Burcu Şen | Istanbul University (original) (raw)

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Papers by Burcu Şen

Planta Medica

The pathophysiology of Parkinson's disease(PD) is linked to accumulation of the cytoplasmic struc... more The pathophysiology of Parkinson's disease(PD) is linked to accumulation of the cytoplasmic structures called Lewy bodies with their main constituent of alpha-synuclein oligomers. Recent studies showed that α-synuclein overexpression is associated with microglial activation, neuroinflammation, and neurodegeneration. In order to protect neurons and prevent disease progression, studies on agents that are effective on alpha-synuclein expression, whose oligomers cause cell damage by initiating neuroinflammation, and therefore anti-inflammatory and antioxidant agents, are increasingly important [1].
This study aimed to determine antioxidant, anti-inflammatory, and neuroprotective activities of various extracts of Clematis vitalba, Ecballium elaterium(EE), Fraxinus ornus subsp. ornus(FOO), Paliurus spina-christi and Sambucus ebulus(SE), which might indicate a therapeutic potential for treatment of inflammatory disorders like PD. FOO showed the highest antioxidant activity by DPPH method. FOO, SE and EE extracts exhibited higher anti-inflammatory potential since they reduced nitric oxide and TNF-production by Griess assay (using LPS-activated RAW 264.7 macrophages) and TNF bioassay (using L929 cells), and were found to be rich in flavonoid and phenolic compounds chromatographically. Therefore, their protective activities on the toxicity induced by rotenone and A-53T-α-syn plasmid were tested by analysis of the change in alpha-synuclein expression, its effect on the cell lines and cell viability analysis through xCELLigence. Only FOO cortex extract was protective and decreased the cell death. Only FOO cortex extract was protective and decreased cell death. Our findings indicate that FOO has good anti-inflammatory and neuroprotective activity. Further studies are required to assess its potential applications in the treatment of inflammatory disorders like PD.

Hacettepe University Journal of the Faculty of Pharmacy, 2023

Pathophysiology and Treatment Approaches of Amyotrophic Lateral Sclerosis Review Article ÖZET Ülk... more Pathophysiology and Treatment Approaches of Amyotrophic Lateral Sclerosis Review Article ÖZET Ülkemizde her yıl 1500 ile 4500 kişiye tanısı konulan ALS ilerleyici kas fonksiyon kaybı ile kendini gösteren ölümcül bir nörodejeneratif hastalık olarak bilinmektedir. Günden güne bireylerin kendi temel ihtiyaçlarını dahi tek başına karşılayamadığı bu hastalığın patogenezinde glutamat eksitotoksisitesi ve genetik faktörler en önemli etmenler olarak görülmektedir. Birçok semptomatik ve tedaviye yönelik ilaç araştırmaları devam etse de tedavi protokolünde yalnızca FDA onaylı riluzol ve edaravon yer almaktadır. Bunun yanı sıra hem hastalığın önlenmesi ve tedavi edilmesi hem de semptomların iyileştirilmesi ve hastaların yaşam kalitesinin arttırılmasında hücre bazlı tedavilerden ve fitoterapiden de yararlanılmaktadır. Bu çalışma ile ALS hastalığının patogenezine, tedavisine ve tedavisindeki gelişmelere yer verilerek ilaçların tedavi potansiyelleri incelenmiş ve yeni çalışmalara yol göstermesi amacıyla bir değerlendirme hazırlanmıştır.

Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi, Sep 1, 2014

Planta Medica, Aug 21, 2013

Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi, Sep 1, 2014

South African Journal of Botany, 2021

Zika, dengue, and Yellow fever are transmitted primarily by Aedes aegypti mosquitoes. Investigati... more Zika, dengue, and Yellow fever are transmitted primarily by Aedes aegypti mosquitoes. Investigations to find more natural but highly effective pesticidal agents against Ae. aegypti have increased in the last few years. Plant-based compounds have been the source of several current pesticides and have provided models for creation of synthetic derivatives like the pyrethroids. Plants continue to be a rich source for potential new active ingredients. The aim of this study is to increase knowledge on V. dioscoridis and to present the results of the cytotoxic and insecticidal activities of various extracts of its roots and volatile compound compositions of roots. The hexane and chloroform extracts, HM1 and CM1 respectively, exhibited cytotoxicity with IC50 values of 128.4 and 86.93 µg/mL against the HepG2 cell line. Because CM1 had higher cytotoxicity across the studied concentration range, it was also tested against the HUVEC cell line. Extracts HM1 and CM1 were also tested for their insecticidal activities. CM1 was found to be the more active (mortality (%) = 96.7% ± 5.8 at 5 μg/mosquito), consistent with its cytotoxic activity. Since these active extracts were nonpolar, the chemical composition of the volatile compounds of the roots were analyzed by GC-MS analysis. About 87.7% of the essential oil were characterized. The major compound was ß-eudesmol (9.2%), following by camphene (8.9%), bornyl acetate (8.2%) and maaliol (7.6%). This is the first study on the HepG2 and HUVEC cytotoxicity and insecticidal activity of V. dioscoridis. Further studies on the potential of this plant to define the spectrum of activity and examine potential therapeutic agents is warranted.

International Journal of Secondary Metabolite, 2020

Free radicals are shown as the main reason of many chronic and degenerative diseases. Because of ... more Free radicals are shown as the main reason of many chronic and degenerative diseases. Because of the toxicity and undesirable effects of synthetic antioxidants, finding of new antioxidant natural agents is very important, especially edible plants. As an edible plant, valerian is used for various aims. Also, Valeriana species are known with their antioxidative compounds. In our previous study, some biological activities of Valeriana alliariifolia Adams roots extracts, collected in 2012, and the chemical compositions of active samples were determined. For this study, after 5 years collected plant materials were investigated again to determine and compare the antioxidant activities, the total phenolic contents and the chemical composition profile of the extracts from different plant-parts and to compare obtained results with the previous data. While RWI, RWM1 and RWM1residue were found most active by DPPH method and AHM1 by FRAP method, REM1 showed the highest activity by CUPRAC method. The high activity of AHM1 is parallel to its phenolic content. It can be thought that the difference between the results of our two studies, is due to the change of plant content from year to year and various environmental factors. This is important for achieving standardization in the production of its preparations.

International Journal of Secondary Metabolite, 2020

This study on Acer campestre L. subsp. campestre, used traditionally in treatments of various inf... more This study on Acer campestre L. subsp. campestre, used traditionally in treatments of various inflammation disorders, especially hemorrhoids and rheumatism, aimed to determine the total phenolic contents, the antioxidant and antimicrobial potentials, and the chemical composition profile of the extracts and subextracts from maple syrup and Acer campestre L. subsp. campestre leaves and twigs. The antioxidant activities of maple syrup extracts and subextracts were examined by 2,2-diphenyl-1-picryl-hydrazyl (DPPH), and the total phenolic contents of all samples were determined using the Folin-Ciocalteu reagent (FCR) method. Also, they were tested for their antimicrobial activities by microbroth dilution technique. Catechic tannin, saponins and flavonoids are determined in its leaves and twigs by preliminary qualitative phytochemical analysis. The alcoholic extracts obtained by 48-hour maceration (91.2%), ethyl acetate subextract of alcohol extracts obtained by 24-hour maceration (91.1%) and alcohol extracts obtained by 24-hour maceration (88.8%) exhibited higher radical scavenging activity than other samples, while the decoction and infusion had moderate activities. Beside of this, it was found that the syrup and its subextracts have more total phenolic contents than other extracts. The alcohol extracts obtained by 24-hour maceration and by 48-hour maceration exhibited higher activity against Candida albicans (78 μg/mL and 156 μg/mL, respectively). Only the alcohol extract obtained by 48-hour maceration and ethyl acetate subextract of syrup showed an activity against Escherichia coli, while all samples except butanol subextract of syrup have an antibacterial activity against Pseudomonas aeruginosa.

