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Papers by tarso rudiana

Pharmaceutical and Biomedical Sciences Journal (Online)/Pharmaceutical and Biomedical Sciences Journal, Jan 23, 2024

Jurnal farmasi sains dan praktis, May 14, 2023

Degenerative diseases can be caused by free radicals, whereas antioxidants can overcome free radi... more Degenerative diseases can be caused by free radicals, whereas antioxidants can overcome free radicals. Ambarella leaves (Spondias dulcis Parkinson) and soursop leaves (Annona muricata Linn.) are potential sources of suitable antioxidant compounds. This study aims to determine the antioxidant activity and the relationship between antioxidants and total phenolics from the combination of S. dulcis and A. muricata leaf extracts. S. dulcis leaves, and A. muricata leaves were macerated with 96% ethanol and then formulated with a combination of extracts (1:1, 2:1, and 1:2). Each was tested for its antioxidant activity by the DPPH method. The ethanol extract of S. dulcis leaves had an antioxidant activity value of 36.72±4.01 ppm with a total phenol of 76.84±0.50 mgGAE/g extract, an ethanol extract of A. muricata leaves of 81.45±8.19 ppm with a total phenol of 104.48±7.55 mg GAE/g extract and a combination of extracts with a ratio of ethanol extract of S. dulcis leaves and ethanol extract of A. muricata leaves (2:1) of 29.98 ± 0.37 ppm with a total phenol of 36.99 ± 0.98 mgGAE/g extract. The results showed that the phenolic content of the ethanol extract of A. muricata leaves was more significant than the combination (2:1), which had the most excellent antioxidant activity among the other comparisons. Total phenolic is inversely related to antioxidant activity. Antioxidant activity is not only played by phenolic compounds.

Jurnal Pengabdian Pada Masyarakat

Swamedikasi (pengobatan sendiri) meningkat dikalangan masyarakat, tidak terkecuali masyarakat di ... more Swamedikasi (pengobatan sendiri) meningkat dikalangan masyarakat, tidak terkecuali masyarakat di Desa Kutakarang, Cibitung Kabupaten Pandeglang. Hal ini perlu diimbangi dengan pengetahuan cara mengelola obat dengan baik dan benar agarr obat dapat memberikan efek terapeutik yang diharapkan dan menghindari kesalahan dalam penggunaan dan pengelolaan obat. DAGUSIBU adalah dapat, guna, simpan dan buang obat dengan benar merupakan salah satu upaya peningkatan penggunaan obat bagi masyarakat yang diselenggarakan melalui kegiatan pelayanan kesehatan oleh tenaga kefarmasian. Pelayanan kesehatan yang dapat diberikan tenaga kefarmasian kepada masyarakat yakni kegiatan pemberian informasi tentang penggunaan dan penyimpanan sediaan farmasi dan alat kesehatan. Tujuan pengabdian ini adalah peningkatan pengetahuan masyarakat dalam memahami cara mendapatkan, menggunakan, menyimpan dan membuang obat, serta memahami cara mengkonsumsi obat dengan baik dan benar. Metode yang digunakan adalah presentasi ...

THE 5TH INTERNATIONAL CONFERENCE ON BIOSCIENCE AND BIOTECHNOLOGY

Jurnal kimia Valensi, May 31, 2018

Telah dilakukan penelitian untuk mengetahui struktur senyawa fenolik yang memiliki aktivitas anti... more Telah dilakukan penelitian untuk mengetahui struktur senyawa fenolik yang memiliki aktivitas antioksidan dari ekstrak etil asetat biji honje (E. elatior). Isolasi senyawa fenolik dilakukan dengan metode maserasi, fraksinasi dengan kromatografi kolom gravitasi (KKG), dan kromatografi lapis tipis (KLT). Uji aktivitas antioksidan menggunakan metode DPPH (2,2-diphenyl-1-picrylhydrazyl) dan penentuan struktur senyawa menggunakan spektroskopi UV-Vis, FTIR, NMR, dan MS. Isolat yang diperoleh berupa gum kuning sebanyak 18 mg dari 3 kg sampel kering. Hasil uji aktivitas antioksidan menunjukkan isolat dari ekstrak etil asetat memiliki aktivitas yang sangat kuat dengan IC 50 1,32 µg/mL. Hasil analisis dengan spektroskopi UV-Vis, FTIR, NMR, dan MS menunjukkan isolat sesuai dengan rumus molekul C 7 H 6 O 4 yang dikenal dengan asam protokatekuat (asam 3,4dihidroksi benzoat).

Jurnal Pendidikan Kimia, Dec 30, 2022

Antioxidant activity and identification of flavonoid compounds in Patat leaves (Phrynium capitatu... more Antioxidant activity and identification of flavonoid compounds in Patat leaves (Phrynium capitatum) ethyl acetate extract.

