Agnieszka A Chlebicz-Wójcik | Technical University of Lodz (original) (raw)
Papers by Agnieszka A Chlebicz-Wójcik
Postępy Mikrobiologii. Suplement, 2017
Fems Microbiology Letters, Jul 11, 2019
Probiotics and Antimicrobial Proteins, Feb 5, 2019
The aim of the following research was to determine the detoxification properties of probiotic Lac... more The aim of the following research was to determine the detoxification properties of probiotic Lactobacillus sp. bacteria (12 strains) and S. cerevisiae yeast (6 strains) towards mycotoxins, such as aflatoxin B 1 , deoxynivalenol, fumonisins, T-2 toxin and zearalenone, which pose as frequent feed contamination. The experiment involved analysing changes in concentration of mycotoxins in PBS solutions, after 6, 12 and 24 h of incubation with monocultures of tested microorganisms, measured by high-performance liquid chromatography (HPLC). We found that all strains detoxified the mycotoxins, with the highest reduction in concentration observed for the fumonisin B 1 and B 2 mixture, ranging between 62 and 77% for bacterial strains and 67-74% for yeast. By contrast, deoxynivalenol was the most resistant mycotoxin: its concentration was reduced by 19-39% by Lactobacillus sp. strains and 22-43% by yeast after 24 h of incubation. High detoxification rates for aflatoxin B 1 , T-2 toxin and zearalenone were also observed, with concentration reduced on average by 60%, 61% and 57% by Lactobacillus, respectively, and 65%, 69% and 52% by yeast, respectively. The greatest extent of reduction in the concentration for all mycotoxins was observed after 6 h of incubation; however, a decrease in concentration was noted even after 24 h of incubation. Thus, the tested microorganisms can potentially be used as additives to decrease the concentrations of toxins in animal feed.
Żywność, 2019
Probiotics including their combinations with prebiotics known as synbiotics have been extensively... more Probiotics including their combinations with prebiotics known as synbiotics have been extensively studied as an alternatives to antibiotic growth promoters commonly used in livestock breeding. Among many microorganisms that may be useful for their host, the Saccharomyces cerevisiae yeast exhibits probiotic potential. The objective of the research study was to select a S. cerevisiae strain suitable for use as a probiotic in newly developed synbiotic preparations for monogastric animals (poultry, swine). The survivability of yeast strains in the presence of bile salts and under low-pH conditions varied and after 4 h of incubation it ranged from 66 to 94 % and from 68 to 97 %, respectively. The S. cerevisiae ŁOCK 0119 strain was characterised by the highest survival rate; the number of live cells of this strain was reduced not more than 9 % after 4 h of incubation under the fixed conditions. Also, this strain exhibited hydrophilic properties and a strong auto-and co-aggregation potential. The selected yeast strain aggregated with pathogenic bacteria (Salmonella spp., Listeria monocytogenes) to a varying degree ranging between 40 and 75 %. The S. cerevisiae ŁOCK 0119 strain inhibited the adhesion of pathogens to the Caco-2 cells, whereby the adhesion of morbific bacteria attachment was reduced to a varying degree (15 ÷ 37 %). Based on the results obtained, it was concluded that the selected S. cerevisiae yeast strain had beneficial properties since it was able to survive during gastrointestinal passage, to colonize the intestines and to reduce the growth of pathogenic microbiota.
Journal of the Science of Food and Agriculture, Apr 1, 2019
BACKGROUND: The administration of probiotics and prebiotics (synbiotics) is a promising method fo... more BACKGROUND: The administration of probiotics and prebiotics (synbiotics) is a promising method for detoxification of ochratoxin A in animals. The aim of this study was to investigate the ability of five probiotic strains of lactic acid bacteria (LAB) and one S. cerevisiae yeast strain, from three different synbiotics for poultry, to detoxify OTA. In addition, we also investigated the genotoxicity of faecal water (FW) of chickens after administering OTA and/or synbiotics for 42 days. RESULTS: All tested LAB and yeast strains had the ability to detoxify OTA by significant (P < 0.05) reducing its concentration (by 31.3-47.7% and 31.9% respectively after 24h incubation) and genotoxicity (by 22.6-51.8% and 52.7% respectively). Synbiotics composed of four and five probiotic strains significantly (P < 0.05) decreased FW genotoxicity of chicks, after exposure to OTA, to the level seen in the control group (21.8 ± 1.7%) and were more effective than synbiotics composed of three probiotic strains (31.5%). CONCLUSION: These results showed that there was a beneficial effect of the synbiotics on the gastrointestinal tract of animals. Furthermore, synbiotic preparations containing four or five of tested strains can be considered as preventive agents in the contamination of poultry with OTA.
