An approach to rapid protein crystallization using nanodroplets (original) (raw)
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B. D. Santarsiero, D. T. Yegian, C. C. Lee, G. Spraggon, J. Gu, D. Scheibe, D. C. Uber, E. W. Cornell, R. A. Nordmeyer, W. F. Kolbe, J. Jin, A. L. Jones, J. M. Jaklevic, P. G. Schultz and R. C. Stevens
An approach that enables up to a two order of magnitude reduction in the amount of protein required and a tenfold reduction in the amount of time required for vapor-diffusion protein crystallization is reported. A prototype high-throughput automated system was used for the production of diffraction-quality crystals for a variety of proteins from a screen of 480 conditions using drop volumes as small as 20 nL. This approach results in a significant reduction in the time and cost of protein structure determination, and allows for larger and more efficient screens of crystallization parameter space. The ability to produce diffraction-quality crystals rapidly with minimal quantities of protein enables high-throughput efforts in structural genomics and structure-based drug discovery.
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