Jurnal kimia terapan Indonesia, Jun 22, 2017

Active compounds with antioxidant activity were isolated from ethyl acetate extract of namnam ste... more Active compounds with antioxidant activity were isolated from ethyl acetate extract of namnam stem (C. cauliflora L.) that had undergone maceration and fractionation by gravity column chromatography. The compounds were later identified by by using UV-Vis Spectrophotometry, FTIR, LCMS and 1 H-NMR. Ethyl acetate extract of namnam stem showed considerably high antioxidant activity (IC 50 value 4.68 ± 0.035 ppm). The results of analysis by UV-Vis and FTIR showed carbonyl group conjugated with an aromatic ring DW EDQG , max 330.22 nm), chromophore group of alkene (C=C) at band II max QP DQG IXQFWLRQDO JURXSV VXFK DV 2í+ FP-1), C=O (1729.23 cm-1), C=C (1652.64 and 1611.99 cm-1 &í2 DQG &í+ ortho (738.23 cm-1). LCMS (m/z 270.9246) and 1 H-NMR data showed seven proton signals on the aromatic ring at carbon position C-3 at / H 6.86 ppm (1H, s), C-6 at / H 5.95 ppm (1H, d, J=1.95 Hz), C-8 at / H 6.25 ppm (1H, d, J= 1.95 Hz), C-¶ and C-¶ at / H 7.03 ppm (2H, d, J=7.87 Hz), C-¶ and C-¶ at / H 6.87 ppm (2H, d, J= 7.87 Hz) so that the structure was identified as a flavonoid which was 4 ', 5,7-trihydroxy-flavones or known as apigenin. The isolated apigenin had very strong antioxidant activity, as shown by IC 50 value of 5.18 ± 0.014 ppm.

Alchemy: Journal of Chemistry, Oct 30, 2021

Indonesia has the largest and most biodiverse coral reef in the world. Colt coral has not been st... more Indonesia has the largest and most biodiverse coral reef in the world. Colt coral has not been studied and explored especially endophytic fungi associated with the coral. Endophytic fungi are highly potential for the production of antioxidant and anticancer compounds. This research aimed to study the antioxidant and cytotoxic activities of fermentation extract from endophytic fungi from colt coral. Filtrate and mycelium extracts were obtained from static and shake fermentations of isolate SKF 15. Antioxidant and cytotoxic assays were conducted by free radical scavenger 1,1-diphenyl-2-picrylhydrazyl (DPPH) and AlamarBlue methods, respectively. The result showed that FD extract provided the highest antioxidant activity with inhibition of 49.36% at 200 ppm of DPPH. Variation of fermentation time (3-21 days) demonstrated the highest activity with inhibition of 66.97% for antioxidant assay (7 days) and 81.13% for cytotoxic assay (3 days). FTIR analysis presented the existence of hydroxyl groups O-H (3452.58 cm-1), C=C groups (1668.43 cm-1); CO hydroxyl group (1230.58 cm-1), and C-H sp 3 groups (2941.44 cm-1). Based on LC-MS analysis, FD extract has a mass of m/z 305.63, [M+H] + , predicted as dihydroquercetin (C15H24O7).

Molecules

Murraya is a plant genus within the Rutaceae family comprising over 17 species, which are widely ... more Murraya is a plant genus within the Rutaceae family comprising over 17 species, which are widely distributed in Asia, Australia, and the Pacific Islands. Furthermore, these species have been used in traditional medicine to treat fever, pain, and dysentery. Several reports have also extensively studied the leaves, seeds, stembark, and bark of Murraya from 1965 to 2023 to explore their natural product composition. Various phytochemical studies have revealed the isolation of 413 compounds recorded, comprising coumarins, terpenoids, flavonoids, and aromatics, as well as alkaloids, which constitute the largest proportion (46.9%). These isolated compounds have long been known to exhibit different bioactivities, such as cytotoxic and anti-inflammatory properties. Cytotoxic activity has been observed against HCT 116, HeLa, HepG2, and other cell lines. Previous studies have also reported the presence of antifungal, hepatoprotective, antihyperlipidemic, antidiarrheal, and antioxidant effects....

ALCHEMY:Journal of Chemistry

Gandaria (Bouea macrophylla) plants contain antioxidant compounds that can reduce free radicals. ... more Gandaria (Bouea macrophylla) plants contain antioxidant compounds that can reduce free radicals. Free radicals are one of the causes of various degenerative diseases. The purpose of this study was to determine the fraction that had the highest antioxidant activity, and to determine the secondary metabolites contained in the most active fraction of B. macrophylla seeds based on LCMS/MS analysis. Maceration of B. macrophylla seeds with ethanol solvent. Ethanol maserate was concentrated and fractionated by liquid-liquid extraction method using n-hexane, ethyl acetate and ethanol as solvents. Antioxidant activity was measured by the free radical scavenger method with 1,1-diphenyl-2-picrylhidrazil as a source of radicals and characterization of the secondary metabolites contained in the most active fraction using a Liquid Chromatography-Mass Spectrometer. B. macrophylla seed ethanol extract has a % yield of 31.75%. The ethyl acetate fraction of B. macrophylla seeds has the highest mass c...

Jurnal Sains Farmasi & Klinis

Soybean is a source of vegetable protein that is rich in nutrients and a source of producing anti... more Soybean is a source of vegetable protein that is rich in nutrients and a source of producing anticancer bioactive peptides. This study aims to determine the optimum conditions for hydrolysis of soybean protein with papain enzyme and their cytotoxicity against the MCF-7 cells. Soybean protein was isolated using an acid precipitation technique. Then, the protein isolate was hydrolyzed using papain enzyme with variations of papain concentration 0,5; 1; and 5% (v/v) and variations of incubation time 0, 1, 2, 3, and 4 hours at 50 °C. The hydrolysates were tested for their degree of hydrolysis (%DH), molecular weight profile using SDS-PAGE, and cytotoxicity against the MCF-7 cells through an in-vitro assay. The most active hydrolysate was fractionated using Sephadex G-15 and characterized by the molecular weight by LCMS/MS. The result showed that the optimum condition for hydrolysis was 1% (v/v) of enzyme concentration and 3 hours of incubation time with a %DH value of 3.01%. Based on the...

