"ISOLATION AND CHARACTERIZATION OF A NOVEL STRAIN, Bacillus sp KJ629314, WITH A HIGH POTENTIAL TO AEROBICALLY DEGRADE DIESEL " (original) (raw)
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2020
In Mexico, one of the principal natural resources is oil, however, the activity related to it has generated hydrocarbon spills on agricultural soils. The aim of this study was to evaluate the biodegradability of diesel by means of indigenous bacteria isolated from agricultural soil contaminated with 68 900 mg kg<sup>-1</sup> diesel. We examined indigenous bacterial strains in agricultural soils contaminated with diesel from Acatzingo, Puebla, Mexico. We performed a physicochemical soil characterization, and a bacterial population quantification favoring sporulated bacteria of the genera <i>Bacillus</i> and <i>Paenibacillus</i> taken from the study site. Six bacterial strains were isolated. The identification was made based on the 16S rRNA gene and API systems. The tolerance and biodegradation capacity in diesel were determined at 4 000 to 24 000 mg L<sup>-1</sup> of diesel. Residual concentrations of diesel were determined by GC-FID. S...
Isolation and characterization of a novel native Bacillus strain capable of degrading diesel fuel
International Journal of Environmental Science & Technology, 2009
The ability of native bacteria to utilize diesel fuel as the sole carbon and energy source was investigated in this research. Ten bacterial strains were isolated from the oil refinery field in Tehran, Iran. Two biodegradation experiments were performed in low and high (500 and 10000 ppm, respectively) concentration of diesel fuel for 15 days. Only two isolates were able to efficiently degrade the petroleum hydrocarbons in the first test and degraded 86.67 % and, 80.60 % of diesel fuel, respectively. The secondary experiment was performed to investigate the toxicity effect of diesel fuel at high concentration (10000 ppm). Only one strain was capable to degrade 85.20 % of diesel fuel at the same time (15 days). Phenotype and phylogeny analysis of this strain was characterized and identified as dieseldegrading bacteria, based on gram staining, biochemical tests, 16S rRNA gene sequence analysis. These results indicate that this new strain was Bacillus sp. and could be considered as Bacillus Cereus with 98 % 16 S rRNA gene sequence similarity. The results indicate that native strains have great potential for in situ remediation of diesel-contaminated soils in oil refinery sites.
IRJMETS Publication, 2022
Environmental pollution with petroleum has been recognized amongst the serious threating problems globally. Some of the most existing petroleum hydrocarbons pollutants include diesel, gasoline and fuel oils. In the recent times, bioremediation/biodegradation techniques are used to remove environmental pollutants. A list of microorganisms have the ability to use petroleum hydrocarbons as the sole source of carbon for energy in order to carry out their metabolic activities. It involves the use of microbes to purify and humiliate environmental contaminants. The present study is carried out during 2019-20 by conducting isolation, identification and optimization of diesel degrading microbes from diesel oil polluted in selected sites of Peshawar, Pakistan. Bacteria like Acinetobacter baumani, Acinetobacter pitti, Acinetobacter calcoactius, and Pseudomanas auroginosa were isolated on the basis of morphological and physiological characteristics. Further the optimization was carried out for various culture parameters i.e., Hydrocarbon concentration and pH. The present study suggests that Acinetobacter pitti has the degrading ability at pH 7 when hydrocarbon concentration is kept at 2%.
Exploiting bacterial isolates for diesel degrading potential under in vitro conditions
Plant Science Today, 2022
Hydrocarbon contaminated oil-spilled areas and oil-products have caused serious harm with increasing attention for development, implementation and removal of these contaminants. Bacterial diversity on succession at the petroleum hydrocarbon contaminated environment can give answer the problem. Such lands have serious problems as totally barren or with rare plantation. Bacteria can thereby be exploited for the mitigation of hydrocarbon to enhance the nutrient availability for vegetation. Present study involves collection of soil samples heavily contaminated with hydrocarbon from Bagru (Rajasthan). Samples were analysed by solid liquid extraction method followed by FTIR (Fourier Transform Infrared) and HPLC (High Performance Liquid Chromatography) analysis. During microbiological analysis hydrocarbon degrading bacteria were screened. FTIR spectral analysis indicated the presence of the functional group's alkanes and aromatic ringed compounds; 43% to 69% hydrocarbon content recorded by HPLC analysis of all the soil samples respectively. From the soil samples six gram-positive and four gram-negative bacterial isolates were explored possessing hydrocarbon degrading capacities in the range 47.04-87.31% and 10.12-95.24% respectively. Growth kinetic studies revealed the degradation up to 1000 ppm diesel in 3 days under in vitro conditions. These bacteria can further be exploited for diesel degradation and will certainly propose a possible solution to the prevailing issue for its biodegradation in ex-situ conditions after up scaling.
International journal of phytoremediation, 2017
Bioremediation of polluted soils is a promising technique with low environmental impact, which uses soil organisms to degrade soil contaminants. In this study, 19 bacterial strains isolated from a diesel-contaminated soil were screened for diesel-degrading potential, biosurfactant production, and biofilm formation abilities, all desirable characteristics when selecting strains for re-inoculation into hydrocarbon-contaminated soils. Diesel degradation rates were determined in vitro in minimal medium with diesel as the sole carbon source. The capacity to degrade diesel range organics (DRO) of strains SPG23 (Arthobacter sp.) and PF1 (Acinetobacter oleivorans) reached 17-26% of total DRO after 10 days, and 90% for strain GK2 (Acinetobacter calcoaceticus). The amount and rate of alkane degradation decreased significantly with increasing carbon number for SPG23 and PF1. Strain GK2, which produced biosurfactants and biofilms, exhibited a greater extent, and faster rate of alkane degradatio...
