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Papers by nathalie Van Bruaene

Research paper thumbnail of Low-dose xenogeneic mesenchymal stem cells target canine osteoarthritis through systemic immunomodulation and homing

Arthritis Research & Therapy

Background As current therapies for canine osteoarthritis (OA) provide mainly symptomatic improve... more Background As current therapies for canine osteoarthritis (OA) provide mainly symptomatic improvement and fail to address the complex pathology of the disease, mesenchymal stem cells (MSCs) offer a promising biological approach to address both aspects of OA through their immunomodulatory properties. Methods This study aimed to investigate the safety and efficacy of xenogeneic MSCs in dogs with OA at different dose levels after intravenous injection. OA was surgically induced in the right stifle joint. Thirty-two male and female dogs were divided into three treatment groups and a control group. Regular general physical examinations; lameness, joint, radiographic, and animal caretaker assessments; pressure plate analyses; and blood analyses were performed over 42 days. At study end, joint tissues were evaluated regarding gross pathology, histopathology, and immunohistochemistry. In a follow-up study, the biodistribution of intravenously injected 99mTc-labeled equine peripheral blood-d...

Research paper thumbnail of The spatial localization of 45S rRNA genes in relation to the descent of the cells in the root cortex and epidermis of Arabidopsis thaliana seedlings Belgian Society for microscopy, Beerse, 29 november 1997

Research paper thumbnail of Computer assisted track analysis of nuclear migration in living root hairs

Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen), 2001

Research paper thumbnail of Chondrogenic Priming at Reduced Cell Density Enhances Cartilage Adhesion of Equine Allogeneic MSCs - a Loading Sensitive Phenomenon in an Organ Culture Study with 180 Explants

Cellular Physiology and Biochemistry, 2015

Research paper thumbnail of Four-dimensional imaging and computer-assisted track analysis of nuclear migration in root hairs of Arabidopsis thaliana

Journal of Microscopy

A forward reliability market is presented. The market coordinates new entry through the forward p... more A forward reliability market is presented. The market coordinates new entry through the forward procurement of reliability optionsdphysical capacity bundled with a financial option to supply energy above a strike price. The market assures adequate generating resources and prices capacity from the bids of competitive new entry in an annual auction. Efficient performance incentives are maintained from a load-following obligation to supply energy above the strike price. The capacity payment fully hedges load from high spot prices, and reduces supplier risk as well. Market power is reduced in the spot market, since suppliers enter the spot market with a nearly balanced position in times of scarcity. Market power in the reliability market is addressed by not allowing existing supply to impact the capacity price. The approach, which has been adopted in New England and Colombia, is readily adapted to either a thermal system or a hydro system.

Research paper thumbnail of Equivalence of Saliva RT-qPCR Testing to Nasalthroat/Nasopharyngeal Swab Testing in the General Practitioner's Setting to Detect SARS-CoV-2

Study design: Saliva has been proposed as valid alternative for nasopharyngeal swab for RT-qPCR d... more Study design: Saliva has been proposed as valid alternative for nasopharyngeal swab for RT-qPCR detection of SARS-CoV-2. The sensitivity is generally equivalent, and it comes with much less discomfort for the patient. While there is an overall good performance in the literature for adults, there is much less information on the use of saliva in children or in the general practitioner's setting. Methods: We tested a novel commercially available saliva collection kit with a virus inactivating and

Research paper thumbnail of Root hair development in Arabidopsis thaliana: a confocal microscopy study

Root hair formation in Arabidopsis provides a model to study the pattern formation and cell fate ... more Root hair formation in Arabidopsis provides a model to study the pattern formation and cell fate specification in plants. The epidermal cell layer of the Arabidopsis root comprises hair-bearing (trichoblast) and hairless (atrichoblast) cells in files along the main axis. Epidermal cell fate is correlated with cell position, with root hair cells located over radial walls between cortical cells, and with hairless cells located directly over cortical cells. The regulation of such a position related differentiation is unknown. The fate of the epidermal cells is partly dependent on the cell lineage; however, intercellular interactions, e.g. between cortical cells, potentially also play a role in root hair morphogenesis.

