srinivas hebbar - Academia.edu (original) (raw)

Uploads

Papers by srinivas hebbar

Journal of Young Pharmacists, 2021

Objectives: To formulate and investigate the role of various penetration enhancers in the transfe... more Objectives: To formulate and investigate the role of various penetration enhancers in the transfersomal gel delivery of adapalene. Methods: Transfersomes were formulated by reverse-phase evaporation method and the influence of various parameters was investigated and optimized for particle size, PDI, zeta potential, and entrapment efficiency using the Design of Experiment approach. Optimized formulation was subjected to various characterizations such as Fourier Transform Infrared Spectroscopy, turbidity measurement, and deformability study, and then transfersomes were incorporated into a suitable gel (Carbopol 934). The transfersomal gel was then characterized for gel characteristics such as pH, spreadability, viscosity, homogeneity, extrudability, etc. Appropriate essential oils used as natural permeation enhancers like eucalyptus oil, menthol, peppermint oil, and turpentine oil were included in the transfersomal gel of adapalene, and then in vitro drug release, ex vivo permeation, ...

Phytosomes are novel form of herbal formulations which contains the bioactive phytoconstituent(s)... more Phytosomes are novel form of herbal formulations which contains the bioactive phytoconstituent(s) of herb extract complexed with phospholipid to produce lipid compatible molecular complexes. Since phytoconstituents are obtained from natural resources, fewer side effects and lower phytochemical costs are added advantages for their utilization in treatment of various diseases. Unfortunately, despite the wide therapeutic potentials of poly phenolic phytoconstituents such as flavonoids, glycosides, terpenoids etc. still they suffer with poor aqueous solubility, absorption and bioavailability problems when administered orally or by topical applications. The effectiveness of any herbal product is dependent upon delivering an effective level of active compounds. The phytosome technology meets this challenge by markedly enhancing the solubility, absorption and bioavailability of the phytomedicines for better drug delivery and clinical action. It exhibit better pharmacokinetic and pharmacody...

AAPS PharmSciTech, 2020

Adapalene-loaded transfersome gel containing vitamin C as a combination therapy for the managemen... more Adapalene-loaded transfersome gel containing vitamin C as a combination therapy for the management of acne vulgaris was developed in the present study. The transfersome was prepared by reverse-phase evaporation, and the effect of various process parameters were investigated by the Design of Experiment (DOE) approach and optimized based on the particle size (PS), polydispersity index (PDI), zeta potential (ZP), and entrapment efficiency (EE). The selected tranfersomes were further evaluated for their thermal behavior and morphology by transmission electron microscopy and turbidity measurements and incorporated into a gel with/without vitamin C. The gel was evaluated and compared with the marketed product (Adiff gel) for various physicochemical parameters, and in vivo studies in testosterone-induced rat models of acne. The prepared transfersomes had PS in the range of 280 to 400 nm, PDI values of 0.416 to 0.8, ZP of − 38 to − 20 mV, and % EE of 32 to 70%. DSC studies confirmed a positive interaction of the components in the transfersome. Surface morphology confirmed that the vesicles were spherical, unilamellar, and discrete. A relative deformability study showed higher elasticity of the transfersomes compared with Adiff aqs gel. Ascorbyl-6-palmitate in adapalene-loaded transfersome gel containing vitamin C (ADVTG) was found to have a good antioxidant free radical-scavenging activity. An in vitro drug release study showed that the sustained release of the transfersomal formulations was attributed to the flexibility of the vesicles by which penetration was increased. ADVTG was found to be promising in treating acne compared with the marketed product.