Journal of Physics: Conference Series, 2019

Akway (Drimys beccariana, Gibbs) is a plant endemic to Papua highlands contained in Manokwari. D.... more Akway (Drimys beccariana, Gibbs) is a plant endemic to Papua highlands contained in Manokwari. D. beccariana is used for traditional malaria treatment in West Papua. The methanol extract of stem bark of D. beccariana showed antimalarial activity against Plasmodium falciparum parasites, in vitro. Methanol extract of D. beccariana is partitioned between water and n-hexane (1:9), and ethyl acetate. Furthermore, ethyl acetate extract was separated through several combination of chromatography techniques on silica gel, resulting in three known compounds (1-3). The chemical structures of compounds (1-3) were identified based on IR, UV/Vis, 1H NMR and 13C NMR, and MS data as 5,3’-dihydroxy-7,4’-dimethoxyflavone (1), 5,7-dihydroxy-4’-methoxyflavone (2), and 5-hydroxy-7,3’,4’-trimethoxyflavone (3). Compounds 1-3 showed strong antiplasmodium against 3D7 strain of P. falciparum with IC50 values of 0.002, 0.005, and 0.001 micro Molar, respectively. This result indicates that stem bark of D. bec...

Jurnal Pengabdian Pada Masyarakat

Desa wisata merupakan sebuah kawasan pedesaan yang memiliki keunikan dan karakteristik khusus unt... more Desa wisata merupakan sebuah kawasan pedesaan yang memiliki keunikan dan karakteristik khusus untuk menjadi destinasi wisata. Masyarakat disekitar desa wisata akan melakukan persiapan untuk memperbaiki kondisi desanya bersama-sama dengan aparat desa dan mempersiapkan diri menghadapi kedatangan wisatawan yang akan datang ke desa mereka. Ramea merupakan desa wisata yang dikembangkan di Kabupaten Pandeglang. Tujuan pengabdian ini adalah mengembangkan desa wisata Ramea melalui pengembangan ekonomi kreatif masyarakat dengan menggali potensi desa untuk menjadi desa wisata yang unggul. Pemberdayaan desa wisata Ramea dilakukan dengan pemberdayaan masyarakat melalui peningkatan usaha kecil mikro, pengembangan fasilitas infrastruktur penunjang wisata dan pengembangan aktivitas masyarakat dalam pengembangan pengelolaan desa wisata berkesanambungan. Proses dilakukan secara bertahap dan berkelanjutan. Hasil yang dicapai pemberdayaan yang dilakukan diikuti oleh sebagian masyarakat menjadikan h...

Jurnal Pendidikan Kimia

Flavonoids are the biggest compound from the phenolic group that has the function of antioxidants... more Flavonoids are the biggest compound from the phenolic group that has the function of antioxidants. One of the plants with flavonoids is patat (Phrynium capitatum), which is usually used as food wrapping material. This study aims to quantify total flavonoid content, antioxidant activity and identify flavonoids from patat leaves ethyl acetate extract. Patat leaves sample cleaned, dried, and grinded. Grinded patat leaves were macerated gradually for 2 × 24 hours with n-hexane, ethyl acetate, and methanol solvent. The extract undergoing total flavonoids test guided with AlCl3 and CH3COONa. Extract with the biggest flavonoids content proceed to antioxidant activity test by SOD (superoxide dismutase) method and analyzed for the flavonoid structure by UHPLC MS/MS (ORBITRAP HRMS). The biggest total flavonoids are ethyl acetate extract with 8.678 mg QE/g; then methanol extract with 5.296 mg QE/g; and n-hexane extract was not tested because of negative results in the qualitative test. The ant...

Jurnal Farmasi Udayana

Herba ketumpang air (Peperomia pellucida L) mengandung senyawa bioaktif seperti alkaloid, saponin... more Herba ketumpang air (Peperomia pellucida L) mengandung senyawa bioaktif seperti alkaloid, saponin, flavonoid, dan steroid yang diduga berkhasiat sebagai antihiperkolesterolemia. Tujuan penelitian ini adalah untuk mengetahui efektivitas ekstrak P. pellucida sebagai antihiperkolesterolemia secara in vitro dan mengkarakterisasi fraksi aktifnya. Ekstraksi menggunakan metode ultrasonikasi dengan pelarut organik secara bertingkat yaitu n-heksana, etil asetat, dan metanol. Fraksi n-heksana, etil asetat, dan metanol diuji aktivitas penurunan kolesterol secara in vitro dengan metode fotometri, kolesterol direaksikan dengan pereaksi Lieberman-Burchard kemudian dianalisis dengan menggunakan spektrofotometer UV-Vis. Fraksi yang memiliki aktivitas terbaik dikarakterisasi dengan menggunakan Liquid Chromatography Mass Spectrometer (LCMS). Fraksi etil asetat memiliki aktivitas penurunan kadar kolesterol paling baik dengan nilai nilai EC50 sebesar 196,43 ppm. Berdasarkan uji fitokimia fraksi aktif e...

