Ayman E El-Sharkawey | Kuwait University (original) (raw)

Papers by Ayman E El-Sharkawey

Australian Journal of Basic and Applied Sciences

The effectiveness of several antioxidant materials in protecting Bacillus thuringiensis var. kurs... more The effectiveness of several antioxidant materials in protecting Bacillus thuringiensis var. kurstaki (HD-73) against inactivation by solar irradiation was assessed in the laboratory. The addition of antioxidant materials (UV-absorbers) to B. thuringiensis var. kurstaki (HD-73) formulation prolonged the residual insecticidal activity, resulting in greater effectivness against the potato tuber moth larvae, Phthorimaea operculella.

British Journal of Surgery, 1992

The prognostic power of the extent of tumour invasion is indisputable; Dukes' classification ... more The prognostic power of the extent of tumour invasion is indisputable; Dukes' classification has repeatedly been proven to be strongly correlated with patient survival. Modifications have led only to confusion, resulting in caution being required in the classification of patients with Dukes' A tumours. In the UK, the American tumour node metastasis and Australian clinicopathological systems are frequently considered too complex for routine clinical use. Meanwhile, Jass's classification may be complicated by observer variation between pathologists, and recent evidence suggests that it offers no advantage over that of Dukes. All the conventional staging systems also fail to take the skill of the surgeon into account when determining outcome. Attempts at quantifying tumour structure have not heralded the expected major advance. For instance, the expense and uncertain prognostic value of tumour DNA content assessed by flow cytometry are likely to restrict widespread use of t...

British Journal of Surgery, 1992

Pakistan Journal of Medical and Health Sciences, 2021

Background: The wound dressings perform a crucial role in cutaneous wounds management due to thei... more Background: The wound dressings perform a crucial role in cutaneous wounds management due to their ability to protect wounds and promote dermal and epidermal tissue regeneration. Aim:To evaluate the effectiveness of using hydrogel/nanosilver-based dressing vs. traditional dressing on diabetic foot wound healing. Methods:Sixty patients with type-2 diabetes hospitalized for diabetic foot wound treatment were recruited from selected Surgical departments. A prospective randomized control study was carried Results: The results showed that the percentage of a reduction rate of the ulcer by the third week of the treatment as in the hydrogel/nanosilver-based dressing group was higher (15.11%) than traditional wound dressing group (33.44%). Moreover, the mean ulcers size "sq mm" in the hydrogel/nanosilver-based dressing group recognized a faster healing rate (15.11±7.89), and considerably lesser in comparison to the traditional in the third week (21.65±8.4). Conclusion: The hydroge...

International Society of Offshore and Polar Engineers, May 30, 1999

As might be inferred from the rather formidable subtitle, this book is, in part at least, a treat... more As might be inferred from the rather formidable subtitle, this book is, in part at least, a treatise on scope and method. The first five chapters are entirely so, as shown by their respective titles, namely, "Present Theoretical Positions," "Method," "Principles Applicable to Deductive Classification," "A Positive Definition of Economics," and "Intended Line of Argument." Even the subsequent chapters, while mainly positive and constructive, devote considerable space to the defense of the author's method and point of approach. This emphasis upon definition, scope, and method the author considers necessary, for the reason that he is attempting a reconstruction of economic theory. This reconstruction begins with a complete change in the point of view from which to survey the economic field. This in turn involves a new delimitation of the field itself. His point of view is that of the business man who regards himself as the real producer, with labor, as well as lanid and capital, as mere means of production. His definition of economics, which he has himself italicized, is as follows: Economics is the study of the interrelations of that group of egoistic human activities which are incited by the expectation of definite personal shares, prearranged in supposed conformity to functions performed, of the purchasing power resulting from the joint activity of two or more individuals; and of their outer relations, or how these activities and their results are affected by the physical, ethical, and social environment, and by changes in the environment (p. 65). This definition may not seem very clear to one who has not read the context, but that is too long to reproduce here and the reader must look it up for himself. More significant than the definition, in the opinion of the present writer, is the author's point of view. Being himself a business III

