Mohsin Shafi | Khyber Medical College Peshawar (original) (raw)

Papers by Mohsin Shafi

Journal of Medical Sciences

Objective: CA 19-9 is a marker that shows the relapse of tumors. Raised CA 19-9 in diabetes melli... more Objective: CA 19-9 is a marker that shows the relapse of tumors. Raised CA 19-9 in diabetes mellitus cases and the effect of hypoglycemic agents on the marker prompted us to compare the effect of metformin and glibenclamide on CA 19-9 levels in patients with diabetes mellitus. Methods: It is a randomized control trial (RCT). Normal control (group A1) included 40 staff members from KTH/KMC. A total number of 79 patients with type 2 diabetes mellitus were randomized into two groups; group A2 (n=39) who took metformin (500 mg/day) and group A3 (n=40) received glibenclamide (2.5 mg/day). The study was conducted for 6 months and variables including fasting blood glucose, blood lipids, plasma sialic acid, HbA1-c, insulin and C-peptide, CA 19-9, and insulin resistance were measured initially and at week 10. Results: At the endpoint, there were reductions in FBG and HbA1C levels among metformin and glibenclamide groups compared to baseline (p<0.003, p<0.001, and p<0.05, p?0.05 res...

Journal of Medical Sciences, 2021

Objectives: To determine the level of depression , anxiety and stresss in pathologists due to Cov... more Objectives: To determine the level of depression , anxiety and stresss in pathologists due to Covid -19 pandemic . Materials and methods: It was a Cross sectional Analytical Multicenter Questionnaire based study conducted from April 2020 to June 2020 by including Pathologists working in Khyber Teaching Hospital and Pakistan Institute of Medical Sciences , Islamabad. Informed written consent was obtained and DASS-21 Questionnaire was administred .Data was analysed by SPSS. Levels of depression, anxiety and stress were determined and analysed by multiple regression to predict depression anxiety and stress levels from demographics. Results: Mean age of the study sample was 25-63 (37±8.75).There were 13(22.4%) males and 45(77.6%) females. Overall DAS score, mean depression , anxiety and stress score were 31.5±22, 8.7±9.1, 7.4±7.1 and 15.3±9 respectively. Females had higher overall DAS score (U=156.5, p=.011), depression score (U=178.5, p=.032) and anxiety score (U=168.5, p=.029) as comp...

International Journal of Pathology, 2019

Objective High molecular weight adiponectin (HMW ADPN) plays an important role in the regulation ... more Objective High molecular weight adiponectin (HMW ADPN) plays an important role in the regulation of insulin resistance and atherogenic processes. However, the role of HMW ADPN remains to be determined in hemodialysis (HD) patients. Patients and Methods We measured serum HMW ADPN in 49 HD patients (age: 62.4±12.0 years, time on HD: 8.4±6.4 years, male/female=30/19), and examined the association between HMW ADPN and visceral fat area (VFA) estimated by abdominal CT scans. Results Serum HMW ADPN concentrations were weakly and inversely correlated with serum TG (r=-0.271, p=0.0598), but significantly and positively correlated with HDL cholesterol (r=0.392, p=0.0050). Serum HMW ADPN levels were positively correlated with BMI (r=0.472, p=0.0084) in male patients, and the HMW ADPN levels were positively correlated with serum levels of HDL cholesterol (r=0.514, p=0.0243) and TG (r=0.605, p=0.0061).The regression coefficient between VFA and HMW ADPN was-0.491 (p< 0.003). Multiple stepwise regression analyses showed that VFA was the most significant and independent determinant of serum HMW ADPN concentration. Conclusion These findings suggest that HMW ADPN may be inversely associated with visceral fat accumulation in HD patients.

