milan seman - Academia.edu (original) (raw)
Papers by milan seman
Folia Microbiologica, Nov 1, 2004
The resistance to antibiotics and the distribution of virulence factors in enterococci isolated f... more The resistance to antibiotics and the distribution of virulence factors in enterococci isolated from traditional Slovak sheep cheese bryndza was compared with strains from human infections. The occurrence of 4 enterococcal species was observed in 117 bryndza-cheese isolates. The majority of strains were identified as E. faecium (76 %) and E. faecalis (23 %). Several strains of E. durans and 1 strain of E. hirae were also present. More than 90 % of strains isolated from 109 clinical enterococci were E. faecalis, the rest belonged to E. faecium. The resistance to 6 antimicrobial substances (ampicillin, ciprofloxacin, higher concentration of gentamicin, nitrofurantoin, tetracycline and vancomycin) was tested in clinical and food enterococci. A higher level of resistance was found in clinical than in food strains and E. faecium had a higher resistance than E. faecalis; no resistance to vancomycin was detected. The occurrence of 3 virulence-associated genes, cylA (coding for hemolysin), gelE (coding for gelatinase) and esp (coding for surface protein) was monitored. Differences were found in the distribution of cylA gene between clinical and bryndza-cheese E. faecalis strains; in contrast to clinical strains (45 %), cylA gene was detected in 22 % of food isolates. The distribution of 2 other virulence factors, gelE and esp, was not significantly different in the two groups of E. faecalis strains. cylA and gelE genes were not detected in E. faecium but more than 70 % of clinical E. faecium were positive for esp, even thought none of the 79 E. faecium cheese isolates contained this gene.
PubMed, Mar 1, 1997
The paper summarizes at a subspecific level 11 clinical strains of K. pneumoniae. The objective o... more The paper summarizes at a subspecific level 11 clinical strains of K. pneumoniae. The objective of the work was to determine in a simple and effective way differences between different strains of the mentioned taxon. Biochemical characteristics, antibiogram and part of the plasmid spectrum were used for assessment of inter-species differences between different strains and at the same time their use as simple markers of epidemiological analyses is presented.
Fems Microbiology Letters, Jun 1, 2005
A set of reference strains and a group of previously unidentified enterococci were analysed by re... more A set of reference strains and a group of previously unidentified enterococci were analysed by rep-PCR with the (GTG) 5 primer to evaluate the discriminatory power and suitability of this method for typing and identification of enterococcal species. A total of 49 strains representing all validly described species were obtained from bacterial collections. For more extensive evaluation of this identification approach 112 well-defined and identified enterococci isolated from bryndza cheese were tested. The (GTG) 5-PCR fingerprinting assigned all strains into well-differentiated clusters representing individual species. Subsequently, a group including 44 unidentified enterococci isolated from surface waters was analysed to evaluate this method for identification of unknown isolates. Obtained band patterns allowed us to identify all the strains clearly to the species level. This study proved that rep-PCR with (GTG) 5 primer is a reliable and fast method for species identification of enterococci.
Mutation research, Mar 1, 1983
PubMed, Jun 1, 1993
Background: Yersinia enterocolitica is a human pathogen which an acts on the intestine by basical... more Background: Yersinia enterocolitica is a human pathogen which an acts on the intestine by basically enteroinvasive mechanism. Its virulence has been related with the presence of a plasmid of 40-50 MD which codes a series of properties. There are strains of Y. enterocolitica and of other species assimilated to the Y. enterocolitica group which lack the virulence plasmid. In these cases there is a problem in evaluating the pathogenic ability on the intestine of these bacterias when isolated in faeces. Methods: A study of 30 Yersinia spp. strains including growth in a magnesium oxalate medium, Reg-Congo and agar (CR-MOX), sculine hydrolisis (Sc), pyrazinamidase activity (Pyz) and salicine fermentation (Sal) was performed. In addition, the presence of virulence plasmid (VP) was determined. Results: Twenty-two strains identified as Y. enterocolitica presented the virulence pattern (CR-MOX+, Pyz-, Sal/Sc-) and 21 were VP+. Seven strains isolated were CR-MOX-, Pyz+, Sal/Sc+ and VP- being typed as Y. fredericksenii (6) and Y. kristensenii (1). The remaining strain was CR-MOX- and VP- but Pyz, Sal/Sc were also negative, being identified as Y. enterocolitica. Conclusions: By the tests referred the authors were able to identify and evaluate the pathogenicity of Yersinia spp. isolated in faeces. These techniques may be used in the microbiology laboratory as a method which aids to evaluate the diagnosis of intestinal infections caused by Yersinia spp.