Introduction: Epilepsy is a disease categorized by a spontaneous repetition of unprovoked seizure... more Introduction: Epilepsy is a disease categorized by a spontaneous repetition of unprovoked seizures, is one of the most widespread chronic neurological states. the rate of its prevalence is reported to be 0.7–1.0% (Fiest, Sauro, Wiebe, Patten, Kwon & Dykeman, 2017). The Center for Disease Control and Prevention (CDC) proposes that neurological comorbidities, including epilepsy, could be a risk factor for COVID-19 (Kuroda, 2020). The concern of the COVID19 pandemic toward patients with epilepsy include the fact that Frequent seizures would cause malnutrition, and the nutritional status is associated with the immune system (Crepin, Godet, Chassain, Preux & Desport, 2009). On the other hand, going to emergency rooms because of increased or uncontrollable seizures could expose the patient to coronavirus. Therefore, it's crucial to consider innovative ways to detect and control seizures among these patients. Aim: The current study consists of two folds; the first fold focuses on the comprehensive assessment of adult patients with epilepsy. The second fold of the study describes the innovative nanotechnology epilepsy treating device and suggesting incorporating the proposed device in the nursing management plan of patients with epilepsy. Design: A descriptive research design was espoused in this study. Methods: A convenience sample of 150 adult patients with epilepsy were recruited. Data collection was conducted at the neurology department Kasr Al-Aini University Hospital, and two tools were used to collect the needed data: Sociodemographic datasheet & Comprehensive assessment sheet. The study's second fold is porposed based upon extensive litreture review and the results obtained from the first fold of the study. Results: The majority of the studied subjects, 82%, had epilepsy for more than 5 years up to 10 years. Almost half of the studied subjects, 49.3%, had 3 epileptic attacks the year before the study. Concerning post-ictal symptoms, 93.3% of the study subjects had headaches or migraines, and 52.9% experienced it severely. In addition to 86% of the studied sample felt fear and anxiety. The presence of aura or warning symptoms before seizure attacks (55.3%) of the studied subjects never had any. Conclusion and recommendation: The current study results highlight the critical issue of having an epileptic seizure without any warning signs, which encourages the initiation of using the proposed nanotechnology device to detect the attack before it happens. Incorporating such a device in a nursing management plan can have a magnificent prognosis of patients with epilepsy regarding control of attacks and prevent post-ictal devastating symptoms especilly that patients with epilepsy have a greater risk of COVID19 infection and subsequent morbidity and mortality.

Researchgate, 2016

Measuring cell fluorescence using ImageJ Determining the level of cellular fluorescence from fluo... more Measuring cell fluorescence using ImageJ Determining the level of cellular fluorescence from fluorescence microscopy images in ImageJ https://imagej.nih.gov/ij/download.html Open ImageJ. Note: ImageJ may be freely downloaded from [here](http://rsbweb.nih.gov/ij/download.html) Select the cell of interest using any of the drawing/selection tools (i.e. rectangle, circle, polygon or freeform) From the Analyze menu select “set measurements”. Make sure you have area integrated intensity and mean grey value selected (the rest can be ignored). Now select “Measure” from the analyze menu. You should now see a popup box with a stack of values for that first cell. Now go and select a region next to your cell that has no fluroence, this will be your background. Repeat this step for the other cells in the field of view that you want to measure. NB: Size is not important. If you want to be super accurate here take 3+ selections from around the cell. Once you have finished, select all the data in the Results window and copy and paste into a new spreadsheet (or similar program) Use this formula to calculate the corrected total cell fluorescence (CTCF). CTCF = Integrated Density – (Area of selected cell X Mean fluorescence of background readings) Make a graph and your done. Notice that rounded up mitotic cells appear to have a much higher level of staining due to its smaller size concentrating the staining in a smaller space. If you used the raw integrated density you would have data suggesting that the flattened cell has less staining then the rounded up one, when in reality they have a similar level of fluorescence.

