willan muñoz - Academia.edu (original) (raw)
Drafts by willan muñoz
CEDAMAZ REVISTA UNL, 2017
Newcastle disease virus is a highly conta-gious avian paramyxovirus and the main de-v... more Newcastle disease virus is a highly conta-gious avian paramyxovirus and the main de-vastating poultry agent, causing high econo-mic losses in the poultry industry around the world. In the present study, we characterized molecularly 8 partial gene sequences of the fu-sion protein and 3 complete sequences of the haemagglutinin-neuraminidase from isolated virus present in native poultry unvaccinated in the Southern Ecuador in 2014. These sequences were compared with other sequences of virus, representing different genotypes and sub-ge-notypes of virus from different regions of the world. All viruses showed the amino acid se-quence 112GRQGRL117 in C-terminus extension of the F2 protein, and showed Leucine at the N-terminus extension of the F1 protein. Those sequences allowed us to determine that these viruses belonged to a type of lentogenic virus. Phylogenetic analysis showed that most of the Newcastle virus isolated in this region of Ecua-dor, were closely related to strains of genotype II Class II, consistent with the sequence lento-genic live-virus vaccine, La Sota and B1. Mole-cular characterization and phylogenetic study of the F and HN genes could reliably be used as a tool for monitoring and early detection of Newcastle disease virus, as well as to predict the different pathotypes of virus isolates.Palabras clave: Análisis filogenético; Proteína de fusión; Proteína hemaglutinina-neuraminidasa; Virus de la enfermedad de Newcastle.Keywords: Phylogenetic analysis; Fusion protein; Hemagglutinin-neuraminidase protein; Newcastle Disease Virus.INTRODUCCIÓN
Ensayo de retromutacion mediante test de Ames, 2018
En varias regiones del sur del Ecuador diferentes especies de plantas son usadas para tratar una ... more En varias regiones del sur del Ecuador diferentes especies de plantas son usadas para tratar una variedad de dolencias. Tres extractos metanólicos de plantas usadas en la medicina tradicional en la provincia de Loja, fueron investigadas con el objetivo de evaluar su posible potencial mutagénico y antimutagénico usando el ensayo de retromutación en Salmonella typhimurium (test de Ames) con las cepas bacterianas TA1535, TA100, TA1538, TA98 (ensayo de mutagenicidad), TA100 y TA98 (ensayo de antimutagenicidad) en ausencia de activación metabólica (-S9). Los extractos metanólicos de las hojas de Baccharis latifolia y Callisia repens dieron positivo para el ensayo de mutagenicidad. Ludwigia peruviana mostró propiedades antimutagénicas, contra al menos uno de los mutágenos ensayados. Estos resultados contribuyen a obtener valiosos datos sobre el uso seguro de las plantas medicinales y sus posibles efectos quimiopreventivos. Teniendo en cuenta las excelentes actividades antimutagénicas extraídas de Ludwigia peruviana estos extractos podrían ser buenas fuentes de agentes quimiopreventivos. Sin embargo, Baccharis latifolia y Callisia repens mostraron actividad mutagénica lo que sugiere precaución en su uso. La actividad mutagénica y antimutagénica de estas especies indican que son recursos valiosos para ser conservados y considerados para futuras investigaciones.
Papers by willan muñoz
DOAJ (DOAJ: Directory of Open Access Journals), Aug 1, 2020
Ioffe Institute Russian Academy of Sciences, 2018
Журнал технической физики, 2018
CEDAMAZ REVISTA UNL, 2017
Newcastle disease virus is a highly conta-gious avian paramyxovirus and the main de-vastating pou... more Newcastle disease virus is a highly conta-gious avian paramyxovirus and the main de-vastating poultry agent, causing high econo-mic losses in the poultry industry around the world. In the present study, we characterized molecularly 8 partial gene sequences of the fu-sion protein and 3 complete sequences of the haemagglutinin-neuraminidase from isolated virus present in native poultry unvaccinated in the Southern Ecuador in 2014. These sequences were compared with other sequences of virus, representing different genotypes and sub-ge-notypes of virus from different regions of the world. All viruses showed the amino acid se-quence 112GRQGRL117 in C-terminus extension of the F2 protein, and showed Leucine at the N-terminus extension of the F1 protein. Those sequences allowed us to determine that these viruses belonged to a type of lentogenic virus. Phylogenetic analysis showed that most of the Newcastle virus isolated in this region of Ecua-dor, were closely related to strains of genoty...