International Journal of Pharmaceutical Investigation, 2018

Delivering drugs via oral route represents the most widely and convenient route being the easiest... more Delivering drugs via oral route represents the most widely and convenient route being the easiest in administration, the most compliant for the patient, in addition to, the possibility to design many dosage forms. Moreover, it is the most economical, especially that, it is characterized by the least sterility constraints. However, the major challenge with the formulation of oral dosage forms lies with their poor oral bioavailability. Various factors such as poor aqueous solubility, lower drug permeability, Objective: The present study was aimed at formulating and evaluating a novel solid self-nano emulsifying drug delivery system (SNEDDS) to increase the solubility and bioavailability of hydrochlorothiazide (HCZ). Enhancing both solubility and bioavailability of drugs remain the cornerstone for achieving successful outcomes of delivery systems. Furthermore, employing nanotechnology-based formulations such as SNEDDS offers important advantage; the most important is the protection of the drug from enzymatic or chemical degradation. Materials and Methods: Liquid SNEDDS (L-SNEDDS) was prepared by adding a drug to oil, surfactant, and co-surfactant and heated up to at 60°C under continuous stirring. Solid SNEDDS (S-SNEDDS) was prepared by mixing L-SNEDDS with microcrystalline cellulose in 1:1 proportion. Results: The scanning electron microscopy showed that S-SNEDDS was spherical with an average particle size of 66.9 nm and 69.2 nm for both L-SNEDDS and S-SNEDDS, respectively. Ex vivo skin permeation study indicated that 100% drug was released from both the L-SNEDDS and S-SNEDDS formulation SF3 in 3 h. Analysis of variance test showed significant differences (Moderately significant P < 0.01) in the values when compared to a marketed product. Conclusion: The prepared S-SNEDDS helped in improving the solubility of the poorly soluble HCZ, which is a step forward toward bioavailability enhancement and thus increased therapeutic efficacy of the drug.

International Journal of Applied Pharmaceutics, 2019

Objective: Asiatic acid is a triterpene saponin and the main constituent of the species Centellaa... more Objective: Asiatic acid is a triterpene saponin and the main constituent of the species Centellaasiatica. The current study aimed to isolate, characterize and develop an analytical method for asiatic acid in the shorter run time with good efficiency. Methods: In this study isolation of asiatic acid was achieved by TLC and thus isolated asiatic acid was characterized by 1HNMR spectral analysis and LCMS. An isocratic RP-HPLC method was established for the assessment of asiatic acid from the methanol extract of Centellaasiatica. The chromatographic separations were achieved by RP-C18 column 250x4.6 mm (5µ Particle size) Shimadzu UFLC pump LC 20AD and mobile phase composed of 0.1% orthophosphoric acid: acetonitrile (50:50). The analysis of column effluents was achieved using a PDA detector (Photo Diode Array) at 210 nm, and the flow rate was recorded 1 ml/min. Results: Soxhlet extraction process followed by fractional extraction using different solvents was performed. Methnol extract wa...

AAPS PharmSciTech, 2018

The current study was aimed to develop an amphiphilic drug-lipid nanocomplex of rutin:egg phospha... more The current study was aimed to develop an amphiphilic drug-lipid nanocomplex of rutin:egg phosphatidylcholine (EPC) to enhance its poor absorption and bioavailability, and investigated the impact of the complex on hepatoprotective and antioxidant activity. Rutin nano-complexes were prepared by solvent evaporation, salting out and lyophilisation methods and compared for the complex formation. For the selected lyophilisation method, principal solvent DMSO, co-solvent (t-butyl alcohol) and rutin:EPC ratios (1:1, 1:2 and 1:3) were selected after optimisation. The properties of the nanocomplexes such as complexation, thermal behaviour, surface morphology, molecular crystallinity, particle size, zeta potential, drug content, solubility, in vitro stability study, in vitro drug release, in vitro and in vivo antioxidant study, in vivo hepatoprotective activity and oral bioavailability/pharmacokinetic studies were investigated. Rutin nano-complexes were developed successfully via the lyophilisation method and found to be in nanometric range. Rutin nano-complexes significantly improved the solubility and in vitro drug release, and kinetic studies confirmed the diffusion-controlled release of the drug from the formulation. The nano-complex showed better antioxidant activity in vitro and exhibited well in vitro stability in different pH media. The in vivo study showed better hepatoprotective activity of the formulation compared to pure rutin at the same dose levels with improved oral bioavailability. Carbon tetrachloride (CCl 4)-treated animals (group II) failed to restore the normal levels of serum hepatic marker enzymes and liver antioxidant enzyme compared to the nano-complex-treated animals. The results obtained from solubility, hepatoprotective activity and oral bioavailability studies proved the better efficacy of the nano-complex compared to the pure drug.

