Agustina Schenone | INTEC - Academia.edu (original) (raw)
Uploads
Papers by Agustina Schenone
South Florida Journal of Environmental and Animal Science
La necesidad de producir más alimentos ha llevado al aumento del uso de pesticidas, entre ellos g... more La necesidad de producir más alimentos ha llevado al aumento del uso de pesticidas, entre ellos glifosato, el cual es ampliamente empleado en la producción de soja transgénica. Esto ha implicado que crezcan los casos de intoxicaciones y contaminación de recursos naturales. Por tal motivo los entes gubernamentales han formulado instrucciones de manipulación y de descarte de los envases comerciales. El objetivo del presente trabajo fue evaluar la capacidad de Candida tropicalis LMFIQ 703 para disminuir la concentración de glifosato en el tercer enjuague de bidones y así reducir el riesgo de impacto ambiental adverso que producen los residuos de pesticida en los envases vacíos almacenados por largos periodos de tiempo. Se sembraron suspensiones de levadura sin adaptación, en soluciones de Credit® Amonio (Ingrediente activo: sal amónica de la N-fosfonometil glicina) con concentración conocida (similar a la del tercer enjuague). Se incubó a 28°C durante 28 días y se realizó el recuento m...
Environmental Science and Pollution Research, 2019
A theoretical and experimental study of bisphenol A (BPA) degradation by the UV/H2O2 process in w... more A theoretical and experimental study of bisphenol A (BPA) degradation by the UV/H2O2 process in water is presented. The effects of the H2O2 concentration and the specific rate of photon emission (EP,0) on BPA degradation were investigated. A kinetic model derived from a reaction sequence was employed to predict BPA and hydrogen peroxide concentrations over time using an annular photochemical reactor in batch recirculation mode. The local volumetric rate of photon absorption (LVRPA) inside the photoreactor was computed using a Line Source with Parallel Plane emission model (LSPP). From the proposed kinetic model and the experimental data, the second order rate constants of the reactions between hydroxyl radicals and the main reacting species (H2O2 and BPA) were estimated applying a nonlinear regression method. A good agreement between the kinetic model and experimental data, for a wide range of operating conditions, was obtained. For BPA, H2O2, and TOC concentrations, the calculated root means square errors (RMSE) were 2.3 × 10− 2, 9.8 × 10− 1, and 9.0 × 10− 2 mmol L− 1, respectively. The simplified kinetic model presented in this work can be directly applied to scaling-up and reactor design, since the estimated kinetic constants are independent of the reactor size, shape, and configuration. Further experiments were made by employing low BPA initial concentration (100 μg L− 1) in water and real wastewater. A lower degradation rate of BPA was observed in the real wastewater, although the UV/H2O2 process has also been able to completely degrade the target pollutant in less than 1 h.
Environmental Science and Pollution Research, 2021
A Correction to this paper has been published: 10.1007/s11356-021-15050-7
ochratoxin A) using fluorescence emission-excitation matrices and multivariate calibration
Fil: Schenone, Agustina Violeta. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Cent... more Fil: Schenone, Agustina Violeta. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Desarrollo Tecnologico para la Industria Quimica. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnologico para la Industria Quimica; Argentina
Analytica Chimica Acta, 2015
A new residual modeling algorithm for nonbilinear data is presented, namely unfolded partial leas... more A new residual modeling algorithm for nonbilinear data is presented, namely unfolded partial least squares with interference modeling of non bilinear data by multivariate curve resolution by alternating least squares (U-PLS/IMNB/MCR-ALS). Nonbilinearity represents a challenging data structure problem to achieve analyte quantitation from second-order data in the presence of uncalibrated components. Total synchronous fluorescence spectroscopy (TSFS) generates matrices which constitute a typical example of this kind of data. Although the nonbilinear profile of the interferent can be achieved by modeling TSFS data with unfolded partial least squares with residual bilinearization (U-PLS/RBL), an extremely large number of RBL factors has to be considered. Simulated data show that the new model can conveniently handle the studied analytical problem with better performance than PARAFAC, U-PLS/RBL and MCR-ALS, the latter modeling the unfolded data. Besides, one example involving TSFS real matrices illustrates the ability of the new method to handle experimental data, which consists in the determination of ciprofloxacin in the presence of norfloxacin as interferent in water samples.