1 Department of Pathology, Khyber Medical College, Peshawar, Pakistan Address for correspondence:... more 1 Department of Pathology, Khyber Medical College, Peshawar, Pakistan Address for correspondence: Dr. Haroon Ur Rasheed Assistant Professor Department of Pathology, Khyber Medical College, Peshawar, Pakistan Cell # 0092-3459128455 INTRODUCTION Objective: To study the role of Adenosine deaminase enzyme (ADA) in differentiating tuberculous pleural effusion from malignant pleural effusion. Material and Methods: It was a cross sectional descriptive study carried out in Pulmonology departments of Lady Reading Hospital, Khyber teaching Hospital Peshawar and department of Pathology, Khyber Medical College, Peshawar from April 2015 to Jan 2016. A total of 128 patients including 88 tuberculous and 40 malignant pleural effusion patients were selected through consecutive non-probability sampling technique. Results: In our study, mean ADA levels in pleural fluid were significantly higher for tuberculous than for malignant pleural effusions (p<0.001 ).Pleural fluid ADA levels has sensitivity,...

Introduction: Calcium is the most abundant mineral and fifth most common element in the body. The... more Introduction: Calcium is the most abundant mineral and fifth most common element in the body. There are many different methods for estimation of serum calcium like spectrophotometeric, ion selective electrode (ISE) and atomic absorption methods. Objective: Aim of our study was to compare serum calcium estimation by using direct colorimetric and volume / volume colorimeteric (v/v) methods based on o-Cresolphtalein principle. Materials and Methods: The study was performed in a tertiary care laboratory in Rawalpindi from March to June ‘2011. It was a comparative prospective study. Seventy commercial quality control samples of Randox laboratories were simultaneously tested by both the methods. Results were analyzed on SPSS version 17. Results: In normal controls, the v/v method kit gave the mean result of 2.34 mmol/L ± 0.04 with a CV of 1.70% while with the direct colorimetric method kit the values were 2.31 mmol/L ± 0.03 with a CV of 1.30%. In abnormal controls, v/v method kit gave the...

Introduction: Hypothyroidism is a very common endocrine problem. It can be either overt or subcli... more Introduction: Hypothyroidism is a very common endocrine problem. It can be either overt or subclinical. Subclinical hypothyroidism predisposes to overt hypothyroidism. Hypothyroidism leads to hypercholesterolaemia because of reduced activity of lipoprotein lipase and thus increases the cardiovascular risk. The objective of this study was carried out to find the lipid profile in hypothyroid patients in asymptomatic previously undiagnosed cases and to correlate different components of lipid profile with TSH and fT 4. Materials and Methods: This is a case control study consisting of 82 hypothyroid patients with age and gender matched controls selected through convenient sampling from Chemical Pathology Department, Army Medical College, Rawalpindi and National University of Sciences and Technology (NUST), Islamabad between May and October 2012. Data were recorded using specially designed pro forma and analysis was carried out on SPSS 17. Results: Among the 82 hypothyroid patients, 62 we...

Background: Past research studies have shown a significant correlation between serum leptin with ... more Background: Past research studies have shown a significant correlation between serum leptin with various components of metabolic syndrome. Objective: To establish the correlation of leptin levels with various components of metabolic syndrome, its levels were first measured and then its possible correlation was found out with each component of metabolic syndrome. Methodology: This case-control study was conducted on 100 subjects (50 patients of MetS along with equal number of age and sex matched controls) in Pathology Department, Army medical college, Military Hospital, Rawalpindi. Patients with a history of thyroid, hematological, liver and neoplastic diseases were excluded from this study. After getting information regarding the history, anthropometric indices of obesity were measured and required laboratory investigations were carried out to determine any correlation between serum leptin with metabolic syndrome components. Data was analyzed by using SPSS version 17. Results: Labor...

considerable impact on individuals, families and health and social care services. Objective: To d... more considerable impact on individuals, families and health and social care services. Objective: To determine the frequency of hyperglycemia in patients presenting with acute stroke. Material & Methods: This is across sectional, descriptive study conducted at Khyber Teaching Hospital from January 2017 to January 2018 .A total of 143 patients (male and female ) with acute stroke within 48 hours of stroke onset, aged 45 and above were included.In this study blood samples were obtained immediately after admission and sent to laboratory for blood glucose level. Mean + standard deviation was calculated for quantitative variables like age, duration of stoke and blood glucose levels. Frequency/percentages were calculated for qualitative variables i.e. gender, type of stroke and hyperglycemia. Results: Our study shows that mean age was 63 years with standard deviation ± 28.34. Fifty two percent patients were male and 48% patients were female. The incidence of hyperglycemia in acute stroke was 2...