Journal of Nanomedicine & Nanotechnology, 2016

Pakistan Journal of Medical and Health Sciences, 2021

Science of Advanced Materials, 2017

Gold nanoparticles (AuNPs) were successfully synthesized using an eco-friendly method in a single... more Gold nanoparticles (AuNPs) were successfully synthesized using an eco-friendly method in a single-step reaction with red algae (Laurencia papillosa). In this research we investigated the factors that affect the characteristics of AuNPs, such as the size, shape, surface profile and surface chemistry. The synthesized AuNPs were characterized using UV-Visible spectroscopy, X-ray diffraction (XRD), Transmission Electron Microscope (TEM), Field Emission Scanning Electron microscope (FESEM), and Atomic Force Microscope (AFM) measurements. Computational analysis of the size distribution, surface profile, area and value of the different AuNPs synthesized were carried out using freeware ImageJ. The study revealed that the color of the solutions of HAuCl 4 was changed from yellow to ruby red indicating that metallic gold nanoparticles were synthesized. TEM images of the synthesized AuNPs showed different shapes and sizes ranging from 3.5 to 53 nm at max = 586 nm in different concentrations (Graphical Abstract). The resulting shapes were spherical and triangular crystalline gold nanoparticles with varied oxidation states of Au (0) and Au (+1). One of our future goals in Plant Virology is the use of these gold nanoparticles as a carrier in biological control of plant virus diseases using viral satellite RNA as a biological control agent against severe plant virus diseases (United States Patent No. US 8,138,390 B2). The functional groups in the algal extract responsible for the synthesis of the AuNPs were identified to be NH + 2 and OH − groups. The optimum ratio of Au/Algal extract required for producing small sizes of AuNPs was found to be 1:2. The Algal water-extract has dual functions in this synthesis: they act as reducing and stabilizing agent. AuNPs have many biological, industrial and medical applications which include treatments against plant virus and cancer diseases.

CA: A Cancer Journal for Clinicians, 1997

A national task force consisting of members from the American College of Radiology, the American ... more A national task force consisting of members from the American College of Radiology, the American College of Surgeons, and the College of American Pathologists examined the issues surrounding stereotactic core-needle biopsy for occult breast lesions. Their report includes indications and contraindications, informed consent, specimen handling, and management of indeterminate, atypical, or discordant lesions.

Vegetos- An International Journal of Plant Research, 2017

Histopathology of Chloroplast Ultrastructure and Inclusion Bodies in CMV-Infected Plant Tissues U... more Histopathology of Chloroplast Ultrastructure and Inclusion Bodies in CMV-Infected Plant Tissues Using Electron Microscopic Examination and ImageJ Analysis Cucumber mosaic virus (CMV) is an economically important virus that causes wide-spread diseases damaging crops worldwide. Two strains of the virus were detected using ELISA with specific antibodies, and isolated from Kuwait environment designated CMVKU1 and CMV-KU2. The KU1 is a mild strain and is associated with a benign viral satellite RNA, while KU2 isolate, is a severe strain and lacking viral satellite RNA. Both strains were used in a comparative study to investigate the effects of CMV infection on the ultrastructure of chloroplasts and inclusion bodies in test plants of tomato (Lycopersicon esculentum) and squash (Cucurbita pepo). Transmission electron microscopy (TEM) was used to determine the extent of infection and damage caused by virus isolates on chloroplast structure, and cellular inclusions including plastoglobules, and starch granules. Chloroplasts, the photosynthetic apparatus of plants, in infected tissues were severely affected with KU2 strain that caused a reduction and damage of their main structure, number, size, and inclusion bodies compared to the benign strain of KU1 and healthy control plants. Electron micrographs of ultra-thin sections proved the presence of the viral nanoparticles. The formation of viral crystalline bodies, chloroplast damage and malformation due to the viral infection. As the infection progressed, chloroplasts were destroyed and grana were disorganized and scattered into the cytoplasm that causes reduction in chlorophyll contents in plant tissues. ImageJ freeware analysis were used to determine the extent of infection and host-virus interactions and damage caused by virus isolates...

European Journal of Molecular and Clinical Medicine, 2021

Introduction: Epilepsy is a disease categorized by a spontaneous repetition of unprovoked seizure... more Introduction: Epilepsy is a disease categorized by a spontaneous repetition of unprovoked seizures, is one of the most widespread chronic neurological states. the rate of its prevalence is reported to be 0.7–1.0% (Fiest, Sauro, Wiebe, Patten, Kwon & Dykeman, 2017). The Center for Disease Control and Prevention (CDC) proposes that neurological comorbidities, including epilepsy, could be a risk factor for COVID-19 (Kuroda, 2020). The concern of the COVID19 pandemic toward patients with epilepsy include the fact that Frequent seizures would cause malnutrition, and the nutritional status is associated with the immune system (Crepin, Godet, Chassain, Preux & Desport, 2009). On the other hand, going to emergency rooms because of increased or uncontrollable seizures could expose the patient to coronavirus. Therefore, it's crucial to consider innovative ways to detect and control seizures among these patients.
Aim: The current study consists of two folds; the first fold focuses on the comprehensive assessment of adult patients with epilepsy. The second fold of the study describes the innovative nanotechnology epilepsy treating device and suggesting incorporating the proposed device in the nursing management plan of patients with epilepsy. Design: A descriptive research design was espoused in this study. Methods: A convenience sample of 150 adult patients with epilepsy were recruited. Data collection was conducted at the neurology department Kasr Al-Aini University Hospital, and two tools were used to collect the needed data: Sociodemographic datasheet & Comprehensive assessment sheet. The study's second fold is porposed based upon extensive litreture review and the results obtained from the first fold of the study. Results: The majority of the studied subjects, 82%, had epilepsy for more than 5 years up to 10 years. Almost half of the studied subjects, 49.3%, had 3 epileptic attacks the year before the study. Concerning post-ictal symptoms, 93.3% of the study subjects had headaches or migraines, and 52.9% experienced it severely. In addition to 86% of the studied sample felt fear and anxiety. The presence of aura or warning symptoms before seizure attacks (55.3%) of the studied subjects never had any. Conclusion and recommendation: The current study results highlight the critical issue of having an epileptic seizure without any warning signs, which encourages the initiation of using the proposed nanotechnology device to detect the attack before it happens. Incorporating such a device in a nursing management plan can have a magnificent prognosis of patients with epilepsy regarding control of attacks and prevent post-ictal devastating symptoms especilly that patients with epilepsy have a greater risk of COVID19 infection and subsequent morbidity and mortality.

