ESRA NURTEN YER | Kastamonu University (original) (raw)

Papers by ESRA NURTEN YER

Research paper thumbnail of Genome-wide identification and comparative expression analysis of LEA genes in watermelon and melon genomes

Physiology and Molecular Biology of Plants, 2017

Research paper thumbnail of Türkiye Orman Fidanlıklarında Yetiştirilen Süs Bitkilerinin Üretim Teknikleri Esra Nurten Yer*, Sezgin Ayan Kastamonu Üniversitesi, Orman Fakültesi Orman Mühendisliği Bölümü, 37200 Kastamonu * esranurtenyer@gmail.com

Süs bitkileri sektörü ekonomik olarak son yıllarda büyük ilerleme göstermiĢtir. Bu ilerlemede kon... more Süs bitkileri sektörü ekonomik olarak son yıllarda büyük ilerleme göstermiĢtir. Bu ilerlemede konu ile ilgili yapılan bilimsel toplantıların, sempozyum ve kongrelerin katkısı önemlidir. Bu tip organizasyonlarda; araĢtırmacılar, öğretim üyeleri, özel sektör ve sivil toplum kuruluĢları süs bitkileri sektöründeki sorunları ve çözüm önerilerini, bilimsel ve teknolojik geliĢmeleri birlikte tartıĢıp değerlendirme imkânı bulmaktadır. Bu değerlendirmelerden elde edilen sonuçlar hem Ar-Ge faaliyetlerinin etkinliğinin artması hem de sektörün geliĢmesi açısından büyük önem arz etmektedir.

Research paper thumbnail of Identification and expression analysis of LEA gene family members in cucumber genome

Plant Growth Regulation, 2016

LEA (late embryogenesis abundant) proteins are firstly discovered in seeds and then identified in... more LEA (late embryogenesis abundant) proteins are firstly discovered in seeds and then identified in vegetative tissues of different plant species. They are mainly regulated under abiotic stress conditions. Although genome wide studies of different gene family members have been performed in cucumber, there is no such a study for LEA genes. We have identified 79 LEA genes in the cucumber genome. Based on phylogenetic analysis, CsLEA genes could be classified into seven groups in which structural motifs are relatively conserved. Tandem duplications play an important role in cucumber genome for LEA gene expansion. Orthologous and chromosomal relationships of CsLEA genes were observed based on comparative mapping analysis with other species. The in silico micro-RNA (miRNA) target analyses indicated that 37 CsLEA genes were targeted by different miRNAs, especially mir854 and mir414 are the most abundant identified ones. Public available RNA-seq data were analyzed for expression analysis of CsLEA genes in different tissues of cucumber. According to genome-wide expression analysis, nine CsLEA genes showed higher expression profiles in all tissues. The expression profiles of ten CsLEA genes in the root and leaf tissues of drought-stressed cucumber were examined using qRT-PCR. Among them, CsLEA-54 induced after stress application in leaf and root tissues and might provide adaptation to drought stress for cucumber. CsLEA-09, CsLEA-32 and CsLEA-57 genes responded to drought after 3 h later and might be considered as early response genes to water limitation. This research could help us to improve understanding of contribution of CsLEAs to drought tolerance in cucumber.

Research paper thumbnail of Drought-Responsive Hsp70 Gene Analysis in Populus at Genome-Wide Level

Plant Molecular Biology Reporter, 2015

The heat shock protein 70 (Hsp70) family members are known as molecular chaperones. They play a c... more The heat shock protein 70 (Hsp70) family members are known as molecular chaperones. They play a crucial role in protecting plant cells and tissues from thermal or abiotic stress through protein folding and in assembly, stabilization, activation, and degradation processes. Although many studies have been performed to identify molecular functions of individual family members, there is a limited study on genome-wide identification and characterizations of Hsps in the Populus model tree genus. We have identified 34 poplar Hsp70 genes, which were phylogenetically clustered into three major groups. Gene structure and motif composition are relatively conserved in each group. Mainly tandem and infrequently segmental duplications have a significant role in poplar Hsp70 gene expansion. The in silico microRNA (miRNA) and target transcript analyses identified that a total of 19 PtHsp70 genes were targeted by 27 plant miRNAs. PtHSP70-14 and PtHSP70-33 are the most targeted by miR390 and miR414 family members, respectively. For determination of drought response to Hsp70 genes, publicly available RNA-seq data were analyzed. Poplar Hsp70s are differentially expressed upon exposure to different drought stress conditions. Expression analysis of PtHsp70 genes was also examined under drought stress in drought-sensitive and drought-resistant Populus clones with quantitative real-time PCR (qRT-PCR). PtHsp70-16 and PtHsp70-26 genes might provide adaptation to drought stress for both clones. Because of high expression responses to drought in only resistant Populus clone, PtHsp70-25 and PtHsp70-33 genes might be used for determination of drought-tolerant clones for molecular breeding studies. This research provides a fundamental clue for contribution of PtHsp70s to drought tolerance in poplar.

Research paper thumbnail of The occurrence of heart rot on Abies nordmanniana subsp. bornmülleriana Mattf. trees in relation to altitude

Kastamonu University Journal of Forestry Faculty, 2012

Fir is the taxon subjected to the selection systems in Turkish forestry. In selection forests, ta... more Fir is the taxon subjected to the selection systems in Turkish forestry. In selection forests, target diameter is the main criterion for planning and management. In this study the aim is to determine the formation and level of the heart rot and target diameter depending on aspect and altitude for Abies nordmanniana subsp. bornmülleriana Mattf. For this purpose, the fir trees with maximum diameters were cut and the situation of heart rot at various altitude levels (900-1,200 m, 1,200-1,500 m, and 1,500-1,800 m) and aspects was determined. The formation of heart rot starts at low altitudes and this defect was found nearly on all fir trees of target diameter of 60 cm which is commonly used in practical Turkish forestry. Target diameter of 60 cm was considered as usable at the altitude of 1,200 m and even higher, at 1,500 m and even more, the usable target diameter is more than 60 cm, as heart rot was not found in the sample trees with target diameters about 70-80 cm. In addition to thi...