Serratia spp. kmeně izolovane z jaskynných vod byly charakterizovany biochemicky a pomocou SDS-PA... more Serratia spp. kmeně izolovane z jaskynných vod byly charakterizovany biochemicky a pomocou SDS-PAGE. Vybrana skupina izolatov byla identifikovana sekvencnou analýzou (16S rRNA, rpoB a recN gene).
Mikrobiologicka analýza siestich vzoriek makkeho sintra z troch jaskýň Slovenska prokazala bohatý... more Mikrobiologicka analýza siestich vzoriek makkeho sintra z troch jaskýň Slovenska prokazala bohatý výskyt psychrotrofných bakterii a mikroskopických hůb.
A group of previously well-identified enterococci isolated from a typical Slovakian sheep-milk pr... more A group of previously well-identified enterococci isolated from a typical Slovakian sheep-milk product bryndza were characterized by the (GTG)5-PCR fingerprinting in order to evaluate discriminatory power of this method for identification and strain-typing. Analysed group consisted of E. faecium (n = 76), E. faecalis (n = 25) and E. durans (n = 10) strains. They were sampled from two bryndza-processing manufactories during three monthly samplings performed successively (April, May and June) in 2000 and 2001. Isolated strains were characterized and identified by biotyping, ITS-PCR, ddlE.faecalis and ddlE.faecium PCRs. Species identity of representative strains selected from each (GTG)5-PCR fingerprint subcluster was confirmed by SDS-PAGE of whole-cell proteins. The (GTG)5-PCR fingerprint profiles obtained from E. faecalis strains were visually very close. Similarly, E. durans strains revealed nearly identical fingerprints. E. faecium strains were separated into two clearly separated ...
Detailed chemical and microbiological research was realised in the Krásnohorská Cave and its unde... more Detailed chemical and microbiological research was realised in the Krásnohorská Cave and its underground hydrological system (2009 – 2011). Predominant part of the cave is formed in Gutenstein dolomite and dolomite limestones, rear parts of the cave are located in chemically pure Steinalm limestone of the Silicic Unit. Chemical composition, quality and microbiological properties of karst water in the cave system were overall evaluated on the basis of 55 chemical and 41 microbiological analyses and results of continual measurement of electric conductivity and water temperature. The chemical composition of water is relatively stable during year. Carbonates and calcium are dominant ions in water and is formed water with carbonate mineralization. Values of the total dissolved solids (TDS) are from 488 to 1,097 mg·L. Cave water is primary Ca-HCO3 and Ca-Mg-HCO3 type, but locally Ca-SO4 type is found as well. Results showed diff erences between some parts of cave system and confi rmed occ...
Z krasových vod vybraných jaskýň Slovenskeho krasu (Domica, Milada, Gombasecka jaskyňa, Krasnohor... more Z krasových vod vybraných jaskýň Slovenskeho krasu (Domica, Milada, Gombasecka jaskyňa, Krasnohorska jaskyňa) bolo v rokoch 2007 – 2011 ziskaných 800 izolatov klasifikovaných ako enterobakterie. Dominantnu zložku tvori rod Serratia s druhmi: S. fonticola, S. marcescens, S. plymuthica, S.odorifera, S. grimesii, S. quinivora, S. liquefaciens v pocte asi 400 izolatov.
