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Papers by pramila tripathi
Herbicides impact was assessed against phytopathogenic fungi Alternaria alternata using different... more Herbicides impact was assessed against phytopathogenic fungi Alternaria alternata using different concentrations on the colony growth, spores structure, spores germination and elongation of germ tube. At the 5000 ppm concentration growth of A. alternata was completely restricted but some growth was found in fluchloralin and benthiocarb treated samples at the same concentration. At low concentration (10 ppm) stimulation in growth was occurred in 2,4-D treated Petri plates. Herbicides were found to promote abnormalities in the spores structures at higher concentration. Spores of A. alternata growing on untreated PDA had mean length 27.5 um width 9.5 um; number of horizontal septa 5.0 and vertical septa were 3.0 and 2.0 respectively. At 5000 ppm concentration in 2,4-D treated medium sporulation was prohibited and colour of colony became white. Herbicides also changed the spore structure. In 2,4-D treated cultures mean length of spore decreased by 3.27 um and mean width increased by 1.80 um. Number of horizontal septa also decreased but number of vertical septa was not affected. In case of benthiocarb, spore length decreased considerably while no marked change in width was found. In fluchloralin mean length of spore was 17.12 um and mean width 12.65um. In benthiocarb and fluchloralin treated culture the mean number of horizontal septa were 1.0 and no vertical septa was observed. Percentage germination of spores and growth of germ tube was also affected by the herbicides and inhibitory effect was corresponding to the concentration of herbicides.
International Journal of Advances in Agricultural Science and Technology, 2018
Phytopathogenic fungi Botryodiplodia theobromae causes diseases such as dieback, blights, and roo... more Phytopathogenic fungi Botryodiplodia theobromae causes diseases such as dieback, blights, and root rot in a variety of different hosts in tropical and subtropical regions and it include guava, coconut, papaya and grapevine plants. It also cause stem end rot disease in mango fruits. A number of angiospermic taxa were evaluated for their antifungal activity. Among the selected plants essential oil of L. cubeba was found effective in controlling the growth of Botryodiplodia theobromae. The minimum inhibitory concentration (MIC) of the essential oil was recorded 100ppm. The oil was found to withstand high inoculums density. Plant extract were prepared in water, acetone, ethyl alcohol, petroleum ether, ethyl acetate and methanol. Plant extracts of Allamanda cathartica, Lawsonia inermis, Prunus persica and L. cubeba showed inhibitory effect on the B. theobromae. However, Leaf extract of L cubeba in all the solvent namely, water, acetone, ethyl alcohol, petroleum ether, ethyl acetate and m...
Sustainable crop disease management using natural products, 2015
This chapter reviews the recent literature on the exploitation of essential oils and plant extrac... more This chapter reviews the recent literature on the exploitation of essential oils and plant extracts for the management of plant fungal diseases of foliage, roots and seeds, postharvest and stored product commodities and other classes of pathogens, including bacteria, viral pathogens and plant parasitic nematodes.
International Journal of Current Microbiology and Applied Sciences, 2016
is a climacteric and susceptible fruit to fungal decays in postharvest stage. After harvest, frui... more is a climacteric and susceptible fruit to fungal decays in postharvest stage. After harvest, fruit rot diseases cause a severe loss of kiwifruit during cold storage, transportation, marketing, and in retail stores (Koh et al., 2003). Many fungi are associated with post harvest fruit rots of kiwifruit (Pennycook, 1985; Hawthorne et al., 1982). Botrytis gray mold rot caused by Botrytis cinerea is the most important and can directly invade the fruit or enter through wounds. Other fungal pathogens Phomopsis mali, Botryosphaeria dothidea and Diaporthe actinidiae have also been reported to cause post harvest fruit rots of kiwifruit (Koh et al., 2003). Post harvest application of dicarboximide fungicides immediately after harvest has been shown to
Annals of Plant Protection Sciences, 2021
The present study comprises pharmacological trials with the essential oils of Ocimum sanctum, Pru... more The present study comprises pharmacological trials with the essential oils of Ocimum sanctum, Prunus persica and Zingiber officinale, which have been found to exhibit a fungitoxic effect on plant pathogenic fungi. Analysis of the blood and serum of albino rats fed with an oil-supplemented diet for sixty days did not reveal significant variations in the levels of hemoglobin, total blood glucose, protein, cholesterol, urea, serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase and alkaline phosphatase nor in differential leucocyte counts, in comparison to rats fed with a normal rat diet. These essential oils thus can be recommended as safe antifungal agents because the oils did not show any adverse alteration on different pharmacological parameters that have been observed in the present piece of work.