Research paper thumbnail of Fat and protein content in Turkish hazelnut (Corylus colurna L.) in Kastamonu province

Ana yurdu ve dogal yayilis alani Anadolu olan Turk findigi ( Corylus colurna L.), Turkiye icin on... more Ana yurdu ve dogal yayilis alani Anadolu olan Turk findigi ( Corylus colurna L.), Turkiye icin onemli findik turlerinden biridir. Ustelik, ekstrem iklim ve fakir toprak kosullarina adaptasyon kabiliyeti sayesinde, iklim degisikligi senaryolarina karsi anahtar bir orman agaci turu rolundedir. Turkiye'nin Avrupa-Sibirya flora elementi olan Turk findigi, IUCN Kirmizi Listesine gore " Dusuk Risk " kategorisinde bir taksondur. Turkiye'de daginik-parcali, izole halde kucuk mescere, grup, kume ve yer yer munferit bireyler halinde yayilis gosteren Turk findigi, Anadolu'daki en yogun yayilisini Kuzeybati Anadolu ormanlarinda gerceklestirmektedir. Turk findiginin meyveleri, Dunya'nin farkli bolgelerinde geleneksel tibbi degerli bir ilac olarak kullanilmaktadir. Bu calismada, Kastamonu yoresindeki dort Turk findigi populasyonun (Agli-Tunuslar, Agli-Musellimler, Arac-Guzluk ve Tosya-Kucuksekiler) meyvelerinin yag ve protein icerikleri bakimindan populasyon ici ve arasi...

Research paper thumbnail of Genom Projeleri ve Gen Ailelerinin Biyoinformatik Analizleri

Research paper thumbnail of Orman Gen Kaynakları ve Kastamonu Ormanları

Research paper thumbnail of Growth Stages of Bare Rooted Seedlings of Taurus Cedar and Anatolian Black Pine in Eskisehir Forest Nursery Conditions

Bu calismada, Eskisehir Orman fidanligi kosullarinda yetistirilen farkli orijinlere ait 1+0 ve 2+... more Bu calismada, Eskisehir Orman fidanligi kosullarinda yetistirilen farkli orijinlere ait 1+0 ve 2+0 yasli ciplak koklu fidanlarda rutin yetistirme islemleri sonucu; “Kuru madde degisim” yontemiyle “fidan gelisim donemleri” belirlenmistir. Fidanlar, ozel herhangi bir isleme tabi tutulmadan normal yetisme/yetistirme kosullari altinda; ilk gelisme, hizli gelisme, duraklama, odunlasma ve gercek durgunluk donemlerinin yilin hangi donemiyle ortustugu incelenmistir. Fidanlik kosullarina ve ture ozgu gelisim evrelerinin tespitiyle; uygun gubreleme ve sulama rejimleri, kok kesimi, seyreltme, ot alma zamani ve sokum gibi kulturel islemler icin en uygun zamanin belirlenmesi amaclanmistir. Calisma sonucuna gore; 2+0 yasli Toros Sediri fidanlari icin fidan gelisim donemleri; Mart basi - Nisan ortasi durgimluktan cikis donemi, Mayis ayi basi — Temmuz ayi basi gelisme ve hizli gelisme donemi, Temmuz ayi sonu - Agustos ayi ortasi yavaslama donemi, Eylul ayi ortasindan itibaren odunlasma donemine gir...

Research paper thumbnail of Antioxidant variability of the seeds in core and marginal populations of taurus cedar (Cedrus libani A. Rich.)

Šumarski list, Dec 14, 2018

Genetic diversity is the basis for adaptation and survival of tree species under changing environ... more Genetic diversity is the basis for adaptation and survival of tree species under changing environmental conditions, representing the key issue of stability and productivity of forest ecosystems. In this study, core and marginal populations of Taurus cedar (Cedrus libani A. Rich.) were investigated due to their importance in gene conservation. Assessment of genetic diversity in isolated populations is of great importance for the conservation and improvement programs. Under global climate change conditions, they may possess genotypes of future adaptive potential. The aim of this study is to determine the amount of proline to understand water deficiency stress of the population, total soluble proteins, MDA, H 2 O 2 , α-amylase, the variability of antioxidant as CAT, SOD, APX and GuPX of Taurus Cedar seeds fromfive core populations (Kahramanmaraş-Andırın/Elmadağı (AND), Adana-Pozantı/ Pozantı (POZ), Mersin-Anamur/Abanoz (ANA), Antalya-Finike/Aykırıçay (FIN) and Antalya-Kaş/Karaçay (KAS)) and one marginal provenance (Amasya-Tokat-Niksar/Çatalan (NIK)) in Turkey. According to the results, a significant difference was detected between populations. Significantly higher amounts of proline were detected for ANA (7,46 µmol/g) and POZ (7,22 µmol/g) populations, whereas the lowest amounts of proline were detected in KAS (3,98 µmol/g) population, which represent the optimal distribution of Taurus cedar. This finding indicates that POZ and ANA populations, in the transition zone from Mediterranean region to steppe territory, are more resistant to the frost, than the other populations. The highest α-amilase enzyme amount was detected in POZ population, growing in the optimum range for Taurus cedar. Significantly higher levels of H 2 O 2 were detected in NIK (11,97 µmol/g) and ANA (11,60 µmol/g). This is an indication of higher levels of oxidative stress in the seed samples of these populations. With the present research it's verified that, enzymes such as SOD, CAT, GuPX and APX, controlling reactive oxygen species (ROS) levels in plant cells, are the elements of the antioxidant defence system functioning as protective mechanisms for plants against stress conditions. From the practical point of view, improvement in afforestation performance can be achieved on the steppe of Central Anatolia Region holding the potential afforestation areas of Turkey, through use of forest reproductive materials from POZ and ANA stands with their higher resistance against stress, and NIK as an isolated and marginal population.

