Magdy Mousa | King AbdulAziz University (KAU) Jeddah, Saudi Arabia (original) (raw)
Papers by Magdy Mousa
Tomato is an important vegetable crop and its production is adversely affected by leaf curl disea... more Tomato is an important vegetable crop and its
production is adversely affected by leaf curl disease caused
by begomovirus. Leaf curl disease is a serious concern for
tomato crops caused by begomovirus in Jeddah, Kingdom
of Saudi Arabia. Tomato leaf curl disease has been shown
to be mainly caused either by tomato leaf curl Sudan virus
or tomato yellow leaf curl virus as well as tomato leaf curl
Oman virus. Many tomato plants infected with monopartite
begomoviruses were also found to harbor a symptom
enhancing betasatellites. Here we report the association of
tomato leaf curl Sudan virus causing leaf curl disease of
tomato in Jeddah, Kingdom of Saudi Arabia. The complete
genome sequence analysis showed highest (99.9 %) identity
with tomato leaf curl Sudan virus causing leaf curl
disease in Arabian Peninsula. In phylogenetic relationships
analysis, the identified virus formed closest cluster with
tomato leaf curl Sudan virus. In recombination analysis
study, the major parent was identified as tomato leaf curl
Sudan virus. Findings of this study strongly supports the
associated virus is a variant of tomato leaf curl Sudan virus
causing disease in Sudan, Yemen and Arabian Peninsula.
The betasatellites sequence analysis showed highest identity
(99.8 %) with tomato leaf curl betasatellites-Amaranthus-Jeddah.
The phylogenetic analysis result based on
betasatellites formed closed cluster with tomato yellow leaf curl Oman betasatellites. The importance of these findings
and occurrence of begomovirus in new geographic regions
causing leaf curl disease of tomato in Jeddah, Kingdom of
Saudi Arabia are discussed.
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Acta Diabetologica, 1999
Type 2 (non-insulin-dependent) diabetes is emerging as a leading chronic non-communicable disease... more Type 2 (non-insulin-dependent) diabetes is emerging as a leading chronic non-communicable disease among the adult Kuwaiti population. Based on the World Health Organization and similar reports the projected estimates for subjects suffering from type 2 diabetes by the years 2000 and 2010 show a striking tendency to high rates of the disease in our area. We report the prevalence rates of glucose intolerance among a relatively young adult Kuwaiti population below 50 years of age and the effect of implementing the recent 1997 American Diabetes Association diagnostic criteria on the frequency of type 2 diabetes, impaired glucose tolerance and impaired fasting glucose among this group. The overall prevalence rate for the three categories of glucose intolerance reached as high as 15.8% (95% CI, 14.2 to 17.4). Age, though all subjects were below 50 years, parental history of type 2 diabetes, diastolic blood pressure and serum triglycerides were found to be significant associated risk factors for the development of type 2 diabetes. Obesity was an apparent significant factor associated with the three forms of glucose intolerance (p < 0.001). Obesity and physical inactivity were documented in both non-diabetic and more so in diabetic Kuwaiti adults, which should form the basis of any immediate intervention programme. An integrated approach to the prevention of the described critical risk factors associated with type 2 diabetes is highly recommended in Kuwait. Research, focused on genetics of type 2 diabetes in the highly susceptible Kuwaiti population, should be planned.
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Tomato (Solanum lycopersicum L.) production is severely affected by many diseases caused by many ... more Tomato (Solanum lycopersicum L.) production is severely affected by many diseases caused by many pathogens and among them viruses are the most serious pathogen. Begomoviruses causes yellow mosaic and leaf curl disease of tomato in the tropical, subtropical, temperate, and even semiarid regions. Yellow leaf curl disease is caused by Tomato yellow leaf curl virus belonging to the genus Begomovirus of the family Geminiviridae. In this study, naturally infected tomato leaf samples were collected during field survey and causal virus was identified by PCR using tomato yellow leaf curl virus-specific primers and transmitted by whiteflies to healthy tomato seedlings. The full-length viral genome was amplified by rolling circle amplification technology while betasatellites from viral genome were amplified by PCR using universal betasatellites primers. The full-length viral genome (~2.7kb) and betasatellites (~1.4kb) were cloned and sequenced bi-directionally. The generated sequences were assembled and analyzed to find out the genetic variability by using bioinformatics tools and the genetic variability and phylogenetic relationships with selected begomoviruses were analyzed. The complete viral genome sequences showed highest (99.5%) similarity with an isolate of Tomato Yellow leaf curl virus-Jizan 103 isolate103 and 92.8% similarity with Tomato Yellow leaf curl virus-Al-Qasim isolate while the lowest (71.6%) were found with Tomato Yellow leaf curl virus-Egypt isolate. The newly identified virus formed the closest cluster with Tomato yellow leaf curl virus isolates from Jizan and Al-Qasim, Saudi Arabia. On the basis of sequence similarity and phylogenetic relationship and recombination analysis, it is concluded that the virus causing tomato yellow leaf curl disease could be a variant of tomato yellow leaf curl virus either from Jizan or Al-Qasim isolate circulating in the Kingdom of Saudi Arabia.