Isolation of bacteria from diesel contaminated soil for diesel remediation
Journal of Bio-Science, 2019
This study is aimed at isolating bacterial species that inhabit diesel contaminated soil and also screened these isolates for the ability to be used for remediating diesel contaminated environment using their potential to degrade diesel as carbon and energy source. Top soil sample was collected from an ancient diesel-powered generator house in Minna, Nigeria, in a sterilized plastic container while diesel oil was obtained from local petrol bunk. Four bacterial isolates were isolated from the diesel contaminated soil sample and were screened for their ability to degrade diesel using mineral salt medium (MSM). The isolates with highest biodegradation potential were identified as Bacillus subtilis and Bacillus cereus. The optimum pH (5, 6, 7 and 8) and hydrocarbon concentration (1%, 2%, 5% and 10%) of the isolate was determined by spectrophotometry and the result revealed that the optimum pH for biodegradation of diesel by Bacillus subtilis and Bacillus cereus, was 7 (1.170) and 8 (1.7...
Isolation and Characterization of Diesel Degrading Bacteria from Petroleum Oil Contaminated Soil
Bacterial Empire
Petroleum products are used for energy production and an essential part of our day-to-day lives especially in vehicles, ships, and industries. Accidental leakages occur easily and wastage petroleum is also discarded in the environment without any further processing causing environmental pollution. Diesel contributea big part topetroleum pollution. The current study was aimed to identify diesel degrading bacteria and determine some conditions to evaluate their best degradation capability. We identified Aeromonas spp., Bacillus spp., and Enterobacter spp. from diesel contaminated soil and found that Aeromonas spp. and Bacillus spp. grow best with 10% to 15% diesel whereas Enterobacter spp. can grow quite well with 20% diesel concentration at a higher temperature (40oC) than the previous two bacteria. Aeromonas spp. worked well at low pH (pH 4 to pH 6) whereas Bacillus spp. and Enterobacter spp. worked best at higher pH (pH 10).
Isolation and Identification of Effective Hydrocarbon Degrading Bacteria from Diesel Polluted Soil
IOSR Journal of Biotechnology and Biochemistry, 2017
Hydrocarbon pollution is due to accidental leakages, spills or by transportation. It might be carcinogenic to human, animal and plant health. Bioremediation is the best remedy to remove the pollutants from the environment. Diesel degrading microbes are widely distributed in our environment. The microbes may not be present in sufficient amount to achieve the degradation process in successfully. Inoculation of efficient hydrocarbon degrading bacterial strains in polluted area will enhance the capability of degradation. The present study was aimed to be isolating the effective bacterial strains from the polluted area to enhance the degradation. Totally fifteen isolates were identified and biochemically characterized. Among these two of them were effective degrader under 4% diesel. Pseudomonas putida and Micrococcus luteus are the bacterial strains were able to degrade the contaminants in large amounts. In this study we report the bacterial strains capable of degrading the hydrocarbons efficiently. Degradation studies to be carried out with different isolates at different concentration of diesel to find out most potent hydrocarbon degrading strains, which can be used for any bioaugmentation studies during bioremediation
Journal of Biochemistry, Microbiology and Biotechnology
The high rise in the exploration and usage of petroleum products have led to widespread contamination in the environment. Thence, the continuous search for microorganisms with the potential to mineralize these pollutants is necessary. This study was conducted to isolate, identify and determine the diesel degradation potential of bacteria from oil-contaminated soil collected from three filling stations in Azare, Katagum LGA, Bauchi State, Nigeria. The diesel degrading bacteria were identified using standard protocols. The isolates were screened spectrophotometrically for their potential to utilize 1% diesel (v/v) as their sole carbon and energy source and the best candidate was used for determining the effect of diesel concentration on its biodegradation. The results revealed the presence of three bacterial species including Pseudomonas aeruginosa, Pseudomonas putida and Bacillus subtilis. P. Putida showed the highest diesel degradation at 120 h with an absorbance of 2.27±0.03 at 600...
Studies on the Bioremediation Potential of Bacteria Isolated from Diesel-contaminated Soils in Kano
Journal of Biochemistry, Microbiology and Biotechnology
Bioremediation is pollution control technology that uses microorganism to clean up contaminated environment. This study was aimed at assessing the bioremediation potential of bacteria isolated from diesel-contaminated soils and screen them for the ability to remediate diesel contaminated environment using their potential to degrade diesel as carbon and energy source. Diesel-contaminated soil samples were collected using standard method. Bacteria were isolated, characterized and identified using standard microbiological procedures. The identified bacteria species were subsequently screened for diesel biodegradation potential in Bushnell Haas Media (BHM). Optimum conditions (pH, temperature, and diesel concentrations) for biodegradation were determined. The results showed that Bacillus subtilis and Bacillus megaterium were the most potent species identified. Bacillus subtilis and Bacillus megaterium were observed to utilize diesel as the sole carbon source in which they degrade 79% an...