Journal of Chromatography A, 2009
A fast chromatographic methodology is presented for the analysis of three synthetic dyes in non-a... more A fast chromatographic methodology is presented for the analysis of three synthetic dyes in non-alcoholic beverages: amaranth (E123), sunset yellow FCF (E110) and tartrazine (E102). Seven soft drinks (purchased from a local supermarket) were homogenized, filtered and injected into the chromatographic system. Second order data were obtained by a rapid LC separation and DAD detection. A comparative study of the performance of two second order algorithms (MCR-ALS and U-PLS/RBL) applied to model the data, is presented. Interestingly, the data present time shift between different chromatograms and cannot be conveniently corrected to determine the above-mentioned dyes in beverage samples. This fact originates the lack of trilinearity that cannot be conveniently pre-processed and can hardly be modelled by using U-PLS/RBL algorithm. On the contrary, MCR-ALS has shown to be an excellent tool for modelling this kind of data allowing to reach acceptable figures of merit. Recovery values ranged between 97% and 105% when analyzing artificial and real samples were indicative of the good performance of the method. In contrast with the complete separation, which consumes 10 mL of methanol and 3 mL of 0.08 mol L -1 ammonium acetate, the proposed fast chromatography method requires only 0.46 mL of methanol and 1.54 mL of 0.08 mol L -1 ammonium acetate. Consequently, analysis time could be reduced up to 14.2% of the necessary time to perform the complete separation allowing saving both solvents and time, which are related to a reduction of both the costs per analysis and environmental impact.
Analytical and Bioanalytical Chemistry, 2013
In this work, we present the development of a method for the determination of doxorubicin in plas... more In this work, we present the development of a method for the determination of doxorubicin in plasma samples in the presence of an unexpected component (riboflavin) by using total synchronous fluorescence spectroscopic data matrices. To the best of our knowledge, this is the first time that the second-order advantage is obtained with this kind of data. Two strategies including unfolding the data and: (a) processing with multivariate curve resolution coupled to alternating least-squares as first-order data or (b) processing with unfolded partial least-squares and exploiting the second-order advantage by the residual bilinearization procedure were considered. The calibration set was built with human plasma samples spiked with doxorubicin, while the validation set was prepared with human plasma samples spiked with both doxorubicin and riboflavin, a drug whose spectrum highly overlaps with the one corresponding to doxorubicin. Both methodologies reached good indicators of accuracy: recoveries of ca. 100 ± 8% and REP of ca. 5%; and precision: coefficient of variations between 7 and 9%.
Talanta, 2011
Benzo[a]pyrene (B[a]P) is a prototypic carcinogenic polycyclic aromatic hydrocarbon (PAH), which ... more Benzo[a]pyrene (B[a]P) is a prototypic carcinogenic polycyclic aromatic hydrocarbon (PAH), which requires metabolic activation to produce its detrimental effects. Measurement of B[a]P metabolites in human urine could provide a direct way to assess individual differences in susceptibility to PAH-related cancer. This article focuses on the development of screening methodology for the routine analysis of B[a]P metabolites in urine samples. It explores the solid-surface room-temperature fluorescence (RTF) properties of 3-hydroxybenzo[a]pyrene, benzo[a]pyrene-trans-9,10-dihydrodiol, benzo[a]pyrene-r-7,t-8,c-9-tetrahydrotriol and benzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol previously extracted from urine samples with octadecyl-silica membranes. Relative standard deviations varying from 2.1% (benzo[a]pyrene-r-7,t-8,c-9-tetrahydrotriol) to 8.6% (3-hydroxy-benzo[a]pyrene) are obtained with the aid of fiber optic probe that eliminates the need for manual optimization of signal intensities. Analytical recoveries from human urine samples varied from 87.5 ± 3.1% (3-hydroxy-benzo[a]pyrene) to 99.8 ± 2.5% (benzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol). The excellent analytical figures of merit and the simplicity of the experimental procedure demonstrate the potential of this approach for screening biomarkers of PAH exposure in numerous urine samples.
Environmental Science and Pollution Research, 2021
A Correction to this paper has been published: https://doi.org/10.1007/s11356-021-15050-7
Computer Aided Chemical Engineering
Environmental Science and Pollution Research
Journal of Photochemistry and Photobiology A: Chemistry
Environmental Science and Pollution Research
A kinetic model describing Fenton and photo-Fenton degradation of paracetamol (PCT) and consumpti... more A kinetic model describing Fenton and photo-Fenton degradation of paracetamol (PCT) and consumption of hydrogen peroxide (H2O2) was proposed. A set of Fenton and photo-Fenton experiments (18 runs in total) was performed by fixing the initial concentration of PCT to 40 mg L−1 and varying the initial concentrations of H2O2 and ferrous ion, Fe2+. The experimental set-up was a well-stirred annular photoreactor equipped with an actinic BL TL-DK 36 W/10 1SL lamp. Experimental results highlighted that PCT is no more detected by HPLC analysis within a minimum reaction time of 2.5 and a maximum reaction time of 15.0 min. Besides, a maximum conversion of total organic carbon (TOC) of 68.5% was observed after 75 min of reaction in case of using UV radiation and the highest concentrations of the Fenton reagents. The experimental data were used to fit the kinetic model. The radiation field inside the reactor was taken into account through the local volumetric rate of photon absorption, evaluated...