Chimica et Natura Acta, 2021

Antioksidan merupakan senyawa yang dapat meredam radikal bebas. Salah satu tanaman yang berpotens... more Antioksidan merupakan senyawa yang dapat meredam radikal bebas. Salah satu tanaman yang berpotensi sebagai sumber antioksidan alami adalah Alkesa (Pouteria campechiana). Tanaman P. campechiana mengandung senyawa fenolik, flavonoid, tanin katekat, monoterpen, dan seskuiterpen. Senyawa flavonoid dan fenolik dilaporkan memiliki aktivitas antioksidan. Tujuan penelitian ini adalah menguji aktivitas antioksidan dari ekstrak kulit batang P. campechiana dan profiling senyawa metabolit sekunder pada ekstrak teraktifnya. Kulit batang P. campechiana diekstraksi secara bertahap dengan menggunakan pelarut organik dengan berbagai tingkat kepolaran diantaranya n-heksana, etil asetat, dan metanol. Masing-masing ekstrak dilakukan pengujian aktivitas antioksidan dengan metode DPPH. Ekstrak metanol memiliki aktivitas antioksidan terbaik dengan nilai IC 50 sebesar 73,89 ppm, diikuti oleh ekstrak etil asetat dan n-heksana dengan nilai IC 50 masing-masing sebesar 84,03 dan 339,10 ppm. Bedasarkan analisis LCMS/MS Ekstrak metanol mengandung senyawa kafein dan acaciin.

Telah dilakukan penelitian analisis kandungan fenolik dan aktivitas antioksidan dari ekstrak etan... more Telah dilakukan penelitian analisis kandungan fenolik dan aktivitas antioksidan dari ekstrak etanol daun salam(Syzygium polyanthum (Wight) Walp.) dan daun kelor (Moringa oleifera Lam.). Penelitian ini bertujuan untukmengetahui aktivitas antioksidan dan total fenolik pada ekstrak dan kombinasi ekstrak etanol daun salam dandaun kelor. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol. Ekstrak etanol daunsalam dan daun kelor serta kombinasi dengan formula 1:1; 2:1; dan 1:2 diuji aktivitas penghambatan radikalbebas DPPH (1,1-Difenil-2- Pikrilhidrazil). Ekstrak dan formula terbaik sebagai antioksidan dianalisis totalfenolik. Hasil penelitian menunjukkan bahwa ekstrak etanol daun salam memiliki aktivitas antioksidan dengannilai IC50 sebesar 31,14 ppm dengan total fenolik sebesar 111,89 mg GAE/g. Ekstrak etanol daun kelormemiliki aktivitas antioksidan dengan nilai IC50 sebesar 71,27 ppm dengan total fenolik sebesar 17,12 mgGAE/g. Kombinasi ekstrak etanol daun salam dan ...

FITOFARMAKA: Jurnal Ilmiah Farmasi

Alpha Hydroxy Acid (AHA) adalah asam organik yang terdiri dari 2 (dua) rantai karbon atau lebih y... more Alpha Hydroxy Acid (AHA) adalah asam organik yang terdiri dari 2 (dua) rantai karbon atau lebih yang semakin panjang rantai karbonnya akan semakin besar berat molekulnya. AHA adalah kelas senyawa kimia yang sering digunakan dalam kosmetik dan dermatologi. Beberapa buah dilaporkan mengandung senyawa AHA yaitu, asam sitrat dalam buah jeruk dan nanas, asam glikolat dalam tebu, asam laktat dalam tomat, asam malat dalam apel dan anggur, dan asam tartrat dalam anggur. Penelitian ini dilakukan untuk menetapkan kadar AHA yang terkandung dalam buah ceremai (Phyllanthus acidus L.) dengan menggunakan metode Kromatografi Cair Kinerja Tinggi (KCKT). Sari buah P.acidus dikeringkan dengan metode pengeringan beku. Kemudian senyawa AHA dalam ekstrak kering P.acidus yaitu asam glikolat dan asam sitrat dianalisis menggunakan KCKT dengan jenis kolom Capcell PAK C18 UG120S-5mm(4,6 X 250 mm); detektor UV Vis 210 nm; kecepatan alir 0,5 ml/menit; fase gerak asam fosfat dan asetonitril. Hasil penetapan kada...

Jurnal Farmasi dan Ilmu Kefarmasian Indonesia, Apr 26, 2022

Background: Kidney stones are one of the causes of chronic and acute kidney failure symptoms. The... more Background: Kidney stones are one of the causes of chronic and acute kidney failure symptoms. The flavonoid compounds in Chromolaena odorata leave extract are thought to dissolve calcium in kidney stones. Objective: This study aims to determine the activity of C. odorata leaves extract as a dissolution agent for calcium kidney stones and to characterize the active extract with a liquid chromatograph mass spectrometry. Methods: The leaves of C. odorata were extracted by ultrasonication method using 3 solvents in stages, namely n-hexane, ethyl acetate, and methanol. The powder for kidney stones was immersed in an extract solution of n-hexane, ethyl acetate, and methanol for 5 hours at 37°C. The reaction results were analyzed for their absorbance using a UV-Vis spectrophotometer. The fraction with the best activity was analyzed for phytochemical content with various typical reagents and LCMS/MS. Results: Methanol extract of C. odorata with a concentration of 10,000 g/mL can reduce calcium in kidney stones by 19,2 µg/mL. Based on phytochemical tests and LCMS/MS analysis, the methanol extract of C. odorata leaves contains compounds of the tannins, alkaloids, flavonoids, and steroids. Chromatogram at a retention time of 7.

