sohaib peerzada - Academia.edu (original) (raw)
Papers by sohaib peerzada
Brazilian Journal of Pharmaceutical Sciences, 2019
Pakistan Veterinary Journal, 2018
The present study focuses on the use of n-hexane, chloroform, ethyl acetate and aqueous extracts ... more The present study focuses on the use of n-hexane, chloroform, ethyl acetate and aqueous extracts of Pteris cretica to confirm its in-vivo anti-inflammatory properties and in-vitro cytotoxic profile. The anti-inflammatory study was conducted by carrageenan-induced edema test at different doses of Pteris cretica extracts (250 and 500mg/kg body weight). At 250 mg/kg dose of chloroform, n-hexane, aqueous and ethyl acetate extracts expressed maximum anti-inflammatory activity at the 2 nd hour with 29.65, 29.06, 27.55 and 26.04% respectively by inhibiting carrageenaninduced edema. Moreover, 500 mg/kg concentration of chloroform extract of Pteris cretica expressed efficient anti-inflammatory activity with 45.3% of inhibition in edema; whereas n-hexane, aqueous and ethyl acetate extracts inhibit the edema at 35.34, 29.06 and 27.55% respectively. MTT assay was used to determine the cytotoxic potential of Pteris cretica extracts on HeLa, and BHK-21 cell lines. Chloroform and aqueous extracts expressed the cytotoxic activity with IC50 of 31.48 and 34.26 µg/mL respectively against HeLa cell lines. However, both chloroform and aqueous extracts exhibit IC50 108.50 and 55.76 µg/mL against BHK-21 cell line respectively. Chloroform extract is less cytotoxic against BHK-21 cell line as compared to aqueous extract. Ethyl acetate extract was found to be toxic for both cell lines. Based upon the above results, isolation, identification and purification of phytochemicals from Pteris cretica extracts is highly recommended as a possible extension of the current work.
Asian Pacific Journal of Tropical Biomedicine, 2021
Objective: To investigate the pharmacological potential of Argemone mexicana in treating constipa... more Objective: To investigate the pharmacological potential of Argemone mexicana in treating constipation and emesis by using in vitro and in vivo models. Methods: The spasmogenic and spasmolytic effects were evaluated on isolated rabbit jejunum fragments loaded in a tissue organ bath. The response was recorded with an isotonic transducer attached with Power Lab Data Acquisition System. The laxative and antiemetic activities were assessed in BALB-c mice and poultry chicks challenged with carbamylcholine and copper sulphate stimulated emesis, respectively. Results: The total phenolic and total flavonoids contents of the extract were (267.75 ± 5.77) mg GAE/g and (73.86 ± 6.01) mg QE/g, respectively. Argemone mexicana extract exerted spasmogenic effect on isolated rabbit jejunum segments with an EC50 value of 0.016 mg/ mL, which was blocked by atropine (0.3 μM). Argemone mexicana extract exerted spasmolytic effect in atropine treated jejunum fragments with an EC50 value of 2.185 mg/mL. Fur...
Pakistan journal of pharmaceutical sciences, 2020
A halophytic plant, Haloxylon stocksii, is used to treat various inflammatory disorders tradition... more A halophytic plant, Haloxylon stocksii, is used to treat various inflammatory disorders traditionally. The present study was carried out to investigate the phytochemical parameters, anti-inflammatory, analgesic and cytotoxic potential of the whole plant extracts of H. stocksii. The plant powder was standardized for pharmacognostic parameters. It was extracted with methanol followed by chloroform, ethyl acetate and water to prepare respective fractions. Total phenolic and flavonoid contents in the extract and fractions were estimated. The anti-inflammatory potential was determined through carrageenan-induced rat paw edema model. Centrally acting analgesic activity was assessed through the hot plate method. MTT assay was used to assess the viability of Human umbilical and human hepatocyte carcinoma cell lines upon exposure to plant extract/fractions. Chloroform fraction showed the highest phenolic while ethyl acetate exhibited a maximum flavonoids content. The plant ethyl acetate frac...