Animals, Oct 30, 2020
Widespread antibiotic resistance among microorganisms led to a prohibition or limitation of using... more Widespread antibiotic resistance among microorganisms led to a prohibition or limitation of using antibiotic growth promoters in livestock breeding. In order to maintain the animal production on the level which could satisfy the demands, and to reduce the risk of infections occurrence among the livestock, alternative preparations are being searched for. Pro-and prebiotics are wildly studied; however, their combination, which are called synbiotics, are expected to impact animals' health more considerably. There are a number of pro-and prebiotic preparations available on the market; nevertheless, synbiotics are rare, which is why this research was focused on their impact on pigs' intestinal microbiota and organic acids synthesis. The results showed that newly developed synbiotics could have a more beneficial impact on piglets' health rather than commercial probiotics.
Nutrients, Apr 30, 2020
Prebiotics are food components that are selectively fermented by beneficial microbiota and which ... more Prebiotics are food components that are selectively fermented by beneficial microbiota and which confer a health benefit. The aim of the study was to select a prebiotic for the chosen probiotic strains to create a synbiotic. The impact of prebiotics (inulin, maltodextrin, corn starch, β-glucan, and apple pectin) on five Lactobacillus spp. strains' growth and metabolites synthesis (lactic, acetic, propionic, and butyric acids, ethanol, and acetaldehyde) was tested by the plate count method and by high-performance liquid chromatography, respectively. Moreover, the differences in the ratio of D(−) and L(+) lactate isomers produced by Lactobacillus spp., as well as variations in the probiotics' enzymatic profiles associated with the prebiotic used for cultivation, were determined with a Megazyme rapid assay kit and API ® ZYM assay, accordingly. Finally, the influence of the carbon source (prebiotic) used on the antagonistic activity of the probiotic strains towards pathogenic bacteria, such as Salmonella spp. or Listeria monocytogenes was analyzed in the co-cultures. The results showed that the growth, metabolic profile, and antagonistic activity of the probiotics towards selected pathogens were the most favorable when 2% (w/v) of inulin was used. Therefore, the combination of inulin with selected probiotics is a promising synbiotic mixture.
International Journal of Environmental Research and Public Health, 2018
Zoonoses are diseases transmitted from animals to humans, posing a great threat to the health and... more Zoonoses are diseases transmitted from animals to humans, posing a great threat to the health and life of people all over the world. According to WHO estimations, 600 million cases of diseases caused by contaminated food were noted in 2010, including almost 350 million caused by pathogenic bacteria. Campylobacter, Salmonella, as well as Yersinia enterocolitica and Listeria monocytogenes may dwell in livestock (poultry, cattle, and swine) but are also found in wild animals, pets, fish, and rodents. Animals, often being asymptomatic carriers of pathogens, excrete them with faeces, thus delivering them to the environment. Therefore, pathogens may invade new individuals, as well as reside on vegetables and fruits. Pathogenic bacteria also penetrate food production areas and may remain there in the form of a biofilm covering the surfaces of machines and equipment. A common occurrence of microbes in food products, as well as their improper or careless processing, leads to common poisoning...
Wpływ temperatury dojrzewania na mikroflorę serów podpuszczkowych przygotowywanych z niepasteryzo... more Wpływ temperatury dojrzewania na mikroflorę serów podpuszczkowych przygotowywanych z niepasteryzowanego mleka krowiego……………………………………………………….
