zhigang liu - Profile on Academia.edu (original) (raw)
Papers by zhigang liu
[Isolation and purification of an inhibitor on platelet aggregation from Ixodes sinensis]
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi Chinese Journal of Parasitology Parasitic Diseases, 2006
To study the bioactive components in ticks which inhibit platelet aggregation, and to understand ... more To study the bioactive components in ticks which inhibit platelet aggregation, and to understand the molecular mechanism of tick-host interaction. Sephadex G-50 gel filtration and high performance liquid chromatography (HPLC) were used to purify the platelet aggregation inhibitor from lxodes sinensis. Its molecular weight and purity were checked by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Platelet-rich plasma (PRP) of rabbit was used to examine the function of platelet aggregation inhibitor. A purified platelet aggregation inhibitor was identified from L. sinensis with a molecular weight of 8 065. It inhibited platelet aggregation induced by ADP with strong potency. The inhibition of platelet aggregation reached over 90% under a concentration of 10 microg/ml. An inhibitor of platelet aggregation from L. sinensis was identified, which may play an important role for ticks to successfully get blood meal from their hosts.
A New Short-Term Load Forecasting Model of Power System Based on HHT and ANN
Lecture Notes in Computer Science, 2010
Aiming to the disadvantages of short-term load forecasting with HHT such as mode mixing and rando... more Aiming to the disadvantages of short-term load forecasting with HHT such as mode mixing and random component, a new short-term load forecasting model based on HHT and ANN is proposed. The first order difference algorithm is adopted to eliminate mode mixing. The random component is forecast with different methods including BP, RBF, SVM, linear and ANN combination. Other components can
Inergen fire suppression testing
ABSTRACT
Comparison of two different types of water mist systems for fire suppression
ABSTRACT
Full-scale fire testing of water mist fire suppression systems
ABSTRACT
Fire Safety Science, 2005
Large oil fires occurring in industrial oil cookers are very challenging to extinguish due to the... more Large oil fires occurring in industrial oil cookers are very challenging to extinguish due to their size and the large amount of hot oil involved. This paper reports a study to use water mist for large industrial oil cooker protection. The extinguishing mechanisms of water mist and corresponding criteria required for extinguishing large pool cooking oil fires were investigated both theoretically and experimentally. Based on the extinguishing mechanisms and required criteria, two water mist systems were developed in the present work. A series of full-scale fire tests were conducted in a large industrial oil cooker mock-up. The study showed that the two water mist systems presently developed worked effectively to extinguish large cooking oil fires and prevented them from re-igniting. Their extinguishing performance was determined by the type of water mist system, discharge pressure and hood position in the oil cooker.
Fire Safety Science, 2003
This paper presents a distributed fiber optic Brillouin scattering investigation, which involves ... more This paper presents a distributed fiber optic Brillouin scattering investigation, which involves measuring the Brillouin intensity versus time for real-time temperature measurements. This can be applied in practical fire detection schemes through the use of a threshold temperature that corresponds to the maximum Brillouin intensity. In the experiments, a 11 km fiber length was interrogated by 20 ns pulses (2 m spatial resolution) to monitor the temperature variations in a 10 m fiber region. The corresponding theory has confirmed the suitability of real-time intensity monitoring for temperature measurement.
[Cloning, expression and purification of allergen parvalbumin from Aristichthys nobilis and its allergic activity]
Wei sheng yan jiu = Journal of hygiene research
To clone the gene of arginine kinase (AK) from Periplaneta americana, produce its recombinant pro... more To clone the gene of arginine kinase (AK) from Periplaneta americana, produce its recombinant protein and investigate its allergenicity. The cDNA of AK was cloned using specific primers from the total RNA of P. americana. The cloned gene was inserted into pMD18-T vector and digested by BamHI and HindIII. The cDNA was sequenced and subcloned into pET-28a expression vector. The cloned AK cDNA gene was expressed in Escherichia coli BL21 (DE3) by IPTG induction. The recombinant AK (rAK) was purified by metal (Ni2+) chelating affinity chromatography. Its allergenicity was examined by both Western blotting and enzyme-linked immunosorbent assay (ELISA). The cloned cDNA ORF sequence (Accession no. EU429466) contained 1068 bp and encoded 365 amino acids. Its sequence homology with the published one (Accession no. AY563004) was 99.9% at nucleotide level. The allergen rAK was highly expressed in E. coli BL21 (DE3) as a soluble protein mainly with the molecular weight of about Mr 45000 under induction of IPTG and purified by 6-His-tag purification system. Both in the non-denaturalization and denaturalization conditions, the recombinant allergen was identified as its affinity to IgE antibodies from the cockroach-allergic patient sera by Western blotting and ELISA. The recombinant cockroach arginine kinase has been obtained with proper allergenicity.