South Florida Journal of Environmental and Animal Science
La necesidad de producir más alimentos ha llevado al aumento del uso de pesticidas, entre ellos g... more La necesidad de producir más alimentos ha llevado al aumento del uso de pesticidas, entre ellos glifosato, el cual es ampliamente empleado en la producción de soja transgénica. Esto ha implicado que crezcan los casos de intoxicaciones y contaminación de recursos naturales. Por tal motivo los entes gubernamentales han formulado instrucciones de manipulación y de descarte de los envases comerciales. El objetivo del presente trabajo fue evaluar la capacidad de Candida tropicalis LMFIQ 703 para disminuir la concentración de glifosato en el tercer enjuague de bidones y así reducir el riesgo de impacto ambiental adverso que producen los residuos de pesticida en los envases vacíos almacenados por largos periodos de tiempo. Se sembraron suspensiones de levadura sin adaptación, en soluciones de Credit® Amonio (Ingrediente activo: sal amónica de la N-fosfonometil glicina) con concentración conocida (similar a la del tercer enjuague). Se incubó a 28°C durante 28 días y se realizó el recuento m...
Environmental Science and Pollution Research, 2019
A theoretical and experimental study of bisphenol A (BPA) degradation by the UV/H2O2 process in w... more A theoretical and experimental study of bisphenol A (BPA) degradation by the UV/H2O2 process in water is presented. The effects of the H2O2 concentration and the specific rate of photon emission (EP,0) on BPA degradation were investigated. A kinetic model derived from a reaction sequence was employed to predict BPA and hydrogen peroxide concentrations over time using an annular photochemical reactor in batch recirculation mode. The local volumetric rate of photon absorption (LVRPA) inside the photoreactor was computed using a Line Source with Parallel Plane emission model (LSPP). From the proposed kinetic model and the experimental data, the second order rate constants of the reactions between hydroxyl radicals and the main reacting species (H2O2 and BPA) were estimated applying a nonlinear regression method. A good agreement between the kinetic model and experimental data, for a wide range of operating conditions, was obtained. For BPA, H2O2, and TOC concentrations, the calculated root means square errors (RMSE) were 2.3 × 10− 2, 9.8 × 10− 1, and 9.0 × 10− 2 mmol L− 1, respectively. The simplified kinetic model presented in this work can be directly applied to scaling-up and reactor design, since the estimated kinetic constants are independent of the reactor size, shape, and configuration. Further experiments were made by employing low BPA initial concentration (100 μg L− 1) in water and real wastewater. A lower degradation rate of BPA was observed in the real wastewater, although the UV/H2O2 process has also been able to completely degrade the target pollutant in less than 1 h.
Environmental Science and Pollution Research, 2021
A Correction to this paper has been published: 10.1007/s11356-021-15050-7
ochratoxin A) using fluorescence emission-excitation matrices and multivariate calibration
Fil: Schenone, Agustina Violeta. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Cent... more Fil: Schenone, Agustina Violeta. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Desarrollo Tecnologico para la Industria Quimica. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnologico para la Industria Quimica; Argentina
Analytica Chimica Acta, 2015
A new residual modeling algorithm for nonbilinear data is presented, namely unfolded partial leas... more A new residual modeling algorithm for nonbilinear data is presented, namely unfolded partial least squares with interference modeling of non bilinear data by multivariate curve resolution by alternating least squares (U-PLS/IMNB/MCR-ALS). Nonbilinearity represents a challenging data structure problem to achieve analyte quantitation from second-order data in the presence of uncalibrated components. Total synchronous fluorescence spectroscopy (TSFS) generates matrices which constitute a typical example of this kind of data. Although the nonbilinear profile of the interferent can be achieved by modeling TSFS data with unfolded partial least squares with residual bilinearization (U-PLS/RBL), an extremely large number of RBL factors has to be considered. Simulated data show that the new model can conveniently handle the studied analytical problem with better performance than PARAFAC, U-PLS/RBL and MCR-ALS, the latter modeling the unfolded data. Besides, one example involving TSFS real matrices illustrates the ability of the new method to handle experimental data, which consists in the determination of ciprofloxacin in the presence of norfloxacin as interferent in water samples.