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Abstract: A field experiment was conducted during two cropping seasons 2012 and 2013 at the Agric... more Abstract: A field experiment was conducted during two cropping seasons 2012 and 2013 at the Agriculture
Research Station belonging to King Abdulaziz University to study the effects of set sizes (SS) and irrigation system
(IS) on growth, yield, doubling and early bolting of three onion cultivars. Three sizes of sets, small (0.5-1.5cm),
medium (1.5-2.5cm) and large (2.5-3.5cm) of onion cultivars ‘Red Amposta’, ‘Giza 6’ and ‘Texas
502’werecultivated using surface and subsurface drip irrigation systems. All experiments were laid out in
Randomized Complete Block Design (RCBD) using 3 replicates. The results showed that growth, yield and bulbs
quality of the tested onions cultivars were significantly affected by the irrigation systems and sizes of sets and their
interactions. The surface drip irrigation (IS1) produced the highest plant height and no. of leaves/plant, highest no.
of doubling, split and early bolting bulbs/plot. The subsurface drip irrigation (IS2) produced the highest average
weight and diameter of single marketable bulbs, no. of marketable bulbs/plot, total yield of marketable, doubling,
split and early bolting bulbs (ton/ha). The sizes of sets 2.5-3.5cm (SS3) significantly increased plant height (cm), no.
of leaves/plant, days to maturity, number (per plot) and total yield (ton/ha) of doubling, split and early bolting bulbs.
Using the medium sizes of sets(1.5-2.5cm) increased number (per plot) and total yield (ton/ha) of marketable bulbs
significantly, and average weight and diameter of single marketable bulbs. The onion cultivar ‘Texas 502’ produced
the highest number (per plot) and total yield (ton/ha) of marketable bulbs, lowest number (per plot) and total yield
(ton/ha) of doubling, split and early bolting bulbs (ton/ha). The onion cultivar ‘Red Amposta’ produced the highest
plant height (cm), no. of leaves/plant, days to maturity, average weight and diameter of single marketable bulbs,
highest number (per plot) and total yield (ton/ha) of doubling and early bolting bulbs. The onion cultivar ‘Giza 6’,
the smallest sizes of sets(0.5-1.5cm) and surface drip irrigation produced the lowest number (per plot) and total yield
(ton/ha) of marketable bulbs. Growing the onion cultivar ‘Texas 502’ using the medium/large sizes of sets (1.5-
3.5cm) and subsurface drip irrigation system significantly increased yield and yield components of marketable
bulbs.
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A field experiment was conducted to study the effect of water regime (W), row spacing (RS) and pla... more A field experiment was conducted to study the effect of water regime (W), row spacing (RS) and plant density (D) on growth
and productivity of tomato cv. ’Pito Pride’ at the Agriculture Experimental Station of King Abdulaziz University. Two water
regimes, W1 (70% of water requirement) and W2 (full water requirement) were studied. Under each water regime three row
spacings (RS1, RS2, RS3) and two plant densities D1 (single plant per dripper) and D2 (two plants per dripper) were investigated.
Results revealed that W1 reduced number of days to flowering and fruit setting, plant height, plant fresh weight and total
yield, while it increased water productivity (WP). Decreasing RS increased water supply and total yield but decreased growth
characteristics. D2 increased total yield by 122–168% and WP by 131–180% compared to D1. Interaction between the three
variables investigated was significant for all assessed characteristics except fruit yield per plant, total fruit yield and WP. These
characteristics were affected by the interaction between RS and D. The highest total yield and WP were obtained from the RS2D2
treatment. This treatment increased total yield per ha by 11–331% and WP by 12–300% compared with the maximum and
minimum yield of other investigated treatments of both seasons. Copyright © 2014 John Wiley & Sons, Ltd.