Research paper thumbnail of Developmental biology of the root hairs and the structural adaptations of the cell structure in Arabidopsis thaliana

Research paper thumbnail of Imaging nucleus migration in living root hairs of Arabidopis thaliana

Research paper thumbnail of Non destructive analysis of the wax layer of apple (Malus domestica Borkh.) by means of confocal laser scanning microscopy

Non destructive analysis of the wax layer of apple (Malus domestica Borkh.) by means of confocal ... more Non destructive analysis of the wax layer of apple (Malus domestica Borkh.) by means of confocal laser scanning microscopy

Research paper thumbnail of Equivalence of saliva RT-qPCR testing to nasal-throat/nasopharyngeal swab testing in the general practitioner's setting to detect SARS-CoV-2

Study design: Saliva has been proposed as valid alternative for nasopharyngeal swab for RT-qPCR d... more Study design: Saliva has been proposed as valid alternative for nasopharyngeal swab for RT-qPCR detection of SARS-CoV-2. The sensitivity is generally equivalent, and it comes with much less discomfort for the patient. While there is an overall good performance in the literature for adults, there is much less information on the use of saliva in children or in the general practitioner's setting. Methods: We tested a novel commercially available saliva collection kit with a virus inactivating and RNA stabilizing buffer (InActiv Blue®) in matched saliva and swab samples from 245 individuals, including 216 children, collected by general practitioners. Results: Blind RT-qPCR testing of the saliva samples confirmed all 23 positives identified by swab testing (100% concordance), irrespective of age, presence of symptoms, or high-risk status. One child's saliva sample was found low positive while negative on the nasopharyngeal swab, resulting in an overall relative sensitivity of RT-...

Research paper thumbnail of Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology

Veterinary Research Communications, 2016

Research paper thumbnail of Chondrogenic Priming at Reduced Cell Density Enhances Cartilage Adhesion of Equine Allogeneic MSCs - a Loading Sensitive Phenomenon in an Organ Culture Study with 180 Explants

Cellular Physiology and Biochemistry, 2015

Research paper thumbnail of Non destructive analysis of the wax layer of apple ( Malus domestica Borkh.) by means of confocal laser scanning microscopy

Research paper thumbnail of Reorganization and in Vivo Dynamics of Microtubules during Arabidopsis Root Hair Development

Research paper thumbnail of Four-dimensional imaging and computer-assisted track analysis of nuclear migration in root hairs of Arabidopsis thaliana

Journal of Microscopy, 2003

Nuclear migration is a fundamental mechanism necessary for the proper growth and development of m... more Nuclear migration is a fundamental mechanism necessary for the proper growth and development of many eukaryotic organisms. In this study root hairs of Arabidopsis thaliana were used as a research model to gain insight into the dynamics of nuclear migration. Root hairs are long tubular outgrowths of epidermal cells and are responsible for the uptake of water and nutrients. During the development of root hairs, the nucleus migrates into the hair after the bulge is formed. The position of the nucleus relative to the tip plays an essential role in the growth process. However, what is happening to the nucleus in full-grown root hairs is still unclear. To study nuclear dynamics in living root hair cells, stably transformed plants with the fusion proteins Histone2B-YFP and NLS-GFP-GUS were used. Four-dimensional confocal laser scanning microscopy made it possible to monitor the exact position of the nucleus in different root hairs. To analyse the sequential positions of the nuclei in the root hairs, a new computer-assisted method was developed. After track analysis a number of parameters could be extracted from the movies, such as the average speed, the amplitude, direction factor and the range of movement in the root hairs. Our results show that nuclei do not reach a final position in full-grown root hairs and this sustained movement seems to be more similar in root hairs lying close to each other. Moreover, with this methodology it could be quantitatively demonstrated that the integrity of actin is necessary for nuclear movement.