This is the first report on a rapid green synthesis of gold nanoparticles (GNPs) using red algae ... more This is the first report on a rapid green synthesis of gold nanoparticles (GNPs) using red algae (Laurencia papillosa). The green synthesis of eco-friendly nanoparticles is of a great interest in nanoscience for biomedical applications and specifically for clinical diagnostic applications. GNPs especially nanoprism represents a new advanced tool to study cell function and useful in optoelectronics, in developing a drug delivery system to control plant virus diseases and in nanomedicine. Conventional physical and chemical methods have been developed for the synthesis of metal nanoparticles, but these methods are expensive and require the use of toxic and aggressive chemicals. In this paper, it is demonstrated that a rapid, low coast and eco-friendly method for synthesis of gold nanospheres and its conversion into gold nanoprisms has been developed. The method involves using water solvent extract of L. papillosa as a reducing agent. Nanoscopy and computational analysis revealed that the nanoprism and other different morphologies were obtained just by varying the concentration molarity of tetrachloauric acid (HAuCl 4), keeping the concentration of pure algal extract (PAE) constant. In the same time, we perform ImageJ analyses for the size distribution of TEM images. The best concentration of AuCl 4 was 5 mM and best concentration of the red algae extract was at 0.05 g/ml. The functional groups responsible for conversion of nanosphere into nanoprism were NH and OH groups found in the contents of the red algae extract. The as-synthesized gold nanoprisms were characterized by several physicochemical techniques. The nanoprisms are single crystalline, whose basal plates surface are atomically flat "111" planes. We anticipate our results to be a starting point for more applications in medicine, plant virus diseases, and industrial technology.

This is the first report on a rapid green synthesis of gold nanoparticles (GNPs) using red algae ... more This is the first report on a rapid green synthesis of gold nanoparticles (GNPs) using red algae (Laurencia papillosa). The green synthesis of eco-friendly nanoparticles is of a great interest in Nanoscience for biomedical applications and specifically for clinical diagnostic applications. GNPs especially nanoprism represents a new advanced tool to study cell function and useful in optoelectronics, in developing a drug delivery system to control plant virus diseases and in nanomedicine. Conventional physical and chemical methods have been developed for the synthesis of metal nanoparticles, but these methods are expensive and require the use of toxic and aggressive chemicals. In this paper it is demonstrated that a rapid, low coast and ecofriendly method for synthesis of gold nanosphere and its conversion into gold nanoprism has been developed. The method involves using water solvent extract of Laurencia papillosa as a reducing agent. Nanoscopy analysis revealed that the nanoprism and other different morphologies were obtained just by varying the concentration molarity of tetrachloauric acid (HAuCl 4), keeping the concentration of pure algal extract (PAE) constant. The best concentration of AuCl 4 was 5 mM and best concentration of the red algae extract was at 0.05 g/ml. The functional groups responsible for conversion of nanospheres into nanoprism were NH and OH groups found in the contents of the red algae extract. The as-synthesized gold nanoprisms were characterized by several physico-chemical techniques. The nanoprisms are single crystalline, whose basal plates surface are atomically flat "111" planes. We anticipate our results to be a starting point for more applications in medicine and industrial technology.