Zywnosc Nauka Technologia Jakosc/Food Science Technology Quality, 2019
Probiotics including their combinations with prebiotics known as synbiotics have been extensively... more Probiotics including their combinations with prebiotics known as synbiotics have been extensively studied as an alternatives to antibiotic growth promoters commonly used in livestock breeding. Among many microorganisms that may be useful for their host, the Saccharomyces cerevisiae yeast exhibits probiotic potential. The objective of the research study was to select a S. cerevisiae strain suitable for use as a probiotic in newly developed synbiotic preparations for monogastric animals (poultry, swine). The survivability of yeast strains in the presence of bile salts and under low-pH conditions varied and after 4 h of incubation it ranged from 66 to 94 % and from 68 to 97 %, respectively. The S. cerevisiae ŁOCK 0119 strain was characterised by the highest survival rate; the number of live cells of this strain was reduced not more than 9 % after 4 h of incubation under the fixed conditions. Also, this strain exhibited hydrophilic properties and a strong auto- and co-aggregation potent...
Biomolecules
Irritable bowel syndrome is not a life-threatening disease, yet it significantly affects the qual... more Irritable bowel syndrome is not a life-threatening disease, yet it significantly affects the quality of life and contributes to economic loss. It is estimated that even up to 45% of the world’s population can suffer from the disease. The first attempts to diagnose irritable bowel syndrome were made at the end of the 19th century; however, establishing appropriate diagnostic criteria and treatment methods is still ongoing. To date, little is known about the etiology of irritable bowel syndrome; however, growing attention is drawn to the intestinal microbiota as a factor in the disease development. For this reason, researchers have conducted many studies on therapies that modulate the microbiota, among which probiotics, prebiotics, and synbiotics are widely studied. To date, most studies have examined probiotics; however, there are also several studies demonstrating the efficacy of prebiotics and synbiotics. The aim of this review was to summarize findings on the usefulness of probiot...
Biology
The growing need for Lactobacillus bacteria usage in industry and the expending probiotic market ... more The growing need for Lactobacillus bacteria usage in industry and the expending probiotic market led to a search for new cost-efficient fermentation media from which a high yield of these bacteria could be obtained. The following study aimed to elaborate cultivation medium, for Lactobacillus spp. growth, which main components would be wheat, maize, barley, and rye flours. The optimal temperature for Lactobacillus growth in new semi-solid fermentation (SSF) medium, water content, and pH of the medium were analyzed by the plate count method. It was established, that the highest bacteria counts were obtained from cultures conducted in the SSF medium with flours to water ratio of 1:1.5 with a natural pH of 6.0 at 37 °C. Subsequently, the growth kinetics of analyzed strains, in both MRS and the SSF media, were studied. The newly designed media contributed to the increased duration of selected Lactobacillus strains lag phase, which varied from 1.98 to 5.64; nevertheless, the maximum growt...
Animals
The study was conducted to determine the influence of newly elaborated synbiotic preparations on ... more The study was conducted to determine the influence of newly elaborated synbiotic preparations on piglets’ intestinal microbiota and its metabolism. Animals were distributed among six experimental groups, in reference to used feed supplements, namely, synbiotics (A, B, or C) or commercially available probiotics (BioPlus 2B®, Chr. Hansen A/S, Horsholm, Denmark or Cylactin® LBC, DSM Nutritional Products Ltd., Kaiseraugst, Switzerland), or its absence (control group). Until the 29th day of life, piglets were breastfed by sows, whose feed was supplemented, and fecal samples were collected at the 7th and 28th day of piglets’ life. After weaning of the piglets, the research was continued until the 165th day of the pigs’ life. The area of this work included the analysis of the piglets’ dominant fecal microbiota by the plate count method. Moreover, high-performance liquid chromatography analysis (HPLC) was applied to establish variations in the concentrations of organic acids, namely, lactic...
Nutrients
Prebiotics are food components that are selectively fermented by beneficial microbiota and which ... more Prebiotics are food components that are selectively fermented by beneficial microbiota and which confer a health benefit. The aim of the study was to select a prebiotic for the chosen probiotic strains to create a synbiotic. The impact of prebiotics (inulin, maltodextrin, corn starch, β-glucan, and apple pectin) on five Lactobacillus spp. strains’ growth and metabolites synthesis (lactic, acetic, propionic, and butyric acids, ethanol, and acetaldehyde) was tested by the plate count method and by high-performance liquid chromatography, respectively. Moreover, the differences in the ratio of D(−) and L(+) lactate isomers produced by Lactobacillus spp., as well as variations in the probiotics’ enzymatic profiles associated with the prebiotic used for cultivation, were determined with a Megazyme rapid assay kit and API® ZYM assay, accordingly. Finally, the influence of the carbon source (prebiotic) used on the antagonistic activity of the probiotic strains towards pathogenic bacteria, s...