Chemischer Informationsdienst, 1983
ChemInform Abstract Das bekannte Z-Isomere bzw. das E-Isomere des Furylvinylbromids (II), letzter... more ChemInform Abstract Das bekannte Z-Isomere bzw. das E-Isomere des Furylvinylbromids (II), letzteres dargestellt aus dem Furylacrylat (I), dienen zur Quaternisierung der Pyridine (IV) zu den entsprechenden Isomeren der Pyridiniumsalze (V). Wird die Reaktion in polaren Lösungsmitteln bzw. bei Temp.über 100 rc C durchgeführt, wird cis-trans-Isomerisierung beobachtet. Die analogen Umsetzungen mit den stärker aciden Cyanpyridinen gelingen nicht. Bromierung von (Va) führt zum Salz (VI), das am Beispiel mit Aceton (VII) als ochreaktives Bromierungsreagenz eingesetzt wird. Auch hier tritt bei entsprechender Reaktionsführung eine Isomerisierung der Z-Isomeren von (VI) bzw. (Va) auf. Salze von (Va) besitzen starke antibakterielle und fungicide Wirkung sowie hohe biologische Aktivität gegen almonellen. Methylsubstituenten am Pyridinring vermindern die biologische Wirksamkeit.
Detailed chemical and microbiological research was realised in the Krásnohorská Cave and its unde... more Detailed chemical and microbiological research was realised in the Krásnohorská Cave and its underground hydrological system (2009 – 2011). Predominant part of the cave is formed in Gutenstein dolomite and dolomite limestones, rear parts of the cave are located in chemically pure Steinalm limestone of the Silicic Unit. Chemical composition, quality and microbiological properties of karst water in the cave system were overall evaluated on the basis of 55 chemical and 41 microbiological analyses and results of continual measurement of electric conductivity and water temperature. The chemical composition of water is relatively stable during year. Carbonates and calcium are dominant ions in water and is formed water with carbonate mineralization. Values of the total dissolved solids (TDS) are from 488 to 1,097 mg·L-1. Cave water is primary Ca-HCO3 and Ca-Mg-HCO3 type, but locally Ca-SO4 type is found as well. Results showed diff erences between some parts of cave system and confi rmed o...
International Aquatic Research, 2014
The gravel pits are typical features of Bratislava. By their origin they are related to the river... more The gravel pits are typical features of Bratislava. By their origin they are related to the river Danube. The water quality is determinated by various indicators, especially hydrochemical and microbiological ones. In the gravel pits water was determined for increase of chloride concentration and drop of sulphate concentration. Significant indicator of faecal contamination is the presence of enterococci. Faecal enterococci (E. faecalis, E. faecium, E. durans, E. hirae) dominates in the river Danube, and only two nonfaecal enterococcal species, E. casseliflavus and E. seriolicida were found in the water of gravel pits with other enterococcal very related taxa: Lactococcus lactis and Aeromonas spp.
International Journal of Speleology, 2014
in show caves (Bastian et al., 2009). An important factor affecting protected locations is buildi... more in show caves (Bastian et al., 2009). An important factor affecting protected locations is building villages or animal farms nearby which may be a source of pollution (Martinez, 2009). The karst system is extremely sensitive to infiltration with polluted water contaminated by bacteria or pharmacologically active substances (Brandon et al., 2010; Einsiedl et al., 2010; Morasch, 2013). Escherichia coli and total coliform bacteria including members of the genus Enterobacter are conventional indicators of microbial contamination (WHO, 2006). Antibiotics used in prevention and treatment of animal diseases, and to increase animal growth have still been one of the major factors of pollution despite the recommendation of the European Union on the prudent use of antibiotics and on a coordination between human and veterinary antibiotic policy (EFSA, 2010; Obeng et al., 2012; Harnisz, 2013). Prevalence of antibiotics in agriculture may result in violation of ecosystem with an impact to micro-and macrobiota located in these conserved biotopes due to a dissemination of bacteria carrying
Monatshefte für Chemie - Chemical Monthly, 1995
Catalytic hydrogenation of 2,3-diphenyl-6-nitroquinoxaline led to the corresponding amilae 1 whic... more Catalytic hydrogenation of 2,3-diphenyl-6-nitroquinoxaline led to the corresponding amilae 1 which in turn afforded products 3a-i on reaction with alkoxymethylene derivatives 2a-i. Thermal cyclization of 3b and 3f yielded substituted pyrazinoquinolones 5b and 5f, respectively. Optimal conditions for the successful hydrolysis of ester 5b were found. The structures of all products were deduced from their IR, UV, 1H, and 13C NMR spectroscopic data.