A total of 52 fungal species were isolated from forest foil. Phoma leveillei was recorded through... more A total of 52 fungal species were isolated from forest foil. Phoma leveillei was recorded throughout the study period. Fungal species Acremonium butyric, Aspergillus niger, Aurobasidium pullulans, Cladorrhinum foecundissimum, Cunningmella elegans, Geotrichum sp., Humicola fuscoatra, Humicola grisea, Paecilomyces farinosus, Torula herbarum were dominant and found commonly during the study period.
Study was carried out for evaluation of antifungal activity of essential oil and extract of plant... more Study was carried out for evaluation of antifungal activity of essential oil and extract of plants for control of stem end rot of mango. To investigate antifungal activity leaves of 20 angiospermic taxa were extracted separately in water, acetone, ethyl acetate, ethyl alcohol, methanol and petroleum ether and screened against Botryodiplodia theobromae that cause stem end rot of mango fruits . Plant Adenocalymma alliaceum leaf extract in water, acetone, ethyl alcohol, petroleum ether, ethyl acetate and methanol was found to exhibit strongest antifungal activity. The essential oil of A. alliaceum was also found fungi toxic against other fruit rotting fungi. The volatile fraction of the leaves of A. alliaceum was isolated in the form of essential oil and was standardized through physicochemical and fungitoxic properties. The minimum inhibitory concentration (MIC) of the oil was found to be 100ppm. The oil was thermostable in nature and had a long shelf life of 2 years. The oil was foun...
Essential oils of 24 angiospermic taxa were screened for their antifungal activity against fruit ... more Essential oils of 24 angiospermic taxa were screened for their antifungal activity against fruit rotting fungus Colletotrichum musae causing anthracnose of banana. The essential oil of three plants viz. C. zeylanicum, A. indica and Mentha arvensis showed 100 % activity against the test pathogen. The essential oil of C. zeylanicum oil was selected for further investigation. The minimum inhibitory concentration of C. zeylanicum oil was found to be 100ppm. The nature of toxicity of oil was fingistatic at its MIC but turned cidal at hypertoxic concentration of 200ppm.The fungitoxicity of the oil was thermostabal up to 80 ̊C.The shelf life of oil was found to be 24 months. The C. zeylanicum oil was found to exhibit a broad fungitoxic spectrum by inhibiting the mycelia growth of 10 common fruit rotting fungi at its MIC. During in vivo trial the C. zeylanicum oil treated banana fruits showed enhancement of storage life up to 4 days. Therefore, the essential oil of C. zeylanicum could be re...
Journal of Advances in Biology & Biotechnology, 2016
Indian Phytopathology, 2001
ABSTRACT ashukla21.tripod.com
ABSTRACT ashukla21.tripod.com
Management of fungal plant pathogens
World Journal of Microbiology and Biotechnology, 2007
During screening of twenty six essential oils against Botrytis cinerea, the essential oils of the... more During screening of twenty six essential oils against Botrytis cinerea, the essential oils of the ten plants viz. Chenopodium ambrosioides, Eucalyptus citriodora, Eupatorium cannabinum, Lawsonia inermis, Ocimum canum, O. gratissimum, O. sanctum, Prunus persica, Zingiber cassumunar and Z. officinale were found to exhibit absolute fungitoxic activity (100% growth inhibition). The essential oils of O. sanctum, P. persica and Z. officinale were selected for further investigation because these oils showed lower Minimum Inhibitory Concentration (MIC) as compared to the other fungitoxic oils. The selected oils were subsequently standardized through physico-chemical and fungitoxic properties. The MIC values of O. sanctum, P. persica and Z. officinale were found to be 200, 100 and 100 ppm (mg/l) respectively. The oils showed fungistatic nature at their respective MIC. The oils were thermostable, and exhibited a wide range of fungitoxicity against 15 other post-harvest fungal pathogens. The oils had the potency to withstand high inoculum density. The antifungal potency of oils was found to be greater in comparison to some prevalent synthetic fungicides. Practical applicability of the essential oils was observed in control of grey mould of grapes caused by B. cinerea during storage. The O. sanctum-and P. persica-oil-treated grapes showed enhancement of storage life up to 5 and 4 days respectively. The storage life of Z. officinale-oil-treated grapes was found to be enhanced up to 6 days. The oils did not exhibit any phytotoxic effect on the fruit peel. Therefore, the oils could be recommended as a potential source of ecofriendly botanical fungicide, after long term and wide ranging trials.