Application of Fourier Transform Infrared (FTIR) Spectroscopy to Investigate the Degradation of Cooking Oils during Fire Suppression
Fire protection of a restaurant cooking area with water mist suppression systems
Fire Protection of a Restaurant Cooking Area with Water Mist Suppression Systems Z. LIU and AK KI... more Fire Protection of a Restaurant Cooking Area with Water Mist Suppression Systems Z. LIU and AK KIM INTRODUCTION In recent years, the development of high-efficiency cooking equipment with high energy input rates and the widespread use of vegetable oils with a high burning ...
Web page / page Web Systèmes de détection incendie de tunnels routiers enseignements tirés d'un p... more Web page / page Web Systèmes de détection incendie de tunnels routiers enseignements tirés d'un projet international de recherches Liu, Z. G.; Kashef, A.; Lougheed, G. D.; Debs, A.; Gottuk, D. T.; Almand, K. H. Access and use of this website and the material on it are subject to the Terms and Conditions set forth at Syst è m e s de dé t e c t ion inc e ndie de t unne ls rout ie rs e nse igne m e nt s t iré s d'un proje t int e r nat iona l de re che rche s
International research project on fire-detection technologies in tunnels
ABSTRACT
In full-scale fire suppression tests using HCFC Blend A, halogen acid gases were produced. The ag... more In full-scale fire suppression tests using HCFC Blend A, halogen acid gases were produced. The agent and the halogen acid gases were monitored using the Fourier transform infrared (FTIR) spectroscopic technique during the tests, to determine their time-dependent concentrations in a 121 m3 compartment. Gas samples were also analyzed using gas chromatography and mass spectroscopy (GCIMS) and ion selective electrodes (ISEs) after the tests. The GCIMS results for agent concentrations and the ISE results for the halogen acid concentrations were usually lower than those determined using the FTIR technique. Proper gas sampling and instrument calibration are most important to obtain reliable results for the gas measurement and analysis.
A study to investigate the performance of fire detection systems in roadway tunnels was initiated... more A study to investigate the performance of fire detection systems in roadway tunnels was initiated by the Fire Protection Research Foundation in 1999. A Technical Advisory Group consisting of fire and transportation officials, engineering firms, researchers, and system manufacturers was formed to oversee the project, whose overall goals are: - Investigate the performance attributes of current fire detection technologies for roadway tunnel protection; - Develop performance criteria for fire and smoke detection systems in roadway tunnel applications; - Help optimize the technical specifications and installation requirements for this application. The first phase of this project that was completed recently was a literature review of past and current research and application of fire detection systems for road tunnel protection. Based on this study, a second phase of the research program with the following objectives was launched in November 2005: - Develop appropriate design fire scenario...
Today?s increase in road traffic, changes in vehicle mix, and new rolling stock have resulted in ... more Today?s increase in road traffic, changes in vehicle mix, and new rolling stock have resulted in an increase in the incidence of fatal fires in tunnels. In Europe, recent catastrophic tunnel fires have resulted in loss of life and severe property damage. Reliable and early fire detection in tunnels insures an early warning of a fire incident, allowing for timely activation of emergency systems. As such, detection can make the difference between a manageable fire and one that gets out-of-control [1, 2]. The Fire Protection Research Foundation recently completed an international research project, with the support of private and public-sector organizations to evaluate the performance of various types of detection [3]. The project studied the detection performance of nine fire detection systems representing five types of currently available fire detection technologies (Table 1), including their response times to a fire and ability to locate and monitor a fire in a tunnel, with both labo...