Research paper thumbnail of Computer assisted track analysis of nuclear migration in living root hairs

Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen), 2001

Research paper thumbnail of Low-dose xenogeneic mesenchymal stem cells target canine osteoarthritis through systemic immunomodulation and homing

Arthritis Research & Therapy

Background As current therapies for canine osteoarthritis (OA) provide mainly symptomatic improve... more Background As current therapies for canine osteoarthritis (OA) provide mainly symptomatic improvement and fail to address the complex pathology of the disease, mesenchymal stem cells (MSCs) offer a promising biological approach to address both aspects of OA through their immunomodulatory properties. Methods This study aimed to investigate the safety and efficacy of xenogeneic MSCs in dogs with OA at different dose levels after intravenous injection. OA was surgically induced in the right stifle joint. Thirty-two male and female dogs were divided into three treatment groups and a control group. Regular general physical examinations; lameness, joint, radiographic, and animal caretaker assessments; pressure plate analyses; and blood analyses were performed over 42 days. At study end, joint tissues were evaluated regarding gross pathology, histopathology, and immunohistochemistry. In a follow-up study, the biodistribution of intravenously injected 99mTc-labeled equine peripheral blood-d...

Research paper thumbnail of The spatial localization of 45S rRNA genes in relation to the descent of the cells in the root cortex and epidermis of Arabidopsis thaliana seedlings Belgian Society for microscopy, Beerse, 29 november 1997

Research paper thumbnail of Computer assisted track analysis of nuclear migration in living root hairs

Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen), 2001

Research paper thumbnail of Chondrogenic Priming at Reduced Cell Density Enhances Cartilage Adhesion of Equine Allogeneic MSCs - a Loading Sensitive Phenomenon in an Organ Culture Study with 180 Explants

Cellular Physiology and Biochemistry, 2015

Research paper thumbnail of Four-dimensional imaging and computer-assisted track analysis of nuclear migration in root hairs of Arabidopsis thaliana

Journal of Microscopy

A forward reliability market is presented. The market coordinates new entry through the forward p... more A forward reliability market is presented. The market coordinates new entry through the forward procurement of reliability optionsdphysical capacity bundled with a financial option to supply energy above a strike price. The market assures adequate generating resources and prices capacity from the bids of competitive new entry in an annual auction. Efficient performance incentives are maintained from a load-following obligation to supply energy above the strike price. The capacity payment fully hedges load from high spot prices, and reduces supplier risk as well. Market power is reduced in the spot market, since suppliers enter the spot market with a nearly balanced position in times of scarcity. Market power in the reliability market is addressed by not allowing existing supply to impact the capacity price. The approach, which has been adopted in New England and Colombia, is readily adapted to either a thermal system or a hydro system.

Research paper thumbnail of Equivalence of Saliva RT-qPCR Testing to Nasalthroat/Nasopharyngeal Swab Testing in the General Practitioner's Setting to Detect SARS-CoV-2

Study design: Saliva has been proposed as valid alternative for nasopharyngeal swab for RT-qPCR d... more Study design: Saliva has been proposed as valid alternative for nasopharyngeal swab for RT-qPCR detection of SARS-CoV-2. The sensitivity is generally equivalent, and it comes with much less discomfort for the patient. While there is an overall good performance in the literature for adults, there is much less information on the use of saliva in children or in the general practitioner's setting. Methods: We tested a novel commercially available saliva collection kit with a virus inactivating and

Research paper thumbnail of Root hair development in Arabidopsis thaliana: a confocal microscopy study

Root hair formation in Arabidopsis provides a model to study the pattern formation and cell fate ... more Root hair formation in Arabidopsis provides a model to study the pattern formation and cell fate specification in plants. The epidermal cell layer of the Arabidopsis root comprises hair-bearing (trichoblast) and hairless (atrichoblast) cells in files along the main axis. Epidermal cell fate is correlated with cell position, with root hair cells located over radial walls between cortical cells, and with hairless cells located directly over cortical cells. The regulation of such a position related differentiation is unknown. The fate of the epidermal cells is partly dependent on the cell lineage; however, intercellular interactions, e.g. between cortical cells, potentially also play a role in root hair morphogenesis.