Pistacia khinjuk is one of the fifteen known species of Pistacia belonging to Anacardiaceae famil... more Pistacia khinjuk is one of the fifteen known species of Pistacia belonging to Anacardiaceae family. Keeping in view the possible therapeutic utility of this genus and the lack of literature on this plant, this study involves phytochemical investigation of P. khinjuk and its antioxidant activity. The phytochemical investigation was conducted on crude methanolic extract and its fractions namely, n-hexane, chloroform, n-butanol, ethyl acetate and aqueous. Total phenolic contents and flavonoids were also determined by phosphomolybdenum and ferric thiocyanate method in crude extract and its fractions. The results of phytochemical investigation indicated the presence of alkaloids, tannins, flavonoids, saponins, triterpenoids, cardiac glycosides, carbohydrates, proteins and sterols in the crude extract of P. khinjuk. Crude extract and its fractions exhibited remarkable antioxidant activity. This study showed that the crude extract and its fractions have potent antioxidant activity, among a...
Microscopy research and technique, Jan 23, 2018
A comprehensive account on standardization of herbal drug Nigella sativa L. (Tukhm-e-Kalonji) by ... more A comprehensive account on standardization of herbal drug Nigella sativa L. (Tukhm-e-Kalonji) by using microscopic as well as pharmacognostic parameters. In the field of herbal medicines, the main issues are quality, purity, and effectiveness, as in many cases herbal drugs are knowingly or unknowingly substituted or adulterated with similar species or varieties. In herbal market the seeds of N. sativa are commonly adultered with seeds of Allium cepa L. intentionally or accidentally due to their similar morphology. In the present study, the microscopic characterization of herbal drug was done by morphological, palynological, and anatomical features. A great diversity was found in anatomical features of two species, as irregular epidermal cells, actinocytic stomata, and nonglandular trichomes were found in N. sativa while in A. cepa epidermal cells were rectangular in shape, stomata type was paracytic, and trichomes were absent. Pharmacognostic characterization was made by fluorescenc...
EXCLI journal, 2017
Lack of data on safety of herbal medicines have endangered human health and life. The present stu... more Lack of data on safety of herbal medicines have endangered human health and life. The present study evaluated the genotoxic and mutagenic effect of Kalanchoe laciniata to access the safety and usefulness of the medicinal plant. Aqua-methanolic and n-hexane extracts of K. laciniata were evaluated for the genotoxic potential using Ames assay and cytotoxicity was evaluated using MTT assay. Ames assay was conducted using two strains of Salmonella typhimurium TA-100 and TA-102 whereas MTT assay was performed on baby hamster kidney cell line BHK-21. Aqua-methanolic extract of K.laciniata exhibited significant mutagenicity when exposed to TA-102 strain with a mutagenic index of 50.66 and 54.74 at maximum dose 150 mg/plate. The extract was also mutagenic to TA-100 strain but to a lesser extent. M.I of n-hexane extract was 12.15 and 15.51 for TA-100 and TA-102 respectively. n-hexane extract was mutagenic but little difference was observed between results of two strains. Both extracts were fo...
EXCLI journal, 2016
Viral hepatitis, an inflammatory liver disease, is caused by various genotypes of hepatitis C vir... more Viral hepatitis, an inflammatory liver disease, is caused by various genotypes of hepatitis C viruses (HCV). Hepatitis C slowly sprouts into fibrosis, which progresses to cirrhosis. Over a prolonged period of time compensated cirrhosis can advance to decompensated cirrhosis culminating in hepatic failure and death. Conventional treatment of HCV involves the administration of interferons. However, association of interferon with the adverse drug reactions led to the development of novel anti-HCV drugs given as monotherapy or in combination with the other drugs. Advances in drug delivery systems (DDS) improved the pharmacokinetic profile and stability of drugs, ameliorated tissue damages on extravasation and increased the targeting of affected sites. Liposomes and lipid based vehicles have been employed with polyethylene glycol (PEG) so as to stabilize the formulations as PEG drug complex. Sofosbuvir, a novel anti-HCV drug, is administered as monotherapy or in combination with daclatas...
EXCLI journal, 2016
Most herbal medicines utilized in complementary and alternative medicine lack safety evaluation s... more Most herbal medicines utilized in complementary and alternative medicine lack safety evaluation setting our lives under unwarranted risks. Present study comprised of genotoxic and cytotoxic appraisal of Terminalia citrina fruits which are used as a folklore medicine for treatment of various ailments. Aqueous and ethanolic extracts of T. citrina fruit extracts were evaluated for the presence of different phytochemicals. Genotoxic potential of both the extract of T. citrina was assessed through Ames reverse mutagenicity assay in Salmonella TA 100 and 102 strains. Cytotoxic potential of T. citrina was determined in baby hamster kidney cell line (BHK-21). Statistical analysis was carried out by ANOVA following post hoc test. Phytochemical analysis showed the presence of alkaloids, carbohydrates, phenolic compounds, tannins, catechins and saponins. It was revealed that both the extracts of T. citrina exhibited significant mutagenicity in tester strains. Ethanolic extract showed higher mu...