[Isolation and purification of an inhibitor on platelet aggregation from Ixodes sinensis]
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi Chinese Journal of Parasitology Parasitic Diseases, 2006
To study the bioactive components in ticks which inhibit platelet aggregation, and to understand ... more To study the bioactive components in ticks which inhibit platelet aggregation, and to understand the molecular mechanism of tick-host interaction. Sephadex G-50 gel filtration and high performance liquid chromatography (HPLC) were used to purify the platelet aggregation inhibitor from lxodes sinensis. Its molecular weight and purity were checked by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Platelet-rich plasma (PRP) of rabbit was used to examine the function of platelet aggregation inhibitor. A purified platelet aggregation inhibitor was identified from L. sinensis with a molecular weight of 8 065. It inhibited platelet aggregation induced by ADP with strong potency. The inhibition of platelet aggregation reached over 90% under a concentration of 10 microg/ml. An inhibitor of platelet aggregation from L. sinensis was identified, which may play an important role for ticks to successfully get blood meal from their hosts.
A New Short-Term Load Forecasting Model of Power System Based on HHT and ANN
Lecture Notes in Computer Science, 2010
Aiming to the disadvantages of short-term load forecasting with HHT such as mode mixing and rando... more Aiming to the disadvantages of short-term load forecasting with HHT such as mode mixing and random component, a new short-term load forecasting model based on HHT and ANN is proposed. The first order difference algorithm is adopted to eliminate mode mixing. The random component is forecast with different methods including BP, RBF, SVM, linear and ANN combination. Other components can
Inergen fire suppression testing
ABSTRACT
Comparison of two different types of water mist systems for fire suppression
ABSTRACT
Full-scale fire testing of water mist fire suppression systems
ABSTRACT
Fire Safety Science, 2005
Large oil fires occurring in industrial oil cookers are very challenging to extinguish due to the... more Large oil fires occurring in industrial oil cookers are very challenging to extinguish due to their size and the large amount of hot oil involved. This paper reports a study to use water mist for large industrial oil cooker protection. The extinguishing mechanisms of water mist and corresponding criteria required for extinguishing large pool cooking oil fires were investigated both theoretically and experimentally. Based on the extinguishing mechanisms and required criteria, two water mist systems were developed in the present work. A series of full-scale fire tests were conducted in a large industrial oil cooker mock-up. The study showed that the two water mist systems presently developed worked effectively to extinguish large cooking oil fires and prevented them from re-igniting. Their extinguishing performance was determined by the type of water mist system, discharge pressure and hood position in the oil cooker.
Fire Safety Science, 2003
This paper presents a distributed fiber optic Brillouin scattering investigation, which involves ... more This paper presents a distributed fiber optic Brillouin scattering investigation, which involves measuring the Brillouin intensity versus time for real-time temperature measurements. This can be applied in practical fire detection schemes through the use of a threshold temperature that corresponds to the maximum Brillouin intensity. In the experiments, a 11 km fiber length was interrogated by 20 ns pulses (2 m spatial resolution) to monitor the temperature variations in a 10 m fiber region. The corresponding theory has confirmed the suitability of real-time intensity monitoring for temperature measurement.
[Cloning, expression and purification of allergen parvalbumin from Aristichthys nobilis and its allergic activity]
Wei sheng yan jiu = Journal of hygiene research
To clone the gene of arginine kinase (AK) from Periplaneta americana, produce its recombinant pro... more To clone the gene of arginine kinase (AK) from Periplaneta americana, produce its recombinant protein and investigate its allergenicity. The cDNA of AK was cloned using specific primers from the total RNA of P. americana. The cloned gene was inserted into pMD18-T vector and digested by BamHI and HindIII. The cDNA was sequenced and subcloned into pET-28a expression vector. The cloned AK cDNA gene was expressed in Escherichia coli BL21 (DE3) by IPTG induction. The recombinant AK (rAK) was purified by metal (Ni2+) chelating affinity chromatography. Its allergenicity was examined by both Western blotting and enzyme-linked immunosorbent assay (ELISA). The cloned cDNA ORF sequence (Accession no. EU429466) contained 1068 bp and encoded 365 amino acids. Its sequence homology with the published one (Accession no. AY563004) was 99.9% at nucleotide level. The allergen rAK was highly expressed in E. coli BL21 (DE3) as a soluble protein mainly with the molecular weight of about Mr 45000 under induction of IPTG and purified by 6-His-tag purification system. Both in the non-denaturalization and denaturalization conditions, the recombinant allergen was identified as its affinity to IgE antibodies from the cockroach-allergic patient sera by Western blotting and ELISA. The recombinant cockroach arginine kinase has been obtained with proper allergenicity.