Istanbul Journal of Pharmacy, 2019

Marmara Pharmaceutical Journal, 2017

African journal of microbiology research

The antimicrobial activities of the different aerial and root extracts of Salvia cilicica were de... more The antimicrobial activities of the different aerial and root extracts of Salvia cilicica were determined aiming to evaluate whether, it can be used in phytotherapy as an antimicrobial agent. In this study, the antimicrobial activity of roots and aerial parts of Salvia cilicica extracts was evaluated using micro dilution and disc diffusion methods against Gram positive and Gram negative reference standard microorganisms and yeast Candida albicans. All of the extracts, with the exception of ethanol extract, showed antimicrobial activity by using MIC and MBC tests. The roots-petroleum ether and acetone extracts showed the highest antimicrobial activity (MIC’s ranges 20-313 μg/ml) against Gram positive bacteria. The root-acetone extract showed higher antimicrobial activity against Gram negative bacteria in comparison to other extracts. The most remarkable result of the antimicrobial activities is that, except for the ethanol extract, the Salvia cilicica had a good inhibitory effect (MI...

Zeitschrift für Naturforschung C, 2012

From the roots of Aconitum vulparia Rchb., collected in Prüm (Germany), a new norditerpenoid alka... more From the roots of Aconitum vulparia Rchb., collected in Prüm (Germany), a new norditerpenoid alkaloid, named alexhumboldtine, has been isolated along with the known norditerpenoid alkaloids lappaconitine, anthranoyllycoctonine, lycoctonine, puberaconitine, ajacine, and septentriodine. The structure of alexhumboldtine was established on the basis of 1H, 13C, DEPT, homonuclear 1H COSY, NOESY, HSQC, and HMBC NMR studies. From the aerial parts of the plant another norditerpenoid alkaloid, aconorine, has been isolated

Osmanlı Bilimi Araştırmaları, 2018

Mersin Universitesi Tıp Fakultesi Lokman Hekim Tıp Tarihi Ve Folklorik Tıp Dergisi, Apr 5, 2014

Planta Medica

The pathophysiology of Parkinson's disease(PD) is linked to accumulation of the cytoplasmic struc... more The pathophysiology of Parkinson's disease(PD) is linked to accumulation of the cytoplasmic structures called Lewy bodies with their main constituent of alpha-synuclein oligomers. Recent studies showed that α-synuclein overexpression is associated with microglial activation, neuroinflammation, and neurodegeneration. In order to protect neurons and prevent disease progression, studies on agents that are effective on alpha-synuclein expression, whose oligomers cause cell damage by initiating neuroinflammation, and therefore anti-inflammatory and antioxidant agents, are increasingly important [1].
This study aimed to determine antioxidant, anti-inflammatory, and neuroprotective activities of various extracts of Clematis vitalba, Ecballium elaterium(EE), Fraxinus ornus subsp. ornus(FOO), Paliurus spina-christi and Sambucus ebulus(SE), which might indicate a therapeutic potential for treatment of inflammatory disorders like PD. FOO showed the highest antioxidant activity by DPPH method. FOO, SE and EE extracts exhibited higher anti-inflammatory potential since they reduced nitric oxide and TNF-production by Griess assay (using LPS-activated RAW 264.7 macrophages) and TNF bioassay (using L929 cells), and were found to be rich in flavonoid and phenolic compounds chromatographically. Therefore, their protective activities on the toxicity induced by rotenone and A-53T-α-syn plasmid were tested by analysis of the change in alpha-synuclein expression, its effect on the cell lines and cell viability analysis through xCELLigence. Only FOO cortex extract was protective and decreased the cell death. Only FOO cortex extract was protective and decreased cell death. Our findings indicate that FOO has good anti-inflammatory and neuroprotective activity. Further studies are required to assess its potential applications in the treatment of inflammatory disorders like PD.