Tropical Journal of Pharmaceutical Research, 2016
Purpose: To explore the literature regarding prevalance, risk factors and the involvement of anti... more Purpose: To explore the literature regarding prevalance, risk factors and the involvement of antihypertensive drugs in erectile dysfunction (ED). Methods: Original research articles, reviews, editorials and case reports published in English language on the prevalence of sexual/erectile dysfunction in hypertensive men taking antihypertensive drugs and risk factors were identified through a search of four bibliographic databases, namely, PubMed, EMBASE, CINAHL and EBSCO Health. Results: Recent analyses suggest that hypertensive men of almost all age groups suffer from ED but it is more prevalent in elderly male patients. The involvement of β-blockers was found to be controversial. Nevertheless, some evidence had been found regarding the use of propranolol in high doses. Conclusion: The present review indicates the need for research to unravel the role of β-blockers in the manifestation of ED in hypertensive males, whom there are no contributory factors such as sedentary lifestyle, aging, stress and anxiety, etc.
Background: Plants and herbs have long been used as remedies without scientific evidences. The ob... more Background: Plants and herbs have long been used as remedies without scientific evidences. The objective of the present study was to explore the anti-inflammatory, anti-nociceptive and antipyretic potential of ethanolic and aqueous extracts of Terminalia citrina fruits in mice. Materials and Methods: Extracts of Terminalia citrina fruits were evaluated at doses of 200mg/kg, 400mg/kg and 600mg/kg in albino mice for preventive effect in inflammatory edema, peripheral pain sensation and pyrexia. Carrageenan induced paw edema method was utilized to evaluate anti-inflammatory activity. Analgesic appraisal of extracts was demonstrated using acetic acid induced writhing model of pain. Antipyretic potential was determined by brewer’s yeast induced pyrexia model. Statistical analysis was conducted by ANOVA following post hoc test. Results: Both extracts exhibited significant and dose-dependent anti-inflammatory, analgesic and antipyretic activities. The ethanolic extract was more effective i...
Cellular and Molecular Biology
Brazilian Journal of Pharmaceutical Sciences, 2019
Pakistan Veterinary Journal, 2018
The present study focuses on the use of n-hexane, chloroform, ethyl acetate and aqueous extracts ... more The present study focuses on the use of n-hexane, chloroform, ethyl acetate and aqueous extracts of Pteris cretica to confirm its in-vivo anti-inflammatory properties and in-vitro cytotoxic profile. The anti-inflammatory study was conducted by carrageenan-induced edema test at different doses of Pteris cretica extracts (250 and 500mg/kg body weight). At 250 mg/kg dose of chloroform, n-hexane, aqueous and ethyl acetate extracts expressed maximum anti-inflammatory activity at the 2 nd hour with 29.65, 29.06, 27.55 and 26.04% respectively by inhibiting carrageenaninduced edema. Moreover, 500 mg/kg concentration of chloroform extract of Pteris cretica expressed efficient anti-inflammatory activity with 45.3% of inhibition in edema; whereas n-hexane, aqueous and ethyl acetate extracts inhibit the edema at 35.34, 29.06 and 27.55% respectively. MTT assay was used to determine the cytotoxic potential of Pteris cretica extracts on HeLa, and BHK-21 cell lines. Chloroform and aqueous extracts expressed the cytotoxic activity with IC50 of 31.48 and 34.26 µg/mL respectively against HeLa cell lines. However, both chloroform and aqueous extracts exhibit IC50 108.50 and 55.76 µg/mL against BHK-21 cell line respectively. Chloroform extract is less cytotoxic against BHK-21 cell line as compared to aqueous extract. Ethyl acetate extract was found to be toxic for both cell lines. Based upon the above results, isolation, identification and purification of phytochemicals from Pteris cretica extracts is highly recommended as a possible extension of the current work.