Research paper thumbnail of Preliminary results of Turkish hazelnut (Corylus colurna L.) populations for testing the nut characteristics

ABI Genetika, 2018

This paper aims to identify the hazelnut characteristics of four different populations (Ağlı-Tunu... more This paper aims to identify the hazelnut characteristics of four different populations (Ağlı-Tunuslar, Ağlı-Müsellimler, Araç-Güzlük and Tosya-Küçüksekiler) in the North Western Black Sea Region of Turkey, one of the most important areas of economic interest for this species. There, the Turkish hazel (Corylus colurna L.) grows in its optimal conditions and reveals relatively high inter-population and intra-population variation in terms of nut characteristics. With the purpose of assessing variation, measurements were performed in four populations in Kastamonu district on 14 different nut characteristics (number of nuts per cluster, nut length (mm), nut width (mm), nut thickness (mm), shell thickness (mm), nut size (mm), nut shape, compression index, nut weight (g), kernel length (mm), kernel width (mm), kernel thickness (mm), kernel weight (g) and kernel ratio (%) of representative samples of the populations. Significant differences were found out among populations with regard to all of nut characteristics (p<0.05). The four populations have created two groups, population of Ağlı-Tunuslar and the others, according to cluster analysis. The closest populations have been Tosya-Küçüksekiler and Araç-Güzlük in terms of nut characteristics. According to the results 670

Research paper thumbnail of Age-Related Changes of Some Chemical Components in the Leaves of Oriental Beech (Fagus orientalis Lipsky.)

South-east European forestry

Background and Purpose: This study presents the analysis of photosynthetic pigments, proline, tot... more Background and Purpose: This study presents the analysis of photosynthetic pigments, proline, total soluble protein, total amino acids, glucose, sucrose, total soluble sugars, total amount of phenolic compounds and flavonoids, malondialdehyde (MDA) and hydrogen peroxide (H2O2) concentration in the leaf samples collected from oriental beech trees, which are naturally spread in Kastamonu Province, Turkey, with differing ages, enzyme activities of ascorbate peroxidase (APX), catalase (CAT) and superoxide dismutase (SOD). Material and Methods: The research was carried out on oriental beech trees (Fagus orientalis L.) of different ages located at 1300 m high elevation in Ahlat Village of Kastamonu Province, Turkey. Oriental beech trees of different ages (≥25, ≥50, ≥100, ≥200 and ≥600 years-old) constituted the material of this study. In leaf samples taken from trees of different ages, photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll and carotenoid), proline, total...

Research paper thumbnail of Identification and expression profiling of all Hsp family member genes under salinity stress in different poplar clones

Gene

Heat shock proteins (Hsps) play a key role for regulation of the changes during different stress ... more Heat shock proteins (Hsps) play a key role for regulation of the changes during different stress conditions including salinity, drought, heavy metal and extreme temperature. Molecular based studies on the response mechanisms of forest trees to abiotic stresses started in 2006 when Populus trichocarpa genome sequence was completed as a model tree species. In recent years, bioinformatic analyzes have been carried out to determine functional gene regions of tree species. In this study, sHsp, Hsp40, Hsp60, Hsp90 and Hsp100 gene family members were identified in poplar genome. Some bioinformatics analyses were conducted, such as: identification of DNA/protein sequences, chromosomal localization, gene structure, calculation of genomic duplications, determination of phylogenetic groups, examination of protected motif regions, identification of gene ontology categories, modeling of protein 3D structure, determination of miRNA targeting genes, examination of sHsp, Hsp40, Hsp60, Hsp90 and Hsp100 gene family members in transcriptome data during salinity stress. As a result of bioinformatic analyzes made on P. trichocarpa genome; 60, 145, 49, 34, 12 and 90 genes belonging to members of sHsp, Hsp40, Hsp60, Hsp70, Hsp90 and Hsp100 protein families were firstly defined within the scope of this study. A total of 390 genes belonging to all Hsps gene families were characterized using different bioinformatics tools. In addition, salinity stress was applied to Populus tremula L. (Samsun) naturally grown in Turkey, Hybrid poplar species I-214 (Populus euramericana Dode. Guinier) and Black Poplar species (Populus nigra L.), Geyve and N.03.368.A clones. The expression levels of the selected Hsps genes were determined by the qRT-PCR method. After salt stress application in various poplar clones, expression levels of genes including PtsHsp-11, PtsHsp-21, PtsHsp-36, PtHsp40-113, PtHsp40-117, PtHsp60-31, PtHsp60-33, PtHsp60-38, PtHsp60-49, PtHsp70-09, PtHsp70-12, 33, PtHsp90-09, PtHsp90-12, PtHsp100-21, and PtHsp100-75 were increased. The role of the Hsps genes during salt stress has been revealed. Together with detailed bioinformatics analyses, gene expression analysis greatly contributes to understand functions of these gene family members. This research serves as a blueprint for future studies and offers a significant clue for the further study of the functions of this important gene family. Moreover, determined genes in this study can also be used for cloning studies in agricultural practices.

Research paper thumbnail of Türkiye’deki Toros sediri (Cedrus libani A. Rich.) ağaçlandırma sahalarının iklim tipi açısından değerlendirilmesi

Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi

Türkiye'deki Toros sediri (Cedrus libani A. Rich.) ağaçlandırma sahalarının iklim tipi açısından ... more Türkiye'deki Toros sediri (Cedrus libani A. Rich.) ağaçlandırma sahalarının iklim tipi açısından değerlendirilmesi Evaluation of Taurus cedar (Cedrus libani A. Rich.