Newcastle disease virus is a highly contagious avian paramyxovirus and the main devastating poult... more Newcastle disease virus is a highly contagious avian paramyxovirus and the main devastating poultry agent, causing high economic losses in the poultry industry around the world. In the present study, we characterized molecularly 8 partial gene sequences of the fusion protein and 3 complete sequences of the haemagglutinin-neuraminidase from isolated virus present in native poultry unvaccinated in the Southern Ecuador in 2014. These sequences were compared with other sequences of virus, representing different genotypes and sub-genotypes of virus from different regions of the world. All viruses showed the amino acid sequence 112GRQGRL117 in C-terminus extension of the F2 protein, and showed Leucine at the N-terminus extension of the F1 protein. Those sequences allowed us to determine that these viruses belonged to a type of lentogenic virus. Phylogenetic analysis showed that most of the Newcastle virus isolated in this region of Ecuador, were closely related to strains of genotype II C...
CEDAMAZ, 2019
Resumen-En varias regiones del sur del Ecuador diferentes especies de plantas son usadas para tra... more Resumen-En varias regiones del sur del Ecuador diferentes especies de plantas son usadas para tratar una variedad de dolencias. Tres extractos metanólicos de plantas usadas en la medicina tradicional en la provincia de Loja fueron investigadas con el objetivo de evaluar su posible potencial mutagénico y antimutagénico usando el ensayo de retromutación en Salmonella typhimurium (test de Ames) con las cepas bacterianas TA1535, TA100, TA1538, TA98 (ensayo de mutagenicidad), TA100 y TA98 (ensayo de antimutagenicidad) en ausencia de activación metabólica (-S9). Los extractos metanólicos de las hojas de Baccharis latifolia y Callisia repens dieron positivo para el ensayo de mutagenicidad. Ludwigia peruviana mostró propiedades antimutagénicas, contra al menos uno de los mutágenos ensayados. Estos resultados contribuyen a obtener valiosos datos sobre el uso seguro de las plantas medicinales y sus posibles efectos quimiopreventivos. Teniendo en cuenta las excelentes actividades antimutagénicas extraídas de Ludwigia peruviana estos extractos podrían ser buenas fuentes de agentes quimiopreventivos. Sin embargo, Baccharis latifolia y Callisia repens mostraron actividad mutagénica lo que sugiere precaución en su uso. La actividad mutagénica y antimutagénica de estas especies indican que son recursos valiosos para ser conservados y considerados para futuras investigaciones. Palabras clave-Extractos metanólicos; Plantas medicinales; Test de Ames Abstract-In various regions of the South of the Ecuador different plant species are used to treat a variety of ailments. Methanol extracts from plants used in traditional medicine in the province of Loja, were investigated with the objective of evaluating their possible potential mutagenic and antimutagenic using retromutation test in Salmonella typhimurium (Ames Test) with bacterial strains TA1535, TA1538, TA98 and TA100 (mutagenicity test), TA100 and TA98 (antimutagenicity test) in the absence of metabolic activation (-S9). Methanol extracts from the leaves of Baccharis latifolia and Callisia repens were positive for mutagenicity testing. Ludwigia peruviana was found to be strongly antimutagenic againts at least one of the mutagens tested. These results contribute to valuable data on the safe use of medicinal plants and their potential chemopreventive effects. Considering the excellent antimutagenic activities extracted from Ludwigia peruviana these extracts are good candidate source of chemopreventive agent. However, Baccharis latifolia and Callisia repens showed mutagenic activity, suggesting caution in their use. The mutagenic and antimutagenic activity of these species indicates that they are valuable resources that should be conserved and considered for future research. INTRODUCCIÓN E n la última década, el uso de plantas medicinales ha aumentado sustancialmente, ya sea como agentes em-pleados en medicina tradicional y/o como material de origen para la producción de suplementos dietéticos, tanto en cul-turas occidentales como asiáticas (Sponchiado et al., 2016). Datos de contacto: Willan Muñoz-Chamba, wamunoz24@gmail.com Se sabe que el uso de plantas medicinales tradicionales pre-sentes en la dieta tiene una gran importancia farmacológi-ca, atribuida a la presencia de diversos componentes fitoquí-micos. Estos compuestos químicos presentes en las plantas transmiten sus efectos biológicos ya sea actuando exclusi-va o sinérgicamente, a través de uno o más mecanismos di-ferentes (Khan et al., 2018). El uso de plantas medicinales en la medicina popular se basa en el conocimiento empírico acumulado durante siglos por grupos étnicos (Espanha et al., 2014). Desde la antigüedad, los hombres han utilizado las plantas medicinales en el tratamiento de diferentes enferme-35