Asian Pacific Journal of Tropical Biomedicine, 2021
Objective: To investigate the pharmacological potential of Argemone mexicana in treating constipa... more Objective: To investigate the pharmacological potential of Argemone mexicana in treating constipation and emesis by using in vitro and in vivo models. Methods: The spasmogenic and spasmolytic effects were evaluated on isolated rabbit jejunum fragments loaded in a tissue organ bath. The response was recorded with an isotonic transducer attached with Power Lab Data Acquisition System. The laxative and antiemetic activities were assessed in BALB-c mice and poultry chicks challenged with carbamylcholine and copper sulphate stimulated emesis, respectively. Results: The total phenolic and total flavonoids contents of the extract were (267.75 ± 5.77) mg GAE/g and (73.86 ± 6.01) mg QE/g, respectively. Argemone mexicana extract exerted spasmogenic effect on isolated rabbit jejunum segments with an EC50 value of 0.016 mg/ mL, which was blocked by atropine (0.3 μM). Argemone mexicana extract exerted spasmolytic effect in atropine treated jejunum fragments with an EC50 value of 2.185 mg/mL. Fur...
Pakistan journal of pharmaceutical sciences, 2020
A halophytic plant, Haloxylon stocksii, is used to treat various inflammatory disorders tradition... more A halophytic plant, Haloxylon stocksii, is used to treat various inflammatory disorders traditionally. The present study was carried out to investigate the phytochemical parameters, anti-inflammatory, analgesic and cytotoxic potential of the whole plant extracts of H. stocksii. The plant powder was standardized for pharmacognostic parameters. It was extracted with methanol followed by chloroform, ethyl acetate and water to prepare respective fractions. Total phenolic and flavonoid contents in the extract and fractions were estimated. The anti-inflammatory potential was determined through carrageenan-induced rat paw edema model. Centrally acting analgesic activity was assessed through the hot plate method. MTT assay was used to assess the viability of Human umbilical and human hepatocyte carcinoma cell lines upon exposure to plant extract/fractions. Chloroform fraction showed the highest phenolic while ethyl acetate exhibited a maximum flavonoids content. The plant ethyl acetate frac...
Pistacia khinjuk is one of the fifteen known species of Pistacia belonging to Anacardiaceae famil... more Pistacia khinjuk is one of the fifteen known species of Pistacia belonging to Anacardiaceae family. Keeping in view the possible therapeutic utility of this genus and the lack of literature on this plant, this study involves phytochemical investigation of P. khinjuk and its antioxidant activity. The phytochemical investigation was conducted on crude methanolic extract and its fractions namely, n-hexane, chloroform, n-butanol, ethyl acetate and aqueous. Total phenolic contents and flavonoids were also determined by phosphomolybdenum and ferric thiocyanate method in crude extract and its fractions. The results of phytochemical investigation indicated the presence of alkaloids, tannins, flavonoids, saponins, triterpenoids, cardiac glycosides, carbohydrates, proteins and sterols in the crude extract of P. khinjuk. Crude extract and its fractions exhibited remarkable antioxidant activity. This study showed that the crude extract and its fractions have potent antioxidant activity, among a...
Microscopy research and technique, Jan 23, 2018
A comprehensive account on standardization of herbal drug Nigella sativa L. (Tukhm-e-Kalonji) by ... more A comprehensive account on standardization of herbal drug Nigella sativa L. (Tukhm-e-Kalonji) by using microscopic as well as pharmacognostic parameters. In the field of herbal medicines, the main issues are quality, purity, and effectiveness, as in many cases herbal drugs are knowingly or unknowingly substituted or adulterated with similar species or varieties. In herbal market the seeds of N. sativa are commonly adultered with seeds of Allium cepa L. intentionally or accidentally due to their similar morphology. In the present study, the microscopic characterization of herbal drug was done by morphological, palynological, and anatomical features. A great diversity was found in anatomical features of two species, as irregular epidermal cells, actinocytic stomata, and nonglandular trichomes were found in N. sativa while in A. cepa epidermal cells were rectangular in shape, stomata type was paracytic, and trichomes were absent. Pharmacognostic characterization was made by fluorescenc...
EXCLI journal, 2017
Lack of data on safety of herbal medicines have endangered human health and life. The present stu... more Lack of data on safety of herbal medicines have endangered human health and life. The present study evaluated the genotoxic and mutagenic effect of Kalanchoe laciniata to access the safety and usefulness of the medicinal plant. Aqua-methanolic and n-hexane extracts of K. laciniata were evaluated for the genotoxic potential using Ames assay and cytotoxicity was evaluated using MTT assay. Ames assay was conducted using two strains of Salmonella typhimurium TA-100 and TA-102 whereas MTT assay was performed on baby hamster kidney cell line BHK-21. Aqua-methanolic extract of K.laciniata exhibited significant mutagenicity when exposed to TA-102 strain with a mutagenic index of 50.66 and 54.74 at maximum dose 150 mg/plate. The extract was also mutagenic to TA-100 strain but to a lesser extent. M.I of n-hexane extract was 12.15 and 15.51 for TA-100 and TA-102 respectively. n-hexane extract was mutagenic but little difference was observed between results of two strains. Both extracts were fo...