Postharvest Biology and Technology, 2004
Chemical fungicides provide the primary means for controlling postharvest fungal decay of fruit a... more Chemical fungicides provide the primary means for controlling postharvest fungal decay of fruit and vegetables. Continuous use of fungicides has faced two major obstacles-increasing public concern regarding contamination of perishables with fungicidal residues, and proliferation of resistance in the pathogen populations. The ultimate aim of recent research in this area has been the development and evaluation of various alternative control strategies to reduce dependency on synthetic fungicides. Several non-chemical treatments have been proposed for fungal decay control. Although these approaches have been shown to reduce postharvest rots of fruit and vegetables, each has limitations that can affect their commercial applicability. When used as stand-alone treatments, none of the non-chemical control methods has been clearly shown to offer a consistently economic level of disease control that warrants acceptance as an alternative to synthetic fungicides. Recently, the exploitation of natural products to control decay and prolong storage life of perishables has received more and more attention. Biologically active natural products have the potential to replace synthetic fungicides. This review deals with exploitation of some natural products such as flavour compounds, acetic acid, jasmonates, glucosinolates, propolis, fusapyrone and deoxyfusapyrone, chitosan, essential oils and plant extracts for the management of fungal rotting of fruit and vegetables, thereby prolonging shelf life.
Fresh Produce, 2007
Considerable amounts of fruits and vegetables are lost to spoilage after harvest. This loss can r... more Considerable amounts of fruits and vegetables are lost to spoilage after harvest. This loss can range from 10-50% depending on the commodity and country. Presently, synthetic chemicals are the primary means of controlling post harvest diseases of fruits and vegetables. Public concern over food safety, however, enunciated interest to find out the effective alternatives to chemical pesticides to control post harvest diseases of perishables. The ultimate aim of recent research in this area has been the development and evaluation of various alternative control strategies to reduce dependency on synthetic fungicides. Currently several promising biological approaches that include the application of microbial antagonists (fungi, bacteria, yeasts), the natural plant based antimicrobial substances (volatile aromatic compounds, acetic acid, jasmonates, glucosinolates, essential oils, plant extracts and propolis), the antimicrobial substances from soil (fusapyrone and deoxyfusapyrone) and the natural animal-based antimicrobial substances like chitosan have been advanced to curb the menaces of post harvest diseases in perishables. Compounds that activate host plant defense responses potentially offer socioenvironmentally sound alternative methods for disease control. Combination of the above complementary techniques could well lead to effective control of post harvest diseases. The techniques and practice of using all these non-conventional alternatives is still in its infancy as compared to chemical treatments but the results and progress in this area during the past decade has been remarkable.
asianjtm.com
... Pramila Tripathi a*, NK Dubeyb , AK Shuklac a. Department of Botany, DAV, PG College, Kanpur ... more ... Pramila Tripathi a*, NK Dubeyb , AK Shuklac a. Department of Botany, DAV, PG College, Kanpur 208001, India b. Department of Botany, Banaras ... of Mentha, Ocimum and Zingiber oil, respectively, was kept in a plastic container (volume 1000 mL) containing 500 gm of animal ...