Probiotics and Antimicrobial Proteins
The study aimed to evaluate the safety and probiotic properties of selected Lactobacillus strains... more The study aimed to evaluate the safety and probiotic properties of selected Lactobacillus strains, which are intended to be fed to monogastric animals. The Lactobacillus spp. appeared to be safe since they did not degrade mucus and did not exhibit β-haemolysis. Moreover, the survival of Caco-2 cells in the presence of metabolites of the selected strains was high, which also indicated their safety. The analysed strains showed moderate or strong antagonistic activity against Salmonella spp., Listeria monocytogenes, Campylobacter jejuni and Campylobacter coli, which was tested with the usage of the agar slab method. Furthermore, the strains showed high survivability in an acidic environment and the presence of bile salts (~90%). High resistivity or moderate susceptibility to antibiotics was also observed, as a result of the disc diffusion method. The strains were mostly moderately hydrophilic (hydrophobicity: 10.43–41.14%); nevertheless, their auto-aggregation capability exceeded 50% a...
FEMS Microbiology Letters, 2016
The aim of this study was to estimate the influence of synbiotics on intestinal microbiota and it... more The aim of this study was to estimate the influence of synbiotics on intestinal microbiota and its metabolism in sows. Three different synbiotics were administered with feed to animals from three experimental groups. Two groups of sows were given commercially available probiotics (BioPlus 2B®, Cylactin® LBC) as forage additives for comparison. The control group of sows was given unmodified fodder. The study was conducted for 48 days (10 days before farrowing, and continued 38 days after) and faeces samples were collected four times. The scope of this work was to designate the dominant microbiota in sows’ faeces. Therefore, the total number of anaerobic bacteria, Bifidobacterium sp., Lactobacillus sp., Bacteroides sp., Clostridium sp., Enterococcus sp., Enterobacteriaceae, Escherichia coli and yeast was determined, using the plate method. Changes in the concentration of lactic acid, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were also determined in correla...
Postępy Mikrobiologii. Suplement, 2017
Fems Microbiology Letters, Jul 11, 2019
Probiotics and Antimicrobial Proteins, Feb 5, 2019
The aim of the following research was to determine the detoxification properties of probiotic Lac... more The aim of the following research was to determine the detoxification properties of probiotic Lactobacillus sp. bacteria (12 strains) and S. cerevisiae yeast (6 strains) towards mycotoxins, such as aflatoxin B 1 , deoxynivalenol, fumonisins, T-2 toxin and zearalenone, which pose as frequent feed contamination. The experiment involved analysing changes in concentration of mycotoxins in PBS solutions, after 6, 12 and 24 h of incubation with monocultures of tested microorganisms, measured by high-performance liquid chromatography (HPLC). We found that all strains detoxified the mycotoxins, with the highest reduction in concentration observed for the fumonisin B 1 and B 2 mixture, ranging between 62 and 77% for bacterial strains and 67-74% for yeast. By contrast, deoxynivalenol was the most resistant mycotoxin: its concentration was reduced by 19-39% by Lactobacillus sp. strains and 22-43% by yeast after 24 h of incubation. High detoxification rates for aflatoxin B 1 , T-2 toxin and zearalenone were also observed, with concentration reduced on average by 60%, 61% and 57% by Lactobacillus, respectively, and 65%, 69% and 52% by yeast, respectively. The greatest extent of reduction in the concentration for all mycotoxins was observed after 6 h of incubation; however, a decrease in concentration was noted even after 24 h of incubation. Thus, the tested microorganisms can potentially be used as additives to decrease the concentrations of toxins in animal feed.
