Adriana Martinez-Prado | ITD - Academia.edu (original) (raw)

Papers by Adriana Martinez-Prado

Mycobacterium vaccae JOB5 and Graphium sp. were studied to evaluate their ability to cometabolize... more Mycobacterium vaccae JOB5 and Graphium sp. were studied to evaluate their ability to cometabolize methyl tert-butyl ether (MTBE) and its metabolites after growth on two different alkanes, propane and iso-pentane. Both cultures were capable of cometabolizing MTBE and the metabolites, tert-butyl formate (TBF) and tert-butyl alcohol (TBA). MTBE, TBF, and TBA did not support growth of either microbe. Higher degradation rates were obtained in the bacterial system when the cultures were grown on iso-pentane. Nonlinear least squares regression and direct linear plot methods were used to estimate kinetic coefficients and provided comparable results. The enzymes from Mycobacterium vaccae JOB5 and Graphium sp. that promote the cometabolism of MTBE and its metabolites exhibited similar kinetics and substrate inhibition. The presence of the substrate decreased the degradation rate of MTBE and TBA suggesting competitive inhibition and preference for the substrate. Blockage experiment with acetyl...

Hidrobiológica: [revista del Departamento de Hidrobiología]

Aquatic and semi-aquatic plant species of three different water qualities were inventoried, two o... more Aquatic and semi-aquatic plant species of three different water qualities were inventoried, two of the El Tunal river and one of one of its tributaries, considering its content of dissolved oxygen, soluble phosphates, nitrate, ammonia, fecal coliforms, total suspended solids, and measurements of pH and electrical conductivity. A MANOVA/ANOVA demonstrated significant differences among parameters and sites. Twenty-eight species were identified; from them: Schoenoplectus americanus, S. tabernaemontani and Eleocharis densa were selected. All three were grown successfully under greenhouse conditions. Adaptation to local wastewater was evaluated using 5 micro-units: one control with wastewater (WW), another with gravel (G), and three sub-surface flow wetlands, one for each of the three selected plants, in duplicate. For ammonia and phosphate concentration, the systems with gravel removed 96 - 98%, and 99 - 100%, respectively. Fecal coliforms content was reduced about the same in all syste...

Los humedales artificiales son considerados como procesos físico-biológicos aplicados en la depur... more Los humedales artificiales son considerados como procesos físico-biológicos aplicados en la depuración de agua residual y dentro de las alternativas que existen al respecto, son los que menos gastos de operación requieren. Implementarlos, demanda el desarrollo de conocimientos básicos; entre estos están: determinar el listado de las plantas acuáticas nativas con potencial para ser usadas, conocer los materiales propios de la región que pueden ser aprovechados como soportes y sus efectos en la depuración y por último hay que establecer la hidráulica que debe ser usada en los sistemas. Este trabajo muestra el listado de plantas acuáticas de la región de Durango, Dgo. y su relación con calidad del agua; además de establecer el papel que juegan tres de las especies y el soporte utilizado en la remoción de fosfatos solubles, amoniaco y coliformes fecales de agua residual doméstica, en micro-humedales sub-superficiales.

Journal of Hazardous Materials, 2011

We previously isolated and identified Enterobacter sp. NKNU02 as a methyl tert-butyl ether (MTBE)... more We previously isolated and identified Enterobacter sp. NKNU02 as a methyl tert-butyl ether (MTBE)degrading bacterial strain from gasoline-contaminated water. In this study, tert-butyl alcohol, acetic acid, 2-propanol, and propenoic acid were detected using gas chromatography/mass spectrometry when MTBE was degraded by rest cells of Enterobacter sp. NKNU02 cells. We also found that biodegradation of MTBE was decreased, but not totally inhibited in mixtures of benzene, toluene, ethylbenzene and xylene. The effects of MTBE on the biology of Enterobacter sp. NKNU02 were elucidated using 2D proteomic analysis. The cytoplasmic proteins isolated from these MTBE-treated and -untreated cells were carried out for proteomic analysis. Results showed that there were 6 differential protein spots and 8 differential protein spots, respectively, as compared to their corresponding control (without MTBE addition), at the indicated incubation times when 40% and 60% of 100 mg/L of MTBE had been removed, Among these proteins, nine were successfully identified with matrix-assisted laser desorption ionization-time of flight-mass spectrometry. Proteins identified included extracellular solute-binding protein, periplasmic-binding protein ytfQ, cationic amino acid ABC transporter, isocitrate dehydrogenase, cysteine synthase A, alkyl hydroperoxide reductase (AhpC), transaldolase, and alcohol dehydrogenase. Based on these differential proteins, we discuss the bacterial responses to MTBE at the molecular level.