Folia Microbiologica, Nov 1, 2004
The resistance to antibiotics and the distribution of virulence factors in enterococci isolated f... more The resistance to antibiotics and the distribution of virulence factors in enterococci isolated from traditional Slovak sheep cheese bryndza was compared with strains from human infections. The occurrence of 4 enterococcal species was observed in 117 bryndza-cheese isolates. The majority of strains were identified as E. faecium (76 %) and E. faecalis (23 %). Several strains of E. durans and 1 strain of E. hirae were also present. More than 90 % of strains isolated from 109 clinical enterococci were E. faecalis, the rest belonged to E. faecium. The resistance to 6 antimicrobial substances (ampicillin, ciprofloxacin, higher concentration of gentamicin, nitrofurantoin, tetracycline and vancomycin) was tested in clinical and food enterococci. A higher level of resistance was found in clinical than in food strains and E. faecium had a higher resistance than E. faecalis; no resistance to vancomycin was detected. The occurrence of 3 virulence-associated genes, cylA (coding for hemolysin), gelE (coding for gelatinase) and esp (coding for surface protein) was monitored. Differences were found in the distribution of cylA gene between clinical and bryndza-cheese E. faecalis strains; in contrast to clinical strains (45 %), cylA gene was detected in 22 % of food isolates. The distribution of 2 other virulence factors, gelE and esp, was not significantly different in the two groups of E. faecalis strains. cylA and gelE genes were not detected in E. faecium but more than 70 % of clinical E. faecium were positive for esp, even thought none of the 79 E. faecium cheese isolates contained this gene.
PubMed, Mar 1, 1997
The paper summarizes at a subspecific level 11 clinical strains of K. pneumoniae. The objective o... more The paper summarizes at a subspecific level 11 clinical strains of K. pneumoniae. The objective of the work was to determine in a simple and effective way differences between different strains of the mentioned taxon. Biochemical characteristics, antibiogram and part of the plasmid spectrum were used for assessment of inter-species differences between different strains and at the same time their use as simple markers of epidemiological analyses is presented.
Fems Microbiology Letters, Jun 1, 2005
A set of reference strains and a group of previously unidentified enterococci were analysed by re... more A set of reference strains and a group of previously unidentified enterococci were analysed by rep-PCR with the (GTG) 5 primer to evaluate the discriminatory power and suitability of this method for typing and identification of enterococcal species. A total of 49 strains representing all validly described species were obtained from bacterial collections. For more extensive evaluation of this identification approach 112 well-defined and identified enterococci isolated from bryndza cheese were tested. The (GTG) 5-PCR fingerprinting assigned all strains into well-differentiated clusters representing individual species. Subsequently, a group including 44 unidentified enterococci isolated from surface waters was analysed to evaluate this method for identification of unknown isolates. Obtained band patterns allowed us to identify all the strains clearly to the species level. This study proved that rep-PCR with (GTG) 5 primer is a reliable and fast method for species identification of enterococci.
Mutation research, Mar 1, 1983
PubMed, Jun 1, 1993
Background: Yersinia enterocolitica is a human pathogen which an acts on the intestine by basical... more Background: Yersinia enterocolitica is a human pathogen which an acts on the intestine by basically enteroinvasive mechanism. Its virulence has been related with the presence of a plasmid of 40-50 MD which codes a series of properties. There are strains of Y. enterocolitica and of other species assimilated to the Y. enterocolitica group which lack the virulence plasmid. In these cases there is a problem in evaluating the pathogenic ability on the intestine of these bacterias when isolated in faeces. Methods: A study of 30 Yersinia spp. strains including growth in a magnesium oxalate medium, Reg-Congo and agar (CR-MOX), sculine hydrolisis (Sc), pyrazinamidase activity (Pyz) and salicine fermentation (Sal) was performed. In addition, the presence of virulence plasmid (VP) was determined. Results: Twenty-two strains identified as Y. enterocolitica presented the virulence pattern (CR-MOX+, Pyz-, Sal/Sc-) and 21 were VP+. Seven strains isolated were CR-MOX-, Pyz+, Sal/Sc+ and VP- being typed as Y. fredericksenii (6) and Y. kristensenii (1). The remaining strain was CR-MOX- and VP- but Pyz, Sal/Sc were also negative, being identified as Y. enterocolitica. Conclusions: By the tests referred the authors were able to identify and evaluate the pathogenicity of Yersinia spp. isolated in faeces. These techniques may be used in the microbiology laboratory as a method which aids to evaluate the diagnosis of intestinal infections caused by Yersinia spp.