Żywność, 2019
Probiotics including their combinations with prebiotics known as synbiotics have been extensively... more Probiotics including their combinations with prebiotics known as synbiotics have been extensively studied as an alternatives to antibiotic growth promoters commonly used in livestock breeding. Among many microorganisms that may be useful for their host, the Saccharomyces cerevisiae yeast exhibits probiotic potential. The objective of the research study was to select a S. cerevisiae strain suitable for use as a probiotic in newly developed synbiotic preparations for monogastric animals (poultry, swine). The survivability of yeast strains in the presence of bile salts and under low-pH conditions varied and after 4 h of incubation it ranged from 66 to 94 % and from 68 to 97 %, respectively. The S. cerevisiae ŁOCK 0119 strain was characterised by the highest survival rate; the number of live cells of this strain was reduced not more than 9 % after 4 h of incubation under the fixed conditions. Also, this strain exhibited hydrophilic properties and a strong auto-and co-aggregation potential. The selected yeast strain aggregated with pathogenic bacteria (Salmonella spp., Listeria monocytogenes) to a varying degree ranging between 40 and 75 %. The S. cerevisiae ŁOCK 0119 strain inhibited the adhesion of pathogens to the Caco-2 cells, whereby the adhesion of morbific bacteria attachment was reduced to a varying degree (15 ÷ 37 %). Based on the results obtained, it was concluded that the selected S. cerevisiae yeast strain had beneficial properties since it was able to survive during gastrointestinal passage, to colonize the intestines and to reduce the growth of pathogenic microbiota.
Journal of the Science of Food and Agriculture, Apr 1, 2019
BACKGROUND: The administration of probiotics and prebiotics (synbiotics) is a promising method fo... more BACKGROUND: The administration of probiotics and prebiotics (synbiotics) is a promising method for detoxification of ochratoxin A in animals. The aim of this study was to investigate the ability of five probiotic strains of lactic acid bacteria (LAB) and one S. cerevisiae yeast strain, from three different synbiotics for poultry, to detoxify OTA. In addition, we also investigated the genotoxicity of faecal water (FW) of chickens after administering OTA and/or synbiotics for 42 days. RESULTS: All tested LAB and yeast strains had the ability to detoxify OTA by significant (P < 0.05) reducing its concentration (by 31.3-47.7% and 31.9% respectively after 24h incubation) and genotoxicity (by 22.6-51.8% and 52.7% respectively). Synbiotics composed of four and five probiotic strains significantly (P < 0.05) decreased FW genotoxicity of chicks, after exposure to OTA, to the level seen in the control group (21.8 ± 1.7%) and were more effective than synbiotics composed of three probiotic strains (31.5%). CONCLUSION: These results showed that there was a beneficial effect of the synbiotics on the gastrointestinal tract of animals. Furthermore, synbiotic preparations containing four or five of tested strains can be considered as preventive agents in the contamination of poultry with OTA.
Animals, Oct 30, 2020
Widespread antibiotic resistance among microorganisms led to a prohibition or limitation of using... more Widespread antibiotic resistance among microorganisms led to a prohibition or limitation of using antibiotic growth promoters in livestock breeding. In order to maintain the animal production on the level which could satisfy the demands, and to reduce the risk of infections occurrence among the livestock, alternative preparations are being searched for. Pro-and prebiotics are wildly studied; however, their combination, which are called synbiotics, are expected to impact animals' health more considerably. There are a number of pro-and prebiotic preparations available on the market; nevertheless, synbiotics are rare, which is why this research was focused on their impact on pigs' intestinal microbiota and organic acids synthesis. The results showed that newly developed synbiotics could have a more beneficial impact on piglets' health rather than commercial probiotics.
Nutrients, Apr 30, 2020
Prebiotics are food components that are selectively fermented by beneficial microbiota and which ... more Prebiotics are food components that are selectively fermented by beneficial microbiota and which confer a health benefit. The aim of the study was to select a prebiotic for the chosen probiotic strains to create a synbiotic. The impact of prebiotics (inulin, maltodextrin, corn starch, β-glucan, and apple pectin) on five Lactobacillus spp. strains' growth and metabolites synthesis (lactic, acetic, propionic, and butyric acids, ethanol, and acetaldehyde) was tested by the plate count method and by high-performance liquid chromatography, respectively. Moreover, the differences in the ratio of D(−) and L(+) lactate isomers produced by Lactobacillus spp., as well as variations in the probiotics' enzymatic profiles associated with the prebiotic used for cultivation, were determined with a Megazyme rapid assay kit and API ® ZYM assay, accordingly. Finally, the influence of the carbon source (prebiotic) used on the antagonistic activity of the probiotic strains towards pathogenic bacteria, such as Salmonella spp. or Listeria monocytogenes was analyzed in the co-cultures. The results showed that the growth, metabolic profile, and antagonistic activity of the probiotics towards selected pathogens were the most favorable when 2% (w/v) of inulin was used. Therefore, the combination of inulin with selected probiotics is a promising synbiotic mixture.
