Caracterización molecular y filogenética de cepas del virus de la enfermedad de Newcastle, aislado de zonas rurales del sur del Ecuador (original) (raw)
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2012
The aim of the present study was the molecular characterization of Newcastle disease virus (NDV) isolates from domestic poultry in different regions of Perú using reverse transcription chain reaction polymerase (RT-PCR) test and fusion protein sequencing. Clinical samples from fighting cook, backyard and commercial poultry were collected from outbreaks between 2009 to 2011 in Perú. The RT-PCR tests detected the Newcastle disease virus in whole clinical samples, the viruses were characterized as high pathogenic NDV strains in backyard and fighting cook; and low pathogenic NDV strains in backyard and commercial poultry. To assess the virulence of NDV isolated was amplified and sequenced the fusion protein cleavage site and was analyzed phylogenetically in order to determine the pathotype. The virulent viruses showed the sequence 113 RQKRF 117 and phenylalanine at residue 117; which is characteristic of velogenic virulent strains, whereas low virulence strains showed basic amino acid at position 113-116 and leucine at residue 117; which is characteristic of lentogenic NDV strains. The virulent NDV strains belong to genotype VII and they are closely related with virulent NDV from Peru and Malasya. The lentogenic NDV strains are grouped into genotypes I and II, both of them are very related with United States (U.S.) NDV strains.
REDVET, 2009
Análisis molecular de una cepa de virus de Newcastle de origen vacunal aislada a partir de un hisopado cloacal de aves sanas en Costa Rica http://www.veterinaria.org/revistas/redvet/n111109/111105.pdf 1 REDVET Rev. electrón. vet. http://www.veterinaria.org/revistas/redvet -http://revista.veterinaria.org Vol. 10, Nº 11, Noviembre/2009 -http://www.veterinaria.org/revistas/redvet/n111109.html Análisis molecular de una cepa de virus de Newcastle de origen vacunal aislada a partir de un hisopado cloacal de aves sanas en Costa Rica -Molecular analysis of an isolated Newcastle disease virus strain obtained from cloacal swabs in healthy poultry farm in Costa Rica
2018
The objective of the study was to develop a molecular platform for the quantification of Newcastle disease virus (NDV) from a culture system in embryonated SPF eggs. First, four pairs of primers were evaluated that amplify different regions of the NDV viral genome that code for: nucleocapsid protein (NP), protein matrix (M), fusion protein (F) and RNA-dependent RNA polymerase (L) to select the most conserved one from which a molecular platform based on reverse transcription was developed - conventional polymerase chain reaction (RT-PCRc) in two steps for the detection of NDV. Subsequently, it was taken to reverse transcription - real-time polymerase chain reaction (RT-qPCR) for the quantification of NDV produced from a system of embryonated eggs. Through these techniques, it was determined that the primers for the M gene were adequate according to the optimization criteria for the development of both methods. Sensitivity tests showed that the RT-qPCR (116 genomic copies/μl) was 10 t...
Revista de Investigaciones Veterinarias del Perú, 2014
Sera from 255 apparently nonnal birds pertaining to 16 species ofthe Psittacifonnes order, at Parque de las Leyendas Zoo in Lima were tested for the presence ofNewcastle Disease antibodies. The hemagglutination-inhibition test was used for detecting antibodies against Paramyxovirus serotype 1. Ali ofthe birds tested negative, suggesting that they have not been exposed to the Newcastle Disease virus and <lid not comprise a Paramyxovirus-1 reservoir at the moment that this study was done.
2013
Un profundo agradecimiento a las personas que nos ayudaron para que este trabajo de tesis se realice y termine de la mejor manera. En especial a todos quienes conforman la Facultad de Medicina Veterinaria y Zootecnia. Un agradecimiento especial a Al Doctor Gustavo Salgado que nos guió en su totalidad para elaborar esta investigación. Agradecemos infinitamente a los Doctores Bolívar Ricaurte, Polibio Villacís, Ana Cevallos y Marco Cisneros que con su criterio y ayuda han colaborado en la elaboración de este trabajo. De igual manera agradecemos a todos los propietarios de los criaderos, como al laboratorio Agroavilab. No podemos dejar de agradecer a nuestros familiares y compañeros que compartieron con nosotros en esta etapa de la vida que culmina hoy, para iniciar una nueva.
2019
El Parvovirus canino (CPV) es uno de los patogenos de mayor importancia en caninos jovenes, causando severos cuadros de gastroenteritis hemorragicas, con altas tasas de morbilidad y mortalidad. A nivel mundial se han descrito diferentes subtipos geneticos de CPV (2, 2a, 2b y 2c) no obstante en Mexico los estudios al respecto son escasos. El objetivo de este estudio fue caracterizar el subtipo de CPV presente en caninos de Monterrey y su area metropolitana. Para ello se analizaron 104 muestras (heces y muestras de tejidos) de caninos con problemas gastroentericos mediante PCR y PCR anidado basado en la amplificacion de un segmento de 1779 pb del gen de la proteina VP2. Se encontro que el 99.0% (103/104) de las muestras analizadas resultaron positivas para el gen VP2 del CPV. La diferenciacion del subtipo de CPV fue llevada a cabo mediante analisis de RFLP a partir de 93 muestras positivas. El subtipo CPV2c fue detectado en el 82.8% (77/93) de los casos mientras el 1.0% (1/93) mostro ...