Research paper thumbnail of Utilization of Biotechnology on Some Forest Trees in Turkey

South-east European forestry, 2014

SEEFOR 5 (2): e1-e10 e1

Research paper thumbnail of Eskişehir Orman Fidanlığı Koşullarında Yetiştirilen Çıplak Köklü Toros Sediri ve Anadolu Karaçamı Fidanlarının Gelişim Dönemleri Growth Stages of Bare Rooted Seedlings of Taurus Cedar and Anatolian Black Pine in Eskişehir Forest Nursery Conditions

zet Bu çalışmada, Eskişehir Orman fidanlığı koşullarında yetiştirilen farklı orijinlere ait 1+0 v... more zet Bu çalışmada, Eskişehir Orman fidanlığı koşullarında yetiştirilen farklı orijinlere ait 1+0 ve 2+0 yaşlı çıplak köklü fidanlarda rutin yetiştirme işlemleri sonucu; "Kuru madde değişim" yöntemiyle "fidan gelişim dönemleri" belirlenmiştir. Fidanlar, özel herhangi bir işleme tabi tutulmadan normal yetişme/yetiştirme koşulları altında; ilk gelişme, hızlı gelişme, duraklama, odunlaşma ve gerçek durgunluk dönemlerinin yılın hangi dönemiyle örtüştüğü incelenmiştir. Fidanlık koşullarına ve türe özgü gelişim evrelerinin tespitiyle; uygun gübreleme ve sulama rejimleri, kök kesimi, seyreltme, ot alma zamanı ve söküm gibi kültürel işlemler için en uygun zamanın belirlenmesi amaçlanmıştır. Çalışma sonucuna göre; 2+0 yaşlı Toros Sediri fidanları için fidan gelişim dönemleri; Mart başı -Nisan ortası durgunluktan çıkış dönemi, Mayıs ayı başı – Temmuz ayı başı gelişme ve hızlı gelişme dönemi, Temmuz ayı sonu -Ağustos ayı ortası yavaşlama dönemi, Eylül ayı ortasından itibaren ...

Research paper thumbnail of Türkiye Orman Fidanlıklarında Yetiştirilen Süs Bitkilerinin Üretim Teknikleri Esra Nurten Yer*, Sezgin Ayan Kastamonu Üniversitesi, Orman Fakültesi Orman Mühendisliği Bölümü, 37200 Kastamonu * esranurtenyer@gmail.com

Özet Son yıllarda yeni bir yapılanma gerçekleĢtiren Orman ve Su ĠĢleri Bakanlığı, faaliyetlerini ... more Özet Son yıllarda yeni bir yapılanma gerçekleĢtiren Orman ve Su ĠĢleri Bakanlığı, faaliyetlerini 27 Orman Bölge Müdürlüğü marifetiyle gerçekleĢtirmektedir. Bölge müdürlükleri bünyesinde ise Fidanlık Müdürlükleri tohum ve fidan üretim çalıĢmalarına yoğunlaĢmıĢ teknik ve idari birimleridir. Ülkemizde ilk kurulan orman fidanlıkları sırasıyla; Ankara, Ġzmir–KarĢıyaka ve Ġstanbul–Bahçeköy orman fidanlıklarıdır. Mülga Ağaçlandırma ve Erozyon Kontrolü Genel Müdürlüğünün kayıtları yıllar itibariyle incelendiğinde; fidan yetiĢtiriciliğinde önemli atılımların gerçekleĢtirilmiĢ olduğu, hatta artan fidan ihtiyacının karĢılanabilmesi için kapalı ve açık alan fidanlık iĢletmeleri kurulmuĢtur. Günümüzde ormancılık sektörü içerisinde fidancılık önemli bir iĢ kolu durumundadır. Orman Genel Müdürlüğüne bağlı olarak faaliyet gösteren orman fidanlıklarında son beĢ yılda üretilen süs bitkilerinin; 55 familya ve 115 cinse ait toplam 210 takson olduğu saptanmıĢtır. Ġzmir, Balıkesir ve Adapazarı Orman Fida...

Research paper thumbnail of Drought-Responsive Hsp70 Gene Analysis in Populus at Genome-Wide Level

The heat shock protein 70 (Hsp70) family members are known as molecular chaperones. They play a c... more The heat shock protein 70 (Hsp70) family members are known as molecular chaperones. They play a crucial role in protecting plant cells and tissues from thermal or abiotic stress through protein folding and in assembly, stabilization, activation, and degradation processes. Although many studies have been performed to identify molecular functions of individual family members, there is a limited study on genome-wide identification and characterizations of Hsps in the Populus model tree genus. We have identified 34 poplar Hsp70 genes, which were phylogenetically clustered into three major groups. Gene structure and motif composition are relatively conserved in each group. Mainly tandem and infrequently segmental duplications have a significant role in poplar Hsp70 gene expansion. The in silico microRNA (miRNA) and target transcript analyses identified that a total of 19 PtHsp70 genes were targeted by 27 plant miRNAs. PtHSP70-14 and PtHSP70-33 are the most targeted by miR390 and miR414 family members, respectively. For determination of drought response to Hsp70 genes, publicly available RNA-seq data were analyzed. Poplar Hsp70s are differentially expressed upon exposure to different drought stress conditions. Expression analysis of PtHsp70 genes was also examined under drought stress in drought-sensitive and drought-resistant Populus clones with quantitative real-time PCR (qRT-PCR). PtHsp70-16 and PtHsp70-26 genes might provide adaptation to drought stress for both clones. Because of high expression responses to drought in only resistant Populus clone, PtHsp70-25 and PtHsp70-33 genes might be used for determination of drought-tolerant clones for molecular breeding studies. This research provides a fundamental clue for contribution of PtHsp70s to drought tolerance in poplar.