Application of Fourier Transform Infrared (FTIR) Spectroscopy to Investigate the Degradation of Cooking Oils during Fire Suppression
Fire protection of a restaurant cooking area with water mist suppression systems
Fire Protection of a Restaurant Cooking Area with Water Mist Suppression Systems Z. LIU and AK KI... more Fire Protection of a Restaurant Cooking Area with Water Mist Suppression Systems Z. LIU and AK KIM INTRODUCTION In recent years, the development of high-efficiency cooking equipment with high energy input rates and the widespread use of vegetable oils with a high burning ...
Web page / page Web Systèmes de détection incendie de tunnels routiers enseignements tirés d'un p... more Web page / page Web Systèmes de détection incendie de tunnels routiers enseignements tirés d'un projet international de recherches Liu, Z. G.; Kashef, A.; Lougheed, G. D.; Debs, A.; Gottuk, D. T.; Almand, K. H. Access and use of this website and the material on it are subject to the Terms and Conditions set forth at Syst è m e s de dé t e c t ion inc e ndie de t unne ls rout ie rs e nse igne m e nt s t iré s d'un proje t int e r nat iona l de re che rche s
International research project on fire-detection technologies in tunnels
ABSTRACT
In full-scale fire suppression tests using HCFC Blend A, halogen acid gases were produced. The ag... more In full-scale fire suppression tests using HCFC Blend A, halogen acid gases were produced. The agent and the halogen acid gases were monitored using the Fourier transform infrared (FTIR) spectroscopic technique during the tests, to determine their time-dependent concentrations in a 121 m3 compartment. Gas samples were also analyzed using gas chromatography and mass spectroscopy (GCIMS) and ion selective electrodes (ISEs) after the tests. The GCIMS results for agent concentrations and the ISE results for the halogen acid concentrations were usually lower than those determined using the FTIR technique. Proper gas sampling and instrument calibration are most important to obtain reliable results for the gas measurement and analysis.
A study to investigate the performance of fire detection systems in roadway tunnels was initiated... more A study to investigate the performance of fire detection systems in roadway tunnels was initiated by the Fire Protection Research Foundation in 1999. A Technical Advisory Group consisting of fire and transportation officials, engineering firms, researchers, and system manufacturers was formed to oversee the project, whose overall goals are: - Investigate the performance attributes of current fire detection technologies for roadway tunnel protection; - Develop performance criteria for fire and smoke detection systems in roadway tunnel applications; - Help optimize the technical specifications and installation requirements for this application. The first phase of this project that was completed recently was a literature review of past and current research and application of fire detection systems for road tunnel protection. Based on this study, a second phase of the research program with the following objectives was launched in November 2005: - Develop appropriate design fire scenario...
Today?s increase in road traffic, changes in vehicle mix, and new rolling stock have resulted in ... more Today?s increase in road traffic, changes in vehicle mix, and new rolling stock have resulted in an increase in the incidence of fatal fires in tunnels. In Europe, recent catastrophic tunnel fires have resulted in loss of life and severe property damage. Reliable and early fire detection in tunnels insures an early warning of a fire incident, allowing for timely activation of emergency systems. As such, detection can make the difference between a manageable fire and one that gets out-of-control [1, 2]. The Fire Protection Research Foundation recently completed an international research project, with the support of private and public-sector organizations to evaluate the performance of various types of detection [3]. The project studied the detection performance of nine fire detection systems representing five types of currently available fire detection technologies (Table 1), including their response times to a fire and ability to locate and monitor a fire in a tunnel, with both labo...