Hacettepe University Journal of the Faculty of Pharmacy, 2023

Pathophysiology and Treatment Approaches of Amyotrophic Lateral Sclerosis Review Article ÖZET Ülk... more Pathophysiology and Treatment Approaches of Amyotrophic Lateral Sclerosis Review Article ÖZET Ülkemizde her yıl 1500 ile 4500 kişiye tanısı konulan ALS ilerleyici kas fonksiyon kaybı ile kendini gösteren ölümcül bir nörodejeneratif hastalık olarak bilinmektedir. Günden güne bireylerin kendi temel ihtiyaçlarını dahi tek başına karşılayamadığı bu hastalığın patogenezinde glutamat eksitotoksisitesi ve genetik faktörler en önemli etmenler olarak görülmektedir. Birçok semptomatik ve tedaviye yönelik ilaç araştırmaları devam etse de tedavi protokolünde yalnızca FDA onaylı riluzol ve edaravon yer almaktadır. Bunun yanı sıra hem hastalığın önlenmesi ve tedavi edilmesi hem de semptomların iyileştirilmesi ve hastaların yaşam kalitesinin arttırılmasında hücre bazlı tedavilerden ve fitoterapiden de yararlanılmaktadır. Bu çalışma ile ALS hastalığının patogenezine, tedavisine ve tedavisindeki gelişmelere yer verilerek ilaçların tedavi potansiyelleri incelenmiş ve yeni çalışmalara yol göstermesi amacıyla bir değerlendirme hazırlanmıştır.

Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi, Sep 1, 2014

Planta Medica, Aug 21, 2013

Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi, Sep 1, 2014

South African Journal of Botany, 2021

Zika, dengue, and Yellow fever are transmitted primarily by Aedes aegypti mosquitoes. Investigati... more Zika, dengue, and Yellow fever are transmitted primarily by Aedes aegypti mosquitoes. Investigations to find more natural but highly effective pesticidal agents against Ae. aegypti have increased in the last few years. Plant-based compounds have been the source of several current pesticides and have provided models for creation of synthetic derivatives like the pyrethroids. Plants continue to be a rich source for potential new active ingredients. The aim of this study is to increase knowledge on V. dioscoridis and to present the results of the cytotoxic and insecticidal activities of various extracts of its roots and volatile compound compositions of roots. The hexane and chloroform extracts, HM1 and CM1 respectively, exhibited cytotoxicity with IC50 values of 128.4 and 86.93 µg/mL against the HepG2 cell line. Because CM1 had higher cytotoxicity across the studied concentration range, it was also tested against the HUVEC cell line. Extracts HM1 and CM1 were also tested for their insecticidal activities. CM1 was found to be the more active (mortality (%) = 96.7% ± 5.8 at 5 μg/mosquito), consistent with its cytotoxic activity. Since these active extracts were nonpolar, the chemical composition of the volatile compounds of the roots were analyzed by GC-MS analysis. About 87.7% of the essential oil were characterized. The major compound was ß-eudesmol (9.2%), following by camphene (8.9%), bornyl acetate (8.2%) and maaliol (7.6%). This is the first study on the HepG2 and HUVEC cytotoxicity and insecticidal activity of V. dioscoridis. Further studies on the potential of this plant to define the spectrum of activity and examine potential therapeutic agents is warranted.

International Journal of Secondary Metabolite, 2020

Free radicals are shown as the main reason of many chronic and degenerative diseases. Because of ... more Free radicals are shown as the main reason of many chronic and degenerative diseases. Because of the toxicity and undesirable effects of synthetic antioxidants, finding of new antioxidant natural agents is very important, especially edible plants. As an edible plant, valerian is used for various aims. Also, Valeriana species are known with their antioxidative compounds. In our previous study, some biological activities of Valeriana alliariifolia Adams roots extracts, collected in 2012, and the chemical compositions of active samples were determined. For this study, after 5 years collected plant materials were investigated again to determine and compare the antioxidant activities, the total phenolic contents and the chemical composition profile of the extracts from different plant-parts and to compare obtained results with the previous data. While RWI, RWM1 and RWM1residue were found most active by DPPH method and AHM1 by FRAP method, REM1 showed the highest activity by CUPRAC method. The high activity of AHM1 is parallel to its phenolic content. It can be thought that the difference between the results of our two studies, is due to the change of plant content from year to year and various environmental factors. This is important for achieving standardization in the production of its preparations.