Research paper thumbnail of Genome-wide identification and comparative expression analysis of LEA genes in watermelon and melon genomes

Physiology and Molecular Biology of Plants, 2017

Research paper thumbnail of Türkiye Orman Fidanlıklarında Yetiştirilen Süs Bitkilerinin Üretim Teknikleri Esra Nurten Yer*, Sezgin Ayan Kastamonu Üniversitesi, Orman Fakültesi Orman Mühendisliği Bölümü, 37200 Kastamonu * esranurtenyer@gmail.com

Süs bitkileri sektörü ekonomik olarak son yıllarda büyük ilerleme göstermiĢtir. Bu ilerlemede kon... more Süs bitkileri sektörü ekonomik olarak son yıllarda büyük ilerleme göstermiĢtir. Bu ilerlemede konu ile ilgili yapılan bilimsel toplantıların, sempozyum ve kongrelerin katkısı önemlidir. Bu tip organizasyonlarda; araĢtırmacılar, öğretim üyeleri, özel sektör ve sivil toplum kuruluĢları süs bitkileri sektöründeki sorunları ve çözüm önerilerini, bilimsel ve teknolojik geliĢmeleri birlikte tartıĢıp değerlendirme imkânı bulmaktadır. Bu değerlendirmelerden elde edilen sonuçlar hem Ar-Ge faaliyetlerinin etkinliğinin artması hem de sektörün geliĢmesi açısından büyük önem arz etmektedir.

Research paper thumbnail of Identification and expression analysis of LEA gene family members in cucumber genome

Plant Growth Regulation, 2016

LEA (late embryogenesis abundant) proteins are firstly discovered in seeds and then identified in... more LEA (late embryogenesis abundant) proteins are firstly discovered in seeds and then identified in vegetative tissues of different plant species. They are mainly regulated under abiotic stress conditions. Although genome wide studies of different gene family members have been performed in cucumber, there is no such a study for LEA genes. We have identified 79 LEA genes in the cucumber genome. Based on phylogenetic analysis, CsLEA genes could be classified into seven groups in which structural motifs are relatively conserved. Tandem duplications play an important role in cucumber genome for LEA gene expansion. Orthologous and chromosomal relationships of CsLEA genes were observed based on comparative mapping analysis with other species. The in silico micro-RNA (miRNA) target analyses indicated that 37 CsLEA genes were targeted by different miRNAs, especially mir854 and mir414 are the most abundant identified ones. Public available RNA-seq data were analyzed for expression analysis of CsLEA genes in different tissues of cucumber. According to genome-wide expression analysis, nine CsLEA genes showed higher expression profiles in all tissues. The expression profiles of ten CsLEA genes in the root and leaf tissues of drought-stressed cucumber were examined using qRT-PCR. Among them, CsLEA-54 induced after stress application in leaf and root tissues and might provide adaptation to drought stress for cucumber. CsLEA-09, CsLEA-32 and CsLEA-57 genes responded to drought after 3 h later and might be considered as early response genes to water limitation. This research could help us to improve understanding of contribution of CsLEAs to drought tolerance in cucumber.

Research paper thumbnail of Drought-Responsive Hsp70 Gene Analysis in Populus at Genome-Wide Level

Plant Molecular Biology Reporter, 2015

The heat shock protein 70 (Hsp70) family members are known as molecular chaperones. They play a c... more The heat shock protein 70 (Hsp70) family members are known as molecular chaperones. They play a crucial role in protecting plant cells and tissues from thermal or abiotic stress through protein folding and in assembly, stabilization, activation, and degradation processes. Although many studies have been performed to identify molecular functions of individual family members, there is a limited study on genome-wide identification and characterizations of Hsps in the Populus model tree genus. We have identified 34 poplar Hsp70 genes, which were phylogenetically clustered into three major groups. Gene structure and motif composition are relatively conserved in each group. Mainly tandem and infrequently segmental duplications have a significant role in poplar Hsp70 gene expansion. The in silico microRNA (miRNA) and target transcript analyses identified that a total of 19 PtHsp70 genes were targeted by 27 plant miRNAs. PtHSP70-14 and PtHSP70-33 are the most targeted by miR390 and miR414 family members, respectively. For determination of drought response to Hsp70 genes, publicly available RNA-seq data were analyzed. Poplar Hsp70s are differentially expressed upon exposure to different drought stress conditions. Expression analysis of PtHsp70 genes was also examined under drought stress in drought-sensitive and drought-resistant Populus clones with quantitative real-time PCR (qRT-PCR). PtHsp70-16 and PtHsp70-26 genes might provide adaptation to drought stress for both clones. Because of high expression responses to drought in only resistant Populus clone, PtHsp70-25 and PtHsp70-33 genes might be used for determination of drought-tolerant clones for molecular breeding studies. This research provides a fundamental clue for contribution of PtHsp70s to drought tolerance in poplar.

Research paper thumbnail of The occurrence of heart rot on Abies nordmanniana subsp. bornmülleriana Mattf. trees in relation to altitude

Kastamonu University Journal of Forestry Faculty, 2012

Fir is the taxon subjected to the selection systems in Turkish forestry. In selection forests, ta... more Fir is the taxon subjected to the selection systems in Turkish forestry. In selection forests, target diameter is the main criterion for planning and management. In this study the aim is to determine the formation and level of the heart rot and target diameter depending on aspect and altitude for Abies nordmanniana subsp. bornmülleriana Mattf. For this purpose, the fir trees with maximum diameters were cut and the situation of heart rot at various altitude levels (900-1,200 m, 1,200-1,500 m, and 1,500-1,800 m) and aspects was determined. The formation of heart rot starts at low altitudes and this defect was found nearly on all fir trees of target diameter of 60 cm which is commonly used in practical Turkish forestry. Target diameter of 60 cm was considered as usable at the altitude of 1,200 m and even higher, at 1,500 m and even more, the usable target diameter is more than 60 cm, as heart rot was not found in the sample trees with target diameters about 70-80 cm. In addition to thi...