Australian Journal of Basic and Applied Sciences

British Journal of Surgery, 1992

Pakistan Journal of Medical and Health Sciences, 2021

International Society of Offshore and Polar Engineers, May 30, 1999

Researchgate, 2016

Journal of Nanomedicine & Nanotechnology, 2016

Pakistan Journal of Medical and Health Sciences, 2021

Science of Advanced Materials, 2017

CA: A Cancer Journal for Clinicians, 1997

Vegetos- An International Journal of Plant Research, 2017

European Journal of Molecular and Clinical Medicine, 2021

Introduction: Epilepsy is a disease categorized by a spontaneous repetition of unprovoked seizure... more Introduction: Epilepsy is a disease categorized by a spontaneous repetition of unprovoked seizures, is one of the most widespread chronic neurological states. the rate of its prevalence is reported to be 0.7–1.0% (Fiest, Sauro, Wiebe, Patten, Kwon & Dykeman, 2017). The Center for Disease Control and Prevention (CDC) proposes that neurological comorbidities, including epilepsy, could be a risk factor for COVID-19 (Kuroda, 2020). The concern of the COVID19 pandemic toward patients with epilepsy include the fact that Frequent seizures would cause malnutrition, and the nutritional status is associated with the immune system (Crepin, Godet, Chassain, Preux & Desport, 2009). On the other hand, going to emergency rooms because of increased or uncontrollable seizures could expose the patient to coronavirus. Therefore, it's crucial to consider innovative ways to detect and control seizures among these patients.
Aim: The current study consists of two folds; the first fold focuses on the comprehensive assessment of adult patients with epilepsy. The second fold of the study describes the innovative nanotechnology epilepsy treating device and suggesting incorporating the proposed device in the nursing management plan of patients with epilepsy. Design: A descriptive research design was espoused in this study. Methods: A convenience sample of 150 adult patients with epilepsy were recruited. Data collection was conducted at the neurology department Kasr Al-Aini University Hospital, and two tools were used to collect the needed data: Sociodemographic datasheet & Comprehensive assessment sheet. The study's second fold is porposed based upon extensive litreture review and the results obtained from the first fold of the study. Results: The majority of the studied subjects, 82%, had epilepsy for more than 5 years up to 10 years. Almost half of the studied subjects, 49.3%, had 3 epileptic attacks the year before the study. Concerning post-ictal symptoms, 93.3% of the study subjects had headaches or migraines, and 52.9% experienced it severely. In addition to 86% of the studied sample felt fear and anxiety. The presence of aura or warning symptoms before seizure attacks (55.3%) of the studied subjects never had any. Conclusion and recommendation: The current study results highlight the critical issue of having an epileptic seizure without any warning signs, which encourages the initiation of using the proposed nanotechnology device to detect the attack before it happens. Incorporating such a device in a nursing management plan can have a magnificent prognosis of patients with epilepsy regarding control of attacks and prevent post-ictal devastating symptoms especilly that patients with epilepsy have a greater risk of COVID19 infection and subsequent morbidity and mortality.

This is the first report on a rapid green synthesis of gold nanoparticles (GNPs) using red algae ... more This is the first report on a rapid green synthesis of gold nanoparticles (GNPs) using red algae (Laurencia papillosa). The green synthesis of eco-friendly nanoparticles is of a great interest in Nanoscience for biomedical applications and specifically for clinical diagnostic applications. GNPs especially nanoprism represents a new advanced tool to study cell function and useful in optoelectronics, in developing a drug delivery system to control plant virus diseases and in nanomedicine. Conventional physical and chemical methods have been developed for the synthesis of metal nanoparticles, but these methods are expensive and require the use of toxic and aggressive chemicals. In this paper it is demonstrated that a rapid, low coast and ecofriendly method for synthesis of gold nanosphere and its conversion into gold nanoprism has been developed. The method involves using water solvent extract of Laurencia papillosa as a reducing agent. Nanoscopy analysis revealed that the nanoprism and other different morphologies were obtained just by varying the concentration molarity of tetrachloauric acid (HAuCl 4), keeping the concentration of pure algal extract (PAE) constant. The best concentration of AuCl 4 was 5 mM and best concentration of the red algae extract was at 0.05 g/ml. The functional groups responsible for conversion of nanospheres into nanoprism were NH and OH groups found in the contents of the red algae extract. The as-synthesized gold nanoprisms were characterized by several physico-chemical techniques. The nanoprisms are single crystalline, whose basal plates surface are atomically flat "111" planes. We anticipate our results to be a starting point for more applications in medicine and industrial technology.