Hidrobiológica, 2009

Aquatic and semiaquatic plant species of three different water qualities were inventoried, two o... more Aquatic and semiaquatic plant species of three different water qualities were inventoried, two of the El Tunal river and one of one of its tributaries, considering its content of dissolved oxygen, soluble phosphates, nitrate, ammonia, fecal coliforms, total ...

uaemex.mx

Introduction. Methyl tert-butyl Ether (MTBE) is an additive that has been used to substitute lead... more Introduction. Methyl tert-butyl Ether (MTBE) is an additive that has been used to substitute lead tetraethyl (LTE) in reformulated gasoline. It has a high water solubility MTBE (hydrophilic characteristics) showing low affinity to partition with organic fraction in soil ...

Se realizó el inventario estatal de emisiones de gases de efecto invernadero para el estado de Du... more Se realizó el inventario estatal de emisiones de gases de efecto invernadero para el estado de Durango tomando como base el
año 2005 así como su tendencia para el período 2005-2008. Se consideraron únicamente al CO2, CH4 y N2O en el cálculo, por ser los tres GEI directos más importantes, y las cifras totales están expresadas en Gigagramos de CO2 equivalente. Los resultados promedio anuales obtenidos para cada una de las categorías fueron: Energía (11,400.54), Agricultura (2,664.90), Procesos Industriales y Solventes (874.80) y Residuos (693.78); observándose una tendencia creciente del 2005 al 2008 equivalente al 28.25%. En el caso de la categoría de Uso de Suelo, Cambio de Uso de Suelo y Silvicultura se realizó un balance de captura neta (- 4,007.01) y emisión total (4,298.18) dando como balance total 272.17 Gg CO2 eq. Las emisiones totales para el 2005 y 2008 fueron 13,474.04 y 17,281.12, respectivamente; con un promedio para el periodo 2005-2008 de 15,906.19 Gg CO2 eq. También se estimaron las emisiones de los gases indirectos: NOx, CO, COVNM y SO2, expresados
en Gigagramos; siendo el CO el más abundante. Con base en la identificación de las fuentes de emisión en cada categoría se propusieron las medidas de mitigación correspondientes.

This study was conducted with a fungal strain isolated from a mining soil contaminated with total... more This study was conducted with a fungal strain isolated from a mining soil contaminated with total petroleum hydrocarbons (TPH) and properly identified by polymerase chain reaction (PCR) technique as Aspergillus terreus KP862582. The biodegradation potential of this pure culture was evaluated at laboratory scale; a wide diesel concentration range, from 10,000 to 50,000 mg diesel/kg soil (ppm), was tested using sterile soil microcosm over a 90-day period. Aerobic biodegradation of diesel by Aspergillus terreus KP862582 was significantly greater (p < 0:05) for 10,000, 20,000, and 30,000 ppm, with rate constant values of 0.025, 0.023, and 0.012 1/day, respectively. Cell viability at these concentrations was favored because it showed a significant increase during the first period of biodegradation (0-30 days), from this time onwards eciency removal and cell viability decreased considerably. This pattern was observed as concentration of diesel increased, resulting in a much lower biodegradation rate for 40,000 ppm (0.005 1/day) and 50,000 ppm (0.002 1/day). Based on the results of this study it is concluded that the strain of Aspergillus terreus KP862582 can be used in the bioremediation
of soils contaminated with petroleum hydrocarbons at concentrations of 10,000 and 20,000 ppm, and comply with the MPL established by the Mexican regulation.

M.G. Vicencio-de la Rosa, R. Valencia-Vázquez, M.E. Pérez-López, M.A. Martínez-Prado and E.S. Ra... more M.G. Vicencio-de la Rosa, R. Valencia-Vázquez, M.E. Pérez-López, M.A. Martínez-Prado and E.S.
Ramirez-Ramirez. Revista MExicana de Ingeniería Química: Vol. 14, No. 1 (2015) 61-71 pp. Publicado en Abril del 2015.

A wastewater plant of a dairy industry generates 1.6 tons/month of settleable solids and 650 kg/week of floatable solids,which if not adequately disposed off, can cause serious soil contamination and vector attraction. A composting process for stabilizing residues with high fat content that generate unpleasant odors is presented. Laboratory experiments were carried out for determining the appropriate process parameters, and scaled-up for the in situ process. Laboratory tests with cattle manure showed a reduction in the production of malodorous gases in three weeks. The resulting compost complied with national and international standards, and can be used in 1:3 compost:soil ratio for soil improvement. The waste stabilized in situ complied with most parameters established in the Mexican regulation (NOM-FF-109-SCFI-2007), excepting pH (9.6) and electrical conductivity (5 dS/m).