International Journal of Secondary Metabolite, 2020

This study on Acer campestre L. subsp. campestre, used traditionally in treatments of various inf... more This study on Acer campestre L. subsp. campestre, used traditionally in treatments of various inflammation disorders, especially hemorrhoids and rheumatism, aimed to determine the total phenolic contents, the antioxidant and antimicrobial potentials, and the chemical composition profile of the extracts and subextracts from maple syrup and Acer campestre L. subsp. campestre leaves and twigs. The antioxidant activities of maple syrup extracts and subextracts were examined by 2,2-diphenyl-1-picryl-hydrazyl (DPPH), and the total phenolic contents of all samples were determined using the Folin-Ciocalteu reagent (FCR) method. Also, they were tested for their antimicrobial activities by microbroth dilution technique. Catechic tannin, saponins and flavonoids are determined in its leaves and twigs by preliminary qualitative phytochemical analysis. The alcoholic extracts obtained by 48-hour maceration (91.2%), ethyl acetate subextract of alcohol extracts obtained by 24-hour maceration (91.1%) and alcohol extracts obtained by 24-hour maceration (88.8%) exhibited higher radical scavenging activity than other samples, while the decoction and infusion had moderate activities. Beside of this, it was found that the syrup and its subextracts have more total phenolic contents than other extracts. The alcohol extracts obtained by 24-hour maceration and by 48-hour maceration exhibited higher activity against Candida albicans (78 μg/mL and 156 μg/mL, respectively). Only the alcohol extract obtained by 48-hour maceration and ethyl acetate subextract of syrup showed an activity against Escherichia coli, while all samples except butanol subextract of syrup have an antibacterial activity against Pseudomonas aeruginosa.

Istanbul Journal of Pharmacy, 2019

Marmara Pharmaceutical Journal, 2017

African journal of microbiology research

The antimicrobial activities of the different aerial and root extracts of Salvia cilicica were de... more The antimicrobial activities of the different aerial and root extracts of Salvia cilicica were determined aiming to evaluate whether, it can be used in phytotherapy as an antimicrobial agent. In this study, the antimicrobial activity of roots and aerial parts of Salvia cilicica extracts was evaluated using micro dilution and disc diffusion methods against Gram positive and Gram negative reference standard microorganisms and yeast Candida albicans. All of the extracts, with the exception of ethanol extract, showed antimicrobial activity by using MIC and MBC tests. The roots-petroleum ether and acetone extracts showed the highest antimicrobial activity (MIC’s ranges 20-313 μg/ml) against Gram positive bacteria. The root-acetone extract showed higher antimicrobial activity against Gram negative bacteria in comparison to other extracts. The most remarkable result of the antimicrobial activities is that, except for the ethanol extract, the Salvia cilicica had a good inhibitory effect (MI...

Zeitschrift für Naturforschung C, 2012

From the roots of Aconitum vulparia Rchb., collected in Prüm (Germany), a new norditerpenoid alka... more From the roots of Aconitum vulparia Rchb., collected in Prüm (Germany), a new norditerpenoid alkaloid, named alexhumboldtine, has been isolated along with the known norditerpenoid alkaloids lappaconitine, anthranoyllycoctonine, lycoctonine, puberaconitine, ajacine, and septentriodine. The structure of alexhumboldtine was established on the basis of 1H, 13C, DEPT, homonuclear 1H COSY, NOESY, HSQC, and HMBC NMR studies. From the aerial parts of the plant another norditerpenoid alkaloid, aconorine, has been isolated

Osmanlı Bilimi Araştırmaları, 2018

Mersin Universitesi Tıp Fakultesi Lokman Hekim Tıp Tarihi Ve Folklorik Tıp Dergisi, Apr 5, 2014

20th Annual Congress of International Drug Discovery Science and Technology - Japan 2024 (IDDST-Japan 2024), 2024