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ABSTRACT: In order to identify the significance of Na + , Ca + , K + and Mg + concentration and... more ABSTRACT: In order to identify the significance of Na
- , Ca
- , K
- and Mg
- concentration and ratios in plant shoots
and roots as indicators for salinity tolerance, growth and biomass of seven wild, cultivated and inbred lines tomato
genotypes were evaluated under stresses of saline water. The NaCl
was used to prepare four treatments of irrigation
water salinity,2000ppm (3.12 dsm
-1
), 4000ppm (6.25 dsm
-1
2
), 6000ppm (9.37 dsm
-1
) and 8000ppm (12.5 dsm
), while
the control treatment was irrigated with tap water. The experiment was conducted in Completely Randomized design
(CR) using three replications. The results indicated significant differences between tomato genotypes, irrigation
water salinity levels and their interaction at all assessed growth parameters and ions concentration and their ratios in
plant shoots and roots. With increasing the salinity levels, a significant reduction was observed in number of
leaves/plant and plant fresh and dry weight (g) of all tested tomato genotypes. The reduction of growth parameters
observed in ‘LA1421’, ‘KAU I’, ‘KAU II’, ‘F1DOM’ and ‘F1448’ depending on levels of water salinity was found
to be less than those of ‘LA2711’ and ‘F1P#P2’ genotypes. Ions accumulation in plant roots and shoots was
significantly increased with the increase of salinity levels in irrigation water. The accumulation of Na
in
‘LA1421’, ‘F1448’ and ‘F1DOM’ roots and shoots was extremely higher than that accumulated in ‘LA2711’, ‘KAU
I’, ‘KAU II’ and ‘F
1
P#P2’. The K - /Na
- , K
- /Ca
- and Ca
- /Na
- values in plant roots and shoots decreased significantly
with the increase of salinity stresses except K - /Ca
- in ‘KAU I’ shoot and ‘KAU II’ and ‘F
P#P2’ roots. The tomato
genotype ‘KAU I’ and ‘KAU II’ reflected promised genetic stability as it revealed consistent tolerance behavior to
the increase of salinity levels in the irrigation water through the less Na
+,
Ca
+
and Mg - 1
and high K/Na in addition to
the high number of leaves/plant and dry and fresh weight. The reduction in uptake and accumulation of Na
increasing K - uptake, and greater no. of leaves/plant and plant fresh and dry weight are highly recommended as
indicators to salinity tolerance in tomato. - -1
and Ca - and Ca
- ,
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Abstract: Induced androgenesis of cabbage anthers using BAP in combination with NAA and 2,4- D, a... more Abstract: Induced androgenesis of cabbage anthers using BAP in combination with NAA and 2,4- D, and different sucrose concentrations were investigated. The experiment was conducted in 2013 at the lab of plant tissue culture, Department of Arid Land Agriculture, King Abdullaziz University, Saudi Arabia. Anthers at the stage of microsporogenesis of the first formed flower buds and the MS Basel media supplemented with AC (0.5gl-1) were used. The culture medium was supplemented with BAP in combination with NAA and 2,4- D and different sucrose concentrations. The culture medium MS-7 (1mgl-1 BAP + 0.5mgl-1 2, 4- D) enhanced 90% of the cultured anthers to form embryos followed by MS-8 (1mg.l-1 BAP + 1mgl-1 2,4- D) with 89.67%. The MS supplemented with 5mgl-1BAP + 1mgl-1 NAA (MS-2) revented the development of cabbage anthers. The formed embryos were stimulated to develop callus and plantlets on the medium supplemented with lower concentration of BAP and 2,4- D (MS-7 and MS-8). The MS-7 medium improved 49.60% of the formed embryos to develop callus and 27.60% to plantlets. Percentage of embryos that formed callus on MS-8 medium was 51.82% of which 19.87% developed plantlets. Higher concentration of BAP in the culture medium decreased percentage of embr yos producing plantlets. Higher percentage (89 and 90%) of anthers induced embryos and embryos formed plantlets (34.31 and 26.67%) were
stimulated on MS medium with lower sucrose concentration (20gl-1 and 30 gl-1
). Increasing sucrose concentration to 50gl-1 in the culture media enhanced embryos to develop callus and reduced the percentage of embr yos developing
into plantlets.
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Genetic variation for plant breeding, 2004
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Accepted Original Artical for Publication, Jan 2014
Improved Androgenesis of Broccoli (Brassica oleracea var italica) Anthers Using Sucrose and Grow... more Improved Androgenesis of Broccoli (Brassica oleracea var italica) Anthers Using Sucrose and Growth Regulators
Magdi A A Mousa1,2 , Ashraf G. Haridy1, Hassan S Abbass1 and Mohammed F. Mohammed1
1Department of Arid Land Agriculture, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah, Saudi Arabia
2Department of Horticulture, Faculty of Agriculture, Assiut University, 71526 Assiut, Egypt
Abstract
The presented work was conducted to study the effects of BAP and its combinations with 2, 4- D and sucrose concentrations on androgenesis of broccoli cv. ‘Arcadia’ . The MS Basel media supplemented with AC (0.5g/L-1) and 3% sucrose was used. The experiment was laid out in Completely Randomized Design (CRD) using 3 replicates. The effects of BAP in combination with 2,4-D and sucrose concentrations on androgenesis of broccoli (cv Arcadia) anther cultures were investigated. The MS basal media supplemented with activated charcoal (AC) (0.5g.l-1) was used. The medium complemented with BAP (1mg.l-1) plus 2,4-D (0.5 mg.l-1) significantly increased the percentage of anthers developing embryos (90%). Increasing 2,4-D concentration (1mg.l-1) significantly decreased the percentage of anthers formed embryos (10%). Regarding the regeneration of the formed embryos, the culture medium containing BAP (1mg.l-1) + 2,4-D (0.5mg.l-1) or BAP (1mg. .l-1) + 2,4-D (1mg.l-1) produced the high percentages of embryos induced callus. The maximum percentage of embryos developed into plantlets were obtained with culture medium with BAP (1mg.l-1) + 2,4- D (0.5mg.l-1) followed by BAP (1mg.l-1) and 2,4-D (1mg.l-1). Concerning sucrose concentration, the culture medium with low sucrose concentration (20g.l-1) enhanced the percentage of anthers developed embryos. The higher sucrose concentrations (50 and 60g.l-1) increased percentages of dead anthers and anthers formed callus and decreased the percentage of embryos developing plantlets. BAP in combinations with 2,4-D and low concentrations of sucrose can be successfully used to perform embryos and/or callus from the callus induction and embryos development either to callus or plants.