Herbicides impact was assessed against phytopathogenic fungi Alternaria alternata using different... more Herbicides impact was assessed against phytopathogenic fungi Alternaria alternata using different concentrations on the colony growth, spores structure, spores germination and elongation of germ tube. At the 5000 ppm concentration growth of A. alternata was completely restricted but some growth was found in fluchloralin and benthiocarb treated samples at the same concentration. At low concentration (10 ppm) stimulation in growth was occurred in 2,4-D treated Petri plates. Herbicides were found to promote abnormalities in the spores structures at higher concentration. Spores of A. alternata growing on untreated PDA had mean length 27.5 um width 9.5 um; number of horizontal septa 5.0 and vertical septa were 3.0 and 2.0 respectively. At 5000 ppm concentration in 2,4-D treated medium sporulation was prohibited and colour of colony became white. Herbicides also changed the spore structure. In 2,4-D treated cultures mean length of spore decreased by 3.27 um and mean width increased by 1.80 um. Number of horizontal septa also decreased but number of vertical septa was not affected. In case of benthiocarb, spore length decreased considerably while no marked change in width was found. In fluchloralin mean length of spore was 17.12 um and mean width 12.65um. In benthiocarb and fluchloralin treated culture the mean number of horizontal septa were 1.0 and no vertical septa was observed. Percentage germination of spores and growth of germ tube was also affected by the herbicides and inhibitory effect was corresponding to the concentration of herbicides.
International Journal of Advances in Agricultural Science and Technology, 2018
Phytopathogenic fungi Botryodiplodia theobromae causes diseases such as dieback, blights, and roo... more Phytopathogenic fungi Botryodiplodia theobromae causes diseases such as dieback, blights, and root rot in a variety of different hosts in tropical and subtropical regions and it include guava, coconut, papaya and grapevine plants. It also cause stem end rot disease in mango fruits. A number of angiospermic taxa were evaluated for their antifungal activity. Among the selected plants essential oil of L. cubeba was found effective in controlling the growth of Botryodiplodia theobromae. The minimum inhibitory concentration (MIC) of the essential oil was recorded 100ppm. The oil was found to withstand high inoculums density. Plant extract were prepared in water, acetone, ethyl alcohol, petroleum ether, ethyl acetate and methanol. Plant extracts of Allamanda cathartica, Lawsonia inermis, Prunus persica and L. cubeba showed inhibitory effect on the B. theobromae. However, Leaf extract of L cubeba in all the solvent namely, water, acetone, ethyl alcohol, petroleum ether, ethyl acetate and m...
Sustainable crop disease management using natural products, 2015
This chapter reviews the recent literature on the exploitation of essential oils and plant extrac... more This chapter reviews the recent literature on the exploitation of essential oils and plant extracts for the management of plant fungal diseases of foliage, roots and seeds, postharvest and stored product commodities and other classes of pathogens, including bacteria, viral pathogens and plant parasitic nematodes.
International Journal of Current Microbiology and Applied Sciences, 2016
is a climacteric and susceptible fruit to fungal decays in postharvest stage. After harvest, frui... more is a climacteric and susceptible fruit to fungal decays in postharvest stage. After harvest, fruit rot diseases cause a severe loss of kiwifruit during cold storage, transportation, marketing, and in retail stores (Koh et al., 2003). Many fungi are associated with post harvest fruit rots of kiwifruit (Pennycook, 1985; Hawthorne et al., 1982). Botrytis gray mold rot caused by Botrytis cinerea is the most important and can directly invade the fruit or enter through wounds. Other fungal pathogens Phomopsis mali, Botryosphaeria dothidea and Diaporthe actinidiae have also been reported to cause post harvest fruit rots of kiwifruit (Koh et al., 2003). Post harvest application of dicarboximide fungicides immediately after harvest has been shown to
Annals of Plant Protection Sciences, 2021
The present study comprises pharmacological trials with the essential oils of Ocimum sanctum, Pru... more The present study comprises pharmacological trials with the essential oils of Ocimum sanctum, Prunus persica and Zingiber officinale, which have been found to exhibit a fungitoxic effect on plant pathogenic fungi. Analysis of the blood and serum of albino rats fed with an oil-supplemented diet for sixty days did not reveal significant variations in the levels of hemoglobin, total blood glucose, protein, cholesterol, urea, serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase and alkaline phosphatase nor in differential leucocyte counts, in comparison to rats fed with a normal rat diet. These essential oils thus can be recommended as safe antifungal agents because the oils did not show any adverse alteration on different pharmacological parameters that have been observed in the present piece of work.