Research paper thumbnail of Fat and protein content in Turkish hazelnut (Corylus colurna L.) in Kastamonu province

Ana yurdu ve dogal yayilis alani Anadolu olan Turk findigi ( Corylus colurna L.), Turkiye icin on... more Ana yurdu ve dogal yayilis alani Anadolu olan Turk findigi ( Corylus colurna L.), Turkiye icin onemli findik turlerinden biridir. Ustelik, ekstrem iklim ve fakir toprak kosullarina adaptasyon kabiliyeti sayesinde, iklim degisikligi senaryolarina karsi anahtar bir orman agaci turu rolundedir. Turkiye'nin Avrupa-Sibirya flora elementi olan Turk findigi, IUCN Kirmizi Listesine gore " Dusuk Risk " kategorisinde bir taksondur. Turkiye'de daginik-parcali, izole halde kucuk mescere, grup, kume ve yer yer munferit bireyler halinde yayilis gosteren Turk findigi, Anadolu'daki en yogun yayilisini Kuzeybati Anadolu ormanlarinda gerceklestirmektedir. Turk findiginin meyveleri, Dunya'nin farkli bolgelerinde geleneksel tibbi degerli bir ilac olarak kullanilmaktadir. Bu calismada, Kastamonu yoresindeki dort Turk findigi populasyonun (Agli-Tunuslar, Agli-Musellimler, Arac-Guzluk ve Tosya-Kucuksekiler) meyvelerinin yag ve protein icerikleri bakimindan populasyon ici ve arasi...

Research paper thumbnail of Genom Projeleri ve Gen Ailelerinin Biyoinformatik Analizleri

Research paper thumbnail of Orman Gen Kaynakları ve Kastamonu Ormanları

Research paper thumbnail of Growth Stages of Bare Rooted Seedlings of Taurus Cedar and Anatolian Black Pine in Eskisehir Forest Nursery Conditions

Bu calismada, Eskisehir Orman fidanligi kosullarinda yetistirilen farkli orijinlere ait 1+0 ve 2+... more Bu calismada, Eskisehir Orman fidanligi kosullarinda yetistirilen farkli orijinlere ait 1+0 ve 2+0 yasli ciplak koklu fidanlarda rutin yetistirme islemleri sonucu; “Kuru madde degisim” yontemiyle “fidan gelisim donemleri” belirlenmistir. Fidanlar, ozel herhangi bir isleme tabi tutulmadan normal yetisme/yetistirme kosullari altinda; ilk gelisme, hizli gelisme, duraklama, odunlasma ve gercek durgunluk donemlerinin yilin hangi donemiyle ortustugu incelenmistir. Fidanlik kosullarina ve ture ozgu gelisim evrelerinin tespitiyle; uygun gubreleme ve sulama rejimleri, kok kesimi, seyreltme, ot alma zamani ve sokum gibi kulturel islemler icin en uygun zamanin belirlenmesi amaclanmistir. Calisma sonucuna gore; 2+0 yasli Toros Sediri fidanlari icin fidan gelisim donemleri; Mart basi - Nisan ortasi durgimluktan cikis donemi, Mayis ayi basi — Temmuz ayi basi gelisme ve hizli gelisme donemi, Temmuz ayi sonu - Agustos ayi ortasi yavaslama donemi, Eylul ayi ortasindan itibaren odunlasma donemine gir...

Research paper thumbnail of Antioxidant variability of the seeds in core and marginal populations of taurus cedar (Cedrus libani A. Rich.)

Šumarski list, Dec 14, 2018

Genetic diversity is the basis for adaptation and survival of tree species under changing environ... more Genetic diversity is the basis for adaptation and survival of tree species under changing environmental conditions, representing the key issue of stability and productivity of forest ecosystems. In this study, core and marginal populations of Taurus cedar (Cedrus libani A. Rich.) were investigated due to their importance in gene conservation. Assessment of genetic diversity in isolated populations is of great importance for the conservation and improvement programs. Under global climate change conditions, they may possess genotypes of future adaptive potential. The aim of this study is to determine the amount of proline to understand water deficiency stress of the population, total soluble proteins, MDA, H 2 O 2 , α-amylase, the variability of antioxidant as CAT, SOD, APX and GuPX of Taurus Cedar seeds fromfive core populations (Kahramanmaraş-Andırın/Elmadağı (AND), Adana-Pozantı/ Pozantı (POZ), Mersin-Anamur/Abanoz (ANA), Antalya-Finike/Aykırıçay (FIN) and Antalya-Kaş/Karaçay (KAS)) and one marginal provenance (Amasya-Tokat-Niksar/Çatalan (NIK)) in Turkey. According to the results, a significant difference was detected between populations. Significantly higher amounts of proline were detected for ANA (7,46 µmol/g) and POZ (7,22 µmol/g) populations, whereas the lowest amounts of proline were detected in KAS (3,98 µmol/g) population, which represent the optimal distribution of Taurus cedar. This finding indicates that POZ and ANA populations, in the transition zone from Mediterranean region to steppe territory, are more resistant to the frost, than the other populations. The highest α-amilase enzyme amount was detected in POZ population, growing in the optimum range for Taurus cedar. Significantly higher levels of H 2 O 2 were detected in NIK (11,97 µmol/g) and ANA (11,60 µmol/g). This is an indication of higher levels of oxidative stress in the seed samples of these populations. With the present research it's verified that, enzymes such as SOD, CAT, GuPX and APX, controlling reactive oxygen species (ROS) levels in plant cells, are the elements of the antioxidant defence system functioning as protective mechanisms for plants against stress conditions. From the practical point of view, improvement in afforestation performance can be achieved on the steppe of Central Anatolia Region holding the potential afforestation areas of Turkey, through use of forest reproductive materials from POZ and ANA stands with their higher resistance against stress, and NIK as an isolated and marginal population.