Roberto Valencia-Vázquez, María Elena Pérez-López, María Guadalupe Vicencio-de-la-Rosa, María Adr... more Roberto Valencia-Vázquez, María Elena Pérez-López, María Guadalupe Vicencio-de-la-Rosa, María Adriana Martínez-Prado & Rubén Rubio-Hernández. Waste Management & Research
Publicado: Septiembre 2014; Vol. 32, 9: 848-856 pp.
As society evolves its welfare level increases, and as a consequence the amount of municipal solid waste increases, imposing great challenges to municipal authorities. In developed countries, municipalities have established integrated management schemes to handle, treat, and dispose of municipal solid waste in an economical and environmentally sound manner. Municipalities of developing and transition countries are not exempted from the challenges involving municipal solid waste handling, but their task is not easy to accomplish since they face budget deficits, lack of knowledge, and deficiencies in infrastructure and equipment. In the northern territory of Mexico, the municipality of Durango is facing the challenge of increased volumes of waste with a lack of adequate facilities and infrastructure. This article analyses the evolution of the municipal solid waste management of Durango city, which includes actions such as proper facilities construction, equipment acquisition, and the implementation of social programmes. The World Bank, offering courses to municipal managers on landfill operation and waste management, promoted the process of knowledge and technology transfer. Thereafter, municipal authorities attended regional and some international workshops on waste management. In addition they followed suggestions of international contractors and equipment dealers with the intention to improve the situation of the waste management of the city. After a 15-year period, transfer of knowledge and technology resulted in a modern municipal solid waste management system in Durango municipality. The actual system did not reach the standard levels of an integrated waste management system, nevertheless, a functional evaluation shows clear indications that municipality actions have put them on the right pathway.

ISOLATION AND CHARACTERIZATION OF A NOVEL STRAIN, Bacillus sp KJ629314, WITH A HIGH POTENTIAL TO AEROBICALLY DEGRADE DIESEL , May 1, 2014

In this research, a diesel-degrading bacterium (strain KJ629314) was isolated from a mining soil ... more In this research, a diesel-degrading bacterium (strain KJ629314) was isolated from a mining soil contaminated with total petroleum hydrocarbons (TPH) and properly characterized using the polymerase chain reaction (PCR) molecular technique. The 16s rDNA sequence analysis allowed to identify KJ629314 as a strain of Bacillus sp. Experimental phase was conducted to assess the aerobic biodegradation of diesel; to determine the removal efficiency and the corresponding microbial growth; diesel was used as a substrate - electron donor - carbon source; and oxygen (via aeration) as the electron acceptor. Tests were conducted in microcosms with sterile sand with nutrients according to the N:P ratio (15:1) at different diesel concentrations (10,000; 20,000; 30,000; 40,000 and 50,000 mg/kg soil). Results showed that the strain of Bacillus sp KJ629314 has a high potential in the biodegradation of diesel at the evaluated concentrations, and it was demonstrated that the removal efficiency was greater at low concentrations of diesel obtaining higher values for the microbial growth and diesel biodegradation rate constants. These promising results support the fact that Bacillus sp KJ629314 may be used as a novel biological resource to develop a bioprocess for the bioremediation of diesel-contaminated soil.

Contaminated soil with Total petroleum hydrocarbons (TPH) was subjected to treatment in an experi... more Contaminated soil with Total petroleum hydrocarbons (TPH) was subjected to treatment in an experimental prototype with effluent recirculation. A 2k experimental design was used, with two factors (electric field and microorganisms’ growth) and two levels (presence and absence of the factors), resulting in 4 treatments (triplicate) designated as: leaching, bioremediation, electroremediation, and electrobioremediation. Total petroleum hydrocarbons attached to the soil surface showed a higher migration to the aqueous phase due to the application of low intensity current. Growth of native microorganisms present in the soil was stimulated with the addition of micro and macronutrients; TPH were degraded used as carbon source and electron donor, using oxygen (aeration) as electron acceptor. There were statistical significant differences between treatments supporting EBR as the best alternative. Results showed 24.5 ± 3.1% of TPH removal from the soil into the aqueous phase for lixiviation and 32.1 ± 1.4% for bioremediation, both at 360 hours. For electroremediation a 54.8 ± 2.2% removal (360 h) and 68% (700 h) and 68.6 ± 3.8% (360 h) and 89% (700 h) for electrobioremediation. Remediated soil in electrobioremediation reached 2,596 mg TPH/kg soil, such concentration is lower than the maximum permissible limit (MPL) set by Mexican regulation