Journal of Rawalpindi Medical College, 2020

Objective: To study the diagnostic accuracy of Adenosine deaminase enzyme (ADA) in the diagnosis ... more Objective: To study the diagnostic accuracy of Adenosine deaminase enzyme (ADA) in the diagnosis of tuberculous pleural effusion (TPE).Material and Methods: It was a cross-sectional descriptive study conducted in the Pulmonology departments of Lady Reading and Khyber Teaching Hospital Peshawar and department of Pathology, Khyber Medical College, Peshawar from April 2015 to Jan 2016. A total of 210 tuberculous and non-tuberculous pleural effusion patients were selected through consecutive non-probability sampling techniques. After physical and systemic examination, 3cc of pleural fluid was taken. ADA was estimated by Non-Guisti and Galanti method through the simple colorimetric method. All the data was entered in a specially designed proforma and SPSS v16 was used for statistical analysis.Results: Out of 210 tuberculous and non-tuberculous pleural effusions, the commonest cause of pleural effusion was tuberculosis followed by malignancy. In our study, Pleural fluid ADA levels have se...

Biomedica, 2011

Introduction: Dyslipidaemia and hypertension are important predisposing factors leading to cardio... more Introduction: Dyslipidaemia and hypertension are important predisposing factors leading to cardiovascular diseases. Obesity also increases the risk of hypertension, coronary artery disease and dyslipidaemia. Objective: Purpose of this study was to find out a relationship between body mass index and total cholesterol levels in 100 hypertensive patients. Materials and Methods: Study was performed in Chemical Pathology Department of Army Medical College, Rawalpindi in collaboration with Department of Medicine, Combined Military Hospital, Rawalpindi. It is a cross-sectional study and simple non-probability random sampling was used. Hundred hypertensive patients were included in the study. Data was recorded using a detailed proforma and analysis was done on SPSS version 17. Results: Of the 100 hypertensive patients included in the study, there were 39 males while 61 were females. The mean age was 60.1 ± 11.5 years. The mean blood pressure was 171 ± 13.1/105.2 ± 6.6 mm of Hg. Mean body mass index was 28.6 ± 3.97. Mean of total cholesterol was 5.7 ± 0.7 mmol/L in all the patients. Conclusion: Hypertension, dyslipidaemia and increased BMI increase go hand in hand with the risk of cardiovascular disease. Treatment strategies should be adopted to treat hypertension and dyslipidemia aggressively and lifestyle modifications are necessary for reducing BMI.

Diagnosis, 2021

Objectives Antigen based rapid diagnostic tests possesses a potential to be utilized along with G... more Objectives Antigen based rapid diagnostic tests possesses a potential to be utilized along with Gold standard methods to detect Covid-19 infection to cope with the demand of testing. The aim of this study was to determine diagnostic accuracy of electrochemiluminescence based automated antigen detection immunoassay comparing with molecular based test RT-PCR (Covid-19). Methods It was a cross-sectional study conducted in RMI Peshawar, from 1st April 2021 till 30th April 2021. The study comprised 170 individuals who were suspected of having Covid-19. Nasopharyngeal samples taken from suspected individuals were analyzed by RT-PCR and automated antigen test (Elecsys SARS-CoV-2 Antigen) simultaneously. The correlation of SARS-CoV-2 antigen with PCR positive and negative cases was analyzed for specificity, sensitivity respectively. Results The ECLIA based Elecsys antigen test (Roche) revealed overall sensitivity 72%, specificity 95% and accuracy of 94.9%. Sensitivity of antigen test progre...