Journal of Chromatography A, 2009
A fast chromatographic methodology is presented for the analysis of three synthetic dyes in non-a... more A fast chromatographic methodology is presented for the analysis of three synthetic dyes in non-alcoholic beverages: amaranth (E123), sunset yellow FCF (E110) and tartrazine (E102). Seven soft drinks (purchased from a local supermarket) were homogenized, filtered and injected into the chromatographic system. Second order data were obtained by a rapid LC separation and DAD detection. A comparative study of the performance of two second order algorithms (MCR-ALS and U-PLS/RBL) applied to model the data, is presented. Interestingly, the data present time shift between different chromatograms and cannot be conveniently corrected to determine the above-mentioned dyes in beverage samples. This fact originates the lack of trilinearity that cannot be conveniently pre-processed and can hardly be modelled by using U-PLS/RBL algorithm. On the contrary, MCR-ALS has shown to be an excellent tool for modelling this kind of data allowing to reach acceptable figures of merit. Recovery values ranged between 97% and 105% when analyzing artificial and real samples were indicative of the good performance of the method. In contrast with the complete separation, which consumes 10 mL of methanol and 3 mL of 0.08 mol L -1 ammonium acetate, the proposed fast chromatography method requires only 0.46 mL of methanol and 1.54 mL of 0.08 mol L -1 ammonium acetate. Consequently, analysis time could be reduced up to 14.2% of the necessary time to perform the complete separation allowing saving both solvents and time, which are related to a reduction of both the costs per analysis and environmental impact.
Analytical and Bioanalytical Chemistry, 2013
In this work, we present the development of a method for the determination of doxorubicin in plas... more In this work, we present the development of a method for the determination of doxorubicin in plasma samples in the presence of an unexpected component (riboflavin) by using total synchronous fluorescence spectroscopic data matrices. To the best of our knowledge, this is the first time that the second-order advantage is obtained with this kind of data. Two strategies including unfolding the data and: (a) processing with multivariate curve resolution coupled to alternating least-squares as first-order data or (b) processing with unfolded partial least-squares and exploiting the second-order advantage by the residual bilinearization procedure were considered. The calibration set was built with human plasma samples spiked with doxorubicin, while the validation set was prepared with human plasma samples spiked with both doxorubicin and riboflavin, a drug whose spectrum highly overlaps with the one corresponding to doxorubicin. Both methodologies reached good indicators of accuracy: recoveries of ca. 100 ± 8% and REP of ca. 5%; and precision: coefficient of variations between 7 and 9%.
Talanta, 2011
Benzo[a]pyrene (B[a]P) is a prototypic carcinogenic polycyclic aromatic hydrocarbon (PAH), which ... more Benzo[a]pyrene (B[a]P) is a prototypic carcinogenic polycyclic aromatic hydrocarbon (PAH), which requires metabolic activation to produce its detrimental effects. Measurement of B[a]P metabolites in human urine could provide a direct way to assess individual differences in susceptibility to PAH-related cancer. This article focuses on the development of screening methodology for the routine analysis of B[a]P metabolites in urine samples. It explores the solid-surface room-temperature fluorescence (RTF) properties of 3-hydroxybenzo[a]pyrene, benzo[a]pyrene-trans-9,10-dihydrodiol, benzo[a]pyrene-r-7,t-8,c-9-tetrahydrotriol and benzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol previously extracted from urine samples with octadecyl-silica membranes. Relative standard deviations varying from 2.1% (benzo[a]pyrene-r-7,t-8,c-9-tetrahydrotriol) to 8.6% (3-hydroxy-benzo[a]pyrene) are obtained with the aid of fiber optic probe that eliminates the need for manual optimization of signal intensities. Analytical recoveries from human urine samples varied from 87.5 ± 3.1% (3-hydroxy-benzo[a]pyrene) to 99.8 ± 2.5% (benzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol). The excellent analytical figures of merit and the simplicity of the experimental procedure demonstrate the potential of this approach for screening biomarkers of PAH exposure in numerous urine samples.
Environmental Science and Pollution Research, 2021
A Correction to this paper has been published: https://doi.org/10.1007/s11356-021-15050-7
Computer Aided Chemical Engineering
Environmental Science and Pollution Research
Journal of Photochemistry and Photobiology A: Chemistry
Environmental Science and Pollution Research
A kinetic model describing Fenton and photo-Fenton degradation of paracetamol (PCT) and consumpti... more A kinetic model describing Fenton and photo-Fenton degradation of paracetamol (PCT) and consumption of hydrogen peroxide (H2O2) was proposed. A set of Fenton and photo-Fenton experiments (18 runs in total) was performed by fixing the initial concentration of PCT to 40 mg L−1 and varying the initial concentrations of H2O2 and ferrous ion, Fe2+. The experimental set-up was a well-stirred annular photoreactor equipped with an actinic BL TL-DK 36 W/10 1SL lamp. Experimental results highlighted that PCT is no more detected by HPLC analysis within a minimum reaction time of 2.5 and a maximum reaction time of 15.0 min. Besides, a maximum conversion of total organic carbon (TOC) of 68.5% was observed after 75 min of reaction in case of using UV radiation and the highest concentrations of the Fenton reagents. The experimental data were used to fit the kinetic model. The radiation field inside the reactor was taken into account through the local volumetric rate of photon absorption, evaluated...