International Journal of Environmental Research and Public Health, 2018
Zoonoses are diseases transmitted from animals to humans, posing a great threat to the health and... more Zoonoses are diseases transmitted from animals to humans, posing a great threat to the health and life of people all over the world. According to WHO estimations, 600 million cases of diseases caused by contaminated food were noted in 2010, including almost 350 million caused by pathogenic bacteria. Campylobacter, Salmonella, as well as Yersinia enterocolitica and Listeria monocytogenes may dwell in livestock (poultry, cattle, and swine) but are also found in wild animals, pets, fish, and rodents. Animals, often being asymptomatic carriers of pathogens, excrete them with faeces, thus delivering them to the environment. Therefore, pathogens may invade new individuals, as well as reside on vegetables and fruits. Pathogenic bacteria also penetrate food production areas and may remain there in the form of a biofilm covering the surfaces of machines and equipment. A common occurrence of microbes in food products, as well as their improper or careless processing, leads to common poisoning...
Wpływ temperatury dojrzewania na mikroflorę serów podpuszczkowych przygotowywanych z niepasteryzo... more Wpływ temperatury dojrzewania na mikroflorę serów podpuszczkowych przygotowywanych z niepasteryzowanego mleka krowiego……………………………………………………….
Zywnosc Nauka Technologia Jakosc/Food Science Technology Quality, 2019
Probiotics including their combinations with prebiotics known as synbiotics have been extensively... more Probiotics including their combinations with prebiotics known as synbiotics have been extensively studied as an alternatives to antibiotic growth promoters commonly used in livestock breeding. Among many microorganisms that may be useful for their host, the Saccharomyces cerevisiae yeast exhibits probiotic potential. The objective of the research study was to select a S. cerevisiae strain suitable for use as a probiotic in newly developed synbiotic preparations for monogastric animals (poultry, swine). The survivability of yeast strains in the presence of bile salts and under low-pH conditions varied and after 4 h of incubation it ranged from 66 to 94 % and from 68 to 97 %, respectively. The S. cerevisiae ŁOCK 0119 strain was characterised by the highest survival rate; the number of live cells of this strain was reduced not more than 9 % after 4 h of incubation under the fixed conditions. Also, this strain exhibited hydrophilic properties and a strong auto- and co-aggregation potent...
Biomolecules
Irritable bowel syndrome is not a life-threatening disease, yet it significantly affects the qual... more Irritable bowel syndrome is not a life-threatening disease, yet it significantly affects the quality of life and contributes to economic loss. It is estimated that even up to 45% of the world’s population can suffer from the disease. The first attempts to diagnose irritable bowel syndrome were made at the end of the 19th century; however, establishing appropriate diagnostic criteria and treatment methods is still ongoing. To date, little is known about the etiology of irritable bowel syndrome; however, growing attention is drawn to the intestinal microbiota as a factor in the disease development. For this reason, researchers have conducted many studies on therapies that modulate the microbiota, among which probiotics, prebiotics, and synbiotics are widely studied. To date, most studies have examined probiotics; however, there are also several studies demonstrating the efficacy of prebiotics and synbiotics. The aim of this review was to summarize findings on the usefulness of probiot...
Biology
The growing need for Lactobacillus bacteria usage in industry and the expending probiotic market ... more The growing need for Lactobacillus bacteria usage in industry and the expending probiotic market led to a search for new cost-efficient fermentation media from which a high yield of these bacteria could be obtained. The following study aimed to elaborate cultivation medium, for Lactobacillus spp. growth, which main components would be wheat, maize, barley, and rye flours. The optimal temperature for Lactobacillus growth in new semi-solid fermentation (SSF) medium, water content, and pH of the medium were analyzed by the plate count method. It was established, that the highest bacteria counts were obtained from cultures conducted in the SSF medium with flours to water ratio of 1:1.5 with a natural pH of 6.0 at 37 °C. Subsequently, the growth kinetics of analyzed strains, in both MRS and the SSF media, were studied. The newly designed media contributed to the increased duration of selected Lactobacillus strains lag phase, which varied from 1.98 to 5.64; nevertheless, the maximum growt...