Journal of Medical Sciences

Objective: CA 19-9 is a marker that shows the relapse of tumors. Raised CA 19-9 in diabetes melli... more Objective: CA 19-9 is a marker that shows the relapse of tumors. Raised CA 19-9 in diabetes mellitus cases and the effect of hypoglycemic agents on the marker prompted us to compare the effect of metformin and glibenclamide on CA 19-9 levels in patients with diabetes mellitus. Methods: It is a randomized control trial (RCT). Normal control (group A1) included 40 staff members from KTH/KMC. A total number of 79 patients with type 2 diabetes mellitus were randomized into two groups; group A2 (n=39) who took metformin (500 mg/day) and group A3 (n=40) received glibenclamide (2.5 mg/day). The study was conducted for 6 months and variables including fasting blood glucose, blood lipids, plasma sialic acid, HbA1-c, insulin and C-peptide, CA 19-9, and insulin resistance were measured initially and at week 10. Results: At the endpoint, there were reductions in FBG and HbA1C levels among metformin and glibenclamide groups compared to baseline (p<0.003, p<0.001, and p<0.05, p?0.05 res...

Journal of Medical Sciences, 2021

Objectives: To determine the level of depression , anxiety and stresss in pathologists due to Cov... more Objectives: To determine the level of depression , anxiety and stresss in pathologists due to Covid -19 pandemic . Materials and methods: It was a Cross sectional Analytical Multicenter Questionnaire based study conducted from April 2020 to June 2020 by including Pathologists working in Khyber Teaching Hospital and Pakistan Institute of Medical Sciences , Islamabad. Informed written consent was obtained and DASS-21 Questionnaire was administred .Data was analysed by SPSS. Levels of depression, anxiety and stress were determined and analysed by multiple regression to predict depression anxiety and stress levels from demographics. Results: Mean age of the study sample was 25-63 (37±8.75).There were 13(22.4%) males and 45(77.6%) females. Overall DAS score, mean depression , anxiety and stress score were 31.5±22, 8.7±9.1, 7.4±7.1 and 15.3±9 respectively. Females had higher overall DAS score (U=156.5, p=.011), depression score (U=178.5, p=.032) and anxiety score (U=168.5, p=.029) as comp...

International Journal of Pathology, 2019

Objective High molecular weight adiponectin (HMW ADPN) plays an important role in the regulation ... more Objective High molecular weight adiponectin (HMW ADPN) plays an important role in the regulation of insulin resistance and atherogenic processes. However, the role of HMW ADPN remains to be determined in hemodialysis (HD) patients. Patients and Methods We measured serum HMW ADPN in 49 HD patients (age: 62.4±12.0 years, time on HD: 8.4±6.4 years, male/female=30/19), and examined the association between HMW ADPN and visceral fat area (VFA) estimated by abdominal CT scans. Results Serum HMW ADPN concentrations were weakly and inversely correlated with serum TG (r=-0.271, p=0.0598), but significantly and positively correlated with HDL cholesterol (r=0.392, p=0.0050). Serum HMW ADPN levels were positively correlated with BMI (r=0.472, p=0.0084) in male patients, and the HMW ADPN levels were positively correlated with serum levels of HDL cholesterol (r=0.514, p=0.0243) and TG (r=0.605, p=0.0061).The regression coefficient between VFA and HMW ADPN was-0.491 (p< 0.003). Multiple stepwise regression analyses showed that VFA was the most significant and independent determinant of serum HMW ADPN concentration. Conclusion These findings suggest that HMW ADPN may be inversely associated with visceral fat accumulation in HD patients.