CEDAMAZ REVISTA UNL, 2017
Newcastle disease virus is a highly conta-gious avian paramyxovirus and the main de-v... more Newcastle disease virus is a highly conta-gious avian paramyxovirus and the main de-vastating poultry agent, causing high econo-mic losses in the poultry industry around the world. In the present study, we characterized molecularly 8 partial gene sequences of the fu-sion protein and 3 complete sequences of the haemagglutinin-neuraminidase from isolated virus present in native poultry unvaccinated in the Southern Ecuador in 2014. These sequences were compared with other sequences of virus, representing different genotypes and sub-ge-notypes of virus from different regions of the world. All viruses showed the amino acid se-quence 112GRQGRL117 in C-terminus extension of the F2 protein, and showed Leucine at the N-terminus extension of the F1 protein. Those sequences allowed us to determine that these viruses belonged to a type of lentogenic virus. Phylogenetic analysis showed that most of the Newcastle virus isolated in this region of Ecua-dor, were closely related to strains of genotype II Class II, consistent with the sequence lento-genic live-virus vaccine, La Sota and B1. Mole-cular characterization and phylogenetic study of the F and HN genes could reliably be used as a tool for monitoring and early detection of Newcastle disease virus, as well as to predict the different pathotypes of virus isolates.Palabras clave: Análisis filogenético; Proteína de fusión; Proteína hemaglutinina-neuraminidasa; Virus de la enfermedad de Newcastle.Keywords: Phylogenetic analysis; Fusion protein; Hemagglutinin-neuraminidase protein; Newcastle Disease Virus.INTRODUCCIÓN
Ensayo de retromutacion mediante test de Ames, 2018
En varias regiones del sur del Ecuador diferentes especies de plantas son usadas para tratar una ... more En varias regiones del sur del Ecuador diferentes especies de plantas son usadas para tratar una variedad de dolencias. Tres extractos metanólicos de plantas usadas en la medicina tradicional en la provincia de Loja, fueron investigadas con el objetivo de evaluar su posible potencial mutagénico y antimutagénico usando el ensayo de retromutación en Salmonella typhimurium (test de Ames) con las cepas bacterianas TA1535, TA100, TA1538, TA98 (ensayo de mutagenicidad), TA100 y TA98 (ensayo de antimutagenicidad) en ausencia de activación metabólica (-S9). Los extractos metanólicos de las hojas de Baccharis latifolia y Callisia repens dieron positivo para el ensayo de mutagenicidad. Ludwigia peruviana mostró propiedades antimutagénicas, contra al menos uno de los mutágenos ensayados. Estos resultados contribuyen a obtener valiosos datos sobre el uso seguro de las plantas medicinales y sus posibles efectos quimiopreventivos. Teniendo en cuenta las excelentes actividades antimutagénicas extraídas de Ludwigia peruviana estos extractos podrían ser buenas fuentes de agentes quimiopreventivos. Sin embargo, Baccharis latifolia y Callisia repens mostraron actividad mutagénica lo que sugiere precaución en su uso. La actividad mutagénica y antimutagénica de estas especies indican que son recursos valiosos para ser conservados y considerados para futuras investigaciones.
DOAJ (DOAJ: Directory of Open Access Journals), Aug 1, 2020
Ioffe Institute Russian Academy of Sciences, 2018
Журнал технической физики, 2018
CEDAMAZ REVISTA UNL, 2017
Newcastle disease virus is a highly conta-gious avian paramyxovirus and the main de-vastating pou... more Newcastle disease virus is a highly conta-gious avian paramyxovirus and the main de-vastating poultry agent, causing high econo-mic losses in the poultry industry around the world. In the present study, we characterized molecularly 8 partial gene sequences of the fu-sion protein and 3 complete sequences of the haemagglutinin-neuraminidase from isolated virus present in native poultry unvaccinated in the Southern Ecuador in 2014. These sequences were compared with other sequences of virus, representing different genotypes and sub-ge-notypes of virus from different regions of the world. All viruses showed the amino acid se-quence 112GRQGRL117 in C-terminus extension of the F2 protein, and showed Leucine at the N-terminus extension of the F1 protein. Those sequences allowed us to determine that these viruses belonged to a type of lentogenic virus. Phylogenetic analysis showed that most of the Newcastle virus isolated in this region of Ecua-dor, were closely related to strains of genoty...