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Magdi Ali Ahmed Mousa and Ahmed Abdullah Said bakhashwain, Mar 1, 2014
The genotypic and BAP and kin in combination with NAA, IAA and 2,4- D effects on in vitro nodal ... more The genotypic and BAP and kin in combination with NAA, IAA and 2,4- D effects on in vitro nodal
segments of jojoba were investigated. The 1.0 - 2.0 cm nodal segments were prepared from the newly established
lateral branches on the 5 years old jojoba plants. Shoot initiation and elongation was obtained using the MS Basel
medium supplemented with 0.5g/l-1
activated charcoal. The genotype "Hada Al-Sham" produced the highest no. of
regenerated shoots, no. of nodes/shoot, length of primary shoots and no. of shoots/explants. Jojoba genotypes
'Medina’ and ‘Hael B’ recorded the least values of shoot formation and regeneration at all tested growth regulator
combination. The culture medium supplemented with high BAP and IAA concentration enhanced shoot formation of
the nodal segments of the four tested jojoba genotypes. The maximum length of regenerated shoots, no. of
nods/shoot and no. of shoots /explants were obtained on the culture medium MS + 10 μM BAP + 5 μM IAA. Callus
formation observed when the nodal segments were cultured on MS + 10 μM BAP + 10 μM NAA and MS + 10 μM
BAP + 10 μM IAA. Nodal segments of the tested genotypes were not stimulated on culture medium supplemented
with BAP in combinations with 2, 4-D and kin alone and its combination with NAA, IAA and 2,4-D.
[Magdi Ali Ahmed Mousa and Ahmed Abduallah Said bakhashwain.
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Theoretical and Applied Genetics, 2009
The beet cyst nematode (Heterodera schachtii Schmidt) can be controlled biologically in highly in... more The beet cyst nematode (Heterodera schachtii Schmidt) can be controlled biologically in highly infected soils of sugar beet rotations using resistant varieties of oil radish (Raphanus sativus L. ssp. oleiferus DC.) as a green crop. Resistant plants stimulate infective juveniles to invade roots, but prevent them after their penetration to complete the life cycle. The resistance trait has been transferred successfully to susceptible rapeseed by the addition of a complete radish chromosome. The aim of the study was to construct a genetic map for radish and to develop resistance-associated markers. The map with 545 RAPD, dpRAPD, AFLP and SSR markers had a length of 1,517 cM, a mean distance of 2.8 cM and consisted of nine linkage groups having sizes between 120 and 232 cM. Chromosome-specific markers for the resistance-bearing chromosome d and the other eight radish chromosomes, developed previously from a series of rapeseed-radish addition lines, were enclosed as anchor markers. Each of the extra chromosomes in the addition lines could be unambiguously assigned to one of the radish linkage groups. The QTL analysis of nematode resistance was realized in the intraspecific F2 mapping population derived from a cross between varieties ‘Pegletta’ (nematode resistant) x ‘Siletta Nova’ (susceptible). A dominant major QTL Hs1Rph explaining 46.4% of the phenotypic variability was detected in a proximal position of chromosome d. Radish chromosome-specific anchor markers with known map positions were made available for future recombination experiments to incorporate segments carrying desired genes as Hs1Rph from radish into rapeseed by means of chromosome addition lines.
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International Journal of Vegetable Science, 2007
Okra (Abelmoschus esculentusL.) cv. ‘Balady’, that is monocropped over 5-6 months in Upper Egypt ... more Okra (Abelmoschus esculentusL.) cv. ‘Balady’, that is monocropped over 5-6 months in Upper Egypt usually grows slowly in the first 5-7 weeks. There is no information available on whether a secondary summer vegetable can be produced within main crop okra rows. In the present study, okra was planted on April 1,2004 and April 7,2005 at 40 cm within-row spaces on 70-cm wide rows. Cowpea or cucumber was planted at mid-distance between okra hills either simultaneously with okra (April 1, 2004 and April 7, 2005) or later after growing okra (April 27, 2004 and April 28, 2005). Sole crop treatments were also involved. Data indicated that okra-cowpea intercropping did not affect okra pod yield. Cowpea, on average, produced 257 kg·ha−1 dry seed yield when planted simultaneously or 3 weeks after planting okra. Calculated “aggressiveness” indicated that okra was the dominant crop. Land equivalent ratio (LER) of okra-cowpea intercropping was, on average, 1.2 for both years. With regard to cucumber, fruit yield was produced only when it was planted simultaneously with okra. Okra-cucumber intercropping based on simultaneous planting of both crops depressed okra pod yield to 90% of its monocropped yield. Intercropped cucumber yield as percent of sole culture was 60%. “Aggressiveness” showed that cucumber was the dominant crop. LER of okra-cucumber intercropping, on average, was 1.5 when both crops were simultaneously planted.