Pharmaceutical and Biomedical Sciences Journal (Online)/Pharmaceutical and Biomedical Sciences Journal, Jan 23, 2024

Jurnal farmasi sains dan praktis, May 14, 2023

Degenerative diseases can be caused by free radicals, whereas antioxidants can overcome free radi... more Degenerative diseases can be caused by free radicals, whereas antioxidants can overcome free radicals. Ambarella leaves (Spondias dulcis Parkinson) and soursop leaves (Annona muricata Linn.) are potential sources of suitable antioxidant compounds. This study aims to determine the antioxidant activity and the relationship between antioxidants and total phenolics from the combination of S. dulcis and A. muricata leaf extracts. S. dulcis leaves, and A. muricata leaves were macerated with 96% ethanol and then formulated with a combination of extracts (1:1, 2:1, and 1:2). Each was tested for its antioxidant activity by the DPPH method. The ethanol extract of S. dulcis leaves had an antioxidant activity value of 36.72±4.01 ppm with a total phenol of 76.84±0.50 mgGAE/g extract, an ethanol extract of A. muricata leaves of 81.45±8.19 ppm with a total phenol of 104.48±7.55 mg GAE/g extract and a combination of extracts with a ratio of ethanol extract of S. dulcis leaves and ethanol extract of A. muricata leaves (2:1) of 29.98 ± 0.37 ppm with a total phenol of 36.99 ± 0.98 mgGAE/g extract. The results showed that the phenolic content of the ethanol extract of A. muricata leaves was more significant than the combination (2:1), which had the most excellent antioxidant activity among the other comparisons. Total phenolic is inversely related to antioxidant activity. Antioxidant activity is not only played by phenolic compounds.

Jurnal Pengabdian Pada Masyarakat

Swamedikasi (pengobatan sendiri) meningkat dikalangan masyarakat, tidak terkecuali masyarakat di ... more Swamedikasi (pengobatan sendiri) meningkat dikalangan masyarakat, tidak terkecuali masyarakat di Desa Kutakarang, Cibitung Kabupaten Pandeglang. Hal ini perlu diimbangi dengan pengetahuan cara mengelola obat dengan baik dan benar agarr obat dapat memberikan efek terapeutik yang diharapkan dan menghindari kesalahan dalam penggunaan dan pengelolaan obat. DAGUSIBU adalah dapat, guna, simpan dan buang obat dengan benar merupakan salah satu upaya peningkatan penggunaan obat bagi masyarakat yang diselenggarakan melalui kegiatan pelayanan kesehatan oleh tenaga kefarmasian. Pelayanan kesehatan yang dapat diberikan tenaga kefarmasian kepada masyarakat yakni kegiatan pemberian informasi tentang penggunaan dan penyimpanan sediaan farmasi dan alat kesehatan. Tujuan pengabdian ini adalah peningkatan pengetahuan masyarakat dalam memahami cara mendapatkan, menggunakan, menyimpan dan membuang obat, serta memahami cara mengkonsumsi obat dengan baik dan benar. Metode yang digunakan adalah presentasi ...

THE 5TH INTERNATIONAL CONFERENCE ON BIOSCIENCE AND BIOTECHNOLOGY

Jurnal kimia Valensi, May 31, 2018

Telah dilakukan penelitian untuk mengetahui struktur senyawa fenolik yang memiliki aktivitas anti... more Telah dilakukan penelitian untuk mengetahui struktur senyawa fenolik yang memiliki aktivitas antioksidan dari ekstrak etil asetat biji honje (E. elatior). Isolasi senyawa fenolik dilakukan dengan metode maserasi, fraksinasi dengan kromatografi kolom gravitasi (KKG), dan kromatografi lapis tipis (KLT). Uji aktivitas antioksidan menggunakan metode DPPH (2,2-diphenyl-1-picrylhydrazyl) dan penentuan struktur senyawa menggunakan spektroskopi UV-Vis, FTIR, NMR, dan MS. Isolat yang diperoleh berupa gum kuning sebanyak 18 mg dari 3 kg sampel kering. Hasil uji aktivitas antioksidan menunjukkan isolat dari ekstrak etil asetat memiliki aktivitas yang sangat kuat dengan IC 50 1,32 µg/mL. Hasil analisis dengan spektroskopi UV-Vis, FTIR, NMR, dan MS menunjukkan isolat sesuai dengan rumus molekul C 7 H 6 O 4 yang dikenal dengan asam protokatekuat (asam 3,4dihidroksi benzoat).

Jurnal Pendidikan Kimia, Dec 30, 2022

Antioxidant activity and identification of flavonoid compounds in Patat leaves (Phrynium capitatu... more Antioxidant activity and identification of flavonoid compounds in Patat leaves (Phrynium capitatum) ethyl acetate extract.

