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Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi Chinese Journal of Parasitology Parasitic Diseases, 2006
To study the bioactive components in ticks which inhibit platelet aggregation, and to understand ... more To study the bioactive components in ticks which inhibit platelet aggregation, and to understand the molecular mechanism of tick-host interaction. Sephadex G-50 gel filtration and high performance liquid chromatography (HPLC) were used to purify the platelet aggregation inhibitor from lxodes sinensis. Its molecular weight and purity were checked by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Platelet-rich plasma (PRP) of rabbit was used to examine the function of platelet aggregation inhibitor. A purified platelet aggregation inhibitor was identified from L. sinensis with a molecular weight of 8 065. It inhibited platelet aggregation induced by ADP with strong potency. The inhibition of platelet aggregation reached over 90% under a concentration of 10 microg/ml. An inhibitor of platelet aggregation from L. sinensis was identified, which may play an important role for ticks to successfully get blood meal from their hosts.
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Lecture Notes in Computer Science, 2010
Aiming to the disadvantages of short-term load forecasting with HHT such as mode mixing and rando... more Aiming to the disadvantages of short-term load forecasting with HHT such as mode mixing and random component, a new short-term load forecasting model based on HHT and ANN is proposed. The first order difference algorithm is adopted to eliminate mode mixing. The random component is forecast with different methods including BP, RBF, SVM, linear and ANN combination. Other components can
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ABSTRACT
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ABSTRACT
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ABSTRACT
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Fire Safety Science, 2005
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Fire Safety Science, 2003
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Wei sheng yan jiu = Journal of hygiene research
To clone the gene of arginine kinase (AK) from Periplaneta americana, produce its recombinant pro... more To clone the gene of arginine kinase (AK) from Periplaneta americana, produce its recombinant protein and investigate its allergenicity. The cDNA of AK was cloned using specific primers from the total RNA of P. americana. The cloned gene was inserted into pMD18-T vector and digested by BamHI and HindIII. The cDNA was sequenced and subcloned into pET-28a expression vector. The cloned AK cDNA gene was expressed in Escherichia coli BL21 (DE3) by IPTG induction. The recombinant AK (rAK) was purified by metal (Ni2+) chelating affinity chromatography. Its allergenicity was examined by both Western blotting and enzyme-linked immunosorbent assay (ELISA). The cloned cDNA ORF sequence (Accession no. EU429466) contained 1068 bp and encoded 365 amino acids. Its sequence homology with the published one (Accession no. AY563004) was 99.9% at nucleotide level. The allergen rAK was highly expressed in E. coli BL21 (DE3) as a soluble protein mainly with the molecular weight of about Mr 45000 under induction of IPTG and purified by 6-His-tag purification system. Both in the non-denaturalization and denaturalization conditions, the recombinant allergen was identified as its affinity to IgE antibodies from the cockroach-allergic patient sera by Western blotting and ELISA. The recombinant cockroach arginine kinase has been obtained with proper allergenicity.
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Fire Protection of a Restaurant Cooking Area with Water Mist Suppression Systems Z. LIU and AK KI... more Fire Protection of a Restaurant Cooking Area with Water Mist Suppression Systems Z. LIU and AK KIM INTRODUCTION In recent years, the development of high-efficiency cooking equipment with high energy input rates and the widespread use of vegetable oils with a high burning ...
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Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi Chinese Journal of Parasitology Parasitic Diseases, 2006
To study the bioactive components in ticks which inhibit platelet aggregation, and to understand ... more To study the bioactive components in ticks which inhibit platelet aggregation, and to understand the molecular mechanism of tick-host interaction. Sephadex G-50 gel filtration and high performance liquid chromatography (HPLC) were used to purify the platelet aggregation inhibitor from lxodes sinensis. Its molecular weight and purity were checked by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Platelet-rich plasma (PRP) of rabbit was used to examine the function of platelet aggregation inhibitor. A purified platelet aggregation inhibitor was identified from L. sinensis with a molecular weight of 8 065. It inhibited platelet aggregation induced by ADP with strong potency. The inhibition of platelet aggregation reached over 90% under a concentration of 10 microg/ml. An inhibitor of platelet aggregation from L. sinensis was identified, which may play an important role for ticks to successfully get blood meal from their hosts.
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Lecture Notes in Computer Science, 2010
Aiming to the disadvantages of short-term load forecasting with HHT such as mode mixing and rando... more Aiming to the disadvantages of short-term load forecasting with HHT such as mode mixing and random component, a new short-term load forecasting model based on HHT and ANN is proposed. The first order difference algorithm is adopted to eliminate mode mixing. The random component is forecast with different methods including BP, RBF, SVM, linear and ANN combination. Other components can
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ABSTRACT
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ABSTRACT
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ABSTRACT
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Fire Safety Science, 2005
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Fire Safety Science, 2003
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Wei sheng yan jiu = Journal of hygiene research
To clone the gene of arginine kinase (AK) from Periplaneta americana, produce its recombinant pro... more To clone the gene of arginine kinase (AK) from Periplaneta americana, produce its recombinant protein and investigate its allergenicity. The cDNA of AK was cloned using specific primers from the total RNA of P. americana. The cloned gene was inserted into pMD18-T vector and digested by BamHI and HindIII. The cDNA was sequenced and subcloned into pET-28a expression vector. The cloned AK cDNA gene was expressed in Escherichia coli BL21 (DE3) by IPTG induction. The recombinant AK (rAK) was purified by metal (Ni2+) chelating affinity chromatography. Its allergenicity was examined by both Western blotting and enzyme-linked immunosorbent assay (ELISA). The cloned cDNA ORF sequence (Accession no. EU429466) contained 1068 bp and encoded 365 amino acids. Its sequence homology with the published one (Accession no. AY563004) was 99.9% at nucleotide level. The allergen rAK was highly expressed in E. coli BL21 (DE3) as a soluble protein mainly with the molecular weight of about Mr 45000 under induction of IPTG and purified by 6-His-tag purification system. Both in the non-denaturalization and denaturalization conditions, the recombinant allergen was identified as its affinity to IgE antibodies from the cockroach-allergic patient sera by Western blotting and ELISA. The recombinant cockroach arginine kinase has been obtained with proper allergenicity.
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Fire Protection of a Restaurant Cooking Area with Water Mist Suppression Systems Z. LIU and AK KI... more Fire Protection of a Restaurant Cooking Area with Water Mist Suppression Systems Z. LIU and AK KIM INTRODUCTION In recent years, the development of high-efficiency cooking equipment with high energy input rates and the widespread use of vegetable oils with a high burning ...
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