Research paper thumbnail of Preliminary results of Turkish hazelnut (Corylus colurna L.) populations for testing the nut characteristics

ABI Genetika, 2018

This paper aims to identify the hazelnut characteristics of four different populations (Ağlı-Tunu... more This paper aims to identify the hazelnut characteristics of four different populations (Ağlı-Tunuslar, Ağlı-Müsellimler, Araç-Güzlük and Tosya-Küçüksekiler) in the North Western Black Sea Region of Turkey, one of the most important areas of economic interest for this species. There, the Turkish hazel (Corylus colurna L.) grows in its optimal conditions and reveals relatively high inter-population and intra-population variation in terms of nut characteristics. With the purpose of assessing variation, measurements were performed in four populations in Kastamonu district on 14 different nut characteristics (number of nuts per cluster, nut length (mm), nut width (mm), nut thickness (mm), shell thickness (mm), nut size (mm), nut shape, compression index, nut weight (g), kernel length (mm), kernel width (mm), kernel thickness (mm), kernel weight (g) and kernel ratio (%) of representative samples of the populations. Significant differences were found out among populations with regard to all of nut characteristics (p<0.05). The four populations have created two groups, population of Ağlı-Tunuslar and the others, according to cluster analysis. The closest populations have been Tosya-Küçüksekiler and Araç-Güzlük in terms of nut characteristics. According to the results 670

Research paper thumbnail of Age-Related Changes of Some Chemical Components in the Leaves of Oriental Beech (Fagus orientalis Lipsky.)

South-east European forestry

Background and Purpose: This study presents the analysis of photosynthetic pigments, proline, tot... more Background and Purpose: This study presents the analysis of photosynthetic pigments, proline, total soluble protein, total amino acids, glucose, sucrose, total soluble sugars, total amount of phenolic compounds and flavonoids, malondialdehyde (MDA) and hydrogen peroxide (H2O2) concentration in the leaf samples collected from oriental beech trees, which are naturally spread in Kastamonu Province, Turkey, with differing ages, enzyme activities of ascorbate peroxidase (APX), catalase (CAT) and superoxide dismutase (SOD). Material and Methods: The research was carried out on oriental beech trees (Fagus orientalis L.) of different ages located at 1300 m high elevation in Ahlat Village of Kastamonu Province, Turkey. Oriental beech trees of different ages (≥25, ≥50, ≥100, ≥200 and ≥600 years-old) constituted the material of this study. In leaf samples taken from trees of different ages, photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll and carotenoid), proline, total...

Research paper thumbnail of Identification and expression profiling of all Hsp family member genes under salinity stress in different poplar clones

Gene

Heat shock proteins (Hsps) play a key role for regulation of the changes during different stress ... more Heat shock proteins (Hsps) play a key role for regulation of the changes during different stress conditions including salinity, drought, heavy metal and extreme temperature. Molecular based studies on the response mechanisms of forest trees to abiotic stresses started in 2006 when Populus trichocarpa genome sequence was completed as a model tree species. In recent years, bioinformatic analyzes have been carried out to determine functional gene regions of tree species. In this study, sHsp, Hsp40, Hsp60, Hsp90 and Hsp100 gene family members were identified in poplar genome. Some bioinformatics analyses were conducted, such as: identification of DNA/protein sequences, chromosomal localization, gene structure, calculation of genomic duplications, determination of phylogenetic groups, examination of protected motif regions, identification of gene ontology categories, modeling of protein 3D structure, determination of miRNA targeting genes, examination of sHsp, Hsp40, Hsp60, Hsp90 and Hsp100 gene family members in transcriptome data during salinity stress. As a result of bioinformatic analyzes made on P. trichocarpa genome; 60, 145, 49, 34, 12 and 90 genes belonging to members of sHsp, Hsp40, Hsp60, Hsp70, Hsp90 and Hsp100 protein families were firstly defined within the scope of this study. A total of 390 genes belonging to all Hsps gene families were characterized using different bioinformatics tools. In addition, salinity stress was applied to Populus tremula L. (Samsun) naturally grown in Turkey, Hybrid poplar species I-214 (Populus euramericana Dode. Guinier) and Black Poplar species (Populus nigra L.), Geyve and N.03.368.A clones. The expression levels of the selected Hsps genes were determined by the qRT-PCR method. After salt stress application in various poplar clones, expression levels of genes including PtsHsp-11, PtsHsp-21, PtsHsp-36, PtHsp40-113, PtHsp40-117, PtHsp60-31, PtHsp60-33, PtHsp60-38, PtHsp60-49, PtHsp70-09, PtHsp70-12, 33, PtHsp90-09, PtHsp90-12, PtHsp100-21, and PtHsp100-75 were increased. The role of the Hsps genes during salt stress has been revealed. Together with detailed bioinformatics analyses, gene expression analysis greatly contributes to understand functions of these gene family members. This research serves as a blueprint for future studies and offers a significant clue for the further study of the functions of this important gene family. Moreover, determined genes in this study can also be used for cloning studies in agricultural practices.

Research paper thumbnail of Türkiye’deki Toros sediri (Cedrus libani A. Rich.) ağaçlandırma sahalarının iklim tipi açısından değerlendirilmesi

Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi

Türkiye'deki Toros sediri (Cedrus libani A. Rich.) ağaçlandırma sahalarının iklim tipi açısından ... more Türkiye'deki Toros sediri (Cedrus libani A. Rich.) ağaçlandırma sahalarının iklim tipi açısından değerlendirilmesi Evaluation of Taurus cedar (Cedrus libani A. Rich.