Jurnal kimia terapan Indonesia, Jun 22, 2017

Active compounds with antioxidant activity were isolated from ethyl acetate extract of namnam ste... more Active compounds with antioxidant activity were isolated from ethyl acetate extract of namnam stem (C. cauliflora L.) that had undergone maceration and fractionation by gravity column chromatography. The compounds were later identified by by using UV-Vis Spectrophotometry, FTIR, LCMS and 1 H-NMR. Ethyl acetate extract of namnam stem showed considerably high antioxidant activity (IC 50 value 4.68 ± 0.035 ppm). The results of analysis by UV-Vis and FTIR showed carbonyl group conjugated with an aromatic ring DW EDQG , max 330.22 nm), chromophore group of alkene (C=C) at band II max QP DQG IXQFWLRQDO JURXSV VXFK DV 2í+ FP-1), C=O (1729.23 cm-1), C=C (1652.64 and 1611.99 cm-1 &í2 DQG &í+ ortho (738.23 cm-1). LCMS (m/z 270.9246) and 1 H-NMR data showed seven proton signals on the aromatic ring at carbon position C-3 at / H 6.86 ppm (1H, s), C-6 at / H 5.95 ppm (1H, d, J=1.95 Hz), C-8 at / H 6.25 ppm (1H, d, J= 1.95 Hz), C-¶ and C-¶ at / H 7.03 ppm (2H, d, J=7.87 Hz), C-¶ and C-¶ at / H 6.87 ppm (2H, d, J= 7.87 Hz) so that the structure was identified as a flavonoid which was 4 ', 5,7-trihydroxy-flavones or known as apigenin. The isolated apigenin had very strong antioxidant activity, as shown by IC 50 value of 5.18 ± 0.014 ppm.

Alchemy: Journal of Chemistry, Oct 30, 2021

Indonesia has the largest and most biodiverse coral reef in the world. Colt coral has not been st... more Indonesia has the largest and most biodiverse coral reef in the world. Colt coral has not been studied and explored especially endophytic fungi associated with the coral. Endophytic fungi are highly potential for the production of antioxidant and anticancer compounds. This research aimed to study the antioxidant and cytotoxic activities of fermentation extract from endophytic fungi from colt coral. Filtrate and mycelium extracts were obtained from static and shake fermentations of isolate SKF 15. Antioxidant and cytotoxic assays were conducted by free radical scavenger 1,1-diphenyl-2-picrylhydrazyl (DPPH) and AlamarBlue methods, respectively. The result showed that FD extract provided the highest antioxidant activity with inhibition of 49.36% at 200 ppm of DPPH. Variation of fermentation time (3-21 days) demonstrated the highest activity with inhibition of 66.97% for antioxidant assay (7 days) and 81.13% for cytotoxic assay (3 days). FTIR analysis presented the existence of hydroxyl groups O-H (3452.58 cm-1), C=C groups (1668.43 cm-1); CO hydroxyl group (1230.58 cm-1), and C-H sp 3 groups (2941.44 cm-1). Based on LC-MS analysis, FD extract has a mass of m/z 305.63, [M+H] + , predicted as dihydroquercetin (C15H24O7).

Molecules

Murraya is a plant genus within the Rutaceae family comprising over 17 species, which are widely ... more Murraya is a plant genus within the Rutaceae family comprising over 17 species, which are widely distributed in Asia, Australia, and the Pacific Islands. Furthermore, these species have been used in traditional medicine to treat fever, pain, and dysentery. Several reports have also extensively studied the leaves, seeds, stembark, and bark of Murraya from 1965 to 2023 to explore their natural product composition. Various phytochemical studies have revealed the isolation of 413 compounds recorded, comprising coumarins, terpenoids, flavonoids, and aromatics, as well as alkaloids, which constitute the largest proportion (46.9%). These isolated compounds have long been known to exhibit different bioactivities, such as cytotoxic and anti-inflammatory properties. Cytotoxic activity has been observed against HCT 116, HeLa, HepG2, and other cell lines. Previous studies have also reported the presence of antifungal, hepatoprotective, antihyperlipidemic, antidiarrheal, and antioxidant effects....

ALCHEMY:Journal of Chemistry

Gandaria (Bouea macrophylla) plants contain antioxidant compounds that can reduce free radicals. ... more Gandaria (Bouea macrophylla) plants contain antioxidant compounds that can reduce free radicals. Free radicals are one of the causes of various degenerative diseases. The purpose of this study was to determine the fraction that had the highest antioxidant activity, and to determine the secondary metabolites contained in the most active fraction of B. macrophylla seeds based on LCMS/MS analysis. Maceration of B. macrophylla seeds with ethanol solvent. Ethanol maserate was concentrated and fractionated by liquid-liquid extraction method using n-hexane, ethyl acetate and ethanol as solvents. Antioxidant activity was measured by the free radical scavenger method with 1,1-diphenyl-2-picrylhidrazil as a source of radicals and characterization of the secondary metabolites contained in the most active fraction using a Liquid Chromatography-Mass Spectrometer. B. macrophylla seed ethanol extract has a % yield of 31.75%. The ethyl acetate fraction of B. macrophylla seeds has the highest mass c...

Jurnal Sains Farmasi & Klinis

Soybean is a source of vegetable protein that is rich in nutrients and a source of producing anti... more Soybean is a source of vegetable protein that is rich in nutrients and a source of producing anticancer bioactive peptides. This study aims to determine the optimum conditions for hydrolysis of soybean protein with papain enzyme and their cytotoxicity against the MCF-7 cells. Soybean protein was isolated using an acid precipitation technique. Then, the protein isolate was hydrolyzed using papain enzyme with variations of papain concentration 0,5; 1; and 5% (v/v) and variations of incubation time 0, 1, 2, 3, and 4 hours at 50 °C. The hydrolysates were tested for their degree of hydrolysis (%DH), molecular weight profile using SDS-PAGE, and cytotoxicity against the MCF-7 cells through an in-vitro assay. The most active hydrolysate was fractionated using Sephadex G-15 and characterized by the molecular weight by LCMS/MS. The result showed that the optimum condition for hydrolysis was 1% (v/v) of enzyme concentration and 3 hours of incubation time with a %DH value of 3.01%. Based on the...