Insomnia is a condition defined by difficulties initiating and maintaining quality sleep despite ... more Insomnia is a condition defined by difficulties initiating and maintaining quality sleep despite plenty sleep chances, which disrupts daytime activities. Disrupting the sleep-wake cycle or having inadequate sleep may have negative effects on the central nervous system. Prolonged sleep deficit leads to impairment in metabolism, immune system and other regulatory systems. It is treated with the medicine like benzodiazepine receptor agonists, barbiturates, tricyclic antidepressants. Due to their adverse effects, herbal medicines, homeopathic remedies, and different nutritional supplements are also preferred. Studies, including those conducted by our research group, have has demonstrated that the rhizomes and roots of Valeriana species contain effective compounds on the central nervous system, such as valepotriates. Due to these compounds, Valeriana officinalis and Valeriana jatamansi can be used medicinally to treat anxiety, depression, and insomnia. Although they are preferred for therapeutic uses, recent research suggests that other Valeriana species may have comparable and promising potential. The purpose of this study is to assess the potential of other Valeriana species in the treatment of sleep disorders by evaluating research on their ethnobotanical use, chemical compositions, and bioactivities, as well as to determine whether they can be used safely against insomnia instead of medicinal species.

20th Annual Congress of International Drug Discovery Science and Technology - Japan 2024 (IDDST-Japan 2024), 2024

Neurodegeneration, slow and progressive dysfunction and loss of neurons and axons in the central ... more Neurodegeneration, slow and progressive dysfunction and loss of neurons and axons in the central nervous system, occurs as an undesirable consequence of neuroinflammation, although reactive oxygen species, i.e. free radicals, and inflammation caused by oxidative stress is a response to protect damaged tissues. This leads to acute and chronic neurodegenerative diseases like Alzheimer's disease and Parkinson's disease, as well as neurotropic viral infections, strokes, paraneoplastic disorders, traumatic brain damage and multiple sclerosis. Since the importance of oxidative stress and inflammation in the evolution of these diseases has been demonstrated, the number of studies looking for therapeutic treatments by discovering agents with strong antioxidant and anti-inflammatory potential is increasing by the day.

Fraxinus species contain secoiridoids, phenylethanoids and phenolic compounds, and have various activities such as anticancer, anti-inflammatory, antioxidant and neuroprotective. In addition to various investigations into their high antioxidant and anti-inflammatory effects, our research group discovered that Fraxinus ornus subsp. ornus has also good antioxidant, anti-inflammatory and neuroprotective activities. Although previous study has concentrated on specific species, the goal is to examine and reveal the potential of all investigated Fraxinus species in the treatment of neurodegenerative diseases, including Parkinson's and Alzheimer's.

Planta Medica, 2022

of human cancers. Previous studies have demonstrated that certain of these compounds inhibit cell... more of human cancers. Previous studies have demonstrated that certain of these compounds inhibit cell proliferation and induce apoptosis. However, there is no systematic study of all the compounds under the same conditions due to the difficulties related to their isolation in pure form. We have developed new methods for large-scale selective extraction of these compounds [1,2], making them affordable for detailed investigation. One step extraction of olive leaves using cold water and EtOAc permitted isolation of oleacein in 95 % purity without chromatography. Similarly, extraction of intact olive leaves with dichloromethane permitted isolation of pure oleomissional without chromatography. Alkaline treatment of oleomissional led quantitatively to oleuropein aglycon. Finally, water extraction of olive oil led to pure oleocanthal that was further oxidized to oleocanthalic acid. All the pure compounds as well as their combinations were assessed for their cytotoxic action on sixteen human cancer cell lines. EC 50 values were calculated for each isolated compound and their cytotoxic/anti-proliferative effect were estimated when different OOPs were combined. Finally, olive oil extracts of determined OOPsʼ content were tested. It was demonstrated that all OOPs impair cancer cell viability in a dose and time dependent manner in all cancer cells, while oleocanthal (EC 50 = 9 μM) was the most effective in all cancer cell lines. Most combinations of OOPs showed strong synergistic effect, whereas OOPsʼ extracts strongly impaired tumour cell viability even in the most resistant cancer cell lines. References [1] Melliou E, Magiatis P, Diamantakos P, Rigakou A. Method for obtaining of oleacein and oleomissional secoiridoids and method of producing pharmaceutical preparations thereof. WO2020165613 [2] Melliou E, Magiatis P, Diamantakos P, Rigakou A. Method for obtaining of oleocanthal secoiridoids and method for producing respective pharmaceutical preparations. WO2020165614