Research paper thumbnail of Utilization of Biotechnology on Some Forest Trees in Turkey

South-east European forestry, 2014

SEEFOR 5 (2): e1-e10 e1

Research paper thumbnail of Eskişehir Orman Fidanlığı Koşullarında Yetiştirilen Çıplak Köklü Toros Sediri ve Anadolu Karaçamı Fidanlarının Gelişim Dönemleri Growth Stages of Bare Rooted Seedlings of Taurus Cedar and Anatolian Black Pine in Eskişehir Forest Nursery Conditions

zet Bu çalışmada, Eskişehir Orman fidanlığı koşullarında yetiştirilen farklı orijinlere ait 1+0 v... more zet Bu çalışmada, Eskişehir Orman fidanlığı koşullarında yetiştirilen farklı orijinlere ait 1+0 ve 2+0 yaşlı çıplak köklü fidanlarda rutin yetiştirme işlemleri sonucu; "Kuru madde değişim" yöntemiyle "fidan gelişim dönemleri" belirlenmiştir. Fidanlar, özel herhangi bir işleme tabi tutulmadan normal yetişme/yetiştirme koşulları altında; ilk gelişme, hızlı gelişme, duraklama, odunlaşma ve gerçek durgunluk dönemlerinin yılın hangi dönemiyle örtüştüğü incelenmiştir. Fidanlık koşullarına ve türe özgü gelişim evrelerinin tespitiyle; uygun gübreleme ve sulama rejimleri, kök kesimi, seyreltme, ot alma zamanı ve söküm gibi kültürel işlemler için en uygun zamanın belirlenmesi amaçlanmıştır. Çalışma sonucuna göre; 2+0 yaşlı Toros Sediri fidanları için fidan gelişim dönemleri; Mart başı -Nisan ortası durgunluktan çıkış dönemi, Mayıs ayı başı – Temmuz ayı başı gelişme ve hızlı gelişme dönemi, Temmuz ayı sonu -Ağustos ayı ortası yavaşlama dönemi, Eylül ayı ortasından itibaren ...

Research paper thumbnail of Türkiye Orman Fidanlıklarında Yetiştirilen Süs Bitkilerinin Üretim Teknikleri Esra Nurten Yer*, Sezgin Ayan Kastamonu Üniversitesi, Orman Fakültesi Orman Mühendisliği Bölümü, 37200 Kastamonu * esranurtenyer@gmail.com

Özet Son yıllarda yeni bir yapılanma gerçekleĢtiren Orman ve Su ĠĢleri Bakanlığı, faaliyetlerini ... more Özet Son yıllarda yeni bir yapılanma gerçekleĢtiren Orman ve Su ĠĢleri Bakanlığı, faaliyetlerini 27 Orman Bölge Müdürlüğü marifetiyle gerçekleĢtirmektedir. Bölge müdürlükleri bünyesinde ise Fidanlık Müdürlükleri tohum ve fidan üretim çalıĢmalarına yoğunlaĢmıĢ teknik ve idari birimleridir. Ülkemizde ilk kurulan orman fidanlıkları sırasıyla; Ankara, Ġzmir–KarĢıyaka ve Ġstanbul–Bahçeköy orman fidanlıklarıdır. Mülga Ağaçlandırma ve Erozyon Kontrolü Genel Müdürlüğünün kayıtları yıllar itibariyle incelendiğinde; fidan yetiĢtiriciliğinde önemli atılımların gerçekleĢtirilmiĢ olduğu, hatta artan fidan ihtiyacının karĢılanabilmesi için kapalı ve açık alan fidanlık iĢletmeleri kurulmuĢtur. Günümüzde ormancılık sektörü içerisinde fidancılık önemli bir iĢ kolu durumundadır. Orman Genel Müdürlüğüne bağlı olarak faaliyet gösteren orman fidanlıklarında son beĢ yılda üretilen süs bitkilerinin; 55 familya ve 115 cinse ait toplam 210 takson olduğu saptanmıĢtır. Ġzmir, Balıkesir ve Adapazarı Orman Fida...

Research paper thumbnail of Drought-Responsive Hsp70 Gene Analysis in Populus at Genome-Wide Level

The heat shock protein 70 (Hsp70) family members are known as molecular chaperones. They play a c... more The heat shock protein 70 (Hsp70) family members are known as molecular chaperones. They play a crucial role in protecting plant cells and tissues from thermal or abiotic stress through protein folding and in assembly, stabilization, activation, and degradation processes. Although many studies have been performed to identify molecular functions of individual family members, there is a limited study on genome-wide identification and characterizations of Hsps in the Populus model tree genus. We have identified 34 poplar Hsp70 genes, which were phylogenetically clustered into three major groups. Gene structure and motif composition are relatively conserved in each group. Mainly tandem and infrequently segmental duplications have a significant role in poplar Hsp70 gene expansion. The in silico microRNA (miRNA) and target transcript analyses identified that a total of 19 PtHsp70 genes were targeted by 27 plant miRNAs. PtHSP70-14 and PtHSP70-33 are the most targeted by miR390 and miR414 family members, respectively. For determination of drought response to Hsp70 genes, publicly available RNA-seq data were analyzed. Poplar Hsp70s are differentially expressed upon exposure to different drought stress conditions. Expression analysis of PtHsp70 genes was also examined under drought stress in drought-sensitive and drought-resistant Populus clones with quantitative real-time PCR (qRT-PCR). PtHsp70-16 and PtHsp70-26 genes might provide adaptation to drought stress for both clones. Because of high expression responses to drought in only resistant Populus clone, PtHsp70-25 and PtHsp70-33 genes might be used for determination of drought-tolerant clones for molecular breeding studies. This research provides a fundamental clue for contribution of PtHsp70s to drought tolerance in poplar.