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Books by Magdy Mousa
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Tomato is an important vegetable crop and its production is adversely affected by leaf curl disea... more Tomato is an important vegetable crop and its
production is adversely affected by leaf curl disease caused
by begomovirus. Leaf curl disease is a serious concern for
tomato crops caused by begomovirus in Jeddah, Kingdom
of Saudi Arabia. Tomato leaf curl disease has been shown
to be mainly caused either by tomato leaf curl Sudan virus
or tomato yellow leaf curl virus as well as tomato leaf curl
Oman virus. Many tomato plants infected with monopartite
begomoviruses were also found to harbor a symptom
enhancing betasatellites. Here we report the association of
tomato leaf curl Sudan virus causing leaf curl disease of
tomato in Jeddah, Kingdom of Saudi Arabia. The complete
genome sequence analysis showed highest (99.9 %) identity
with tomato leaf curl Sudan virus causing leaf curl
disease in Arabian Peninsula. In phylogenetic relationships
analysis, the identified virus formed closest cluster with
tomato leaf curl Sudan virus. In recombination analysis
study, the major parent was identified as tomato leaf curl
Sudan virus. Findings of this study strongly supports the
associated virus is a variant of tomato leaf curl Sudan virus
causing disease in Sudan, Yemen and Arabian Peninsula.
The betasatellites sequence analysis showed highest identity
(99.8 %) with tomato leaf curl betasatellites-Amaranthus-Jeddah.
The phylogenetic analysis result based on
betasatellites formed closed cluster with tomato yellow leaf curl Oman betasatellites. The importance of these findings
and occurrence of begomovirus in new geographic regions
causing leaf curl disease of tomato in Jeddah, Kingdom of
Saudi Arabia are discussed.
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Acta Diabetologica, 1999
Type 2 (non-insulin-dependent) diabetes is emerging as a leading chronic non-communicable disease... more Type 2 (non-insulin-dependent) diabetes is emerging as a leading chronic non-communicable disease among the adult Kuwaiti population. Based on the World Health Organization and similar reports the projected estimates for subjects suffering from type 2 diabetes by the years 2000 and 2010 show a striking tendency to high rates of the disease in our area. We report the prevalence rates of glucose intolerance among a relatively young adult Kuwaiti population below 50 years of age and the effect of implementing the recent 1997 American Diabetes Association diagnostic criteria on the frequency of type 2 diabetes, impaired glucose tolerance and impaired fasting glucose among this group. The overall prevalence rate for the three categories of glucose intolerance reached as high as 15.8% (95% CI, 14.2 to 17.4). Age, though all subjects were below 50 years, parental history of type 2 diabetes, diastolic blood pressure and serum triglycerides were found to be significant associated risk factors for the development of type 2 diabetes. Obesity was an apparent significant factor associated with the three forms of glucose intolerance (p < 0.001). Obesity and physical inactivity were documented in both non-diabetic and more so in diabetic Kuwaiti adults, which should form the basis of any immediate intervention programme. An integrated approach to the prevention of the described critical risk factors associated with type 2 diabetes is highly recommended in Kuwait. Research, focused on genetics of type 2 diabetes in the highly susceptible Kuwaiti population, should be planned.
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Tomato (Solanum lycopersicum L.) production is severely affected by many diseases caused by many ... more Tomato (Solanum lycopersicum L.) production is severely affected by many diseases caused by many pathogens and among them viruses are the most serious pathogen. Begomoviruses causes yellow mosaic and leaf curl disease of tomato in the tropical, subtropical, temperate, and even semiarid regions. Yellow leaf curl disease is caused by Tomato yellow leaf curl virus belonging to the genus Begomovirus of the family Geminiviridae. In this study, naturally infected tomato leaf samples were collected during field survey and causal virus was identified by PCR using tomato yellow leaf curl virus-specific primers and transmitted by whiteflies to healthy tomato seedlings. The full-length viral genome was amplified by rolling circle amplification technology while betasatellites from viral genome were amplified by PCR using universal betasatellites primers. The full-length viral genome (~2.7kb) and betasatellites (~1.4kb) were cloned and sequenced bi-directionally. The generated sequences were assembled and analyzed to find out the genetic variability by using bioinformatics tools and the genetic variability and phylogenetic relationships with selected begomoviruses were analyzed. The complete viral genome sequences showed highest (99.5%) similarity with an isolate of Tomato Yellow leaf curl virus-Jizan 103 isolate103 and 92.8% similarity with Tomato Yellow leaf curl virus-Al-Qasim isolate while the lowest (71.6%) were found with Tomato Yellow leaf curl virus-Egypt isolate. The newly identified virus formed the closest cluster with Tomato yellow leaf curl virus isolates from Jizan and Al-Qasim, Saudi Arabia. On the basis of sequence similarity and phylogenetic relationship and recombination analysis, it is concluded that the virus causing tomato yellow leaf curl disease could be a variant of tomato yellow leaf curl virus either from Jizan or Al-Qasim isolate circulating in the Kingdom of Saudi Arabia.