Animals
The study was conducted to determine the influence of newly elaborated synbiotic preparations on ... more The study was conducted to determine the influence of newly elaborated synbiotic preparations on piglets’ intestinal microbiota and its metabolism. Animals were distributed among six experimental groups, in reference to used feed supplements, namely, synbiotics (A, B, or C) or commercially available probiotics (BioPlus 2B®, Chr. Hansen A/S, Horsholm, Denmark or Cylactin® LBC, DSM Nutritional Products Ltd., Kaiseraugst, Switzerland), or its absence (control group). Until the 29th day of life, piglets were breastfed by sows, whose feed was supplemented, and fecal samples were collected at the 7th and 28th day of piglets’ life. After weaning of the piglets, the research was continued until the 165th day of the pigs’ life. The area of this work included the analysis of the piglets’ dominant fecal microbiota by the plate count method. Moreover, high-performance liquid chromatography analysis (HPLC) was applied to establish variations in the concentrations of organic acids, namely, lactic...
Nutrients
Prebiotics are food components that are selectively fermented by beneficial microbiota and which ... more Prebiotics are food components that are selectively fermented by beneficial microbiota and which confer a health benefit. The aim of the study was to select a prebiotic for the chosen probiotic strains to create a synbiotic. The impact of prebiotics (inulin, maltodextrin, corn starch, β-glucan, and apple pectin) on five Lactobacillus spp. strains’ growth and metabolites synthesis (lactic, acetic, propionic, and butyric acids, ethanol, and acetaldehyde) was tested by the plate count method and by high-performance liquid chromatography, respectively. Moreover, the differences in the ratio of D(−) and L(+) lactate isomers produced by Lactobacillus spp., as well as variations in the probiotics’ enzymatic profiles associated with the prebiotic used for cultivation, were determined with a Megazyme rapid assay kit and API® ZYM assay, accordingly. Finally, the influence of the carbon source (prebiotic) used on the antagonistic activity of the probiotic strains towards pathogenic bacteria, s...
Probiotics and Antimicrobial Proteins
The study aimed to evaluate the safety and probiotic properties of selected Lactobacillus strains... more The study aimed to evaluate the safety and probiotic properties of selected Lactobacillus strains, which are intended to be fed to monogastric animals. The Lactobacillus spp. appeared to be safe since they did not degrade mucus and did not exhibit β-haemolysis. Moreover, the survival of Caco-2 cells in the presence of metabolites of the selected strains was high, which also indicated their safety. The analysed strains showed moderate or strong antagonistic activity against Salmonella spp., Listeria monocytogenes, Campylobacter jejuni and Campylobacter coli, which was tested with the usage of the agar slab method. Furthermore, the strains showed high survivability in an acidic environment and the presence of bile salts (~90%). High resistivity or moderate susceptibility to antibiotics was also observed, as a result of the disc diffusion method. The strains were mostly moderately hydrophilic (hydrophobicity: 10.43–41.14%); nevertheless, their auto-aggregation capability exceeded 50% a...
FEMS Microbiology Letters, 2016
The aim of this study was to estimate the influence of synbiotics on intestinal microbiota and it... more The aim of this study was to estimate the influence of synbiotics on intestinal microbiota and its metabolism in sows. Three different synbiotics were administered with feed to animals from three experimental groups. Two groups of sows were given commercially available probiotics (BioPlus 2B®, Cylactin® LBC) as forage additives for comparison. The control group of sows was given unmodified fodder. The study was conducted for 48 days (10 days before farrowing, and continued 38 days after) and faeces samples were collected four times. The scope of this work was to designate the dominant microbiota in sows’ faeces. Therefore, the total number of anaerobic bacteria, Bifidobacterium sp., Lactobacillus sp., Bacteroides sp., Clostridium sp., Enterococcus sp., Enterobacteriaceae, Escherichia coli and yeast was determined, using the plate method. Changes in the concentration of lactic acid, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were also determined in correla...