Indian Journal of Pharmaceutical Education and Research, 2018

Objective: The aim of this observational research study was to bring up the issues related to Com... more Objective: The aim of this observational research study was to bring up the issues related to Communication skill (CS) and Good documentation practice (GDP) in one of the pharmacy college in India and its impact on the students in their overall personality development and job perspectives. These skills are like an accelerator which is required to facilitate the smooth sailing of the business. Methods: The new study model was developed and employed to conduct this observational research study for final year B. Pharm and M. Pharm students which is called as Classroom Record-Daily laboratory Record-Human resource Record (CR-DR-HR) model. Classroom data collected through continuous learning activity from everyday class assessment, Daily laboratory data collected through the practical records and laboratory assessments and Human resource (HR) feedback was collected, analysed and then conveyed by HR department to the investigators. A course end evaluation was conducted through the administration of a questionnaire to all participants. Results and Discussion: The result clearly suggested that the students who are effectively communicating and efficiently marshalling the documents have been enjoying their positions better than those who did not acquire this quality in college level. The findings of the study suggested that the curricula should adopt CS and GDP as the main line practice where students should be allowed to learn these practices right from the beginning of the semesters. Conclusion: Teaching and learning curriculum programs should emphasis on to create the proper environment to enhance communication and interpersonal skills that help students to develop personal effectiveness which ultimately empowers them with the feeling of employability

International Journal of Applied Pharmaceutics, 2017

Objective: The aim of the present study was to prepare hydrogel matrix tablets for controlled rel... more Objective: The aim of the present study was to prepare hydrogel matrix tablets for controlled release of an anti-asthma drug (Montelukast sodium) by modifying the applications of chitosan by crosslinking it with the different cross linking agent.Methods: The hydrogels were prepared by crosslinking chitosan using three different crosslinking agents namely, anhydrous dextrose (DXT), sodium tripolyphosphate (TPP) and glutaraldehyde (GL). Formulations were prepared by direct compression method and pre and post compression parameters were evaluated.Results: FTIR (Fourier transform infrared spectroscopy) studies of tablet formulation indicated that there is no drug-excipient interaction in the prepared formulations. The matrix tablets were capable of releasing the drug for 11 h depending upon the formulation variables. The tablets prepared by plain chitosan discharged the drug quickly, while those prepared by using GL crosslinked-hydrogel released the drug more slowly in a controlled mann...

F1000Research

Background: Asiatic acid (AA) is a naturally occurring triterpenoid derivative of Centella asiati... more Background: Asiatic acid (AA) is a naturally occurring triterpenoid derivative of Centella asiatica (CA) with neuroprotective effect. The study aimed to design an ideal oral drug delivery system to treat Alzheimer's disease (AD) and develop chitosan-embedded liposomes comprising an extract of CA (CLCAE) and compare them with the chitosan-coated liposomes of asiatic acid (CLAA) for oral delivery to treat the initial phases of AD. Methods: The solvent evaporation technique was used to develop CLCAE and CLAA, optimised with the experiment's design, and was further evaluated. Results: Nuclear magnetic resonance (NMR) studies confirmed coating with chitosan. Transmission electron microscopy (TEM) and atomic force microscopy (AFM) indicated the successful formation of CLCAE and CLAA. Differential scanning colorimetry (DSC) confirmed the drug-phospholipid complex. Furthermore, the rate of in vitro release of CLCAE and CLAA was found to be 69.43±0.3 % and 85.3±0.3 %, respectively, ...