1 Department of Pathology, Khyber Medical College, Peshawar, Pakistan Address for correspondence:... more 1 Department of Pathology, Khyber Medical College, Peshawar, Pakistan Address for correspondence: Dr. Haroon Ur Rasheed Assistant Professor Department of Pathology, Khyber Medical College, Peshawar, Pakistan Cell # 0092-3459128455 INTRODUCTION Objective: To study the role of Adenosine deaminase enzyme (ADA) in differentiating tuberculous pleural effusion from malignant pleural effusion. Material and Methods: It was a cross sectional descriptive study carried out in Pulmonology departments of Lady Reading Hospital, Khyber teaching Hospital Peshawar and department of Pathology, Khyber Medical College, Peshawar from April 2015 to Jan 2016. A total of 128 patients including 88 tuberculous and 40 malignant pleural effusion patients were selected through consecutive non-probability sampling technique. Results: In our study, mean ADA levels in pleural fluid were significantly higher for tuberculous than for malignant pleural effusions (p<0.001 ).Pleural fluid ADA levels has sensitivity,...

Introduction: Calcium is the most abundant mineral and fifth most common element in the body. The... more Introduction: Calcium is the most abundant mineral and fifth most common element in the body. There are many different methods for estimation of serum calcium like spectrophotometeric, ion selective electrode (ISE) and atomic absorption methods. Objective: Aim of our study was to compare serum calcium estimation by using direct colorimetric and volume / volume colorimeteric (v/v) methods based on o-Cresolphtalein principle. Materials and Methods: The study was performed in a tertiary care laboratory in Rawalpindi from March to June ‘2011. It was a comparative prospective study. Seventy commercial quality control samples of Randox laboratories were simultaneously tested by both the methods. Results were analyzed on SPSS version 17. Results: In normal controls, the v/v method kit gave the mean result of 2.34 mmol/L ± 0.04 with a CV of 1.70% while with the direct colorimetric method kit the values were 2.31 mmol/L ± 0.03 with a CV of 1.30%. In abnormal controls, v/v method kit gave the...

Introduction: Hypothyroidism is a very common endocrine problem. It can be either overt or subcli... more Introduction: Hypothyroidism is a very common endocrine problem. It can be either overt or subclinical. Subclinical hypothyroidism predisposes to overt hypothyroidism. Hypothyroidism leads to hypercholesterolaemia because of reduced activity of lipoprotein lipase and thus increases the cardiovascular risk. The objective of this study was carried out to find the lipid profile in hypothyroid patients in asymptomatic previously undiagnosed cases and to correlate different components of lipid profile with TSH and fT 4. Materials and Methods: This is a case control study consisting of 82 hypothyroid patients with age and gender matched controls selected through convenient sampling from Chemical Pathology Department, Army Medical College, Rawalpindi and National University of Sciences and Technology (NUST), Islamabad between May and October 2012. Data were recorded using specially designed pro forma and analysis was carried out on SPSS 17. Results: Among the 82 hypothyroid patients, 62 we...

Background: Past research studies have shown a significant correlation between serum leptin with ... more Background: Past research studies have shown a significant correlation between serum leptin with various components of metabolic syndrome. Objective: To establish the correlation of leptin levels with various components of metabolic syndrome, its levels were first measured and then its possible correlation was found out with each component of metabolic syndrome. Methodology: This case-control study was conducted on 100 subjects (50 patients of MetS along with equal number of age and sex matched controls) in Pathology Department, Army medical college, Military Hospital, Rawalpindi. Patients with a history of thyroid, hematological, liver and neoplastic diseases were excluded from this study. After getting information regarding the history, anthropometric indices of obesity were measured and required laboratory investigations were carried out to determine any correlation between serum leptin with metabolic syndrome components. Data was analyzed by using SPSS version 17. Results: Labor...

considerable impact on individuals, families and health and social care services. Objective: To d... more considerable impact on individuals, families and health and social care services. Objective: To determine the frequency of hyperglycemia in patients presenting with acute stroke. Material & Methods: This is across sectional, descriptive study conducted at Khyber Teaching Hospital from January 2017 to January 2018 .A total of 143 patients (male and female ) with acute stroke within 48 hours of stroke onset, aged 45 and above were included.In this study blood samples were obtained immediately after admission and sent to laboratory for blood glucose level. Mean + standard deviation was calculated for quantitative variables like age, duration of stoke and blood glucose levels. Frequency/percentages were calculated for qualitative variables i.e. gender, type of stroke and hyperglycemia. Results: Our study shows that mean age was 63 years with standard deviation ± 28.34. Fifty two percent patients were male and 48% patients were female. The incidence of hyperglycemia in acute stroke was 2...