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Abstract: A field experiment was conducted during two cropping seasons 2012 and 2013 at the Agric... more Abstract: A field experiment was conducted during two cropping seasons 2012 and 2013 at the Agriculture
Research Station belonging to King Abdulaziz University to study the effects of set sizes (SS) and irrigation system
(IS) on growth, yield, doubling and early bolting of three onion cultivars. Three sizes of sets, small (0.5-1.5cm),
medium (1.5-2.5cm) and large (2.5-3.5cm) of onion cultivars ‘Red Amposta’, ‘Giza 6’ and ‘Texas
502’werecultivated using surface and subsurface drip irrigation systems. All experiments were laid out in
Randomized Complete Block Design (RCBD) using 3 replicates. The results showed that growth, yield and bulbs
quality of the tested onions cultivars were significantly affected by the irrigation systems and sizes of sets and their
interactions. The surface drip irrigation (IS1) produced the highest plant height and no. of leaves/plant, highest no.
of doubling, split and early bolting bulbs/plot. The subsurface drip irrigation (IS2) produced the highest average
weight and diameter of single marketable bulbs, no. of marketable bulbs/plot, total yield of marketable, doubling,
split and early bolting bulbs (ton/ha). The sizes of sets 2.5-3.5cm (SS3) significantly increased plant height (cm), no.
of leaves/plant, days to maturity, number (per plot) and total yield (ton/ha) of doubling, split and early bolting bulbs.
Using the medium sizes of sets(1.5-2.5cm) increased number (per plot) and total yield (ton/ha) of marketable bulbs
significantly, and average weight and diameter of single marketable bulbs. The onion cultivar ‘Texas 502’ produced
the highest number (per plot) and total yield (ton/ha) of marketable bulbs, lowest number (per plot) and total yield
(ton/ha) of doubling, split and early bolting bulbs (ton/ha). The onion cultivar ‘Red Amposta’ produced the highest
plant height (cm), no. of leaves/plant, days to maturity, average weight and diameter of single marketable bulbs,
highest number (per plot) and total yield (ton/ha) of doubling and early bolting bulbs. The onion cultivar ‘Giza 6’,
the smallest sizes of sets(0.5-1.5cm) and surface drip irrigation produced the lowest number (per plot) and total yield
(ton/ha) of marketable bulbs. Growing the onion cultivar ‘Texas 502’ using the medium/large sizes of sets (1.5-
3.5cm) and subsurface drip irrigation system significantly increased yield and yield components of marketable
bulbs.
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A field experiment was conducted to study the effect of water regime (W), row spacing (RS) and pla... more A field experiment was conducted to study the effect of water regime (W), row spacing (RS) and plant density (D) on growth
and productivity of tomato cv. ’Pito Pride’ at the Agriculture Experimental Station of King Abdulaziz University. Two water
regimes, W1 (70% of water requirement) and W2 (full water requirement) were studied. Under each water regime three row
spacings (RS1, RS2, RS3) and two plant densities D1 (single plant per dripper) and D2 (two plants per dripper) were investigated.
Results revealed that W1 reduced number of days to flowering and fruit setting, plant height, plant fresh weight and total
yield, while it increased water productivity (WP). Decreasing RS increased water supply and total yield but decreased growth
characteristics. D2 increased total yield by 122–168% and WP by 131–180% compared to D1. Interaction between the three
variables investigated was significant for all assessed characteristics except fruit yield per plant, total fruit yield and WP. These
characteristics were affected by the interaction between RS and D. The highest total yield and WP were obtained from the RS2D2
treatment. This treatment increased total yield per ha by 11–331% and WP by 12–300% compared with the maximum and
minimum yield of other investigated treatments of both seasons. Copyright © 2014 John Wiley & Sons, Ltd.