A total of 52 fungal species were isolated from forest foil. Phoma leveillei was recorded through... more A total of 52 fungal species were isolated from forest foil. Phoma leveillei was recorded throughout the study period. Fungal species Acremonium butyric, Aspergillus niger, Aurobasidium pullulans, Cladorrhinum foecundissimum, Cunningmella elegans, Geotrichum sp., Humicola fuscoatra, Humicola grisea, Paecilomyces farinosus, Torula herbarum were dominant and found commonly during the study period.
Study was carried out for evaluation of antifungal activity of essential oil and extract of plant... more Study was carried out for evaluation of antifungal activity of essential oil and extract of plants for control of stem end rot of mango. To investigate antifungal activity leaves of 20 angiospermic taxa were extracted separately in water, acetone, ethyl acetate, ethyl alcohol, methanol and petroleum ether and screened against Botryodiplodia theobromae that cause stem end rot of mango fruits . Plant Adenocalymma alliaceum leaf extract in water, acetone, ethyl alcohol, petroleum ether, ethyl acetate and methanol was found to exhibit strongest antifungal activity. The essential oil of A. alliaceum was also found fungi toxic against other fruit rotting fungi. The volatile fraction of the leaves of A. alliaceum was isolated in the form of essential oil and was standardized through physicochemical and fungitoxic properties. The minimum inhibitory concentration (MIC) of the oil was found to be 100ppm. The oil was thermostable in nature and had a long shelf life of 2 years. The oil was foun...
Essential oils of 24 angiospermic taxa were screened for their antifungal activity against fruit ... more Essential oils of 24 angiospermic taxa were screened for their antifungal activity against fruit rotting fungus Colletotrichum musae causing anthracnose of banana. The essential oil of three plants viz. C. zeylanicum, A. indica and Mentha arvensis showed 100 % activity against the test pathogen. The essential oil of C. zeylanicum oil was selected for further investigation. The minimum inhibitory concentration of C. zeylanicum oil was found to be 100ppm. The nature of toxicity of oil was fingistatic at its MIC but turned cidal at hypertoxic concentration of 200ppm.The fungitoxicity of the oil was thermostabal up to 80 ̊C.The shelf life of oil was found to be 24 months. The C. zeylanicum oil was found to exhibit a broad fungitoxic spectrum by inhibiting the mycelia growth of 10 common fruit rotting fungi at its MIC. During in vivo trial the C. zeylanicum oil treated banana fruits showed enhancement of storage life up to 4 days. Therefore, the essential oil of C. zeylanicum could be re...
Journal of Advances in Biology & Biotechnology, 2016
Indian Phytopathology, 2001
ABSTRACT ashukla21.tripod.com
ABSTRACT ashukla21.tripod.com
Management of fungal plant pathogens
World Journal of Microbiology and Biotechnology, 2007
During screening of twenty six essential oils against Botrytis cinerea, the essential oils of the... more During screening of twenty six essential oils against Botrytis cinerea, the essential oils of the ten plants viz. Chenopodium ambrosioides, Eucalyptus citriodora, Eupatorium cannabinum, Lawsonia inermis, Ocimum canum, O. gratissimum, O. sanctum, Prunus persica, Zingiber cassumunar and Z. officinale were found to exhibit absolute fungitoxic activity (100% growth inhibition). The essential oils of O. sanctum, P. persica and Z. officinale were selected for further investigation because these oils showed lower Minimum Inhibitory Concentration (MIC) as compared to the other fungitoxic oils. The selected oils were subsequently standardized through physico-chemical and fungitoxic properties. The MIC values of O. sanctum, P. persica and Z. officinale were found to be 200, 100 and 100 ppm (mg/l) respectively. The oils showed fungistatic nature at their respective MIC. The oils were thermostable, and exhibited a wide range of fungitoxicity against 15 other post-harvest fungal pathogens. The oils had the potency to withstand high inoculum density. The antifungal potency of oils was found to be greater in comparison to some prevalent synthetic fungicides. Practical applicability of the essential oils was observed in control of grey mould of grapes caused by B. cinerea during storage. The O. sanctum-and P. persica-oil-treated grapes showed enhancement of storage life up to 5 and 4 days respectively. The storage life of Z. officinale-oil-treated grapes was found to be enhanced up to 6 days. The oils did not exhibit any phytotoxic effect on the fruit peel. Therefore, the oils could be recommended as a potential source of ecofriendly botanical fungicide, after long term and wide ranging trials.