Fluorine (F) and arsenic (As) are inorganic elements present in the subsurface depending on the g... more Fluorine (F) and arsenic (As) are inorganic elements present in the subsurface depending on the geology of the region. These compounds are found in high concentrations in the underground strata of Guadiana Valley of Durango affecting water quality for human consumption (NOM-127-SSA-1994). In the present research the main objective was to assess the behavior in time and space of fluoride (F − ) and arsenic concentration, from 1996 to date, in the groundwater of the city of Durango and some wells of the rural area as a reference. The highest concentration of arsenic was found in a rural well, Colonia Hidalgo (0.149 mg/L or ppm), 6 times the maximum permissible limit (MPL); within the city well 54 located in the western sector had the highest value (0.076 ppm), 3 times the MPL, 67% of the wells in the city and 60% of the Guadiana Valley had levels that exceeded the MPL (0.025 ppm), the concentration in the city ranged from 0.009 to 0.149 ppm and from 0.08 to 0.15 ppm for the rural zone. With respect to F − , the highest value was also found in the Colonia Hidalgo well (17.8 mg/L), 12 times the MPL; within the city the highest value was recorded in well 16 in the eastern sector with 7.6 ppm (5 times the MPL) 97% of the wells in the city and 100% of the wells in the Guadiana Valley rural area had concentrations greater than MPL (1.5 ppm), the concentration in the city ranged from 1.1 to 7.6 ppm, while in the Guadiana Valley from 1.7 to 17.8 ppm. Statistical analysis showed no significant difference for fluoride concentration over time (1996)(1997)(1998)(1999)(2000)(2001)(2002)(2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010)(2011)(2012)(2013); whereas the concentration of arsenic decreased, probably due to the degree of precision required for such small concentrations in groundwater and different analysts ran the samples.

Arsenic and fluoride are elements known to cause human health problems and it has been documented... more Arsenic and fluoride are elements known to cause human health problems and it has been documented that both elements are found in high concentrations in the Guadiana Valley aquifer, in the state of Durango, Mexico. Since underground water is the source for potable water bottling companies commercialized in Durango City; such high concentrations reduced the quality of bottled water for human consumption according to NOM-041-SSA1-1993. Legislation establishes a maximum permissible limit (MPL) of 0.7 mg/L for fluoride and 0.025 mg/L for arsenic. In this research the main objective was to evaluate the quality of bottled water expended in Durango City with respect to the well from which water is extracted. Findings showed that the highest fluoride concentration was 5.86 mg/L (8.4 times MPL), with 100% of sampled brands exceeding the MPL (range: 1.09 to 5.86 mg/L). On the other hand, for arsenic, the highest concentration was 0.076 mg/L (threefold), with 38% exceeding the MPL (range: 0.001 to 0.076 ppm). Statistical analysis showed significant differences only for fluoride, according to Fisher LSD (Least Significant Difference) test, with an F value of 14.5 at a p value of 0.0005. According to the comparison between the quantified concentrations in bottled water and groundwater, it was found that groundwater was subjected to treatment; however, although a significant decrease in fluoride and arsenic concentration was observed, the removal processes used were not efficient to meet set standards.

La modelación de impactos locales, caracterizados por deterioro de los recursos naturales -especi... more La modelación de impactos locales, caracterizados por deterioro de los recursos naturales -especialmente agua y suelo-ante los efectos globales del cambio climático, se ha convertido en una poderosa herramienta en la búsqueda de medidas de mitigación y adaptación. Los objetivos de la presente investigación fueron: 1) evaluar mediante procesos de modelación el impacto potencial del cambio climático para el periodo 2010-2039 y 2) advertir sobre riesgos futuros a partir de la identificación de forzantes radiativos locales o áreas críticas, considerando el índice de aridez (IA) y erosión laminar del suelo causada por el viento (ELV) como dos indicadores de calidad ambiental. Se usaron técnicas de evaluación de los recursos naturales empleadas por el Instituto Nacional de Ecología y Cambio Climático (INECC) para los estudios de ordenamiento ecológico territorial. Los insumos empleados comprenden información climática actual y futura, cubiertas de suelos y propiedades edáficas asociadas al municipio de Gómez Palacio, Durango, México (25.886º N y 103.476º W). Los cálculos realizados a partir de las anomalías para los promedios anuales de precipitación y temperatura indican que el territorio municipal en el escenario A2 podría tener un impacto promedio de 63% causado por la ELV, en tanto que el IA probablemente cambie su promedio histórico de 9.3 a 8.7; se estima que el impacto promedio sobre este índice en el futuro será de 0.53 ± 0.2.