Md Irshad | Jamia Millia Islamia (original) (raw)

Papers by Md Irshad

Intrinsic viscosities [η] for 0.2, 0.4, 0.6, and 0.8 mg% aqueous of each Bovine serum albumin (B... more Intrinsic viscosities [η] for 0.2, 0.4, 0.6, and 0.8 mg% aqueous of each Bovine serum albumin
(BSA) and egg albumin (EA) solutions under zero, 0.5 and 0.7 magnetic flux at 298.15 K and
1 atmosphere are reported. The [η] data for BSA are higher than EA and values of both
increase with magnetic flux strengthening molecular interactions.

Doses of 0.0150 weber m− 2 magnetic flux for 30 minutes are given to four series of biomolecules... more Doses of 0.0150 weber m− 2 magnetic flux for 30 minutes are given to four series of
biomolecules in aqueous medium, densities (ρ), viscosities (η), and surface tension (γ) were
measured at 304.15 K. The vitamins and carbohydrates decrease densities and viscosities
and the difference of density from magnet to aqueous solution in order of dextrose (−
0.00025 kg m− 3)> fructose (− 0.00021 kg m− 3)= B6 (− 0.00021 kg m− 3)> starch (−
0.00018 kg m− 3)> B1 (− 0.0018 kg m− 3), while viscosity in order of starch (− 0.0044 kg m ...

Cassia fistula oil was investigated for antifungal activities against standard and clinical isola... more Cassia fistula oil was investigated for antifungal activities against standard and clinical isolates of Candida species. Gas chromatography coupled with mass
spectrometric (GC-MS) analysis of C. fistula oil revealed the presence of antimicrobial compounds like β-sitosterol, stigmasterol, ergosterol, betulinic acid,
lupeol, fucosterol, α-amyrin and friedelin. The minimum inhibitory concentration (MIC) of the pulp and seed oils ranged between 250-300 and 350-500 μg/mL
respectively. Both oils also inhibited by ≥63.8% ergosterol bio-synthesis in Candida cell wall {fluconazole (standard) ≥89.1%)}. The MICs were significantly
correlated with the ergosterol content decrease in the cell wall (Student’s t test p ≤ 0.005). We can, therefore, conclude that active compounds are present in
Cassia fistula oil that primarily target ergosterol biosynthesis in Candida cell wall.

Cassia fistula Linn. (Fabaceae) fruit has been used in folk medicine and reported for various ph... more Cassia fistula Linn. (Fabaceae) fruit has been used in folk medicine and reported for various pharmacological properties. We evaluated the anticancer activity of C. fistula fruit extracts [ethyl acetate extract of pulp (EEP), n-butanol extract of pulp (BEP), ethyl acetate extract of seeds (EES), n-butanol extract of seeds (BES)] against human cervical cancer (SiHa) and breast cancer (MCF-7) cell lines. Phytochemicals analysis revealed that the EEP and EES contained anticancer compounds like 2(3H)-furanone, rhein, thymoland oleic acid. BES contained inositol and palmitic acid, whereas BEP contained inositol and 2-pyrrolidone. IC50 of EEP, EES, BEP and BES against SiHa cells were 415.5±0.19, 435.7±0.11, 535.3±0.32 and 580.2±0.41 μg/mL, whereas against MCF-7 cells were 422.2±0.32, 51.4±0.27, 564.5±0.21 and 575.6±0.47 μg/mL respectively (p < 0.01). At IC50, EEP, EES, BEP and BES treated MCF-7 cells formed colonies of 17.5, 16.3, 22.5 and 18.8 % whereas treated SiHa cells formed colonies of 11.5, 12.8, 19.2 and 16.7 % (p < 0.03). Seeds and pulp treated both cell lines showed up-regulation of p53 and Bax genes, down-regulation of Bcl-2 gene and increased
caspase-3, 7 & 10 and -9 enzymes activities. Also, the fragmented genomic DNA in pulp and seeds extracts treated SiHa and MCF-7 cells showed sign of apoptosis.We concluded that the fruit pulp and seeds extracts inhibited MCF-7 and SiHa cells growth and induced cells death by modulating the expression of apoptosisregulatory genes and caspase enzymes.

Laginaria siceraria have also shown a significant correlation (P < 0.05).

The anti-inflammatory activities of extract of Calotropis gigantea R.Br. and Tridax procumbens Li... more The anti-inflammatory activities of extract of Calotropis gigantea R.Br. and Tridax procumbens Linn., were assessed on carrageenin-induced paw edema along with standard drug, Ibuprofen. The Ibuprofen significantly reduced paw edema at the dose of 200mg/Kg bw orally. The oral administration equi-effective dose (ED 50 ) of C. gigantea (600mg/Kg bw) and T. procumbens (400 mg/Kg bw) individually revealed about 20-35% more activity than the one rendered by administration of 50mg/Kg bw of Ibuprofen. The effect of C. gigantea and T. procumbens along with various dose regimen of Ibuprofen showed greater anti-inflammatory activities than the Ibuprofen alone.

Antioxidant potential of various extracts of Cassia fistula was determined by the DPPH, FRAP, Fe ... more Antioxidant potential of various extracts of Cassia fistula was determined by the DPPH, FRAP, Fe 3+ reducing power, and hydrogen peroxide scavenging assay. Methanolic extracts of Cassia fistula showed the highest amount of phenolic and flavonoid content and reducing capacity, whereas hexane extracts exhibited the lowest level of reducing capacity. The order of antioxidant activity in Cassia fistula extracts displayed from higher to lower level as methanolic extracts of pulp, methanolic extracts of seed, hexane extracts of pulp, and hexane extracts of seed. The antioxidant potential of Cassia fistula extracts significantly correlated (P < 0.02) with the phenolic content of the methanolic extracts. Ascorbic acid taken as control showed highest antioxidant power in the present study.

Cassia fistula Linn, a semi-wild Indian labrum has been used in the treatment of various diseases... more Cassia fistula Linn, a semi-wild Indian labrum has been used in the treatment of various diseases in different parts of the world since time immemorial. In Indian literature there has been multiple descriptions regarding its usefulness in the treatment of various diseases. Some authors have reported its antibacterial, antioxidant, hepatoprotective and hypoglycemic potentials. Many other authors have indicated towards the free radical scavenging and antioxidant potential of C. fistula both in vitro as well as in vivo. Despite this valuable available information on this plant there appears a vast virgin area of research on this plant to find out the real natural treasure. The antioxidant and hepatoprotective nature of Cassia fistula seems to depend on the total phenolic, proanthocyanidin and flavanoid contents of this labrum. This paper reviews all the experimental studies so far performed on C. fistula. This is likely to have important implications on designing any future research on this plant for their potential utility in herbal drug system or in nutritional supplements.

Laginaria siceraria have also shown a significant correlation (P < 0.05).