EXCLI journal, 2016
Viral hepatitis, an inflammatory liver disease, is caused by various genotypes of hepatitis C vir... more Viral hepatitis, an inflammatory liver disease, is caused by various genotypes of hepatitis C viruses (HCV). Hepatitis C slowly sprouts into fibrosis, which progresses to cirrhosis. Over a prolonged period of time compensated cirrhosis can advance to decompensated cirrhosis culminating in hepatic failure and death. Conventional treatment of HCV involves the administration of interferons. However, association of interferon with the adverse drug reactions led to the development of novel anti-HCV drugs given as monotherapy or in combination with the other drugs. Advances in drug delivery systems (DDS) improved the pharmacokinetic profile and stability of drugs, ameliorated tissue damages on extravasation and increased the targeting of affected sites. Liposomes and lipid based vehicles have been employed with polyethylene glycol (PEG) so as to stabilize the formulations as PEG drug complex. Sofosbuvir, a novel anti-HCV drug, is administered as monotherapy or in combination with daclatas...
EXCLI journal, 2016
Most herbal medicines utilized in complementary and alternative medicine lack safety evaluation s... more Most herbal medicines utilized in complementary and alternative medicine lack safety evaluation setting our lives under unwarranted risks. Present study comprised of genotoxic and cytotoxic appraisal of Terminalia citrina fruits which are used as a folklore medicine for treatment of various ailments. Aqueous and ethanolic extracts of T. citrina fruit extracts were evaluated for the presence of different phytochemicals. Genotoxic potential of both the extract of T. citrina was assessed through Ames reverse mutagenicity assay in Salmonella TA 100 and 102 strains. Cytotoxic potential of T. citrina was determined in baby hamster kidney cell line (BHK-21). Statistical analysis was carried out by ANOVA following post hoc test. Phytochemical analysis showed the presence of alkaloids, carbohydrates, phenolic compounds, tannins, catechins and saponins. It was revealed that both the extracts of T. citrina exhibited significant mutagenicity in tester strains. Ethanolic extract showed higher mu...
Tropical Journal of Pharmaceutical Research, 2016
Purpose: To explore the literature regarding prevalance, risk factors and the involvement of anti... more Purpose: To explore the literature regarding prevalance, risk factors and the involvement of antihypertensive drugs in erectile dysfunction (ED). Methods: Original research articles, reviews, editorials and case reports published in English language on the prevalence of sexual/erectile dysfunction in hypertensive men taking antihypertensive drugs and risk factors were identified through a search of four bibliographic databases, namely, PubMed, EMBASE, CINAHL and EBSCO Health. Results: Recent analyses suggest that hypertensive men of almost all age groups suffer from ED but it is more prevalent in elderly male patients. The involvement of β-blockers was found to be controversial. Nevertheless, some evidence had been found regarding the use of propranolol in high doses. Conclusion: The present review indicates the need for research to unravel the role of β-blockers in the manifestation of ED in hypertensive males, whom there are no contributory factors such as sedentary lifestyle, aging, stress and anxiety, etc.
Background: Plants and herbs have long been used as remedies without scientific evidences. The ob... more Background: Plants and herbs have long been used as remedies without scientific evidences. The objective of the present study was to explore the anti-inflammatory, anti-nociceptive and antipyretic potential of ethanolic and aqueous extracts of Terminalia citrina fruits in mice. Materials and Methods: Extracts of Terminalia citrina fruits were evaluated at doses of 200mg/kg, 400mg/kg and 600mg/kg in albino mice for preventive effect in inflammatory edema, peripheral pain sensation and pyrexia. Carrageenan induced paw edema method was utilized to evaluate anti-inflammatory activity. Analgesic appraisal of extracts was demonstrated using acetic acid induced writhing model of pain. Antipyretic potential was determined by brewer’s yeast induced pyrexia model. Statistical analysis was conducted by ANOVA following post hoc test. Results: Both extracts exhibited significant and dose-dependent anti-inflammatory, analgesic and antipyretic activities. The ethanolic extract was more effective i...
Cellular and Molecular Biology