Journal of Rawalpindi Medical College, 2020

Objective: To study the diagnostic accuracy of Adenosine deaminase enzyme (ADA) in the diagnosis ... more Objective: To study the diagnostic accuracy of Adenosine deaminase enzyme (ADA) in the diagnosis of tuberculous pleural effusion (TPE).Material and Methods: It was a cross-sectional descriptive study conducted in the Pulmonology departments of Lady Reading and Khyber Teaching Hospital Peshawar and department of Pathology, Khyber Medical College, Peshawar from April 2015 to Jan 2016. A total of 210 tuberculous and non-tuberculous pleural effusion patients were selected through consecutive non-probability sampling techniques. After physical and systemic examination, 3cc of pleural fluid was taken. ADA was estimated by Non-Guisti and Galanti method through the simple colorimetric method. All the data was entered in a specially designed proforma and SPSS v16 was used for statistical analysis.Results: Out of 210 tuberculous and non-tuberculous pleural effusions, the commonest cause of pleural effusion was tuberculosis followed by malignancy. In our study, Pleural fluid ADA levels have se...

Biomedica, 2011

Introduction: Dyslipidaemia and hypertension are important predisposing factors leading to cardio... more Introduction: Dyslipidaemia and hypertension are important predisposing factors leading to cardiovascular diseases. Obesity also increases the risk of hypertension, coronary artery disease and dyslipidaemia. Objective: Purpose of this study was to find out a relationship between body mass index and total cholesterol levels in 100 hypertensive patients. Materials and Methods: Study was performed in Chemical Pathology Department of Army Medical College, Rawalpindi in collaboration with Department of Medicine, Combined Military Hospital, Rawalpindi. It is a cross-sectional study and simple non-probability random sampling was used. Hundred hypertensive patients were included in the study. Data was recorded using a detailed proforma and analysis was done on SPSS version 17. Results: Of the 100 hypertensive patients included in the study, there were 39 males while 61 were females. The mean age was 60.1 ± 11.5 years. The mean blood pressure was 171 ± 13.1/105.2 ± 6.6 mm of Hg. Mean body mass index was 28.6 ± 3.97. Mean of total cholesterol was 5.7 ± 0.7 mmol/L in all the patients. Conclusion: Hypertension, dyslipidaemia and increased BMI increase go hand in hand with the risk of cardiovascular disease. Treatment strategies should be adopted to treat hypertension and dyslipidemia aggressively and lifestyle modifications are necessary for reducing BMI.

Diagnosis, 2021

Objectives Antigen based rapid diagnostic tests possesses a potential to be utilized along with G... more Objectives Antigen based rapid diagnostic tests possesses a potential to be utilized along with Gold standard methods to detect Covid-19 infection to cope with the demand of testing. The aim of this study was to determine diagnostic accuracy of electrochemiluminescence based automated antigen detection immunoassay comparing with molecular based test RT-PCR (Covid-19). Methods It was a cross-sectional study conducted in RMI Peshawar, from 1st April 2021 till 30th April 2021. The study comprised 170 individuals who were suspected of having Covid-19. Nasopharyngeal samples taken from suspected individuals were analyzed by RT-PCR and automated antigen test (Elecsys SARS-CoV-2 Antigen) simultaneously. The correlation of SARS-CoV-2 antigen with PCR positive and negative cases was analyzed for specificity, sensitivity respectively. Results The ECLIA based Elecsys antigen test (Roche) revealed overall sensitivity 72%, specificity 95% and accuracy of 94.9%. Sensitivity of antigen test progre...