these results, the applied hybridization approach introduced new promising lead compound with desired multifunctional properties, being useful in the treatment of Alzheimer's disease.
European Journal of Medicinal Chemistry, 2017
A series of tacrine-like compounds 7a-u were designed and synthesized as potent AChE inhibitors w... more A series of tacrine-like compounds 7a-u were designed and synthesized as potent AChE inhibitors with high selectivity, low toxicity on hepatocytes, neuroprotective and and βamyloid aggregation inhibitory activity.
Journal of Trace Elements in Medicine and Biology, 2017
A lactobacillus strain (L. brevis LSe tolerates 401.3 mg L-1 of SeO3 2-) was isolated. The pe... more A lactobacillus strain (L. brevis LSe tolerates 401.3 mg L-1 of SeO3 2-) was isolated. The performed probiotic tests revealed acceptable probiotic characteristics. DPPH assay represented moderate antioxidant activity of the Se-enriched L. brevis.
Journal of Trace Elements in Medicine and Biology, 2017
The present study was designed for microwave assisted synthesis of zinc nanoparticles (Zn NPs) us... more The present study was designed for microwave assisted synthesis of zinc nanoparticles (Zn NPs) using Lavandula vera leaf extract in the presence of ZnSO 4 (1 mM). The biogenic Zn NPs were then characterized using scanning electron microscopy (SEM), energy dispersive X-ray (EDX), X-ray diffraction spectroscopy (XRD), UV-visible spectroscopy, and Fourier transform infrared spectroscopy (FTIR) techniques. Thereafter, the cytotoxic effect of ZnSO 4 and Zn NPs on different cell lines was investigated by MTT-based cytotoxicity assay and their antioxidant properties were assessed using DPPH scavenging activity and reducing power assay. The SEM micrograph showed that the Zn NPs had spherical shape with the size range of 30-80 nm. For A549, MCF-7, HT-29, and Caco-2 cell lines treated with Zn NPs, the concentration necessary causing 50% cell death (IC 50) was found to be 22.3 ± 1.1 g mL −1 , 86 ± 3.7 g mL −1 , 10.9 ± 0.5 g mL −1 , and 56.2 ± 2.8 g mL −1 , respectively. In the case of ZnSO 4 , the same results (IC 50) were observed at concentration of 81.6 ± 1.3 g mL −1 (A549), 121.0 ± 2.4 g mL −1 (MCF-7), 43.0 ± 1.4 g mL −1 (HT-29), and 85.7 ± 2.3 g mL −1 (Caco-2). The obtained results of antioxidant activity showed that the IC 50 values of butylated hydroxyanisole (BHA) and Zn NPs were 44 g mL −1 and 65.3 g mL −1 , respectively, while ZnSO 4 at concentration of 200 g mL −1 exhibited only 10.9% DPPH radical scavenging effect. Moreover, the reducing power of Zn NPs and BHA were significantly higher than ZnSO 4 (p < 0.05). To sum up, application of L. vera leaf extract combined with microwave heating energy led to simple and fast formation of Zn nanostructures exhibited higher antioxidant and cytotoxic activity compared to soluble Zn +2 ions. However, identification of the related mechanisms merit further studies.
Materials Science and Engineering: C, 2015
European Journal of Medicinal Chemistry, 2014
A series of N-benzyl-1-(5-aryl-1,3,4-oxadiazol-2-yl)-1-(1H-pyrrol-2-yl)methanamines were synthesi... more A series of N-benzyl-1-(5-aryl-1,3,4-oxadiazol-2-yl)-1-(1H-pyrrol-2-yl)methanamines were synthesized via one-pot reaction of appropriate benzylamine, pyrrole-2-carbaldehyde, (N-isocyanimino)triphenylphosphorane, and a carboxylic acid. The anti-tumor potential of title compounds was tested against several cancer cell lines by using MTT assay. Some tested compounds including 5e, 5p and 5q exhibited comparable or better cytotoxic activity against A549, HT29 or HT1080 cells in comparison to the reference drug doxorubicin. Also, the cytotoxic activity of compounds 5d and 5n against MCF-7 was better than that of doxorubicin. Compound 5n with IC 50 value of 4.3 mM was 4-fold more potent than doxorubicin. The structureeactivity relationship study revealed that the introduction of halogen atoms on both 5-phenyl ring and N-benzyl part improved the cytotoxic activity against all tested cell lines.
Biotechnology and Applied Biochemistry, 2014
In the present study, Fe 3 O 4 magnetic nanoparticles were synthesized by the coprecipitation of ... more In the present study, Fe 3 O 4 magnetic nanoparticles were synthesized by the coprecipitation of Fe 2+ and Fe 3+ ions and used as a nanocarrier for the production of piroctoneolamine-loaded Fe 3 O 4 nanoparticles (Fe 3 O 4 @PO NPs). The nanocrystalline structure of the prepared iron oxide species was confirmed by the X-ray diffraction spectroscopy method. Particle size distribution analysis showed that the size of Fe 3 O 4 @PO NPs was in the range of 5-55 nm. The magnetization curve of Fe 3 O 4 @PO NPs (with saturation magnetization of 28.2 emu/g) confirmed its ferromagnetic property. Loading of PO on the surface of Fe 3 O 4 NPs qualitatively verified by Fourier transform infrared spectrum obtained from Fe 3 O 4 @PO NPs. Cytotoxicity studies on the human fibrosarcoma cell line (HT-1080) revealed higher inhibitory effect of Fe 3 O 4 @PO NPs (50% cell death [IC 50 ] of 8.1 µg/mL) as compared with Fe 3 O 4 NPs (IC 50 of 117.1 µg/mL) and PO (IC 50 of 71.2 µg/mL) alone. In the case of human normal fibroblast (Hs68), the viability percentage was found to be 75% in the presence of Fe 3 O 4 @PO NPs (120 µg/mL). Gelatin zymography showed 17.2% and 34.6% inhibition of matrix metalloproteinase-2 (MMP-2) in the presence of Fe 3 O 4 @PO and PO, respectively, at the same concentration of 40 µg/mL, whereas Fe 3 O 4 NPs did not inhibit MMP-2 at any concentration.