Newcastle disease virus is a highly contagious avian paramyxovirus and the main devastating poult... more Newcastle disease virus is a highly contagious avian paramyxovirus and the main devastating poultry agent, causing high economic losses in the poultry industry around the world. In the present study, we characterized molecularly 8 partial gene sequences of the fusion protein and 3 complete sequences of the haemagglutinin-neuraminidase from isolated virus present in native poultry unvaccinated in the Southern Ecuador in 2014. These sequences were compared with other sequences of virus, representing different genotypes and sub-genotypes of virus from different regions of the world. All viruses showed the amino acid sequence 112GRQGRL117 in C-terminus extension of the F2 protein, and showed Leucine at the N-terminus extension of the F1 protein. Those sequences allowed us to determine that these viruses belonged to a type of lentogenic virus. Phylogenetic analysis showed that most of the Newcastle virus isolated in this region of Ecuador, were closely related to strains of genotype II C...
CEDAMAZ, 2019
Resumen-En varias regiones del sur del Ecuador diferentes especies de plantas son usadas para tra... more Resumen-En varias regiones del sur del Ecuador diferentes especies de plantas son usadas para tratar una variedad de dolencias. Tres extractos metanólicos de plantas usadas en la medicina tradicional en la provincia de Loja fueron investigadas con el objetivo de evaluar su posible potencial mutagénico y antimutagénico usando el ensayo de retromutación en Salmonella typhimurium (test de Ames) con las cepas bacterianas TA1535, TA100, TA1538, TA98 (ensayo de mutagenicidad), TA100 y TA98 (ensayo de antimutagenicidad) en ausencia de activación metabólica (-S9). Los extractos metanólicos de las hojas de Baccharis latifolia y Callisia repens dieron positivo para el ensayo de mutagenicidad. Ludwigia peruviana mostró propiedades antimutagénicas, contra al menos uno de los mutágenos ensayados. Estos resultados contribuyen a obtener valiosos datos sobre el uso seguro de las plantas medicinales y sus posibles efectos quimiopreventivos. Teniendo en cuenta las excelentes actividades antimutagénicas extraídas de Ludwigia peruviana estos extractos podrían ser buenas fuentes de agentes quimiopreventivos. Sin embargo, Baccharis latifolia y Callisia repens mostraron actividad mutagénica lo que sugiere precaución en su uso. La actividad mutagénica y antimutagénica de estas especies indican que son recursos valiosos para ser conservados y considerados para futuras investigaciones. Palabras clave-Extractos metanólicos; Plantas medicinales; Test de Ames Abstract-In various regions of the South of the Ecuador different plant species are used to treat a variety of ailments. Methanol extracts from plants used in traditional medicine in the province of Loja, were investigated with the objective of evaluating their possible potential mutagenic and antimutagenic using retromutation test in Salmonella typhimurium (Ames Test) with bacterial strains TA1535, TA1538, TA98 and TA100 (mutagenicity test), TA100 and TA98 (antimutagenicity test) in the absence of metabolic activation (-S9). Methanol extracts from the leaves of Baccharis latifolia and Callisia repens were positive for mutagenicity testing. Ludwigia peruviana was found to be strongly antimutagenic againts at least one of the mutagens tested. These results contribute to valuable data on the safe use of medicinal plants and their potential chemopreventive effects. Considering the excellent antimutagenic activities extracted from Ludwigia peruviana these extracts are good candidate source of chemopreventive agent. However, Baccharis latifolia and Callisia repens showed mutagenic activity, suggesting caution in their use. The mutagenic and antimutagenic activity of these species indicates that they are valuable resources that should be conserved and considered for future research. INTRODUCCIÓN E n la última década, el uso de plantas medicinales ha aumentado sustancialmente, ya sea como agentes em-pleados en medicina tradicional y/o como material de origen para la producción de suplementos dietéticos, tanto en cul-turas occidentales como asiáticas (Sponchiado et al., 2016). Datos de contacto: Willan Muñoz-Chamba, wamunoz24@gmail.com Se sabe que el uso de plantas medicinales tradicionales pre-sentes en la dieta tiene una gran importancia farmacológi-ca, atribuida a la presencia de diversos componentes fitoquí-micos. Estos compuestos químicos presentes en las plantas transmiten sus efectos biológicos ya sea actuando exclusi-va o sinérgicamente, a través de uno o más mecanismos di-ferentes (Khan et al., 2018). El uso de plantas medicinales en la medicina popular se basa en el conocimiento empírico acumulado durante siglos por grupos étnicos (Espanha et al., 2014). Desde la antigüedad, los hombres han utilizado las plantas medicinales en el tratamiento de diferentes enferme-35