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ABSTRACT: In order to identify the significance of Na + , Ca + , K + and Mg + concentration and... more ABSTRACT: In order to identify the significance of Na
- , Ca
- , K
- and Mg
- concentration and ratios in plant shoots
and roots as indicators for salinity tolerance, growth and biomass of seven wild, cultivated and inbred lines tomato
genotypes were evaluated under stresses of saline water. The NaCl
was used to prepare four treatments of irrigation
water salinity,2000ppm (3.12 dsm
-1
), 4000ppm (6.25 dsm
-1
2
), 6000ppm (9.37 dsm
-1
) and 8000ppm (12.5 dsm
), while
the control treatment was irrigated with tap water. The experiment was conducted in Completely Randomized design
(CR) using three replications. The results indicated significant differences between tomato genotypes, irrigation
water salinity levels and their interaction at all assessed growth parameters and ions concentration and their ratios in
plant shoots and roots. With increasing the salinity levels, a significant reduction was observed in number of
leaves/plant and plant fresh and dry weight (g) of all tested tomato genotypes. The reduction of growth parameters
observed in ‘LA1421’, ‘KAU I’, ‘KAU II’, ‘F1DOM’ and ‘F1448’ depending on levels of water salinity was found
to be less than those of ‘LA2711’ and ‘F1P#P2’ genotypes. Ions accumulation in plant roots and shoots was
significantly increased with the increase of salinity levels in irrigation water. The accumulation of Na
in
‘LA1421’, ‘F1448’ and ‘F1DOM’ roots and shoots was extremely higher than that accumulated in ‘LA2711’, ‘KAU
I’, ‘KAU II’ and ‘F
1
P#P2’. The K - /Na
- , K
- /Ca
- and Ca
- /Na
- values in plant roots and shoots decreased significantly
with the increase of salinity stresses except K - /Ca
- in ‘KAU I’ shoot and ‘KAU II’ and ‘F
P#P2’ roots. The tomato
genotype ‘KAU I’ and ‘KAU II’ reflected promised genetic stability as it revealed consistent tolerance behavior to
the increase of salinity levels in the irrigation water through the less Na
+,
Ca
+
and Mg - 1
and high K/Na in addition to
the high number of leaves/plant and dry and fresh weight. The reduction in uptake and accumulation of Na
increasing K - uptake, and greater no. of leaves/plant and plant fresh and dry weight are highly recommended as
indicators to salinity tolerance in tomato. - -1
and Ca - and Ca
- ,
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Abstract: Induced androgenesis of cabbage anthers using BAP in combination with NAA and 2,4- D, a... more Abstract: Induced androgenesis of cabbage anthers using BAP in combination with NAA and 2,4- D, and different sucrose concentrations were investigated. The experiment was conducted in 2013 at the lab of plant tissue culture, Department of Arid Land Agriculture, King Abdullaziz University, Saudi Arabia. Anthers at the stage of microsporogenesis of the first formed flower buds and the MS Basel media supplemented with AC (0.5gl-1) were used. The culture medium was supplemented with BAP in combination with NAA and 2,4- D and different sucrose concentrations. The culture medium MS-7 (1mgl-1 BAP + 0.5mgl-1 2, 4- D) enhanced 90% of the cultured anthers to form embryos followed by MS-8 (1mg.l-1 BAP + 1mgl-1 2,4- D) with 89.67%. The MS supplemented with 5mgl-1BAP + 1mgl-1 NAA (MS-2) revented the development of cabbage anthers. The formed embryos were stimulated to develop callus and plantlets on the medium supplemented with lower concentration of BAP and 2,4- D (MS-7 and MS-8). The MS-7 medium improved 49.60% of the formed embryos to develop callus and 27.60% to plantlets. Percentage of embryos that formed callus on MS-8 medium was 51.82% of which 19.87% developed plantlets. Higher concentration of BAP in the culture medium decreased percentage of embr yos producing plantlets. Higher percentage (89 and 90%) of anthers induced embryos and embryos formed plantlets (34.31 and 26.67%) were
stimulated on MS medium with lower sucrose concentration (20gl-1 and 30 gl-1
). Increasing sucrose concentration to 50gl-1 in the culture media enhanced embryos to develop callus and reduced the percentage of embr yos developing
into plantlets.
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Genetic variation for plant breeding, 2004
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Accepted Original Artical for Publication, Jan 2014
Improved Androgenesis of Broccoli (Brassica oleracea var italica) Anthers Using Sucrose and Grow... more Improved Androgenesis of Broccoli (Brassica oleracea var italica) Anthers Using Sucrose and Growth Regulators
Magdi A A Mousa1,2 , Ashraf G. Haridy1, Hassan S Abbass1 and Mohammed F. Mohammed1
1Department of Arid Land Agriculture, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah, Saudi Arabia
2Department of Horticulture, Faculty of Agriculture, Assiut University, 71526 Assiut, Egypt
Abstract
The presented work was conducted to study the effects of BAP and its combinations with 2, 4- D and sucrose concentrations on androgenesis of broccoli cv. ‘Arcadia’ . The MS Basel media supplemented with AC (0.5g/L-1) and 3% sucrose was used. The experiment was laid out in Completely Randomized Design (CRD) using 3 replicates. The effects of BAP in combination with 2,4-D and sucrose concentrations on androgenesis of broccoli (cv Arcadia) anther cultures were investigated. The MS basal media supplemented with activated charcoal (AC) (0.5g.l-1) was used. The medium complemented with BAP (1mg.l-1) plus 2,4-D (0.5 mg.l-1) significantly increased the percentage of anthers developing embryos (90%). Increasing 2,4-D concentration (1mg.l-1) significantly decreased the percentage of anthers formed embryos (10%). Regarding the regeneration of the formed embryos, the culture medium containing BAP (1mg.l-1) + 2,4-D (0.5mg.l-1) or BAP (1mg. .l-1) + 2,4-D (1mg.l-1) produced the high percentages of embryos induced callus. The maximum percentage of embryos developed into plantlets were obtained with culture medium with BAP (1mg.l-1) + 2,4- D (0.5mg.l-1) followed by BAP (1mg.l-1) and 2,4-D (1mg.l-1). Concerning sucrose concentration, the culture medium with low sucrose concentration (20g.l-1) enhanced the percentage of anthers developed embryos. The higher sucrose concentrations (50 and 60g.l-1) increased percentages of dead anthers and anthers formed callus and decreased the percentage of embryos developing plantlets. BAP in combinations with 2,4-D and low concentrations of sucrose can be successfully used to perform embryos and/or callus from the callus induction and embryos development either to callus or plants.