Research paper thumbnail of Developmental biology of the root hairs and the structural adaptations of the cell structure in Arabidopsis thaliana

Research paper thumbnail of Imaging nucleus migration in living root hairs of Arabidopis thaliana

Research paper thumbnail of Non destructive analysis of the wax layer of apple (Malus domestica Borkh.) by means of confocal laser scanning microscopy

Non destructive analysis of the wax layer of apple (Malus domestica Borkh.) by means of confocal ... more Non destructive analysis of the wax layer of apple (Malus domestica Borkh.) by means of confocal laser scanning microscopy

Research paper thumbnail of Equivalence of saliva RT-qPCR testing to nasal-throat/nasopharyngeal swab testing in the general practitioner's setting to detect SARS-CoV-2

Study design: Saliva has been proposed as valid alternative for nasopharyngeal swab for RT-qPCR d... more Study design: Saliva has been proposed as valid alternative for nasopharyngeal swab for RT-qPCR detection of SARS-CoV-2. The sensitivity is generally equivalent, and it comes with much less discomfort for the patient. While there is an overall good performance in the literature for adults, there is much less information on the use of saliva in children or in the general practitioner's setting. Methods: We tested a novel commercially available saliva collection kit with a virus inactivating and RNA stabilizing buffer (InActiv Blue®) in matched saliva and swab samples from 245 individuals, including 216 children, collected by general practitioners. Results: Blind RT-qPCR testing of the saliva samples confirmed all 23 positives identified by swab testing (100% concordance), irrespective of age, presence of symptoms, or high-risk status. One child's saliva sample was found low positive while negative on the nasopharyngeal swab, resulting in an overall relative sensitivity of RT-...

Research paper thumbnail of Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology

Veterinary Research Communications, 2016

Research paper thumbnail of Chondrogenic Priming at Reduced Cell Density Enhances Cartilage Adhesion of Equine Allogeneic MSCs - a Loading Sensitive Phenomenon in an Organ Culture Study with 180 Explants

Cellular Physiology and Biochemistry, 2015

Research paper thumbnail of Non destructive analysis of the wax layer of apple ( Malus domestica Borkh.) by means of confocal laser scanning microscopy

Research paper thumbnail of Reorganization and in Vivo Dynamics of Microtubules during Arabidopsis Root Hair Development

Research paper thumbnail of Four-dimensional imaging and computer-assisted track analysis of nuclear migration in root hairs of Arabidopsis thaliana

Journal of Microscopy, 2003

Nuclear migration is a fundamental mechanism necessary for the proper growth and development of m... more Nuclear migration is a fundamental mechanism necessary for the proper growth and development of many eukaryotic organisms. In this study root hairs of Arabidopsis thaliana were used as a research model to gain insight into the dynamics of nuclear migration. Root hairs are long tubular outgrowths of epidermal cells and are responsible for the uptake of water and nutrients. During the development of root hairs, the nucleus migrates into the hair after the bulge is formed. The position of the nucleus relative to the tip plays an essential role in the growth process. However, what is happening to the nucleus in full-grown root hairs is still unclear. To study nuclear dynamics in living root hair cells, stably transformed plants with the fusion proteins Histone2B-YFP and NLS-GFP-GUS were used. Four-dimensional confocal laser scanning microscopy made it possible to monitor the exact position of the nucleus in different root hairs. To analyse the sequential positions of the nuclei in the root hairs, a new computer-assisted method was developed. After track analysis a number of parameters could be extracted from the movies, such as the average speed, the amplitude, direction factor and the range of movement in the root hairs. Our results show that nuclei do not reach a final position in full-grown root hairs and this sustained movement seems to be more similar in root hairs lying close to each other. Moreover, with this methodology it could be quantitatively demonstrated that the integrity of actin is necessary for nuclear movement.

Research paper thumbnail of Computer assisted track analysis of nuclear migration in living root hairs

Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen), 2001