Serratia spp. kmeně izolovane z jaskynných vod byly charakterizovany biochemicky a pomocou SDS-PA... more Serratia spp. kmeně izolovane z jaskynných vod byly charakterizovany biochemicky a pomocou SDS-PAGE. Vybrana skupina izolatov byla identifikovana sekvencnou analýzou (16S rRNA, rpoB a recN gene).
Mikrobiologicka analýza siestich vzoriek makkeho sintra z troch jaskýň Slovenska prokazala bohatý... more Mikrobiologicka analýza siestich vzoriek makkeho sintra z troch jaskýň Slovenska prokazala bohatý výskyt psychrotrofných bakterii a mikroskopických hůb.
A group of previously well-identified enterococci isolated from a typical Slovakian sheep-milk pr... more A group of previously well-identified enterococci isolated from a typical Slovakian sheep-milk product bryndza were characterized by the (GTG)5-PCR fingerprinting in order to evaluate discriminatory power of this method for identification and strain-typing. Analysed group consisted of E. faecium (n = 76), E. faecalis (n = 25) and E. durans (n = 10) strains. They were sampled from two bryndza-processing manufactories during three monthly samplings performed successively (April, May and June) in 2000 and 2001. Isolated strains were characterized and identified by biotyping, ITS-PCR, ddlE.faecalis and ddlE.faecium PCRs. Species identity of representative strains selected from each (GTG)5-PCR fingerprint subcluster was confirmed by SDS-PAGE of whole-cell proteins. The (GTG)5-PCR fingerprint profiles obtained from E. faecalis strains were visually very close. Similarly, E. durans strains revealed nearly identical fingerprints. E. faecium strains were separated into two clearly separated ...
Detailed chemical and microbiological research was realised in the Krásnohorská Cave and its unde... more Detailed chemical and microbiological research was realised in the Krásnohorská Cave and its underground hydrological system (2009 – 2011). Predominant part of the cave is formed in Gutenstein dolomite and dolomite limestones, rear parts of the cave are located in chemically pure Steinalm limestone of the Silicic Unit. Chemical composition, quality and microbiological properties of karst water in the cave system were overall evaluated on the basis of 55 chemical and 41 microbiological analyses and results of continual measurement of electric conductivity and water temperature. The chemical composition of water is relatively stable during year. Carbonates and calcium are dominant ions in water and is formed water with carbonate mineralization. Values of the total dissolved solids (TDS) are from 488 to 1,097 mg·L. Cave water is primary Ca-HCO3 and Ca-Mg-HCO3 type, but locally Ca-SO4 type is found as well. Results showed diff erences between some parts of cave system and confi rmed occ...
Z krasových vod vybraných jaskýň Slovenskeho krasu (Domica, Milada, Gombasecka jaskyňa, Krasnohor... more Z krasových vod vybraných jaskýň Slovenskeho krasu (Domica, Milada, Gombasecka jaskyňa, Krasnohorska jaskyňa) bolo v rokoch 2007 – 2011 ziskaných 800 izolatov klasifikovaných ako enterobakterie. Dominantnu zložku tvori rod Serratia s druhmi: S. fonticola, S. marcescens, S. plymuthica, S.odorifera, S. grimesii, S. quinivora, S. liquefaciens v pocte asi 400 izolatov.