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Magdi Ali Ahmed Mousa and Ahmed Abdullah Said bakhashwain, Mar 1, 2014
The genotypic and BAP and kin in combination with NAA, IAA and 2,4- D effects on in vitro nodal ... more The genotypic and BAP and kin in combination with NAA, IAA and 2,4- D effects on in vitro nodal
segments of jojoba were investigated. The 1.0 - 2.0 cm nodal segments were prepared from the newly established
lateral branches on the 5 years old jojoba plants. Shoot initiation and elongation was obtained using the MS Basel
medium supplemented with 0.5g/l-1
activated charcoal. The genotype "Hada Al-Sham" produced the highest no. of
regenerated shoots, no. of nodes/shoot, length of primary shoots and no. of shoots/explants. Jojoba genotypes
'Medina’ and ‘Hael B’ recorded the least values of shoot formation and regeneration at all tested growth regulator
combination. The culture medium supplemented with high BAP and IAA concentration enhanced shoot formation of
the nodal segments of the four tested jojoba genotypes. The maximum length of regenerated shoots, no. of
nods/shoot and no. of shoots /explants were obtained on the culture medium MS + 10 μM BAP + 5 μM IAA. Callus
formation observed when the nodal segments were cultured on MS + 10 μM BAP + 10 μM NAA and MS + 10 μM
BAP + 10 μM IAA. Nodal segments of the tested genotypes were not stimulated on culture medium supplemented
with BAP in combinations with 2, 4-D and kin alone and its combination with NAA, IAA and 2,4-D.
[Magdi Ali Ahmed Mousa and Ahmed Abduallah Said bakhashwain.
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Theoretical and Applied Genetics, 2009
The beet cyst nematode (Heterodera schachtii Schmidt) can be controlled biologically in highly in... more The beet cyst nematode (Heterodera schachtii Schmidt) can be controlled biologically in highly infected soils of sugar beet rotations using resistant varieties of oil radish (Raphanus sativus L. ssp. oleiferus DC.) as a green crop. Resistant plants stimulate infective juveniles to invade roots, but prevent them after their penetration to complete the life cycle. The resistance trait has been transferred successfully to susceptible rapeseed by the addition of a complete radish chromosome. The aim of the study was to construct a genetic map for radish and to develop resistance-associated markers. The map with 545 RAPD, dpRAPD, AFLP and SSR markers had a length of 1,517 cM, a mean distance of 2.8 cM and consisted of nine linkage groups having sizes between 120 and 232 cM. Chromosome-specific markers for the resistance-bearing chromosome d and the other eight radish chromosomes, developed previously from a series of rapeseed-radish addition lines, were enclosed as anchor markers. Each of the extra chromosomes in the addition lines could be unambiguously assigned to one of the radish linkage groups. The QTL analysis of nematode resistance was realized in the intraspecific F2 mapping population derived from a cross between varieties ‘Pegletta’ (nematode resistant) x ‘Siletta Nova’ (susceptible). A dominant major QTL Hs1Rph explaining 46.4% of the phenotypic variability was detected in a proximal position of chromosome d. Radish chromosome-specific anchor markers with known map positions were made available for future recombination experiments to incorporate segments carrying desired genes as Hs1Rph from radish into rapeseed by means of chromosome addition lines.
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International Journal of Vegetable Science, 2007
Okra (Abelmoschus esculentusL.) cv. ‘Balady’, that is monocropped over 5-6 months in Upper Egypt ... more Okra (Abelmoschus esculentusL.) cv. ‘Balady’, that is monocropped over 5-6 months in Upper Egypt usually grows slowly in the first 5-7 weeks. There is no information available on whether a secondary summer vegetable can be produced within main crop okra rows. In the present study, okra was planted on April 1,2004 and April 7,2005 at 40 cm within-row spaces on 70-cm wide rows. Cowpea or cucumber was planted at mid-distance between okra hills either simultaneously with okra (April 1, 2004 and April 7, 2005) or later after growing okra (April 27, 2004 and April 28, 2005). Sole crop treatments were also involved. Data indicated that okra-cowpea intercropping did not affect okra pod yield. Cowpea, on average, produced 257 kg·ha−1 dry seed yield when planted simultaneously or 3 weeks after planting okra. Calculated “aggressiveness” indicated that okra was the dominant crop. Land equivalent ratio (LER) of okra-cowpea intercropping was, on average, 1.2 for both years. With regard to cucumber, fruit yield was produced only when it was planted simultaneously with okra. Okra-cucumber intercropping based on simultaneous planting of both crops depressed okra pod yield to 90% of its monocropped yield. Intercropped cucumber yield as percent of sole culture was 60%. “Aggressiveness” showed that cucumber was the dominant crop. LER of okra-cucumber intercropping, on average, was 1.5 when both crops were simultaneously planted.
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