Journal of Physics: Conference Series, 2019

Akway (Drimys beccariana, Gibbs) is a plant endemic to Papua highlands contained in Manokwari. D.... more Akway (Drimys beccariana, Gibbs) is a plant endemic to Papua highlands contained in Manokwari. D. beccariana is used for traditional malaria treatment in West Papua. The methanol extract of stem bark of D. beccariana showed antimalarial activity against Plasmodium falciparum parasites, in vitro. Methanol extract of D. beccariana is partitioned between water and n-hexane (1:9), and ethyl acetate. Furthermore, ethyl acetate extract was separated through several combination of chromatography techniques on silica gel, resulting in three known compounds (1-3). The chemical structures of compounds (1-3) were identified based on IR, UV/Vis, 1H NMR and 13C NMR, and MS data as 5,3’-dihydroxy-7,4’-dimethoxyflavone (1), 5,7-dihydroxy-4’-methoxyflavone (2), and 5-hydroxy-7,3’,4’-trimethoxyflavone (3). Compounds 1-3 showed strong antiplasmodium against 3D7 strain of P. falciparum with IC50 values of 0.002, 0.005, and 0.001 micro Molar, respectively. This result indicates that stem bark of D. bec...

Jurnal Pengabdian Pada Masyarakat

Desa wisata merupakan sebuah kawasan pedesaan yang memiliki keunikan dan karakteristik khusus unt... more Desa wisata merupakan sebuah kawasan pedesaan yang memiliki keunikan dan karakteristik khusus untuk menjadi destinasi wisata. Masyarakat disekitar desa wisata akan melakukan persiapan untuk memperbaiki kondisi desanya bersama-sama dengan aparat desa dan mempersiapkan diri menghadapi kedatangan wisatawan yang akan datang ke desa mereka. Ramea merupakan desa wisata yang dikembangkan di Kabupaten Pandeglang. Tujuan pengabdian ini adalah mengembangkan desa wisata Ramea melalui pengembangan ekonomi kreatif masyarakat dengan menggali potensi desa untuk menjadi desa wisata yang unggul. Pemberdayaan desa wisata Ramea dilakukan dengan pemberdayaan masyarakat melalui peningkatan usaha kecil mikro, pengembangan fasilitas infrastruktur penunjang wisata dan pengembangan aktivitas masyarakat dalam pengembangan pengelolaan desa wisata berkesanambungan. Proses dilakukan secara bertahap dan berkelanjutan. Hasil yang dicapai pemberdayaan yang dilakukan diikuti oleh sebagian masyarakat menjadikan h...

Jurnal Pendidikan Kimia

Flavonoids are the biggest compound from the phenolic group that has the function of antioxidants... more Flavonoids are the biggest compound from the phenolic group that has the function of antioxidants. One of the plants with flavonoids is patat (Phrynium capitatum), which is usually used as food wrapping material. This study aims to quantify total flavonoid content, antioxidant activity and identify flavonoids from patat leaves ethyl acetate extract. Patat leaves sample cleaned, dried, and grinded. Grinded patat leaves were macerated gradually for 2 × 24 hours with n-hexane, ethyl acetate, and methanol solvent. The extract undergoing total flavonoids test guided with AlCl3 and CH3COONa. Extract with the biggest flavonoids content proceed to antioxidant activity test by SOD (superoxide dismutase) method and analyzed for the flavonoid structure by UHPLC MS/MS (ORBITRAP HRMS). The biggest total flavonoids are ethyl acetate extract with 8.678 mg QE/g; then methanol extract with 5.296 mg QE/g; and n-hexane extract was not tested because of negative results in the qualitative test. The ant...

Jurnal Farmasi Udayana

Herba ketumpang air (Peperomia pellucida L) mengandung senyawa bioaktif seperti alkaloid, saponin... more Herba ketumpang air (Peperomia pellucida L) mengandung senyawa bioaktif seperti alkaloid, saponin, flavonoid, dan steroid yang diduga berkhasiat sebagai antihiperkolesterolemia. Tujuan penelitian ini adalah untuk mengetahui efektivitas ekstrak P. pellucida sebagai antihiperkolesterolemia secara in vitro dan mengkarakterisasi fraksi aktifnya. Ekstraksi menggunakan metode ultrasonikasi dengan pelarut organik secara bertingkat yaitu n-heksana, etil asetat, dan metanol. Fraksi n-heksana, etil asetat, dan metanol diuji aktivitas penurunan kolesterol secara in vitro dengan metode fotometri, kolesterol direaksikan dengan pereaksi Lieberman-Burchard kemudian dianalisis dengan menggunakan spektrofotometer UV-Vis. Fraksi yang memiliki aktivitas terbaik dikarakterisasi dengan menggunakan Liquid Chromatography Mass Spectrometer (LCMS). Fraksi etil asetat memiliki aktivitas penurunan kadar kolesterol paling baik dengan nilai nilai EC50 sebesar 196,43 ppm. Berdasarkan uji fitokimia fraksi aktif e...