Chemischer Informationsdienst, 1983
ChemInform Abstract Das bekannte Z-Isomere bzw. das E-Isomere des Furylvinylbromids (II), letzter... more ChemInform Abstract Das bekannte Z-Isomere bzw. das E-Isomere des Furylvinylbromids (II), letzteres dargestellt aus dem Furylacrylat (I), dienen zur Quaternisierung der Pyridine (IV) zu den entsprechenden Isomeren der Pyridiniumsalze (V). Wird die Reaktion in polaren Lösungsmitteln bzw. bei Temp.über 100 rc C durchgeführt, wird cis-trans-Isomerisierung beobachtet. Die analogen Umsetzungen mit den stärker aciden Cyanpyridinen gelingen nicht. Bromierung von (Va) führt zum Salz (VI), das am Beispiel mit Aceton (VII) als ochreaktives Bromierungsreagenz eingesetzt wird. Auch hier tritt bei entsprechender Reaktionsführung eine Isomerisierung der Z-Isomeren von (VI) bzw. (Va) auf. Salze von (Va) besitzen starke antibakterielle und fungicide Wirkung sowie hohe biologische Aktivität gegen almonellen. Methylsubstituenten am Pyridinring vermindern die biologische Wirksamkeit.
Detailed chemical and microbiological research was realised in the Krásnohorská Cave and its unde... more Detailed chemical and microbiological research was realised in the Krásnohorská Cave and its underground hydrological system (2009 – 2011). Predominant part of the cave is formed in Gutenstein dolomite and dolomite limestones, rear parts of the cave are located in chemically pure Steinalm limestone of the Silicic Unit. Chemical composition, quality and microbiological properties of karst water in the cave system were overall evaluated on the basis of 55 chemical and 41 microbiological analyses and results of continual measurement of electric conductivity and water temperature. The chemical composition of water is relatively stable during year. Carbonates and calcium are dominant ions in water and is formed water with carbonate mineralization. Values of the total dissolved solids (TDS) are from 488 to 1,097 mg·L-1. Cave water is primary Ca-HCO3 and Ca-Mg-HCO3 type, but locally Ca-SO4 type is found as well. Results showed diff erences between some parts of cave system and confi rmed o...
International Aquatic Research, 2014
The gravel pits are typical features of Bratislava. By their origin they are related to the river... more The gravel pits are typical features of Bratislava. By their origin they are related to the river Danube. The water quality is determinated by various indicators, especially hydrochemical and microbiological ones. In the gravel pits water was determined for increase of chloride concentration and drop of sulphate concentration. Significant indicator of faecal contamination is the presence of enterococci. Faecal enterococci (E. faecalis, E. faecium, E. durans, E. hirae) dominates in the river Danube, and only two nonfaecal enterococcal species, E. casseliflavus and E. seriolicida were found in the water of gravel pits with other enterococcal very related taxa: Lactococcus lactis and Aeromonas spp.
International Journal of Speleology, 2014
in show caves (Bastian et al., 2009). An important factor affecting protected locations is buildi... more in show caves (Bastian et al., 2009). An important factor affecting protected locations is building villages or animal farms nearby which may be a source of pollution (Martinez, 2009). The karst system is extremely sensitive to infiltration with polluted water contaminated by bacteria or pharmacologically active substances (Brandon et al., 2010; Einsiedl et al., 2010; Morasch, 2013). Escherichia coli and total coliform bacteria including members of the genus Enterobacter are conventional indicators of microbial contamination (WHO, 2006). Antibiotics used in prevention and treatment of animal diseases, and to increase animal growth have still been one of the major factors of pollution despite the recommendation of the European Union on the prudent use of antibiotics and on a coordination between human and veterinary antibiotic policy (EFSA, 2010; Obeng et al., 2012; Harnisz, 2013). Prevalence of antibiotics in agriculture may result in violation of ecosystem with an impact to micro-and macrobiota located in these conserved biotopes due to a dissemination of bacteria carrying
Monatshefte für Chemie - Chemical Monthly, 1995
Catalytic hydrogenation of 2,3-diphenyl-6-nitroquinoxaline led to the corresponding amilae 1 whic... more Catalytic hydrogenation of 2,3-diphenyl-6-nitroquinoxaline led to the corresponding amilae 1 which in turn afforded products 3a-i on reaction with alkoxymethylene derivatives 2a-i. Thermal cyclization of 3b and 3f yielded substituted pyrazinoquinolones 5b and 5f, respectively. Optimal conditions for the successful hydrolysis of ester 5b were found. The structures of all products were deduced from their IR, UV, 1H, and 13C NMR spectroscopic data.