Postharvest Biology and Technology, 2004
Chemical fungicides provide the primary means for controlling postharvest fungal decay of fruit a... more Chemical fungicides provide the primary means for controlling postharvest fungal decay of fruit and vegetables. Continuous use of fungicides has faced two major obstacles-increasing public concern regarding contamination of perishables with fungicidal residues, and proliferation of resistance in the pathogen populations. The ultimate aim of recent research in this area has been the development and evaluation of various alternative control strategies to reduce dependency on synthetic fungicides. Several non-chemical treatments have been proposed for fungal decay control. Although these approaches have been shown to reduce postharvest rots of fruit and vegetables, each has limitations that can affect their commercial applicability. When used as stand-alone treatments, none of the non-chemical control methods has been clearly shown to offer a consistently economic level of disease control that warrants acceptance as an alternative to synthetic fungicides. Recently, the exploitation of natural products to control decay and prolong storage life of perishables has received more and more attention. Biologically active natural products have the potential to replace synthetic fungicides. This review deals with exploitation of some natural products such as flavour compounds, acetic acid, jasmonates, glucosinolates, propolis, fusapyrone and deoxyfusapyrone, chitosan, essential oils and plant extracts for the management of fungal rotting of fruit and vegetables, thereby prolonging shelf life.
Fresh Produce, 2007
Considerable amounts of fruits and vegetables are lost to spoilage after harvest. This loss can r... more Considerable amounts of fruits and vegetables are lost to spoilage after harvest. This loss can range from 10-50% depending on the commodity and country. Presently, synthetic chemicals are the primary means of controlling post harvest diseases of fruits and vegetables. Public concern over food safety, however, enunciated interest to find out the effective alternatives to chemical pesticides to control post harvest diseases of perishables. The ultimate aim of recent research in this area has been the development and evaluation of various alternative control strategies to reduce dependency on synthetic fungicides. Currently several promising biological approaches that include the application of microbial antagonists (fungi, bacteria, yeasts), the natural plant based antimicrobial substances (volatile aromatic compounds, acetic acid, jasmonates, glucosinolates, essential oils, plant extracts and propolis), the antimicrobial substances from soil (fusapyrone and deoxyfusapyrone) and the natural animal-based antimicrobial substances like chitosan have been advanced to curb the menaces of post harvest diseases in perishables. Compounds that activate host plant defense responses potentially offer socioenvironmentally sound alternative methods for disease control. Combination of the above complementary techniques could well lead to effective control of post harvest diseases. The techniques and practice of using all these non-conventional alternatives is still in its infancy as compared to chemical treatments but the results and progress in this area during the past decade has been remarkable.
asianjtm.com
... Pramila Tripathi a*, NK Dubeyb , AK Shuklac a. Department of Botany, DAV, PG College, Kanpur ... more ... Pramila Tripathi a*, NK Dubeyb , AK Shuklac a. Department of Botany, DAV, PG College, Kanpur 208001, India b. Department of Botany, Banaras ... of Mentha, Ocimum and Zingiber oil, respectively, was kept in a plastic container (volume 1000 mL) containing 500 gm of animal ...