Chimica et Natura Acta, 2021

Antioksidan merupakan senyawa yang dapat meredam radikal bebas. Salah satu tanaman yang berpotens... more Antioksidan merupakan senyawa yang dapat meredam radikal bebas. Salah satu tanaman yang berpotensi sebagai sumber antioksidan alami adalah Alkesa (Pouteria campechiana). Tanaman P. campechiana mengandung senyawa fenolik, flavonoid, tanin katekat, monoterpen, dan seskuiterpen. Senyawa flavonoid dan fenolik dilaporkan memiliki aktivitas antioksidan. Tujuan penelitian ini adalah menguji aktivitas antioksidan dari ekstrak kulit batang P. campechiana dan profiling senyawa metabolit sekunder pada ekstrak teraktifnya. Kulit batang P. campechiana diekstraksi secara bertahap dengan menggunakan pelarut organik dengan berbagai tingkat kepolaran diantaranya n-heksana, etil asetat, dan metanol. Masing-masing ekstrak dilakukan pengujian aktivitas antioksidan dengan metode DPPH. Ekstrak metanol memiliki aktivitas antioksidan terbaik dengan nilai IC 50 sebesar 73,89 ppm, diikuti oleh ekstrak etil asetat dan n-heksana dengan nilai IC 50 masing-masing sebesar 84,03 dan 339,10 ppm. Bedasarkan analisis LCMS/MS Ekstrak metanol mengandung senyawa kafein dan acaciin.

Telah dilakukan penelitian analisis kandungan fenolik dan aktivitas antioksidan dari ekstrak etan... more Telah dilakukan penelitian analisis kandungan fenolik dan aktivitas antioksidan dari ekstrak etanol daun salam(Syzygium polyanthum (Wight) Walp.) dan daun kelor (Moringa oleifera Lam.). Penelitian ini bertujuan untukmengetahui aktivitas antioksidan dan total fenolik pada ekstrak dan kombinasi ekstrak etanol daun salam dandaun kelor. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol. Ekstrak etanol daunsalam dan daun kelor serta kombinasi dengan formula 1:1; 2:1; dan 1:2 diuji aktivitas penghambatan radikalbebas DPPH (1,1-Difenil-2- Pikrilhidrazil). Ekstrak dan formula terbaik sebagai antioksidan dianalisis totalfenolik. Hasil penelitian menunjukkan bahwa ekstrak etanol daun salam memiliki aktivitas antioksidan dengannilai IC50 sebesar 31,14 ppm dengan total fenolik sebesar 111,89 mg GAE/g. Ekstrak etanol daun kelormemiliki aktivitas antioksidan dengan nilai IC50 sebesar 71,27 ppm dengan total fenolik sebesar 17,12 mgGAE/g. Kombinasi ekstrak etanol daun salam dan ...

FITOFARMAKA: Jurnal Ilmiah Farmasi

Alpha Hydroxy Acid (AHA) adalah asam organik yang terdiri dari 2 (dua) rantai karbon atau lebih y... more Alpha Hydroxy Acid (AHA) adalah asam organik yang terdiri dari 2 (dua) rantai karbon atau lebih yang semakin panjang rantai karbonnya akan semakin besar berat molekulnya. AHA adalah kelas senyawa kimia yang sering digunakan dalam kosmetik dan dermatologi. Beberapa buah dilaporkan mengandung senyawa AHA yaitu, asam sitrat dalam buah jeruk dan nanas, asam glikolat dalam tebu, asam laktat dalam tomat, asam malat dalam apel dan anggur, dan asam tartrat dalam anggur. Penelitian ini dilakukan untuk menetapkan kadar AHA yang terkandung dalam buah ceremai (Phyllanthus acidus L.) dengan menggunakan metode Kromatografi Cair Kinerja Tinggi (KCKT). Sari buah P.acidus dikeringkan dengan metode pengeringan beku. Kemudian senyawa AHA dalam ekstrak kering P.acidus yaitu asam glikolat dan asam sitrat dianalisis menggunakan KCKT dengan jenis kolom Capcell PAK C18 UG120S-5mm(4,6 X 250 mm); detektor UV Vis 210 nm; kecepatan alir 0,5 ml/menit; fase gerak asam fosfat dan asetonitril. Hasil penetapan kada...

Jurnal Farmasi dan Ilmu Kefarmasian Indonesia, Apr 26, 2022

Background: Kidney stones are one of the causes of chronic and acute kidney failure symptoms. The... more Background: Kidney stones are one of the causes of chronic and acute kidney failure symptoms. The flavonoid compounds in Chromolaena odorata leave extract are thought to dissolve calcium in kidney stones. Objective: This study aims to determine the activity of C. odorata leaves extract as a dissolution agent for calcium kidney stones and to characterize the active extract with a liquid chromatograph mass spectrometry. Methods: The leaves of C. odorata were extracted by ultrasonication method using 3 solvents in stages, namely n-hexane, ethyl acetate, and methanol. The powder for kidney stones was immersed in an extract solution of n-hexane, ethyl acetate, and methanol for 5 hours at 37°C. The reaction results were analyzed for their absorbance using a UV-Vis spectrophotometer. The fraction with the best activity was analyzed for phytochemical content with various typical reagents and LCMS/MS. Results: Methanol extract of C. odorata with a concentration of 10,000 g/mL can reduce calcium in kidney stones by 19,2 µg/mL. Based on phytochemical tests and LCMS/MS analysis, the methanol extract of C. odorata leaves contains compounds of the tannins, alkaloids, flavonoids, and steroids. Chromatogram at a retention time of 7.